CN110756499A - Cleaning device, liquid discharge device and corresponding cleaning method - Google Patents
Cleaning device, liquid discharge device and corresponding cleaning method Download PDFInfo
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- CN110756499A CN110756499A CN201810837563.4A CN201810837563A CN110756499A CN 110756499 A CN110756499 A CN 110756499A CN 201810837563 A CN201810837563 A CN 201810837563A CN 110756499 A CN110756499 A CN 110756499A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
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Abstract
The application discloses a cleaning device, a liquid discharge device and a corresponding cleaning method. The liquid discharge unit comprises a liquid discharge needle for extracting waste liquid; the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline so as to provide extraction power for the liquid discharge needle; and the isolation chamber is arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump and is matched with the extraction pump to adjust the extraction power provided for the liquid discharge needle. In this way, this application can simple and conveniently buffer adjustment twitch power, can obtain lower magnetic bead loss rate, and is convenient for maintain the liquid way device.
Description
Technical Field
The application relates to the technical field of liquid paths, in particular to a cleaning device, a liquid drainage device and a corresponding cleaning method.
Background
The immunomagnetic bead separation technology is a new separation technology combining the high specificity of immunology and the specific magnetic responsiveness of magnetic beads, is an immunology detection method and an antigen purification means with strong specificity and high sensitivity, and is widely applied to the aspects of cell separation, protein detection, immunology detection, microbiological detection and the like.
In order to separate the non-target substance in a free state from the target substance combined with the magnetic beads, the magnetic separation and cleaning of the reactant are required in a magnetic field, the cleaning process is completed by repeatedly and circularly extracting supernatant waste liquid in a reaction cup in the magnetic field and then re-injecting cleaning liquid, and the loss rate of the target magnetic beads needs to be controlled within a certain range in the cleaning process so as to provide enough compounds meeting the detection requirement. In the process of extracting the supernatant liquid waste, target magnetic beads are usually taken away due to overlarge negative pressure, so that the final testing process is influenced. At present, two methods are generally adopted for extracting supernatant liquid: firstly, the negative pressure is controlled by an injector, and supernatant waste liquid is sucked into a corresponding waste liquid pipe through a liquid discharge needle; and secondly, connecting a liquid discharge needle by using a peristaltic pump, and directly pumping the supernatant into a waste liquid pool.
The inventor of the application finds that the process of controlling the negative pressure to suck the supernatant is complex and the movement space of the component is increased in the first scheme; the tube of the peristaltic pump of the second scheme needs to be replaced frequently, which is not beneficial to maintenance and influences user experience.
Disclosure of Invention
The technical problem that this application mainly solved provides a belt cleaning device, drain and cleaning method, can buffer the regulation twitch power simply conveniently, can obtain lower magnetic bead loss rate, and be convenient for maintain the liquid way device.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a cleaning device including: a liquid discharge unit comprising: a liquid discharge needle for drawing waste liquid; the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline so as to provide extraction power for the liquid discharge needle; and the isolation chamber is arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump and is matched with the extraction pump to adjust the extraction power provided for the liquid discharge needle.
In order to solve the above technical problem, another technical solution adopted by the present application is: a drain, comprising: a liquid discharge needle for extracting waste liquid; the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline to provide extraction power for the liquid discharge needle; and the isolation chamber is arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump and is matched with the extraction pump to adjust the extraction power provided for the liquid discharge needle.
In order to solve the above technical problem, another technical solution adopted by the present application is: there is provided a cleaning method comprising: placing a reaction container below a liquid discharge needle, and controlling the liquid discharge needle to move downwards to a proper position of the reaction container; starting a diaphragm pump, and adjusting the duty ratio parameter of the diaphragm pump so as to discharge the supernatant in the reaction container through the liquid discharge needle, the isolation chamber and the diaphragm pump; switching a first electromagnetic valve to a normally closed end, and fully operating the isolation pump to evacuate residual liquid in the isolation chamber; rotating the reaction container to the position below the liquid injection needle, and starting a plunger pump to inject the cleaning liquid in the cleaning liquid supplier into the reaction container through the liquid injection needle; and rotating the reaction container to a position below the liquid discharge needle, and repeating the steps to finish the cleaning and liquid discharge processes for a preset number of times.
The beneficial effect of this application is: different from the situation of the prior art, the cleaning device, the liquid discharging device and the cleaning method can utilize the isolation chamber to buffer and regulate the negative pressure extraction power, so that the extraction force of the liquid discharging needle is controlled within a certain range, the phenomenon of mistakenly extracting target magnetic beads is effectively avoided, and the loss rate of the magnetic beads is ensured to meet the performance test requirement; compared with a peristaltic pump as a power source for extracting power under negative pressure, the diaphragm pump does not need to be frequently replaced, so that the service life is longer, and better maintenance is facilitated; the plunger pump is used as an input power source of the cleaning liquid, the cleaning liquid injected into the reaction cup can be accurately controlled, and meanwhile, the plunger pump is small in size and beneficial to realizing modular management of instruments.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic diagram of an embodiment of a drain of the present application;
FIG. 2 is a schematic structural view of an embodiment of the cleaning apparatus of the present application;
FIG. 3 is a schematic flow chart of an embodiment of the cleaning method of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a liquid discharge apparatus 100 according to the present application, the liquid discharge apparatus 100 is used for discharging a waste liquid in a reaction vessel, for example, a supernatant in a reaction cup used in a magnetic separation and washing process of a reactant in an immunomagnetic bead separation technology, and the liquid discharge apparatus 100 includes a liquid discharge needle 110, an extraction pump 120, and an isolation chamber 130.
In the present embodiment, the drainage device 100 may be an independent liquid path device, or may be a liquid path unit in any equipment, for example, a liquid path unit of a washing device in a blood cell analyzer, which may be used in combination with other liquid path units in the washing device, for example, a washing unit for injecting a washing liquid, to complete the whole washing process.
Specifically, the drainage needle 110 of the drainage device 100 is used to be inserted into a reaction vessel to extract waste liquid in the reaction vessel, such as supernatant in a reaction cup. The suction pump 120 is connected to the drainage needle 110 through a drainage line 140 to provide suction power, such as negative pressure, to the drainage needle 110. And a compartment 130 is provided on the drain line 140 between the drain needle 110 and the draw pump 120 to cooperate with the draw pump 120 to regulate the pumping power buffered to the drain needle 110.
In this embodiment, the extraction pump 120 may be a diaphragm pump, which does not require frequent replacement of the associated tubing, and which has a longer life and facilitates better access for maintenance than a peristaltic pump. The specific structural design of the drainage pipe 140 may be determined according to practical applications, and is not limited herein. The isolation chamber 130 and the diaphragm pump 120 are disposed on the drain line 140, and the isolation chamber 130 is connected to the diaphragm pump 120, so that the isolation chamber 130 can play a role of buffering the negative pressure pumping power when the diaphragm pump 120 adjusts the pumping power of the negative pressure. The diaphragm pump 120 may also be referred to as a control pump, which receives a control signal from a controller or computer during a control process to change the flow rate of the fluid and maintain a tuning parameter (e.g., duty cycle parameter) within a desired range, thereby automating the manufacturing process.
In addition, the drainage device 100 may further include a first solenoid valve 150, such as a two-position three-way solenoid valve, in which a closed chamber is provided, through holes are opened at different positions, each hole connects different passages, a piston is in the middle of the chamber, two electromagnets are provided at both sides, and which magnet coil is energized to which side the valve body is attracted, so that it can open or close different through holes by controlling the movement of the valve body. That is, the two-position three-way electromagnetic valve is controlled by double coils, one coil is powered on instantly, then the power supply is closed, the valve is opened, and the corresponding channels are communicated with each other; the other coil is instantaneously energized and then the power supply is turned off, the valve is closed, and the corresponding passages are communicated with each other. The two-position three-way electromagnetic valve can be kept in a closed or opened state for a long time, so that the service life of the coil is longer, and the two-position three-way electromagnetic valve is best used in a high-temperature pipeline.
In the present embodiment, as shown in fig. 1, the isolation chamber 130 is provided with a first opening 131, a second opening 132 and a third opening 133, wherein the first opening 131 is provided at the upper end of the sidewall of the isolation chamber 130, the second opening 132 is provided at the lower end of the sidewall of the isolation chamber 130, and the third opening 133 is provided at the top end of the isolation chamber 130. The normally open end 150A of the first solenoid valve 150 is connected to the first opening 131 of the isolation chamber 130 through the drain line 140, the normally closed end 150B of the first solenoid valve 150 is connected to the second opening 132 of the isolation chamber 130 through the drain line 140, and the working end 150C of the first solenoid valve 150 is connected to the draw pump 120 through the drain line 140; and the third opening 133 of the isolation chamber 130 is connected to the drainage needle 110 through the drainage line 140.
In the present embodiment, the diaphragm pump 120 can cooperate with the first solenoid valve 150 to adjust the negative pressure suction force input into the liquid discharge device 100, and when the first solenoid valve 150 is switched to the normally open end 150A and opened, the diaphragm pump 120 is connected to the first opening 131 of the isolation chamber 130 to provide the suction force for the normal operation of the liquid discharge needle 110 to draw the supernatant in the reaction cup; when the first solenoid valve 150 is switched to the normally closed end 150B and opened, the diaphragm pump 120 is connected to the second opening 132 of the isolation chamber 130, and the waste liquid accumulated in the isolation chamber 130 can be extracted. In the whole liquid discharging process, the isolation chamber 130 can buffer and regulate the negative pressure extraction power input into the liquid discharging device 100, so that the extraction force in the liquid discharging needle 110 is not too strong, and the target magnetic beads in the reaction cup are extracted while the liquid discharging needle 110 extracts the supernatant in the reaction cup.
Further, as shown in fig. 1, the drainage device 100 further includes a waste liquid tank 160, and the waste liquid tank 160 is disposed at the end of the drainage pipe 140 and connected to the diaphragm pump 120 through the drainage pipe 140. Therefore, the waste liquid pumped and discharged by the drainage device 100 can be injected into the waste liquid barrel 160 through the relevant passage.
Referring to fig. 2, fig. 2 is a schematic structural diagram of an embodiment of a cleaning apparatus according to the present application. As shown in fig. 2, the cleaning apparatus 200 of the present embodiment includes a liquid discharge unit 210 and a cleaning unit 220.
As will be understood by those skilled in the art, the drainage unit 210 in this embodiment is similar to the drainage device 100 shown in fig. 1, and includes a drainage needle 211, a suction pump 212, an isolation chamber 213, a first solenoid valve 214, a waste liquid tank 215, and a drainage pipeline 216 connecting the above components. The various components of the drainage unit 210 are similar to the drainage device 100 shown in fig. 1 and therefore will not be described in detail herein.
The cleaning unit 220 is used to provide a new cleaning solution to the reaction vessel. That is, after the waste liquid (supernatant) in the reaction vessel is discharged by the liquid discharge unit 210, a new cleaning liquid is further injected into the reaction vessel by the cleaning unit 220 to clean the target objects (e.g., target magnetic beads) in the reaction vessel again, and the waste liquid is discharged by the liquid discharge unit 210 after the cleaning, so that the target objects can be cleaned for a plurality of times.
Specifically, the cleaning unit 220 includes a cleaning liquid supplier 221, a liquid injection needle 222, and a plunger pump 223. Wherein, the cleaning solution supplier 221 is used for accommodating and supplying the cleaning solution; the liquid injection needle 222 is connected to the cleaning liquid supplier 221 through a liquid injection line 225 to obtain the cleaning liquid from the cleaning liquid supplier 221 and supply the cleaning liquid to the reaction vessel; the plunger pump 223 is disposed on the liquid injection line 225 and connected to the cleaning liquid supplier 221 and the liquid injection needle 222 to provide a power source to deliver the cleaning liquid in the cleaning liquid supplier 221 to the liquid injection needle 222.
In addition, the cleaning unit 220 may further include a second solenoid valve 224, for example, a two-position three-way solenoid valve. Wherein the working end of the second electromagnetic valve 224 is connected to the plunger pump 223, the normally open end of the second electromagnetic valve 224 is connected to the liquid injection line 225, and the normally closed end of the second electromagnetic valve 224 is connected to the cleaning liquid supply 221. Therefore, the second electromagnetic valve 224 cooperates with the plunger pump 223 to deliver the cleaning liquid in the cleaning liquid supplier 221 to the liquid injection needle 222 by the plunger pump 223 according to the demand.
Further, as shown in fig. 2, in the present embodiment, the liquid discharge needle 211 in the liquid discharge unit 210 may include a long needle 2111 and a short needle 2112 arranged side by side, wherein the long needle 2111 is connected to the isolation chamber 213 and the extraction pump 212 through the liquid discharge line 216 to extract the waste liquid, i.e., to perform the liquid discharge function. And the short needle 2112 is disposed adjacent to the long needle 2111 and connected to the liquid injection line 225 in the cleaning unit 220 to take a cleaning liquid from the cleaning unit 220 to perform outer wall cleaning of the long needle 2111.
Specifically, in the present embodiment, the cleaning unit 220 may further include a third solenoid valve 226 provided in the liquid injection line 225 between the second solenoid valve 224 and the liquid injection needle 222. The third solenoid valve 226 may be a two-position three-way solenoid valve, the working end of the third solenoid valve 226 is connected to the liquid injection line 225, the normally open end of the third solenoid valve 226 is connected to the liquid injection needle 222, and the normally closed end of the third solenoid valve 226 is connected to the short needle 2112. That is, it is possible to control whether the cleaning liquid is injected into the injection needle 222 by the third solenoid valve 226 to add the cleaning liquid to the reaction cup or to control the cleaning liquid to be injected into the short needle 2112 to clean the outer wall of the long needle 2111.
Referring to fig. 3, fig. 3 is a schematic flow chart of an embodiment of the cleaning method of the present application, which is suitable for the cleaning apparatus shown in fig. 2, and the detailed description of the related contents refers to the above cleaning apparatus, which is not described herein again. The method comprises the following steps:
step S101: the reaction vessel is placed under the drain needle 211, and the drain needle 211 is controlled to move down to a proper position of the reaction vessel.
Step S102: the diaphragm pump 212 is turned on, and the duty parameter of the diaphragm pump 212 is adjusted so that the supernatant in the reaction vessel is discharged through the liquid discharge needle 211, the isolation chamber 213, and the diaphragm pump 212, and further discharged to the waste liquid tank 215.
Step S103: switching the first solenoid valve 214 to the normally closed end, fully operating the isolation pump 212 to evacuate the residual liquid in the isolation chamber 213;
step S104: the reaction vessel is rotated to below the injection needle 222, and the plunger pump 223 is turned on to inject the cleaning solution in the cleaning solution supplier 221 into the reaction vessel through the injection needle 222.
Step S105: the reaction vessel is rotated to the position below the drain needle 211, and the above steps are repeated to complete the cleaning and draining processes for a predetermined number of times.
Step S106: after the predetermined number of times of cleaning is completed, the plunger pump 223, the diaphragm pump 212, and the third electromagnetic valve 226 are turned on to complete the cleaning of the liquid discharge needle 211.
In summary, by the cleaning device and the cleaning method, 1) the isolation chamber can be used for buffering and adjusting the negative pressure extraction power, so that the extraction force of the drainage needle is controlled within a certain range, the phenomenon of mistakenly extracting target magnetic beads is effectively avoided, and the loss rate of the magnetic beads is ensured to meet the performance test requirement; 2) compared with a peristaltic pump as a power source for extracting power under negative pressure, the diaphragm pump does not need to be frequently replaced, so that the service life is longer, and better maintenance is facilitated; 3) the plunger pump is used as an input power source of the cleaning liquid, the cleaning liquid injected into the reaction cup can be accurately controlled, and meanwhile, the plunger pump is small in size and beneficial to realizing modular management of instruments.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (10)
1. A cleaning device, comprising:
a liquid discharge unit for discharging a waste liquid in the reaction vessel, wherein the liquid discharge unit includes:
a liquid discharge needle for extracting waste liquid;
the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline so as to provide extraction power for the liquid discharge needle;
an isolation chamber arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump,
to cooperate with the draw pump to buffer the draw power provided to the weep needle.
2. The cleaning apparatus defined in claim 1, wherein the extraction pump is a diaphragm pump.
3. The cleaning device of claim 1, wherein the drainage unit further comprises:
and the first electromagnetic valve is arranged on a liquid discharge pipeline between the isolation chamber and the diaphragm pump.
4. The cleaning device according to claim 3, wherein the first solenoid valve is a two-position three-way solenoid valve, and the isolation chamber is provided with a first opening, a second opening and a third opening, wherein the first opening is provided at an upper end of a side wall of the isolation chamber, the second opening is provided at a lower end of the side wall of the isolation chamber, and the third opening is provided at a top end of the isolation chamber, a normally open end of the first solenoid valve is connected to the first opening of the isolation chamber through a drain line, a normally closed end of the first solenoid valve is connected to the second opening of the isolation chamber through a drain line, and a working end of the first solenoid valve is connected to the suction pump through a drain line; and the third opening of the isolation chamber is connected to the drainage needle through a drainage line.
5. The cleaning device of claim 1, further comprising:
a cleaning unit for providing a cleaning solution to the reaction vessel, wherein the cleaning unit comprises:
a cleaning liquid supplier for receiving and supplying a cleaning liquid;
the liquid injection needle is connected with the cleaning liquid supplier through a liquid injection pipeline so as to obtain the cleaning liquid from the cleaning liquid supplier and provide the cleaning liquid to the reaction container;
and the plunger pump is arranged on the liquid injection pipeline and is connected with the cleaning liquid supplier and the liquid injection needle so as to provide a power source for conveying the cleaning liquid in the cleaning liquid supplier to the liquid injection needle.
6. The cleaning device of claim 5, wherein the cleaning unit further comprises:
and the working end of the second electromagnetic valve is connected with the plunger pump, the normally open end of the second electromagnetic valve is connected with the liquid injection pipeline, and the normally closed end of the second electromagnetic valve is connected with the cleaning liquid supplier.
7. The cleaning device of claim 6, wherein the drainage needle comprises a long needle and a short needle arranged side by side, wherein the long needle is connected to the isolation chamber and the extraction pump through a drainage line to extract waste liquid; and the short needle is connected with the liquid injection pipeline to obtain cleaning liquid to clean the long needle.
8. The cleaning device of claim 7, wherein the cleaning unit further comprises:
and the working end of the third electromagnetic valve is connected with the liquid injection pipeline, the normally open end of the third electromagnetic valve is connected with the liquid injection needle, and the normally closed end of the third electromagnetic valve is connected with the short needle.
9. A drain, comprising:
a liquid discharge needle for extracting waste liquid;
the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline to provide extraction power for the liquid discharge needle;
and the isolation chamber is arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump and is matched with the extraction pump to adjust the extraction power provided for the liquid discharge needle.
10. A method of cleaning, comprising:
placing a reaction container below a liquid discharge needle, and controlling the liquid discharge needle to move downwards to a proper position of the reaction container;
starting a diaphragm pump, and adjusting the duty ratio parameter of the diaphragm pump so as to discharge the supernatant in the reaction container through the liquid discharge needle, the isolation chamber and the diaphragm pump;
switching a first electromagnetic valve to a normally closed end, and fully operating the isolation pump to evacuate residual liquid in the isolation chamber;
rotating the reaction container to the position below the liquid injection needle, and starting a plunger pump to inject the cleaning liquid in the cleaning liquid supplier into the reaction container through the liquid injection needle;
and rotating the reaction container to a position below the liquid discharge needle, and repeating the steps to finish the cleaning and liquid discharge processes for a preset number of times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810837563.4A CN110756499A (en) | 2018-07-26 | 2018-07-26 | Cleaning device, liquid discharge device and corresponding cleaning method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810837563.4A CN110756499A (en) | 2018-07-26 | 2018-07-26 | Cleaning device, liquid discharge device and corresponding cleaning method |
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| CN110756499A true CN110756499A (en) | 2020-02-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810837563.4A Pending CN110756499A (en) | 2018-07-26 | 2018-07-26 | Cleaning device, liquid discharge device and corresponding cleaning method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113275342A (en) * | 2021-06-22 | 2021-08-20 | 宙斯生命科技(常州)有限公司 | Reaction cup washing mechanism and reaction cup washing method |
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