Background technology
For Full-automatic chemiluminescence immunoassay analysis meter, the paramagnetic particles of Sheet clonal antibody and sample to be measured are added in reaction cup, antigen in sample to be measured is combined with the antibody on paramagnetic particles surface, add the antibody of alkali phosphatase enzyme mark again, solid-phase coating antibody-antigene-enzymic-labelled antibody compound is formed after incubation, at elect magnetic field, 4 washings are carried out to this compound, unconjugated unnecessary antigen and alkali phosphatase enzyme mark antibody are washed away, add under substrate luminous agent is combined in the catalysis of the alkaline phosphatase on magnetic particle surface more again, rapid dephosphorization acid gene, generate unstable mediator AMPD.AMPD decomposes very soon, gets back to low-energy stable state, launch photon simultaneously from high energy excited state, and this chemiluminescence continues and stablizes, can for a few hours.By optical quantum counter system log (SYSLOG) luminous intensity, and calculate the concentration of determined antigen from typical curve.At cleaning station, the magnetic bead adding cleaning fluid is cleaned, adsorb after carry out the absorption of reaction cup cleaning fluid, the magnetic bead now wrapping quilt is attracted to magnet place, inhales to wash pin and decline and enter into reaction cup, draws the cleaning fluid in reaction cup, retains the magnetic bead of bag quilt.
In actual applications, the magnetic bead of bag quilt generally adopts permanent magnet to adsorb, due to magnetic field of magnets intensity there are differences, there is the reason such as gap and magnet assembling in reaction cup and magnet surface, magnetic bead is caused can not to be effectively adsorbed on the reaction cup inwall of magnet, need the speed controlling to draw reaction cup cleaning fluid, bag could be reduced by the loss of magnetic bead.
At present, on instrument similar at home and abroad, adopt the mode of direct current peristaltic pump and stepper motor peristaltic pump more, adopting problem main is in this way, direct current peristaltic pump operating rate control method is complicated, the bad control of speed; Although stepper motor peristaltic pump can control operating rate, but many pump heads peristaltic pump only has a stepper motor, although the speed drawing reaction cup cleaning fluid can control, but the speed of each pump head is consistent, can not regulate separately, when running into fluid path water resistance and being uneven, cause the actual capture velocity of each reaction cup cleaning fluid inconsistent, increase scavenging period.
Utility model content
The utility model embodiment provides a kind of chemical illumination immunity analysis instrument reaction cup cleaning fluid rate of liquid aspiration regulating device, so that the cleaning fluid rate of liquid aspiration of each reaction cup can be regulated respectively, while guaranteeing that lost package is not by magnetic bead, when running into fluid path water resistance and being uneven, can regulate the cleaning fluid rate of liquid aspiration of single reaction cup, to reduce scavenging period.This device comprises: pin is washed in multiple suction, multiple flexible pipe, multiple membrane pump, speed-regulating control circuit, each described suction is washed pin and is contacted with the cleaning fluid in a reaction cup, each described suction is washed pin and is connected with a described membrane pump by a described flexible pipe, multiple described membrane pump is all connected with described speed-regulating control circuit, wherein, described speed-regulating control circuit, for output multi-channel waveform signal, trigger by every road waveform signal the described membrane pump be connected with described speed-regulating control circuit and regulate operating rate, wherein, described Multichannel Waveform signal and described multiple membrane pump one_to_one corresponding, described membrane pump, washes pin for adopting the operating rate after adjustment by described suction and draws cleaning fluid in reaction cup.
In one embodiment, described speed-regulating control circuit, specifically for output multi-channel composite wave-shape signal, triggers by pulsed frequency and the change of pulse width of every road composite wave-shape signal the described membrane pump be connected with described speed-regulating control circuit and regulates operating rate.
In one embodiment, described speed-regulating control circuit becomes large specifically for the pulse width by modulating composite wave-shape signal and the pulsed frequency of modulation composite wave-shape signal diminishes, and triggers the described membrane pump be connected with described speed-regulating control circuit and reduces operating rate.
In one embodiment, described speed-regulating control circuit comprises: pulse width modulated waveform generator, for generation of multiplex pulse wide modulation waveform signal and multichannel low frequency square-wave signal; Pulse modulated circuit, for by multiplex pulse wide modulation waveform signal Zhong mono-tunnel pulse width modulated waveform signal and multichannel low frequency square-wave signal Zhong mono-tunnel low frequency square-wave signal, be modulated to a road composite wave-shape signal, to form multichannel composite wave-shape signal; Light lotus root buffer circuit, the input end of described smooth lotus root buffer circuit is connected with the output terminal of described pulse modulated circuit, the described smooth output terminal of lotus root buffer circuit is connected with the control end of membrane pump, for carrying out the isolation of light lotus root to multichannel composite wave-shape signal, and the multichannel composite wave-shape signal after being isolated by light lotus root exports to membrane pump respectively.
In one embodiment, also comprise: external circuit, for receiving the speed parameter of the operating rate of described membrane pump; Described speed-regulating control circuit, comprising: communicating circuit, for the speed parameter of the multiple described membrane pump be connected with described speed-regulating control circuit is sent to described external circuit.
In one embodiment, pin is washed in described suction, comprising: minute hand, and the front end of described minute hand and the liquid level of cleaning fluid keep in touch, for drawing the cleaning fluid in reaction cup; Hour hand, for injecting cleaning fluid in reaction cup.
In one embodiment, multiple described flexible pipe is polytetrafluoro flexible pipe.
In the utility model embodiment, each suction is washed pin to be connected with a membrane pump by a flexible pipe, adopt speed-regulating control circuit output waveform signals to trigger each membrane pump and regulate operating rate, make it possible to regulate operating rate for each membrane pump respectively, and then can to the cleaning fluid capture velocity in each reaction cup, therefore, while guaranteeing that lost package is not by magnetic bead, when running into fluid path water resistance and being uneven, can fully automatically regulate the cleaning fluid rate of liquid aspiration of single reaction cup, to reduce scavenging period.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with embodiment and accompanying drawing, the utility model is described in further details.At this, exemplary embodiment of the present utility model and illustrating for explaining the utility model, but not as to restriction of the present utility model.
In the utility model embodiment, provide a kind of chemical illumination immunity analysis instrument reaction cup cleaning fluid rate of liquid aspiration regulating device, this chemical illumination immunity analysis instrument reaction cup cleaning fluid rate of liquid aspiration regulating device can comprise: pin is washed in multiple suction, multiple flexible pipe, multiple membrane pump, speed-regulating control circuit, can regulate multiple reaction cup cleaning fluid capture velocity respectively, as shown in Figure 1, to be adjusted to example to describe speed Principles of Regulation to a reaction cup cleaning fluid capture velocity, each described suction is washed pin 8 and is contacted with the cleaning fluid 10 in a reaction cup 9, each described suction is washed pin 8 and is connected with a described membrane pump 11 by a described flexible pipe 12, multiple described membrane pump 11 is all connected with described speed-regulating control circuit 1, wherein,
Described speed-regulating control circuit 1, for output multi-channel waveform signal, triggers by every road waveform signal the described membrane pump be connected with described speed-regulating control circuit and regulates operating rate, wherein, and described Multichannel Waveform signal and described multiple membrane pump one_to_one corresponding;
Described membrane pump 11, washes pin for adopting the operating rate after adjustment by described suction and draws cleaning fluid in reaction cup.
Known as shown in Figure 1, in the utility model embodiment, each suction is washed pin to be connected with a membrane pump by a flexible pipe, adopt speed-regulating control circuit output waveform signals to trigger each membrane pump and regulate operating rate, make it possible to regulate operating rate for each membrane pump respectively, and then can to the cleaning fluid capture velocity in each reaction cup, such as, need 4 groups of suctions to wash pin at cleaning station and respectively absorption cleaning fluid is carried out to 4 reaction cup, during because of drawing liquid pipeline water resistance imbalance, there will be 4 reaction cup at the same time drawing liquid time, in fact the phenomenon that the drawing liquid speed of 4 reaction cup is inconsistent, now, adopt the chemical illumination immunity analysis instrument reaction cup cleaning fluid rate of liquid aspiration regulating device in the application, just can regulate operating rate for certain membrane pump, and then regulate separately the cleaning fluid rate of liquid aspiration of certain reaction cup.Therefore, while guaranteeing that lost package is not by magnetic bead, when running into fluid path water resistance and being uneven, can fully automatically regulate the cleaning fluid rate of liquid aspiration of single reaction cup, to reduce scavenging period.
Concrete, in prior art, peristaltic pump realizes drawing cleaning fluid action by roller extruding silica gel hose, silica gel hose is under long-time extruding, cause silica gel hose to be out of shape, the silica gel hose adopted between cleaning fluid and peristaltic pump after a long time use, occurs that material goes bad, affect cleaning performance, in the present embodiment, in order to avoid flexible pipe occurs that material goes bad, the problem of cleaning performance is affected, multiple described flexible pipe 12 is polytetrafluoro flexible pipes, this polytetrafluoro flexible pipe tolerance cleaning fluid.
During concrete enforcement, operating rate is regulated in order to each membrane pump can be adjusted subtly, in the present embodiment, above-mentioned speed-regulating control circuit 1, specifically for output multi-channel composite wave-shape signal, triggers by pulsed frequency and the change of pulse width of every road composite wave-shape signal the described membrane pump be connected with described speed-regulating control circuit and regulates operating rate.Such as, cleaning station need 4 groups inhale wash pin respectively absorption cleaning fluid is carried out to 4 reaction cup time, above-mentioned speed-regulating control circuit 1 can export 4 road composite wave-shape signals, and this 4 road composite wave-shape signal powder is applied to respectively 4 groups and inhales and wash on membrane pump that pin connects separately, to control the operating rate of 4 membrane pumps respectively, and then control the speed that 4 reaction cup draw cleaning fluid respectively, owing to being the operating rate being regulated membrane pump by composite wave-shape signal, carry out compared with membrane pump drives with employing PWM waveform, make the speed of membrane pump arrange scope can expand and be twice, speed regulation stall is meticulousr, can within the scope of 1ml/s-5ml/s the operating rate of accurate adjustment membrane pump, and then make membrane pump stably be operated in suitable velocity range, this scope is 2ml/s-5ml/s.
Concrete, described speed-regulating control circuit 1 becomes large specifically for the pulse width by modulating composite wave-shape signal and the pulsed frequency of modulation composite wave-shape signal diminishes, and triggers the described membrane pump be connected with described speed-regulating control circuit and reduces operating rate.
During concrete enforcement, above-mentioned speed-regulating control circuit 1 comprises: pulse width modulated waveform generator, for generation of multiplex pulse wide modulation waveform signal and multichannel low frequency square-wave signal (such as, as shown in Figure 2, multiplex pulse wide modulation waveform signal Zhong mono-tunnel pulse width modulated waveform signal can be PWM1, and multichannel low frequency square-wave signal Zhong mono-tunnel low frequency square-wave signal can be FRQ1), pulse modulated circuit, for by multiplex pulse wide modulation waveform signal Zhong mono-tunnel pulse width modulated waveform signal and multichannel low frequency square-wave signal Zhong mono-tunnel low frequency square-wave signal, be modulated to a road composite wave-shape signal (such as, DRV1 as shown in Figure 2), to form multichannel composite wave-shape signal, light lotus root buffer circuit, the input end of described smooth lotus root buffer circuit is connected with the output terminal of described pulse modulated circuit, the described smooth output terminal of lotus root buffer circuit is connected with the control end of membrane pump, for carrying out the isolation of light lotus root (wherein to multichannel composite wave-shape signal, light lotus root buffer circuit is by membrane pump operating circuit and the isolation of digital operation circuit, reduce membrane pump inductive load to the interference of control circuit, convert the composite wave-shape signal that pulse modulated circuit exports to voltage required for membrane pump work again), and the multichannel composite wave-shape signal after being isolated by light lotus root exports to membrane pump respectively.
During concrete enforcement, as shown in Figure 1, above-mentioned chemical illumination immunity analysis instrument reaction cup cleaning fluid rate of liquid aspiration regulating device also comprises: external circuit 2 (or host computer), for receiving the speed parameter of the operating rate of described membrane pump; Described speed-regulating control circuit, comprising: communicating circuit (such as, can be communication chip), for the speed parameter of the multiple described membrane pump be connected with described speed-regulating control circuit is sent to described external circuit.Such as, communicating circuit can also receive speed parameter instruction and the whether work order that cleaning fluids drawn by multiple membrane pumps that external circuit 2 sends, according to the speed parameter drawing cleaning fluid, generate the waveform parameter controlling pulse modulated circuit in above-mentioned speed-regulating control circuit, this waveform parameter is pulsed frequency and pulse width mainly
During concrete enforcement, in chemical illumination immunity analysis instrument, each reaction cup that antibody complex is housed will through 3-4 cleaning, cross pollution rate is an important index, corresponding bag is by the cleaning of magnetic bead, the maximum possible producing Residual contamination inhales to wash pin when declining the cleaning fluid drawn in reaction cup, do not mate because inhaling to wash pin decline rate and draw cleaning fluid speed, aspirating needle front end is caused to be immersed in cleaning fluid, unconjugated unnecessary antigen and enzymic-labelled antibody are after cleaning fluid sucks, be attached on the outer wall of aspirating needle, bag in next reaction cup is polluted by magnetic bead liquid, therefore for avoiding owing to cleaning the cross pollution caused, in the present embodiment, as shown in Figure 1, pin 8 is washed in described suction, comprise: minute hand 8a, the front end 8c of described minute hand 8a and the liquid level of cleaning fluid 10 keep in touch (such as, the speed drawing cleaning fluid is needed to regulate in 2ml/s-5ml/s, range estimation suction washes pin 8 in decline process, membrane pump is drawn the speed of cleaning fluid and is inhaled the speed cooperation of washing pin 8 and declining, the liquid level of the cleaning fluid 10 in the front end 8c of aspirating needle 8 and reaction cup 9 remains on contact condition, the cross pollution produced when effectively avoiding magnetic bead to clean), for drawing the cleaning fluid in reaction cup 9, hour hand 8b, for injecting cleaning fluid in reaction cup 9, namely injects not still for that clean, clean cleaning fluid in reaction cup 9.
In the utility model embodiment, each suction is washed pin to be connected with a membrane pump by a flexible pipe, adopt speed-regulating control circuit output waveform signals to trigger each membrane pump and regulate operating rate, make it possible to regulate operating rate for each membrane pump respectively, and then can to the cleaning fluid capture velocity in each reaction cup, therefore, while guaranteeing that lost package is not by magnetic bead, when running into fluid path water resistance and being uneven, can fully automatically regulate the cleaning fluid rate of liquid aspiration of single reaction cup, to reduce scavenging period.
The foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, for a person skilled in the art, the utility model embodiment can have various modifications and variations.All within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.