CN110638680A - 一种槲皮素负载香菇菌多糖复合物的制备方法及应用 - Google Patents
一种槲皮素负载香菇菌多糖复合物的制备方法及应用 Download PDFInfo
- Publication number
- CN110638680A CN110638680A CN201910782606.8A CN201910782606A CN110638680A CN 110638680 A CN110638680 A CN 110638680A CN 201910782606 A CN201910782606 A CN 201910782606A CN 110638680 A CN110638680 A CN 110638680A
- Authority
- CN
- China
- Prior art keywords
- quercetin
- loaded
- lentinan
- complex
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 title claims abstract description 95
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 title claims abstract description 47
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 title claims abstract description 47
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229960001285 quercetin Drugs 0.000 title claims abstract description 47
- 235000005875 quercetin Nutrition 0.000 title claims abstract description 47
- 240000000599 Lentinula edodes Species 0.000 title claims abstract description 21
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 19
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 150000004676 glycans Chemical class 0.000 title claims description 10
- 235000001715 Lentinula edodes Nutrition 0.000 title claims description 6
- 229920001491 Lentinan Polymers 0.000 claims abstract description 25
- 229940115286 lentinan Drugs 0.000 claims abstract description 25
- -1 polysaccharide compound Chemical class 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 206010061218 Inflammation Diseases 0.000 claims description 10
- 244000269722 Thea sinensis Species 0.000 claims description 10
- 230000004054 inflammatory process Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 8
- 235000013616 tea Nutrition 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 238000007254 oxidation reaction Methods 0.000 claims description 5
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000003647 oxidation Effects 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 3
- 230000003712 anti-aging effect Effects 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 239000000443 aerosol Substances 0.000 claims description 2
- 125000000129 anionic group Chemical group 0.000 claims description 2
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 235000009569 green tea Nutrition 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 239000007764 o/w emulsion Substances 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 238000007670 refining Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 229960004793 sucrose Drugs 0.000 claims description 2
- 239000007762 w/o emulsion Substances 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims 2
- 239000003963 antioxidant agent Substances 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 claims 1
- 235000006708 antioxidants Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002537 cosmetic Substances 0.000 abstract description 3
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 18
- 230000000694 effects Effects 0.000 description 11
- 230000006872 improvement Effects 0.000 description 10
- WTVHAMTYZJGJLJ-UHFFFAOYSA-N (+)-(4S,8R)-8-epi-beta-bisabolol Natural products CC(C)=CCCC(C)C1(O)CCC(C)=CC1 WTVHAMTYZJGJLJ-UHFFFAOYSA-N 0.000 description 9
- RGZSQWQPBWRIAQ-CABCVRRESA-N (-)-alpha-Bisabolol Chemical compound CC(C)=CCC[C@](C)(O)[C@H]1CCC(C)=CC1 RGZSQWQPBWRIAQ-CABCVRRESA-N 0.000 description 9
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 description 9
- RGZSQWQPBWRIAQ-LSDHHAIUSA-N alpha-Bisabolol Natural products CC(C)=CCC[C@@](C)(O)[C@@H]1CCC(C)=CC1 RGZSQWQPBWRIAQ-LSDHHAIUSA-N 0.000 description 9
- 229940036350 bisabolol Drugs 0.000 description 9
- HHGZABIIYIWLGA-UHFFFAOYSA-N bisabolol Natural products CC1CCC(C(C)(O)CCC=C(C)C)CC1 HHGZABIIYIWLGA-UHFFFAOYSA-N 0.000 description 9
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 description 9
- 239000002158 endotoxin Substances 0.000 description 9
- 229960000890 hydrocortisone Drugs 0.000 description 9
- 229920006008 lipopolysaccharide Polymers 0.000 description 9
- 238000001514 detection method Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000008961 swelling Effects 0.000 description 7
- 210000003371 toe Anatomy 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 102000003777 Interleukin-1 beta Human genes 0.000 description 3
- 108090000193 Interleukin-1 beta Proteins 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000002757 inflammatory effect Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 210000000707 wrist Anatomy 0.000 description 3
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 241000243251 Hydra Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 239000000679 carrageenan Substances 0.000 description 2
- 229920001525 carrageenan Polymers 0.000 description 2
- 235000010418 carrageenan Nutrition 0.000 description 2
- 229940113118 carrageenan Drugs 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 210000002683 foot Anatomy 0.000 description 2
- 239000003862 glucocorticoid Substances 0.000 description 2
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 230000036572 transepidermal water loss Effects 0.000 description 2
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 2
- PAFLSMZLRSPALU-MRVPVSSYSA-N (2R)-3-(3,4-dihydroxyphenyl)lactic acid Chemical compound OC(=O)[C@H](O)CC1=CC=C(O)C(O)=C1 PAFLSMZLRSPALU-MRVPVSSYSA-N 0.000 description 1
- GOZMBJCYMQQACI-UHFFFAOYSA-N 6,7-dimethyl-3-[[methyl-[2-[methyl-[[1-[3-(trifluoromethyl)phenyl]indol-3-yl]methyl]amino]ethyl]amino]methyl]chromen-4-one;dihydrochloride Chemical compound Cl.Cl.C=1OC2=CC(C)=C(C)C=C2C(=O)C=1CN(C)CCN(C)CC(C1=CC=CC=C11)=CN1C1=CC=CC(C(F)(F)F)=C1 GOZMBJCYMQQACI-UHFFFAOYSA-N 0.000 description 1
- 206010007191 Capillary fragility Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- PAFLSMZLRSPALU-QMMMGPOBSA-N Danshensu Natural products OC(=O)[C@@H](O)CC1=CC=C(O)C(O)=C1 PAFLSMZLRSPALU-QMMMGPOBSA-N 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102000000589 Interleukin-1 Human genes 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- PAFLSMZLRSPALU-UHFFFAOYSA-N Salvianic acid A Natural products OC(=O)C(O)CC1=CC=C(O)C(O)=C1 PAFLSMZLRSPALU-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000010936 aqueous wash Methods 0.000 description 1
- 210000000544 articulatio talocruralis Anatomy 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
本发明属于生物化学技术领域，具体涉及一种槲皮素负载香菇菌多糖复合物的制备方法及应用。槲皮素负载香菇菌多糖复合物包括按重量百分含量组分的原料：香菇菌多糖，含量80‑99％；槲皮素，含量1％‑20％。本发明的槲皮素负载香菇菌多糖复合物制备过程，可以使得槲皮素的稳定性得到很大提高，而且在化妆品中实现缓慢释放。
Description
技术领域
本发明属于生物化学技术领域，具体涉及一种槲皮素负载香菇菌多糖复合物的制备方法及应用。
背景技术
香菇菌多糖，它既是水溶性的，又是电中性的，因此更加适合于化妆品的应用，其拥有优异的抗衰老功效，能够抚平细小皱纹，改善皮肤纹理度。赋予了产品良好的透皮吸收性能，可作为活性成份的释放载体，给予皮肤如丝绸般滋润光滑的触感。
槲皮素，又称槲皮黄素,黄色针状晶体，槲皮素属黄酮类化合物，多以甙的形式存在，具有较好的祛痰、止咳作用，并有一定的平喘作用。此外还有降低血压、增强毛细血管脆性等功效。
痤疮等皮肤问题可由炎症引起，通过抑制炎症并改善皮肤状态可有效改善痤疮、皮肤衰老等问题。在护肤产品中，往往需要搭配多种来产生协同作用起到抑制炎症同时改善皮肤。
发明内容
本发明的一个目的是提供一种新型槲皮素负载香菇菌多糖复合物的制备方法
本发明的另一个目的是融合香菇菌多糖和丹参素，使二者协同作用用于护肤产品及药物上的研发及应用。
为实现上述目的，本发明采用如下技术方案，本发明槲皮素负载香菇菌多糖复合物，包括按重量百分含量组分的原料：香菇菌多糖，含量80-99％；槲皮素，含量1％-20％。
所述香菇菌多糖，又称香菇多糖，Lentinan，CAS:37339-90-5，分子量：1152.9995，灰白色粉末；所述槲皮素，又称槲皮黄素，Quercetin，CAS:117-39-5，分子量：302，黄色针状结晶。
上述槲皮素负载香菇菌多糖复合物的制备方法，所述香菇菌多糖采用生物发酵后提取方式制备，然后与槲皮素通过化学合成方式获得槲皮素负载香菇菌多糖复合物复合物，包括以下步骤：
步骤一，以新鲜绿茶茶叶为主，蔗糖、蛋白胨、磷酸氢钾等为辅，按照茶叶与水重量比为1:1的比例使茶叶和水充分混匀；在121℃高温条件下充分灭菌20-30分钟后得到茶叶培养基；将所述茶叶培养基与香菇菌丝体共培养；对香菇菌丝体的发酵产物水提醇沉分离提取，最终精制得到香菇菌多糖；
步骤二，香菇菌多糖与槲皮素以相应的比例混合在去离子水中；加入与槲皮素等当量的二环己基碳二亚胺作为缩合剂；在室温下搅拌反应24h；透析24小时，除去多余二环己基碳二亚胺以及未反应的槲皮素；冷冻干燥，最终得到相应的槲皮素负载香菇菌多糖复合物。
进一步的，所述香菇菌丝体采用的香菇菌品种不限于某单一品种的香菇菌。
所述的槲皮素负载香菇菌多糖复合物主要以水性糊剂或凝胶或液体的形式，例如水性液体的形式。
优选地，将槲皮素负载香菇菌多糖复合物配制为含水洗液、油包水或水包油乳液、油或油.醇洗液、阴离子或非离子两亲脂质的囊状分散体、含水凝胶、水.醇凝胶、醇凝胶或油.醇凝胶、固体棒或气溶胶。
本发明将槲皮素负载香菇菌多糖复合物用于抗炎、抗氧化衰老、护肤的制剂。
进一步的，所述剂型为临床上可接受的任一剂型。
进一步的，所述槲皮素负载香菇菌多糖复合物为所述抗炎、抗氧化衰老或护肤的唯一活性成分，或该复合物与其他物质一起制备所述抗炎、抗氧化衰老或护肤的制剂。
本发明与现有技术相比，本发明的槲皮素负载香菇菌多糖复合物制备过程，可以使得槲皮素的稳定性得到很大提高，而且在化妆品中实现缓慢释放。
附图说明
图1.DT004对炎症因子TNF-α和IL-1β的影响；
图2.DT004对小鼠足趾肿胀的影响。
具体实施方式
下面将结合具体的实施方案对本发明进行进一步的解释，但并不局限本发明，在本领域普通技术人员所具备的知识范围内，还可以在不脱离本发明宗旨的前提下作出各种变化。
下述实施例中，如无特殊说明，所用方法为常规方法，所用试剂都来源于市售商品。
实施例1.槲皮素负载香菇菌多糖复合物对炎症因子TNF-α和IL-1β的影响检测
检测方法：
小鼠巨噬细胞RAW264.7接种于12孔板内，37℃，5％CO2培养箱培养24h后，加入40mg/L脂多糖(LPS)作用4h诱导炎症反应，适当浓度的DT004、GK2、Drag和Hydr于LPS加入前2h加入。LPS作用4h后，收集细胞上清，按照ELISA试剂盒说明书测定TNF-α和IL-1β的含量。
结果如图1所示，DT004：槲皮素负载香菇菌多糖复合物；LPS：脂多糖；GK2：甘草酸二钾；Drag：红没药醇；Hydr：氢化可的松。(n＝3，**P<0.01)
结论：
DT004显著抑制LPS诱导引起的炎症因子TNF-α和IL-1β含量的上升，且效果显著优于阳性药甘草酸二钾和红没药醇，其抑炎效果与糖皮质激素氢化可的松效果相当。
实施例2.对小鼠足趾肿胀的影响检测
检测方法：
1％羧甲基纤维素钠配药，使成凝胶状，并在每鼠右下足趾正反两面均匀涂抹药物。半小时后，每鼠左右两足趾均皮下注射1％角叉菜胶约0.025mL，空白对照组小鼠不做处理。5h后脱颈处死，沿踝关节减下左右两足，称重，计算肿胀度及肿胀率，并做统计分析。
肿胀度＝炎症组足趾重量-空白组足趾重量
结果如图2所示。DT004：槲皮素负载香菇菌多糖复合物；LPS：脂多糖；GK2：甘草酸二钾；Drag：红没药醇；Hydr：氢化可的松。(n＝3，**P<0.01)
结论：
DT004显著抑制角叉菜胶对小鼠足趾造成的肿胀，且效果显著优于阳性药甘草酸二钾和红没药醇，其抑炎效果略优于糖皮质激素氢化可的松。
实施例3.DT004对皮肤改善情况的检测
检测方法：
招募30名志愿者(其中15男，15女)，随机分为5组(对照、DT004、GK2、Drag、Hydr)，其中每组男女各3人。每次使用时先用湿巾或水洗将右手手腕内侧清理干净，随后护肤品均匀擦拭于右手手腕内侧部位，每天早晚各一次，连续使用4周共28天。分别于第0、7、14、28天使用DermaLab仪器重点检测并监测了TEWL(经表皮水流失)、Hydration PN(水含量)、Ultrasound(超声)、Elasticity(弹性)和Skin Color(肤色)共五项指标，每次检测时志愿者右手手腕内侧湿巾擦拭干净并晾晒20分钟左右至晾干后开始检测。仪器所在房间不通风，无阳光直射，室温保持在24-25℃，湿度保持在40％。最后对得到的数据做统计分析。
结果：
表1.对照组皮肤改善情况：
表2.DT004对皮肤的改善情况(DT004：槲皮素负载香菇菌多糖复合物)：
表3.甘草酸二钾对皮肤的改善情况(GK2：甘草酸二钾)：
表4.红没药醇对皮肤的改善情况(Drag：红没药醇)：
表5.氢化可的松对皮肤的改善情况(Hydr：氢化可的松)：
结论：
DT004可有效改善皮肤的保水性能，提高皮层胶原蛋白含量，改善皮肤弹性。对保水性能的最高改善率优于红没药醇，与甘草酸二钾和氢化可的松效果相当；对胶原蛋白含量的改善优于三种阳性药；对皮肤弹性的最高改善率优于氢化可的松，与甘草酸二钾和红没药醇效果相当。与对照组比较，DT004对皮肤的改善效果优于甘草酸二钾、红没药醇和氢化可的松。
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型，本发明的范围由所附权利要求及其等同物限定。
Claims (9)
1.槲皮素负载香菇菌多糖复合物，其特征在于：包括按重量百分含量组分的原料：
香菇菌多糖，含量80-99％；
槲皮素，含量1％-20％。
2.根据权利要求1所述的槲皮素负载香菇菌多糖复合物，其特征在于：
所述香菇菌多糖，又称香菇多糖，Lentinan，CAS:37339-90-5，分子量：1152.9995，灰白色粉末；
所述槲皮素，又称槲皮黄素，Quercetin，CAS:117-39-5，分子量：302，黄色针状结晶。
3.权利要求1所述的槲皮素负载香菇菌多糖复合物的制备方法，其特征在于，所述香菇菌多糖采用生物发酵后提取方式制备，然后与槲皮素通过化学合成方式获得槲皮素负载香菇菌多糖复合物复合物，包括以下步骤：
步骤一，以新鲜绿茶茶叶为主，蔗糖、蛋白胨、磷酸氢钾等为辅，按照茶叶与水重量比为1:1的比例使茶叶和水充分混匀；在121℃高温条件下充分灭菌20-30分钟后得到茶叶培养基；将所述茶叶培养基与香菇菌丝体共培养；对香菇菌丝体的发酵产物水提醇沉分离提取，最终精制得到香菇菌多糖；
步骤二，香菇菌多糖与槲皮素以相应的比例混合在去离子水中；加入与槲皮素等当量的二环己基碳二亚胺作为缩合剂；在室温下搅拌反应24h；透析24小时，除去多余二环己基碳二亚胺以及未反应的槲皮素；冷冻干燥，最终得到相应的槲皮素负载香菇菌多糖复合物。
4.根据权利要求3所述的制备方法，其特征在于：所述香菇菌丝体采用的香菇菌品种不限于某单一品种的香菇菌。
5.根据权利要求1所述的槲皮素负载香菇菌多糖复合物，其特征在于：主要以水性糊剂或凝胶或液体的形式，优选为水性液体的形式。
6.根据权利要求5所述的槲皮素负载香菇菌多糖复合物，其特征在于：将槲皮素负载香菇菌多糖复合物配制为含水洗液、油包水或水包油乳液、油或油.醇洗液、阴离子或非离子两亲脂质的囊状分散体、含水凝胶、水.醇凝胶、醇凝胶或油.醇凝胶、固体棒或气溶胶。
7.一种槲皮素负载香菇菌多糖复合物用于抗炎、抗氧化衰老、护肤的制剂。
8.根据权利要求7所述的应用，其特征在于：所述剂型为临床上可接受的任一剂型。
9.根据权利要求7所述的应用，其特征在于：所述槲皮素负载香菇菌多糖复合物为所述抗炎、抗氧化衰老或护肤的唯一活性成分，或该复合物与其他物质一起制备所述抗炎、抗氧化衰老或护肤的制剂。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910782606.8A CN110638680A (zh) | 2019-08-23 | 2019-08-23 | 一种槲皮素负载香菇菌多糖复合物的制备方法及应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910782606.8A CN110638680A (zh) | 2019-08-23 | 2019-08-23 | 一种槲皮素负载香菇菌多糖复合物的制备方法及应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110638680A true CN110638680A (zh) | 2020-01-03 |
Family
ID=69009817
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910782606.8A Pending CN110638680A (zh) | 2019-08-23 | 2019-08-23 | 一种槲皮素负载香菇菌多糖复合物的制备方法及应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110638680A (zh) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988999A (zh) * | 2012-05-09 | 2013-03-27 | 中国药科大学 | 姜黄素-多糖类偶联物及其制备方法与应用 |
| CN105747232A (zh) * | 2016-03-10 | 2016-07-13 | 无限极（中国）有限公司 | 一种多糖组合物及其应用 |
| CN105919867A (zh) * | 2016-06-01 | 2016-09-07 | 合肥丰瑞隆生物科技有限公司 | 香菇粗多糖护肤品及其制备方法 |
-
2019
- 2019-08-23 CN CN201910782606.8A patent/CN110638680A/zh active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988999A (zh) * | 2012-05-09 | 2013-03-27 | 中国药科大学 | 姜黄素-多糖类偶联物及其制备方法与应用 |
| CN105747232A (zh) * | 2016-03-10 | 2016-07-13 | 无限极（中国）有限公司 | 一种多糖组合物及其应用 |
| CN105919867A (zh) * | 2016-06-01 | 2016-09-07 | 合肥丰瑞隆生物科技有限公司 | 香菇粗多糖护肤品及其制备方法 |
Non-Patent Citations (1)
| Title |
|---|
| 雷敬敷: "香菇发酵工艺及香菇多糖的提取", 《中国食用菌》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20070055373A (ko) | 아토피 피부염 개선제 조성물 | |
| KR20080094461A (ko) | 피부트러블 완화용 화장료 조성물 | |
| JP2023528104A (ja) | 鉄皮石斛オリゴ糖、鉄皮石斛オリゴ糖誘導体及びその調製方法並びに適用 | |
| CN104274355A (zh) | 含有草绿盐角草和芦根的复合提取物的化妆品组合物 | |
| US20180325969A1 (en) | Cosmetic and pharmaceutical applications of lactobacillus pentosus | |
| KR101908678B1 (ko) | 천연 추출물을 함유하는 아토피 피부염 개선용 발효 조성물 | |
| CN108703908B (zh) | 一种抗痘祛斑精华液及其制备方法 | |
| KR20150100288A (ko) | 흑효모로 발효한 생약추출물을 함유하는 화장료 조성물 | |
| KR20070096163A (ko) | 아토피 피부용 조성물 | |
| CN110638829A (zh) | 一种高原儿茶酸负载香菇菌多糖复合物的制备方法及应用 | |
| DE69900547T2 (de) | Kosmetische zusammensetzung, enthaltend eine mischung zur stimulation der interleukin-6 produktion | |
| KR20100013357A (ko) | 녹두 발효-효소 추출액을 포함하는 피부 노화 방지 및 보습용 화장료 조성물 | |
| CN115927128B (zh) | 一种透明颤菌发酵产物及其制备方法与应用 | |
| KR20160143003A (ko) | 화장료 조성물용 아이스플랜트의 유효성분 추출방법 | |
| TW202115241A (zh) | 奇異果發酵汁液以及其用於改善肌膚狀況的用途 | |
| CN110638688A (zh) | 一种青蒿素负载香菇菌多糖复合物的制备方法及应用 | |
| KR20210090870A (ko) | 마이크로바이옴을 이용한 피부 장벽 개선 효과가 우수한 화장료 조성물 | |
| CN110638680A (zh) | 一种槲皮素负载香菇菌多糖复合物的制备方法及应用 | |
| KR20200059446A (ko) | 새싹삼 잎, 줄기 발효액 또는 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물 및 그의 제조방법 | |
| CN110638687A (zh) | 一种丹参素负载香菇菌多糖复合物的制备方法及应用 | |
| KR102196414B1 (ko) | 지의류 유래 미네랄 및 갈락토미세스 복합 발효물을 함유하는 화장료 조성물 | |
| KR101948657B1 (ko) | 톱지네고사리 초음파 추출물을 함유하는 피부 보습용 화장료 조성물 | |
| KR102688754B1 (ko) | 귤꽃 발효추출물의 제조방법 및 이를 함유하는 피부 염증 개선 및 피부 자극 완화 화장료 조성물 | |
| KR20200073584A (ko) | 민들레 및 자소엽 추출물을 유효성분으로 함유하는 여드름 완화용 화장료 조성물 | |
| CN108653149B (zh) | 一种美白保湿面膜及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200103 |
|
| RJ01 | Rejection of invention patent application after publication |