CN110776113A - Aquaculture compound microbial preparation and preparation method thereof - Google Patents
Aquaculture compound microbial preparation and preparation method thereof Download PDFInfo
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- CN110776113A CN110776113A CN201911179690.0A CN201911179690A CN110776113A CN 110776113 A CN110776113 A CN 110776113A CN 201911179690 A CN201911179690 A CN 201911179690A CN 110776113 A CN110776113 A CN 110776113A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 59
- 230000000813 microbial effect Effects 0.000 title claims abstract description 41
- 238000009360 aquaculture Methods 0.000 title claims abstract description 37
- 244000144974 aquaculture Species 0.000 title claims abstract description 37
- 150000001875 compounds Chemical class 0.000 title claims abstract description 32
- 239000000203 mixture Substances 0.000 claims abstract description 42
- 239000002994 raw material Substances 0.000 claims abstract description 35
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 32
- 241000233838 Commelina Species 0.000 claims abstract description 28
- 235000003434 Sesamum indicum Nutrition 0.000 claims abstract description 27
- 235000012054 meals Nutrition 0.000 claims abstract description 27
- 244000269722 Thea sinensis Species 0.000 claims abstract 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 241000207961 Sesamum Species 0.000 claims description 26
- 238000003756 stirring Methods 0.000 claims description 22
- 244000063299 Bacillus subtilis Species 0.000 claims description 17
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 17
- 108010059892 Cellulase Proteins 0.000 claims description 17
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 17
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 17
- 244000057717 Streptococcus lactis Species 0.000 claims description 17
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 17
- 229940106157 cellulase Drugs 0.000 claims description 17
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 14
- 239000011259 mixed solution Substances 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 238000002791 soaking Methods 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 239000000292 calcium oxide Substances 0.000 claims description 7
- 235000012255 calcium oxide Nutrition 0.000 claims description 7
- 238000011049 filling Methods 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 102000010911 Enzyme Precursors Human genes 0.000 claims description 2
- 108010062466 Enzyme Precursors Proteins 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims 7
- 241000238424 Crustacea Species 0.000 abstract description 12
- 241001465754 Metazoa Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 4
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 230000002180 anti-stress Effects 0.000 abstract description 3
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 abstract description 2
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 abstract description 2
- 210000004204 blood vessel Anatomy 0.000 abstract description 2
- 102000038379 digestive enzymes Human genes 0.000 abstract description 2
- 108091007734 digestive enzymes Proteins 0.000 abstract description 2
- 210000002472 endoplasmic reticulum Anatomy 0.000 abstract description 2
- 229960002442 glucosamine Drugs 0.000 abstract description 2
- 239000002366 mineral element Substances 0.000 abstract description 2
- 210000003205 muscle Anatomy 0.000 abstract description 2
- 210000005036 nerve Anatomy 0.000 abstract description 2
- 230000028327 secretion Effects 0.000 abstract description 2
- 210000002460 smooth muscle Anatomy 0.000 abstract description 2
- 241000143060 Americamysis bahia Species 0.000 abstract 1
- 244000000231 Sesamum indicum Species 0.000 abstract 1
- 241000238557 Decapoda Species 0.000 description 27
- 241001122767 Theaceae Species 0.000 description 24
- 229920002101 Chitin Polymers 0.000 description 6
- 241000223782 Ciliophora Species 0.000 description 5
- 238000007873 sieving Methods 0.000 description 5
- 239000000049 pigment Substances 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 159000000007 calcium salts Chemical class 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 3
- 229930182490 saponin Natural products 0.000 description 3
- 150000007949 saponins Chemical class 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000238553 Litopenaeus vannamei Species 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 208000002720 Malnutrition Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
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- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
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- 235000000824 malnutrition Nutrition 0.000 description 2
- -1 nitrogen-containing polysaccharide Chemical class 0.000 description 2
- 208000015380 nutritional deficiency disease Diseases 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000233839 Commelina communis Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- UPEZCKBFRMILAV-JNEQICEOSA-N Ecdysone Natural products O=C1[C@H]2[C@@](C)([C@@H]3C([C@@]4(O)[C@@](C)([C@H]([C@H]([C@@H](O)CCC(O)(C)C)C)CC4)CC3)=C1)C[C@H](O)[C@H](O)C2 UPEZCKBFRMILAV-JNEQICEOSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001622452 Sphaeranthus Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000005276 aerator Methods 0.000 description 1
- UPEZCKBFRMILAV-UHFFFAOYSA-N alpha-Ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C(O)CCC(C)(C)O)C)CCC33O)C)C3=CC(=O)C21 UPEZCKBFRMILAV-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- UPEZCKBFRMILAV-JMZLNJERSA-N ecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@H]([C@H](O)CCC(C)(C)O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 UPEZCKBFRMILAV-JMZLNJERSA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 108060003552 hemocyanin Proteins 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 235000001705 insufficient nutrition Nutrition 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000031586 molting cycle Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Hydrology & Water Resources (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Engineering & Computer Science (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention provides an aquaculture compound microbial preparation and a preparation method thereof, belonging to the field of biochemistry. The aquaculture compound microbial preparation provided by the invention comprises the following raw materials in percentage by weight: 15-30% of tea seed cake, 10-30% of sesame seed meal, 10-30% of brown sugar, 2-3% of enzyme-bacterium mixture, 5-15% of dayflower and 15-30% of lime water. The aquaculture compound microbial preparation provided by the invention can promote the secretion of digestive enzymes in shrimps, stimulate the rapid running of glucosamine to form a new shell, accelerate the effective absorption of mineral elements, inhibit the release of calcium ions from smooth muscle endoplasmic reticulum, reduce the concentration of calcium ions in cells, excite central nerves, expand blood vessels, increase the muscle contractility, improve the anti-stress and anti-osmotic pressure effects, and thus can stimulate the crustacean aquatic animals to smoothly exuviate.
Description
Technical Field
The invention relates to an aquaculture compound microbial preparation and a preparation method thereof, belonging to the field of biochemistry.
Background
The molting of crustaceans is not consistent with the exoskeleton construction of growing crustaceans, e.g. the branchipes are uncalcified, flexible, thin layers of crust, whereas the decapod are firm, thick shells, structurally significantly different. Generally, the organic material contained in the outer skin is a nitrogen-containing polysaccharide chitin, which is relatively soft and flexible, but the outer skin must also be strengthened by deposition and nitration of calcium salts. The skin of the ten-foot eye can be divided into four main parts, namely an epidermis, a pigment layer, a calcification layer and a membranous layer. The epidermis is composed of proteins and lipids and has a relatively uniform texture. The main role of this layer is related to permeability, with no chitin found, but sometimes calcified. The pigment layer is below the surface, is a calcified chitin layer, but also contains proteins, and is hexagonal in shape when viewed from the surface, with a central region of calcium-salt-filled cylindrical chitin, and the proteins are concentrated in the regions between the cylinders, which contain pigment particles. The calcified layer is a chitin layer containing calcium salts beneath the pigment layer, and it accounts for a large portion of the total exoskeleton thickness. The uncalcified chitin layer on the subcutaneous cells is the membrane layer.
The growth and development of crustaceans is always associated with molting, the entire process of molting involves the removal of the old crustacean, the individual rapidly increases due to water absorption, and then a new crustacean is formed and hardened. Therefore, the individual growth of the crustacean is not continuous in appearance and is stepped, and one step is formed after each skin is sloughed once. Specifically, the molting cycle can be divided into molt, post-molt, meta-molt and pre-molt.
The failure of shelling in current shrimp farming is a big killer in the shrimp farming community. Once the shells cannot be removed or the shelling is prolonged, the prawns are extremely easy to die. Shelling is a key issue in the growth and health of shrimp life activities. The smooth shelling directly affects the growth rate and survival rate, and they can be caused by the physiological reaction inside and the change of the external environment in the growth process, such as water quality deterioration, malnutrition, excessive density, strong illumination, water temperature mutation, medicine and other influences.
There is no good preparation prepared from natural products in the prior art for helping crustacean aquatic animals to rapidly exuviate.
Disclosure of Invention
The invention provides a compound microbial preparation for promoting quick shelling of shellfish aquatic animals.
Specifically, the invention is realized by the following technical scheme:
the invention provides an aquaculture compound microbial preparation, which comprises the following raw materials in percentage by weight:
15-30% of tea seed cake, 10-30% of sesame seed meal, 10-30% of brown sugar, 2-3% of enzyme-bacterium mixture, 5-15% of dayflower and 15-30% of lime water.
Preferably, the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
10-20 parts of bacillus natto, 15-30 parts of saccharomyces cerevisiae, 10-30 parts of cellulase and 10-20 parts of lactococcus lactis.
Preferably, the preparation method of the lime water comprises the following steps:
uniformly mixing 1-1.2 parts of water and 1 part of quicklime by weight, stirring for 5-20 minutes, standing for 1-3 hours, and taking supernatant to obtain the calcium carbonate.
Preferably, the aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
Preferably, the aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
10 parts of bacillus natto, 30 parts of saccharomyces cerevisiae, 15 parts of cellulase and 15 parts of lactococcus lactis.
Preferably, the aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
20% of tea seed cake, 15% of sesame seed meal, 24% of brown sugar, 3% of zymogen mixture, 13% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
Preferably, the aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
15% of tea seed cake, 30% of sesame seed meal, 15% of brown sugar, 3% of enzyme-bacterium mixture, 12% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
Preferably, the aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
27% of tea seed cake, 10% of sesame seed meal, 25% of brown sugar, 3% of enzyme-bacterium mixture, 15% of dayflower and 20% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
Secondly, the invention also provides a preparation method of the aquaculture compound microbial preparation, which comprises the following steps:
s1, crushing and mixing the tea seed cake, the sesame seed meal and the dayflower, adding water, soaking for 24-48 hours, and intermittently stirring during the soaking period to obtain a mixed solution;
s2, adding the enzyme-bacterium mixture and brown sugar into the mixed solution, continuously filling air into the system for 18-36 hours, and sealing for 60-84 hours to obtain fermentation liquor;
and S3, adding lime water into the fermentation liquor, and uniformly stirring to obtain the product.
Preferably, the addition amount of the water is 8-12 times of the sum of the weight of the tea seed cake, the sesame seed meal and the dayflower.
The invention has the advantages that: the microbial preparation provided by the invention comprises tea seed cakes and dayflower. The tea seed cake contains tea saponin, and is soaked in water under stirring to remove part of toxin. The tea saponin has destructive effect on animal erythrocyte, can produce hemolysis, and has no obvious influence on leukocyte. The crustacean is mostly hemocyanin cells, and a small amount of tea saponin has obvious stimulation and shell-removing effects on the crustacean. The tea seed cake contains crude protein, fat, phosphoric acid and various trace elements, such as C/N29.8%, nitrogen 1.44%, phosphorus 0.28%, potassium 1.18%, calcium 0.2%, magnesium 0.17%, copper 10.7%, zinc 26.7mg/kg, and also iron, boron, copper, etc., which are various elements required after the crustacean is taken off the shell. The Commelina communis belongs to the genus Sphaeranthus, contains ecdysone, has no toxicity and inflammation, clears away heat and toxic materials, can fight harmful parasites of organisms, accelerate growth, promote metabolism and in vivo protein synthesis, and enhance anti-stress capability, and is a natural object which has no toxic or side effect and can be obtained conveniently.
Therefore, the product contains a large amount of various vitamins and trace elements required by the shelled shrimps, promotes the secretion of digestive enzymes in the shrimps, stimulates the rapid running of glucosamine to form a new shell, accelerates the effective absorption of mineral elements, inhibits the release of calcium ions from smooth muscle endoplasmic reticulum, reduces the concentration of calcium ions in cells, excites central nerves, expands blood vessels, increases muscle contractility, improves the anti-stress and anti-osmotic pressure effects, and can stimulate the crustacean aquatic animals to smoothly shell.
Detailed Description
The present invention is further illustrated by the following detailed description of specific embodiments, which are provided for the purpose of illustration only and are not to be construed as further limiting.
Example 1
An aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
The preparation method of the lime water comprises the following steps:
and (2) uniformly mixing 1 part of water and 1 part of quicklime in parts by weight, stirring for 15 minutes, standing for 3 hours, and taking supernatant to obtain the calcium carbonate.
The preparation method of the aquaculture compound microbial preparation provided by the embodiment comprises the following steps:
s1, crushing and mixing 30 parts of tea seed cakes, 18 parts of sesame seed meal and 15 parts of dayflower in parts by weight, sieving with a 100-mesh sieve, adding 630 parts of water, soaking for 36 hours, and stirring for 10 minutes every 8 hours during soaking to obtain a mixed solution;
s2, adding 2 parts of enzyme-bacterium mixture and 10 parts of brown sugar into the mixed solution by weight, continuously filling air into the system for 24 hours, and sealing for 72 hours to obtain fermentation liquor;
s3, adding 25 parts by weight of lime water into the fermentation liquor, and uniformly stirring to obtain the product.
Example 2
An aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
10 parts of bacillus natto, 30 parts of saccharomyces cerevisiae, 15 parts of cellulase and 15 parts of lactococcus lactis.
The preparation method of the lime water comprises the following steps:
and (2) uniformly mixing 1 part of water and 1 part of quicklime in parts by weight, stirring for 15 minutes, standing for 3 hours, and taking supernatant to obtain the calcium carbonate.
The preparation method of the aquaculture compound microbial preparation provided by the embodiment comprises the following steps:
s1, crushing and mixing 30 parts of tea seed cakes, 18 parts of sesame seed meal and 15 parts of dayflower in parts by weight, sieving with a 100-mesh sieve, adding 630 parts of water, soaking for 36 hours, and stirring for 10 minutes every 8 hours during soaking to obtain a mixed solution;
s2, adding 2 parts of enzyme-bacterium mixture and 10 parts of brown sugar into the mixed solution by weight, continuously filling air into the system for 24 hours, and sealing for 72 hours to obtain fermentation liquor;
s3, adding 25 parts by weight of lime water into the fermentation liquor, and uniformly stirring to obtain the product.
Example 3
An aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
20% of tea seed cake, 15% of sesame seed meal, 24% of brown sugar, 3% of enzyme-bacterium mixture, 13% of dayflower and 25% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
The preparation method of the lime water comprises the following steps:
and (2) uniformly mixing 1 part of water and 1 part of quicklime in parts by weight, stirring for 15 minutes, standing for 3 hours, and taking supernatant to obtain the calcium carbonate.
The preparation method of the aquaculture compound microbial preparation provided by the embodiment comprises the following steps:
s1, crushing and mixing 20 parts of tea seed cakes, 15 parts of sesame seed meal and 13 parts of dayflower in parts by weight, sieving with a 100-mesh sieve, adding 480 parts of water, soaking for 36 hours, and stirring for 10 minutes every 8 hours during soaking to obtain a mixed solution;
s2, adding 3 parts of enzyme-bacterium mixture and 24 parts of brown sugar into the mixed solution by weight, continuously filling air into the system for 24 hours, and sealing for 72 hours to obtain fermentation liquor;
s3, adding 25 parts by weight of lime water into the fermentation liquor, and uniformly stirring to obtain the product.
Example 4
An aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
15% of tea seed cake, 30% of sesame seed meal, 15% of brown sugar, 3% of enzyme-bacterium mixture, 12% of dayflower and 25% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
The preparation method of the lime water comprises the following steps:
and (2) uniformly mixing 1 part of water and 1 part of quicklime in parts by weight, stirring for 15 minutes, standing for 3 hours, and taking supernatant to obtain the calcium carbonate.
The preparation method of the aquaculture compound microbial preparation provided by the embodiment comprises the following steps:
s1, crushing and mixing 15 parts of tea seed cakes, 30 parts of sesame seed meal and 12 parts of dayflower in parts by weight, sieving with a 100-mesh sieve, adding 470 parts of water, soaking for 36 hours, and stirring for 10 minutes every 8 hours during soaking to obtain a mixed solution;
s2, adding 3 parts of enzyme-bacterium mixture and 15 parts of brown sugar into the mixed solution by weight, continuously filling air into the system for 24 hours, and sealing for 72 hours to obtain fermentation liquor;
s3, adding 25 parts by weight of lime water into the fermentation liquor, and uniformly stirring to obtain the product.
Example 5
An aquaculture compound microbial preparation comprises the following raw materials in percentage by weight:
27% of tea seed cake, 10% of sesame seed meal, 25% of brown sugar, 3% of enzyme-bacterium mixture, 15% of dayflower and 20% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
The preparation method of the lime water comprises the following steps:
and (2) uniformly mixing 1 part of water and 1 part of quicklime in parts by weight, stirring for 15 minutes, standing for 3 hours, and taking supernatant to obtain the calcium carbonate.
The preparation method of the aquaculture compound microbial preparation provided by the embodiment comprises the following steps:
s1, crushing and mixing 27 parts of tea seed cakes, 10 parts of sesame seed meal and 15 parts of dayflower in parts by weight, sieving with a 100-mesh sieve, adding 520 parts of water, soaking for 36 hours, and stirring for 10 minutes every 8 hours during soaking to obtain a mixed solution;
s2, adding 3 parts of enzyme-bacterium mixture and 25 parts of brown sugar into the mixed solution by weight, continuously filling air into the system for 24 hours, and sealing for 72 hours to obtain fermentation liquor;
and S3, adding 20 parts by weight of lime water into the fermentation liquor, and uniformly stirring to obtain the calcium carbonate fermentation liquor.
Test example 1
The prawn breeding pond is located beside a large red-ridge sluice in a pearl sea sluice area, the pond mainly grows prawns in 3 mu of soil pond in yellow-Da manner, 22 ten thousand of prawn seedlings are put in the pond in total, when the breeding period is 42 days, the prawns grow to 5 to 7 centimeters, but the body forms are thinned, the water quality is fresh and transparent, the water temperature is 28 ℃, the pH value is 8.2, the ammonia nitrogen and the nitrite are 0, meanwhile, the prawns are slowly eaten, a small amount of ciliates and naematococcus are seen during microscopic examination, and the total feed amount is 400 jin.
The judgment is that the compound microbial preparation for aquaculture provided by the embodiment 1 of the invention can not be shelled due to the insufficient nutrition caused by the insufficient feeding and the influence of long-term hunger, and then the compound microbial preparation for aquaculture is used for 4 jin per mu for two days. On the third day the shrimp began to shell, but the shell size was not much. After 5 days, the net is used once again and is increased to 8 jin per mu, a large amount of prawn head shells appear in the garbage lift net under the aerator every other day, and the shrimps begin to eat a large amount of food.
Test example 2
The method is characterized in that the penaeus vannamei is cultivated in a 5-mu soil pond of Mr. Zhao, which is a large trench in the Zhuhai sluice gate area, 30 thousands of shrimps are thrown, the temperature suddenly drops by 5 ℃ after the typhoon, the penaeus vannamei stops eating, the water body is turbid, the pH value is 8.0, the concentration of nitrite is 0.2 mg/L, and the concentration of ammonia nitrogen is 0.2 mg/L. Microscopic examination shows that the organic matter is much, a small amount of green algae and diatom, a large amount of ciliates, the surface of shrimp bodies is rough, and the shrimp tails have polypide and ciliates.
By using the aquaculture compound microbial preparation provided by the embodiment 2 of the invention, 8 jin per mu can be used for 2 days continuously, so that the trace elements and vitamin C can be supplemented to the shells of the shrimps. After the fishing net is used in the next day, the water surface and the garbage fishing net have already appeared to have a head shell, and in the third day, a large amount of whole body shells emerge on the water surface to replace a large amount of head shells. And (4) microscopic examination is carried out on the third day, the ciliates basically disappear, the prawns begin to eat, and the prawns are not hindered after one week.
Test example 3
A large trench sluice gate type culturing house located in the pearl sea sluice gate area is old, a 900 cubic soil film pond is used for culturing 20 thousands of prawns, the prawns are yellow in body color when cultured for 35 days, the body length is about 3 to 9 centimeters, the size differentiation is serious, the food intake is slow, and the food consumption is 0.3 to 1 jin each day. The supernatant of the water body is turbid, the detected PH value is 8.3, the concentrations of nitrite and ammonia nitrogen are 0.1 milligram per liter and 0.15 milligram per liter, the concentration of calcium is 140 milligram per liter, the concentration of magnesium is 107 milligram per liter, and the concentration of potassium is 57 milligram per liter. A small amount of ciliates and naked algae are found under microscopic examination, the water temperature is 30 ℃, the bodies of the shrimps are white, meanwhile, black spots are found on the bodies of the shrimps, and the frontal swords are broken, so that the shrimps are judged to be caused by overlarge density and malnutrition.
The aquaculture compound microbial preparation provided by the embodiment 3 of the invention is used for 3 times according to the standard of 10 jin of the feed amount per mu, is discharged from a bottom sewage discharge pipe after the compound microbial preparation is exposed to the shell, is added with fresh water and various trace elements and is fed by fresh insects, and is fed back to a sewage discharge outlet to discharge a large amount of shrimp shells after 5 days, and meanwhile, the compound microbial preparation is fresh and smooth in color and vitality to the shrimp bodies and is increased in food intake.
Claims (10)
1. An aquaculture compound microbial preparation is characterized by comprising the following raw materials in percentage by weight:
15-30% of tea seed cake, 10-30% of sesame seed meal, 10-30% of brown sugar, 2-3% of enzyme-bacterium mixture, 5-15% of dayflower and 15-30% of lime water.
2. The aquaculture composite microbial preparation of claim 1, wherein the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
10-20 parts of bacillus natto, 15-30 parts of saccharomyces cerevisiae, 10-30 parts of cellulase and 10-20 parts of lactococcus lactis.
3. The aquaculture composite microbial preparation of claim 1, wherein the preparation method of the lime water comprises the following steps:
uniformly mixing 1-1.2 parts of water and 1 part of quicklime by weight, stirring for 5-20 minutes, standing for 1-3 hours, and taking supernatant to obtain the calcium carbonate.
4. The aquaculture composite microbial preparation according to claim 2, comprising the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
5. The aquaculture composite microbial preparation according to claim 2, comprising the following raw materials in percentage by weight:
30% of tea seed cake, 18% of sesame seed meal, 10% of brown sugar, 2% of enzyme-bacterium mixture, 15% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
10 parts of bacillus natto, 30 parts of saccharomyces cerevisiae, 15 parts of cellulase and 15 parts of lactococcus lactis.
6. The aquaculture composite microbial preparation according to claim 2, comprising the following raw materials in percentage by weight:
20% of tea seed cake, 15% of sesame seed meal, 24% of brown sugar, 3% of zymogen mixture, 13% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
7. The aquaculture composite microbial preparation according to claim 2, comprising the following raw materials in percentage by weight:
15% of tea seed cake, 30% of sesame seed meal, 15% of brown sugar, 3% of enzyme-bacterium mixture, 12% of dayflower and 25% of lime water;
the enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
8. The aquaculture composite microbial preparation according to claim 2, comprising the following raw materials in percentage by weight:
27% of tea seed cake, 10% of sesame seed meal, 25% of brown sugar, 3% of enzyme-bacterium mixture, 15% of dayflower and 20% of lime water.
The enzyme-bacterium mixture comprises the following raw materials in parts by weight:
15 parts of bacillus natto, 20 parts of saccharomyces cerevisiae, 20 parts of cellulase and 12 parts of lactococcus lactis.
9. A method of producing an aquaculture complex microbial preparation according to any of claims 1 to 3, comprising the steps of:
s1, crushing and mixing the tea seed cake, the sesame seed meal and the dayflower, adding water, soaking for 24-48 hours, and intermittently stirring during the soaking period to obtain a mixed solution;
s2, adding the enzyme-bacterium mixture and brown sugar into the mixed solution, continuously filling air into the system for 18-36 hours, and sealing for 60-84 hours to obtain fermentation liquor;
and S3, adding lime water into the fermentation liquor, and uniformly stirring to obtain the product.
10. The method for preparing the compound microbial preparation for aquaculture according to claim 9, wherein the addition amount of the water is 8-12 times of the sum of the mass of the tea seed cake, the mass of the sesame seed meal and the mass of the dayflower.
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