CN110664541A

CN110664541A - Preparation method of 3D-printed wound customized band-aid

Info

Publication number: CN110664541A
Application number: CN201910908616.1A
Authority: CN
Inventors: 许太林; 何学成; 张学记
Original assignee: University of Science and Technology Beijing USTB
Current assignee: University of Science and Technology Beijing USTB
Priority date: 2019-09-25
Filing date: 2019-09-25
Publication date: 2020-01-10

Abstract

The invention belongs to the field of sanitary products, and particularly relates to a preparation method of a 3D-printed wound bandage with customized wound, which is matched with the size and shape of an traumatic wound and the wound of an injured part. The wound customization program comprises the steps of collecting image information of the shape and the injured part of the wound, and generating a model file by computer image recognition and modeling so as to match the shape, the size and the injured part of the wound; and 3D printing the model file to generate the base material of the band-aid. The adhesive bandage base material can integrate one or more functions of antibiosis, blood coagulation, moisture preservation, ventilation, inflammation diminishing and the like to accelerate wound healing; the wound customized woundplast printed in 3D mode provided by the invention can be attached to different wounded parts and can be matched with the outline and size of a wound. The shape of the adhesive bandage is adjustable, the medicine application is accurate, the manufacture is simple, the operation is convenient and fast, and the adhesive bandage is particularly suitable for the accurate treatment of trauma wounds with different shapes and different positions.

Description

Preparation method of 3D-printed wound customized band-aid

Technical Field

The invention belongs to the field of sanitary products, and particularly relates to a preparation method of a 3D-printed wound bandage with customized wound, which is matched with the size and shape of an traumatic wound and the wound of an injured part.

Background

The skin is the largest organ of the human body and is the primary barrier isolating the internal environment of the human body from the external environment. Wounds (particularly surgical wounds) are the rapid reactions of the human body after injuries such as cuts, lacerations and the like. Effective diagnostic and therapeutic measures are taken, which are crucial to reducing mortality and morbidity from wound infections: the wound is effectively and timely treated, and can be quickly healed; on the contrary, the patients may be suppurative and infected, and even cause systemic infection, which endangers life. The use of wound dressings is an effective method of treating wounds. As a mature commercial wound dressing product, the medical adhesive bandage can play the basic functions of diminishing inflammation, stopping bleeding and protecting wounds of the wounds, and is convenient, effective and quick. However, the size and the style of the existing band-aid are quite monotonous, the band-aid is not matched with the shape of a wound or cannot take all the wounds into consideration, and part of the wound surface is still exposed; or over-covering the wound, causing the skin surrounding the wound to turn white and soft and secondary infections. In addition, the wound dressings on the market have single appearance, and for wounds of some special parts such as heels, elbows, fingers and the like, the wound dressings in common shapes are difficult to be completely fixed and attached, so that accurate and effective treatment effects cannot be realized.

The 3D printing technology can directly generate entities with any geometric shapes by utilizing a pre-designed computer digital model file in a layer-by-layer printing mode without an original blank or a model, thereby greatly shortening the production period of products, effectively improving the production rate and reducing the production cost. In recent years, with the continuous increase of precise and personalized medical requirements, the 3D printing technology plays an increasingly important role in the medical field. For example, 3D printing has been widely used to customize medical devices (hearing aid housings, complex surgical devices and 3D printed pharmaceuticals), human organs (teeth, blood vessels, liver, muscle tissue). It has become possible to use 3D printed wound dressings for wound care of specific shapes and sizes. The Chinese invention (application No. 201710340553.5) introduces a bandage printer which can print out a bandage at any time by drawing a required shape on a display screen when the bandage is needed. However, the hand-painted and printed band-aid cannot well match the real wound shape and the outline of the injured part, and a great improvement space exists.

Disclosure of Invention

In order to solve the problems, the invention provides a 3D printed wound customized band-aid. The adhesive bandage printed by the 3D printed adhesive bandage has the advantages of integration, high wound matching degree, accurate administration and the like, and an efficient and feasible scheme is provided for accurate and personalized nursing of wounds. The invention has the advantages that the woundplast which is fit with the wound shape and the wound part is printed in a customized manner, unnecessary wound exposure or covering is reduced, and meanwhile, the woundplast can be more closely attached to the wound part and can be printed for use at any time.

The invention is realized by the following technical scheme: a preparation method of a 3D printed wound customized band-aid specifically comprises the following steps:

s1) acquiring wound image information or an image of the injured part;

s2) processing the image information acquired in S1) to generate a three-dimensional model matched with the corresponding wound shape;

s3) preparing printing paste;

s4) 3D printing the slurry of S3) according to the three-dimensional model of S2) to form an active substrate;

s5) assembling an adhesive back lining layer on the active substrate obtained in the step S4 to obtain the wound customized band-aid.

Further, the specific steps of S1) are: and a scanner or other intelligent equipment is adopted to acquire image data of the wound to be treated, and the acquired image data is acquired and sent to the PC.

Further, the specific steps of S2) are:

s2.1) determining coordinate data of the wound contour by sequentially carrying out black-and-white processing, binarization processing and eight-connectivity method on the received image information, and generating a two-dimensional vector diagram of the wound contour by tracing the obtained coordinate data;

and S2.2) importing the two-dimensional vector diagram for generating the wound outline into the system, generating a three-dimensional model matched with the corresponding wound shape by using an extrusion command, storing the three-dimensional model in an STL file format, storing the three-dimensional model in an SD card, and printing the three-dimensional model.

Further, the specific steps of S3) are:

s3.1) adding the nano particle material subjected to drug modification selection into deionized water to prepare an aqueous solution;

s3.2) adding gelatin into deionized water, heating and carrying out ultrasonic treatment to obtain a gelatin solution;

s3.3) respectively measuring the aqueous solution prepared in the step S3.1) and the gelatin solution obtained in the step S3.2) by using a micro-pipette, pouring the aqueous solution and the gelatin solution into a container, carrying out oil bath and light-proof magnetic stirring at the temperature of 90-110 ℃ for 25-35min, and forming the gelatin solution doped with the nano particles in situ;

s3.4) carrying out ultrasonic treatment on the gelatin solution for 25-35min, and storing the cooled slurry in a dark place for subsequent printing.

Further, the specific steps of S4) are:

s4.1) heating the slurry prepared in the S3 to 33-39 ℃ to be melted, and then sending the melted slurry into a printer to start printing;

and S4.2) gradually cooling the slurry solution after layered printing for re-solidification, accumulating layer by layer to form a mixed solution which is dripped on the model after printing is finished, and obtaining a fully solidified active base material after 15-25 min.

Further, the specific steps of S5) are:

s5.1) selecting a backing layer according to the wound type, and fixing the backing layer on an inner cavity of the PCL backing layer;

s5.2) transferring and fixing the active substrate prepared in the step S4) on the inner cavity of the PCL backing layer to complete the assembly.

Further, the mixed solution is 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride and an ethanol solution of N-hydroxysuccinimide in a mass ratio of 5: 2.

further, the wound determination in S5) includes: for wounds with a common flat part, the adhesive backing layer is one or more of a pressure-sensitive bandage, an adhesive plaster and a film; for a wound site with a complex curved surface structure, the adhesive backing layer is generated by 3D printing after scan modeling of the wound site.

Further, the nanoparticle material in S3.1) is a material with one or more functions of sterilization, blood coagulation, moisture retention, air permeability and inflammation diminishing, including but not limited to silver nitrate, titanium dioxide, benzalkonium chloride and hyaluronic acid.

The wound customized band-aid is prepared by adopting the method.

The invention has the beneficial effects that: due to the adoption of the technical scheme, the 3D-printed wound customized band-aid provided by the invention can be attached to different injured parts and can be matched with the outline and size of a wound. The shape of the adhesive bandage is adjustable, the medicine application is accurate, the manufacture is simple, the operation is convenient and fast, and the adhesive bandage is particularly suitable for the accurate treatment of trauma wounds with different shapes and different positions.

Drawings

The invention will be further explained with reference to the drawings

Fig. 1 is a schematic view of wound identification provided in an embodiment of the present invention.

FIG. 2 is an image identification and modeling diagram of different wound shapes provided by an embodiment of the present invention.

Fig. 3 is a schematic view of a 3D printed woundplast substrate according to an embodiment of the invention.

Fig. 4 is a graph showing the bactericidal effect of the 3D printed gelatin-based hydrogel substrate with moisturizing function provided by the embodiment of the invention.

Fig. 5 is a graph showing the swelling ratio of a 3D-printed gelatin-based hydrogel with bactericidal function according to an embodiment of the present invention as a function of time.

Fig. 6 is a schematic view of a 3D printed fingertip-fitted adhesive backing layer provided by an embodiment of the present invention.

Fig. 7 is a schematic view illustrating that the 3D printed band-aid provided by the embodiment of the invention is firmly attached to a fingertip.

Detailed description of the preferred embodiments

In order to facilitate the technical means, creation features, achievement of the purpose and the efficacy of the invention, the invention is further explained by combining the specific implementation examples. It should be noted that the present invention is not limited by the above-mentioned embodiments, and the embodiments and descriptions are only for illustrating the principle of the present invention, and various changes and modifications of the present invention are possible without departing from the spirit and scope of the present invention, which fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

The invention relates to a preparation method of a 3D-printed wound customized woundplast, which specifically comprises the following steps:

s1) acquiring wound image information or an image of the injured part;

s3) preparing printing paste;

s5) assembling an adhesive back lining layer on the active substrate obtained in the step S4), and obtaining the wound customized band-aid.

Further, the specific steps of S1) are: and a scanner or other intelligent equipment is adopted to collect image information of the wound to be treated, and the collected image information is sent to the PC.

Further, the specific steps of S2) are:

Further, the specific steps of S3) are:

s3.3) respectively measuring the aqueous solution prepared in the S3.1) and the gelatin solution obtained in the S3.2) and pouring the aqueous solution and the gelatin solution into a container, and carrying out oil bath light-proof magnetic stirring at the temperature of 90-110 ℃ for 25-35min to form the gelatin solution doped with the nano particles in situ;

Further, the specific steps of S4) are:

s4.1) heating the gelatin solution prepared in the step S3 to 33-39 ℃ to melt, and then sending the gelatin solution into a printer to start printing;

s4.2) gradually cooling the gelatin solution after layered printing for re-solidification, accumulating layer by layer to form a mixed solution which is dripped on the model after printing is finished, and obtaining a fully solidified active base material after 15-25 min.

Further, the specific steps of S5) are:

The wound customized band-aid is prepared by adopting the method.

Embodiment 13D printing an arm-attached adhesive bandage

Step 1: image identification and modeling of wounds

Firstly, three mouths with different shapes are cut on the pigskin, a scanner or a smart phone is used for shooting a picture (shown in figure 1) on the front face of the wound, and the picture is wirelessly transmitted to a PC terminal. And the PC determines the coordinate data of the wound contour through black and white processing, binarization processing and an eight-connectivity method in sequence, then introduces Adobe illustrator (Ai) software, runs a customized Ai plug-in, and then automatically traces to generate a two-dimensional vector diagram of the wound contour. After the Autodesk 3ds max software is further introduced, the outline size can be modified appropriately, a three-dimensional model (as shown in FIG. 2) matching the corresponding wound shape is generated by an extrusion command, and the three-dimensional model is stored in an STL file format and then is printed on an SD card.

Step 2: preparation of printing paste-silver nanoparticle modified gelatin-based hydrogel

Pouring 0.1g of silver nitrate into 9.9mL of deionized water to prepare a silver nitrate solution with the mass fraction of 1%; weighing 1g of gelatin, pouring the gelatin into 9mL of deionized water, heating the gelatin at 37 ℃ until the gelatin is completely dissolved, and carrying out ultrasonic treatment for 30min to form a gelatin solution with the mass fraction of 10%. Respectively measuring 500 mul of silver nitrate with the mass fraction of 1% and 4500 mul of gelatin solution with the mass fraction of 10% by using a micro liquid transfer gun, pouring the silver nitrate and the 4500 mul of gelatin solution into a small beaker, and carrying out oil bath and light-proof magnetic stirring for 30min at the temperature of 100 ℃ to form the gelatin solution doped with the Ag nano particles in situ. The solution is subjected to ultrasonic treatment for 30min to ensure that the slurry is free from agglomeration, layering and uniformity. And storing the cooled slurry in dark for subsequent printing.

And step 3: gelatin-based hydrogel base material with moisturizing and sterilizing functions for 3D printing

Heating a gelatin solution with the mass fraction of 10% to 37 ℃ to melt, and then sending the gelatin solution into a printer to start printing. And gradually cooling the gelatin solution after layered printing for re-solidification, accumulating layer by layer to form a solution after printing is finished, dropwise adding a certain amount of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (EDC-NHS) ethanol solution onto the model, and obtaining a completely solidified substrate after 20min (as shown in figure 3).

And 4, step 4: verification of moisturizing effect of gelatin substrate

Hydrogel-based materials generally swell significantly in water and therefore can provide some wound moisturizing effect. And (3) freezing the printed gelatin substrate in a refrigerator at the temperature of-80 ℃ for 24h, immediately placing the gelatin substrate in a freeze dryer, and taking out the gelatin substrate after freeze drying for 72 h. The dry weight of the gelatin substrate was recorded. Putting gelatin in deionized water for 5min, taking out, drying surface water with filter paper, and sequentially recording the weight of water absorbed for 5, 10, 15, 20, 40 and 60 min. The swelling ratio was plotted against time by dividing the weight after water absorption by the dry weight, as shown in FIG. 4. The result shows that the gelatin base material has an ultrahigh swelling rate of 200%, is beneficial to partial moisture retention of the wound and promotes wound healing.

And 5: verification of the Sterilization Effect of the gelatin base Material

Staphylococcus aureus was selected as a model strain. Suspending Staphylococcus aureus to 10³CFU mL^-1. 0.4mL of the microorganism suspension was uniformly spread in LB medium using a sterile glass spreader. The printed circular hydrogel sterilization substrate (diameter 2cm) is lightly placed on an LB culture medium and is placed in a 37 ℃ thermostat for 24 hours. As shown in fig. 5, bacterial growth around the hydrogel was significantly inhibited, showing a certain bactericidal effect.

Step 6: assembly of adhesive backing layer

Because the injured part is the arm, comparatively flat, directly choose for use elastic textile as the back sheet, directly transfer the hydrogel substrate after the solidification and fix to this back sheet, accomplish the equipment.

Example 23D printing fingertip-attached Woundplast

Step 1: wound and fingertip scanning and modeling

The process of scan modeling of the wound was essentially the same as in example 1: first, a round opening is cut on the tip of the index finger of the dummy human body model. And (3) respectively shooting photos of the front of the wound by using a smart phone, and automatically tracing to generate a two-dimensional vector diagram of the wound outline after running the customized Ai plug-in. After the Autodesk 3ds max software is further introduced, the outline size can be modified appropriately, a three-dimensional model is generated by an extrusion command, and the three-dimensional model is stored in an STL file format and then is printed in an SD card. Scanning and modeling process of the injured part: the method comprises the steps of taking a photo by using a smart phone facing the front of an injured index finger, superposing and converting the taken photo by an image converter and a transmission converter into a three-dimensional image, further guiding the three-dimensional image into Autodesk 3ds max software, properly modifying the outline size to generate a three-dimensional model, storing the three-dimensional model in an STL file format, storing the three-dimensional model in an SD card, and printing the three-dimensional model.

Step 2: preparation of printing raw materials-hydrogel of silver nanoparticle modified gelatin base and Polycaprolactone (PCL)

The preparation process of the silver nanoparticle-modified gelatin-based hydrogel was the same as in example 1. The adhesive back layer adopts Polycaprolactone (PCL) as a raw material, and the preparation process comprises the following steps: adding 27g of PCL into 100mL of dichloromethane, heating and stirring at 40 ℃ for 30min until the PCL is completely dispersed; the solvent was evaporated using a rotary evaporator at 40 ℃ for 3h and dried under high vacuum for 1 h. And cutting the PCL material after freeze-drying into small pieces and storing.

And step 3: 3D prints substrate and back sheet

Printing of the base material: the printing process of the silver nanoparticle-modified gelatin-based hydrogel was the same as in example 1. PCL-based adhesive backing layer printing process was as follows: and heating the PCL dry matter to 75 ℃ again to be melted, and sending the PCL dry matter into a printer to start printing. The PCL after layered printing is gradually cooled and re-solidified, and the PCL is cumulatively printed layer by layer to form a semi-closed inner cavity in a finger shape, as shown in FIG. 6.

And 4, step 4: assembly of adhesive backing layer

The cured gelatin-based hydrogel substrate was carefully transferred and fixed to the inner lumen of the PCL backing layer, assembled, and fitted to the injured finger site as shown in fig. 7.

While several embodiments of the present invention have been presented herein, it will be appreciated by those skilled in the art that changes may be made to the embodiments herein without departing from the spirit of the invention. The above examples are merely illustrative and should not be taken as limiting the scope of the invention.

Claims

1. A preparation method of a 3D printed wound customized band-aid is characterized by comprising the following steps:

s1) acquiring image information of the wound or the injured part;

s3) preparing printing paste;

2. The preparation method according to claim 1, wherein the specific steps of S1) are as follows: and a scanner or other intelligent equipment is adopted to collect image information of the wound to be treated, and the collected image information is sent to the PC.

3. The preparation method according to claim 2, wherein the specific steps of S2) are as follows:

4. The preparation method according to claim 1, wherein the specific steps of S3) are as follows:

5. The preparation method according to claim 1, wherein the specific steps of S4) are as follows:

s4.1) heating the slurry prepared in the step S3) to 33-39 ℃ to be melted, and then sending the melted slurry into a printer to start printing;

6. The preparation method according to claim 5, wherein the specific steps of S5) are as follows:

7. The preparation method according to claim 5, wherein the mixed solution is a solution of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide in ethanol in a mass ratio of 5: 2.

8. the preparation method according to claim 6, wherein the wound determination in S5) comprises the following specific steps: for wounds with flat parts, the adhesive backing layer is one or more of a pressure-sensitive bandage, an adhesive tape and a film; for a wound site with a complex curved surface structure, the adhesive backing layer is generated by 3D printing after scan modeling of the wound site.

9. The preparation method according to claim 4, wherein the nanoparticle material in S3.1) is a material with one or more functions of sterilization, blood coagulation, moisture retention, air permeability and inflammation diminishing, and includes but is not limited to silver nitrate, titanium dioxide, benzalkonium chloride and hyaluronic acid.

10. A wound-customized adhesive bandage, characterized in that it is prepared by the method according to any one of claims 1-9.