Liquid separation device and method thereof
Technical Field
The invention relates to a liquid separation device and a method thereof.
Background
Because the number of cells can be increased continuously in the culture process, the culture container needs to be replaced or the liquid separation treatment needs to be carried out in the middle and later stages of the culture, and the existing liquid separation treatment is basically carried out manually, so that the labor intensity is high, and the controllability is low.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a liquid separation device and a method thereof.
The technical scheme of the invention is as follows:
a liquid separation device, comprising: the liquid storage device comprises a base, a lower bearing jig for bearing a liquid taking container, an upper bearing jig for bearing a liquid storage container, a shunt, a middle bearing jig for mounting the shunt, and a driving assembly fixed on the base; the upper end of the flow divider is provided with a liquid inlet end for collecting liquid discharged by the liquid storage container, and the lower end of the flow divider is provided with a liquid outlet end for communicating the liquid taking container; the middle bearing jig is fixedly connected with the driving end of the driving assembly; the driving component drives the bearing jig to move upwards or downwards.
The further technical scheme is as follows: the driving assembly drives the bearing jig to move downwards so that the liquid outlet end of the flow divider is communicated with the liquid collecting port formed in the liquid taking container.
The further technical scheme is as follows: the liquid storage container is provided with a liquid discharge pipe; the liquid discharge pipe is provided with a liquid discharge end; the driving assembly drives the bearing jig to move upwards so that the liquid discharge end of the liquid discharge pipe is communicated with the liquid inlet end of the flow divider.
The further technical scheme is as follows: the liquid storage container comprises a shell, a culture bag and a pipeline plugging component which is connected to the shell in a sliding mode; the culture bag is provided with the liquid discharge pipe; the shell is provided with a containing space for containing the culture bag and a pipeline groove for passing through the liquid discharge pipe; the pipeline plugging component is arranged on one side of the pipeline groove and elastically presses the liquid discharge pipe on the shell; when the pipeline plugging component elastically compresses the liquid discharge pipe on the shell, the liquid discharge pipe is closed.
The further technical scheme is as follows: the liquid separating device also comprises an installation part for fixing the upper bearing jig and an upper liquid control assembly; the upper liquid control assembly comprises a linear driving unit fixed on the mounting piece and a control end; the linear driving unit drives the control end to be close to or far away from the liquid storage container; the control end presses the pipeline plugging component to move so as to enable the liquid discharge pipe to be conducted.
The further technical scheme is as follows: the liquid taking container and the liquid storage container have the same structure; the liquid separating device is also provided with a lower liquid control assembly positioned on one side of the liquid taking container; the lower liquid control assembly and the upper liquid control assembly are identical in structure.
The further technical scheme is as follows: the flow divider is a funnel.
The further technical scheme is as follows: the driving assembly is an electric screw rod or a linear cylinder and a linear motor.
The further technical scheme is as follows: the liquid taking container is a tubular container.
The further technical scheme is as follows: the liquid distribution device further comprises a weighing sensor arranged at the bottom of the lower bearing jig.
A liquid separation method adopts the liquid separation device, and comprises the following steps: mounting the liquid taking container to the lower bearing jig; installing a liquid storage container to the upper bearing jig; the driving assembly drives the bearing jig to move upwards so that the liquid discharge end of the liquid discharge pipe is communicated with the liquid inlet end of the flow divider; the driving component drives the bearing jig to move downwards so as to enable the liquid outlet end of the flow divider to be communicated with the liquid taking container; and controlling the liquid stored in the liquid storage container discharger to be transferred to the liquid taking container through the flow divider.
Compared with the prior art, the invention has the technical effects that: the utility model provides a liquid distribution device, the stock solution container that needs divide liquid sets up on last bearing jig, gets the liquid container and then sets up under and bear on the jig, because the container that divides liquid probably is the culture bag during cell culture, also can be the test tube, consequently, the shunt adopts the drive assembly drive to remove to make the shunt can shift the liquid that the stock solution container discharged to get the liquid container. The liquid separating device provided by the invention replaces manual liquid separation, reduces the labor intensity, and has strong controllability and adaptability.
The liquid separation method is high in automation degree and strong in controllability by adopting the liquid separation device.
The invention is further described below with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a perspective view of a dispensing device of the present invention;
FIG. 2 is an exploded view of the upper fluid control assembly and fluid reservoir of the embodiment of FIG. 1;
FIG. 3 is a flow chart of a method of dispensing.
Reference numerals
10 divide liquid device 1 base
2 get liquid container 3 and bear tool down
4 liquid storage container 41 shell
411 pipeline trough 42 culture bag
421 liquid discharge pipe 43 pipeline plugging assembly
5 go up and bear 6 shunts of tool
61 liquid inlet end and 62 liquid outlet end
7-medium bearing jig 8 driving assembly
91 installation part 92 upper liquid control assembly
921 control end of linear driving unit 922
93 lower liquid control assembly
Detailed Description
In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further described and illustrated below with reference to the schematic drawings, but not limited thereto.
A liquid separation device, comprising: the liquid storage device comprises a base, a lower bearing jig for bearing a liquid taking container, an upper bearing jig for bearing a liquid storage container, a shunt, a middle bearing jig for mounting the shunt, and a driving assembly fixed on the base. The upper end of the flow divider is provided with a liquid inlet end for collecting liquid discharged by the liquid storage container, and the lower end of the flow divider is provided with a liquid outlet end for communicating the liquid taking container. The middle bearing jig is fixedly connected with the driving end of the driving component, and the bearing jig moves upwards or downwards in the driving of the driving component so that the shunt is communicated with the liquid storage container or the liquid taking container.
As shown in fig. 1, a liquid dispensing device 10 includes: the liquid storage device comprises a base 1, a lower bearing jig 3 used for bearing a liquid taking container 2, an upper bearing jig 5 used for bearing a liquid storage container 4, a flow divider 6, a middle bearing jig 7 used for installing the flow divider 6 and a driving assembly 8 fixed on the base 1. The upper end of the flow divider 6 is provided with a liquid inlet end 61 for collecting liquid discharged by the liquid storage container 4, and the lower end of the flow divider 6 is provided with a liquid outlet end 62 for communicating the liquid taking container 2. The middle bearing jig 7 is fixedly connected with the driving end of the driving component 8, and the driving component 8 drives the middle bearing jig 7 to move upwards or downwards so that the shunt 6 is communicated with the liquid storage container 4 or the liquid taking container 2.
Preferably, the liquid storage container 4 is provided with a liquid discharge pipe 421, and the liquid discharge pipe 421 is provided with a liquid discharge end. The driving assembly 8 drives the middle bearing jig 7 to move upwards, so that the liquid discharge end of the liquid discharge pipe 421 is communicated with the liquid inlet end 61 of the flow divider 6, and the middle bearing jig 7 moves upwards to prepare for action, so that liquid discharged by the liquid discharge pipe 421 can flow into the flow divider 6. In specific implementation, the liquid storage container 4 is a culture bag.
Preferably, the flow divider 6 is a funnel, and the caliber of the upper end is larger than that of the lower end. Bearing jig 7 moves downwards in the drive of drive assembly 8 to make the play liquid end 62 of shunt 6 switch on the album liquid mouth that gets liquid container 2 and be equipped with, during specific implementation, get liquid container 2 and can be culture bag 42 also can be tubular container, like the test tube, in this embodiment, get liquid container and be the test tube. During liquid separation, the lower end of the funnel can be extended into the test tube. If the liquid taking container is a culture bag provided with a liquid inlet pipe, the liquid outlet end of the flow divider needs to be connected and communicated with the liquid inlet pipe of the culture bag. The lower bearing jig is divided into two types which can be used for bearing tubular containers and culture bags.
Specifically, the middle bearing jig 7 is provided with a sliding groove for mounting the shunt 6, and the shunt 6 is provided with a sliding connection part in sliding connection with the sliding groove.
Specifically, the driving assembly 8 is an electric screw rod or a linear cylinder, a linear motor, and in this embodiment, the driving assembly 8 is a linear cylinder.
Preferably, the liquid separating device 10 further comprises a mounting member 91 for fixing the upper bearing jig 5, an upper liquid control assembly 92, and a lower liquid control assembly 93 positioned on one side of the liquid taking container 2, wherein the lower liquid control assembly 93 has the same structure as the upper liquid control assembly 92.
As shown in FIG. 2, the liquid storage container 4 comprises a case 41, a culture bag 42, and a tube plugging member 43 slidably attached to the case 41, wherein the culture bag 42 is provided with a drain 421. The case 41 is provided with a receiving space for receiving the culture bag 42, and a pipe tank 411 for passing through the drain pipe 421, and the case 41 includes a front case 411 and a rear case 412. The tube plugging member 43 is disposed at one side of the tube slot 411 and elastically presses the drainage tube 421 against the housing 41, and when the tube plugging member 43 elastically presses the drainage tube 421 against the housing 41, the drainage tube 421 is closed, and at this time, the liquid in the culture bag 42 cannot flow out.
Preferably, the upper liquid control assembly 92 includes a linear driving unit 921 fixed to the mounting member 91, and a control end 922. The linear driving unit 921 drives the control end 922 to move closer to or away from the liquid storage container 4, and when the control end 922 presses the pipeline plugging component 43 to move, the pipeline plugging component 43 releases the compression on the liquid discharge pipe 421, so that the liquid discharge pipe 421 is conducted, and at this time, the liquid in the culture bag 42 can flow out.
Preferably, an elastic member is disposed between the pipe plugging member 43 and the housing 41, and when the control end 922 does not apply an external force to the pipe plugging member 43, the pipe plugging member 43 presses the drain pipe 421 against the housing 41 all the time under the action of the elastic member.
Preferably, divide liquid device 10 still including locate down the load cell who bears the weight of tool 3 bottom, load cell is connected with last accuse liquid subassembly 92 electricity, weighs the weight of reposition of redundant personnel through load cell to reach the purpose of control branch liquid measure.
During concrete implementation, the bearing jig moves upwards in the drive assembly drive, and after the flowing back end of fluid-discharge tube switched on with the feed liquor end of shunt, the bearing jig moved downwards in the drive assembly can continue the drive, and at this moment, the flowing back end of shunt and fluid-discharge tube can separate, and after pipeline shutoff subassembly relieved compressing tightly the fluid-discharge tube, the liquid that the fluid-discharge tube flowed also can flow into in the shunt of below under the condition of dead weight.
When the liquid taking container is also a culture bag, the liquid taking container is controlled to be communicated through the lower liquid control assembly.
As shown in fig. 3, a liquid separation method using the liquid separation device includes:
s1, mounting the liquid taking container to a lower bearing jig;
s2, mounting a liquid storage container to the upper bearing jig;
s3, driving the bearing jig to move upwards by the driving assembly so as to enable the liquid discharge end of the liquid discharge pipe to be communicated with the liquid inlet end of the flow divider;
s4, the driving component drives the bearing jig to move downwards so that the liquid outlet end of the flow divider is communicated with the liquid taking container;
and S5, controlling the liquid stored in the liquid storage container discharger and transferring the liquid to the liquid taking container through the flow divider.
The technical contents of the present invention are further illustrated by the examples to facilitate the understanding of the reader, but the embodiments of the present invention are not limited thereto, and any technical extension or re-creation based on the present invention is protected by the present invention, and the scope of the present invention is subject to the claims.