KR101402077B1 - A compound mushroom powder process using grain medium and whole soybean curd process using compound mushroom powder - Google Patents
A compound mushroom powder process using grain medium and whole soybean curd process using compound mushroom powder Download PDFInfo
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- A23C20/025—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
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Abstract
Description
본 발명은 2종 이상의 버섯을 발효곡물 배지에 배양한 후 건조 및 분쇄시켜 건강식으로 사용하는 한편, 완성된 버섯분말은 전두부의 제조에 사용하여 건강을 증진시킬 수 있는 고기능성의 식용제품을 제조할 수 있도록 한 발명에 관한 것이다.
In the present invention, two or more kinds of mushrooms are cultured in a fermented grain medium, dried and crushed to be used in a healthful manner, and the finished mushroom powder is used in the production of a frontal part to produce a high-function food product capable of promoting health The present invention relates to the invention.
일반적으로 버섯(mushroom)은 독특한 향기와 맛을 갖고 있기 때문에 세계 각지에서 널리 애용되는 식품으로 버섯에는 다양한 효소가 포함되어 면역력을 증강시키고 혈액 순환을 개선시키며 면역력이 있는 세포를 활성화시켜 항암 효과가 있는 것으로 알려져 있으며 학회에도 보고되고 있다.Generally, mushroom is a widely used food in many parts of the world because it has a unique aroma and taste. It contains various enzymes to enhance immunity, improve blood circulation, activate immunocompetent cells, And has been reported to the Society.
버섯은 자연에서 채취하는 것이 어렵고 수확량이 적기 때문에 비닐하우스와 같은 온실에서 톱밥이나 나무를 이용하여 버섯 자실체(字實體)를 재배하는 것이 일반적이다.Mushrooms are difficult to harvest from nature and harvest less, so it is common to grow mushroom fruiting bodies in sawmills such as plastic houses using sawdust or wood.
그러나, 온실과 같은 설비를 이용하여 버섯을 재배하는 경우에는 생산시설의 투자에 막대한 비용이 소요되는 등의 문제점이 발생되었고, 버섯의 재배에도 상당한 기간이 소요되어 충분한 양을 적기에 공급할 수 없었다.However, when mushrooms were grown using a facility such as a greenhouse, it took a considerable amount of time to invest in production facilities, and it took a considerable period of time to cultivate the mushroom, so that it was not possible to supply sufficient amounts in time.
특히, 단순하게 톱밥이나 나무에 종균(種菌)을 접종하여 버섯을 재배하는 경우에는 효능이 우수한 고품질의 버섯을 재배할 수 없었으므로 버섯을 배양하기 위한 배지의 특성을 높여 고기능성의 버섯을 재배할 수 있는 기술이 요구되었다.In particular, when mushrooms are grown by simply inoculating seeds or seeds into sawdust or trees, high-quality mushrooms having excellent efficacy can not be grown. Therefore, the characteristics of the medium for cultivating mushrooms are raised, and highly functional mushrooms are grown Technology was required.
더욱이 버섯을 단순하게 재배하는 경우에는 버섯이 갖는 각각의 효능 외에는 보다 특이하고 우수한 효능을 기대할 수 없었으므로 각각의 버섯들이 갖는 효능들이 상충되어 보다 우수한 효능을 갖도록 하여 항암 효과 및 체내 면역력을 높여줄 수 있는 고기능성의 버섯분말 제조방법을 제안하게 되었다.
In addition, when mushrooms are simply cultivated, the mushrooms can not be expected to have more specific and superior efficacy than the respective effects, so that the efficacies of the respective mushrooms are in conflict with each other so that they have better efficacy and can enhance the anti-cancer effect and the immune system And a method for producing the highly functional mushroom powder.
본 발명은 상기한 문제점을 감안하여 창안한 것으로서, 그 목적은 곡물을 발효시킨 특수 배지에 2종 이상의 버섯을 배양하여 건조 및 분쇄시켜 각각의 버섯들이 갖는 효능들이 상충되어 보다 우수한 효능을 갖는 복합버섯분말을 제공하는 한편, 완성된 버섯분말은 전두부의 제조에 사용하여 건강을 증진시킬 수 있는 고기능성의 발효곡물 배지를 이용한 복합버섯분말의 제조방법 및 복합버섯분말을 이용한 전두부의 제조방법을 제공함에 있는 것이다.
DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a mushroom culture method in which at least two kinds of mushrooms are cultivated in a special medium fermented with cereals and dried and crushed, The present invention also provides a method for preparing a composite mushroom powder using a highly functional fermented grain medium that can be used for manufacturing a frontal part and improving health, and a method for manufacturing a frontal part using a composite mushroom powder It is.
상기한 목적을 달성하기 위한 본 발명의 특징은, 곡물을 복합미생물 발효액에 침지(浸漬)하여 22∼45℃의 온도로 2∼5시간 발효시키는 단계; 발효된 곡물을 탈수하여 수분함유량이 30∼50%가 유지되는 곡물배지를 형성하는 단계; 곡물배지를 살균된 배양용기에 넣고 송이버섯, 차가버섯, 표고버섯, 상황버섯, 잎새버섯, 노루궁뎅이버섯, 영지버섯들 중에서 선택된 2종이상의 종균(種菌)을 곡물배지에 접종하는 단계; 배양용기의 마개를 닫고 24∼30℃의 온도를 유지하는 배양실에서 9∼12일간 배양시키는 단계; 2종류 이상의 버섯이 돋아난 곡물배지를 멸균기에 투입하여 1∼2기압(氣壓) 및 120∼130℃의 온도를 부여하여 10∼20분간 멸균시키는 단계; 멸균이 완료된 버섯과 곡물배지를 45∼55℃의 온도를 부여하여 4∼6시간 건조시키는 단계; 건조가 완료된 버섯과 곡물배지를 함께 분쇄하여 100∼300메시의 입도(粒度)를 갖는 체로 걸러서 버섯분말을 완성하는 단계로 이루어진 것을 특징으로 하는 발효곡물 배지를 이용한 복합버섯분말의 제조방법에 의하여 달성될 수 있는 것이다.
According to an aspect of the present invention, there is provided a method for producing a fermented microorganism, which comprises: immersing a grain in a fermentation broth of a microorganism and fermenting the fermented broth at a temperature of 22 to 45 ° C for 2 to 5 hours; Dehydrating the fermented cereal to form a cereal medium in which the moisture content is maintained at 30 to 50%; A step of putting the grain medium into a sterilized culture container and inoculating two or more kinds of seeds selected from pine mushroom, mushroom, shiitake mushroom, mushroom, mushroom, mushroom, and myrtle into a grain medium; Incubating the culture vessel for 9 to 12 days in a culture room in which the temperature is maintained at 24 to 30 DEG C by closing the cap of the culture vessel; Adding at least two types of mushroom-grown cereal medium to a sterilizer, sterilizing the mixture at a temperature of 1 to 2 atmospheres and a temperature of 120 to 130 DEG C for 10 to 20 minutes; Drying the sterilized mushroom and the cereal medium at a temperature of 45 to 55 캜 for 4 to 6 hours; And a step of pulverizing the dried mushroom and the cereal medium together and filtering the dried mushroom with a sieve having a particle size of 100 to 300 mesh to complete the mushroom powder. According to the method for producing the mushroom powder using the fermented grain medium, It can be.
이상에서 상술한 바와 같은 제조과정에 의하여 완성된 복합버섯분말은 복합미생물 발효액으로 곡물을 발효시킨 배지(培地)를 사용하여 버섯을 배양시킨 것이므로 각종 효소들이 풍부하게 함유되어 우수한 고기능성·고효능을 갖는 고품질의 식용 버섯분말을 제공할 수 있는 등의 이점이 있는 것이다.
As described above, the composite mushroom powder obtained by the above-described manufacturing process is cultivated with mushroom using a medium in which cereal is fermented with a complex microbial fermentation liquid, so that it is rich in various enzymes and has excellent high functionality and high efficacy It is possible to provide a high-quality edible mushroom powder having the above properties.
도 1은 본 발명에 의한 복합버섯분말의 제조과정을 순차적으로 나열한 제조공정도.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a manufacturing process diagram sequentially illustrating the manufacturing process of composite mushroom powder according to the present invention; FIG.
이하, 상기한 목적을 달성하기 위한 바람직한 실시예를 첨부된 도 1의 제조 공정도에 의하여 상세히 설명하면 다음과 같다.DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
실시예Example
곡물을 복합미생물 발효액에 담그고 22∼45℃의 온도를 유지하는 발효실에 투입하여 2∼5시간 발효시키는 단계를 실시하였다.The grains were immersed in the mixed microbial fermentation broth and fed to a fermentation chamber maintained at a temperature of 22 to 45 캜 for fermentation for 2 to 5 hours.
상기 복합미생물 발효액에 침지(浸漬)되는 곡물은 콩, 팥, 수수, 기장, 차, 조, 율무, 현미, 보리, 흑미, 밀, 녹두 중에서 어느 하나이거 나 이들의 조합으로 구성된 군에서 선택된 것을 사용하였다.The grains to be immersed in the complex microorganism fermentation broth are selected from the group consisting of soybean, red bean, sorghum, millet, tea, jojoba, brown rice, barley, black rice, wheat and mung bean or a combination thereof Respectively.
이어서, 발효된 곡물을 탈수하여 물기를 제거하고, 수분함유량이 30∼50%가 유지되도록 하여 곡물배지(穀物培地)를 형성하는 단계를 실시하였다.Then, the fermented grain was dehydrated to remove water, and a moisture content of 30 to 50% was maintained to form a grain medium (cereal medium).
이어서, 곡물배지를 살균된 배양용기에 넣고 송이버섯, 차가버섯, 표고버섯, 상황버섯, 잎새버섯, 노루궁뎅이버섯, 영지버섯들 중에서 선택된 2종이상의 종균(種菌)을 곡물배지에 접종하는 단계를 실시하였다.Next, the grain medium is placed in a sterilized culture container and a step of inoculating two kinds of seeds selected from matsutake mushrooms, mushrooms, shiitake mushrooms, mushrooms, mushrooms, mushrooms, and myrrh Respectively.
상기 종균은 곡물의 종류에 따라 차이가 있으며, 곡물에 대해 0,01∼0.1중량%를 접종하였다.The seeds differ depending on the kind of grains and are inoculated with 0,01-0.1 wt% of the grains.
이어서, 배양용기의 마개를 닫고 24∼30℃의 온도를 유지하는 배양실에 투입하여 9∼12일간 버섯을 배양시키는 단계를 실시하였다.Then, the cap of the culture container was closed, and the culture medium was kept at a temperature of 24 to 30 DEG C to carry out a step of culturing the mushroom for 9 to 12 days.
상기 과정에서 배양된 버섯은 곡물배지에서 3∼7mm 크기로 자란 자실체(字實體)를 형성하였고, 본 발명에서는 곡물배지와 자실체를 함께 분쇄하여 사용한다.The mushroom cultivated in the above process forms a fruiting body grown in a size of 3 to 7 mm in the grain medium. In the present invention, the grain medium and the fruiting body are pulverized together.
이어서, 2종류 이상의 버섯이 돋아난 곡물배지를 멸균기에 투입하여 10∼20분간 멸균시키는 단계를 실시하였다.Then, a step of putting two or more types of mushroom-grown cereal medium into a sterilizer and sterilizing for 10 to 20 minutes was performed.
이때 멸균기의 내부 온도는 120∼130℃가 유지되도록 하고, 내부 기압은 1∼2기압(氣壓)이 유지되는 고압멸균기(高壓滅菌器)를 사용하였다. 상기 고압멸균기는 고압에 충분히 견딜 수 있는 금속 케이스의 내부에 약 120℃의 증기를 분무하면서 가열하고 압력을 가하여 멸균하는 압력장치를 사용하였다.At this time, the internal temperature of the sterilizer was maintained at 120 to 130 ° C, and a high pressure sterilizer was used in which the internal pressure was maintained at 1 to 2 atmospheric pressure. In the high-pressure sterilizer, a pressure device for sterilizing the inside of a metal case capable of withstanding high pressure by heating and pressurizing steam of about 120 ° C was used.
이어서, 멸균이 완료된 버섯과 곡물배지를 건조실에 투입하여 45∼55℃의 온도를 부여하면서 4∼6시간 건조시키는 단계를 실시하였다.Then, the sterilized mushroom and the cereal medium were put into a drying chamber and dried at a temperature of 45 to 55 캜 for 4 to 6 hours.
이어서, 건조가 완료된 버섯과 곡물배지를 함께 분쇄한 후 100∼300메시(mesh)의 입도(粒度)를 갖는 체로 걸러서 고운 입자를 갖는 복합버섯분말을 완성하였다.Then, the dried mushroom and the cereal medium were pulverized together and then sieved with a particle having a particle size of 100 to 300 mesh to complete a composite mushroom powder having fine particles.
전술한 제조과정에 의하여 완성된 복합버섯분말은 복합미생물 발효액으로 곡물을 발효시킨 배지(培地)를 사용하여 버섯을 배양시킨 것이므로 각종 효소들이 풍부하게 함유되어 버섯분말을 식용으로 섭취하였을 때 체내 효소작용을 활성화시켜 소화흡수력을 높여줄 수 있고, 항산화효과를 얻을 수 있는 동시에 2종류 이상의 버섯 종균을 배양한 것이므로 각각의 버섯들이 배양과정에서 종균들이 서로 간섭을 일으키며 증식되어 부족한 성분을 보충하고 유익한 효능들은 서로 상충되어 보다 면역력이 우수한 고기능성·고효능을 갖는 고품질의 식용 버섯분말을 제공할 수 있는 등의 이점이 있는 것이다.The complex mushroom powder obtained by the above-mentioned manufacturing process is a mixture culture of mushroom using a medium in which cereal is fermented with a complex microbial fermentation broth. Therefore, when the mushroom powder is ingested for food, It is possible to increase digestion absorption power and to obtain antioxidant effect and at the same time to cultivate two or more kinds of mushroom seeds, each mushroom interferes with each other during culturing and replenishes insufficient components, and beneficial effects It is possible to provide a high-quality edible mushroom powder having high functionality and high efficacy, which is superior in immunity and in conflict with each other.
또한, 발효된 곡물배지와 3∼7mm 크기로 자란 자실체(字實體)를 함께 분쇄하여 식용 버섯분말을 형성한 것이므로 발효곡물의 항산화효능 및 복합버섯의 면역력 증대효과들이 어우러진 고기능성의 식용분말을 제조하여 각종 식품에 첨가하여 사용할 수 있는 것이다.In addition, since the fermented grain medium and fruiting bodies grown in the size of 3 to 7 mm are crushed together to form edible mushroom powder, a high-functional edible powder is produced which combines the antioxidant effect of the fermented grain and the immunity enhancement effects of the compound mushroom It can be added to various foods and used.
한편, 상기 곡물의 발효에 사용되는 복합미생물 발효액의 제조과정은 다음과 같다.The process for producing the complex microbial fermentation liquid used for the fermentation of the cereal is as follows.
즉, 생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 가하고 유산균을 부여하여 발효시킨 다음 가압 증류하여 획득한 추출물을 숙성 발효시키는 1 차 유산균 발효단계, 1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 발효시키는 2차 유산균 발효단계, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 발효시키는 3차 효모균 발효단계, 3차 효모균 발효물에 방선균·사상균·고초균·황국균·초산균을 접종하고 발효시키는 4차 발효단계, 4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 발효시키는 5차 발효단계에 의하여 복합미생물 발효액이 완성된다.That is, it is preferable that the fermentation step is a primary lactic acid fermentation step in which 0.3 to 0.8 wt% of salt is added to the total weight of the herbal medicines, the fermented lactic acid bacterium is added and fermented, A step of fermenting a second lactic acid bacterium to which a water soluble propolis is added in an amount of 0.5 to 2% by weight based on the fermented product of the second lactic acid bacterium, A fourth fermentation step of inoculating and fermenting actinomycetes, filamentous fungi, kojuco, hwangguk gum, and acetic acid bacteria to yeast fermented product, adding 0.05 to 1 wt% of glycerin to the fourth fermented product, homogenizing at a high speed and dispersing, The complex microbial fermentation broth is completed.
상기 미생물 발효액 제조과정의 실시예는 다음과 같다.Examples of the microbial fermentation broth manufacturing process are as follows.
생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 첨가하고 미량의 유산균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시킨 다음 가압 증류하여 추출물을 획득하고, 온도 30∼40℃의 온도를 유지하는 발효조에서 7∼10 일간 숙성 발효시키는 1차 유산균 발효단계를 실시하였다.0.3 to 0.8% by weight of salt was added to the total weight of the herbal medicine, and a small amount of lactic acid bacteria was added thereto. The fermented product was fermented in a fermenter maintained at a temperature of 30 to 40 캜 for about 2 days and then subjected to pressure distillation to obtain an extract. Fermentation step of fermenting primary fermented lactic acid fermented for 7 to 10 days in a fermenter maintained at a temperature of 40 ° C.
상기 "생약재"란 천연에서 얻어지는 의약품으로서, 참나무잎, 두송잎, 진피, 갈근, 갈화, 갈엽, 계피, 송순, 약쑥, 헛개나무 열매, 겨우살이, 조릿대, 까마중, 민들레, 인동꽃, 구기자, 및 이들의 조합으로 구성된 군에서 선택된 생약재를 사용하였다.The term "herbal medicine" means a medicament obtained from a natural source, such as an oak leaf, a leaf, a dandelion, a pupa, a crab, a lobule, a cinnamon, a songson, a wormwood, a hovenous fruit, a mistletoe, Was used as the herbal medicine.
상기 유산균은 락토바실러스 루테리, 락토바실러스 카제이, 락토바실러플란타룸, 락토바실러스 애시도필러스 및 이들의 조합을 사용하였다.The lactic acid bacteria used were Lactobacillus luteri, Lactobacillus casei, Lactobacillus lafflata, Lactobacillus acidophilus, and combinations thereof.
상기 가압증류는 증류장치에 대기압보다 높은 압력을 가하고 진행하는 증류 방법을 사용하였다.The distillation method was used in which the pressure distillation was performed by applying a pressure higher than atmospheric pressure to the distillation apparatus.
이어서, 1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 2차 유산균 발효단계를 실시하였다.Next, a fermentation step of a second lactic acid bacterium was carried out by adding 0.1 to 0.5% by weight of water-soluble propolis to the fermented product of the first lactic acid bacteria, adding lactic acid bacteria, and fermenting the fermented product in a fermenter maintained at a temperature of 30 to 40 ° C for about 2 days.
상기 수용성 프로폴리스는 그 자체가 항산화 효과를 갖는 물질이지만, 본 발명에 따른 상기 미생물 발효액은 프로폴리스의 단순한 부가에 의해 얻어지는 항산화 효과보다 현저히 높은 항산화 효과가 얻어질 수 있다.The water-soluble propolis itself is a substance having an antioxidative effect, but the microbial fermentation broth according to the present invention can achieve a significantly higher antioxidative effect than the antioxidative effect obtained by the simple addition of propolis.
이어서, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 3차 효모균 발효단계를 실시하였다.Next, a fermentation step of the third yeast strain, in which 0.5-2 wt% of minerals were added to the fermented secondary lactic acid bacteria and yeast strains were added thereto and fermented in a fermenter maintained at a temperature of 30-40 ° C for about 2 days, was performed.
상기 "미네랄"은 인체의 성장과 유지 및 생식에 비교적 소량 필요한 영양물질인 광물질을 사용하였다.The "minerals" used minerals that are nutrients that are relatively small in quantity for growth, maintenance and reproduction of the human body.
상기 효모균은 통상의 효모균 발효에 사용될 수 있는 임의의 효모균이 사용될 수 있으며 사카로마이세스 세레비지애(Saccharomyces cerevisiae), 사카로아미세스 엘리소이더스(Saccharomyces ellipsoidus) 또는 이들의 조합을 사용하였다.The yeast may be any yeast that can be used for conventional yeast fermentation, and Saccharomyces cerevisiae, Saccharomyces ellipsoidus, or a combination thereof is used.
이어서, 3차 효모균 발효물에 방선균·사상균·고초균·황국균 및 초산균을 접종하고 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 4차 발효단계를 실시하였다.Next, a fourth fermentation step in which the actinomycetes, filamentous fungi, Bacillus subtilis, Hwang gukguk and acetic acid bacteria were inoculated into the fermentation product of the third yeast strain and fermented in a fermenter kept at a temperature of 30 to 40 캜 for about 2 days was carried out.
상기 방선균은 스트렙토마이세스 그리세우스(Streptomyces griseus), 스트렙토마이세스 아우레우스(Streptomyces aureus), 스트렙토마이세스 아비도플라부스(Streptomycesabisoflavus) 또는 이들의 조합을 사용하였다.The actinomycetes used Streptomyces griseus, Streptomyces aureus, Streptomyces abisoflavus, or a combination thereof.
상기 사상균은 누룩곰팡이속 (Aspergillus), 페니실리움속(Penicillium), 리조푸스속(Rhizopus)이 만들어낸 가는 실 모양의 균사체(菌絲體)와 자실체(子實體)를 사용하였다.The filamentous fungi used were filamentous fungi and fruiting bodies produced by Aspergillus, Penicillium, and Rhizopus.
상기 고초균은 바실러스 서브틸리스(Bacillus subtilis), 바실러스 서브틸리스 스피지제피아이(Bacillus subtilis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 리체니포르미스(Bacillus licheniformis), 바실러스 풀미스(Bacillus pumilus) 또는 이들의 조합을 사용하였다.The Bacillus subtilis may be selected from the group consisting of Bacillus subtilis, Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, Bacillus pumilus, ) Or a combination thereof.
이어서, 4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 10∼30℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 5차 발효단계를 실시하여 복합 미생물 발효액을 완성하였다.Then, 0.05 to 1% by weight of glycerin was added to the fourth fermentation product, and homogenized at high speed and dispersed. Then, the fermentation was carried out in a fermenter maintained at a temperature of 10 to 30 캜 for about 2 days, .
상기 "글리세린"은 화학식 C3H5(OH)3, 분자량 92.10으로 흡습성이 강하고, 수용성이며, 자당의 3분의 1 정도의 감미(甘味)가 있는 식품 첨가제이다.The above-mentioned "glycerin" is a food additive having the formula C 3 H 5 (OH) 3 , a molecular weight of 92.10 and being highly hygroscopic, water-soluble and having about one-third of sucrose.
상기 고속분산 작업은 4차 복합 발효물과 글리세린의 혼합물을 균질화시키기 위해 수행하는 것으로서 800∼1000rpm의 속도로 회전시켜 균질화하였다.The high-speed dispersing operation was performed to homogenize a mixture of the fourth composite fermented product and glycerin, and was homogenized by rotating at a speed of 800 to 1000 rpm.
상기 5차 발효에 의하여 완성된 미생물 발효액은 그 자체로서 항산화 효과가 매우 뛰어날 뿐만 아니라 섭취하였을 때 체내 효소작용에 도움을 주며, 음식을 섭취 후 음용하면 소화 흡수를 촉진시키고, 일반적인 장내 부패균의 병원성 세균의 증식을 억제하는 효과가 있다.The fermentation broth of the microorganism completed by the fifth fermentation is not only excellent in antioxidative effect itself but also contributes to the enzymatic action when ingested and promotes digestion and absorption by ingesting food after ingestion, The effect of suppressing the proliferation of the cells.
한편, 상기 제조과정에 의하여 완성된 복합 버섯가루를 사용하여 전두부를 제조하는 과정은 다음과 같다.Meanwhile, the process of manufacturing the front head using the compound mushroom powder completed by the above-mentioned manufacturing process is as follows.
콩의 껍질을 벗기고 분쇄한 콩가루 66∼70중량%, 복합 버섯가루 27∼31중량%, 야채가루 2∼4중량%를 고르게 혼합하는 단계; 고르게 섞여진 가루분말을 물과 혼합하여 두유(豆乳)를 만들고, 가열용기에 두유를 넣고 110∼120℃의 온도로 가열하여 증숙하는 단계; 가열된 두유를 식힌 후 천연재료 응고제를 투입하고 고르게 저어 응고시키는 단계; 응고된 재료를 포장용기에 투입하고 밀봉하여 두부의 형태를 유지한 상태에서 완전히 응고되어 전두부가 완성되는 단계로 구성되어 있다.66 to 70% by weight of soybean flour peeled and ground, 27 to 31% by weight of compound mushroom powder, and 2 to 4% by weight of vegetable powder; Mixing soymilk powder with water to make soymilk, adding soymilk to a heating vessel, and heating the mixture at a temperature of 110 to 120 캜; Cooling the heated soymilk, adding a natural material coagulant, and stirring and evenly mixing the natural coagulant; The coagulated material is put into a packaging container and sealed to form a completely frozen state with the shape of the tofu being maintained, thereby completing the frontal part.
이하, 전두부의 제조방법 실시예는 다음과 같다.Hereinafter, examples of the method of manufacturing the front head portion are as follows.
콩(大豆)의 껍질을 벗긴 후 200∼300메시의 입도(粒度)를 갖도록 분쇄한 콩가루 66∼70중량%, 복합 버섯가루 27∼31중량%, 야채가루 2∼4중량%를 고르게 혼합하는 단계를 실시하였다.A step of uniformly mixing 66 to 70 wt% of soybean flour ground, 27 to 31 wt% of compound mushroom powder, and 2 to 4 wt% of vegetable powder so as to have a grain size of 200 to 300 mesh after peeling the bean (soybean) Respectively.
상기 야채가루는 백년초, 당근, 치자, 시금치, 클로렐라(chlorella) 중 어느 하나이거나 이들의 조합으로 구성된 군에서 선택된 것을 건조시킨 후 200∼300메시의 입도(粒度)를 갖도록 분쇄한 것을 사용하였다.The vegetable powder was selected from the group consisting of one hundred years of fresh water, carrot, gardenia, spinach, chlorella, or a combination thereof, and then dried to obtain a powder having a particle size of 200 to 300 mesh.
이어서, 고르게 섞여진 가루분말을 물과 혼합하여 두유(豆乳)를 만들고, 가열용기에 두유를 넣고 110∼120℃의 온도로 가열하여 증숙하는 단계를 실시하였다.Then, powder of the powder mixed evenly was mixed with water to make soymilk, soy milk was added to the heating vessel, and the mixture was heated at a temperature of 110 to 120 캜 for boiling.
이때 가열용기의 내부 기압은 0.7∼1기압이 되도록 하여 10∼20분간 가열하였다.At this time, the internal pressure of the heating vessel was heated to 10 to 20 minutes so that the pressure was 0.7 to 1 atm.
이어서, 가열된 두유를 식힌 후 천연재료 응고제를 투입하고 고르게 저어 응고시키는 단계를 실시하였다.Then, the heated soymilk was cooled, and then a natural material coagulant was added thereto, followed by uniform stirring and coagulation.
상기 두유의 냉각(冷却)은 상온에 방치하여 서서히 냉각시키면서 두유에 남아 있는 미열을 제거하여 지속적인 열변성이 발생되지 않도록 하였다.The cooling (cooling) of the soybean milk was allowed to proceed at room temperature and gradually cooled to remove the remaining heat in the soybean milk to prevent continuous heat denaturation.
상기 천연재료 응고제는 구기자, 복분자, 오미자, 함초, 신선초 중 어느 하나이거나 이들의 조합으로 구성된 군에서 선택한 것을 사용하였고, 천연재료를 분쇄하고, 유산균으로 6∼8일 발효시킨 후 두유에 대해 0.8∼2중량% 첨가하여 사용하였다. Wherein the natural material coagulant is selected from the group consisting of gugija, bokbunja, omija, green tea, ginseng, or a combination thereof. The natural material is pulverized, fermented with lactic acid bacteria for 6-8 days, 2% by weight.
이어서, 응고된 재료를 포장용기에 투입하고 8∼10℃에서 냉각하여 두부의 형태를 유지한 상태에서 완전히 응고된 상태에서 밀봉하여 전두부를 완성하였다.Subsequently, the solidified material was put into a packaging container and cooled at 8 to 10 DEG C to complete the head portion while maintaining the shape of the head portion in a completely solidified state.
전술한 제조과정에 의하여 완성된 전두부는 복합 버섯가루를 사용하여 다양한 효소가 풍부한 고기능성·고품질의 전두부를 제공할 수 있는 동시에 콩 껍질을 벗기고 그대로 응고하여 성형한 것이므로 외관이 매끈하면서도 두유의 영양소를 전부 함유하고 있는 최고의 영양식을 구현할 수 있을 뿐 아니라 비지의 발생이 없고 가공과정에서 폐수가 발생되지 않아 환경오염을 유발하지 않는 친화경적인 제조방법으로 전두부를 제조할 수 있는 것이다.The frontal part completed by the above-mentioned manufacturing process can provide a high-functional, high-quality frontal part rich in various enzymes by using the compound mushroom powder, and at the same time, the bean husk is peeled off and solidified as it is. It is possible to manufacture the frontal part by using an affinity manufacturing method which not only can realize the best nutrition formula containing all but also does not cause scum and does not generate wastewater during processing and does not cause environmental pollution.
이상에서는 본 발명의 바람직한 실시예에 대하여 도시하고 또한 설명하였으나, 본 발명은 상기한 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 본 발명이 속하는 분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형 실시가 가능한 것은 물론이고, 그와 같은 변경은 기재된 청구범위 내에 있게 된다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the invention. It will be understood by those skilled in the art that various changes may be made and equivalents may be resorted to without departing from the scope of the appended claims.
Claims (4)
발효된 곡물을 탈수하여 수분함유량이 30∼50%가 유지되는 곡물배지를 형성하는 단계;
곡물배지를 살균된 배양용기에 넣고 송이버섯, 차가버섯, 표고버섯, 상황버섯, 잎새버섯, 노루궁뎅이버섯, 영지버섯들 중에서 선택된 2종이상의 종균(種菌)을 곡물배지에 접종하는 단계;
배양용기의 마개를 닫고 24∼30℃의 온도를 유지하는 배양실에서 9∼12일간 배양시키는 단계;
2종류 이상의 버섯이 돋아난 곡물배지를 멸균기에 투입하여 1∼2기압(氣壓) 및 120∼130℃의 온도를 부여하여 10∼20분간 멸균시키는 단계;
멸균이 완료된 버섯과 곡물배지를 45∼55℃의 온도를 부여하여 4∼6시간 건조시키는 단계;
건조가 완료된 버섯과 곡물배지를 함께 분쇄하여 100∼300메시의 입도(粒度)를 갖는 체로 걸러서 버섯분말을 완성하는 단계로 이루어진 것을 특징으로 하는 발효곡물 배지를 이용한 복합버섯분말의 제조방법.
Fermenting the grains at a temperature of 22 to 45 캜 for 2 to 5 hours by immersing the grains in a complex microorganism fermentation broth;
Dehydrating the fermented cereal to form a cereal medium in which the moisture content is maintained at 30 to 50%;
A step of putting the grain medium into a sterilized culture container and inoculating two or more kinds of seeds selected from pine mushroom, mushroom, shiitake mushroom, mushroom, mushroom, mushroom, and myrtle into a grain medium;
Incubating the culture vessel for 9 to 12 days in a culture room in which the temperature is maintained at 24 to 30 DEG C by closing the cap of the culture vessel;
Adding at least two types of mushroom-grown cereal medium to a sterilizer, sterilizing the mixture at a temperature of 1 to 2 atmospheres and a temperature of 120 to 130 DEG C for 10 to 20 minutes;
Drying the sterilized mushroom and the cereal medium at a temperature of 45 to 55 캜 for 4 to 6 hours;
And a step of grinding the dried mushroom and the cereal medium together and filtering the dried mushroom with a sieve having a particle size of 100 to 300 mesh to complete the mushroom powder.
상기 복합미생물 발효액에 침지(浸漬)되는 곡물은 콩, 팥, 수수, 기장, 차, 조, 율무, 현미, 보리, 흑미, 밀, 녹두 중에서 어느 하나이거나 이들의 조합으로 구성된 군에서 선택된 것이고,
상기 곡물의 발효에 사용되는 복합미생물 발효액은,
생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 가하고 유산균을 부여하여 발효시킨 다음 가압 증류하여 획득한 추출물을 숙성 발효시키는 1 차 유산균 발효단계;
1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 발효시키는 2차 유산균 발효단계, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 발효시키는 3차 효모균 발효단계;
3차 효모균 발효물에 방선균·사상균·고초균·황국균·초산균을 접종하고 발효시키는 4차 발효단계;
4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 발효시키는 5차 발효단계에 의하여 복합미생물 발효액이 완성된 것을 특징으로 하는 발효곡물 배지를 이용한 복합버섯분말의 제조방법.
The method according to claim 1,
The grains to be immersed in the complex microorganism fermentation broth are selected from the group consisting of soybean, red bean, sorghum, millet, tea, sake, yulmu, brown rice, barley, black rice, wheat, mung bean,
The complex microorganism fermentation broth used for the fermentation of the cereals comprises,
Fermenting the primary lactobacillus fermenting the fermented product by adding 0.3 to 0.8 wt% of salt to the total weight of the herbal medicines, adding lactic acid bacteria to the fermented product, and then subjecting the obtained extract to fermentation;
A second lactic acid fermentation step of adding 0.1 to 0.5% by weight of water-soluble propolis to the first lactic acid fermented product and fermenting the same by adding lactic acid bacteria; adding 0.5 to 2% by weight of minerals to the second lactic acid fermented product; Fermenting the third yeast strain to ferment;
A fourth fermentation step of inoculating and fermenting actinomycetes, filamentous fungi, Bacillus subtilis, Hwang gukyun, and acetic acid bacteria in the fermented product of the third yeast;
Wherein the mixed microbial fermentation broth is completed by adding 5 to 10% by weight of glycerin to the 4 < th > fermentation product and then homogenizing at high speed and then fermenting the fermented product. Way.
고르게 섞여진 가루분말을 물과 혼합하여 두유(豆乳)를 만들고, 가열용기에 두유를 넣고 110∼120℃의 온도로 가열하여 증숙하는 단계;
가열된 두유를 식힌 후 천연재료 응고제를 투입하고 고르게 저어 응고시키는 단계;
응고된 재료를 포장용기에 투입하고 밀봉하여 두부의 형태를 유지한 상태에서 완전히 응고되어 전두부가 완성되는 단계로 구성된 것으로 특징으로 하는 복합버섯분말을 이용한 전두부의 제조방법.
27 to 31 wt% of the composite mushroom powder obtained by the manufacturing method of claim 1, 66 to 70 wt% of soybean flour peeled and ground with soybean, and 2 to 4 wt% of vegetable powder;
Mixing soymilk powder with water to make soymilk, adding soymilk to a heating vessel, and heating the mixture at a temperature of 110 to 120 캜;
Cooling the heated soymilk, adding a natural material coagulant, and stirring and evenly mixing the natural coagulant;
Wherein the solidified material is put into a packaging container and sealed to complete the frontal portion while maintaining the shape of the tofu, thereby completing the frontal portion.
상기 야채가루는 백년초, 당근, 치자, 시금치, 클로렐라(chlorella) 중 어느 하나이거나 이들의 조합으로 구성된 군에서 선택된 것을 건조시킨 후 200∼300메시의 입도(粒度)를 갖도록 분쇄한 것이고,
상기 천연재료 응고제는 구기자, 복분자, 오미자, 함초, 신선초 중 어느 하나이거나 이들의 조합으로 구성된 군에서 선택하였으며, 천연재료를 분쇄하고, 유산균으로 6∼8일 발효시킨 후 두유에 대해 0.8∼2중량% 첨가하여 사용한 것을 특징으로 하는 복합버섯분말을 이용한 전두부의 제조방법.The method of claim 3,
The vegetable powder may be one selected from the group consisting of 100 milliseconds, carrot, gardenia, spinach, chlorella, or a combination thereof, and then pulverized to have a particle size of 200 to 300 mesh.
Wherein the natural material coagulant is selected from the group consisting of gugija, bokbunja, omija, green tea, and kuzuchu, or a combination thereof. The natural material is pulverized and fermented with lactic acid bacteria for 6-8 days, % Of the total mushroom powder.
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CN105029375A (en) * | 2014-09-20 | 2015-11-11 | 林静 | Preparation method of sour-and-hot flammulina velutipes |
KR101589250B1 (en) * | 2014-08-13 | 2016-01-27 | 박순철 | Hericium erinaceum Containing Bean Curd And Method for Producing the Same |
KR101656861B1 (en) * | 2016-04-01 | 2016-09-23 | 주식회사 아미코스메틱 | Methods of producing Ganoderma lucidum mycelia having enhanced ganodermanondiol content |
CN111867402A (en) * | 2019-01-30 | 2020-10-30 | 吉运康股份有限公司 | Agaricus campestris composite mycelium composition with liver function improving activity and its preparation method |
KR102402408B1 (en) * | 2021-05-14 | 2022-05-30 | 허서진 | Complex culture method for agaricus mushroom and multi-species mushroom mycelium |
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KR101589250B1 (en) * | 2014-08-13 | 2016-01-27 | 박순철 | Hericium erinaceum Containing Bean Curd And Method for Producing the Same |
CN105029375A (en) * | 2014-09-20 | 2015-11-11 | 林静 | Preparation method of sour-and-hot flammulina velutipes |
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KR102402408B1 (en) * | 2021-05-14 | 2022-05-30 | 허서진 | Complex culture method for agaricus mushroom and multi-species mushroom mycelium |
CN114940948A (en) * | 2022-04-12 | 2022-08-26 | 天津尚美化妆品有限公司 | Tricholoma matsutake and tricholoma matsutake mycelium fermentation medium and preparation method of fermentation liquid |
CN114940948B (en) * | 2022-04-12 | 2023-10-20 | 天津尚美化妆品有限公司 | Tricholoma matsutake and tricholoma matsutake mycelium fermentation medium and preparation method of fermentation broth |
CN116035175A (en) * | 2022-10-21 | 2023-05-02 | 中国人民解放军陆军勤务学院 | Novel plateau animal and plant composite functional rod and preparation method thereof |
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