CN110845266A - Embryonic germ cell dedifferentiation culture medium and preparation method thereof - Google Patents
Embryonic germ cell dedifferentiation culture medium and preparation method thereof Download PDFInfo
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- CN110845266A CN110845266A CN201911158172.0A CN201911158172A CN110845266A CN 110845266 A CN110845266 A CN 110845266A CN 201911158172 A CN201911158172 A CN 201911158172A CN 110845266 A CN110845266 A CN 110845266A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
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Abstract
The invention discloses a germ cell dedifferentiation culture medium, which comprises the following components in percentage by weight: MS, 6-8g/L of modified agar, 20-26g/L of glucose, 0.5-1.1mg/L of 2, 4-dichlorophenoxyacetic acid, 0.5-1.1mg/L of fructose, 0.2-0.7mg/L of nutrient solution, 5-15g/L of modified montmorillonite, and pH value of 5.6-5.8. According to the germ cell dedifferentiation culture medium, agar is modified by a sodium molybdate solution on a conventional basis, so that the nutrient medium energy of the germ cell dedifferentiation culture medium can be enhanced, the antibacterial environment of the medium can be improved, the culture medium can provide various trace elements by adding a nutrient solution, the differentiation degree of the germ cells is improved, and a Chinese herbal medicine leavening agent is added in the form of a nutrient, so that plants can be continuously in a sterilized environment when absorbing nutrition.
Description
Technical Field
The invention relates to the technical field of germ cells, in particular to a germ cell dedifferentiation culture medium and a preparation method thereof.
Background
Soybean is one of the important grain crops in China, has been cultivated for five thousand years, is called Shushu in ancient times, and is a crop with seeds containing rich plant protein, wherein northeast China is the main production area. Soybeans are most commonly used for making various bean products, extracting soybean oil, brewing soy sauce, and extracting proteins. Okara or soybeans ground into meal are also commonly used in livestock feed. The beans are commonly called soybeans. Glycine of Leguminosae family belongs to annual herb, and is 30-90 cm high. Soybean pods are hypertrophic, slightly curved, drooping, yellowish green, and densely covered with brownish yellow and hairy hair; 2-5 seeds, oval, nearly spherical, smooth seed coat, light green, yellow, brown and black, etc. The flowering period is 6-7 months, and the fruit period is 7-9 months.
The soybean germ cells need to use a culture medium in the dedifferentiation process, and the existing culture medium can provide nutrient substances in the dedifferentiation process of the germ cells, but has no antibacterial and sterilizing capabilities and easily influences the dedifferentiation effect of the germ cells.
Disclosure of Invention
The present invention aims to provide a culture medium for dedifferentiation of germ cells and a preparation method thereof, so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
the embryonic cell dedifferentiation culture medium comprises the following components in percentage by weight: MS, 6-8g/L of modified agar, 20-26g/L of glucose, 0.5-1.1mg/L of 2, 4-dichlorophenoxyacetic acid, 0.5-1.1mg/L of fructose, 0.2-0.7mg/L of nutrient solution, 5-15g/L of modified montmorillonite, and pH value of 5.6-5.8.
Preferably, the preparation method of the modified agar comprises the following steps: irradiating the agar by ultraviolet for 10-20min, then blending the agar and a sodium molybdate solution for 10-20min at the blending rotation speed of 200-280r/min, filtering after blending, and then drying.
Preferably, the power of the ultraviolet irradiation is 20-100W.
Preferably, the nutrient solution comprises the following raw materials in parts by weight: 1.2 to 1.3 portions of magnesium sulfate, 1.1 to 1.2 portions of sodium sulfate, 0.8 to 1.0 portion of zinc sulfate, 0.8 to 0.9 portion of copper sulfate and 0.2 to 0.5 portion of Chinese herbal medicine leaven.
Preferably, the nutrient solution comprises the following raw materials in parts by weight: 1.25 parts of magnesium sulfate, 1.15 parts of sodium sulfate, 0.9 part of zinc sulfate, 0.85 part of copper sulfate and 0.35 part of Chinese herbal medicine leaven.
Preferably, the preparation method of the Chinese herbal medicine starter comprises the steps of adding honeysuckle, wormwood and dandelion into a boiling pot according to the weight ratio of 3:2:1, boiling to obtain juice, feeding the juice and yeast into a fermentation tank together for fermentation treatment, wherein the fermentation temperature is 28-32 ℃, the fermentation time is 1-2 days, and after the fermentation is finished, obtaining the Chinese herbal medicine starter.
Preferably, the preparation method of the modified montmorillonite comprises the steps of adding montmorillonite into deionized water, carrying out dust feeding and ultrasonic dispersion, then drying, grinding for 1-3 times, and then carrying out thermal activation treatment to obtain the modified montmorillonite.
Preferably, the heat activation treatment comprises the following specific steps: raising the temperature of montmorillonite to 150 deg.C at a speed of 2-5 deg.C/min, maintaining for 10min, then continuing to raise the temperature to 210 deg.C at a speed of 1 deg.C/min, continuing to maintain for 20-30min, and naturally returning to room temperature after the heat preservation is finished.
The invention also provides a method for preparing the culture medium for the germ cell dedifferentiation, which comprises the following steps:
step one, weighing the following raw materials in parts by weight:
step two, sequentially adding MS, modified agar, glucose, 2, 4-dichlorophenoxyacetic acid, fructose and nutrient solution into a stirrer, starting the stirrer, stirring for 10-20min at the stirring speed of 700-;
and step three, adding the nutrient solution and the modified montmorillonite into the substrate A, continuing stirring at the low rotation speed of 100-200r/min for 2-5h, wherein the stirring temperature is 35-45 ℃, and after the stirring is finished, obtaining the germ cell dedifferentiation culture substrate.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the germ cell dedifferentiation culture medium, agar is modified by a sodium molybdate solution on a conventional basis, so that the nutrient medium energy of the germ cell dedifferentiation culture medium can be enhanced, the antibacterial environment of the medium can be improved, the culture medium can provide various trace elements by adding a nutrient solution, the differentiation degree of the germ cells is improved, and a Chinese herbal medicine leavening agent is added in the form of a nutrient agent, so that plants can be continuously in the sterile environment when absorbing nutrition, and the antibacterial ability is enhanced.
(2) The montmorillonite dispersion liquid can play an antibacterial function, has strong activity after modification treatment, and can improve the dispersion capacity of montmorillonite in matrix, thereby improving the antibacterial capacity.
(3) The culture medium of the embodiment 3 of the invention has strong antibacterial capacity to germ cells, the infection rate of the germ cells of the embodiment 3 is 0.11 percent, while the infection rate of the germ cells of the comparison example 2 is 1.23 percent, and the embodiment 3 has 1.22 percent of reduction compared with the comparison example 2, thereby having obvious reduction effect.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the embryonic cell dedifferentiation culture medium of the embodiment has the following formula: MS, 6g/L of modified agar, 20g/L of glucose, 0.5mg/L of 2, 4-dichlorophenoxyacetic acid, 0.5mg/L of fructose, 0.mg/L of nutrient solution, 5g/L of modified montmorillonite, and pH value of 5.6.
The preparation method of the modified agar in the embodiment comprises the following steps: irradiating agar by ultraviolet for 10min, then blending with sodium molybdate solution for 10min at the blending speed of 200r/min, finishing blending, filtering, and then drying.
The power of the ultraviolet irradiation in this example was 20W.
The nutrient solution comprises the following raw materials in parts by weight: 1.2 parts of magnesium sulfate, 1.1 parts of sodium sulfate, 0.8 part of zinc sulfate, 0.8 part of copper sulfate and 0.2 part of Chinese herbal medicine leaven.
The preparation method of the Chinese herbal medicine starter comprises the steps of adding honeysuckle, wormwood and dandelion into a boiling pot according to the weight ratio of 3:2:1, boiling to obtain juice, feeding the juice and saccharomycetes into a fermentation tank together for fermentation treatment, wherein the fermentation temperature is 28 ℃, the fermentation time is 1d, and the Chinese herbal medicine starter is obtained after the fermentation is finished.
The preparation method of the modified montmorillonite comprises the steps of adding montmorillonite into deionized water, carrying out dust feeding ultrasonic dispersion, drying, grinding for 1 time, and then carrying out thermal activation treatment to obtain the modified montmorillonite.
The specific steps of the thermal activation treatment in this example are: raising the temperature of montmorillonite to 150 ℃ at the speed of 2 ℃/min, preserving the heat for 10min, then continuing to raise the temperature to 210 ℃ at the speed of 1 ℃/min, continuing to preserve the heat for 20min, finishing the heat preservation, and naturally recovering to the room temperature.
The method for preparing the culture medium for the germ cell dedifferentiation of the embodiment comprises the following steps:
step one, weighing the following raw materials in parts by weight:
step two, sequentially adding MS, modified agar, glucose, 2, 4-dichlorophenoxyacetic acid, fructose and nutrient solution into a stirrer, starting the stirrer, and stirring for 10-20min at the stirring speed of 700r/min to obtain a matrix A;
and step three, adding the nutrient solution and the modified montmorillonite into the substrate A, continuously stirring at the low rotation speed of 100r/min for 2 hours, wherein the stirring temperature is 35 ℃, and stirring is finished to obtain the germ cell dedifferentiation culture substrate.
Example 2:
the embryonic cell dedifferentiation culture medium of the embodiment has the following formula: MS, 8g/L of modified agar, 26g/L of glucose, 1.1mg/L of 2, 4-dichlorophenoxyacetic acid, 1.1mg/L of fructose, 0.7mg/L of nutrient solution, 15g/L of modified montmorillonite, and pH value of 5.8.
The preparation method of the modified agar in the embodiment comprises the following steps: irradiating agar by ultraviolet for 20min, then blending with sodium molybdate solution for 20min at the blending speed of 280r/min, finishing blending, filtering, and then drying.
The power of the ultraviolet irradiation in this example was 100W.
The nutrient solution comprises the following raw materials in parts by weight: 1.3 parts of magnesium sulfate, 1.2 parts of sodium sulfate, 1.0 part of zinc sulfate, 0.9 part of copper sulfate and 0.5 part of Chinese herbal medicine leaven.
The preparation method of the Chinese herbal medicine starter comprises the steps of adding honeysuckle, wormwood and dandelion into a boiling pot according to the weight ratio of 3:2:1, boiling to obtain juice, feeding the juice and saccharomycetes into a fermentation tank together for fermentation treatment, wherein the fermentation temperature is 32 ℃, the fermentation time is 2 days, and the Chinese herbal medicine starter is obtained after the fermentation is finished.
The preparation method of the modified montmorillonite comprises the steps of adding montmorillonite into deionized water, carrying out dust feeding ultrasonic dispersion, drying, grinding for 3 times, and then carrying out thermal activation treatment to obtain the modified montmorillonite.
The specific steps of the thermal activation treatment in this example are: raising the temperature of montmorillonite to 150 ℃ at the speed of 5 ℃/min, preserving the heat for 10min, then continuing to raise the temperature to 210 ℃ at the speed of 1 ℃/min, continuing to preserve the heat for 30min, finishing the heat preservation, and naturally recovering to the room temperature.
The method for preparing the culture medium for the germ cell dedifferentiation of the embodiment comprises the following steps:
step one, weighing the following raw materials in parts by weight:
step two, sequentially adding MS, modified agar, glucose, 2, 4-dichlorophenoxyacetic acid, fructose and nutrient solution into a stirrer, starting the stirrer, and stirring for 20min at the stirring speed of 1000r/min to obtain a matrix A;
and step three, adding the nutrient solution and the modified montmorillonite into the substrate A, continuously stirring at a low rotation speed of 200r/min for 5 hours, wherein the stirring temperature is 45 ℃, and after the stirring is finished, obtaining the germ cell dedifferentiation culture substrate.
Example 3:
the embryonic cell dedifferentiation culture medium of the embodiment has the following formula: MS, 7g/L of modified agar, 23g/L of glucose, 0.7mg/L of 2, 4-dichlorophenoxyacetic acid, 0.8mg/L of fructose, 0.35mg/L of nutrient solution, 10g/L of modified montmorillonite, and pH value of 5.7.
The preparation method of the modified agar in the embodiment comprises the following steps: irradiating agar by ultraviolet for 15min, then blending with a sodium molybdate solution for 15min at the blending speed of 240r/min, finishing blending, filtering, and then drying.
The power of the ultraviolet irradiation in this example was 60W.
The nutrient solution comprises the following raw materials in parts by weight: 1.25 parts of magnesium sulfate, 1.15 parts of sodium sulfate, 0.9 part of zinc sulfate, 0.85 part of copper sulfate and 0.35 part of Chinese herbal medicine leaven.
The preparation method of the Chinese herbal medicine starter comprises the steps of adding honeysuckle, wormwood and dandelion into a boiling pot according to the weight ratio of 3:2:1, boiling to obtain juice, feeding the juice and saccharomycetes into a fermentation tank together for fermentation treatment, wherein the fermentation temperature is 30 ℃, the fermentation time is 1.5 days, and the Chinese herbal medicine starter is obtained after the fermentation is finished.
The preparation method of the modified montmorillonite comprises the steps of adding montmorillonite into deionized water, carrying out dust feeding ultrasonic dispersion, drying, grinding for 2 times, and then carrying out thermal activation treatment to obtain the modified montmorillonite.
The specific steps of the thermal activation treatment in this example are: raising the temperature of montmorillonite to 150 ℃ at the speed of 3.5 ℃/min, preserving the heat for 10min, then continuing to raise the temperature to 210 ℃ at the speed of 1 ℃/min, continuing to preserve the heat for 25min, finishing the heat preservation, and naturally recovering to the room temperature.
The method for preparing the culture medium for the germ cell dedifferentiation of the embodiment comprises the following steps:
step one, weighing the following raw materials in parts by weight:
step two, sequentially adding MS, modified agar, glucose, 2, 4-dichlorophenoxyacetic acid, fructose and nutrient solution into a stirrer, starting the stirrer, and stirring for 15min at the stirring speed of 850r/min to obtain a matrix A;
and step three, adding the nutrient solution and the modified montmorillonite into the substrate A, continuing stirring at a low rotation speed of 150r/min for 3.5 hours, wherein the stirring temperature is 40 ℃, and after stirring is finished, obtaining the germ cell dedifferentiation culture substrate.
Comparative example 1:
the materials and preparation process are basically the same as those of the example 3, except that the modified montmorillonite is not added.
Comparative example 2:
the materials and preparation process were substantially the same as those of example 3, except that a conventional culture medium was used.
The results of the culture substrate tests of examples 1 to 3 and comparative examples 1 to 2 are shown in Table 1
| Group of | Bacterial contamination ratio (%) |
| Example 1 | 0.12 |
| Example 2 | 0.14 |
| Example 3 | 0.11 |
| Comparative example 1 | 0.27 |
| Comparative example 2 | 1.23 |
TABLE 1
As can be seen from Table 1, the medium of example 3 of the present invention has a strong antibacterial ability against germ cells, and the germ cell contamination rate of example 3 is 0.11%, while that of comparative example 2 is 1.23%, and example 3 is reduced by 1.22% compared to comparative example 2, which has a significant reduction effect.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (9)
1. The culture medium for the dedifferentiation of the germ cells is characterized by comprising the following components in percentage by weight: MS, 6-8g/L of modified agar, 20-26g/L of glucose, 0.5-1.1mg/L of 2, 4-dichlorophenoxyacetic acid, 0.5-1.1mg/L of fructose, 0.2-0.7mg/L of nutrient solution, 5-15g/L of modified montmorillonite, and pH value of 5.6-5.8.
2. The embryonic bud cell dedifferentiation culture medium according to claim 1, wherein the modified agar is prepared by the method comprising: irradiating the agar by ultraviolet for 10-20min, then blending the agar and a sodium molybdate solution for 10-20min at the blending rotation speed of 200-280r/min, filtering after blending, and then drying.
3. The culture medium for dedifferentiating embryonic cells according to claim 2, characterized in that the power of the UV radiation is 20-100W.
4. The culture medium for dedifferentiation of germ cells according to claim 1, wherein the nutrient solution comprises the following raw materials in parts by weight: 1.2 to 1.3 portions of magnesium sulfate, 1.1 to 1.2 portions of sodium sulfate, 0.8 to 1.0 portion of zinc sulfate, 0.8 to 0.9 portion of copper sulfate and 0.2 to 0.5 portion of Chinese herbal medicine leaven.
5. The culture medium for dedifferentiation of germ cells according to claim 4, wherein the nutrient solution comprises the following raw materials in parts by weight: 1.25 parts of magnesium sulfate, 1.15 parts of sodium sulfate, 0.9 part of zinc sulfate, 0.85 part of copper sulfate and 0.35 part of Chinese herbal medicine leaven.
6. The culture medium for the dedifferentiation of germ cells according to claim 5, wherein the Chinese herbal medicine starter culture is prepared by adding honeysuckle, wormwood and dandelion in a boiling pot according to a weight ratio of 3:2:1 to boil, feeding the obtained juice and yeast into a fermentation tank together to perform fermentation treatment, wherein the fermentation temperature is 28-32 ℃, the fermentation time is 1-2 days, and the Chinese herbal medicine starter culture is obtained after the fermentation is finished.
7. The embryonic germ dedifferentiation culture medium according to claim 1, wherein the modified montmorillonite is prepared by adding montmorillonite into deionized water, performing ultrasonic dispersion on the mixture, drying the mixture, grinding the mixture for 1 to 3 times, and performing thermal activation treatment on the ground mixture to obtain the modified montmorillonite.
8. The embryonic cell dedifferentiation culture medium according to claim 7, wherein the heat activation treatment comprises the following specific steps: raising the temperature of montmorillonite to 150 deg.C at a speed of 2-5 deg.C/min, maintaining for 10min, then continuing to raise the temperature to 210 deg.C at a speed of 1 deg.C/min, continuing to maintain for 20-30min, and naturally returning to room temperature after the heat preservation is finished.
9. A method for preparing a culture medium for the dedifferentiation of germ cells according to any one of claims 1 to 8, comprising the following steps:
step one, weighing the following raw materials in parts by weight:
step two, sequentially adding MS, modified agar, glucose, 2, 4-dichlorophenoxyacetic acid, fructose and nutrient solution into a stirrer, starting the stirrer, stirring for 10-20min at the stirring speed of 700-;
and step three, adding the nutrient solution and the modified montmorillonite into the substrate A, continuing stirring at the low rotation speed of 100-200r/min for 2-5h, wherein the stirring temperature is 35-45 ℃, and after the stirring is finished, obtaining the germ cell dedifferentiation culture substrate.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1962853A (en) * | 2006-11-23 | 2007-05-16 | 上海光兆植物速生技术有限公司 | Method for building poplar embryogenic cell generation technology system |
| CN101168732A (en) * | 2007-08-24 | 2008-04-30 | 清华大学 | Method for producing Vinca rosea alkaloid |
| CN101371650A (en) * | 2007-08-24 | 2009-02-25 | 中国农业科学院蔬菜花卉研究所 | Method for inducing dissociate microspore callus of eggplant and regenerating plant strain |
| CN101946703A (en) * | 2010-08-17 | 2011-01-19 | 华中农业大学 | Method for regenerating plants of Chinese rose by using leaves as explants |
| CN102524082A (en) * | 2012-03-17 | 2012-07-04 | 常熟市尚湖农业生态园有限公司 | Method of nanometer tissue culture of iris ensata |
| CN104126505A (en) * | 2014-07-07 | 2014-11-05 | 沈阳农业大学 | Somatic embryo in-vitro regeneration method applied to genetic transformation and seed ball rapid propagation of Lilium pumilumDC. Fisch |
| CN104719158A (en) * | 2015-03-09 | 2015-06-24 | 中国农业科学院兰州畜牧与兽药研究所 | Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants |
| CN106106162A (en) * | 2016-07-11 | 2016-11-16 | 武汉生物工程学院 | A kind of oolong head rapid propagation method |
| CN106258976A (en) * | 2016-08-24 | 2017-01-04 | 华中农业大学 | A kind of tissue culturing fast seedling-cultivating method of mustard type rape |
| CN108575758A (en) * | 2018-05-28 | 2018-09-28 | 杭州雪域生物技术有限公司 | A kind of culture media composition of Herba Saussureae Involueratae tissue cultures and its application |
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
| CN109644869A (en) * | 2018-12-20 | 2019-04-19 | 长沙学院 | The method for obtaining Gardenoside by tissue cultures |
-
2019
- 2019-11-22 CN CN201911158172.0A patent/CN110845266A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1962853A (en) * | 2006-11-23 | 2007-05-16 | 上海光兆植物速生技术有限公司 | Method for building poplar embryogenic cell generation technology system |
| CN101168732A (en) * | 2007-08-24 | 2008-04-30 | 清华大学 | Method for producing Vinca rosea alkaloid |
| CN101371650A (en) * | 2007-08-24 | 2009-02-25 | 中国农业科学院蔬菜花卉研究所 | Method for inducing dissociate microspore callus of eggplant and regenerating plant strain |
| CN101946703A (en) * | 2010-08-17 | 2011-01-19 | 华中农业大学 | Method for regenerating plants of Chinese rose by using leaves as explants |
| CN102524082A (en) * | 2012-03-17 | 2012-07-04 | 常熟市尚湖农业生态园有限公司 | Method of nanometer tissue culture of iris ensata |
| CN104126505A (en) * | 2014-07-07 | 2014-11-05 | 沈阳农业大学 | Somatic embryo in-vitro regeneration method applied to genetic transformation and seed ball rapid propagation of Lilium pumilumDC. Fisch |
| CN104719158A (en) * | 2015-03-09 | 2015-06-24 | 中国农业科学院兰州畜牧与兽药研究所 | Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants |
| CN106106162A (en) * | 2016-07-11 | 2016-11-16 | 武汉生物工程学院 | A kind of oolong head rapid propagation method |
| CN106258976A (en) * | 2016-08-24 | 2017-01-04 | 华中农业大学 | A kind of tissue culturing fast seedling-cultivating method of mustard type rape |
| CN108575758A (en) * | 2018-05-28 | 2018-09-28 | 杭州雪域生物技术有限公司 | A kind of culture media composition of Herba Saussureae Involueratae tissue cultures and its application |
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
| CN109644869A (en) * | 2018-12-20 | 2019-04-19 | 长沙学院 | The method for obtaining Gardenoside by tissue cultures |
Non-Patent Citations (2)
| Title |
|---|
| 王炜等: "小麦花药培养的研究和应用", 《核农学报》 * |
| 齐海军等: "均匀设计法优化人参叶片愈伤组织诱导培养基", 《北方园艺》 * |
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Application publication date: 20200228 |