CN110812429A

CN110812429A - Pharmaceutical composition and syrup

Info

Publication number: CN110812429A
Application number: CN201911309187.2A
Authority: CN
Inventors: 张峰; 李晓明
Original assignee: Zhuohe Pharmaceutical Group Co Ltd
Current assignee: Zhuohe Pharmaceutical Group Co Ltd
Priority date: 2019-12-18
Filing date: 2019-12-18
Publication date: 2020-02-21

Abstract

The invention discloses a pharmaceutical composition and a syrup. The pharmaceutical composition is prepared by the following steps: a first mixture, wherein the first mixture is prepared from tree peony bark, rehmannia root, dogwood, Chinese yam, tuckahoe, alisma orientale, astragalus and codonopsis pilosula; a second mixture, wherein the second mixture is prepared from lygodium japonicum stem, sargentgloryvine stem, dandelion, giant knotweed rhizome and motherwort herb; and the second mixture is mixed with the first mixture. The syrup has good therapeutic effect on frequent micturition, urgent micturition, and odynuria.

Description

Pharmaceutical composition and syrup

FIELD

The invention relates to a traditional Chinese medicine, in particular to a pharmaceutical composition and syrup.

Background

The downward flow of damp-heat is also called damp-heat in the lower-jiao, which is the term of the disease in traditional Chinese medicine, and refers to the condition of the mixture of damp-heat and heat invading the lower-jiao, manifested as scanty and brownish urine, fatigue, yellow and greasy tongue coating, poor appetite, etc. Clinically, it is commonly seen in diarrhea due to damp-heat, stranguria with turbid urine, retention of urine, pruritus vulvae and leucorrhea, and the bladder is most easily invaded and common, so the downward flow of damp-heat refers to damp-heat in the bladder. Downward flow of damp-heat is mainly manifested as frequent, urgent and painful urination, and modern medical research should be in western medicine for urinary tract infection, cystitis, prostatitis, pyelonephritis, etc.

Downward flow of damp-heat is usually caused by invasion of the bladder by exogenous pathogenic damp-heat; or the food is careless, and the pungent and pungent food is liked to be eaten, so that damp-heat is generated and flows downwards to the bladder. Generally speaking, downward flow of damp-heat is caused by the dysfunction of qi movement in the bladder due to damp-heat pathogen. This is the understanding of the etiology and pathogenesis of downward flow of damp-heat in traditional Chinese medicine.

In the aspect of specific symptoms, the traditional Chinese medicine thinks that the damp-heat stagnates and evaporates the bladder, the qi transformation is not smooth, and the urethra is pressed, so the frequent micturition, urgent micturition, painful urination and the like appear. Damp-heat is the pathogenic heat, and heat tends to damage the body fluids, so the body fluids are burned, resulting in short, yellow urine. Damp-heat damaging the blood vessels promotes recklessly blood flow, resulting in hematuria. If the damp-heat lasts for a long time, the urine becomes turbid and becomes sand. Damp-heat usually invades the bladder, but it reaches the lower abdomen and waist, causing pain in the lower abdomen and waist due to the adverse flow of qi. The tongue pulse with damp-heat is mostly red, yellow and greasy coating and slippery and rapid pulse.

Aiming at the symptoms of frequent micturition, urgent micturition, odynuria and the like caused by downward flow of damp-heat in traditional Chinese medicine, the western medicine is the most commonly used antibiotics for symptomatic treatment at present, but the western medicine has great inherent side effect and is not ideal in treatment effect. At present, the traditional Chinese medicine treatment of downward flow of damp-heat generally takes heat clearing and diuresis as main components, the traditional Chinese medicine can select medicines for promoting urination, treating stranguria and diminishing inflammation, has definite treatment effect and small side effect, and can play a role in treating both symptoms and root causes, but the traditional Chinese medicine formula needs dialectical treatment, has reasonable formula and also needs to select genuine medicinal materials.

SUMMARY

In one aspect, the disclosure relates to a pharmaceutical composition prepared by the steps of:

a first mixture, wherein the first mixture is prepared from tree peony bark, rehmannia root, dogwood, Chinese yam, tuckahoe, alisma orientale, astragalus and codonopsis pilosula; and

a second mixture, wherein the second mixture is prepared from lygodium japonicum stem, sargentgloryvine stem, dandelion, giant knotweed rhizome and motherwort herb; and

the second mixture is mixed with the first mixture

In another aspect, the present disclosure relates to a syrup made by the steps of: mixing the above medicinal composition with adjuvants.

Detailed description of the invention

In the following description, certain specific details are included to provide a thorough understanding of various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments can be practiced without one or more of the specific details, or with other methods, components, materials, and so forth.

Unless otherwise required by the disclosure, throughout the specification and the appended claims, the words "comprise", "comprising", and "have" are to be construed in an open, inclusive sense, i.e., "including but not limited to".

Reference throughout the specification to "one embodiment," "an embodiment," "in another embodiment," or "in certain embodiments" means that a particular reference element, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" or "in another embodiment" or "in certain embodiments" in various places throughout this specification are not necessarily all referring to the same embodiment, and furthermore, particular elements, structures, or features may be combined in any suitable manner in one or more embodiments.

Definition of

In the present disclosure, moutan bark is the dried root bark of Paeonia suffruticosa Andr. Cortex moutan has the following properties: bitter, pungent and slightly cold. The tree peony bark has the functions: clear heat and cool blood, activate blood and resolve stasis. The moutan bark is used for treating: can be used for treating heat syndrome of nutrient-blood, macula due to toxic heat, hematemesis and epistaxis, nocturnal fever, cold morning, anhidrosis, amenorrhea, dysmenorrhea, traumatic injury, carbuncle, swelling, and sore.

In the present disclosure, rehmannia is a fresh or dried root tuber of rehmannia glutinosa Libosch, a plant of Scrophulariaceae. The rehmanniae radix includes fresh rehmanniae radix and radix rehmanniae.

Wherein, the nature and taste of fresh rehmannia: sweet, bitter and cold; dried rehmannia root, radix rehmanniae, property and taste: sweet and cold in nature.

Fresh rehmannia functions: clearing heat, promoting fluid production, cooling blood, and stopping bleeding; the function of the dried rehmannia root: clearing away heat and cooling blood, nourishing yin and promoting the production of body fluid.

The fresh rehmannia root is mainly used for treating: can be used for treating fever with yin impairment, crimson and polydipsia of tongue, toxic plaque, hematemesis, epistaxis, and swollen and painful throat; the dried rehmannia root is mainly used for treating: can be used for treating yin deficiency, fever, internal heat, diabetes, yin deficiency, bone steaming, internal heat, and hemorrhage.

In the present disclosure, the dogwood is dried ripe pulp of mountain lei of mountain leicaceae. The characteristic taste of dogwood: sour, astringent and slightly warm. Function of dogwood: tonify liver and kidney, astringe and induce depletion. The dogwood is used for treating: can be used for treating vertigo, tinnitus, soreness of waist and knees, sexual impotence, spermatorrhea, enuresis, frequent micturition, metrorrhagia, leukorrhagia, sweating, asthenia, internal heat, and diabetes.

In the present disclosure, yam is a dried rhizome of dioscorea opposita of the family dioscoreaceae. The nature and taste of the Chinese yam: sweet and neutral. The functions of the Chinese yam are as follows: tonify spleen and stomach, promote the production of body fluid and nourish lung, tonify kidney and astringe essence. The Chinese yam is mainly used for treating: can be used for treating spleen deficiency, anorexia, chronic diarrhea, lung deficiency, cough, asthma, spermatorrhea, leukorrhagia, frequent micturition, and diabetes due to deficiency heat. The bran-parched rhizoma Dioscoreae has effects of invigorating spleen and invigorating stomach. Can be used for treating spleen deficiency, anorexia, diarrhea, loose stool, and leukorrhagia.

In the present disclosure, poria is the dried sclerotium of poria cocos, a fungus of the family Polyporaceae. Poria cocos is of a nature and taste: sweet, bland and mild. The function of the tuckahoe is as follows: induce diuresis and drain dampness, invigorate spleen and calm heart. The tuckahoe mainly treats the following diseases: can be used for treating edema, oliguria, phlegm and fluid retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

In the present disclosure, alisma orientale is a dried tuber of alisma orientale of the family alismatis. The nature and taste of the rhizoma alismatis: sweet, bland and cold. The function of the alisma orientale is as follows: induce diuresis and drain dampness, purge heat, resolve turbidity and reduce blood fat. The main treatment of the rhizoma alismatis is as follows: can be used for treating dysuria, edema, abdominal distention, diarrhea, oliguria, phlegm retention, vertigo, pyretic stranguria, pain, and hyperlipidemia.

In the present disclosure, the astragalus root product is the dried root of astragalus mongholicus or astragalus membranaceus of leguminosae. The nature and taste of astragalus: sweet and warm. The function of the astragalus root is as follows: invigorating qi, invigorating yang, consolidating superficial resistance, arresting sweating, inducing diuresis, relieving swelling, promoting fluid production, nourishing blood, removing stagnation, relieving arthralgia, expelling toxin, expelling pus, healing sore, and promoting granulation. The astragalus root is mainly used for treating: can be used for treating deficiency of vital energy, asthenia, anorexia, loose stool, collapse of middle-warmer energy, chronic diarrhea, rectocele, hematochezia, spontaneous perspiration due to exterior deficiency, edema due to qi deficiency, internal heat, diabetes, blood deficiency, hemiplegia, arthralgia, numbness, carbuncle, cellulitis, and intractable ulcer.

In the present disclosure, the codonopsis pilosula is a dried root of codonopsis pilosula, codonopsis pilosula vegetarian or codonopsis pilosula from the family of sterculia. The nature and taste of the codonopsis pilosula: sweet and neutral. The function of the codonopsis pilosula is as follows: to invigorate the spleen, benefit the lung, nourish blood and promote the production of body fluid. The codonopsis pilosula is mainly used for treating: can be used for treating deficiency of spleen-lung qi, anorexia, listlessness, cough, asthma, deficiency of qi and blood, sallow complexion, palpitation, short breath, thirst due to body fluid consumption, and internal heat.

In the present disclosure, the climbing fern is a mature spore of climbing fern spore of the perennial climbing fern of the family lygodiaceae. The nature and taste of the honeysuckle sargentgloryvine stem: sweet and cold in nature. The function of the golden sargentgloryvine stem is as follows: clear heat and remove toxicity, induce diuresis and treat stranguria. The main treatment of the honeysuckle sargentgloryvine stem is as follows: it can be used for treating urinary tract infection, lithangiuria, leukorrhagia, dysuresia, nephritis edema, jaundice due to damp-heat pathogen, common cold, fever, cough, sore throat, enteritis, dysentery, scald, and erysipelas.

In the present disclosure, sargentgloryvine stem is a dried rattan of sargentgloryvine stem, a plant of the family akebiaceae. Caulis Sargentodoxae in nature: bitter and neutral. The sargentgloryvine stem has the functions: clearing away heat and toxic materials, promoting blood circulation, dispelling pathogenic wind, and relieving pain. The sargentgloryvine stem is mainly used for treating: can be used for treating abdominal pain due to intestinal abscess, pyocutaneous disease due to heat toxin, amenorrhea, dysmenorrhea, traumatic injury, swelling and pain, and rheumatalgia.

In the present disclosure, the dandelion is a dried whole plant of dandelion of the family of Compositae, dandelion from the genus of Aralia or several plants of the same genus. The dandelion is characterized by flavor: bitter, sweet and cold. The dandelion has the following functions: clearing away heat and toxic material, dispersing swelling and dissipating stagnation, inducing diuresis and treating stranguria. The dandelion is mainly used for treating: can be used for treating furuncle, swelling, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, pulmonary abscess, intestinal abscess, jaundice due to damp-heat pathogen, and stranguria with astringency and pain due to heat.

In the present disclosure, polygonum cuspidatum is a dried rhizome and root of polygonum cuspidatum, a family polygonaceae. Polygonum cuspidatum has the following properties: slightly bitter and slightly cold. Function of giant knotweed rhizome: promoting diuresis, removing jaundice, clearing heat, removing toxic substances, removing blood stasis, relieving pain, relieving cough, and eliminating phlegm. The main treatment of the giant knotweed rhizome is as follows: can be used for treating jaundice due to damp-heat pathogen, stranguria with turbid urine, leukorrhagia, rheumatalgia, carbuncle, swelling, sore, scald due to hot water and fire, amenorrhea, abdominal mass, traumatic injury, and cough due to lung heat.

In the present disclosure, leonurus is fresh or dried aerial parts of leonurus japonicus belonging to the family labiatae. Motherwort herb with characteristic taste: bitter, pungent and slightly cold. The motherwort functions: promoting blood circulation to regulate menstruation, inducing diuresis to alleviate edema, clearing away heat and toxic materials. The motherwort is mainly used for treating: can be used for treating menoxenia, dysmenorrhea, amenorrhea, lochiorrhea, edema, oliguria, pyocutaneous disease, and toxic swelling.

In the present disclosure, the purified water is water for medical use, which is obtained by distilling drinking water, ion exchange method, reverse osmosis method or other suitable method, and does not contain any additive.

In the present disclosure, D70 refers to the corresponding particle size value when the cumulative distribution percentage from small to large reaches 70% in the particle size distribution. That is, the number of particles having a particle size of less than D70 in a powder is 70% of the total number of particles.

In the present disclosure, D90 refers to the corresponding particle size value when the cumulative distribution percentage from small to large reaches 90% in the particle size distribution. That is, the number of particles having a particle size of less than D90 in a powder is 90% of the total number of particles.

In the present disclosure, supercritical extraction is extraction using a gas in a state exceeding a critical temperature and a critical pressure as a solvent.

In the present disclosure, mesh refers to the unit of measure, and the unit of measure mesh size refers to the size of the raw material particles, generally expressed as the maximum length of the particles. The mesh is the size representing the mesh size of a standard screen. In the taylor standard sieve, the so-called mesh is the number of sieve openings in a 1 inch length and is simply called mesh.

Detailed Description

the second mixture is mixed with the first mixture.

Wherein, the golden sargentgloryvine stem, the sargentgloryvine stem and the dandelion are monarch drugs and have main treatment effects on frequent micturition, urgent micturition and odynuria; cortex moutan, rhizoma Polygoni Cuspidati, rehmanniae radix, and Corni fructus are ministerial drugs to assist principal drugs in therapeutic effect; the yam, the tuckahoe, the rhizoma alismatis and the motherwort are adjuvant drugs and used for enhancing the treatment effect of monarch drugs and ministerial drugs; radix astragali and radix codonopsitis are guiding drugs, and have the functions of supporting the body resistance and leading the meridian into the diseased root.

In certain embodiments, the first component is made from 40 to 60 parts of moutan bark, 40 to 60 parts of rehmannia root, 40 to 60 parts of cornus officinalis, 5 to 10 parts of Chinese yam, 5 to 10 parts of poria cocos, 5 to 10 parts of alisma orientale, 30 to 50 parts of astragalus root and 30 to 50 parts of codonopsis pilosula by weight.

In certain embodiments, the second component is made from, by weight, 60 to 80 parts of cissampelos mutica, 60 to 80 parts of sargentgloryvine stem, 60 to 80 parts of dandelion, 40 to 60 parts of polygonum cuspidatum, and 5 to 10 parts of motherwort herb.

In certain embodiments, the particle size D70 of the first mixture is 3.5 μm or less.

In certain embodiments, the particle size D90 of the first mixture is 3.5 μm or less.

In certain embodiments, the first mixture is made by pulverizing.

In certain embodiments, the first mixture is also subjected to a second pulverization.

In certain embodiments, the secondary comminution is low temperature fluidized comminution. Adopts low-temperature pulverization technology, namely a low-temperature fluidization pulverizer is used for pulverizing the medicinal materials into superfine powder or superfine powder to achieve the purpose of breaking cell walls and enhancing the medicine absorption.

In certain embodiments, the temperature of the low temperature fluidized comminution is from-50 ℃ to 0 ℃.

In certain embodiments, the temperature of the low temperature fluidized comminution is from-30 ℃ to-35 ℃.

In certain embodiments, the temperature of the low temperature fluidized comminution is-30 ℃.

In some embodiments, the second component is obtained by extracting cissampelos mutica, sargentgloryvine stem, dandelion, giant knotweed rhizome and motherwort herb.

In some embodiments, the second component is obtained by subjecting lygodium japonicum stem, sargentgloryvine stem, dandelion, giant knotweed rhizome and motherwort to supercritical extraction. By adopting the supercritical extraction technology, the effective components in the medicinal materials are extracted to the maximum extent, and the damage of high-temperature extraction on the effective components is avoided.

In certain embodiments, the extraction is performed using a non-polar extractant or a polar extractant.

In certain embodiments, the non-polar extractant is selected from carbon dioxide, alkane-based extractants, or mixtures thereof.

In certain embodiments, the polar extractant is selected from an alcohol extractant, a ketone extractant, or a mixture thereof.

In certain embodiments, the alkane-based extractant is selected from ethane, propane, butane, cyclohexane, or mixtures thereof.

In certain embodiments, the alcohol extractant is selected from methanol, ethanol, or mixtures thereof.

In certain embodiments, the ketone extractant is selected from the group consisting of isopropyl ketone, acetone, or mixtures thereof.

In certain embodiments, the extraction is preceded by comminution, which has a particle size of 20 to 100 mesh.

In certain embodiments, the extraction is preceded by comminution, which has a particle size of 20 to 50 mesh.

In certain embodiments, the extraction is preceded by comminution, which has a particle size of 40 mesh.

In another aspect, the present disclosure relates to a syrup made by the steps of: mixing the medicinal composition with auxiliary materials, wherein the medicinal composition is prepared by the following steps:

the second mixture is mixed with the first mixture.

In certain embodiments, the adjunct is selected from the group consisting of dry syrup, menthol, honey, maltose or mixtures thereof.

Detailed Description

The present invention is described in detail below with reference to various embodiments, but it should be understood that these embodiments are not intended to limit the present invention, and those skilled in the art should be able to make modifications and substitutions on the functions, methods, or structures of these embodiments without departing from the scope of the present invention.

Studies show that the honeysuckle sargentgloryvine stem, the sargentgloryvine stem and the dandelion are sweet in nature and cold in nature. The traditional Chinese medicine composition belongs to the urinary bladder and small intestine channels, has good curative effects on urinary tract infection, lithangiuria, leucorrhea with turbid urine, dysuresia, nephritic edema, damp-heat jaundice, cold and fever, cough, sore throat, enteritis, dysentery, scald and erysipelas, and is particularly suitable for frequent micturition, urgent micturition and odynuria caused by downward flow of damp-heat; cortex moutan, rhizoma Polygoni Cuspidati, rehmanniae radix, and Corni fructus are cold in nature, bitter in taste and cool in flavor, and enter heart, liver, kidney, and lung channels, and can be used for adjuvant treatment of pollakisuria, urgent micturition, odynuria, rhizoma Dioscoreae, Poria, Alismatis rhizoma, and herba Leonuri with sweet and light properties, and enter heart, lung, spleen, and kidney channels, and can enhance curative effects of caulis Kadsurae Longipedunculatae, caulis Sargentodoxae, and herba Taraxaci, and radix astragali and radix Codonopsis can be added to enhance qi and blood, and introduce the medicine into channels.

The invention adopts advanced low-temperature crushing and extraction technology, can increase drug absorption and enhance curative effect.

Example 1

The embodiment discloses a pharmaceutical composition for treating frequent micturition, urgent micturition and odynuria caused by downward flow of damp-heat, which is in the form of syrup and consists of the following components:

composition (I)	Dosage per gram
		Caulis et folium Hedyotis Hedyotideae	600
Caulis Sargentodoxae	600
		Dandelion	600
Cortex moutan	400
		Giant knotweed rhizome	400
Rehmannia root	400
		Fructus Corni	400
Chinese yam	50
		Poria cocos (Schw.) wolf	50
Rhizoma alismatis	50
		Motherwort herb	50
Radix astragali	300
		Codonopsis pilosula	300
Dry syrup	200
		Menthol crystal	0.6
Honey	60
		Maltose	20

The preparation process comprises the following steps:

1. putting cortex moutan, rehmanniae radix, Corni fructus, rhizoma Dioscoreae, Poria, Alismatis rhizoma, radix astragali, and radix Codonopsis into universal pulverizer, primarily pulverizing, and pulverizing into coarse powder;

2. starting the ultralow temperature fluidization pulverizer, cooling to-30 ℃, starting the gas cold dryer to pre-cool high-pressure gas, enabling the coarse powder to enter a pulverizing cavity along with the high-pressure gas through a Venturi tube, adjusting the gas pressure, starting pulverizing, separating materials through a cyclone separator, and collecting the ultrafine powder with the particle size D90 being less than or equal to 3.5 mu m from a collecting barrel, and sealing for later use;

3. pulverizing 5 Chinese medicinal materials including caulis Sargentodoxae, herba Taraxaci, rhizoma Polygoni Cuspidati, and herba Leonuri with universal pulverizer, sieving with 40 mesh sieve, and removing impurities;

4. then placing the dried coarse powder into a supercritical extraction tower, starting a refrigeration switch, simultaneously liquefying an extractant through a condensing tube, and then liquefying liquid CO₂Pumping into an extraction tower, increasing pressure of the extraction tower to 15MPa, extracting, and extracting to obtain extracted CO containing effective components₂The extractant is transferred to a flow splitting device for separation, and liquid CO is separated₂Converting the extractant into gas state to separate effective components, and sealing and storing the separated components for later use;

5. adding the superfine powder obtained in the step 2 into dry syrup, menthol, honey and maltose, and uniformly stirring to obtain thick syrup;

6. adding the effective components extracted in the step 4 into the solution, stirring uniformly, and subpackaging into bottles to obtain the traditional Chinese medicine composition.

Example 2

the preparation process comprises the following steps:

Example 3

results of pharmacodynamics experiments

1. Laboratory animals and groups

Experimental animals: male rabbits with weight of 1.5-2.0kg and 24 rabbits were randomly divided into blank group (n-8), model group (n-8) and treatment group (n-8), and the strain was Escherichia coli, and was prepared into 10 when used^7-9A bacterial liquid of/ml;

2. making of models

The blank group is not molded, the treatment group and the model group are molded, the animal is normally induced to urinate before molding, the rabbit is fixed in a standing position during molding, the urine in the bladder is emptied, then diluted hydrochloric acid with the pH value of 3 is injected into the rabbit through a catheter for 4 times and is kept for 10 minutes when being injected for the first time and the second time, then the liquid in the bladder is emptied, and then 10 is injected into the rabbit^7-9The/ml bacteria solution is opened after closing the urinary tract for 4 hours, the catheter is taken out, and the animals are put back into the rearing cage to eat and drink freely. The animals were then observed for condition.

On the day of animal pre-modeling, the treatment groups of example 1 were ground and added with water to prepare an aqueous solution for administration, and the blank group and the model group were given with physiological saline.

3. Observation index

Urination condition: after the model making administration, the times of 6h urination and the single urination amount of each group of animals are respectively counted, and the specific statistics is shown in the following table.

TABLE 16 h number of urination

Number of days	Blank group	Model set	Treatment group
				Day 1	1.12±0.63	2.05±0.96	1.72±0.57
Day 2	1.06±0.26	1.95±0.87	1.73±0.69
				Day 3	0.95±0.59	3.15±0.69	2.12±0.49
Day 4	1.65±0.65	2.65±0.69	1.63±0.69
				Day 5	2.03±0.39	3.16±0.73	1.49±0.57
Day 6	1.75±0.35	2.69±0.93	1.62±0.59
				Day 7	1.00±0.69	2.68±0.72	1.56±0.49
Day 8	1.05±0.89	3.26±0.65	1.13±0.68
				Day 9	1.35±0.68	2.69±0.89	1.29±0.41
Day 10	1.06±0.78	3.67±0.86	1.24±0.61

Note: comparing the model group with the blank group, wherein P is less than 0.05;

the treatment group compared to the model group had P < 0.05.

TABLE 1 Single urine output statistics (unit: ml)

Number of days	Blank group	Model set	Treatment group
				Day 1	11.2±3.6	16.1±4.2	15.3±4.9
Day 2	10.4±6.2	14.4±6.9	12.8±5.1
				Day 3	9.5±3.9	16.9±3.7	13.5±5.6
Day 4	8.5±6.7	13.6±6.8	12.4±5.6
				Day 5	13.6±3.7	15.6±3.9	12.3±6.1
Day 6	15.6±5.6	17.6±6.4	15.9±6.8
				Day 7	15.7±6.8	17.9±6.1	16.2±4.9
Day 8	13.9±5.7	16.2±5.1	12.7±3.8
				Day 9	16.1±6.8	19.1±6.3	15.8±6.2
Day 10	13.9±5.9	15.3±7.3	13.1±5.5

the treatment group compared to the model group had P < 0.05.

The above experiments show that the symptoms of animals are reduced after the treatment with the preparation of example 1 of the present invention, which indicates that the granules of the example have good therapeutic effects on frequent micturition, urgent micturition and odynuria.

From the foregoing it will be appreciated that, although specific embodiments of the disclosure have been described herein for purposes of illustration, various modifications or improvements may be made by those skilled in the art without departing from the spirit and scope of the disclosure, and that such modifications or improvements are intended to be within the scope of the appended claims.

Claims

1. A pharmaceutical composition prepared by the steps of:

a first mixture, wherein the first mixture is prepared from tree peony bark, rehmannia root, dogwood, Chinese yam, tuckahoe, alisma orientale, astragalus and codonopsis pilosula;

the second mixture is mixed with the first mixture.

2. The pharmaceutical composition of claim 1, wherein the first mixture is made from, by weight, 40 to 60 parts of moutan bark, 40 to 60 parts of rehmannia, 40 to 60 parts of cornus officinalis, 5 to 10 parts of Chinese yam, 5 to 10 parts of poria cocos, 5 to 10 parts of alisma orientale, 30 to 50 parts of astragalus membranaceus and 30 to 50 parts of codonopsis pilosula.

3. The pharmaceutical composition of claim 1 or 2, wherein the second mixture is made from, by weight, 60 to 80 parts of cissampelos mutica, 60 to 80 parts of sargentgloryvine stem, 60 to 80 parts of dandelion, 40 to 60 parts of polygonum cuspidatum, and 5 to 10 parts of motherwort.

4. The pharmaceutical composition according to any one of claims 1 to 3, wherein the particle size D70 ≤ 3.5 μm, preferably D90 ≤ 3.5 μm.

5. The pharmaceutical composition according to any one of claims 1 to 4, wherein the first mixture is prepared by pulverization, preferably the first mixture is further subjected to a second pulverization, preferably the second pulverization is a low temperature fluidized pulverization, more preferably the temperature of the low temperature fluidized pulverization is from-50 ℃ to 0 ℃, preferably from-30 ℃ to-35 ℃, more preferably from-30 ℃.

6. The pharmaceutical composition according to any one of claims 1 to 5, wherein the second mixture is obtained by extraction of Cissus quadrangularis, Sargentodoxa cuneata, Taraxacum officinale, Polygonum cuspidatum, and Leonurus sibiricus, preferably by supercritical extraction, preferably by using a non-polar extractant or a polar extractant, preferably selected from carbon dioxide, alkane-based extractants, or mixtures thereof; preferably, the polar extractant is selected from an alcohol extractant, a ketone extractant, or a mixture thereof; more preferably, the alkane extractant is selected from ethane, propane, butane, cyclohexane or mixtures thereof; more preferably the alcohol extractant is selected from methanol, ethanol or mixtures thereof; more preferably, the ketone extractant is selected from the group consisting of isopropyl ketone, acetone, or mixtures thereof.

7. A pharmaceutical composition according to claim 6, wherein the particle size after comminution, preferably comminution before extraction, is 20 to 100 mesh, more preferably 20 to 50 mesh, more preferably 40 mesh.

8. A syrup prepared by the steps of:

mixing a pharmaceutical composition according to any one of claims 1 to 7 with an excipient.

9. The syrup of claim 8 wherein the excipient is selected from dry syrup, menthol, honey, maltose or mixtures thereof.