CN109042734A - A kind of nano antibacterial agent for preventing and treating fungal diseases of plants - Google Patents
A kind of nano antibacterial agent for preventing and treating fungal diseases of plants Download PDFInfo
- Publication number
- CN109042734A CN109042734A CN201810832450.5A CN201810832450A CN109042734A CN 109042734 A CN109042734 A CN 109042734A CN 201810832450 A CN201810832450 A CN 201810832450A CN 109042734 A CN109042734 A CN 109042734A
- Authority
- CN
- China
- Prior art keywords
- nano
- antibacterial agent
- suspension
- cupric oxide
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of nano antibacterial agents for preventing and treating fungal diseases of plants, are made of nano cupric oxide and the suspension that water is formed, and wherein the concentration of nano cupric oxide is 50-200mg/L.High-efficiency plant antibacterial agent prepared by the present invention is promoting plant to the resistance of pathogen, inhibits fungal infection plant leaf blade to have remarkable efficacy, can effectively prevent, fungi is inhibited to infect plant leaf blade.
Description
Technical field
The invention belongs to technical field of plant protection, in particular to a kind of high-efficiency plant antifungal agent.More particularly to one kind
Apply to prevent and treat the nano antibacterial agent that fungi infects plant, includes its main component, preparation method and its method of administration.
Background technique
The fungal disease of plant is all to influence the important disease of agricultural production all the time.Currently, the fungal disease of plant is complete
70% or more of disease sum has been accounted within the scope of ball, it is even more widely distributed in China, such as: rot disease, anthracnose, wheel line
Disease, powdery mildew, diplostomiasis, dry rot etc..Influence of the fungal disease for crops through agricultural product production, product transport, storage,
Links are sold, main harm, which is embodied in, reduces crop yield, quality, influences production estimation safety etc..
Include at present chemical prevention for the main preventive and control measure of fungal diseases of plants, biological control, improve crop
The methods of kind.As efficient sterilizing methods, antifungal agent is widely used in agricultural disease prevention and control field, such as triazole type
Fungicide, the Ion pairings such as carbendazim, Bordeaux mixture etc..But these organic medicaments occur largely anti-in prevention and treatment
Medicine bacterial strain, in addition organic medicament is big to non-target organism toxicity, some not degradable pesticides or organic metabolites in environment or
Animal and plant body accumulation.
Nano material typically refers to material of the scale within the scope of 1~100nm, since it is with small-size effect, quantum
The characteristics such as dimensional effect, skin effect, bulk effect, in mechanics, photoelectricity, microbiology, material science is biomedical, ring
Have wide application prospects and huge application potential in the various fields such as border protection and reparation.
Summary of the invention
The purpose of the present invention is to provide a kind of with the novel nano antibacterial agent for inhibiting fungal infection plant, includes its master
Want ingredient, preparation method and its method of administration.
The important component of the novel plant antifungal agent involved in the present invention arrived is nano cupric oxide.Nano cupric oxide is changed
Formula is CuO, and crystal form is spherical shape, and color is in black.Cupric oxide nano-rod has skin effect, quantum size effect, volume effect
It should be widely used in being catalyzed, sensor with the characteristics such as macro quanta tunnel effect, the side such as photoelectron material and environment remediation
Face.The desulfurization effect of nano cupric oxide is best in all poly-metal deoxides, and which are mainly applied to the fields such as bituminous coal desulfurization.It is real
It tests and shows that the nano cupric oxide of different structure has a stronger catalytic action to Performance for Decomposition of Ammonium Perchlorate reaction, and porous copper oxide
Nanometer rods are to potassium chlorate and hydrogen peroxide catalytic activity with higher.In sensor production, nano cupric oxide can increase
Sensitivity to predetermined substance, such as: nano cupric oxide is applied to H2O2The sensor of detection, CO gas concentration inductor etc..
The present invention relates to nano cupric oxide hinder the growth of mycelia from the sprouting for inhibiting fungal spore, promoted and anti-
Pathogen infection three angles of relevant plant endogenous hormones level inhibit infection processs of the fungi on plant leaf blade, are promoted
The ability of plant resistance to fungal disease.
Specific technical solution of the present invention is as follows:
A kind of nano antibacterial agent for preventing and treating fungal diseases of plants, is made of nano cupric oxide and the suspension that water is formed,
The concentration of middle nano cupric oxide is 50-200mg/L.
Under preferable case, the partial size of nano cupric oxide is 30-50nm, and zeta current potential is -12.70mV, and conductivity is
0.0513mS/cm, solubility is 627 μ g/L (pH=7.0), purity > 99.9% in water.Experiment shows the nano oxygen of the physical property
It is significant to the killing effect of fungi to change copper.
Under preferable case, ammonium hydroxide can also be contained in nano antibacterial agent of the invention, volume of the ammonium hydroxide in suspension is dense
Degree is 5-10%.The present invention is found through experiments that, ammonium hydroxide is added in the suspension of nano cupric oxide, by nanometer of the invention
When antibacterial agent is sprayed on plant surface, it is capable of forming local ammonia atmosphere, effective starvation, to remarkably promote nano oxygen
Change copper to the killing effect of fungi；Furthermore the suspension sprayed can be absorbed by plants because containing ammonium hydroxide as nitrogenous fertilizer, can also be risen
To the effect of plant nutrition liquid.
Under preferable case, the vegetable oil of 5-10% volume can also be added in nano antibacterial agent of the invention.Utilize plant
Oil improves the hydrophobicity of leaves of plants table with the affinity of leaves of plants table, to improve nano cupric oxide in the attachment of leaves of plants table
And enrichment.Vegetable oil includes but is not limited to soybean oil, various oil of plant.In further preferred situation, vegetable oil, which uses, to be had
The Chinese herbal medicine extract oil of bacteriostasis efficacy, such as Atractylis oil, cassia twig oil or medicine Anacamptis pyromaidalis oil, a small amount of of these Chinese herbal medicine extract oils add
Enter, improving, leaves of plants table is hydrophobic simultaneously, moreover it is possible to synergistic effect is formed with nano cupric oxide, to be formed inorganic and organic
Fungicide it is compound, fungi killing effect than single nano copper oxide anti-bacteria agent improve about 15% or so.
Under preferable case, the surfactant of 2-5% volume can also be added in nano antibacterial agent of the invention.Pass through table
Face activating agent improves the dispersion performances of the fungicide such as nano cupric oxide, and raising sprays efficiency.Surfactant preferably uses coconut palm
Oleamide DEA, sodium laureth sulfate, TEA lauryl sodium sulfate, Tea Saponin and combinations thereof.
According to another aspect of the present invention, a kind of preparation side of nano antibacterial agent for preventing and treating fungal diseases of plants is also provided
Method, comprising the following steps:
(1) using copper nitrate and ammonium hydroxide as raw material, in autoclave using hydrothermal reduction legal system obtain hydroxide deposits and
Reaction solution；
(2) it after being filtered, washed and dried hydroxide deposits, is placed into microwave agglomerating furnace and is rapidly heated to 600-
800 DEG C of temperature, heat preservation calcining 20-30 minutes grind after cooling and obtain nano-cupric oxide powder；
(3) obtained nano-cupric oxide powder is configured in the reaction solution in step (1), is sufficiently stirred that form nanometer anti-
Bacterium suspension.
Under preferable case, the cleaning solution formed in step (2) is also added in the reaction solution formed in step (1), jointly
Lysate as nano-cupric oxide powder in step (3).
In above-mentioned preparation method, using ammonium hydroxide as reaction dissolvent, the effect of reducing agent is not only acted as；The reaction solution of formation and
The waste liquids later period such as cleaning solution, not only non-wastewater discharge, was also used as simultaneously directly as the lysate of nano-cupric oxide powder
Plant nitrogen fertilizer application, so that nano antibacterial agent obtained can sterilize and can be to plant getting fat.
In addition, in order to obtain the nano cupric oxide that physical parameter is met the requirements, the present invention carries out preparation process condition
Further preferably, wherein hydrothermal process condition is preferably 180-300 DEG C in step (1), and pressure maintaining 2-4 hours.It is used in step (2)
Microwave fast heating (rate of heat addition is 30-50 DEG C/mi n), to prevent the crystal grain of molybdena crystal from growing up.Pass through above-mentioned work
The diameter of the strict control of skill, final obtained nano cupric oxide is 30-50nm, and zeta current potential is -12.70mV, and conductivity is
0.0513mS/cm, solubility is 627 μ g/L (pH=7.0), purity > 99.9% in water.
Under preferable case, the plant of proper ratio is optionally added in the nano-antibacterial suspension made from step (3)
Object oil and surfactant, to further increase the comprehensive performance of nano antibacterial agent.
Antibacterial agent is directly sprayed on plant leaf blade surface by the method for administration of nano antibacterial agent of the invention.
The present invention is based on the outbursts of fungal disease to the bring serious challenge of China's agricultural production, and to nano oxidized
The understanding of copper antimicrobial effect invents a kind of novel plant antifungal agent to reduce the outburst of fungal disease, suppression by adjusting concentration
Infection processs of the fungi processed on plant leaf blade, alleviate fungal infection damaged caused by plant, have biggish feasibility and
Huge application potential.
Detailed description of the invention
Fig. 1 show embodiment 1 on the blade of fungal infection in spray antibacterial agent 10 days of various concentration after, fungi
Infect effect.
Behind antibacterial agent 10 days for spraying various concentration during Fig. 2 shows embodiments 1 on the blade of fungal infection, leaves of plants
Piece fungi bacterial plaque area.
Fig. 3 show embodiment 2 on the blade of fungal infection in spray antibacterial agent 10 days of various concentration after, scanning electricity
Mirror home position observation effect.
Fig. 4 show embodiment 3 on the leaf of fungal infection in spray antibacterial agent 10 days of various concentration after, blade interplantation
The situation of change of object hormone concentration.
Specific embodiment
Below by a kind of specific embodiment antibacterial with inhibition fungal infection plant that the present invention will be described in detail is related to
Agent and its application method, the embodiment are suitable only for more specifically introducing the invention in detail, but should not be understood as to appoint
What form limitation present invention.
Embodiment 1
The nano antibacterial agent of preparation prevention and treatment fungal diseases of plants, comprising the following steps:
(1) industrial ammonia (concentration 28%) for weighing 500g copper nitrate and 1L is added in autoclave, is added 1L and is gone
Ionized water dilution.It is heated to 220 DEG C in closed situation, reacts 3 hours.
(2) mixed liquor after reaction is filtered, obtains hydroxide deposits and reaction solution, Kocide SD is precipitated
After object washing and drying, it is placed into microwave agglomerating furnace and is rapidly heated with 40 DEG C/mi n to 600 DEG C of temperature, 30 points of heat preservation calcining
Clock grinds after cooling and obtains nano-cupric oxide powder.
(3) obtained nano-cupric oxide powder is configured in the reaction solution in step (1), be sufficiently stirred to be formed it is certain dense
The nano cupric oxide suspension of degree.
(4) the TEA dodecyl sulphate of 3% volume is separately added into the nano cupric oxide suspension made from step (3)
The rhizoma atractylodis extract oil of sodium and 5% volume.
Embodiment 2
The present embodiment is for illustrating that antibacterial agent according to the present invention infects plant leaf blade inhibiting effect to fungal pathogens.
This experiment is ' Samantha Sha ' (Samantha) using Chinese rose (Rose hybrida) kind, by Changping District, Beijing south
Mouth farm flower cultivation base obtains.Clip 4-5 saves the spray that every section has 5 pinnate compound leaves, is immediately placed in clear water, in 2h
Take back laboratory treatment.Spray trims base portion in water, retain be put into distilled water after 3-4 piece pinnate compound leaf balance it is spare.This
Testing used bacterial strain is Sphaerotheca pannosa, Podosphaera sac fungus (Podosphaera pannosa, P.pannosa),
It is provided by Gardening College, China Agriculture Univ..
20mL sterile water is added in 50ml centrifuge tube in the dense Chinese rose blade of clip powdery mildew, and vortex instrument fullys shake
Go to spare in 10mL centrifuge tube after removing blade afterwards, concentration is adjusted to 1 × 106A/mL obtains certain density powdery mildew spores
Suspension shakes up, and every 4ml is fitted into 10ml centrifuge tube, carries out liquid level scale mark, and 7000rpm, 15min discard supernatant liquid,
The nano antibacterial agent of respective concentration is added to mark line, forms powdery mildew-nano antibacterial agent mixed liquor.
4g agar powder is accurately weighed, distilled water is settled to 1L, 121 DEG C of high pressure sterilization 20min.It is configured to 0.4% water agar
Culture medium (1L).
Moisturizing object is obtained by pouring into batch cultur ware after prepared 0.4% water agar autoclave sterilization, with beating
Blade is broken into disk by hole device, its blade back is spread in upward on solidified water agar, then with spray-on process by the white powder of respective concentration
Bacterium-nano antibacterial agent mixed liquor, is sprayed on blade surface, to melon infected with powdery mildew fungus plant leaf blade.
After the completion of inoculation, blade is transferred to artificial climate incubator, is cultivated 48 hours under dark condition, then at 16 hours
Illumination/8 hour dark alternation condition is continuously cultivated 8 days.Statistics, measurement incidence after the completion of infecting.
The measurement of bacterial plaque area: it after 10 days of infection, photographs to record blade bacterial plaque and infects area, use software image J
Count different disposal blade bacterial plaque area.
Data processing: all experiments are respectively provided with 3 Duplicate Samples, average value are calculated using Excel, using SPSS19 to experiment
Data carry out single factor test variance (ANOVA) analysis, and wherein Duncan analyzes conspicuousness variation (p≤0.05) between experimental data.
The results show that the nano copper oxide anti-bacteria agent of two kinds of concentration inhibits Powdery Mildew invading on Chinese rose blade
Dye.Compared with space management, the nano copper oxide anti-bacteria agent of two kinds of concentration significantly inhibits incidence (Fig. 1 of Powdery Mildew
And Fig. 2).Show efficient antibacterial effect.
Embodiment 3
The experimentation of the present embodiment is substantially the same manner as Example 2, and difference is, after infection processs, collects fungi
Blade is infected, using scanning electron microscope (SEM, Phenom ProX, Phenom, NLD) observation mycelia in blade quantity, upgrowth situation.
The results show that new antifungal agents of the present invention inhibit the sprouting and growth of Powdery Mildew, two kinds of concentration
Antibacterial agent inhibit the quantity and vigor of Powdery Mildew.Transmission electron microscope shows that Powdery Mildew mycelia is dense in space management,
It is sturdy, it is vibrant；After the antimycotic treatment of two kinds of concentration, there is only a small amount of Powdery Mildew mycelia on blade face, and mycelia is in fibre
Thin shape, debility (Fig. 3).Microexamination further proves that the antifungal agent bacterium of two kinds of concentration inhibits Powdery Mildew to blade
Infect, influence of the antibacterial agent to mycelia is especially apparent.
Embodiment 4
The experimentation of the present embodiment is substantially the same manner as Example 2, and difference is, after infection processs, collects different
Handle the blade infected.The blade being stored in -20 DEG C of low temperature refrigerator after weighing 1g liquid nitrogen flash freezer with mortar, adds 2mL sample
Product extracting solution is ground into homogenate under condition of ice bath, is transferred to 10mL test tube, then by several times rinsed well mortar with 2mL extracting solution,
It is transferred in test tube, is placed on after shaking up in 4 DEG C of refrigerators together.4h is extracted at 4 DEG C, 1000g is centrifuged 15min, takes supernatant.Add
1mL extracting solution is sink in shallow lake, is mixed, and condition extracts 1h again at 4 DEG C, centrifugation, is merged supernatant and is recorded volume, residue is abandoned
It goes.Using enzyme-linked immunization (ELISA, enzyme linked immunosorbent assay), plant hormone zeatin is measured
The variation of nucleosides (ZR, zeatin-riboside) concentration.
The results show that novel plant antibacterial agent of the present invention plays external source under conditions of melon infected with powdery mildew fungus
The effect of stimulation improves the concentration of blade implants hormone ZR, and then improves the resistance that plant against fungal infects, it is suppressed that
Infection processs (Fig. 4) of the Powdery Mildew on blade.
Claims (8)
1. a kind of nano antibacterial agent for preventing and treating fungal diseases of plants, which is characterized in that the suspension formed by nano cupric oxide and water
Liquid is constituted, and wherein the concentration of nano cupric oxide is 50-200mg/L.
2. nano antibacterial agent according to claim 1, which is characterized in that wherein also contain ammonium hydroxide in suspension, ammonium hydroxide exists
Volumetric concentration in suspension is 5-10%.
3. nano antibacterial agent according to claim 1, which is characterized in that wherein also containing 5-10% volume in suspension
Vegetable oil.
4. nano antibacterial agent according to claim 1, which is characterized in that wherein also containing 2-5% volume in suspension
Surfactant.
5. a kind of preparation method for the nano antibacterial agent for preventing and treating fungal diseases of plants, which comprises the following steps:
(1) using copper nitrate and ammonium hydroxide as raw material, hydroxide deposits and reaction are obtained using hydrothermal reduction legal system in autoclave
Liquid；
(2) it after being filtered, washed and dried hydroxide deposits, is placed into microwave agglomerating furnace and is rapidly heated to 600-800
DEG C temperature, heat preservation calcining 20-30 minute, it is cooling after grinding acquisition nano-cupric oxide powder；
(3) obtained nano-cupric oxide powder is configured in the reaction solution in step (1), is sufficiently stirred that form nano-antibacterial outstanding
Supernatant liquid.
6. preparation method according to claim 5, which is characterized in that 5-10% body is added in the suspension of step (3)
Long-pending vegetable oil.
7. preparation method according to claim 5, which is characterized in that 2-5% volume is added in the suspension of step (3)
Surfactant.
8. preparation method according to claim 5, which is characterized in that the cleaning solution wherein formed in step (2) is also added
Into the reaction solution formed in step (1), collectively as the lysate of nano-cupric oxide powder in step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810832450.5A CN109042734A (en) | 2018-07-26 | 2018-07-26 | A kind of nano antibacterial agent for preventing and treating fungal diseases of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810832450.5A CN109042734A (en) | 2018-07-26 | 2018-07-26 | A kind of nano antibacterial agent for preventing and treating fungal diseases of plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109042734A true CN109042734A (en) | 2018-12-21 |
Family
ID=64835845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810832450.5A Pending CN109042734A (en) | 2018-07-26 | 2018-07-26 | A kind of nano antibacterial agent for preventing and treating fungal diseases of plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109042734A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810438A (en) * | 2019-09-29 | 2020-02-21 | 西南大学 | Phytophthora nicotianae nano copper oxide antibacterial agent and preparation method thereof |
| CN113287634A (en) * | 2021-06-09 | 2021-08-24 | 江南大学 | Method for controlling tomato wilt based on nano elemental sulfur |
| CN113498781A (en) * | 2021-08-16 | 2021-10-15 | 中国农业大学 | Application of nano manganese dioxide in preventing and treating bacterial leaf blight of rice |
| CN113875464A (en) * | 2021-09-27 | 2022-01-04 | 广西绿循环新材料技术有限责任公司 | Method for biologically preventing and treating aniseed anthracnose by combining nano antibacterial agent |
| CN115894128A (en) * | 2022-12-15 | 2023-04-04 | 中国热带农业科学院南亚热带作物研究所 | Foliar fertilizer for preventing and treating pineapple heart rot |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194629A (en) * | 2006-12-06 | 2008-06-11 | 山东科技大学 | Solid phase synthesis method for nano curpic hydroxide fungicide powder |
| CN101273723A (en) * | 2008-05-16 | 2008-10-01 | 曲阜师范大学 | Method for preparing nano copper oxide anti-bacteria agent |
| CN103712896A (en) * | 2013-11-20 | 2014-04-09 | 天津商业大学 | Method for determining nano-fluid suspension stability |
-
2018
- 2018-07-26 CN CN201810832450.5A patent/CN109042734A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194629A (en) * | 2006-12-06 | 2008-06-11 | 山东科技大学 | Solid phase synthesis method for nano curpic hydroxide fungicide powder |
| CN101273723A (en) * | 2008-05-16 | 2008-10-01 | 曲阜师范大学 | Method for preparing nano copper oxide anti-bacteria agent |
| CN103712896A (en) * | 2013-11-20 | 2014-04-09 | 天津商业大学 | Method for determining nano-fluid suspension stability |
Non-Patent Citations (5)
| Title |
|---|
| 刘娟 等: "纳米氧化铜球的水热法合成与表征", 《应用化工》 * |
| 张凯: "CuO纳米颗粒对鲤鱼的致毒效应及在鲤鱼体内的分布", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 徐立娜 等: "CuO纳米颗粒对拟南芥叶片生长及生理特性的影响", 《植物生理学报》 * |
| 董汇泽 等: "纳米CuO对几种蔬菜病菌的抑制作用", 《北方园艺》 * |
| 褚天铎 等: "《简明施肥技术手册 第2版》", 31 January 2014, 金盾出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810438A (en) * | 2019-09-29 | 2020-02-21 | 西南大学 | Phytophthora nicotianae nano copper oxide antibacterial agent and preparation method thereof |
| CN113287634A (en) * | 2021-06-09 | 2021-08-24 | 江南大学 | Method for controlling tomato wilt based on nano elemental sulfur |
| CN113287634B (en) * | 2021-06-09 | 2022-02-01 | 江南大学 | Method for controlling tomato wilt based on nano elemental sulfur |
| CN113498781A (en) * | 2021-08-16 | 2021-10-15 | 中国农业大学 | Application of nano manganese dioxide in preventing and treating bacterial leaf blight of rice |
| CN113875464A (en) * | 2021-09-27 | 2022-01-04 | 广西绿循环新材料技术有限责任公司 | Method for biologically preventing and treating aniseed anthracnose by combining nano antibacterial agent |
| CN115894128A (en) * | 2022-12-15 | 2023-04-04 | 中国热带农业科学院南亚热带作物研究所 | Foliar fertilizer for preventing and treating pineapple heart rot |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109042734A (en) | A kind of nano antibacterial agent for preventing and treating fungal diseases of plants | |
| KR102124574B1 (en) | Method for producing natural complex composition having prevention of pests, offensive odor removal of barn, and sterilization effect | |
| CN102578116B (en) | Compounded botanical synergistic fungicide containing sophocarpidine and osthol and application for preparation of compounded botanical synergistic fungicide | |
| CN109844095B (en) | Aspergillus niger F22 strain having nematicidal activity against plant parasitic nematodes and use thereof | |
| CN103300069A (en) | Biological bactericide composition and application thereof to wilt | |
| CN102919256A (en) | Fluopicolide-containing compound bactericide and application thereof | |
| CN101658179A (en) | Complex bactericidal composition containing cyazofamid | |
| CN103518750A (en) | High-efficiency compound biological bactericide and application thereof | |
| CN102223802A (en) | Plant extracts for use as phytochemicals | |
| El-Batal et al. | Protective role of iron oxide nanocomposites on disease index, and biochemical resistance indicators against Fusarium oxysporum induced-cucumber wilt disease: In vitro, and in vivo studies | |
| CN102948424A (en) | Boscalid-containing bactericidal composition | |
| CN101647451A (en) | Sterilization combination containing cyazofamid | |
| Karmous et al. | Biologically synthesized zinc and copper oxide nanoparticles using Cannabis sativa L. enhance soybean (Glycine max) defense against Fusarium virguliforme | |
| CN101697725A (en) | Pesticide composition containing protective bactericide | |
| CN107573143A (en) | A kind of environment-friendly type peanut seed coat agent | |
| Soubeih et al. | Comparative studies using nanotechnology on fungal Diseases defense to productivity improvement of squash crop | |
| CN1994083B (en) | A compound bactericidal agent | |
| CN114617127A (en) | Pesticide sterilization composition and application thereof | |
| CN114703098A (en) | Fermentation preparation and application of Bacillus belgii fungicide for preventing and treating cotton wilt | |
| CN103548849A (en) | Synergistic compound biological bactericide and application thereof | |
| CN107410315A (en) | Bactericidal composition containing kasugarnycin and terpenol | |
| Karimovna et al. | Efficiency of nanopolymer application on the growth, development and yield of soybean. | |
| CN103081920A (en) | Sterilizing composition containing penconazole | |
| Taimooz | Behavior of some nanomaterials in improving the growth of onion plant, Allium cepa and its effect on Pythium aphanidermatum. | |
| Saritha et al. | Allelopathic effects of Celosia argentea L Leaf extracts on crop plant seed germination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181221 |