CN110873660A - Magnetic separation device, magnetic separation method and sample analysis device - Google Patents
Magnetic separation device, magnetic separation method and sample analysis device Download PDFInfo
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- CN110873660A CN110873660A CN201811013403.4A CN201811013403A CN110873660A CN 110873660 A CN110873660 A CN 110873660A CN 201811013403 A CN201811013403 A CN 201811013403A CN 110873660 A CN110873660 A CN 110873660A
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- 238000007885 magnetic separation Methods 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000004458 analytical method Methods 0.000 title abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 135
- 239000011324 bead Substances 0.000 claims abstract description 66
- 239000002699 waste material Substances 0.000 claims abstract description 28
- 238000007599 discharging Methods 0.000 claims abstract description 14
- 238000003018 immunoassay Methods 0.000 claims abstract description 11
- 230000009471 action Effects 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims description 56
- 238000002347 injection Methods 0.000 claims description 32
- 239000007924 injection Substances 0.000 claims description 32
- 238000002955 isolation Methods 0.000 claims description 32
- 239000000243 solution Substances 0.000 claims description 20
- 238000000605 extraction Methods 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 description 9
- 238000000926 separation method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 238000009826 distribution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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Abstract
The invention discloses a magnetic separation device, a magnetic separation method and a sample analysis device, wherein the device comprises: a magnetic separation unit, comprising: the magnetic separation disc is provided with at least one cup placing position for accommodating and placing a reaction container, wherein the reaction container contains magnetic beads and solution for immunoassay test; the at least one magnet is correspondingly arranged in the at least one cup placing position respectively, a corresponding annular magnet is arranged in each cup placing position, so that when the reaction container is arranged in the cup placing positions, the magnetic beads are adsorbed on the inner wall of the reaction container under the action of the magnetic force of the annular magnet in the cup placing positions, and the magnetic beads are prevented from being discharged when waste liquid in the reaction container is discharged. Through the mode, the magnetic bead discharging device can avoid waste caused by the fact that the magnetic beads are discharged, and meanwhile, a pipeline is prevented from being blocked by the magnetic beads.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a magnetic separation device, a magnetic separation method and a sample analysis device.
Background
The immunomagnetic bead separation technology is a new separation technology combining the high specificity of immunology and the specific magnetic responsiveness of magnetic beads, is an immunology detection method and an antigen purification means with strong specificity and high sensitivity, and is widely applied to the aspects of cell separation, protein detection, immunology detection, microbiological detection and the like.
In order to separate the non-target substance in a free state from the target substance combined with the magnetic beads, the reactant needs to be magnetically separated and washed in a magnetic field, the washing process is completed by repeatedly circulating the steps of extracting supernatant waste liquid in the reaction cup and then re-injecting the washing liquid in the magnetic field, and the loss rate of the target magnetic beads in the washing process is closely related to the effect of magnetic separation. And for the pressure of drawing the supernatant pump, under the circumstances of rated magnetic field intensity, it is too big to draw the supernatant pump negative pressure, can lead to adhering to the magnetic bead on the reaction cup inner wall to be siphoned away, and this just makes to have a large amount of immunomagnetic beads in the liquid that the drainage needle discharges, has not only caused the waste of immunomagnetic beads, still can block up corresponding pipeline and lead to equipment trouble.
In the long-term research and development process, the inventor of the application finds that the loss rate of magnetic beads is high and the pipeline is easy to be blocked in the conventional magnetic separation device.
Disclosure of Invention
The invention mainly solves the technical problem of providing a secondary separation device, a magnetic separation method and a sample analysis device, which can avoid waste caused by the discharge of magnetic beads in the magnetic separation process and avoid the blockage of a pipeline by the magnetic beads.
In order to solve the technical problems, the invention adopts a technical scheme that: a magnetic separation device is provided.
Wherein the apparatus comprises:
a magnetic separation unit, comprising:
the magnetic separation disc is provided with at least one cup placing position for accommodating and placing a reaction container, wherein the reaction container contains magnetic beads and solution for immunoassay test;
at least one annular magnet which is respectively and correspondingly arranged in the at least one cup placing position, wherein the corresponding annular magnet is arranged in each cup placing position, so that when the reaction container is arranged in the cup placing positions, the magnetic beads are adsorbed on the inner wall of the reaction container by utilizing the magnetic force of the annular magnet in the cup placing positions, and the magnetic beads are prevented from being discharged when waste liquid in the reaction container is discharged.
In order to solve the technical problem, the invention adopts another technical scheme that: a magnetic separation method is provided.
Wherein the method comprises the following steps:
placing a reaction container containing magnetic beads for immunoassay test and a solution in a cup placing position in a magnetic separation disc, and rotating the magnetic separation disc to transfer the reaction container to the position below a liquid discharge needle, wherein a corresponding annular magnet is arranged in the cup placing position in the magnetic separation disc, so that the free magnetic beads in the reaction container are adsorbed to the inner wall of the reaction container under the action of the annular magnet;
and moving the liquid discharge needle downwards to a proper position in the reaction vessel, and discharging the solution in the reaction vessel by using the liquid discharge needle.
In order to solve the technical problem, the invention adopts another technical scheme that: a sample analyzer is provided.
Wherein the sample analyzer comprises any one of the magnetic separation device and the sample detection device.
The invention has the beneficial effects that: different from the prior art, the annular magnet is arranged at the cup placing position, so that the magnetic beads are adsorbed on the inner wall of the reaction container under the magnetic force action of the annular magnet when the reaction container is placed at the cup placing position, and the magnetic separation of the magnetic beads is realized. And annular magnet can to reaction vessel applys more stable and the bigger magnetic field of magnetic field intensity, can adsorb more magnetic beads reaction vessel's inner wall, improves the separation efficiency of magnetic bead, and then avoid the waste that the magnetic bead was discharged and is caused, also avoid the pipeline quilt the magnetic bead blocks up.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic structural view of one embodiment of a magnetic separation apparatus of the present invention;
FIG. 2 is a cross-sectional view of the magnetic separation device of FIG. 1;
FIG. 3 is a schematic structural diagram of one embodiment of a liquid discharge unit of the present invention;
FIG. 4 is a schematic structural view of an embodiment of the cleaning unit of the present invention;
FIG. 5 is a schematic flow diagram of one embodiment of a magnetic separation method of the present invention;
FIG. 6 is a schematic flow chart diagram of one embodiment of a magnetic separation method of the present invention;
FIG. 7 is a schematic structural diagram of an embodiment of a sample analyzer according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 and 2, fig. 1 is a schematic structural view of an embodiment of a magnetic separation apparatus according to the present invention, and fig. 2 is a cross-sectional view of the magnetic separation apparatus of fig. 1, the apparatus including:
a magnetic separation unit, comprising: a magnetic separation disk 10 having at least one cup placing position 20 for accommodating a reaction container 30, wherein the reaction container contains magnetic beads for performing an immunoassay test and a solution; at least one ring magnet 40 respectively and correspondingly disposed in the at least one cup placing position 20, wherein each cup placing position 20 is disposed with the corresponding ring magnet 40, so that when the reaction vessel 30 is disposed in the cup placing position 20, the magnetic beads are adsorbed on the inner wall of the reaction vessel 30 by the magnetic force of the ring magnet 40 in the cup placing position 20, thereby preventing the magnetic beads from being discharged when the waste liquid in the reaction vessel 30 is discharged.
In the present embodiment, the ring-shaped magnet is disposed at the cup placing position, so that when the reaction vessel 30 is placed at the cup placing position 20, the magnetic beads are adsorbed on the inner wall of the reaction vessel 30 by the magnetic force of the ring-shaped magnet 40, thereby realizing the magnetic separation of the magnetic beads. And ring magnet 40 can to reaction vessel 30 applys more stable and the bigger magnetic field of magnetic field intensity, can adsorb more magnetic beads at reaction vessel 30's inner wall, improves the separation efficiency of magnetic bead, and then avoids the magnetic bead is discharged the waste that causes, also avoids the pipeline quilt the magnetic bead blocks up.
In the present embodiment, the ring magnet 40 is wrapped around the cup placing position 20, and the ring magnet 40 may be a permanent magnet or an electromagnet. In one embodiment, when the ring magnet 40 is a permanent magnet, the ring magnet 40 has different thicknesses along the extension direction of the cup placing position to match the number distribution gradient of the magnetic beads in different regions of the reaction vessel 30 for further cost reduction. Furthermore, in order to accurately control the magnetic field intensity, the ring magnet 40 is an electromagnet, and the electromagnet is connected with a control circuit. Furthermore, the electro-magnet is a plurality of edges put the annular magnet unit that the extending direction of cup position distributes, and every the annular magnet unit is connected with solitary control circuit, so that the field intensity distribution in the magnetic field that annular magnet produced with the number distribution gradient of different regional magnetic beads corresponds among reaction vessel 30, realizes right the high-efficient separation of magnetic bead avoids the too big magnetic bead that leads to on reaction cup inner wall of adhesion to be siphoned away simultaneously and causes extravagantly and also avoids the magnetic bead to get into the pipeline and block up.
In another embodiment, referring to fig. 3, fig. 3 is a schematic structural diagram of a liquid discharge unit according to an embodiment of the present invention, and the liquid discharge device 100 is used for discharging waste liquid in a reaction container 30, for example, supernatant in a reaction cup used in a magnetic separation and washing process of a reactant in an immunomagnetic bead separation technology. The liquid discharge device 100 comprises a liquid discharge needle 110, an extraction pump 120 and an isolation chamber 130, wherein the liquid discharge needle 110 is used for extracting waste liquid in the reaction container; the extraction pump 120 is connected with the drainage needle 110 through a drainage pipeline so as to provide extraction power for the drainage needle 110; the isolation chamber 130 is disposed on a drain line between the drain needle 110 and the draw pump 120 to cooperate with the draw pump 120 to buffer and regulate the drawing power supplied to the drain needle.
In the present embodiment, the isolation chamber 130 provided in the liquid discharge line between the liquid discharge needle 110 and the extraction pump 120 can adjust the extraction power supplied to the liquid discharge needle 110 in cooperation with the extraction pump 120, and this buffer adjustment method is simple, can further reduce the loss rate of magnetic beads, and facilitates maintenance of the liquid path device. Also adopt the magnetic separation unit that is equipped with ring magnet to mutually support with the flowing back unit that is equipped with the isolation chamber, reducing and leaving over the magnetic bead in reaction vessel's the waste liquid figure in, rationally adjust extraction power, can obtain lower magnetic bead loss rate, further reduce the risk of pipeline jam, the corresponding device's of being convenient for maintenance obtains better use and experiences.
In the present embodiment, the drainage device 100 may be an independent liquid path device, or may be a liquid path unit in any equipment, for example, a liquid path unit of a cleaning device in an immunoassay analyzer, which may be used in combination with other liquid path units in the cleaning device, for example, a cleaning unit for injecting a cleaning solution, to complete the whole cleaning process.
Specifically, the drainage needle 110 of the drainage device 100 is used to be inserted into a reaction vessel to extract waste liquid in the reaction vessel, such as supernatant in a reaction cup. The suction pump 120 is connected to the drainage needle 110 through a drainage line 140 to provide suction power, such as negative pressure, to the drainage needle 110. And the isolation chamber 130 is provided on the drain line 140 between the drain needle 110 and the draw pump 120 to cooperate with the draw pump 120 to buffer-regulate the drawing power supplied to the drain needle 110.
In this embodiment, the extraction pump 120 may be a diaphragm pump, which does not require frequent replacement of the associated tubing, and which has a longer life and facilitates better access for maintenance than a peristaltic pump. The specific structural design of the drainage pipe 140 may be determined according to practical applications, and is not limited herein. The isolation chamber 130 and the diaphragm pump 120 are disposed on the drain line 140, and the isolation chamber 130 is connected to the diaphragm pump 120, so that the isolation chamber 130 can play a role of buffering the negative pressure pumping power when the diaphragm pump 120 adjusts the pumping power of the negative pressure. The diaphragm pump 120 may also be referred to as a control pump, which receives a control signal from a controller or computer during a control process to change the flow rate of the fluid and maintain a tuning parameter (e.g., duty cycle parameter) within a desired range, thereby automating the manufacturing process.
In addition, the drainage device 100 may further include a first solenoid valve 150, such as a two-position three-way solenoid valve, in which a closed chamber is provided, through holes are opened at different positions, each hole connects different passages, a piston is in the middle of the chamber, two electromagnets are provided at both sides, and which magnet coil is energized to which side the valve body is attracted, so that it can open or close different through holes by controlling the movement of the valve body. That is, the two-position three-way electromagnetic valve is controlled by double coils, one coil is powered on instantly, then the power supply is closed, the valve is opened, and the corresponding channels are communicated with each other; the other coil is instantaneously energized and then the power supply is turned off, the valve is closed, and the corresponding passages are communicated with each other. The two-position three-way electromagnetic valve can be kept in a closed or opened state for a long time, so that the service life of the coil is longer, and the two-position three-way electromagnetic valve is best used in a high-temperature pipeline.
In the present embodiment, as shown in fig. 3, the isolation chamber 130 is provided with a first opening 131, a second opening 132 and a third opening 133, wherein the first opening 131 is provided at the upper end of the sidewall of the isolation chamber 130, the second opening 132 is provided at the lower end of the sidewall of the isolation chamber 130, and the third opening 133 is provided at the top end of the isolation chamber 130. The normally open end 150A of the first solenoid valve 150 is connected to the first opening 131 of the isolation chamber 130 through the drain line 140, the normally closed end 150B of the first solenoid valve 150 is connected to the second opening 132 of the isolation chamber 130 through the drain line 140, and the working end 150C of the first solenoid valve 150 is connected to the draw pump 120 through the drain line 140; and the third opening 133 of the isolation chamber 130 is connected to the drainage needle 110 through the drainage line 140.
In the present embodiment, the diaphragm pump 120 can cooperate with the first solenoid valve 150 to adjust the negative pressure suction force input into the liquid discharge device 100, and when the first solenoid valve 150 is switched to the normally open end 150A and opened, the diaphragm pump 120 is connected to the first opening 131 of the isolation chamber 130 to provide the suction force for the normal operation of the liquid discharge needle 110 to draw the supernatant in the reaction cup; when the first solenoid valve 150 is switched to the normally closed end 150B and opened, the diaphragm pump 120 is connected to the second opening 132 of the isolation chamber 130, and the waste liquid accumulated in the isolation chamber 130 can be extracted. In the whole liquid discharging process, the isolation chamber 130 can buffer and regulate the negative pressure extraction power input into the liquid discharging device 100, so that the extraction force in the liquid discharging needle 110 is not too strong, and the target magnetic beads in the reaction cup are extracted while the liquid discharging needle 110 extracts the supernatant in the reaction cup.
As shown in fig. 3, the drainage device 100 further includes a waste liquid tank 160, and the waste liquid tank 160 is disposed at the end of the drainage pipe 140 and connected to the diaphragm pump 120 through the drainage pipe 140. Therefore, the waste liquid pumped and discharged by the drainage device 100 can be injected into the waste liquid barrel 160 through the relevant passage.
In another embodiment, the magnetic separation apparatus further comprises a cleaning unit 200 for injecting a cleaning solution into the reaction vessel 40 placed on the cup placing position 20, and referring to fig. 4, fig. 4 is a schematic structural diagram of an embodiment of the cleaning unit of the present invention, and the cleaning unit 200 is used for providing a new cleaning solution for the reaction vessel 30. That is, after the waste liquid (supernatant) in the reaction container 30 is discharged by the liquid discharge unit 100 shown in fig. 3, a new cleaning liquid is further injected into the reaction container 30 by the cleaning unit 200 to clean the target objects (e.g., target magnetic beads) in the reaction container again, and the waste liquid is discharged by the liquid discharge unit 210 after the cleaning, so that the target objects can be cleaned for a plurality of times.
Specifically, the cleaning unit 200 includes a cleaning liquid supplier 221, a liquid injection needle 222, and a plunger pump 223. Wherein, the cleaning solution supplier 221 is used for accommodating and supplying the cleaning solution; the liquid injection needle 222 is connected to the cleaning liquid supplier 221 through a liquid injection line 225 to obtain the cleaning liquid from the cleaning liquid supplier 221 and supply the cleaning liquid to the reaction vessel; the plunger pump 223 is disposed on the liquid injection line 225 and connected to the cleaning liquid supplier 221 and the liquid injection needle 222 to provide a power source to deliver the cleaning liquid in the cleaning liquid supplier 221 to the liquid injection needle 222.
Further, the washing unit 220 may further include a second solenoid valve 224, for example, a two-position three-way solenoid valve. Wherein the working end of the second electromagnetic valve 224 is connected to the plunger pump 223, the normally open end of the second electromagnetic valve 224 is connected to the liquid injection line 225, and the normally closed end of the second electromagnetic valve 224 is connected to the cleaning liquid supply 221. Therefore, the second electromagnetic valve 224 cooperates with the plunger pump 223 to deliver the cleaning liquid in the cleaning liquid supplier 221 to the liquid injection needle 222 by the plunger pump 223 according to the demand.
Furthermore, as shown in fig. 3 and 4, in the present embodiment, the drainage needle 110 in the drainage unit 100 may include a long needle 1111 and a short needle 1112 arranged side by side, wherein the long needle 1111 is connected to the isolation chamber 130 and the extraction pump 120 through the drainage pipe 216 to extract the waste liquid, i.e., to perform the drainage function. And short needle 1112 is disposed adjacent to long needle 1111 and is connected to fluid injection line 225 in cleaning unit 200 to draw cleaning fluid from cleaning unit 220 to clean the outer wall of long needle 2111.
Specifically, in the present embodiment, the cleaning unit 200 may further include a third solenoid valve 226 provided in the liquid injection line 225 between the second solenoid valve 224 and the liquid injection needle 222. The third solenoid valve 226 may be a two-position three-way solenoid valve, the working end of the third solenoid valve 226 is connected to the liquid injection line 225, the normally open end of the third solenoid valve 226 is connected to the liquid injection needle 222, and the normally closed end of the third solenoid valve 226 is connected to the short needle 2112. That is, it is possible to control whether the cleaning liquid is injected into the injection needle 222 by the third solenoid valve 226 to add the cleaning liquid to the cuvette or to control the injection of the cleaning liquid into the short needle 1112 to clean the outer wall of the long needle 1111.
In another embodiment, referring to fig. 1 and fig. 3 together, the magnetic separation unit further includes a driving device 50, and the driving mechanism 50 is connected to the magnetic separation disk 10 to drive the magnetic separation disk 10 to rotate so as to rotate the reaction vessel 30 placed on the cup placement position 20 to below the liquid discharge needle 110 or to below the liquid injection needle 222. In this embodiment, the driving mechanism 50 can rotate the reaction container 30 on the cup placing position 20 to a position below the liquid discharge needle 110 or a position below the liquid injection needle 222, i.e., the liquid discharge and cleaning process can be completed without repeatedly moving the reaction container 30 directly to wash away impurities on the surface of the magnetic beads.
Further, the magnetic separation unit further comprises a manipulator (not shown) for clamping the reaction vessels 30, which have been cleaned and separated from the magnetic beads, from the magnetic separation disc 10 to the area to be detected for subsequent detection. In this embodiment, the subsequent assay may be an immunoassay assay. Namely, the reaction vessel 30, which has been cleaned and separated from the magnetic beads, is transferred to the lower part of the sample needle by the manipulator, and then the operation of mixing, sucking and discharging and the operation of immunoassay are performed.
In order to solve the technical problem, the invention adopts another technical scheme that: a magnetic separation method is provided.
Referring to fig. 5, fig. 5 is a schematic flow chart of an embodiment of a magnetic separation method according to the present invention, wherein the method includes the steps of:
s100, placing a reaction container containing magnetic beads for immunoassay test and a solution in a cup placing position in a magnetic separation disc, and rotating the magnetic separation disc to transfer the reaction container to the lower part of a liquid drainage needle.
In the step S100, a corresponding ring magnet is disposed in the cup placing position in the magnetic separation disc, so that the free magnetic beads in the reaction container are adsorbed to the inner wall of the reaction container under the action of the ring magnet.
Specifically, the reaction vessel to be magnetically separated is grasped to a cup placing position on the magnetic separation disc by the manipulator, the reaction vessel on the cup placing position is transposed to the lower part of the liquid discharge needle (i.e., rotated to a double needle position), and the reaction apparatus is stood in a magnetic field.
S200, moving the liquid discharge needle downwards to a proper position in the reaction container, and discharging the solution in the reaction container by using the liquid discharge needle.
In the step S200, the drainage needle is driven by the linear motor to move in a direction approaching the reaction vessel, enter the reaction vessel, and move to a proper position. And then, the diaphragm pump is opened, the duty ratio parameter of the diaphragm pump is properly adjusted, and the supernatant waste liquid is discharged to a waste liquid barrel through the long needle of the liquid discharge needle under the action of the negative-pressure pumping power so as to discharge the solution in the reaction container. Further, the first solenoid valve is switched to the normally closed end, and the isolation pump is fully operated to evacuate the residual liquid in the isolation chamber.
Further, referring to fig. 6, fig. 6 is a schematic flow chart of another embodiment of a magnetic separation method according to the present invention, wherein the method further includes the steps of:
s300, removing the liquid discharging needle, and rotating the magnetic separation disc to enable the reaction container to rotate to the position below the liquid injection needle, so that cleaning liquid is injected into the reaction container through the liquid injection needle.
In the step S300, the liquid discharging needle is removed, the magnetic separation disc rotates, the reaction device rotates to a position below the liquid injection needle (i.e., to a single needle position), the plunger pump is turned on, and the cleaning liquid is injected into the reaction cup through the liquid injection needle. Furthermore, the stirring mechanism is inserted into the proper position of the reaction device for stirring, so that the impurities on the surfaces of the magnetic beads can be quickly cleaned, and the cleaning times are reduced.
S400, rotating the magnetic separation disc again to enable the reaction container to rotate to the position below the liquid discharge needle, and repeating the steps to complete the magnetic separation cleaning operation for a preset number of times.
In the step S400, the magnetic separation disk is rotated again to rotate the reaction vessel to a position below the liquid discharge needle, and the above steps are repeated to complete the magnetic separation cleaning operation for a predetermined number of times. The number of the magnetic separation washing operations may be determined according to the number of the magnetic beads in the reaction device and the amount of the washing solution injected each time, and is not particularly limited herein.
In order to solve the technical problem, the invention adopts another technical scheme that: a sample analyzer is provided. Referring to fig. 7, fig. 7 is a schematic structural diagram of a sample analyzer according to an embodiment of the present invention, wherein the sample analyzer 1 includes any one of the magnetic separation device 10 and the sample detection device 20. The detection device 20 may be an immunofluorescence detection device.
In summary, the present invention discloses a magnetic separation apparatus and method, the apparatus includes: a magnetic separation unit, comprising: the magnetic separation disc is provided with at least one cup placing position for accommodating and placing a reaction container, wherein the reaction container contains magnetic beads and solution for immunoassay test; the at least one magnet is correspondingly arranged in the at least one cup placing position respectively, a corresponding annular magnet is arranged in each cup placing position, so that when the reaction container is arranged in the cup placing positions, the magnetic beads are adsorbed on the inner wall of the reaction container under the action of the magnetic force of the annular magnet in the cup placing positions, and the magnetic beads are prevented from being discharged when waste liquid in the reaction container is discharged. Through the mode, the magnetic bead discharging device can avoid waste caused by the fact that the magnetic beads are discharged, and meanwhile, a pipeline is prevented from being blocked by the magnetic beads.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (14)
1. A magnetic separation device, comprising: a magnetic separation unit, comprising:
the magnetic separation disc is provided with at least one cup placing position for accommodating and placing a reaction container, wherein the reaction container contains magnetic beads and solution for immunoassay test;
at least one annular magnet which is respectively and correspondingly arranged in the at least one cup placing position, wherein the corresponding annular magnet is arranged in each cup placing position, so that when the reaction container is arranged in the cup placing positions, the magnetic beads are adsorbed on the inner wall of the reaction container by utilizing the magnetic force of the annular magnet in the cup placing positions, and the magnetic beads are prevented from being discharged when waste liquid in the reaction container is discharged.
2. A magnetic separating device according to claim 1 wherein the ring magnet is a permanent magnet having a thickness gradient in the direction of extent of the cupped location.
3. A magnetic separation device according to claim 1 wherein the ring magnet is an electromagnet, the magnetic separation device further comprising a control circuit electrically connected to the electromagnet, the electromagnet comprising a plurality of ring magnet units distributed along the extension of the cup holding position, and each ring magnet unit being connected to a separate control circuit unit.
4. A magnetic separation device according to claim 1 further comprising: a liquid discharge unit comprising:
the liquid discharge needle is used for extracting waste liquid in the reaction container;
the extraction pump is connected with the liquid discharge needle through a liquid discharge pipeline so as to provide extraction power for the liquid discharge needle;
and the isolation chamber is arranged on a liquid discharge pipeline between the liquid discharge needle and the extraction pump and is matched with the extraction pump to buffer and adjust the extraction power provided for the liquid discharge needle.
5. A magnetic separation device according to claim 4 further comprising:
a cleaning unit for injecting a cleaning solution into the reaction vessel placed on the cup placing position, comprising:
a cleaning liquid supplier for receiving and supplying a cleaning liquid;
the liquid injection needle is connected with the cleaning liquid supplier through a liquid injection pipeline so as to obtain the cleaning liquid from the cleaning liquid supplier and provide the cleaning liquid to the reaction container;
and the plunger pump is arranged on the liquid injection pipeline and is connected with the cleaning liquid supplier and the liquid injection needle so as to provide a power source for conveying the cleaning liquid in the cleaning liquid supplier to the liquid injection needle.
6. A magnetic separation device according to claim 5 wherein the magnetic separation unit further comprises:
and the driving device is connected with the magnetic separation disc to drive the magnetic separation disc to rotate so as to enable the reaction container placed on the cup placing position to rotate below the liquid discharging needle or rotate below the liquid injecting needle.
7. A magnetic separation device according to claim 1 wherein the magnetic separation unit further comprises:
and the manipulator is used for clamping the reaction container which is cleaned and separated by the magnetic beads from the magnetic separation disc to the area to be detected so as to perform subsequent detection.
8. A magnetic separation device according to claim 5 wherein the drainage needle comprises a long needle and a short needle arranged side by side, wherein the long needle is connected to the isolation chamber and the extraction pump by a drainage line to extract waste liquid; and the short needle is connected with the liquid injection pipeline to obtain cleaning liquid to clean the long needle.
9. A magnetic separation device according to claim 4 wherein the drainage unit further comprises:
a first solenoid valve disposed on a drain line between the isolation chamber and the diaphragm pump, wherein the first solenoid valve is a two-position three-way solenoid valve, and the isolation chamber is provided with a first opening, a second opening, and a third opening, wherein the first opening is disposed at an upper end of a sidewall of the isolation chamber, the second opening is disposed at a lower end of the sidewall of the isolation chamber, and the third opening is disposed at a top end of the isolation chamber, a normally open end of the first solenoid valve is connected to the first opening of the isolation chamber through a drain line, a normally closed end of the first solenoid valve is connected to the second opening of the isolation chamber through a drain line, and a working end of the first solenoid valve is connected to the extraction pump through a drain line; and the third opening of the isolation chamber is connected to the drainage needle through a drainage line.
10. A magnetic separation device according to claim 5 wherein the wash unit further comprises:
and the working end of the second electromagnetic valve is connected with the plunger pump, the normally open end of the second electromagnetic valve is connected with the liquid injection pipeline, and the normally closed end of the second electromagnetic valve is connected with the cleaning liquid supplier.
11. A magnetic separation device according to claim 5 wherein the wash unit further comprises:
and the working end of the third electromagnetic valve is connected with the liquid injection pipeline, the normally open end of the third electromagnetic valve is connected with the liquid injection needle, and the normally closed end of the third electromagnetic valve is connected with the short needle.
12. A magnetic separation method, comprising:
placing a reaction container containing magnetic beads for immunoassay test and a solution in a cup placing position in a magnetic separation disc, and rotating the magnetic separation disc to transfer the reaction container to the position below a liquid discharge needle, wherein a corresponding annular magnet is arranged in the cup placing position in the magnetic separation disc, so that the free magnetic beads in the reaction container are adsorbed to the inner wall of the reaction container under the action of the annular magnet;
and moving the liquid discharge needle downwards to a proper position in the reaction vessel, and discharging the solution in the reaction vessel by using the liquid discharge needle.
13. A method of magnetic separation according to claim 12 further comprising:
removing the liquid discharge needle, and rotating the magnetic separation disc to enable the reaction container to rotate to be below the liquid injection needle so as to inject cleaning liquid into the reaction container through the liquid injection needle;
and rotating the magnetic separation disc again to enable the reaction container to rotate to the position below the liquid discharge needle, and repeating the steps to complete the magnetic separation and cleaning operation for a preset number of times.
14. A sample analyzer, comprising the magnetic separation device according to any one of claims 1 to 11 and a sample detection device.
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| CN201811013403.4A CN110873660A (en) | 2018-08-31 | 2018-08-31 | Magnetic separation device, magnetic separation method and sample analysis device |
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| CN201811013403.4A CN110873660A (en) | 2018-08-31 | 2018-08-31 | Magnetic separation device, magnetic separation method and sample analysis device |
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