CN111847667A - Microbial deodorant and preparation method thereof - Google Patents
Microbial deodorant and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of microbial deodorization, and discloses a microbial deodorant and a preparation method thereof, wherein the microbial deodorant comprises the following raw materials in parts by weight: 20-41 parts of bacillus subtilis, 8-12 parts of bacillus, 9-11 parts of coccus, 7-9 parts of aspergillus, 3-6 parts of nocardia, 3-8 parts of protease, 3-5 parts of nitrifying enzyme, 3-7 parts of yeast, 8-12 parts of lactic acid bacteria, 4-7 parts of cellulase, 6-8 parts of actinomycetes and 3-5 parts of amylase. The microbial deodorant and the enzyme produced by the microbes can decompose organic substances, lipids, fibers, a large amount of inorganic salt components and the like into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by the large amount of microbes, so that the odor treatment effect can be improved, and the COD (chemical oxygen demand), BOD (biochemical oxygen demand) and TSS (total suspended solids) content of the aquaculture sewage can be reduced.
Description
Technical Field
The invention belongs to the technical field of microbial deodorization, and particularly relates to a microbial deodorant and a preparation method thereof.
Background
At present, a lot of products are available on the market for deodorization by using microorganisms, but the products are difficult to have a good deodorization effect in response to different complicated and changeable odor environments, and the phenomenon that the microbial deodorant cannot be used effectively often occurs.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a microbial deodorant and a preparation method thereof.
The technical scheme adopted by the invention is as follows:
a microbial deodorant comprises the following raw materials in parts by weight: 20-41 parts of bacillus subtilis, 8-12 parts of bacillus, 9-11 parts of coccus, 7-9 parts of aspergillus, 3-6 parts of nocardia, 3-8 parts of protease, 3-5 parts of nitrifying enzyme, 3-7 parts of yeast, 8-12 parts of lactic acid bacteria, 4-7 parts of cellulase, 6-8 parts of actinomycetes and 3-5 parts of amylase.
Further, the feed comprises the following raw materials in parts by weight: 20-30 parts of bacillus subtilis, 8-10 parts of bacillus, 9-10 parts of coccus, 7-8 parts of aspergillus, 3-5 parts of nocardia, 3-5 parts of protease, 3-4 parts of nitrifying enzyme, 3-5 parts of yeast, 8-10 parts of lactic acid bacteria, 4-6 parts of cellulase, 6-7 parts of actinomycetes and 3-4 parts of amylase.
Further, the feed comprises the following raw materials in parts by weight: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
Further, the feed comprises the following raw materials in parts by weight: 20 parts of bacillus subtilis, 8 parts of bacillus, 9 parts of coccus, 7 parts of aspergillus, 3 parts of nocardia, 3 parts of protease, 3 parts of nitrase, 3 parts of saccharomycete, 8 parts of lactic acid bacteria, 4 parts of cellulase, 6 parts of actinomycetes and 3 parts of amylase.
Further, the feed comprises the following raw materials in parts by weight: 30 parts of bacillus subtilis, 10 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 4 parts of nocardia, 4 parts of protease, 4 parts of nitrase, 4 parts of saccharomycete, 9 parts of lactic acid bacteria, 5 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
Further, 0.5-2 parts of monopotassium phosphate is also included.
Further, 0.5-1 part of monopotassium phosphate is also included.
The invention also discloses a preparation method of the microbial deodorant, which comprises the following steps:
taking the bacillus subtilis, bacillus, coccus, nocardia, saccharomycetes, lactobacillus and amylase in parts by mass, performing primary fermentation, and then adding 60-65 parts by mass of sterile water and 5-8 parts by mass of peptone;
fermenting in a closed manner, and opening the tank every 7 days for one minute;
after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation.
Further, the temperature of the primary fermentation is 28-35 ℃, and the time of the primary fermentation is 30-35 days.
Further, the temperature of the secondary fermentation is 32-38 ℃, and the time of the secondary fermentation is 7-10 days.
The invention has the beneficial effects that:
when the microbial deodorant is applied to deodorization treatment of aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Detailed Description
The present invention is further illustrated below with reference to specific examples.
Example 1:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 41 parts of bacillus subtilis, 12 parts of bacillus, 11 parts of coccus, 9 parts of aspergillus, 6 parts of nocardia, 8 parts of protease, 5 parts of nitrifying enzyme, 7 parts of saccharomycete, 12 parts of lactic acid bacteria, 7 parts of cellulase, 8 parts of actinomycetes and 5 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water and 6 parts by mass of peptone; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 2:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 41 parts of bacillus subtilis, 12 parts of bacillus, 11 parts of coccus, 9 parts of aspergillus, 6 parts of nocardia, 8 parts of protease, 5 parts of nitrifying enzyme, 7 parts of saccharomycete, 12 parts of lactic acid bacteria, 7 parts of cellulase, 8 parts of actinomycetes and 5 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 3:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 35 parts of bacillus subtilis, 11 parts of bacillus, 11 parts of coccus, 9 parts of aspergillus, 6 parts of nocardia, 7 parts of protease, 5 parts of nitrifying enzyme, 6 parts of saccharomycete, 11 parts of lactic acid bacteria, 7 parts of cellulase, 8 parts of actinomycetes and 5 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 4:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 35 parts of bacillus subtilis, 11 parts of bacillus, 11 parts of coccus, 9 parts of aspergillus, 6 parts of nocardia, 6 parts of protease, 5 parts of nitrifying enzyme, 6 parts of saccharomycete, 11 parts of lactic acid bacteria, 7 parts of cellulase, 8 parts of actinomycetes and 5 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 5:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 6:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 20 parts of bacillus subtilis, 8 parts of bacillus, 9 parts of coccus, 7 parts of aspergillus, 3 parts of nocardia, 3 parts of protease, 3 parts of nitrase, 3 parts of saccharomycete, 8 parts of lactic acid bacteria, 4 parts of cellulase, 6 parts of actinomycetes and 3 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 7:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 30 parts of bacillus subtilis, 10 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 4 parts of nocardia, 4 parts of protease, 4 parts of nitrase, 4 parts of saccharomycete, 9 parts of lactic acid bacteria, 5 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 30 ℃, and the time of the primary fermentation is 35 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 35 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 8:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 60 parts by mass of sterile water, 5 parts by mass of peptone and 0.5 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 28 ℃, and the time of the primary fermentation is 30 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 32 ℃, and the time of the secondary fermentation is 10 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 9:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, bacillus, coccus, nocardia, saccharomycetes, lactobacillus and amylase in parts by mass, performing primary fermentation, and then adding 65 parts by mass of sterile water, 8 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 35 ℃, and the time of the primary fermentation is 32 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 38 ℃, and the time of the secondary fermentation is 7 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
Example 10:
the embodiment provides a microbial deodorant, which comprises the following raw materials in parts by mass: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
The preparation method of the microbial deodorant comprises the following steps:
(1) taking the bacillus subtilis, the bacillus, the coccus, the nocardia, the microzyme, the lactic acid bacteria and the amylase in parts by mass, performing primary fermentation, and then adding 62.5 parts by mass of sterile water, 6 parts by mass of peptone and 1 part by mass of potassium dihydrogen phosphate; wherein the temperature of the primary fermentation is 28 ℃, and the time of the primary fermentation is 30 days.
(2) Performing closed fermentation in the tank body in the primary fermentation process, and opening the tank for one minute every 7 days;
(3) after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation. The temperature of the secondary fermentation is 32 ℃, and the time of the secondary fermentation is 10 days. And (5) obtaining the microbial deodorant after the secondary fermentation is completed.
The microbial deodorant prepared by the invention combines information microbiology, information life science and molecular biology, namely simply performs composite fermentation on a plurality of selected microbes under the action of a catalyst, activates beneficial microbes in the environment, inhibits harmful microbes (added biological enzyme in the microbial deodorant can promote quick breeding of the microbes, and mass multiplication of the microbes can extrude living space of the harmful microbes on the surface of a foul source environment to play a role in inhibiting the harmful microbes), and realizes coexistence, symbiosis and consortium under the synergistic effect, so that the aims of purifying and improving the environment are achieved.
When the microbial deodorant is applied to deodorization treatment of the aquaculture sewage, the aquaculture sewage contains a large amount of organic substances, lipids, fibers and a large amount of inorganic salt components which are all substances required by microbial growth, the microbial deodorant and enzymes generated by microbes can decompose the organic substances, the lipids, the fibers and the large amount of inorganic salt components into small molecular substances, carbon dioxide and water, and the small molecular substances are further decomposed by a large amount of microbes, so that the odor treatment effect can be improved, and the COD, BOD and TSS contents of the aquaculture sewage can be reduced.
The application method of the microbial deodorant of the invention is as follows: the microbial deodorant prepared in any one of the embodiments 1-10 is selected, the mass ratio of the microbial deodorant to water is 1: 500-: the garbage pile, a sewer, a septic tank, a farm, an odorous ditch or a black and odorous river and the like are sprayed once every 24 hours, and after the garbage pile, the septic tank, the farm, the odorous ditch or the black and odorous river are continuously used for 1 week, the odor emitted by an area to be deodorized can be basically removed.
When the microbial deodorant acts on an area to be deodorized, microorganisms in the microbial deodorant take organic matters such as protein, starch, grease and the like in an odor source as breeding food, and begin to breed in large quantities, so that stubborn organic matters can be degraded and peculiar smell can be removed; meanwhile, the microorganisms can implant themselves into the organic matter deposition (the microorganisms are sprayed on the surface of the organic matter and quickly start to multiply on the surface of the organic matter, and the microorganism population can multiply to the periphery of the organic matter and the interior with higher organic matter content, namely, the microorganism population is implanted into the organic matter deposition), so that the substances generating peculiar smell can be degraded and reduced for a long time.
TABLE 1 data statistics table for treating hydrogen sulfide and ammonia etc. in garbage transfer station by the microbial deodorant of the present invention
As can be seen from Table 1, the microbial deodorant of the invention has an obvious deodorizing effect on harmful substances in a garbage transfer station, and has a removal rate of hydrogen sulfide, ammonia, methyl mercaptan and odor of more than 99%.
TABLE 2 microbial deodorant of the invention vs. CODcr and NH in a refuse transfer station3-N, etc. of the data statistics
| Water sample | CODcr(mg/L) | NH3-N(mg/L) | Chroma (multiple) | Turbidity (NTU) |
| Before treatment | 10000 | 1500 | 7000 | 3000 |
| After treatment | 284.1 | 44 | 100 | 85 |
As can be seen from Table 2, the microbial deodorant of the present invention has a remarkable deodorizing effect on the harmful substances in water, and can deodorize, remove CODcr and reduce NH3The effect of-N is good.
The present invention is not limited to the above-described alternative embodiments, and various other forms of products can be obtained by anyone in light of the present invention. The above detailed description should not be taken as limiting the scope of the invention, which is defined in the claims, and which the description is intended to be interpreted accordingly.
Claims (10)
1. The microbial deodorant is characterized by comprising the following raw materials in parts by mass: 20-41 parts of bacillus subtilis, 8-12 parts of bacillus, 9-11 parts of coccus, 7-9 parts of aspergillus, 3-6 parts of nocardia, 3-8 parts of protease, 3-5 parts of nitrifying enzyme, 3-7 parts of yeast, 8-12 parts of lactic acid bacteria, 4-7 parts of cellulase, 6-8 parts of actinomycetes and 3-5 parts of amylase.
2. The microbial deodorant according to claim 1, which comprises the following raw materials in parts by mass: 20-30 parts of bacillus subtilis, 8-10 parts of bacillus, 9-10 parts of coccus, 7-8 parts of aspergillus, 3-5 parts of nocardia, 3-5 parts of protease, 3-4 parts of nitrifying enzyme, 3-5 parts of yeast, 8-10 parts of lactic acid bacteria, 4-6 parts of cellulase, 6-7 parts of actinomycetes and 3-4 parts of amylase.
3. The microbial deodorant according to claim 2, which comprises the following raw materials in parts by mass: 25 parts of bacillus subtilis, 9 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 5 parts of nocardia, 5 parts of protease, 4 parts of nitrifying enzyme, 5 parts of saccharomycete, 10 parts of lactic acid bacteria, 6 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
4. The microbial deodorant according to claim 2, which comprises the following raw materials in parts by mass: 20 parts of bacillus subtilis, 8 parts of bacillus, 9 parts of coccus, 7 parts of aspergillus, 3 parts of nocardia, 3 parts of protease, 3 parts of nitrase, 3 parts of saccharomycete, 8 parts of lactic acid bacteria, 4 parts of cellulase, 6 parts of actinomycetes and 3 parts of amylase.
5. The microbial deodorant according to claim 2, which comprises the following raw materials in parts by mass: 30 parts of bacillus subtilis, 10 parts of bacillus, 10 parts of coccus, 8 parts of aspergillus, 4 parts of nocardia, 4 parts of protease, 4 parts of nitrase, 4 parts of saccharomycete, 9 parts of lactic acid bacteria, 5 parts of cellulase, 7 parts of actinomycetes and 4 parts of amylase.
6. The microbial deodorant according to claim 1, wherein: also comprises 0.5 to 2 portions of monopotassium phosphate.
7. The microbial deodorant according to claim 6, wherein: also comprises 0.5 to 1 portion of monopotassium phosphate.
8. The method for producing a microbial deodorant according to any one of claims 1 to 7, characterized by comprising the steps of:
taking the bacillus subtilis, bacillus, coccus, nocardia, saccharomycetes, lactobacillus and amylase in parts by mass, performing primary fermentation, and then adding 60-65 parts by mass of sterile water and 5-8 parts by mass of peptone;
fermenting in a closed manner, and opening the tank every 7 days for one minute;
after the primary fermentation is finished, adding the aspergillus, cellulase, actinomycetes, protease and nitrase in parts by weight for secondary fermentation.
9. The method for producing a microbial deodorant according to claim 8, characterized in that: the temperature of the primary fermentation is 28-35 ℃, and the time of the primary fermentation is 30-35 days.
10. The method for producing a microbial deodorant according to claim 9, characterized in that: the temperature of the secondary fermentation is 32-38 ℃, and the time of the secondary fermentation is 7-10 days.
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| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN110052159A (en) * | 2019-05-21 | 2019-07-26 | 广西彼得大地生物科技有限公司 | A kind of new type of safe high-performance bio deodorant and preparation method thereof |
| CN110655292A (en) * | 2018-07-01 | 2020-01-07 | 才莉 | Compound microorganism formula |
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| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN110655292A (en) * | 2018-07-01 | 2020-01-07 | 才莉 | Compound microorganism formula |
| CN110052159A (en) * | 2019-05-21 | 2019-07-26 | 广西彼得大地生物科技有限公司 | A kind of new type of safe high-performance bio deodorant and preparation method thereof |
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