CN110721123A

CN110721123A - Anti-aging skin care composition

Info

Publication number: CN110721123A
Application number: CN201911089922.3A
Authority: CN
Inventors: 陈娟
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2020-01-24

Abstract

The invention discloses an anti-aging skin care composition, which mainly comprises Spanish moss (TILLANDSIA USNEOIDES) extract and arginine ferulate. Experiments prove that the anti-aging composition has a good inhibition effect on non-enzymatic glycosylation reaction, and can enhance skin elasticity, obviously improve fine wrinkles and play a good anti-aging role when applied to cosmetics.

Description

Anti-aging skin care composition

Technical Field

The invention relates to the field of cosmetics, and particularly relates to an anti-aging skin care composition.

Background

Aging is a process of progressive functional deterioration occurring at the cellular and body level, and physiological aging is irreversible degeneration of complex morphological structure and physiological function of the whole body gradually along with the increase of age, influenced by genetic factors, from the beginning of the maturation stage of an organism. Pathological aging is the accelerated aging caused by disease or abnormal factors. The aging of the population is challenged in most countries of the world, aging and aging-related diseases impair the quality of life of people, and the aging delaying, especially the reduction of skin surface aging-related symptoms (such as wrinkles, banning, dryness, etc.), has become a major target for the exploration of the cosmetic industry.

The theory of non-enzymatic glycosylation aging is the focus of the research on the anti-aging mechanism of cosmetics in recent years. The non-enzymatic glycosylation reaction in vivo refers to the process of generating reversible or irreversible conjugate, Advanced glycation end-products (AGEs), by reacting aldehyde group or ketone group of reducing sugar with free amino group in macromolecules such as protein under the condition of no enzyme catalysis. Dyer et al found that skin aging is related to non-enzymatic glycosylation, the dermis layer of the skin is rich in collagen and elastin, amino acids in protein molecules are easy to perform non-enzymatic glycosylation with aldehyde groups or ketone groups of glucose in extracellular fluid, AGEs are increased progressively with the increase of age, collagen forms intermolecular cross-linking, the elasticity and permeability of the skin are reduced, the diffusion performance of nutrients and wastes is weakened, the solubility of collagen is reduced, collagen is difficult to hydrolyze by collagenase, the elasticity of the skin is reduced, wrinkles are difficult to smooth and deepen continuously, and the aging process of the skin is accelerated.

Based on the above, the invention provides an anti-aging skin care composition, and experiments prove that the anti-aging composition has a good inhibition effect on non-enzymatic glycosylation reaction, and can enhance skin elasticity, obviously improve fine lines and play a good anti-aging role when being applied to cosmetics, thereby completing the invention.

Disclosure of Invention

The invention aims to provide an anti-aging skin care composition.

The specific technical scheme for solving the technical problems is as follows:

an anti-aging skin care composition comprises the following components in percentage by mass: spanish moss (Tillandsiausneoides) extract 0.5-5%, arginine ferulate 0.1-1%.

In one embodiment, the anti-aging skin care composition comprises the following components in percentage by mass: 3% of Spanish moss (TILLANDSIA USNEOIDES) extract and 0.5% of arginine ferulate.

The extracts of Spanish moss (TILLANDSIA USNEOIDES) described herein represent extracts derived from Spanish moss which may contain one or more bioactive molecules including terpenoids and phenolic compounds.

Suitable extracts of Spanish moss (TILLANDSIA USNEOIDES) may be commercially available products or may be prepared by the following process: (1) cleaning aerial parts of fresh Spanish moss, drying in the shade, pulverizing, adding ethanol water solution, stirring and soaking at room temperature to obtain soaking solution; (2) filtering the soaking solution, and distilling under reduced pressure on a rotary evaporator to remove ethanol in the solution to obtain extract.

In some embodiments, the ethanol aqueous solution added in the step (1) is 2-4 times of Spanish moss in weight, and the ethanol aqueous solution has a volume concentration of 60-70% (V/V).

In some embodiments, the soaking time in step (2) is 12-24 hours, the filtration mesh number is 100-500 meshes, and the temperature of the reduced pressure distillation is 30-40 ℃.

In some embodiments, the anti-aging skin care composition further comprises the following components in percentage by mass: fullerene 0.0001-0.01%, and radix Pulsatillae (Pulsatilla CHINENSIS) extract 1-5%.

In some embodiments, the anti-aging skin care composition further comprises the following components in percentage by mass: radix Paeoniae (Paeonia alba) root extract 1%, and fructus Zanthoxyli (Zanthoxylum BUNGEANUM) fruit extract 1%.

The technical scheme provided by the embodiment of the invention has the following beneficial effects:

(1) according to the invention, Spanish moss (TILLANDSIA USNEOIDES) extract and arginine ferulate are selected as matching materials, so that the Spanish moss has a good inhibition effect on non-enzymatic glycosylation reaction, and can be applied to cosmetics to enhance skin elasticity, obviously improve fine wrinkles and play a good anti-aging effect;

(2) the preparation process of the Spanish moss (TILLANDSIA USNEOIDES) extract provided by the invention is carried out at low temperature, so that the effective components are retained to the maximum extent; and the required equipment is simple and convenient, and the large-scale production is easy to realize.

Drawings

FIG. 1 shows the inhibition of non-enzymatic glycosylation by a test sample;

FIG. 2 skin elasticity test results;

figure 32 canthus skin before and 8 weeks after product use by subjects.

Detailed Description

The embodiments in the description are only for illustrating the present invention and do not limit the scope of the present invention. The scope of the present invention is defined only by the appended claims, and any omissions, substitutions, or modifications made based on the embodiments disclosed herein will fall within the scope of the present invention.

For the purpose of enhancing understanding of the present invention, the present invention will be further described in detail with reference to the following examples, which are provided for illustration only and are not to be construed as limiting the scope of the present invention.

The extract of pulsatillae radix (PULSATILLA CHINENSIS) of the present invention is a standard extract of the aerial parts of pulsatillae radix plant. The extract of Pulsatillae radix (Pulsatilla CHINENSIS) can be prepared by extracting by common process, such as soaking and extracting with water for 1-3 times, mixing extractive solutions, and filtering.

The peony (Paeonia ALBIFLORA) root extract is a standard extract of peony roots. The radix Paeoniae (Paeonia ALBIFLORA) root extract can be prepared by common extraction process, such as extracting with 65% (V/V) ethanol water solution for 1-3 times, mixing extractive solutions, filtering, and rotary evaporating to remove ethanol.

The ZANTHOXYLUM BUNGEANUM (ZANTHOXYLUM BUNGEANUM) fruit extract is a standard extract of ZANTHOXYLUM BUNGEANUM fruits. The fructus Zanthoxyli (Zanthoxylum BUNGEANUM) extract can be prepared by extracting with water as solvent for 1-3 times, mixing extractive solutions, and filtering.

Example 1

A Spanish moss (TILLANDSIA USNEOIDES) extract is prepared by extracting Spanish moss by the following process: (1) cleaning aerial parts of fresh Spanish moss, drying in the shade, pulverizing, adding 60% (V/V) ethanol water solution 4 times of Spanish moss, stirring and soaking at room temperature for 24 hr to obtain soaking solution; (2) filtering the soaking solution with 100 mesh filter cloth, and vacuum distilling at 40 deg.C in rotary evaporator to remove ethanol to obtain extract.

Example 2

A Spanish moss (TILLANDSIA USNEOIDES) extract is prepared by extracting Spanish moss by the following process: (1) cleaning aerial parts of fresh Spanish moss, drying in the shade, pulverizing, adding 70% (V/V) ethanol water solution 2 times of Spanish moss, stirring and soaking at room temperature for 12 hr to obtain soaking solution; (2) filtering the soaking solution with 500 mesh filter cloth, and vacuum distilling at 30 deg.C in rotary evaporator to remove ethanol to obtain extract.

Example 3 inhibition assay for non-enzymatic glycosylation reactions

(1) Preparation of sample to be tested

The samples to be tested were prepared according to the proportions in Table 1.

TABLE 1 compositions of samples to be tested

Wherein Spanish moss (TILLANDSIA USNEOIDES) extract was prepared as in example 2.

(2) Construction of non-enzymatic glycosylation System

Referring to a research method (Liu Jie, Zhang Wei hong, and the like; efficacy research of sweet osmanthus extract on the aspect of skin aging resistance [ J ]. spice essence cosmetics, 2019(02): 23-26.), bovine serum albumin and fructose are mixed and incubated to simulate a non-enzymatic glycosylation reaction in a human body, an inhibitor of the non-enzymatic glycosylation reaction is added to inhibit the reaction, the fluorescence intensity of an inhibitor system is weaker than that of a control without the inhibitor, and the inhibition rate is used for reflecting the inhibition of the sample on the non-enzymatic glycosylation reaction.

(3) Test procedure

Taking a certain amount of mixed solution containing 10 mg/mL bovine serum albumin and 0.25 mol/L fructose as reaction liquid, adding a sample solution to be detected with the mass fraction of 1.00%, setting a negative control group (PBS replaces the sample), a positive control group (Vc ethyl ether with the mass fraction of 1.00%) and a blank group (PBS replaces the reaction liquid), and setting 3 parallel tubes in each group. Incubate at 37 ℃ in the dark. The Fluorescence intensity (RFU, Relative Fluorescence Unit) of each test group was measured at 370 nm (absorption wavelength)/440 nm (excitation wavelength) using a Fluorescence microplate reader selected at 1, 2, 3, 5 and 7 d, and the inhibition ratio was calculated according to the formula.

Inhibition rate = [ 1- (RFU)_{Inhibiting group}-RFU_{Blank group}）/RFU_{Negative control group}】×100%

The results of the test for inhibiting non-enzymatic glycosylation for each sample to be tested are shown in FIG. 1. As can be seen from FIG. 1, the extract of Spanish moss (TILLANDSIA USNEOIDES) and arginine ferulate, when used together, have a good inhibitory effect on non-enzymatic glycosylation reactions.

Example 4

The skin-refreshing water essence with the anti-aging effect comprises the following components in percentage by mass:

1, 3-propylene glycol 5%, glycerol 5%, betaine 1%, bioglycan gum-10.5%, saccharide isomerate 0.5%, xanthan gum 0.1%, arginine ferulate 0.1%, Spanish moss (TILLANDSIA USNEOIDES) extract 5%, squalane 0.1%, lecithin 0.5%, sodium acetylated hyaluronate 0.05%, panthenol 0.5%, Sclerotinia sclerotiorum (Sclerotium ROLFSSII) gum 0.1%, sodium polyacrylate 0.1%, sodium hyaluronate 0.05%, sodium polyglutamate 0.05%, caprylyl hydroximic acid 0.3%, hexylene glycol 0.5%, citric acid 0.05%, and water in balance.

Example 5

1, 3-propylene glycol 5%, glycerol 5%, betaine 1%, bioglycan gum-10.5%, saccharide isomerate 0.5%, xanthan gum 0.1%, arginine ferulate 1%, Spanish moss (TILLANDSIA USNEOIDES) extract 0.5%, squalane 0.1%, lecithin 0.5%, sodium hyaluronate 0.05%, panthenol 0.5%, Sclerotinia sclerotiorum (Sclerotium ROLFSSII) gum 0.1%, sodium polyacrylate 0.1%, sodium hyaluronate 0.05%, sodium polyglutamate 0.05%, caprylyl hydroximic acid 0.3%, hexylene glycol 0.5%, citric acid 0.05%, and water in balance.

Example 6

1, 3-propylene glycol 5%, glycerol 5%, betaine 1%, bioglycan-10.5%, saccharide isomerate 0.5%, xanthan gum 0.1%, arginine ferulate 0.5%, Spanish moss (TILLANDSIA USNEOIDES) extract 3%, squalane 0.1%, lecithin 0.5%, acetylated sodium hyaluronate 0.05%, panthenol 0.5%, Sclerotinia sclerotiorum (Sclerotium ROLFSSII) gum 0.1%, sodium polyacrylate 0.1%, sodium hyaluronate 0.05%, sodium polyglutamate 0.05%, octanoyl hydroximic acid 0.3%, hexylene glycol 0.5%, citric acid 0.05%, fullerene 0.0001%, Pulsatilla CHINENSIS (Pulsatilla CHINENSIS) extract 5%, and the balance water.

Example 7

1, 3-propylene glycol 5%, glycerol 5%, betaine 1%, bioglycan-10.5%, saccharide isomerate 0.5%, xanthan gum 0.1%, arginine ferulate 0.5%, Spanish moss (TILLANDSIA USNEOIDES) extract 3%, squalane 0.1%, lecithin 0.5%, acetylated sodium hyaluronate 0.05%, panthenol 0.5%, Sclerotinia sclerotiorum (Sclerotium ROLFSSII) gum 0.1%, sodium polyacrylate 0.1%, sodium hyaluronate 0.05%, sodium polyglutamate 0.05%, octanoyl hydroximic acid 0.3%, hexylene glycol 0.5%, citric acid 0.05%, fullerene 0.01%, Pulsatilla CHINENSIS (Pulsatilla CHINENSIS) extract 1%, and balance water.

Example 8

1, 3-propylene glycol 5%, glycerol 5%, betaine 1%, bioglycan-10.5%, saccharide isomerate 0.5%, xanthan gum 0.1%, arginine ferulate 0.5%, Spanish moss (TILLANDSIA USNEOIDES) extract 3%, squalane 0.1%, lecithin 0.5%, acetylated sodium hyaluronate 0.05%, panthenol 0.5%, Sclerotinia (SCLEOTIUM ROLFSSII) gum 0.1%, sodium polyacrylate 0.1%, sodium hyaluronate 0.05%, sodium polyglutamate 0.05%, octanoyl hydroximic acid 0.3%, hexylene glycol 0.5%, citric acid 0.05%, fullerene 0.01%, Pulsatilla CHINENSIS (PULSATILLA CHINENSIS) extract 1%, Paeonia lactiflora (PAEONIAALBIFLORA) root extract 1%, Zanthoxylum BUNGEANUM fruit extract 1%, and water in balance.

Example 9

The rejuvenating water extracts prepared in examples 4-8 were evaluated for volunteer efficacy and a blank set was set, wherein the blank set was distinguished from example 4 by the absence of arginine ferulate and spanish moss (TILLANDSIA USNEOIDES) extract.

The specific test method is as follows:

(1) volunteer summons

60 healthy women aged 35 ~ 60 were divided into product and blank groups, 30 persons/group.

Inclusion criteria were: the complexion of the face is dull, the eye corners have obvious fine lines, and the skin lacks elasticity; the test can be well matched, and the life rule can be kept during the research period; all the contents of the informed consent can be read and understood and signed voluntarily; during the test period, the skin care products were discontinued, no further clinical trials were involved in any other research center, and no use of any cosmetics, drugs and nutraceuticals affecting the outcome was agreed to. Exclusion criteria: the skin disease at the tested part may affect the judgment of the test result; those with high allergic constitution; a female who is pregnant, lactating, or intended to be pregnant during the test; severe center of gravity, liver and kidney function damage and severe immunologic hypofunction; those with mental disorders, severe endocrine disorders, and oral contraceptives; adding drugs into clinical testers or other testers of the drugs within 30 days, or patients who have systemic effect on the test result within nearly 1 week; those who had oral and topical cosmetic products within 2 weeks that may have an effect on the outcome of the test; the test person can not be matched; researchers considered unsuitable participants.

(2) Test arrangement

After cleaning the skin, a proper amount of sample is taken and wiped on the facial skin, once in the morning and at night. Before use, after 4 and 8 weeks of use, subjects washed their faces and rested for 15 min in an environment with a temperature of 21 ± 1 ℃ and a relative humidity of 50% ± 5% RH, the subjects were evaluated by a dermatologist, skin elasticity, gloss, wrinkles were detected, and the subjects completed self-evaluation.

(3) Test method

The skin elasticity tester Cutomer MPA580 detects elasticity index R2 of cheek skin, the average value is taken after 3 times of measurement, the R2 value is the ratio of the rebound quantity of the skin without negative pressure to the maximum stretching quantity with negative pressure, and the R2 value is closer to 1, which shows that the skin elasticity is better. The specific test results are shown in fig. 2.

2 tested volunteers were selected, and the wrinkle condition of the volunteers' eyes was photographed by using a facial image analyzer VISIA, and the specific test results are shown in fig. 3.

As can be seen from fig. 2, after the product is used for 4 and 8 weeks, the skin elasticity of the volunteers is obviously increased, and as can be seen from fig. 3, the wrinkles of the eyes of the volunteers are improved, so that the rejuvenating water essence provided by the embodiment of the invention has a good anti-aging effect.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The anti-aging skin care composition is characterized by comprising the following components in percentage by mass: 0.5-5% of Spanish moss (TILLANDSIA USNEOIDES) extract and 0.1-1% of arginine ferulate.

2. The anti-aging skin care composition according to claim 1, which comprises the following components in percentage by mass: 3% of Spanish moss (TILLANDSIA USNEOIDES) extract and 0.5% of arginine ferulate.

3. The anti-aging skin care composition according to claim 1 or 2, wherein the Spanish moss (TILLANDSIA USNEOIDES) extract is prepared by the following process: (1) cleaning aerial parts of fresh Spanish moss, drying in the shade, pulverizing, adding ethanol water solution, stirring and soaking at room temperature to obtain soaking solution; (2) filtering the soaking solution, and distilling under reduced pressure on a rotary evaporator to remove ethanol in the solution to obtain extract.

4. The anti-aging skin care composition according to claim 3, wherein the ethanol aqueous solution in step (1) is added in an amount of 2 to 4 times the weight of Spanish moss, and the concentration of the ethanol aqueous solution by volume is 60 to 70% (V/V).

5. The anti-aging skin care composition according to claim 3, wherein the soaking time in step (2) is 12-24 hours, the filtration mesh number is 100-500 meshes, and the temperature of the reduced pressure distillation is 30-40 ℃.

6. The anti-aging skin care composition according to claim 1 or 2, further comprising the following components in percentage by mass: fullerene 0.0001-0.01%, and radix Pulsatillae (Pulsatilla chinensis) extract 1-5%.

7. The anti-aging skin care composition according to claim 1 or 2, further comprising the following components in percentage by mass: radix Paeoniae (Paeonia alba) root extract 1%, and fructus Zanthoxyli (Zanthoxylum BUNGEANUM) fruit extract 1%.