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CN103520767A - Anti-microbial healing-promoting hydrogel dressing and preparation method therefor - Google Patents

Anti-microbial healing-promoting hydrogel dressing and preparation method therefor Download PDF

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CN103520767A
CN103520767A CN 201310517301 CN201310517301A CN103520767A CN 103520767 A CN103520767 A CN 103520767A CN 201310517301 CN201310517301 CN 201310517301 CN 201310517301 A CN201310517301 A CN 201310517301A CN 103520767 A CN103520767 A CN 103520767A
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dressing
hydrogel
anti
microbial
healing
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CN 201310517301
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CN103520767B (en )
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徐艳丽
杨学英
董军
丁连珠
刘常琳
黄琳静
孙春明
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山东赛克赛斯药业科技有限公司
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Abstract

The invention discloses an anti-microbial healing-promoting hydrogel dressing and a preparation method therefor. The main ingredients of the dressing are water-soluble high-molecular polymers, functional polymers with pseudoplastic, anti-microbial agents and water. The above ingredients are dissolved into a whole after a series of technologies. The solution is poured into a mold, and the hydrogel dressing is obtained after curing and irradiation disinfection. The results of wound healing experiments and in vitro anti-microbial experiments show that the hydrogel dressing can promote wound healing effectively, and has good bacteriostatic activity to multiple bacteria (the sterilizing rate is above 70%). The anti-microbial healing-promoting hydrogel dressing is better than existing hydrogel dressings obviously, and has advantages of substantial anti-microbial curative effects, transparency, high water content, simple preparation method, pure product, nontoxicity, innocuousness, no stimulation, good biocompatibility and the like. The hydrogel dressing is suitable for large-area promotion and application.

Description

一种抗菌促愈水凝胶敷料及其制备方法 An antimicrobial hydrogel dressing and healing promoting preparation

技术领域 FIELD

[0001] 本发明属于医用创伤敷料及其制备领域,特别是涉及一种适用于烧烫伤、机械性伤口、手术切口及慢性创面保护和治疗的抗菌促愈水凝胶敷料及其制备方法。 [0001] The present invention belongs to the field of medical wound dressings and their preparation, particularly to a suitable burns, mechanical wounds, chronic wounds and surgical incisions antibacterial protection and treatment Cuyu hydrogel dressing and a preparation method.

背景技术 Background technique

[0002] 皮肤是人体的最大器官,是维持体内环境稳定和阻止微生物入侵的天然屏障;然而,大面积暴露于外界的特点,使得皮肤很容易受到烧伤、机械伤等外来伤害。 [0002] The skin is the largest organ of the human body, it is to maintain homeostasis and prevent microbial invasion natural barrier; however, a large area exposed to the outside features, making it vulnerable to skin burns, mechanical injuries and other foreign harm. 由于金黄色葡萄球菌、大肠杆菌等病原体在伤口上繁殖,则容易导致伤口感染;感染的伤口不易愈合,容易造成体液流失并引起各种并发症。 Since Staphylococcus aureus, Escherichia coli and other pathogens propagated on the wound, can easily lead to wound infections; infected wound healing, easily lead to loss of body fluids and cause complications.

[0003] 称为传统敷料的纱布类敷料,一般由棉花、软麻布和亚麻布加工而成。 [0003] referred to as a conventional gauze dressings dressings, generally machined from cotton, lint and linen. 这类敷料实用性最强、影响力最大、应用时间最长,目前仍占有很大市场份额。 Such dressing the most practical, the most influential, longest application, still account for a large market share. 该类敷料优点是:有一定吸收性,制作简单,价格便宜,可重复使用;缺点是:无法保持创面湿润导致创面愈合延迟、敷料纤维易造成异物反应从而影响愈合、换药时易损伤新生的组织、病原体易通过被浸透的敷料、换药工作量大等,且纱布敷料中局部应用抗生素导致的细菌耐药更使感染创面难以愈合。 Such dressings advantages are: there is a certain absorbency, making simple, inexpensive, reusable; disadvantages are: unable to keep the wound moist wound healing results in a delay, caused by a foreign body reaction dressing fiber is easy to affect the healing, when the dressing is easy to damage the newborn tissue, pathogen easily by being impregnated dressings, and other heavy workload dressing, gauze dressing and bacterial resistance caused by topical antibiotics more difficult to heal wounds infectivity.

[0004] 1962年,英国科学家George Winter博士发明了“湿法疗法”,他在动物实验中发现,在密闭湿润环境下伤口愈合速度比暴露于空气中干燥创面更快一倍,从而奠定了采用新型敷料处理创面的理论基础。 [0004] In 1962, British scientist Dr. George Winter invented the "wet therapy", he found that in animal experiments, double the rate of wound healing faster than exposure to air drying in a confined moist wound environment, thus laying the adoption the new dressing processing theory foundation wounds. 自此,基于这一思想的各种新式敷料得到广泛的发展,如水状胶体敷料、水凝胶敷料等。 Since then, a variety of new dressing based on this idea is widely developed, such as water hydrocolloid dressing, hydrogel dressings. 水凝胶敷料具有可以明显缓解疼痛,吸收伤口渗出液,透气保湿,且更换敷料时不会破坏已愈合的伤口组织,透明可以观察伤口愈合情况等优点;同时,现已证明使用保湿敷料能给创伤局部创造一个仿效创面水泡生理愈合的湿润条件,使伤口再上皮化能力显著提高,使创面以更快的速度愈合。 The hydrogel dressing has significantly alleviated the pain, wound exudate absorption, moisture permeability, without disrupting the healed wound tissue replacement dressings, transparent wound healing can be observed, etc; Meanwhile, it has proved able to use a moisturizer dressing wet conditions create localized trauma to emulate a blister physiological wound healing, the wound re-epithelialization capacity significantly increased, so that the wounds heal faster. 因此,水凝胶敷料得到各国的普遍重视。 Therefore, the hydrogel dressing universal national attention.

[0005] 水凝胶敷料的主要制备方法有化学法、冷冻法和辐射法等。 [0005] The main method of preparing hydrogel dressings chemical method, radiation method and the like, and freezing. 化学法制备的水凝胶中通常会残余反应过程所需的交联剂和引发剂等有害小分子物质,影响其使用。 Chemical method hydrogel typically required for the reaction of residual crosslinking agent and initiator and the like harmful process small molecules, affect their use. 冷冻法是由P印pas最早报导的一种非常有效的制备聚乙烯醇水凝胶的方法,该方法制得的水凝胶具有弹性好和机械强度大等优点,但这种水凝胶是一种物理凝胶,在较高温度时会融解成溶液,并且水凝胶的溶胀度较小且不透明,影响治疗过程中对伤口的观察。 Freezing is a very effective method of preparing a polyvinyl alcohol hydrogel is first reported by P pas printing, the process to obtain a hydrogel with good mechanical strength and elasticity, etc., but is such hydrogels kind of physical gel at higher temperatures to melt into the solution, and the degree of swelling of the hydrogel is small and opaque, observation of the wound during the treatment effect. 文献中报道的冷冻融化时间通常均为8-24h,制备周期长,不利于产品的生产。 Reported in the literature are typically freeze thawing time 8-24h, the preparation cycle is long, is not conducive to production. 辐射法通过高能辐射引发交联制得水凝胶敷料,其过程不需添加任何化学交联剂和引发剂等有害物质,产品纯净,此外,辐射可在室温下进行,反应条件温和,且可以通过控制辐射剂量、剂量率等控制产品性能,同时凝胶形成与灭菌可同时进行。 Irradiation by high energy radiation crosslinking initiator to prepare a hydrogel dressing process without adding any chemical crosslinkers and initiators harmful substances, product purity, Furthermore, the radiation may be carried out at room temperature, mild reaction conditions, and may be by controlling the irradiation dose, dose rate control performance while gel formation can be performed simultaneously with the sterilization.

[0006] 公布于2007年6月13日的《含药物、壳聚糖的聚乙烯醇水凝胶敷料及其制备方法》(CN1320931C)公开了一种含药物、壳聚糖的聚乙烯醇水凝胶敷料,该医用水凝胶敷料可用于治疗各种皮肤创伤和烧烫伤,但是具有吸渗液能力差、存在增塑剂等有害物质残留的缺点;公布于2011年7月6日的《一种含纳米银和羧甲基壳聚糖医用敷料及其制备方法》(CN102114265A)公开了一种含纳米银和羧甲基壳聚糖医用敷料,具有较好的抗菌功效,但是具有无法保持创面湿润,导致创面愈合延迟;不易从创面揭起等缺点;公布于2006年10月25日的《具有促进伤口愈合、抗菌功能的液体伤口敷料》(CN100471528C)公开了一种具有促进伤口愈合、抗菌功能的液体(凝胶状)伤口敷料,该医用敷料具有抗炎止痛、促进伤口愈合的功效,但是具有吸收渗出液能力差的缺点。 [0006] published on June 13, 2007 at the "polyvinyl alcohol hydrogel and method for preparing drug-containing dressings, chitosan" (CN1320931C) discloses a drug containing a polyvinyl alcohol, chitosan hydrogel dressings, hydrogel dressings which can be used for the medical treatment of various skin wounds and burns, but with poor absorption of exudate, the presence of plasticizers and other harmful substances remaining drawbacks; published on July 6, 2011 in " containing nanosilver and carboxymethyl chitosan medical dressing and preparation method "(CN102114265A) discloses a nanosilver-containing medical dressings and carboxymethyl chitosan, having a good antimicrobial efficacy, but have not kept the wound moist, resulting in delayed wound healing; and other shortcomings easy to lift from the wound; published on October 25, 2006 of "promote wound healing, wound dressing liquid antibacterial function" (CN100471528C) discloses a wound to promote healing, antibacterial function liquid (gel) wound dressing, the medical dressing having anti-inflammatory analgesic, promote wound healing effect, but has disadvantages of poor absorption of exudate capabilities.

发明内容 SUMMARY

[0007] 本发明的目的在于提供一种能吸收渗出液、透明、透气、防水、不与组织粘连、柔韧性好,且具有抗菌性能的适用于烧烫伤、机械性伤口、手术切口及慢性创面保护和治疗的抗菌促愈水凝胶敷料。 [0007] The object of the present invention is capable of absorbing exudate to provide a transparent, air-permeable, waterproof, and tissue adhesion, good flexibility, and is suitable for burns, mechanical wounds, surgical incisions and chronic having antibacterial properties antibacterial protection and treatment of wounds to promote healing hydrogel dressing.

[0008] 为解决上述技术问题,本发明采取以下技术方案:一种抗菌促愈水凝胶敷料,其主要成分为水溶性的高分子聚合物、具有假塑性的功能聚合物、抗菌剂和水。 [0008] To solve the above problems, the present invention adopts the following technical solution: An antibacterial Cuyu hydrogel dressing, whose main component is a water-soluble polymer, functional polymer having a pseudoplastic, antibacterial and water . 各主要成分的含量为:按质量分数计2% -12%的水溶性的高分子聚合物,0.1% -4%具有假塑性的高分子聚合物,0.01% -3%的抗菌剂和余量的水。 The main content of each component is: polymer mass fraction of 2% to 12% of a water soluble, 0.1% to 4% having a pseudoplastic polymer, from 0.01% to 3%, and the balance of the antimicrobial agent of water.

[0009] 其中,所述水溶性的高分子聚合物可为天然水溶性高分子聚合物和合成水溶性高分子聚合物中的一种或几种,或者为两者的组合。 [0009] wherein the water-soluble high polymer may be one or more natural water-soluble polymers and synthetic water-soluble polymer, or a combination of both. 所述天然水溶性高分子聚合物可为海藻酸及其衍生物、透明质酸及其衍生物等中的一种或几种的组合;所述海藻酸衍生物可选自海藻酸钠、海藻酸镁、海藻酸钙和海藻酸钾等;所述透明质酸衍生物可选自透明质酸、透明质酸钠、透明质酸钙和透明质酸锌等。 The natural water-soluble polymers may be alginic acid and a combination of one or more derivatives of hyaluronic acid and derivatives thereof; the alginic acid derivative selected from sodium alginate, magnesium, calcium alginate, potassium alginate, and the like; a derivative of hyaluronic acid selected from hyaluronic acid, sodium hyaluronate, zinc hyaluronate, and calcium hyaluronate. 所述合成水溶性高分子聚合物可为聚氨酯、聚丙烯酰胺、聚乙烯醇、聚乙烯吡咯烷酮、聚氧化乙烯和聚丙烯酸钠中的一种或几种的组合;其中较优选的高分子聚合物为聚乙烯醇和聚乙烯吡咯烷酮。 The synthetic water-soluble polymers may be polyurethane, polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, polyethylene oxide, a combination of ethylene and one or more sodium polyacrylate; wherein more preferably the polymer polyvinyl alcohol and polyvinyl pyrrolidone.

[0010] 所述具有假塑性的天然高分子聚合物可为琼脂粉、琼脂糖、明胶和卡拉胶等中的一种或几种的组合。 Natural polymers [0010] The pseudoplastic agar powder may be a combination of one or more of agarose, gelatin, carrageenan, and the like.

[0011] 所述抗菌剂为能有效杀死致病菌的物质,可选自无机抗菌剂、有机抗菌剂和天然抗菌剂中的一种或几种;其中:较优选的抗菌剂为无机抗菌剂或天然抗菌剂;所述无机抗菌剂可为银离子和/或纳米银,所述银离子和/或纳米银以离子、单质的形式存在;所述有机抑菌剂可为水溶性的季铵盐类抗菌剂,如季铵盐型阳离子聚丙烯酰胺杀菌剂、单吡啶季铵盐杀菌剂、双季铵盐杀菌剂和/或复合季铵盐杀菌剂等;所述天然抗菌剂可为具有抑菌作用的天然动植物的提取物,如甲壳素、壳聚糖及其衍生物、芥末、蓖麻油和/或山葵等,优选为甲壳素、壳聚糖及其衍生物。 [0011] The antimicrobial agent is effective in killing the bacteria species selected from the one or more inorganic antibacterial agents, organic antibacterial agents, and natural antibacterial agent; wherein: more preferred antimicrobial agent is an inorganic antibacterial natural antibacterial agents or agents; the inorganic antibacterial agent may be silver ions and / or nano silver, the silver ions and / or nano silver is present as an ion, single form; the bacteriostatic agent may be water soluble organic quaternary ammonium antimicrobial agent, such as a quaternary ammonium salt type cationic polyacrylamide fungicides, bactericides mono pyridinium salt, quaternary ammonium bactericides double and / or fungicides, and other quaternary ammonium compound; the natural antimicrobial agent may be natural plant and animal extracts have antibacterial action, such as chitin, chitosan and its derivatives, mustard, castor oil and / or horseradish, preferably chitin, chitosan and derivatives thereof. 所述壳聚糖及其衍生物为小分子量壳聚糖(Mr:2万)或大分子量壳聚糖(Mr:200万)与小分子量壳聚糖(Mr:2万)合用。 The low molecular weight chitosan and chitosan derivatives (Mr: 2 million) or high molecular weight chitosan (Mr: 200 million) and low molecular weight chitosan (Mr: 2 million) combined.

[0012] 本发明另一目的在于提供一种制备上述抗菌促愈水凝胶敷料的方法。 [0012] Another object of the present invention to provide a process for preparing the above antimicrobial Cuyu hydrogel dressing.

[0013] 所述抗菌促愈水凝胶敷料的方法,包括以下步骤: [0013] Antibacterial Cuyu the hydrogel dressing method, comprising the steps of:

[0014] I)按配方称取各组分; [0014] I) according to the formulation of each component weighed;

[0015] 2)将水溶性高分子聚合物和具有假塑性的高分子聚合物溶于水中,在90°C以上水浴中回流形成均匀溶液A,再将抗菌剂溶于水中,搅拌形成均一溶液B,最后将A、B两种溶液混合,用水补足至总重量,搅拌均匀成均一溶液; [0015] 2) a water-soluble polymer and a polymer having a pseudoplastic dissolved in water to form a homogeneous solution A was refluxed at 90 ° C above a water bath, and then the antimicrobial agent is dissolved in water and stirred to form a homogeneous solution B, and finally the A, B two solutions were mixed, made up with water to a total weight, stir into a homogeneous solution;

[0016] 3)将步骤2)中的均一溶液进行消泡处理后,倒入成形模具中,冷却形成假塑性固体;[0017] 4)对步骤3)中的假塑性固体在室温下进行辐照交联和灭菌,辐照方式可为60Co Y-射线或高能电子束射线,辐照时间为10-40min,辐照剂量为10K-70K。 After [0016] 3) the homogenous solution of step 2) is subjected to defoaming treatment, is poured into the mold, cooled to form a solid pseudoplastic; [0017] 4) in Step 3) solid is pseudoplastic radiation at room temperature according crosslinking and sterilization, irradiated manner as 60Co Y- rays or high energy electron beam radiation, the irradiation time is 10-40min, exposure dose 10K-70K.

[0018] 其中,所述步骤3)中的消泡处理方式为静置消泡、超声消泡或真空除泡。 [0018] wherein the defoaming treatment) in the step 3 was allowed to stand for defoaming, ultrasonic defoaming or vacuum defoaming.

[0019] 本发明以上述方案,提供了一种能吸收渗出液,透明、透气、防水、不与组织粘连、柔韧性好,且具有抗菌性能的,适用于烧烫伤、机械性伤口、手术切口及慢性创面保护和治疗的抗菌促愈水凝胶敷料及其制备方法。 [0019] In the above-described embodiment of the present invention, there is provided a can absorb exudate, transparent, air-permeable, waterproof, and tissue adhesion, good flexibility, and have antibacterial properties, suitable for burns, mechanical wounds, surgery hydrogel dressings antibacterial incision and its preparation method of protecting and treating chronic wound Cuyu. 该敷料主要成分为水溶性的高分子聚合物、具有假塑性的功能聚合物、抗菌剂和水,经一系列工艺将其溶为一体,溶液浇注在模具中,固化后经辐照灭菌获得。 The dressing of the main components of a water-soluble polymer, functional polymer having a pseudoplastic, antibacterial and water, through which a series of processes as one solution, a solution cast in the mold, obtained after curing by irradiation sterilization . 创面愈合试验、体外抗菌试验测试结果显示本发明能够有效促进创面愈合,并对多种细菌具有较好的抑菌活性,抑菌率达70%以上。 Experimental wound healing, in vitro antibacterial test results show the present invention can effectively promote wound healing, and more bacteria have good antibacterial activity, inhibitory rate of 70%. 本发明的积极进步之处在于: Actively progress of the present invention:

[0020] (I)该水凝胶敷料通过辐照方式交联与灭菌同步完成,制备方法简单,大大缩短了制备周期,且产物纯净,所得的凝胶透明度高、溶胀度大、强度高。 [0020] (I) hydrogel dressing crosslinked by irradiation sterilization way synchronization is complete preparation method is simple, greatly reducing the preparation period, and the product purity, high transparency of the resulting gel, a large degree of swelling, high strength .

[0021] (2)本发明选取琼脂粉、琼脂糖、明胶、卡拉胶等具有假塑性的天然高分子聚合物中的一种或几种,使样品在室温下预固化,形成假性凝胶,利于辐照过程之前的包装与运输。 [0021] (2) Select the present invention, powder agar, agarose, gelatin, carrageenan and other natural polymers having a pseudoplastic of one or more, the sample was pre-cured at room temperature to form a pseudo-gel , packaging and transport of favor before the irradiation process.

[0022] (3)本发明中的水凝胶中可添加无机抗菌剂或天然抗菌剂中的一种或几种,可以有效地抑制创面的细菌感染,对革兰氏阳性菌和阴性菌具有很好的抑制作用,不易产生耐药性,促进细胞再生,加速创面修复。 [0022] (3) in the present invention may be added to the hydrogel or natural inorganic antibacterial agent in antibacterial agent is one or more, can be effectively suppressed bacterial wound infections, having Gram-positive and Gram-negative bacteria good inhibition, easy to produce drug resistance, promote cell regeneration, accelerate wound healing.

[0023] (4)本发明中的水凝胶不含其它具有刺激性的溶剂及保湿剂、增塑剂等添加剂,避免了该类物质对皮肤的毒副作用,提高了其生物相容性。 [0023] (4) the hydrogel of the present invention is free of other solvents and irritating humectant, with additives such as plasticizer, avoiding the toxic side effects of such substances on the skin, improving its biocompatibility.

[0024] 综上所述,本发明的抗菌促愈水凝胶敷料明显优于现有的水凝胶敷料,具有抗菌疗效显著、透明、含水量高、制备方法简单、产物纯净、无毒无害、无刺激、生物相容性好等优点,适合大面积推广和应用。 [0024] In summary, the present invention is the antimicrobial hydrogel dressings Cuyu significantly better than conventional hydrogel dressings having significant antibacterial effect, transparent, high water content, preparation method is simple, pure product, non-toxic harm, no stimulation, good biocompatibility, etc., suitable for large-scale promotion and application.

[0025] 下面结合具体实施例对本发明做进一步详细说明。 [0025] Specific embodiments of the present invention in conjunction with the following described in further detail.

具体实施方式 detailed description

[0026] 本发明提供了一种抗菌促愈水凝胶敷料,其主要成分为水溶性的高分子聚合物、具有假塑性的功能聚合物、抗菌剂和水。 [0026] The present invention provides an antimicrobial Cuyu functional polymer hydrogel dressing, whose main component is a water-soluble polymer, having a pseudoplastic, antibacterial and water.

[0027] 具体来讲,所述抗菌促愈水凝胶敷料各主要成分的含量为:按质量分数计2% -12%的水溶性高分子聚合物,0.1% -4%具有假塑性的高分子聚合物,0.01% -3%的抗菌剂和余量的水。 [0027] Specifically, the antimicrobial Cuyu major component content of the hydrogel dressing: mass fraction of 2% to 12% of a water soluble polymer, 0.1% to 4% with high pseudoplasticity polymer molecule, from 0.01% to 3% of antimicrobial agent and the balance water.

[0028] 水溶性的高分子聚合物 [0028] The water-soluble polymers

[0029] 本发明中,水溶性的高分子聚合物可为天然和合成的高分子聚合物中的一种或几种,或者为两者的组合,组合时比例没有限定。 [0029] In the present invention, the water-soluble polymer may be one or more of natural and synthetic polymers in the, or a combination of both, when the combination ratio is not particularly limited.

[0030] 天然水溶性高分子聚合物可为海藻酸及其衍生物、透明质酸及其衍生物等中的一种或几种的组合,组合时比例没有限定;所述海藻酸衍生物可选自海藻酸钠、海藻酸镁、海藻酸钙和海藻酸钾等;所述透明质酸及其衍生物可选自透明质酸、透明质酸钠、透明质酸钙和透明质酸锌等。 [0030] The natural water-soluble polymers may be a combination of one or more of alginic acid and its derivatives, hyaluronic acid and derivatives thereof, when the combination ratio is not particularly limited; the alginic acid derivative may be is selected from sodium alginate, alginic acid, magnesium, calcium alginate, potassium alginate, and the like; the hyaluronic acid and derivatives selected from hyaluronic acid, sodium hyaluronate, calcium hyaluronate and hyaluronic acid zinc . 天然水溶性高分子聚合物具有独特的生物相容性和生物活性,可作为水凝胶敷料的有效成分。 Natural water-soluble polymers with unique biocompatibility and bioactivity, as an active ingredient can be a hydrogel dressing. 辐射法制备医用水凝胶敷料时,添加一定比例的天然水溶性高分子聚合物,可以提高水凝胶的生物相容性:同时,水溶性天然高分子聚合物在射线作用下降解后可以填充在凝胶网络空隙中,起到提高凝胶强度的作用。 When the irradiation method Preparation of Medical hydrogel dressings, certain proportion of the natural water-soluble polymers, biocompatible hydrogel can be improved: the same time, decrease the water-soluble natural polymers in solution may be filled after the ray, voids in the gel network, serve to increase the gel strength.

[0031] 合成水溶性高分子聚合物可为聚氨酯、聚丙烯酰胺、聚乙烯醇、聚乙烯吡咯烷酮、聚氧化乙烯和聚丙烯酸钠中的一种或几种的组合,组合时比例没有限定,其中较优选的为聚乙烯醇和聚乙烯吡咯烷酮。 [0031] The synthetic water-soluble polymers may be polyurethane, polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, polyethylene oxide, a combination of ethylene and one or more sodium polyacrylate, the combination ratio is not defined, wherein more preferably polyvinyl alcohol and polyvinyl pyrrolidone. 合成的水溶性高分子聚合物因其良好的生物相容性、机械性能及安全性,而广泛应用于医疗事业中。 Synthetic water-soluble polymers because of its good biocompatibility, mechanical properties and safety, is widely used in the medical industry. 如由其制造而成的水凝胶在眼科、伤口敷料和人工关节方面的有广泛应用,具有独特的强力粘接性、柔韧性、平滑性、耐油性、耐溶剂性、保护胶体性、耐磨性以及经特殊处理具有耐水性等优点。 The manufactured therefrom hydrogel in ophthalmology, wound dressings, artificial joints, and has wide application areas, with a unique strong adhesion, flexibility, smoothness, oil resistance, solvent resistance, protective colloid resistance, grindability and treated with special advantages such as water resistance.

[0032]具有假塑性的天然高分子聚合物[0033] 本发明中,具有假塑性的天然高分子聚合物可为琼脂粉、琼脂糖、明胶和卡拉胶等中的一种或几种的组合,组合时比例没有限定。 Natural polymers [0032] Natural polymers having pseudoplastic [0033] In the present invention, having a pseudoplastic can be combined into one or more of agar, agarose, gelatin, carrageenan, and the like when the combination ratio is not defined. 该类聚合物具有可溶于热水中形成溶液,而降温后则形成假性固体的性质,对样品起到预固形的作用,便于进行下一步的操作。 Such polymers may be dissolved in hot water with a solution, and after cooling the solid is formed pseudo properties, a solid sample pre-play the role of facilitating the next operation.

[0034] 抗菌剂 [0034] The antibacterial agent

[0035] 本发明中,抗菌剂为能有效杀死致病菌的物质,可选自无机抗菌剂、有机抗菌剂和天然抗菌剂中的一种或几种,组合时比例没有限定,其中较优选的抗菌剂为无机抗菌剂或天然抗菌剂。 [0035] In the present invention, the antimicrobial agent is effective in killing the bacteria species selected from the inorganic antibacterial agent, one or more natural antibacterial agents and organic antibacterial agent, when the combination ratio is not particularly limited, in which more the preferred antimicrobial agent is an inorganic antibacterial or natural antibacterial agent.

[0036] 无机抗菌剂可为银离子和/或纳米银,所述银离子和/或纳米银以离子、单质的形式存在; [0036] Inorganic antibacterial agent may be silver ions and / or nano silver, the silver ions and / or nano silver ions present in elemental form is;

[0037] 有机抑菌剂可为水溶性的季铵盐类抗菌剂,如季铵盐型阳离子聚丙烯酰胺杀菌齐ϋ、单吡啶季铵盐杀菌剂、双季铵盐杀菌剂和/或复合季铵盐杀菌剂等; [0037] The organic antimicrobial agent may be water soluble quaternary ammonium antimicrobial agent, such as a quaternary ammonium salt type cationic polyacrylamide sterilizing homogeneous ϋ, mono pyridinium salt fungicides, bactericides bis quaternary ammonium salt and / or complex quaternary ammonium fungicides;

[0038] 天然抗菌剂可为具有抑菌作用的天然动植物的提取物,如甲壳素、壳聚糖及其衍生物、芥末、蓖麻油和/或山葵等,优选为甲壳素、壳聚糖及其衍生物。 [0038] The natural antimicrobial agent may be a natural plant and animal extracts have antibacterial action, such as chitin, chitosan and its derivatives, mustard, castor oil and / or horseradish, preferably chitin, chitosan and derivatives thereof. 其中: among them:

[0039] 甲壳素是由N-乙酸基_2_氨基-2-脱氧-D-匍萄糖以B_l,4糖苷键形式连接而成的多糖,壳聚糖是甲壳素的脱乙酰基的产物。 [0039] Chitin is a _2_ N- acetoxy-2-deoxy -D- B_L creeping grape sugars, polysaccharides 4 linked together form glycosidic bonds, chitosan is deacetylated chitin product . 甲壳素或壳聚糖尤其是小分子量壳聚糖具有显著的抑菌作用,对于一般人体表皮存在的皮肤细菌如表皮葡萄球菌、造成烧伤病人感染的绿脓杆菌、金黄色葡萄球菌和酿脓链球菌等均有明显的抑制效果。 Chitin or chitosan, especially low molecular weight chitosan has a significant inhibitory effect, typically for the presence of skin bacteria such as Staphylococcus epidermidis human skin, causing burns patients infected with Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes chain bacteria, which had significant inhibitory effect. 同时,壳聚糖可以促进上皮细胞的再生,加快创口愈合的速度,提高创口愈合质量。 Meanwhile, the chitosan can promote the regeneration of epithelial cells, to speed up wound healing, to improve wound healing quality. 大分子量壳聚糖可以生物降解,产生低聚糖,寡糖,甚至单糖,进而发挥其加速细胞增殖和加强组织重塑的功能。 Large molecular weight chitosan are biodegradable, producing oligosaccharides, oligosaccharides, monosaccharides and even, then play which accelerates proliferation and tissue remodeling strengthening function. 有意义的是:大分子量壳聚糖具有成膜强度好,小分子量壳聚糖具有生物活性高,保水性好的特点,本发明把大分子量壳聚糖和小分子量壳聚糖合用可以充分发挥各自的优点,制备性能优良的医用敷料。 Interest are: high molecular weight chitosan having a deposition strength, low molecular weight chitosan having a high biological activity, and good water retention, according to the present invention, the low molecular weight chitosan and high molecular weight chitosan in combination can give full play respective advantages, to prepare better medical dressing.

[0040] 本发明中,所述水为纯化水(洁净的去离子水或蒸馏水)。 [0040] In the present invention, the water is purified water (clean deionized or distilled water).

[0041] 本发明的另一个目的是提供一种制备上述抗菌促愈水凝胶敷料的方法,具体可包括以下步骤: [0041] Another object of the present invention is to provide a process for preparing the above antimicrobial Cuyu hydrogel dressing, may specifically include the following steps:

[0042] I)按配方称取各组分; [0042] I) according to the formulation of each component weighed;

[0043] 2)将水溶性高分子聚合物和具有假塑性的高分子聚合物溶于水中,在90°C以上水浴中回流形成均匀溶液A,再将抗菌剂溶于水中,搅拌形成均一溶液B,最后将A、B两种溶液混合,用水补足至总重量,搅拌均匀成均一溶液; [0043] 2) a water-soluble polymer and a polymer having a pseudoplastic dissolved in water to form a homogeneous solution A was refluxed at 90 ° C above a water bath, and then the antimicrobial agent is dissolved in water and stirred to form a homogeneous solution B, and finally the A, B two solutions were mixed, made up with water to a total weight, stir into a homogeneous solution;

[0044] 3)将步骤2)中的均一溶液进行消泡处理后,倒入成形模具中,冷却形成假塑性固体; [0044] 3) in step 2) is a homogeneous solution after defoaming treatment, is poured into the mold, cooled to form a solid pseudoplastic;

[0045] 4)对步骤3)中的假塑性固体在室温下进行辐照交联和灭菌,辐照方式可为6tlCo Y-射线或高能电子束射线,辐照时间为10-40min,辐照剂量为10K-70K。 [0045] 4) in Step 3) pseudoplastic solid was radiation cross-linking and sterilization, irradiation mode may be 6tlCo Y- rays or high energy electron beam radiation, the irradiation time was 10-40min at room temperature, radiation dose for the 10K-70K.

[0046] 在上述制备方法中,所述步骤3)中的消泡处理方式可为静置消泡、超声消泡或真空除泡。 [0046] In the above production method, the defoaming treatment) in step 3 may be allowed to stand for defoaming, ultrasonic defoaming or vacuum defoaming.

[0047] 实施例在以本发明技术方案为前提下进行实施,给出了详细的实施方式和具体的操作过程,实施例将有助于理解本发明,但是本发明的保护范围不限于下述的实施例。 [0047] In embodiments of the present invention is a technical premise, gives a detailed and specific embodiments of operation, it will be helpful in understanding embodiments of the present invention, but the scope of the present invention is not limited to the following embodiment.

[0048] 下述实施例中所用方法如无特别说明均为常规方法。 [0048] Example methods used in the following embodiments unless otherwise specified are typical methods.

[0049] 实施例1-10、制备抗菌促愈水凝胶敷料 [0049] Examples 1-10 were prepared antimicrobial hydrogel dressings Cuyu

[0050] 本发明实施例1-10抗菌促愈水凝胶敷料及比较例的配方如表1-2所示[0051 ] 表1实施例1-5抗菌促愈水凝胶敷料及比较例I的配方 [0050] Formulation Examples 1-10 embodiment of the present invention, the antimicrobial hydrogel dressings Cuyu and Comparative Examples as shown in Table 1-2 [0051] Table 1-5 Example 1 Antibacterial Cuyu Hydrogel dressings and Comparative Example I recipe

[0052](每1000g抗菌促愈水凝胶中各组分的克数) [0052] (grams of each component per 1000g Cuyu antimicrobial hydrogel)

[0053] [0053]

Figure CN103520767AD00071

[0054] 表2实施例6-10抗菌促愈水凝胶敷料及比较例2的配方 [0054] TABLE 2 Formulation Examples 6-10 and promote healing antimicrobial hydrogel dressings and Comparative Example 2

[0055](每1000g抗菌促愈水凝胶中各组分的克数) [0055] (grams of each component per 1000g Cuyu antimicrobial hydrogel)

[0056] [0056]

Figure CN103520767AD00081

[0058] 试验一、产品性能参数检测 [0058] a test, the detection performance parameters

[0059] 对实施例1-10制备的水凝胶敷料的性能参数进行检测,实施例1-10的产品符合以下要求: [0059] The performance parameters of the hydrogel prepared in Examples 1-10 dressing embodiment is detected, the product in Example 1-10 meet the following requirements:

[0060] 1、性状:为淡黄色或黄色透明凝胶。 [0060] 1. Properties: pale yellow or yellow transparent gel.

[0061] 2、吸水率:取实施例1-10的样品各少许,准备称重(Wtl),在纯化水中浸泡24h后,取出用滤纸擦干表面水分,准确称重(Wt),水凝胶增重的部分(Wt-Wtl)占水凝胶原始重量(Wtl)的百分比,即吸水率。 [0061] 2, water absorption: Take a small sample of each of Examples 1-10, Preparation weighed (Wtl), after 24h immersion in purified water, surface water removed with a dry filter paper, accurately weighed (Wt), the hydraulic the percentage weight gain of the adhesive portion (wt-Wtl) representing the original hydrogel weight (Wtl), i.e. water absorption. 实施例1-10的样品吸水率均大于200%。 Example 1-10 Samples of water absorption greater than 200%.

[0062] 3、产品本身细菌测定:方法按GB/T14233.2-2005有关规定进行。 [0062] 3, the product itself bacterial assay: method according to GB / T14233.2-2005 provisions. 测定结果表明产品本身无菌。 Results showed that the product itself is sterile.

[0063] 4、皮肤刺激指数(PU)测定:按GB/T16886.10-2005规定的方法进行,要求急性接触24小时,指数均不大于0.5。 [0063] 4, index (PU) Skin irritation was measured: for predetermined GB / T16886.10-2005 method, requires 24 hours of acute exposure, index not greater than 0.5. 测定结果表明产品符合要求。 Results showed that the product meets the requirements.

[0064] 5、皮肤致敏反应测定:按GB/T16886.10-2005规定的封闭式致敏试验方法进行,均无皮肤致敏反应。 [0064] 5. Determination of skin sensitization reactions: Closed sensitization test methods according to GB / T16886.10-2005 be predetermined, no skin sensitization. 测定结果表明产品不引起皮肤致敏反应。 Results showed that the product does not cause skin sensitization.

[0065] 试验二、创面愈合试验 [0065] The second experiment, wound healing test

[0066] 为了验证本发明水凝胶敷料的使用有效性,选取实施例、比较例与上市样品进行创面愈合对照试验。 [0066] In order to verify the effectiveness of the use of the hydrogel dressing of the present invention, selected Examples and Comparative Examples listed samples with controlled trial wound healing. 具体试验方法如下: Specific test methods are as follows:

[0067] 试验动物选用1.8-2.5Kg的新西兰白兔,观察点分别为3d、5d、10d、15d,每次相点13只动物。 [0067] Experimental animals New Zealand White rabbits 1.8-2.5Kg selected observation points are 3d, 5d, 10d, 15d, each phase point 13 animals. 白兔背部于试验前24h-72h背部剪毛后,用脱毛膏做脱毛处理。 Rabbits after 24h-72h back to back before shearing test, depilatory creams made with depilation. 用2.0%的戊巴比妥钠,按lmL/Kg耳缘静脉将白兔麻醉,用80°C热水烫伤10s,在白兔背部致为I度至浅II度烫伤,左右两侧各2处,间隔2cm以上。 With 2.0% sodium pentobarbital, press lmL / Kg ear vein to anesthetized rabbits, scalded with hot water 80 ° C 10s, actuation of I to II degree superficial burns at the back of rabbits, right and left sides 2 at least 2cm intervals. 烫伤Id后分别将实施例1-实施例10所得的抗菌促愈水凝胶敷料在每只白兔背部四个烫伤部位作为实验组1-实验组10 ;将比较例I和比较例2所得的抗菌促愈水凝胶敷料作为对照组I和对照组2 ;将上市样品冷宁康(国内一种类似产品)覆盖创面,作为对照组3 ;干纱布作为对照组4,于各取样时间点计算创面的愈合率。 Id scalded Example 1 respectively Example 10 Cuyu resulting antimicrobial hydrogel dressing in the back of four rabbits per experimental group burn site 10 1- experimental group; Comparative Example 2 Comparative Example I and the resulting prohealing antimicrobial hydrogel dressing as control group I and group 2; hydrogel samples will be available (a similar domestic product) covering the wound, as the control group 3; 4 dry gauze as a control group was calculated at each sampling time point wound healing rate.

[0068] 创面愈合率的计算方法如下:先将创面描记在半透明纸上,再以此为模板,将质地均匀的硬纸片剪成同样大小,然后用天平称量,以硬纸片的质量间接地表示创面面积的大小。 [0068] The method of calculating the rate of wound healing are as follows: firstly wound tracings translucent paper, and then as a template, a uniform texture cardboard cut the same size, and then weighed with a balance, to the cardboard mass indirectly represent the size of the wound area. 按下式计算创面愈合率:创面愈合率(% )=(原始创面面积-未愈合创面面积)/原始创面面积。 Wound healing rate is calculated as follows: healing ratio (%) = (original wound area - an area of ​​non-healing wounds) / original wound area. 观察创面愈合情况,根据创面愈合情况确定愈合时间。 Of wound healing, the healing of the wound is determined in accordance with healing time.

[0069] 试验结果如表3所示,从上表中创面平均愈合率的结果得出:试验组1-10对创面愈合的积极效果优于对照组1-3,与对照组4比较,则可以看出本发明的水凝胶敷料比纱布对创面具有更积极的促愈合效果。 [0069] The test results are shown in Table 3, the results from the table above average healing rates obtained: Test Group 1-10 positive effect on wound healing 1-3 than the control group, compared with the control group 4, the the hydrogel dressing can be seen that the present invention has a more positive effect in wound healing promotion than gauze.

[0070] 表3创面愈合试验结果 [0070] Table 3 Test results of wound healing

[0071] [0071]

Figure CN103520767AD00091

[0072] 试验三、产品抑菌试验 [0072] Test Third, the product Antibacterial

[0073] 参照GB/T15979-2002—次性使用卫生用品卫生标准附录C产品杀菌性能、抑菌性能及稳定性测试方法中的规定,对实施例1-10、比较例1-2制备的水凝胶敷料及上市样品冷宁康(阴性对照)进行了体外抑菌试验。 [0073] Referring to GB / T15979-2002- time use sanitary products specified in Appendix C Product Standard bactericidal properties, antibacterial properties and stability of the test method, 1-10, 1-2 prepared in water Example Comparative Example listed hydrogel dressing and a sample of hydrogel (negative control) performed in vitro antibacterial test. 试验方法为:制备浓度为104-105cfu/g的菌悬液(金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌)。 Test method: prepared at a concentration of 104-105cfu / g of bacterial suspension (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans). 取0.1mL的菌悬液均匀涂布在被试样片(2.0cmX 3.0cm)和对照样片(2.0cmX 3.0cm)上作用20min后,将样片投入PBS溶液中,充分混匀,做适当稀释,2-3个稀释度。 Take 0.1mL bacterial suspension was evenly applied after 20min acts on the specimen (2.0cmX 3.0cm) and control samples (2.0cmX 3.0cm), the swatches were put into the PBS solution, mixed well, to make the appropriate dilution, 2-3 dilution. 取0.5mL置于无菌平皿中,用营养琼脂培养基(金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌)或改良马丁琼脂培养基(白色念珠菌)30mL作倾注,转动平皿,充分混匀,置37°C ±2°C温箱(金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌)培养24h或25°C ±2°C温箱(白色念珠菌)培养72h,观察结果。 Take 0.5mL placed in a sterile petri dish, using nutrient agar (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) or modified Martin agar medium (Candida albicans) for pouring 30 mL, rotated dish and mixed well uniform, opposing 37 ° C ± 2 ° C incubator (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) cultured for 24h or 25 ° C ± 2 ° C incubator (Candida albicans) were cultured 72h, observation. 当抑菌率≥50%-90%,产品有抑菌作用,抑菌率≥ 90 %,产品有较强抑菌作用。 When the inhibition rate ≥50% -90%, the product has antibacterial effect, inhibition rate ≥ 90%, the product has a strong inhibitory effect.

[0074] 试验结果如表4所示,可以看出,本发明实施例1-10的水凝胶敷料具有较好的抑菌活性,优于比较例I (不添加具有假塑性的天然高分子聚合物的对照组)和阴性对照(t匕较例2-不添加具有假塑性的天然高分子聚合物和抗菌剂的对照组及冷宁康)。 [0074] The test results are shown in Table 4, it can be seen, hydrogel dressings of Examples 1-10 of the present invention has good antibacterial activities superior to Comparative Example I (without the addition of natural polymer having pseudoplastic the control group of the polymer) and a negative control (t dagger than Example 2 and the control group without addition of Hydrogel natural polymer and an antimicrobial agent having pseudoplastic).

[0075] 表4抑菌试验结果 [0075] The test results in Table 4 Antibacterial

Figure CN103520767AD00101

[0077] 另外,需要注意的是,上文所列的实施例及比较例仅是本发明的一部分,但本发明并不局限于此,还可以进行更多的变形。 [0077] Further, it is noted that the Examples and Comparative Examples listed above, only a part of the present invention, but the present invention is not limited thereto, can also be further modified. 因此,本领域的普通技术人员能从本发明公开部分直接导出或联想的其它可用变形,均视为本发明的保护内容。 Accordingly, those of ordinary skill in the art from the disclosure of the present invention is derived directly or part of other available association deformation, are considered the protection of the present invention.

Claims (10)

  1. 1.一种抗菌促愈水凝胶敷料,其主要成分为水溶性的高分子聚合物、具有假塑性的功能聚合物、抗菌剂和水。 1. An antimicrobial hydrogel dressings Cuyu, the main component of a water-soluble polymer, functional polymer having a pseudoplastic, antibacterial and water.
  2. 2.根据权利要求1所述的抗菌促愈水凝胶敷料,其特征在于:各主要成分的含量为:按质量分数计2% -12%的水溶性的高分子聚合物,0.1% -4%具有假塑性的高分子聚合物,0.01% -3%的抗菌剂和余量的水。 The antimicrobial according to claim 1 Cuyu hydrogel dressing, characterized in that: the content of the major component is: mass fraction of 2% to 12% of a water-soluble polymer, 0.1% -4 % pseudoplastic polymer having 0.01% to 3% of antimicrobial agent and the balance water.
  3. 3.根据权利要求1或2所述的抗菌促愈水凝胶敷料,其特征在于:所述水溶性的高分子聚合物可为天然水溶性高分子聚合物和合成水溶性高分子聚合物中的一种或几种,或者为两者的组合。 The antimicrobial of claim 1 or claim 2 Cuyu hydrogel dressing, characterized in that: said water-soluble polymer may be a natural water-soluble polymers and synthetic water-soluble polymers one or several, or a combination of both.
  4. 4.根据权利要求3所述的抗菌促愈水凝胶敷料,其特征在于:所述天然水溶性高分子聚合物可为海藻酸及其衍生物、透明质酸及其衍生物等中的一种或几种的组合;所述海藻酸衍生物可选自海藻酸钠、海藻酸镁、海藻酸钙和海藻酸钾等;所述透明质酸衍生物可选自透明质酸、透明质酸钠、透明质酸钙和透明质酸锌等。 According to claim 3, wherein said antimicrobial Cuyu hydrogel dressing, characterized in that: the natural water-soluble polymers may be alginic acid and the derivatives thereof, hyaluronic acid and a derivative or a combination of several of; the alginic acid derivative selected from sodium alginate, alginic acid, magnesium, calcium alginate, potassium alginate, and the like; a derivative of hyaluronic acid selected from hyaluronic acid, hyaluronic acid sodium hyaluronate, calcium hyaluronate and zinc.
  5. 5.根据权利要求3所述的抗菌促愈水凝胶敷料,其特征在于:所述合成水溶性高分子聚合物可为聚氨酯、聚丙烯酰胺、聚乙烯醇、聚乙烯吡咯烷酮、聚氧化乙烯和聚丙烯酸钠中的一种或几种的组合;其中较优选的高分子聚合物为聚乙烯醇和聚乙烯吡咯烷酮。 According to claim 3 Cuyu antimicrobial hydrogel dressing as claimed in claim, wherein: said synthetic water-soluble polymers may be polyurethane, polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, polyethylene oxide and sodium polyacrylate in combination one or more; and wherein more preferred polymer is polyvinyl alcohol and polyvinylpyrrolidone.
  6. 6.根据权利要求1或2或3或4或5所述的抗菌促愈水凝胶敷料,其特征在于:所述具有假塑性的天然高分子聚合物可为琼脂粉、琼脂糖、明胶和卡拉胶等中的一种或几种的组合。 The antimicrobial 1 or 2 or 3 or 4 or claim 5 Cuyu hydrogel dressing, wherein: said natural polymers having pseudoplastic may be agar, agarose, gelatin and a combination of one or more of carrageenan, and the like.
  7. 7.根据权利要求1至6任一所述的抗菌促愈水凝胶敷料,其特征在于:所述抗菌剂为能有效杀死致病菌的物质,可选自无机抗菌剂、有机抗菌剂和天然抗菌剂中的一种或几种;其中:较优选的抗菌剂为无机抗菌剂或天然抗菌剂;所述无机抗菌剂可为银离子和/或纳米银,所述银离子和/或纳米银以离子、单质的形式存在;所述有机抑菌剂可为水溶性的季铵盐类抗菌剂,如季铵盐型阳离子聚丙烯酰胺杀菌剂、单吡啶季铵盐杀菌剂、双季铵盐杀菌剂和/或复合季铵盐杀菌剂等;所述天然抗菌剂可为具有抑菌作用的天然动植物的提取物,如甲壳素、壳聚糖及其衍生物、芥末、蓖麻油和/或山葵等,优选为甲壳素、壳聚糖及其衍生物。 The antimicrobial according to any one of claims 1 to 6, Cuyu hydrogel dressing, characterized in that: the antimicrobial agent is a substance effective to kill the bacteria, may be selected from inorganic antibacterial agents, organic antibacterial agent natural antibacterial agents and one or more of; wherein: more preferred antimicrobial agent is an inorganic antibacterial or natural antibacterial agent; the inorganic antibacterial agent may be silver ions and / or nano silver, the silver ions and / or in the presence of silver nano-ions, single form; the bacteriostatic agent may be water soluble organic quaternary ammonium antimicrobial agent, such as a quaternary ammonium salt type cationic polyacrylamide fungicides, bactericides mono pyridinium salt, diquaternary ammonium bactericides and / or fungicides, and other quaternary ammonium compound; the natural antimicrobial agent may be a natural plant and animal extracts have antibacterial action, such as chitin, chitosan and its derivatives, mustard, castor oil other and / or horseradish, preferably chitin, chitosan and derivatives thereof.
  8. 8.根据权利要求7所述的抗菌促愈水凝胶敷料,其特征在于:所述壳聚糖及其衍生物为小分子量壳聚糖(Mr:2万)或大分子量壳聚糖(Mr:200万)与小分子量壳聚糖(Mr:2万)合用。 The antimicrobial according to claim 7 Cuyu hydrogel dressing, characterized in that: the chitosan and derivatives of chitosan of low molecular weight (Mr: 2 million) or high molecular weight chitosan (Mr : 200 million) and low molecular weight chitosan (Mr: 2 million) combined.
  9. 9.一种制备权利要求1-8任一项所述抗菌促愈水凝胶敷料的方法,包括以下步骤: 1)按配方称取各组分; 2)将水溶性高分子聚合物和具有假塑性的高分子聚合物溶于水中,在90°C以上水浴中回流形成均匀溶液A,再将抗菌剂溶于水中,搅拌形成均一溶液B,最后将A、B两种溶液混合,用水补足至总重量,搅拌均匀成均一溶液; 3)将步骤2)中的均一溶液进行消泡处理后,倒入成形模具中,冷却形成假塑性固体; 4)对步骤3)中的假塑性固体在室温下进行辐照交联和灭菌,辐照方式可为6tlCo Y -射线或高能电子束射线,辐照时间为10-40min,辐照剂量为10K-70K。 9. A process for preparing any of claims 1-8 a method of promoting healing antimicrobial hydrogel dressing, comprising the following steps: 1) Weigh the components according to the formulation; 2) water-soluble polymers having pseudoplastic polymers dissolved in water to form a homogeneous solution a was refluxed at 90 ° C above a water bath, and then the antimicrobial agent is dissolved in water and stirred to form a homogeneous solution B, the last a, B two solutions are mixed, supplemented with water to the total weight, stir into a homogeneous solution; 3) in step 2) in a homogeneous solution was defoamed, poured into the mold, cooled to form a solid pseudoplastic; 4) step) of solid 3 pseudoplasticity irradiation crosslinking and sterilization, irradiation mode may be at room temperature 6tlCo Y - rays, or high-energy electron beam radiation, the irradiation time is 10-40min, exposure dose 10K-70K.
  10. 10.根据权利要求9所述的制备方法,其特征在于:所述步骤3)中的消泡处理方式为静置消泡、超声消泡或真空除泡。 10. The production method according to claim 9, wherein: said defoaming treatment in step 3) was allowed to stand for defoaming, ultrasonic defoaming or vacuum defoaming.
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103769062A (en) * 2014-02-27 2014-05-07 江南大学 Functionalized polyvinyl alcohol hydrogel and preparation method thereof
CN103893157A (en) * 2014-04-09 2014-07-02 山东赛克赛斯药业科技有限公司 Hydrogel eye protective pad dressing and preparation method thereof
CN104027837A (en) * 2014-06-20 2014-09-10 东华大学 Preparation method of sodium alginate-calcium carbonate hybrid particle modified functional cotton fabric
CN104307031A (en) * 2014-11-10 2015-01-28 刘维峰 Preparation method and usage of external use skin repair material
CN104474583A (en) * 2014-11-18 2015-04-01 长春吉原生物科技有限公司 Flake hydrogel material and preparation method thereof
CN105088758A (en) * 2014-05-05 2015-11-25 江苏聚威新材料有限公司 Finishing method for interweaved fabric
CN105239251A (en) * 2015-10-07 2016-01-13 俞松炜 Antisepsis fabric
CN105327386A (en) * 2014-08-06 2016-02-17 上海威尔医疗保健厂 Functional hydrogel medical dressing
CN105327385A (en) * 2014-08-06 2016-02-17 上海威尔医疗保健厂 Preparation method of functional hydrogel medical dressing
CN105440297A (en) * 2015-04-21 2016-03-30 湖南工业大学 High-impact chitin composite gel preparation method
CN106117569A (en) * 2016-06-24 2016-11-16 青岛中腾生物技术有限公司 Marine furcellaran aquagel and preparation method therefor
CN106346018A (en) * 2016-09-21 2017-01-25 武汉工程大学 Preparation method and application of agarose/nano-silver composite gel

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6706279B1 (en) * 2000-10-17 2004-03-16 Pharma Mag Inc. Wound dressing
CN1579559A (en) * 2004-05-14 2005-02-16 中国科学院长春应用化学研究所 Dressing material containing medicine chitoholosida and its preparation method
CN1850291A (en) * 2006-06-09 2006-10-25 山东省医疗器械研究所 Liquid wound-dressing with functions of promoting heal of wound and bacterial-resisting
CN1944495A (en) * 2006-09-29 2007-04-11 北京大学 Water gel containing natural high molecule and its radiation preparing method
KR20120035032A (en) * 2010-10-04 2012-04-13 한국원자력연구원 Hydrogels for wound dressing comprising nano-silver particle and preparation method thereof
CN102698313A (en) * 2012-01-11 2012-10-03 北京大学 Nano-silver antibacterial hydrogel and preparation method thereof
CN103316033A (en) * 2013-07-03 2013-09-25 康晓飞 Gel and use thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6706279B1 (en) * 2000-10-17 2004-03-16 Pharma Mag Inc. Wound dressing
CN1579559A (en) * 2004-05-14 2005-02-16 中国科学院长春应用化学研究所 Dressing material containing medicine chitoholosida and its preparation method
CN1850291A (en) * 2006-06-09 2006-10-25 山东省医疗器械研究所 Liquid wound-dressing with functions of promoting heal of wound and bacterial-resisting
CN1944495A (en) * 2006-09-29 2007-04-11 北京大学 Water gel containing natural high molecule and its radiation preparing method
KR20120035032A (en) * 2010-10-04 2012-04-13 한국원자력연구원 Hydrogels for wound dressing comprising nano-silver particle and preparation method thereof
CN102698313A (en) * 2012-01-11 2012-10-03 北京大学 Nano-silver antibacterial hydrogel and preparation method thereof
CN103316033A (en) * 2013-07-03 2013-09-25 康晓飞 Gel and use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李进进 等: ""医用水凝胶的研究进展"", 《海外丝绸》, vol. 24, no. 3, 30 June 2009 (2009-06-30), pages 26 - 28 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103769062A (en) * 2014-02-27 2014-05-07 江南大学 Functionalized polyvinyl alcohol hydrogel and preparation method thereof
CN103769062B (en) * 2014-02-27 2017-02-01 江南大学 A functionalized polyvinyl alcohol hydrogel and preparation method
CN103893157A (en) * 2014-04-09 2014-07-02 山东赛克赛斯药业科技有限公司 Hydrogel eye protective pad dressing and preparation method thereof
CN103893157B (en) * 2014-04-09 2017-05-10 山东赛克赛斯生物科技有限公司 Eye hydrogel dressing paste and preparation method
CN105088758A (en) * 2014-05-05 2015-11-25 江苏聚威新材料有限公司 Finishing method for interweaved fabric
CN104027837A (en) * 2014-06-20 2014-09-10 东华大学 Preparation method of sodium alginate-calcium carbonate hybrid particle modified functional cotton fabric
CN105327385A (en) * 2014-08-06 2016-02-17 上海威尔医疗保健厂 Preparation method of functional hydrogel medical dressing
CN105327386A (en) * 2014-08-06 2016-02-17 上海威尔医疗保健厂 Functional hydrogel medical dressing
CN104307031A (en) * 2014-11-10 2015-01-28 刘维峰 Preparation method and usage of external use skin repair material
CN104307031B (en) * 2014-11-10 2015-12-30 刘维峰 A method of making and use of the external preparation for skin repair material
CN104474583A (en) * 2014-11-18 2015-04-01 长春吉原生物科技有限公司 Flake hydrogel material and preparation method thereof
CN105440297A (en) * 2015-04-21 2016-03-30 湖南工业大学 High-impact chitin composite gel preparation method
CN105440297B (en) * 2015-04-21 2018-01-23 湖南工业大学 A method for preparing a high impact chitin GELS
CN105239251A (en) * 2015-10-07 2016-01-13 俞松炜 Antisepsis fabric
CN106117569A (en) * 2016-06-24 2016-11-16 青岛中腾生物技术有限公司 Marine furcellaran aquagel and preparation method therefor
CN106346018A (en) * 2016-09-21 2017-01-25 武汉工程大学 Preparation method and application of agarose/nano-silver composite gel

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