CN110810438A - Phytophthora nicotianae nano copper oxide antibacterial agent and preparation method thereof - Google Patents
Phytophthora nicotianae nano copper oxide antibacterial agent and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A01N59/20—Copper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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Abstract
The invention relates to the technical field of antibacterial agent control, in particular to a phytophthora nicotianae nano copper oxide antibacterial agent and a preparation method thereof, wherein the main components of the nano copper oxide antibacterial agent are nano copper oxide and metalaxyl manganese zinc, and the mass ratio of the nano copper oxide to the metalaxyl manganese zinc is (25-200): 1. The invention aims to provide a phytophthora nicotianae nano copper oxide antibacterial agent and a preparation method thereof, which kill phytophthora nicotianae by utilizing the synergistic enhancement of nano copper oxide and metalaxyl manganese zinc, improve the bactericidal activity of the phytophthora nicotianae antibacterial agent, prevent or weaken the endocytosis between tobacco leaf cells and nano copper oxide, and prevent the growth of tobacco leaves from being blocked or weaken the influence on the quality of the tobacco leaves.
Description
Technical Field
The invention belongs to the technical field of antibacterial agent control, and particularly relates to a phytophthora nicotianae nano copper oxide antibacterial agent and a preparation method thereof.
Background
Tobacco is one of important economic crops in China and is an important economic support industry in China, however, as the continuous cropping age increases, the soil ecological environment becomes worse and the occurrence types of tobacco pests increase. Among them, a series of soil-borne diseases caused by phytophthora nicotianae, such as black shank, are prominent problems in tobacco plant protection. Once the disease caused by the pathogen is outbreak and epidemic, a large area of tobacco plants are usually killed, and huge economic loss is brought. At present, main technical measures aiming at the prevention and the treatment of the tobacco black shank comprise physical prevention and control, agricultural operation encouragement, disease-resistant variety breeding and chemical agent prevention and treatment, for example, treatment methods such as breeding disease-resistant varieties, high ridge cultivation, soil deep ploughing, crop rotation, biological antagonistic antibacterial agents and the like are adopted, but the operation modes have poor effects or cannot adapt to the current planting environment. At present, chemical control is still the main method for controlling phytophthora nicotianae in production.
With the rapid development of nanotechnology, nanomaterials are widely used in various fields such as energy, medicine, environmental protection, agriculture and the like. The nano copper oxide is widely applied to the field of pesticides due to the special bactericidal performance of the nano copper oxide, and is compounded with the pesticides to be used as the nano pesticides for killing bacteria. However, the particles formed during production or use may be unintentionally introduced into the soil and water, plants, and thus potentially harmful to the organism. The absorption of the plant to the nano copper oxide mainly crosses a cell membrane through endocytosis to enter a cell, and then enters a center pillar through an epidermis and a cortex, the toxic action to the plant is mainly shown in the influence on the growth of the plant, under the stress of the nano copper oxide, the plant grows slowly, seedlings are yellowed when the plant grows seriously, leaf edges are wilted, and the growth and the quality of tobacco leaves are greatly influenced, and the endocytosis of the plant is obstructed when the nano copper oxide is used as an antibacterial raw material, so that a nano antibacterial agent is urgently needed at present, the toxic action cannot be generated on the growth of the tobacco leaves, and the quality of the tobacco leaves cannot be influenced.
Disclosure of Invention
In view of the above, the present invention aims to provide a phytophthora nicotianae nano copper oxide antibacterial agent and a preparation method thereof, wherein the synergistic enhancement effect of the nano copper oxide and metalaxyl manganese zinc is used to kill phytophthora nicotianae, the bactericidal activity of the phytophthora nicotianae antibacterial agent is improved, the endocytosis between tobacco leaf cells and the nano copper oxide is prevented or weakened, and the influence on the tobacco leaf quality caused by the hindered growth of tobacco leaves is prevented or weakened.
In order to achieve the purpose, the invention provides the following technical scheme:
the phytophthora nicotianae nano copper oxide antibacterial agent mainly comprises nano copper oxide and metalaxyl manganese zinc, and the mass ratio of the nano copper oxide to the metalaxyl manganese zinc is (25-200): 1.
Furthermore, the particle size of the nano copper oxide is 20-100 nm.
Furthermore, in the phytophthora nicotianae nano copper oxide antibacterial agent, the concentration of the nano copper oxide is 25mg/L-100 mg/L.
Further, in the phytophthora nicotianae nano copper oxide antibacterial agent, the concentration of metalaxyl manganese zinc is 0.25mg/L-1 mg/L.
Further, in the phytophthora nicotianae nano copper oxide antibacterial agent, the concentration of nano copper oxide is 25mg/L, the concentration of metalaxyl manganese zinc is 0.5mg/L, and the mass ratio is 50: 1.
The invention also discloses a preparation method of the phytophthora nicotianae nano copper oxide antibacterial agent, which comprises the following steps:
(1) the method comprises the following steps of (1) preprocessing nano copper oxide, namely dispersing the nano copper oxide in 75-80 w% ethanol solution, introducing direct current, taking out after 20-30min, adding 25 wt% methyl- β -cyclodextrin solution, dispersing for 30-45min in an ultrasonic instrument with the frequency of 35kHz, heating in water bath until the water content is lower than 20 wt%, drying by using nitrogen, irradiating for 5min by using ultraviolet rays, and thus obtaining preprocessed nano copper oxide;
(2) preparing an antibacterial agent: adding the pretreated nano copper oxide into ultrapure water, adjusting the pH value to be 6-7, adding arabic gum, stirring, heating in a water bath to 35-45 ℃, stopping heating after the arabic gum is dissolved, naturally cooling, adding metalaxyl manganese zinc, and uniformly stirring to obtain the antibacterial agent.
Further, in the step (1), the direct current voltage is 10-12V, and the electrodes are all copper sheets.
Further, in the step (1), the weight ratio of the nano copper oxide to the methyl- β -cyclodextrin solution is 1: 1.
Further, in the step (2), the mass ratio of the Arabic gum to the nano-copper oxide is 0.01: 4.
The nano copper oxide is pretreated to cause the property of the nano copper oxide to be slightly changed, and the nano copper oxide is prevented from generating endocytosis under the conditions of not losing the effect of enhancing metalaxyl manganese zinc and killing tobacco phytophthora fungi, the charge density of the surface of the nano copper oxide is changed after the nano copper oxide is treated for 20-30min under a direct-current power supply, the activity is improved, the surface of the nano copper oxide is easily modified by methyl- β -cyclodextrin under the ultrasonic action, and the inhibition effect is presented, at the moment, the inhibition of the nano copper oxide generated by the plant cells on the nano copper oxide hinders or reduces the amount of the nano copper oxide entering the plant cells of the tobacco leaves, so the influence of the nano copper oxide on the growth of the tobacco leaves is reduced, and the accumulation amount of the nano copper oxide on the land and organisms is also reduced.
The pH value is adjusted to be weakly acidic or neutral, so that metalaxyl manganese zinc can be stabilized, the antibacterial agent can be stored for a longer time, the deposition of nano copper oxide can be prevented, and the influence on the growth of tobacco leaves is not easily caused under the weak acid or neutral condition. The particle size of the nano copper oxide is small, the surface energy and the specific surface area are large, agglomeration is easy to generate in the solution, after the Arabic gum is added, the agglomeration between the nano copper oxide particles can be prevented, the stability of the nano copper oxide particles in the solution is improved, and the nano copper oxide particles are dispersed more uniformly.
The invention has the beneficial effects that:
1. the invention provides a nano copper oxide antibacterial agent for phytophthora nicotianae, which kills phytophthora nicotianae by utilizing the synergistic enhancement effect of nano copper oxide and metalaxyl manganese zinc, improves the bactericidal activity of the antibacterial agent for phytophthora nicotianae, and provides a new control material and a new technical approach for controlling phytophthora nicotianae.
2. Through the pretreatment of the nano copper oxide, the endocytosis between tobacco leaf cells and the nano copper oxide is prevented or weakened, the influence on the tobacco leaf quality caused by the hindered growth of the tobacco leaves is prevented or weakened, the accumulation of the nano copper oxide in plants or soil is further prevented, and the accumulation of the nano copper oxide in organisms is further prevented.
3. When the concentration of the nano copper oxide in the phytophthora nicotianae nano copper oxide antibacterial agent is 25mg/L, the concentration of metalaxyl manganese zinc is 0.5mg/L, the mass ratio is 50:1, the effect is the best, and the synergistic factor reaches 1.51.
4. The phytophthora nicotianae nano copper oxide antibacterial agent is novel and safe, has little pollution, simple method and prominent effect, has higher synergistic interaction, can effectively overcome and delay the drug resistance of germs, reduces the using amount of pesticides and reduces the residue of the soil and crops of the pesticides.
Drawings
FIG. 1 is a graph showing the growth of Phytophthora nicotianae in an antibacterial property test of pretreated nano-copper oxide against Phytophthora nicotianae;
FIG. 2 is a scanning electron microscope observation image of the pretreated nano copper oxide on the inhibition of phytophthora nicotianae hyphae;
FIG. 3 is a diagram showing the germination of phytophthora nicotianae spores in an experiment for inhibiting the germination of phytophthora nicotianae spores by using the pretreated nano-copper oxide.
Detailed Description
Specific examples of the present invention will be described in detail below:
example 1: phytophthora nicotianae nano copper oxide antibacterial agent I
(1) And (2) pretreatment of the nano copper oxide, namely weighing 5g of the nano copper oxide with the particle size of 20-50nm, dispersing the nano copper oxide in 50ml of 75 w% ethanol solution, introducing direct current at 10V, taking out the nano copper oxide after electrifying for 20min, adding the nano copper oxide into 25 wt% methyl- β -cyclodextrin solution prepared from 5g of methyl- β -cyclodextrin, dispersing the nano copper oxide in an ultrasonic instrument with the frequency of 35kHz for 45min by ultrasonic wave, heating in water bath until the water content is lower than 20 wt%, drying by using nitrogen, and irradiating by using 340nm ultraviolet rays for 5min to obtain the pretreated nano copper oxide.
(2) Preparing an antibacterial agent: adding pretreated nano copper oxide into ultrapure water, adjusting the pH value to 6-6.5, adding 0.0125g of Arabic gum, stirring, heating in a water bath to 35 ℃, stopping heating after the Arabic gum is dissolved, naturally cooling, adding 0.2g of metalaxyl manganese zinc, and uniformly stirring to obtain the antibacterial agent, wherein the concentration of the nano copper oxide is 25mg/L and the concentration of the metalaxyl manganese zinc is 1 mg/L.
Example 2: phytophthora nicotianae nano copper oxide antibacterial agent II
(1) Pre-treating nano copper oxide, namely weighing 20g of nano copper oxide with the particle size of 50-100nm, dispersing the nano copper oxide in 120ml of 80 w% ethanol solution, introducing direct current at 12V, taking out the nano copper oxide after electrifying for 45min, adding 20g of methyl- β -cyclodextrin prepared 25 wt% of methyl- β -cyclodextrin solution, ultrasonically dispersing the nano copper oxide in an ultrasonic instrument with the frequency of 35kHz for 30min, heating in water bath until the water content is lower than 20 wt%, drying the nano copper oxide by using nitrogen, and irradiating the nano copper oxide by using 340nm ultraviolet rays for 5min to obtain the pre-treated nano copper oxide;
(2) preparing an antibacterial agent: adding the pretreated nano copper oxide into ultrapure water, adjusting the pH value to 6.5-7, adding 0.5g of Arabic gum, stirring, heating in a water bath to 45 ℃, stopping heating after the Arabic gum is dissolved, naturally cooling, adding 0.4g of metalaxyl manganese zinc, and uniformly stirring to obtain the antibacterial agent, wherein the concentration of the nano copper oxide is 20mg/L and the concentration of the metalaxyl manganese zinc is 0.4 mg/L.
Example 3 test of antibacterial property of pretreated Nano copper oxide against Phytophthora nicotianae
Six identical OA culture media subjected to high-temperature sterilization are prepared, the nano copper oxide subjected to the same pretreatment step in the step (1) in the example 1 is added into five OA culture media respectively, solid culture medium plates with the nano copper oxide concentration of 6.25, 12.5, 25, 50 and 100mg/L and the same total volume are prepared respectively, the rest one is used as a blank control group, and then 1 phytophthora cake with the diameter of 6mm is placed in the middle of each plate. The growth of colonies was observed.
The obtained results are shown in FIG. 1, and the colony growth is inhibited to different degrees compared with the control at 6.25, 12.5, 25 and 50mg/L, and the inhibition is strengthened along with the increase of the concentration; no phytophthora colonies appear on an OA solid medium plate containing 100mg/L of nano-copper oxide, which indicates that the pretreated nano-copper oxide has very good antibacterial property on phytophthora nicotianae.
Example 4: scanning electron microscope observation of pretreated nano copper oxide on phytophthora nicotianae hypha inhibition
Six identical media were prepared, numbered a1, a2, a3, b1, b2, b3, respectively. The phytophthora nicotianae cake after 3d culture was punched out several times with a punch (d ═ 6mm), and equal mass of the cake was inoculated into a medium containing 50mg/L of nano-copper oxide (b1, b2, b3) pretreated in the step (1) of example 1 and containing no nano-copper oxide (a1, a2, a3) and cultured for 3d (dark condition, 28 ℃). Then, an agar block containing fungal hyphae was cut from the edge of the medium, and the hyphae were collected and observed under microscopic examination using a scanning electron microscope.
The obtained results are shown in fig. 2, and the pretreated nano copper oxide (b1, b2, b3) has abnormal shapes of phytophthora hyphae, and the hyphae surface has concave-convex and collapse and other abnormal shapes, and is completely normal with the mycelium of the control group (a1, a2, a 3). The pretreated nano copper oxide has an inhibition effect on phytophthora nicotianae hyphae.
Example 5: experiment for inhibiting phytophthora nicotianae spore germination by using pretreated nano copper oxide
Activating phytophthora nicotianae, inducing to prepare spore suspension with concentration of 106one/mL. Preparing the nano copper oxide pretreated by the step (1) of the embodiment 1 into water solutions with different concentrations, and taking clean and sterile concave glassAnd taking six slides, sucking 10 mu L of nano copper oxide aqueous solution by using a gun head, respectively placing the nano copper oxide aqueous solution on five concave glass slides, wherein the concentrations of the nano copper oxide are respectively 100, 50, 25, 12.5 and 6.25mg/L, taking the rest blank control group, sucking 10 mu L of prepared spore bacterium suspension by using the gun head, sequentially adding the spore bacterium suspension on each glass slide, and uniformly blowing and beating the glass slides containing the nano copper oxide to respectively prepare the spore bacterium suspension containing the nano copper oxide. And (3) placing the glass slide into a climatic incubator for culturing at 26 ℃, taking out the concave slide after culturing for 4h, and placing the concave slide under a 10X 40 times optical microscope to observe the spore germination condition.
The obtained results are shown in figure 3, the spore germination of phytophthora is inhibited to different degrees in 6.25, 12.5, 25 and 50mg/L compared with the control, the inhibition is enhanced along with the increase of the concentration, and the germination rate of 100mg/L treatment is 37.74 percent and is obviously lower than 90.35 percent of the control. The pretreated nano copper oxide has an inhibiting effect on phytophthora nicotianae spore germination.
Example 6 determination of antibacterial Activity of Phytophthora nicotianae Nano copper oxide antibacterial agent
The phytophthora nicotianae nano copper oxide antibacterial agent is prepared according to the steps of example 2, wherein the concentrations and the masses of the nano copper oxide and the metalaxyl manganese zinc are shown in table 1. mu.L of each of the phytophthora nicotianae nano-copper oxide antimicrobial agents was added to the corresponding melted OA medium of the same formulation, and a cake (6mm) obtained from the edge of a 3-day-old fungus culture was placed in the center of each dish. All samples were then incubated at 28 ℃ in the dark and the colony diameter was measured after 3 d.
TABLE 1 synergistic antibacterial activity of nano copper oxide and metalaxyl manganese zinc on phytophthora nicotianae
As shown in Table 1, according to the analysis of the actually measured inhibition rate of phytophthora nicotianae, the mixing mass ratio of metalaxyl manganese zinc and pretreated nano copper oxide in the phytophthora nicotianae nano copper oxide antibacterial agent is (25-100):1, and the synergistic bactericidal effect is achieved, wherein the nano copper oxide accounts for 25mg/L and the metalaxyl manganese zinc accounts for 0.5mg/L, the mass ratio is 50:1, and the synergistic factor is 1.51.
Example 7: experimental determination of inhibiting endocytosis
The phytophthora nicotianae nano copper oxide antibacterial agent prepared in the example 1 (the concentration of the nano copper oxide is 25mg/L) is sprayed on the surface of tobacco leaves infected with phytophthora nicotianae, the tobacco leaves are sprayed 2 times a day, 5ml of the tobacco leaves are sprayed each time, 10 days of the spraying are continuously sprayed, the spraying is repeated for 3 times, and the average content of the nano copper oxide in the tobacco leaves is 8mg/L through determination.
The same batch of nano copper oxide which is not pretreated is adopted in the control group, the antibacterial agent is prepared by the same preparation steps of the example 1 (the concentration of the nano copper oxide is 25mg/L), other experimental conditions are unchanged, the operation is repeated for 3 times, and the average content of the nano copper oxide in the tobacco leaves is determined to be 75 mg/L.
Therefore, the endocytosis between the tobacco leaf cells and the nano copper oxide can be weakened through the pretreatment of the nano copper oxide, and the accumulation of the nano copper oxide in the plant body is further reduced.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (9)
1. The phytophthora nicotianae nano copper oxide antibacterial agent is characterized in that the main components of the nano copper oxide antibacterial agent are nano copper oxide and metalaxyl manganese zinc, and the mass ratio of the nano copper oxide to the metalaxyl manganese zinc is (25-200): 1.
2. The phytophthora nicotianae nano-copper oxide antibacterial agent of claim 1, wherein the nano-copper oxide has a particle size of 20-100 nm.
3. The phytophthora nicotianae nano-copper oxide antibacterial agent of claim 2, wherein the concentration of the nano-copper oxide in the phytophthora nicotianae nano-copper oxide antibacterial agent is 25mg/L to 100 mg/L.
4. The phytophthora nicotianae nano copper oxide antibacterial agent according to claim 3, wherein the concentration of metalaxyl manganese zinc in the phytophthora nicotianae nano copper oxide antibacterial agent is 0.25mg/L to 1 mg/L.
5. The phytophthora nicotianae nano copper oxide antibacterial agent according to claim 4, wherein in the phytophthora nicotianae nano copper oxide antibacterial agent, the concentration of nano copper oxide is 25mg/L, the concentration of metalaxyl manganese zinc is 0.5mg/L, and the mass ratio is 50: 1.
6. The method for preparing the phytophthora nicotianae nano copper oxide antibacterial agent according to claim 5, characterized by comprising the following steps:
(1) pre-treating nano copper oxide, namely dispersing the nano copper oxide in 75-80 w% ethanol solution, introducing direct current, taking out after 20-30min, adding 25 wt% of methyl- β -cyclodextrin solution, dispersing for 30-45min in an ultrasonic instrument with the frequency of 35kHz, heating in a water bath until the water content is lower than 20 wt%, drying by using nitrogen, and irradiating for 5min by using ultraviolet rays to obtain the pre-treated nano copper oxide;
(2) preparing an antibacterial agent: adding the pretreated nano copper oxide into ultrapure water, adjusting the pH value to be 6-7, adding arabic gum, stirring, heating in a water bath to 35-45 ℃, stopping heating after the arabic gum is dissolved, naturally cooling, adding metalaxyl manganese zinc, and uniformly stirring to obtain the antibacterial agent.
7. The method for preparing the phytophthora nicotianae nano copper oxide antibacterial agent according to claim 6, wherein in the step (1), the direct current voltage is 10-12V, and the electrodes are all copper sheets.
8. The method for preparing the phytophthora nicotianae nano copper oxide antibacterial agent according to claim 7, wherein in the step (1), the weight ratio of the nano copper oxide to the methyl- β -cyclodextrin solution is 1: 1.
9. The method for preparing the phytophthora nicotianae nano copper oxide antibacterial agent according to claim 8, wherein in the step (2), the mass ratio of the arabic gum to the nano copper oxide is 0.01: 4.
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