CN110664541A - Preparation method of 3D-printed wound customized band-aid - Google Patents

Abstract

The invention belongs to the field of sanitary products, and in particular relates to a preparation method of a 3D-printed wound-customized band-aid that fits the size, shape and wound site of a traumatic wound. The wound customization program includes image information collection of wound shape and injured part, computer image recognition and modeling to generate a model file to match the shape, size and injured part of the wound; the model file is 3D printed to generate a Band-Aid base material. The Band-Aid base material can integrate one or more functions such as antibacterial, coagulation, moisturizing, ventilation, and anti-inflammatory to accelerate wound healing; the 3D-printed wound-customized Band-Aid provided by the present invention can fit different wounds. It can also fit the contour and size of the wound. The shape of the band-aid is adjustable, the dressing is precise, the production is simple, convenient and quick, and it is especially suitable for the precise treatment of traumatic wounds of different shapes and parts.

Description

A preparation method of a 3D printed wound customized Band-Aid

technical field

The invention belongs to the field of sanitary products, and in particular relates to a preparation method of a 3D-printed wound-customized band-aid that fits the size, shape and wound site of a traumatic wound.

Background technique

The skin is the largest organ of the human body and is the primary barrier to isolate the human body's internal environment from the external environment. Wounds (especially surgical wounds) are the rapid response of the human body to skin cuts, tears and other injuries. Taking effective diagnosis and treatment measures is essential to reduce the mortality and morbidity caused by wound infection: effective and timely wound treatment can make it heal quickly; Systemic infection, life-threatening. Using wound dressings is an effective way to manage wounds. As a mature commercial wound dressing product, medical Band-Aid can play the basic functions of wound anti-inflammatory, hemostasis and wound protection, which is convenient, effective and fast. But existing Band-Aids are drab in size and style, don't match the shape of the wound—or don't cover the entire wound, leaving part of the wound exposed; or cover the wound too much, leaving the surrounding skin blanching, soft, and secondary Infect. In addition, the shape of the band-aids on the market is single. For some special wounds such as heels, elbows, fingers, etc., it is difficult to completely fix and fit the ordinary-shaped band-aids, so accurate and effective treatment effects cannot be achieved.

3D printing technology can use pre-designed computer digital model files to directly generate entities of any geometric shape through layer-by-layer printing, without the need for original embryos or models, thus greatly shortening the production cycle of products, effectively improving productivity, reducing production cost. In recent years, with the continuous growth of precision and personalized medical needs, 3D printing technology has played an increasingly important role in the medical field. For example, 3D printing is already widely used to customize medical devices (hearing aid housings, complex surgical instruments and 3D printed medicines), human organs (teeth, blood vessels, liver, muscle tissue). It has become possible to use 3D printed Band-Aids for wound care of specific shapes and sizes. China Invention (Application No. 201710340553.5) introduces a Band-Aid printer. When you need to use a Band-Aid, you can draw the shape you want on the display screen, and you can print it at any time. However, such hand-printed Band-Aids often do not match the real wound shape and contour of the injured part well, and there is a lot of room for improvement.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention proposes a 3D printed wound customized Band-Aid. The 3D-printed Band-Aid The printed Band-Aid has the advantages of integration, high wound matching, precise drug administration, etc., and provides an efficient and feasible solution for precise and personalized wound care. The beneficial effect of the present invention is that the Band-Aid can be customized to fit the shape of the wound and the wound site, so as to reduce unnecessary exposure or coverage of the wound, and at the same time, it can fit the injured site more closely, and can be printed and used at any time.

The present invention is realized by the following technical solutions: a preparation method of a 3D printed wound customized Band-Aid, the method specifically comprises the following steps:

S1) collecting wound image information or an image of the injured part;

S2) processing the image information collected in S1) to generate a three-dimensional model matching the corresponding wound shape;

S3) prepare printing paste;

S4) 3D printing the slurry of S3) according to the three-dimensional model of S2) to form the active substrate;

S5) assembling the adhesive backing layer on the active substrate obtained in S4, that is, to obtain a wound-customized Band-Aid.

Further, the specific steps of S1) are: using a scanner or other intelligent equipment to collect image data from the wound to be treated, and sending the collected image data collection to the PC.

Further, the concrete steps of described S2) are:

S2.1) the received image information is processed successively through black and white processing, binarization processing and the eight-connection method to determine the coordinate data of the wound contour, and the two-dimensional vector diagram of the wound contour is generated by obtaining the coordinate data tracing;

S2.2) After importing the two-dimensional vector diagram of the generated wound contour into the system, use the extrusion command to generate a three-dimensional model matching the corresponding wound shape, save it as an STL file format and store it in the SD card and print it.

Further, the concrete steps of described S3) are:

S3.1) adding the nanoparticle material after drug modification and selection into deionized water to make an aqueous solution;

S3.2) adding gelatin into deionized water, heating and ultrasonic treatment to obtain gelatin solution;

S3.3) Use a micropipette to measure the aqueous solution prepared in S3.1) and the gelatin solution obtained in S3.2) and pour it into a container, and stir in an oil bath at a temperature of 90-110°C for 25-35 min in the dark in an oil bath. , in situ formation of a gelatin solution doped with nanoparticles;

S3.4) After the gelatin solution is ultrasonically treated for 25-35 minutes, the cooled slurry is stored in the dark for subsequent printing.

Further, the concrete steps of described S4) are:

S4.1) The slurry prepared in S3 is heated to 33-39 ℃ and melted, and then sent to the printer to start printing;

S4.2) The slurry solution after layered printing is gradually cooled and re-solidified. After the printing is completed layer by layer, the mixed solution is dripped onto the model, and a fully cured active substrate is obtained after 15-25 minutes.

Further, the concrete steps of described S5) are:

S5.1) root wound type, select the backing layer, and fix it on the inner cavity of the PCL backing layer;

S5.2) The active substrate prepared in S4) is transferred and fixed to the inner cavity of the PCL backing layer to complete the assembly.

Further, the mixed solution is 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide ethanol solution, and the mass ratio of the two is 5:2.

Further, the specific steps for determining the wound in the described S5) are: for the wound in the common flat part, the adhesive backing layer selects one or more of pressure-sensitive bandages, adhesive tapes, and films; for wounds with complex curved surface structures The adhesive backing layer was created by 3D printing after scanning and modeling the injured area.

Further, the nanoparticle material in S3.1) is a material with one or more functions of sterilization, blood coagulation, moisture retention, ventilation, and anti-inflammatory, including but not limited to silver nitrate, titanium dioxide, benzalkonium chloride, hyaluronic acid acid.

A wound-customized Band-Aid, the wound-customized Band-Aid is prepared by the above method.

The beneficial effects of the present invention are: due to the adoption of the above technical solutions, the 3D printed wound customized Band-Aid provided by the present invention can not only fit different injured parts, but also fit the contour and size of the wound. The shape of the band-aid is adjustable, the dressing is precise, the production is simple, convenient and quick, and it is especially suitable for the precise treatment of traumatic wounds of different shapes and parts.

Description of drawings

The present invention will be further described below in conjunction with the accompanying drawings

FIG. 1 is a schematic diagram of wound identification according to an embodiment of the present invention.

FIG. 2 is an image recognition and modeling diagram of different wound shapes provided by an embodiment of the present invention.

FIG. 3 is a schematic diagram of a 3D printing Band-Aid substrate provided in an embodiment of the present invention.

FIG. 4 is a diagram showing the sterilization effect of the 3D printed gelatin-based hydrogel substrate with moisturizing function provided by the embodiment of the present invention.

FIG. 5 is a graph of the swelling ratio of the 3D printed gelatin-based hydrogel with bactericidal function provided by an embodiment of the present invention as a function of time.

FIG. 6 is a schematic diagram of a 3D printed adhesive backing layer that fits a fingertip according to an embodiment of the present invention.

FIG. 7 is a schematic diagram of the 3D printed Band-Aid being firmly attached to the fingertip according to an embodiment of the present invention.

specific implementation

In order to facilitate the technical means, creation features, achievement of purposes and effects of the present invention, the present invention is further described below in conjunction with specific implementation cases. It is worth noting that the present invention is not limited by the above-mentioned embodiments, and the descriptions in the embodiments and the specification are only to illustrate the principle of the present invention, and the present invention may have various changes and improvements without departing from the spirit and scope of the present invention. , these changes and improvements fall within the scope of the claimed invention. The claimed scope of the present invention is defined by the appended claims and their equivalents.

The present invention is a preparation method of a 3D printed wound customized Band-Aid, the method specifically comprises the following steps:

S1) collecting wound image information or an image of the injured part;

S2) processing the image information collected in S1) to generate a three-dimensional model matching the corresponding wound shape;

S3) prepare printing paste;

S4) 3D printing the slurry of S3) according to the three-dimensional model of S2) to form the active substrate;

S5) Assembling the adhesive backing layer on the active substrate obtained in S4), that is, to obtain a wound-customized Band-Aid.

Further, the specific steps of S1) are: using a scanner or other intelligent equipment to collect image information of the wound to be treated, and sending the collected image information to the PC.

Further, the concrete steps of described S2) are:

S2.1) the received image information is processed successively through black and white processing, binarization processing and the eight-connection method to determine the coordinate data of the wound contour, and the two-dimensional vector diagram of the wound contour is generated by obtaining the coordinate data tracing;

S2.2) After importing the two-dimensional vector diagram of the generated wound contour into the system, use the extrusion command to generate a three-dimensional three-dimensional model matching the corresponding wound shape, save it as an STL file format and store it in the SD card and then print it.

Further, the concrete steps of described S3) are:

S3.1) adding the nanoparticle material after drug modification and selection into deionized water to make an aqueous solution;

S3.2) adding gelatin into deionized water, heating and ultrasonic treatment to obtain gelatin solution;

S3.3) respectively measure the aqueous solution prepared in S3.1) and the gelatin solution obtained in S3.2) and pour it into a container, and stir in an oil bath at a temperature of 90-110°C for 25-35min in the dark and magnetic force to form a dopant in situ. Gelatin solution with nanoparticles;

S3.4) After the gelatin solution is ultrasonically treated for 25-35 minutes, the cooled slurry is stored in the dark for subsequent printing.

Further, the concrete steps of described S4) are:

S4.1) heating the gelatin solution prepared in S3 to 33-39 ℃ and melting and then sending it into the printer to start printing;

S4.2) The gelatin solution after layered printing is gradually cooled and re-solidified. After the printing is completed, the mixed solution is formed layer by layer. After 15-25 minutes, a fully solidified active substrate is obtained.

Further, the concrete steps of described S5) are:

S5.1) root wound type, select the backing layer, and fix it on the inner cavity of the PCL backing layer;

S5.2) The active substrate prepared in S4) is transferred and fixed to the inner cavity of the PCL backing layer to complete the assembly.

Further, the mixed solution is 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide ethanol solution, and the mass ratio of the two is 5:2.

Further, the specific steps for determining the wound in the described S5) are: for the wound in the common flat part, the adhesive backing layer selects one or more of pressure-sensitive bandages, adhesive tapes, and films; for wounds with complex curved surface structures The adhesive backing layer was created by 3D printing after scanning and modeling the injured area.

Further, the nanoparticle material in S3.1) is a material with one or more functions of sterilization, blood coagulation, moisture retention, ventilation, and anti-inflammatory, including but not limited to silver nitrate, titanium dioxide, benzalkonium chloride, hyaluronic acid acid.

A wound-customized Band-Aid, the wound-customized Band-Aid is prepared by the above method.

Example 1 3D printing a band-aid that fits the arm

Step 1: Image recognition and modeling of wounds

First, cut three different shapes of openings on the pigskin, use a scanner or smartphone to take pictures of the front of the wound (as shown in Figure 1), and wirelessly transmit the pictures to the PC. The photo was processed in black and white, binarized, and eight-connected method to determine the coordinate data of the wound contour, and then imported into Adobe illustrator (Ai) software. After running the customized Ai plug-in, the two-dimensional vector diagram of the wound contour was automatically generated by tracing. After further importing into Autodesk 3ds max software, the outline size can be appropriately modified, and the 3D model matching the corresponding wound shape can be generated by the extrusion command (as shown in Figure 2), which is saved as STL file format and stored in the SD card for printing.

Step 2: Printing Paste - Preparation of Silver Nanoparticle-Modified Gelatin-Based Hydrogel

Pour 0.1 g of silver nitrate into 9.9 mL of deionized water to prepare a silver nitrate solution with a mass fraction of 1%; weigh 1 g of gelatin and pour it into 9 mL of deionized water, heat at 37°C until the gelatin is completely dissolved and sonicated for 30 min, the mass fraction is 10% gelatin solution. Use a micropipette to measure 500 μL of silver nitrate with a mass fraction of 1% and 4500 μL of a 10% gelatin solution into a small beaker, stir in an oil bath at 100 °C for 30 min in the dark, and form Ag-doped in situ. Gelatin solution of nanoparticles. The solution was ultrasonicated for 30 minutes to ensure that the slurry was free of agglomeration, delamination and uniformity. The cooled paste should be protected from light and used for subsequent printing.

Step 3: 3D Printing Gelatin-Based Hydrogel Substrates with Moisturizing, Bactericidal Functions

The gelatin solution with a mass fraction of 10% was heated to 37°C and melted, and then sent to the printer to start printing. The gelatin solution after layered printing was gradually cooled and re-solidified. After the layer-by-layer accumulation was completed, a certain amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid was added dropwise to the model. Salt and N-hydroxysuccinimide (EDC-NHS) ethanol solution, and a fully cured substrate was obtained after 20 min (as shown in Figure 3).

Step 4: Verify the moisturizing effect of the gelatin substrate

Hydrogel-based materials generally swell significantly in water, so they can have a certain wound moisturizing effect. The printed gelatin substrate was placed in a freezer at -80°C for 24 hours, then immediately placed in a freeze dryer, and taken out after freeze drying for 72 hours. The dry weight of the gelatin substrate was recorded. The gelatin was placed in deionized water for 5 minutes and taken out, and the water on the surface was blotted with filter paper, and the weights of water absorbed for 5, 10, 15, 20, 40 and 60 minutes were recorded in turn. By dividing the weight after water absorption by the dry weight, a line graph of the swelling ratio over time was obtained, as shown in Figure 4. The results show that the gelatin substrate has an ultra-high 200% swelling rate, which is beneficial to the local moisturizing of wounds and promotes wound healing.

Step 5: Verify the germicidal effect of the gelatin substrate

Staphylococcus aureus was selected as the model strain. Suspend S. aureus to 10 ³ CFU mL ^-1 . Spread 0.4 mL of the microbial suspension evenly in the LB medium using a sterile glass spreader. The printed circular hydrogel sterilization substrate (2 cm in diameter) was lightly placed on the LB medium and placed in a 37°C incubator for 24 hours. As shown in Figure 5, the bacterial growth around the hydrogel was significantly inhibited, showing a certain bactericidal effect.

Step 6: Assembly of Adhesive Backing Layer

Since the injured part is the arm, which is relatively flat, an elastic textile is directly used as the backing layer, and the cured hydrogel substrate is directly transferred and fixed on the backing layer to complete the assembly.

Example 2 3D printing band-aids that fit the fingertips

Step 1: Scanning and Modeling of Wounds and Fingertips

The scanning and modeling process of the wound is basically the same as the implementation case 1: first, a circular incision is made on the index finger of the dummy mannequin. Take photos of the front of the wound with a smartphone, and run the customized Ai plug-in to automatically trace and generate a two-dimensional vector diagram of the wound outline. After further importing into Autodesk 3ds max software, the outline size can be appropriately modified, and the 3D model can be generated by the extrusion command, which is saved as STL file format and stored in the SD card for printing. Scanning and modeling process of the injured part: Take a photo of the front of the injured index finger with a smartphone. The taken photo is superimposed by an image converter and a transfer converter and converted into a three-dimensional image. After further importing into Autodesk 3ds max software, the outline size can be modified appropriately , generate a three-dimensional model, save it as STL file format, save it to the SD card, and then print it.

Step 2: Printing Raw Materials - Preparation of Silver Nanoparticles-Modified Gelatin-Based Hydrogel and Polycaprolactone (PCL)

The preparation process of the silver nanoparticle-modified gelatin-based hydrogel is the same as that of Example 1. The adhesive backing layer uses polycaprolactone (PCL) as raw material, and the preparation process is as follows: take 27 g of PCL and add 100 mL of dichloromethane, heat and stir at 40 ° C for 30 min until PCL is completely dispersed; the solvent is used in a rotary evaporator It was evaporated at 40°C for 3h and dried under high vacuum for 1h. The lyophilized PCL material was cut into small pieces and stored.

Step 3: 3D printing substrate and backing layer

Printing of the substrate: The printing process of the silver nanoparticle-modified gelatin-based hydrogel is the same as that of Example 1. The printing process of the PCL-based adhesive backing layer is as follows: the dry matter of PCL is reheated to 75°C to melt and then sent into the printer to start printing. The PCL after layer-by-layer printing is gradually cooled and re-solidified, and the layer-by-layer accumulation printing is completed, forming a finger-shaped semi-closed inner cavity, as shown in Figure 6.

Step 4: Assembly of Adhesive Backing Layer

The cured gelatin-based hydrogel substrate was carefully transferred to the inner cavity of the PCL backing layer to complete the assembly and fit to the injured finger, as shown in Figure 7.

Although several embodiments of the present invention have been presented herein, those skilled in the art should understand that changes may be made to the embodiments herein without departing from the spirit of the present invention. The above-mentioned embodiments are only exemplary, and the embodiments herein should not be construed as limiting the scope of the rights of the present invention.

Claims (10)

1. the preparation method of the customized Band-Aid of a 3D printed wound, it is characterised in that the method specifically comprises the following steps: S1) collecting image information of the wound or the injured part; S2) processing the image information collected in S1) to generate a three-dimensional model matching the corresponding wound shape; S3) prepare printing paste; S4) 3D printing the slurry of S3) according to the three-dimensional model of S2) to form the active substrate; S5) assembling the adhesive backing layer on the active substrate obtained in S4, that is, to obtain a wound-customized Band-Aid. 2 . The preparation method according to claim 1 , wherein the specific steps of S1) are: using a scanner or other intelligent equipment to collect image information of the wound to be treated, and sending the collected image information to a PC. 3 . 3. preparation method according to claim 2, is characterized in that, the concrete step of described S2) is: S2.1) the received image information is processed successively through black and white processing, binarization processing and the eight-connection method to determine the coordinate data of the wound contour, and the two-dimensional vector diagram of the wound contour is generated by obtaining the coordinate data tracing; S2.2) After importing the two-dimensional vector diagram of the generated wound contour into the system, use the extrusion command to generate a three-dimensional three-dimensional model matching the corresponding wound shape, save it as an STL file format and store it in the SD card and then print it. 4. preparation method according to claim 1, is characterized in that, the concrete step of described S3) is: S3.1) adding the nanoparticle material after drug modification and selection into deionized water to make an aqueous solution; S3.2) adding gelatin into deionized water, heating and ultrasonic treatment to obtain gelatin solution; S3.3) respectively measure the aqueous solution prepared in S3.1) and the gelatin solution obtained in S3.2) and pour it into a container, and at a temperature of 90-110°C, stir in an oil bath to avoid light and magnetic force for 25-35min, and form a dopant in situ. Gelatin solution with nanoparticles; S3.4) After the gelatin solution is ultrasonically treated for 25-35 minutes, the cooled slurry is stored in the dark for subsequent printing. 5. preparation method according to claim 1, is characterized in that, the concrete step of described S4) is: S4.1) The slurry prepared in S3) is heated to 33-39°C and melted, and then sent to the printer to start printing; S4.2) The slurry solution after layered printing is gradually cooled and re-solidified. After the printing is completed layer by layer, the mixed solution is dripped onto the model, and a fully cured active substrate is obtained after 15-25 minutes. 6. preparation method according to claim 5, is characterized in that, the concrete step of described S5) is: S5.1) root wound type, select the backing layer, and fix it on the inner cavity of the PCL backing layer; S5.2) The active substrate prepared in S4) is transferred and fixed to the inner cavity of the PCL backing layer to complete the assembly. 7. preparation method according to claim 5 is characterized in that, described mixed solution is 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinyl Imine ethanol solution, the mass ratio of the two is 5:2. 8. preparation method according to claim 6, is characterized in that, the wound in described S5) is determined concrete step is: for the wound of flat part, adhesive backing layer selects pressure-sensitive bandage, adhesive cloth, in the film. One or more; for wounded parts with complex curved structures, the adhesive backing layer is generated by 3D printing after scanning and modeling the wounded parts. 9. preparation method according to claim 4 is characterized in that, the nanoparticle material in described S3.1) is the material with one or more functions of sterilization, blood coagulation, moisture retention, ventilation, anti-inflammatory, including but Not limited to silver nitrate, titanium dioxide, benzalkonium chloride, hyaluronic acid. 10. A wound-customized Band-Aid, characterized in that, the wound-customized Band-Aid is prepared by the method according to any one of claims 1-9.

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