CN110655292A - Compound microorganism formula - Google Patents
Compound microorganism formula Download PDFInfo
- Publication number
- CN110655292A CN110655292A CN201810705132.2A CN201810705132A CN110655292A CN 110655292 A CN110655292 A CN 110655292A CN 201810705132 A CN201810705132 A CN 201810705132A CN 110655292 A CN110655292 A CN 110655292A
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- CN
- China
- Prior art keywords
- fermentation
- compound microorganism
- protease
- polymyxa
- nocardia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
Abstract
The invention relates to a compound microorganism formula. The compound microorganism formula is prepared from the following raw materials in percentage by weight: 36-44% of bacillus subtilis, 8-12% of bryophyte, 9-11% of polymyxa, 7-9% of aspergillus, 3-6% of nocardia, 1-5% of protease, 3-5% of digestive enzyme, 3-7% of yeast, 4-7% of cellulase, 6-8% of actinomycetes and 3-5% of amylase. The compound microorganism formula provided by the invention contains various aerobic and anaerobic microorganisms, the microorganisms generate various enzymes, the unpleasant odor is eliminated, harmful microorganisms are inhibited, the BOD and COD content of leachate in excrement is reduced, the subsequent treatment load is reduced, and the compound microorganism formula is suitable for wide application range of sewage treatment in industry, agriculture, life and the like.
Description
Technical Field
The invention relates to the technical field of microbial enzymes, and particularly relates to a compound microbial formula.
Background
At present, flocculating agents are mostly used for industrial, agricultural and domestic sewage treatment, the sewage treatment is divided into three stages, the primary treatment mainly removes suspended solid pollutants in the sewage, and a grid method or a coagulating sedimentation method is generally adopted; the secondary treatment mainly removes colloidal and dissolved organic pollutants (BOD and COD substances) in the sewage; and (3) three-stage treatment, namely treating the sewage by an activated carbon adsorption method, a sand filtration method and the like. Use the flocculating agent after filtration, the pollutant after will filtering is through compression or centrifugal machine dewatering, deposit the pollutant after compression or centrifugation (national legal provisions do not allow to bury), and occupation space is great, and most water works adopt PAC as the flocculating agent, and the play water aluminium content after handling exceeds standard seriously, how can decompose the material in the sewage as far as through the microorganism, and the space that reduces to occupy is the problem that needs to solve urgently.
Due to the characteristics of the sludge, the molecular structure of the sludge is compact, the porosity is low, common beneficial zymophyte is difficult to penetrate through the sludge molecular groups, and the biological fermentation of the sludge is difficult.
Disclosure of Invention
Aiming at the defects in the prior art, the compound microorganism formula provided by the invention combines information microbiology, information life science and molecular biology, namely simply performs compound fermentation on the compound microorganism under the action of a catalyst, activates beneficial microorganisms in the environment, inhibits harmful microorganisms, performs synergistic action to realize coexistence, symbiosis and consonance, and achieves the aims of purifying and improving the environment and the like.
In order to achieve the purpose, the invention provides the following technical scheme:
the compound microorganism formula is prepared from the following raw materials in percentage by weight: 36-44% of bacillus subtilis, 8-12% of bryophyte, 9-11% of polymyxa, 7-9% of aspergillus, 3-6% of nocardia, 1-5% of protease, 3-5% of digestive enzyme, 3-7% of yeast, 4-7% of cellulase, 6-8% of actinomycetes and 3-5% of amylase.
The material is prepared from the following raw materials in parts by weight: 40% of bacillus subtilis, 10% of bryozoatum, 10% of polymyxa, 8% of aspergillus, 5% of nocardia, 3% of protease, 4% of digestive enzyme, 5% of yeast, 5% of cellulase, 7% of actinomycetes and 3% of amylase.
The material is prepared from the following raw materials in parts by weight: 44% of bacillus subtilis, 9% of bryozoatum, 9% of polymyxa, 6% of aspergillus, 4% of nocardia, 4% of protease, 3% of digestive enzyme, 7% of yeast, 6% of cellulase, 6% of actinomycetes and 2% of amylase.
The preparation method comprises the following steps:
step one, putting bacillus subtilis, bryophyte, polymyxa, nocardia, protease and microzyme into a fermentation tank for primary fermentation, adding 60-65% of water, and keeping the fermentation temperature at 25-28 ℃;
step two, the fermentation period is 30-35 days, and the first fermentation is finished;
and step three, adding aspergillus, cellulase, actinomycetes, protease and digestive enzyme into the secondary fermentation, wherein the temperature of the secondary fermentation is 45-47 ℃, the fermentation time is 7-10 days, and the preparation is finished.
The invention has the beneficial effects that: the compound microorganism formula provided by the invention combines information microbiology, information life science and molecular biology, namely simply performs compound fermentation on compound microorganisms under the action of a catalyst, activates beneficial microorganisms in the environment, inhibits harmful microorganisms, realizes coexistence, symbiosis and consortium under the synergistic effect, obtains the aims of purifying and improving the environment and the like, is diluted by water in a certain proportion after being treated by the compound microorganisms, is directly sprayed, irrigated, drunk by livestock and poultry, deodorized and environment-improved soil, prevents soil floor blocks and the like, has a sludge biological wall breaking function, can rapidly perform biological wall breaking on sludge in the sludge fermentation process, rapidly heats and evaporates water in cell membranes of the sludge under the action of the biological fermentation process, can not briefly expand gaps in sludge molecular groups, generates an aerobic fermentation friendly environment, and can sequester salt in the sludge, thereby greatly accelerating the fermentation and the propagation of the sludge.
For a better understanding of the features and technical content of the present invention, refer to the following detailed description of the invention.
Detailed Description
To further illustrate the technical means adopted by the present invention and the effects thereof, the following detailed description is given with reference to the preferred embodiments of the present invention.
The compound microorganism formula is prepared from the following raw materials in percentage by weight: 36-44% of bacillus subtilis, 8-12% of bryophyte, 9-11% of polymyxa, 7-9% of aspergillus, 3-6% of nocardia, 1-5% of protease, 3-5% of digestive enzyme, 3-7% of yeast, 4-7% of cellulase, 6-8% of actinomycetes and 3-5% of amylase.
Further, the feed additive is prepared from the following raw materials in percentage by weight: 40% of bacillus subtilis, 10% of bryozoatum, 10% of polymyxa, 8% of aspergillus, 5% of nocardia, 3% of protease, 4% of digestive enzyme, 5% of yeast, 5% of cellulase, 7% of actinomycetes and 3% of amylase.
Further, the feed additive is prepared from the following raw materials in percentage by weight: 44% of bacillus subtilis, 9% of bryozoatum, 9% of polymyxa, 6% of aspergillus, 4% of nocardia, 4% of protease, 3% of digestive enzyme, 7% of yeast, 6% of cellulase, 6% of actinomycetes and 2% of amylase.
The preparation method comprises the following steps:
step one, putting bacillus subtilis, bryophyte, polymyxa, nocardia, protease and microzyme into a fermentation tank for primary fermentation, adding 60-65% of water, and keeping the fermentation temperature at 25-28 ℃;
step two, the fermentation period is 30-35 days, and the first fermentation is finished;
and step three, adding aspergillus, cellulase, actinomycetes, protease and digestive enzyme into the secondary fermentation, wherein the temperature of the secondary fermentation is 45-47 ℃, the fermentation time is 7-10 days, and the preparation is finished.
Examples of the invention
1. The culture sewage contains a large amount of organic substances, lipids, fibers and inorganic salt components which are all substances required by the growth of microorganisms, the composite microorganisms and the enzymes produced by the microorganisms can decompose the substances into micromolecular substances, carbon dioxide and water, and the micromolecular substances are further decomposed by a large amount of microorganisms. Improving the odor treatment efficiency and reducing COD, BOD and TSS.
2. The composite microorganism is used for treating sewage discharged by a paper mill, and the data before and after treatment are as follows:
water sample | CODcr(mg/L) | NH3-N(mg/L) | Chroma (multiple) | Turbidity (NTU) | PH |
Before treatment | 15000 | 300 | 8000 | 3600 | 8.0 |
After treatment | 305.6 | 56 | 100 | 102 | 7.0 |
The composite microorganism has obvious effect of treating harmful substances in water and good effects of deodorizing, removing phosphorus and reducing NH 3-N.
3. Composite microorganism for sewage treatment of sewage plant
The sludge after the biochemical treatment of the municipal domestic sewage is black, has high water content of over 99 percent and has slight odor. By adding a small amount of compound microorganisms into the sludge, stirring at a low speed, coagulating and settling, and comparing with PAM (equal dosage), the sludge settling ratio (SV 30) of two groups of sludge samples is detected, and the test data is as follows:
water sample | SV30(%) |
Natural sedimentation | 86.92 |
Concentration of bioflocculants | 52.94 |
PAM concentration | 65.39 |
The data show that under the same dosage, the compression sedimentation ratio of the composite microorganism to the sludge can reach 52 percent, and the PAM is only 65 percent, so that the concentration performance of the composite microorganism to the sludge is stronger than that of the PAM, and in addition, after cation concentration, the sludge supernatant is clear and has no impurities, thereby being beneficial to the reflux of the supernatant.
4. Treatment of polluted water source in acid coal yard
In the coal mining process, a large amount of metals such as iron and manganese enter underground water or flow into drinking water of residents along with spring, so that the metal ions of the drinking water exceed the standard. Collecting the severely polluted water sample, wherein the appearance is light yellow,、Fe2+and Mn2+The content is seriously out of limits. The treatment scheme of aeration oxidation and (PAC + PAM) flocculation is adopted by customers, and the effect is poor. Aiming at the characteristics of the water sample, the original process is not changed, only the flocculating agent (PAC + PAM) is changed into a biological flocculating agent, and after the flocculating agent is treated, the water sample is treated、F e2+And Mn2+The content is obviously reduced, and the requirement of the metal ion content of the drinking water source specified by the state is met.
Data of the supernatant after treatment:
water sample | Iron content (mg/L) | Manganese content (mg/L) | Settling time (min) |
Raw water | 1113.5 | 8.3 | -- |
After treatment | 15 | 0.15 | 2 |
Removal Rate (%) | >98.6 | >98.1 | -- |
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (4)
1. The compound microorganism formula is characterized by being prepared from the following raw materials in parts by weight: 36-44% of bacillus subtilis, 8-12% of bryophyte, 9-11% of polymyxa, 7-9% of aspergillus, 3-6% of nocardia, 1-5% of protease, 3-5% of digestive enzyme, 3-7% of yeast, 4-7% of cellulase, 6-8% of actinomycetes and 3-5% of amylase.
2. The compound microorganism formula according to claim 1, which is prepared from the following raw materials in parts by weight: 40% of bacillus subtilis, 10% of bryozoatum, 10% of polymyxa, 8% of aspergillus, 5% of nocardia, 3% of protease, 4% of digestive enzyme, 5% of yeast, 5% of cellulase, 7% of actinomycetes and 3% of amylase.
3. The compound microorganism formula according to claim 1, which is prepared from the following raw materials in parts by weight: 44% of bacillus subtilis, 9% of bryozoatum, 9% of polymyxa, 6% of aspergillus, 4% of nocardia, 4% of protease, 3% of digestive enzyme, 7% of yeast, 6% of cellulase, 6% of actinomycetes and 2% of amylase.
4. The composite microbial formulation of claim 1, prepared by a method comprising:
step one, putting bacillus subtilis, bryophyte, polymyxa, nocardia, protease and microzyme into a fermentation tank for primary fermentation, adding 60-65% of water, and keeping the fermentation temperature at 25-28 ℃;
step two, the fermentation period is 30-35 days, and the first fermentation is finished;
and step three, adding aspergillus, cellulase, actinomycetes, protease and digestive enzyme into the secondary fermentation, wherein the temperature of the secondary fermentation is 45-47 ℃, the fermentation time is 7-10 days, and the preparation is finished.
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CN201810705132.2A CN110655292A (en) | 2018-07-01 | 2018-07-01 | Compound microorganism formula |
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CN201810705132.2A CN110655292A (en) | 2018-07-01 | 2018-07-01 | Compound microorganism formula |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111847667A (en) * | 2020-08-04 | 2020-10-30 | 武汉世纪瑞科环保科技有限公司 | Microbial deodorant and preparation method thereof |
Citations (4)
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CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
CN103613204A (en) * | 2013-11-22 | 2014-03-05 | 青岛大学 | Dirt decomposition deodorant for portable toilet |
CN106587366A (en) * | 2015-10-15 | 2017-04-26 | 百米马(天津)有限公司 | Special bacteria for leachate deodorization of garbage treatment plant and using method thereof |
WO2017146009A1 (en) * | 2016-02-24 | 2017-08-31 | 天野エンザイム株式会社 | Method for controlling enzyme productivity of microorganisms |
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- 2018-07-01 CN CN201810705132.2A patent/CN110655292A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
CN103613204A (en) * | 2013-11-22 | 2014-03-05 | 青岛大学 | Dirt decomposition deodorant for portable toilet |
CN106587366A (en) * | 2015-10-15 | 2017-04-26 | 百米马(天津)有限公司 | Special bacteria for leachate deodorization of garbage treatment plant and using method thereof |
WO2017146009A1 (en) * | 2016-02-24 | 2017-08-31 | 天野エンザイム株式会社 | Method for controlling enzyme productivity of microorganisms |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111847667A (en) * | 2020-08-04 | 2020-10-30 | 武汉世纪瑞科环保科技有限公司 | Microbial deodorant and preparation method thereof |
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