CN110691854A - 一种核酸测序方法以及一种核酸测序试剂盒 - Google Patents
一种核酸测序方法以及一种核酸测序试剂盒 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
一种核酸测序方法和一种核酸测序试剂盒，所述试剂盒包含核酸探针，连接酶，3'端连接有阻断基团的dNTP，聚合酶，用于切除连接在dNTP的3'端的阻断基团的试剂1，和用于切除核酸探针上未与被测碱基结合的其余核苷酸的试剂2。
Description
PCT国内申请，说明书已公开。
Claims (26)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017110077817 | 2017-10-25 | ||
| CN201711007781 | 2017-10-25 | ||
| PCT/CN2018/109992 WO2019080725A1 (zh) | 2017-10-25 | 2018-10-12 | 一种核酸测序方法以及一种核酸测序试剂盒 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110691854A true CN110691854A (zh) | 2020-01-14 |
| CN110691854B CN110691854B (zh) | 2023-09-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201880034908.0A Active CN110691854B (zh) | 2017-10-25 | 2018-10-12 | 一种核酸测序方法以及一种核酸测序试剂盒 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US11649489B2 (zh) |
| CN (1) | CN110691854B (zh) |
| WO (1) | WO2019080725A1 (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023035108A1 (zh) * | 2021-09-07 | 2023-03-16 | 深圳华大智造科技股份有限公司 | 一种用于分析靶多核苷酸的序列的方法 |
| WO2023035110A1 (zh) * | 2021-09-07 | 2023-03-16 | 深圳华大智造科技股份有限公司 | 一种用于分析靶多核苷酸的序列的方法 |
| WO2023240494A1 (zh) * | 2022-06-15 | 2023-12-21 | 深圳华大智造科技股份有限公司 | 测序缓冲液及提高具有可逆阻断基团修饰的dNTP的稳定性的方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11993813B2 (en) * | 2017-08-31 | 2024-05-28 | Mgi Tech Co., Ltd. | Nucleic acid probe and nucleic acid sequencing method |
| WO2023240564A1 (zh) * | 2022-06-16 | 2023-12-21 | 深圳华大智造科技股份有限公司 | 含甘氨酸-NaOH缓冲液的切除试剂及其在核酸测序中的应用 |
| WO2024093421A1 (zh) * | 2022-10-31 | 2024-05-10 | 深圳市真迈生物科技有限公司 | 表面处理方法、测序方法和试剂盒 |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030207295A1 (en) * | 1999-04-20 | 2003-11-06 | Kevin Gunderson | Detection of nucleic acid reactions on bead arrays |
| US20090170724A1 (en) * | 2001-12-04 | 2009-07-02 | Shankar Balasubramanian | Labelled nucleotides |
| CN101633961A (zh) * | 2009-08-14 | 2010-01-27 | 东南大学 | 循环“连接－延伸”基因组测序法 |
| CN101910410A (zh) * | 2007-10-23 | 2010-12-08 | 斯特拉托斯基因公司 | 通过间隔进行高通量核酸测序 |
| US20110039259A1 (en) * | 2007-10-19 | 2011-02-17 | Jingyue Ju | Dna sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| CN102030792A (zh) * | 2009-09-29 | 2011-04-27 | 韩国科学技术研究院 | 3＇-ｏ-荧光修饰的核苷酸及其用途 |
| CN104520443A (zh) * | 2012-06-11 | 2015-04-15 | 赛昆塔公司 | 使用序列标签的序列确定方法 |
| CN104854121A (zh) * | 2012-12-20 | 2015-08-19 | 霍夫曼-拉罗奇有限公司 | 用于核酸测序分析的标记的寡核苷酸探针 |
| CN105722850A (zh) * | 2013-08-19 | 2016-06-29 | 雅培分子公司 | 核苷酸类似物 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080242560A1 (en) | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
| CN108165618B (zh) * | 2017-12-08 | 2021-06-08 | 东南大学 | 一种包含核苷酸和3’端可逆封闭核苷酸的dna测序方法 |
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2018
- 2018-10-12 CN CN201880034908.0A patent/CN110691854B/zh active Active
- 2018-10-12 WO PCT/CN2018/109992 patent/WO2019080725A1/zh active Application Filing
- 2018-10-12 US US16/757,719 patent/US11649489B2/en active Active
Patent Citations (9)
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| WO2023240494A1 (zh) * | 2022-06-15 | 2023-12-21 | 深圳华大智造科技股份有限公司 | 测序缓冲液及提高具有可逆阻断基团修饰的dNTP的稳定性的方法 |
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| US20210363576A1 (en) | 2021-11-25 |
| WO2019080725A1 (zh) | 2019-05-02 |
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