CN110694008A

CN110694008A - Dongshan longevity-prolonging and senescence-delaying composition and preparation method thereof

Info

Publication number: CN110694008A
Application number: CN201910999814.3A
Authority: CN
Inventors: 苏军峰
Original assignee: GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF MEDICATION
Current assignee: GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF MEDICATION
Priority date: 2019-10-21
Filing date: 2019-10-21
Publication date: 2020-01-17

Abstract

The invention discloses a composition for prolonging life and delaying senescence in east China and a preparation method thereof, wherein the composition comprises the following raw materials in parts by weight: 106 portions of gynostemma pentaphylla, 107 portions of prepared fleece flower root, 113 portions of prepared fleece flower root, 58 to 62 portions of fructus cannabis, 107 portions of rhizoma polygonati, 113 portions of rhizoma gastrodiae, 28 to 32 portions of rhizoma gastrodiae, 58 to 62 portions of polygonatum, 28 to 32 portions of ganoderma lucidum, 48 to 52 portions of eucommia bark, 19 to 21 portions of liquorice, 106 portions of euonymus fortunei, 114 portions of tinospora, 108 portions of paniculate swallowwort root, 112 portions of crataegus leaves and 108 portions of large-fruit hawthorn leaves. The invention also discloses a processing technology of the composition, and the obtained composition has the effects of tonifying qi and blood, nourishing liver and kidney, calming heart and invigorating spleen, and can be used for dizziness, palpitation, insomnia, senile weakness, weakness after illness, iron deficiency anemia, cerebral arteriosclerosis, hyperlipidemia, coronary heart disease, chronic gastritis, chronic colitis, neurasthenia, climacteric syndrome and athletic syndrome. Can be used for adjuvant treatment of cancer and pulmonary tuberculosis. Can eliminate fatigue, delay senility and strengthen body's disease resistance after long-term use.

Description

Dongshan longevity-prolonging and senescence-delaying composition and preparation method thereof

Technical Field

The invention relates to the technical field of medicines, in particular to a composition for prolonging life and delaying senescence in Dongshan and a preparation method thereof.

Background

According to traditional Chinese medicine, a human body is an organic whole, the human body takes the five internal organs as the center, the five internal organs, the five sense organs, the nine parts and the four limbs and bones are mutually connected through the meridian action, essence, qi and blood body fluids form basic substances for maintaining the life activities of the human body, and the basic substances are necessary energy for the physiological activities of the human body. However, its generation and metabolism depend on normal physiological activities of organs such as zang-fu organs and meridians. If the deficiency of physiological organs and the deficiency of qi, blood, yin and yang can cause various chronic debilitation, the traditional Chinese medicine summarizes the chronic debilitation as follows: category of consumptive disease.

The causes of consumptive disease are many, however, essence qi seizing causes the theory of deficiency and excess, the theory of essence qi commenting on deficiency and excess causes the outline of deficiency syndrome, and the theory of fatigue and fatigue includes fatigue, fatigue and weakness of people who do not consider it, deficiency and weakness after illness, fatigue of people who do not consider it, strain of people who do not consider it, congenital, acquired, pockmarked rash, illness and exopathic causes, and medical causes. 'Lianxueyuan-deficiency syndrome with six reasons' summarizing the diseases of viscera qi-blood yin-yang deficiency caused by deficiency of innate essence, malnutrition in the afterdays, weak constitution due to long-term illness, internal injury due to overstrain and chronic insufficiency as main manifestations, all belong to consumptive diseases. Differentiation of syndromes based on the principle of qi, blood, yin and yang, with false five zang organs as the target, the book Su Wen & san Jiu Bian Lun (treatise on Su Wen & san Ji Jiu Bian): "tonifying the deficiency syndrome. So the treatment is basically tonifying.

The syndrome of both qi deficiency and blood deficiency is manifested as dizziness, shortness of breath, disinterest to speak, lung and spleen qi storing, weakness and spontaneous perspiration, pale or sallow complexion, palpitation and insomnia, heart-throb and insomnia, blood deficiency failing to fill veins, pale tongue and tender face, inability to nourish muscles to cause emaciation, thready and weak pulse, and deficiency of both qi and blood due to chronic illness, failure of vital energy to produce blood, or failure of blood to transform qi; liver stores blood and kidney stores essence, which are bred and transformed mutually, so that the liver and kidney are homologous, liver and kidney deficiency is the syndrome of liver blood deficiency and kidney essence deficiency, which is specifically represented by vertigo, tinnitus, white and lusterless face, insufficient blood for stabilizing spirit, night sleep, dreaminess and kidney essence deficiency, alopecia, odontoseisis, foot flaccidity, weakness and weakness, brain marrow, essence deficiency, marrow deficiency, amnesia, absentmindedness, early senility of adults, pale tongue with white fur, deep and thready pulse, deficiency of spleen and kidney, insufficient sources of biochemical origin, insufficient endowment and acquired recuperation loss, and liver blood is injured by chronic diseases to cause liver and kidney deficiency; the heart-spleen deficiency syndrome is the syndrome of heart-blood deficiency and spleen-qi deficiency, the heart governs blood, spleen governs blood, and spleen is the source of qi and blood generation, so the relationship between heart and spleen is close, the spleen functions normally, and the function of blood generation and transformation is vigorous. Blood is abundant, so heart governs. The spleen-qi deficiency and dysfunction of transportation and transformation are manifested as palpitation, severe palpitation, insomnia, dreaminess, vertigo, amnesia, sallow complexion, inappetence, abdominal distension, loose stool, lassitude, hypodynamia or chronic bleeding, pale and tender tongue, thready and weak pulse, heart-spleen deficiency caused by long-term disorder, fatigue, weakness or chronic bleeding.

Nowadays, with the aggravation of life pressure and irregular diet of people, work, study and daily life are seriously affected, and therefore, the five internal organs must be nourished by making the human body strong and full of essence, qi, blood and body fluid.

Disclosure of Invention

The invention aims to solve the problems and provides a Dongshan longevity-prolonging senescence-delaying composition and a preparation method thereof.

In order to achieve the purpose of the invention, the invention adopts the technical scheme that:

the composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 106 portions of gynostemma pentaphylla, 107 portions of prepared fleece flower root, 113 portions of prepared fleece flower root, 58 to 62 portions of fructus cannabis, 107 portions of rhizoma polygonati, 113 portions of rhizoma gastrodiae, 28 to 32 portions of rhizoma gastrodiae, 58 to 62 portions of polygonatum, 28 to 32 portions of ganoderma lucidum, 48 to 52 portions of eucommia bark, 19 to 21 portions of liquorice, 106 portions of euonymus fortunei, 114 portions of tinospora, 108 portions of paniculate swallowwort root, 112 portions of crataegus leaves and 108 portions of large-fruit hawthorn leaves.

Further, the composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of ganoderma lucidum, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortune, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root and 110 parts of big fruit hawthorn leaf.

Further comprises 58-62 parts of longan aril and 110-115 parts of shiitake mushroom.

The invention provides a preparation method of a composition for prolonging life and delaying senescence in east China, which comprises the following steps:

(1) respectively slicing Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes, freezing at-18 deg.C for 5-8 hr, respectively pulverizing, and performing ultrasonic wall breaking treatment at 12-15 deg.C to obtain wall-broken Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes dry powder;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi into coarse powder, soaking in 65-75 vol% ethanol solution for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 15-20 vol% of percolate to obtain caulis Seu folium Euonymi Fortunei and fructus crataegi extract;

(3) crushing the tinospora cordifolia and the paniculate swallowwort root, putting the crushed tinospora cordifolia and the paniculate swallowwort root into an enzymolysis tank, adding cellulase and pectinase, carrying out enzymolysis for 1-2 hours at the temperature of 35-40 ℃ under the negative pressure condition, and carrying out spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding 25-30 mL of ethanol solution with the volume concentration of 65-75% to soak for 2-3 h, concentrating and recovering ethanol, and freeze-drying to obtain an enzymolysis extract;

(4) decocting the rest medicinal materials with water twice, wherein the first time is to add 3 times of water by mass of the medicinal materials for 2 hours, the second time is to add 2 times of water by mass of the medicinal materials for 1.5 hours, the two filtrates are combined, the filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a first extract, ethanol with the mass of 3 times of the filtrate is added into the first extract, the mixture is stirred uniformly and then stands for 24 hours, then the filtration is carried out, the filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a second extract, the second extract is diluted with water and stirred uniformly, the supernatant is taken out for filtration, and the obtained filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a mixture extract.

Further, the dry gastrodia tuber powder, the wintercreeper hawthorn extract, the enzymolysis extract and the mixture extract are combined to prepare oral liquid, granules, capsules or tablets.

And (3) further comprising the step (5) after the step (4), wherein the step (5) is to add 16-20 parts by weight of sucrose syrup and 0.06-0.08 part by weight of sodium benzoate into the prepared ganoderma lucidum, gastrodia elata, dried longan pulp and lentinus edodes powder, wintercreeper and hawthorn extract, the enzymolysis extract and the mixture extract, add water to the total weight of the oral liquid of 1000 parts, boil, filter, fill and sterilize to obtain the oral liquid of the composition for prolonging life and delaying senility.

Further, in the step (3), the dosage of the cellulase is 0.2-0.3% of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.1-0.2% of the total weight of the tinospora root and the paniculate swallowwort root.

Further, in the step (1), the ganoderma lucidum is pulverized by putting the ganoderma lucidum into a nanometer ultrafine pulverizer to be pulverized to a particle size of 300-500 meshes.

Further, in the step (1), the operation time of the ultrasonic wall breaking is 15-30 minutes.

The composition has the following raw materials in beneficial effects and compatibility principle:

1. in the formula, the five medicines are combined to be a monarch medicine together; the four medicines are used as a Chinese and ministerial medicine in the prescription to strengthen the effect of a monarch medicine; the pseudo-ginseng has the functions of nourishing blood and activating blood, and the fructus cannabis has the functions of nourishing yin, moistening intestines and activating blood, and are used as adjuvant drugs together; licorice root, radix Glycyrrhizae coordinates as a guiding drug. The medicines are combined to play the roles of tonifying qi and enriching blood, nourishing liver and kidney, calming heart and invigorating spleen. Can be used for treating dizziness, palpitation, insomnia, asthenia, iron deficiency anemia, cerebral arteriosclerosis, hyperlipidemia, coronary heart disease, chronic gastritis, chronic colitis, neurasthenia, climacteric syndrome, and athletic syndrome. Can be used for adjuvant treatment of cancer and pulmonary tuberculosis. Can eliminate fatigue, delay senility and strengthen body's disease resistance after long-term use. In addition, the formula is skillfully added with fructus cannabis for moistening the viscera and relaxing the bowels, so the formula is tonifying without dryness, nourishing without stagnation, and the formula has strict structures of monarch, minister, assistant and guide medicines, and has the effects of strengthening the body resistance and eliminating the pathogenic factors, and the threads are screwed into the buttons.

2. In addition, the added arillus longan and lentinus edodes in the formula can further enhance the drug effect, wherein the arillus longan is used for tonifying heart and spleen, nourishing blood and soothing nerves; the lentinus edodes has the effects of nourishing blood and tonifying qi, stimulating appetite and helping eating, resisting tumor, delaying senility and the like; can improve the efficacy of invigorating qi and blood and building up body when added into the formula.

3. Meanwhile, modern pharmacology proves that: rhizoma Polygonati can enhance immunity and prolong T cell life; the lucid ganoderma and the pseudo-ginseng expand coronary artery, improve blood circulation of the coronary artery and improve the hypoxia tolerance; the lucid ganoderma has the functions of increasing leucocytes and protecting the liver; caulis Seu folium Euonymi Fortunei has effects of relaxing muscles and tendons, activating collateral flow, stopping bleeding, and removing blood stasis; the radix tinosporae has the effects of clearing away heat and toxic materials, relieving sore throat, and relieving swelling and pain; the native ginseng famous sphaerocephala herb is artemisia maderocephala, has the efficacy of clearing heat and removing toxicity, and has certain curative effects on qi deficiency and hypodynamia, spleen deficiency and diarrhea, lung dryness and cough, neurasthenia and the like; the flavone in the hawthorn leaves of the large fruits has the function of improving the activity of hippocampal acetylcholine transferase and is helpful for the memory strength; the fleece-flower root is known to have the effect of prolonging life from old times, and pharmacological experiments prove that the fleece-flower root has an anti-aging component lecithin and can also promote the regeneration and development of red blood cells. Gynostemma pentaphylla is originally a daily beverage of longevity people which can prolong life by being handed down early in folk in east orchid, Bama areas of Guangxi, is locally commonly called 'longevity tea', and when the professor of Japan bamboo Ben Chang Song announces that the Gynostemma pentaphylla contains more than 50 similar ginsenosides in the discussion of Canada ginseng at the end of 1979, the Gynostemma pentaphylla has the efficacy of strengthening body resistance and eliminating evil.

4. The invention provides a corresponding preparation method, effective components in the medicine can be efficiently extracted by the method, and the content of active component 2,3,5, 4' -tetrahydroxystilbene-2-0-beta-D glucoside in the prepared composition is more than or equal to 0.252 mg/ml.

Detailed Description

The present invention will be described in further detail with reference to the following examples, but the present invention is not limited thereto.

Example 1

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of lucid ganoderma, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortunei, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root, 110 parts of big fruit hawthorn leaf, 58 parts of arillus longan and 110 parts of mushroom;

the preparation method of the Dongshan longevity-prolonging senescence-delaying composition specifically comprises the following steps:

(1) slicing Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes, freezing at-18 deg.C for 5 hr, pulverizing respectively, breaking cell wall of rhizoma Gastrodiae, arillus longan and Lentinus Edodes at 12 deg.C for 15 min, pulverizing Ganoderma in nanometer micronizer to 300 mesh to obtain cell wall-broken Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes dry powder;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi Pinnatifidae into coarse powder, soaking in 65% ethanol solution for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 15% of percolate volume to obtain caulis Seu folium Euonymi Fortunei fructus crataegi extract;

(3) pulverizing radix tinosporae and radix ginseng, placing into an enzymolysis tank, adding cellulase and pectinase, performing enzymolysis at 35 deg.C under negative pressure for 1 hr, and spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding ethanol solution with volume concentration of 65% into 25mL, soaking for 2h, concentrating, recovering ethanol, and freeze drying to obtain enzymolysis extract; the dosage of the cellulase is 0.2 percent of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.1 percent of the total weight of the tinospora root and the paniculate swallowwort root;

(4) decocting the rest medicinal materials with water twice, wherein the first time is to add 3 times of water by mass of the medicinal materials for 2 hours, the second time is to add 2 times of water by mass of the medicinal materials for 1.5 hours, the two filtrates are combined, the filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a first extract, ethanol with the mass of 3 times of the filtrate is added into the first extract, the mixture is stirred uniformly and then stands for 24 hours, then the filtration is carried out, the filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a second extract, the second extract is diluted with water and stirred uniformly, the supernatant is taken out for filtration, the obtained filtrate is concentrated in vacuum to the relative density of 1.16-1.20 at 60 ℃ to obtain a mixture extract;

(5) adding 16 parts by weight of sucrose syrup and 0.06 part by weight of sodium benzoate into the prepared ganoderma lucidum, gastrodia elata, arillus longan and lentinus edodes dry powder, euonymus fortunei and hawthorn extract, enzymolysis extract and mixture extract, adding water to the total weight of the oral liquid to 1000 parts by weight, boiling, filtering, filling and sterilizing to obtain the oral liquid of the composition for prolonging life and delaying senility.

Example 2

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of lucid ganoderma, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortunei, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root, 110 parts of big fruit hawthorn leaves, 62 parts of arillus longan and 115 parts of mushroom;

(1) slicing Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes, freezing at-18 deg.C for 8 hr, pulverizing respectively, breaking cell wall of rhizoma Gastrodiae, arillus longan and Lentinus Edodes at 15 deg.C for 30 min, pulverizing Ganoderma in nanometer micronizer to particle size of 500 mesh to obtain cell-broken dry powder of Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi Pinnatifidae into coarse powder, adding 75% ethanol solution, soaking for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 20% of percolate volume to obtain caulis Seu folium Euonymi Fortunei fructus crataegi extract;

(3) pulverizing radix tinosporae and radix ginseng, placing into an enzymolysis tank, adding cellulase and pectinase, performing enzymolysis at 40 deg.C under negative pressure for 2 hr, and spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding 30mL of ethanol solution with volume concentration of 75% to soak for 3h, concentrating and recovering ethanol, and freeze-drying to obtain an enzymolysis extract; the dosage of the cellulase is 0.3 percent of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.2 percent of the total weight of the tinospora root and the paniculate swallowwort root;

(5) the oral liquid is prepared by adding 20 parts by weight of sucrose syrup and 0.08 part by weight of sodium benzoate into the prepared ganoderma lucidum, gastrodia elata, arillus longan and lentinus edodes dry powder, wintercreeper and hawthorn extract, enzymolysis extract and mixture extract, adding water to the total weight of the oral liquid to 1000 parts by weight, boiling, filtering, filling and sterilizing to obtain the Dongshan oral liquid of the composition for prolonging life and delaying senility.

Example 3

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of lucid ganoderma, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortunei, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root, 110 parts of big fruit hawthorn leaf, 60 parts of arillus longan and 113 parts of mushroom;

(1) slicing Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes, freezing at-18 deg.C for 7 hr, pulverizing respectively, breaking cell wall of rhizoma Gastrodiae, arillus longan and Lentinus Edodes at 14 deg.C for 16 min, pulverizing Ganoderma in nanometer micronizer to 400 mesh to obtain cell wall-broken Ganoderma, rhizoma Gastrodiae, arillus longan and Lentinus Edodes dry powder;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi Pinnatifidae into coarse powder, adding 70% ethanol solution, soaking for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 16% of percolate volume to obtain caulis Seu folium Euonymi Fortunei fructus crataegi extract;

(3) pulverizing radix tinosporae and radix ginseng, placing into an enzymolysis tank, adding cellulase and pectinase, performing enzymolysis at 37 deg.C under negative pressure for 1.5h, and spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding ethanol solution with volume concentration of 70% into 27mL, soaking for 2.5h, concentrating, recovering ethanol, and freeze drying to obtain enzymolysis extract; the dosage of the cellulase is 0.25 percent of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.15 percent of the total weight of the tinospora root and the paniculate swallowwort root;

(5) the oral liquid is prepared by adding 18 parts by weight of sucrose syrup and 0.07 part by weight of sodium benzoate into the prepared ganoderma lucidum, gastrodia elata, arillus longan and lentinus edodes dry powder, wintercreeper and hawthorn extract, the enzymolysis extract and the mixture extract, adding water to the total weight of the oral liquid to 1000 parts, boiling, filtering, filling and sterilizing to obtain the Dongshan oral liquid of the composition for prolonging life and delaying senility.

Example 4

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of ganoderma lucidum, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortune, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root and 110 parts of big fruit hawthorn leaf;

(1) slicing Ganoderma and rhizoma Gastrodiae, freezing at-18 deg.C for 6 hr, pulverizing, breaking cell wall of rhizoma Gastrodiae, arillus longan and Lentinus Edodes at 14 deg.C for 25 min, and pulverizing Ganoderma into 450 mesh powder;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi Pinnatifidae into coarse powder, adding 68% ethanol solution, soaking for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 16% of percolate volume to obtain caulis Seu folium Euonymi Fortunei fructus crataegi extract;

(3) pulverizing radix tinosporae and paniculate Bolbostemma rhizome, placing into an enzymolysis tank, adding cellulase and pectinase, performing enzymolysis at 36 deg.C under negative pressure for 1.2 hr, and spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding ethanol solution with volume concentration of 68% into 26mL, soaking for 2.2h, concentrating, recovering ethanol, and freeze drying to obtain enzymolysis extract; the dosage of the cellulase is 0.24 percent of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.13 percent of the total weight of the tinospora root and the paniculate swallowwort root;

(5) the oral liquid is prepared by adding 19 parts by weight of sucrose syrup and 0.065 part by weight of sodium benzoate into the prepared dry powder of the lucid ganoderma and the gastrodia elata, the wintercreeper hawthorn extract, the enzymolysis extract and the mixture extract, adding water to the total weight of the oral liquid to 1000 parts by weight, boiling, filtering, filling and sterilizing to obtain the Dongshan oral liquid of the composition for prolonging life and delaying aging.

Example 5

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 106 parts of gynostemma pentaphylla, 107 parts of prepared fleece flower root, 58 parts of fructus cannabis, 107 parts of rhizoma polygonati, 28 parts of rhizoma gastrodiae, 58 parts of radix polygonati officinalis, 28 parts of ganoderma lucidum, 48 parts of eucommia ulmoides, 19 parts of liquorice, 106 parts of euonymus fortunei, 106 parts of radix tinosporae, 108 parts of paniculate swallowwort root and 108 parts of big fruit hawthorn leaf;

(1) slicing Ganoderma and rhizoma Gastrodiae, freezing at-18 deg.C for 6 hr, pulverizing, breaking cell wall of rhizoma Gastrodiae, arillus longan and Lentinus Edodes at 13 deg.C for 25 min, and pulverizing Ganoderma into 350 mesh powder;

(2) pulverizing caulis Seu folium Euonymi Fortunei and folium crataegi Pinnatifidae into coarse powder, adding 72 vol% ethanol solution, soaking for 24 hr, percolating, recovering ethanol from percolate, and concentrating to 18 vol% of percolate to obtain caulis Seu folium Euonymi Fortunei fructus crataegi extract;

(3) pulverizing radix tinosporae and radix ginseng, placing into an enzymolysis tank, adding cellulase and pectinase, performing enzymolysis at 38 deg.C under negative pressure for 1.5h, and spray drying to obtain Chinese medicinal powder; mixing the traditional Chinese medicine powder according to the weight ratio of 1 g: adding ethanol solution with volume concentration of 72% into 28mL, soaking for 2.5h, concentrating, recovering ethanol, and freeze drying to obtain enzymolysis extract; the dosage of the cellulase is 0.27 percent of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.17 percent of the total weight of the tinospora root and the paniculate swallowwort root;

(5) the preparation of the oral liquid is that 18 parts of sucrose syrup and 0.075 part of sodium benzoate are added into the prepared ganoderma lucidum and gastrodia elata dry powder, wintercreeper hawthorn extract, enzymolysis extract and mixture extract, water is added to reach the total weight of 1000 parts of the oral liquid, and the oral liquid of the composition for prolonging life and delaying aging is obtained by boiling, filtering, filling and sterilizing.

Example 6

The composition for prolonging life and delaying aging of Dongshan comprises the following raw materials in parts by weight: 114 parts of gynostemma pentaphylla, 113 parts of prepared fleece-flower root, 62 parts of fructus cannabis, 113 parts of rhizoma polygonati, 32 parts of rhizoma gastrodiae, 62 parts of polygonatum odoratum, 32 parts of ganoderma lucidum, 52 parts of eucommia ulmoides, 21 parts of liquorice, 114 parts of euonymus fortune, 114 parts of tinospora cordifolia, 112 parts of panicled fameflower root and 1112 parts of large-fruit hawthorn leaf;

1 test on the immunomodulating Effect of the pharmaceutical composition (oral liquid prepared in example 1)

Test animals: mice weighing 18-21g were randomly divided into 4 groups of 10 mice each.

Dose grouping: 200mg/kg, 400mg/kg, 1200mg/kg and a negative control group, and after the stomach was drenched for 30 days, the following indexes were measured.

1.1 delayed type allergy (DTH) (ear swelling method)

Measurement and data processing: each mouse was sensitized by uniformly applying 50. mu.l of 1% DNFB solution to the abdomen hair cut with scissors in a range of about 3 cm. times.3 cm. After 5 days, the mice were challenged with 10. mu.l of 1% DNFB solution evenly applied to the right ears (both sides) while the mice were challenged with 10. mu.l of DNFB-free solution evenly applied to the left ears (both sides). Mice were sacrificed 24h after the challenge by cervical dislocation, the left and right concha were cut off, the 8mm diameter ear was removed with a punch and weighed. The difference between the weights of the left and right ears was used to represent the degree of DTH, and data statistics were performed using analysis of variance.

TABLE 1 Effect on DNFB Induction of mouse DTH

Group of	Dosage (mg/kg bw)	Animal number (only)	Ear shell weight gain (mg)
				Control group	0	10	8.2±2
Low dose group	200	10	8.8±1
				Middle dose group	400	10	9.3±3
High dose group	1200	10	11.7±1

Note: ". compared to control, P < 0.01.

The results in table 1 show that DTH of the high-dose group of the traditional Chinese medicine composition is significantly higher than that of the control group (P < 0.01).

1.2 serum hemolysin assay

Measurement and data processing: injecting 2% SRBC suspension 0.2ml into abdominal cavity of each mouse, removing eyeball and collecting blood after 4 days, and separatingAnd (5) separating serum. After 200-fold dilution of serum, the optical density values of the sample tube and the SRBC at half hemolysis were determined according to the test method. The amount of hemolysin is expressed as half the hemolysin value (HC)₅₀) Table 2 shows data statistics using analysis of variance.

TABLE 2 Effect on the formation of mouse serum hemolysin

Group of	Dosage (mg/kg bw)	Animal number (only)	HC₅₀
				Control group	0	10	78±51
Low dose group	200	10	123±66
				Middle dose group	400	10	153±62*
High dose group	1200	10	174±46**

Note: "+ compared to control, P < 0.05; ". compared to control, P < 0.01.

As shown in the results in Table 2, the serum hemolysin values of the high and medium dose groups are obviously higher than those of the control group (P <0.05 or P < 0.01).

1.3 carbon Cleaner test

Measurement and data processing: 3 times diluted India ink (0.1ml/10g body weight) was injected into the vein of rat tail, 20 μ l of blood was collected from angular venous plexus for 2min and 10min, respectively, and added to 2ml of 0.1% Na₂C0₃In the solution, the optical density value was measured with a spectrophotometer at a wavelength of 600 nm. At the same time, the mice were sacrificed and the livers, spleens, and thymus were weighed. Mice were characterized by corrected phagocytosis index a and differences in organ to body weight ratios between groups were compared by anova.

TABLE 3 Effect on carbon clearance in mice

Note: ". compared to control, P < 0.01.

The results in table 3 show that the phagocytosis index of the high and medium dose groups is higher than that of the control group (P <0.01), which indicates that the pharmaceutical composition provided by the invention can improve the carbon clearance capacity of mice.

2 test of antiaging Effect of the pharmaceutical composition (oral liquid prepared in example 1)

Test animals: SD female rats, 20 months old, were randomly divided into four groups of 10 animals each.

Dose grouping: 200mg/kg, 400mg/kg, 1200mg/kg · bw, and a control group, and the stomach was gavaged orally for 30 consecutive days.

The test steps are as follows: after 30 continuous days, the rat heart blood is taken and respectively measured for SOD, MDA and GSH-Px; the animals were then sacrificed and the liver was homogenized and the liver tissue was assayed for lipofuscin.

Data processing: data were analyzed for variance using SPSS10.0 for windows software (LSD method). The results of the data are as follows:

2.1 weight effects

TABLE 4 Effect on rat body weight

Group of	Animal number (only)	Initial body weight (g)	Middle-term body weight (g)	Ending body weight (g)
					Control group	10	388±35	420±53	424±43
Low dose	10	389±33	423±46	421±42
					Middle dose	10	390±33	422±37	418±45
High dose	10	389±32	422±38	420±37

As can be seen from Table 4, there was no significant difference in body weight ((P >0.05) among rats in the early, middle and late stages of the experiment, so that the present formulation had no significant effect on the body weight of rats.

2.2 determination of MAD

As can be seen from Table 5, the MDA content in the serum of the rats in the high and medium dose groups is obviously lower than that in the control group (P <0.01 or P < 0.05). The pharmaceutical composition has the function of reducing the serum MDA level of rats.

TABLE 5 Effect on rat serum MDA levels

Group of	Dosage (mg/kg)	Number of animals	Serum MDA (nmol/ml)
				Control group	0	10	7.32±0.73
Low dose group	200	10	6.82±0.62
				Middle dose group	400	10	6.33±0.88*
High dose group	1200	10	6.01±0.89**

Note: "# compared to control, P <0.05," # compared to control, P < 0.01.

2.3 liver lipofuscin determination

As can be seen from Table 6, the liver content of the rats in the high and medium dose groups was significantly lower than that in the control group (P < 0.01).

TABLE 6 Effect on liver tissue lipofuscin levels in rats

Group of	Dosage (mg/kg)	Animal number (only)	Lipofuscin (microgram/gram tissue)
				Control group	0	10	1.13±0.34
Low dose group	200	10	1.06±0.29
				Middle dose group	400	10	0.52±0.27**
High dose group	1200	10	0.56±0.31**

Note: ". compared to control group, P <0.01

The pharmaceutical composition has the function of reducing the lipofuscin level in the liver of rats.

3.3 determination of superoxide dismutase (SOD) Activity

As can be seen from Table 7, the serum SOD activity of the rats in the high, medium and low dosage forms is significantly higher than that in the control group (P <0.05 or P < 0.01). The medicine formula has the function of increasing the SOD activity of the serum of the rat.

TABLE 7 Effect on rat serum SOD Activity

Group of	Dosage (mg/kg)	Animal number (only)	Serum SOD(NU/ml)
				Control group	0	10	352±95
Low dose group	200	10	441±51*
				Middle dose group	400	10	523±148*
High dose group	1200	10	557±153**

Note: "x" indicates P <0.05 compared to the control group, "x" indicates P <0.01 compared to the control group.

3.4 glutathione peroxidase (GSH-Px) Activity assay

TABLE 8 Effect on serum GSH-Px Activity in rats

Group of	Dosage (mg/kg)	Number of animals (only))	GSH-Px (vitality unit)
				Control group	0	10	425±2
Low dose group	200	10	427±5
				Middle dose group	400	10	428±4
High dose group	1200	10	426±2

As can be seen from Table 8, the activity of serum GSH-Px of rats in each dose group is not significantly different from that of the control group (P > 0.05).

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications and equivalent variations of the above embodiments according to the technical spirit of the present invention are included in the scope of the present invention.

Claims

1. The composition for prolonging life and delaying aging of Dongshan is characterized by comprising the following raw materials in parts by weight: 106 portions of gynostemma pentaphylla, 107 portions of prepared fleece flower root, 113 portions of prepared fleece flower root, 58 to 62 portions of fructus cannabis, 107 portions of rhizoma polygonati, 113 portions of rhizoma gastrodiae, 28 to 32 portions of rhizoma gastrodiae, 58 to 62 portions of polygonatum, 28 to 32 portions of ganoderma lucidum, 48 to 52 portions of eucommia bark, 19 to 21 portions of liquorice, 106 portions of euonymus fortunei, 114 portions of tinospora, 108 portions of paniculate swallowwort root, 112 portions of crataegus leaves and 108 portions of large-fruit hawthorn leaves.

2. The composition for prolonging life and delaying aging according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 110 parts of gynostemma pentaphylla, 110 parts of prepared fleece flower root, 60 parts of fructus cannabis, 110 parts of rhizoma polygonati, 30 parts of rhizoma gastrodiae, 50 parts of radix polygonati officinalis, 30 parts of ganoderma lucidum, 50 parts of eucommia bark, 20 parts of liquorice, 110 parts of euonymus fortune, 110 parts of radix tinosporae, 110 parts of paniculate swallowwort root and 110 parts of big fruit hawthorn leaf.

3. The composition for prolonging life and delaying aging according to claim 1, further comprising 58-62 parts of longan aril and 110-115 parts of shiitake mushroom.

4. A method for preparing the composition for prolonging life and delaying senility as claimed in any one of claims 1 to 3, which comprises the following steps:

5. The method for preparing the composition for prolonging life and delaying aging according to claim 4, wherein the dry powder of rhizoma Gastrodiae, the extract of fructus crataegi of Euonymus fortunei, the enzymatic extract, and the extract of the mixture are combined to make into oral liquid, granules, capsules or tablets.

6. The method for preparing the Dongshan longevity-prolonging senescence-delaying composition according to claim 5, wherein the oral liquid is prepared by adding 16-20 parts by weight of sucrose syrup and 0.06-0.08 part by weight of sodium benzoate into the prepared ganoderma lucidum, gastrodia elata, dried longan pulp and lentinus edodes powder, wintercreeper and hawthorn extract, the enzymatic extract and the mixture extract, adding water to the total weight of the oral liquid to be 1000 parts, boiling, filtering, filling and sterilizing.

7. The method for preparing the composition for prolonging life and delaying aging according to claim 4, wherein in the step (3), the dosage of the cellulase is 0.2-0.3% of the total weight of the tinospora root and the paniculate swallowwort root, and the dosage of the pectinase is 0.1-0.2% of the total weight of the tinospora root and the paniculate swallowwort root.

8. The method for preparing the composition for prolonging life and delaying aging according to claim 4, wherein in the step (1), the Ganoderma lucidum is pulverized by placing the Ganoderma lucidum into a nanometer ultramicro pulverizer to obtain a particle size of 300-500 meshes.

9. The method for preparing the composition for prolonging life and delaying aging according to claim 4, wherein the ultrasonic wall breaking operation time in the step (1) is 15-30 minutes.