CN110627577A - Compound formula of microbial agent for effectively improving soil and preparation method thereof - Google Patents
Compound formula of microbial agent for effectively improving soil and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a compound formula of a microbial agent for effectively improving soil and a preparation method thereof, wherein the compound formula comprises the following raw materials in parts by weight: 10-15 parts of bacillus subtilis, 6-13 parts of microbial flora, 20-30 parts of volcanic ash, 2-7 parts of gamma-polyglutamic acid water gel, 20-45 parts of straw powder, 20-40 parts of sawdust, 2-5 parts of activated carbon, 7-15 parts of snail shell powder, 10-15 parts of ammonium chloride, 3-13 parts of urea, 10-20 parts of plant ash, 20-40 parts of animal manure, 10-20 parts of bioactive sludge, 2-5 parts of a water-retaining agent, 15-25 parts of enzymolysis biological residue dry powder, 5-8 parts of organic bentonite and 5-13 parts of a decomposition agent. According to the invention, the first microbial agent, the second microbial agent, the microbial flora and the water-retaining agent form the microbial agent, and the first microbial agent, the second microbial agent, the microbial flora and the water-retaining agent are cooperatively acted, so that the microbial agent for soil improvement has a better effect, effectively improves soil hardening, activates soil activity, enables soil to be loose and elastic, forms a soil granular structure, and regulates soil pH value.
Description
Technical Field
The invention relates to the technical field of microbial products, in particular to a compound formula of a microbial agent for effectively improving soil and a preparation method thereof.
Background
Soil is the foundation for our human survival. At present, the wound of the land which we live on is serious, and corresponding measures are required to be taken to repair the soil, such as cultivated land, the appearance of chemical fertilizer is 'lima martensii' for increasing yield, in recent years, blind and excessive chemical fertilizer investment is pursued for yield, the use of organic fertilizer is ignored, the investment cost is increased, the yield is not increased, the soil is seriously damaged, the soil degradation phenomena including low organic matter, soil acidification, secondary salinization, soil hardening and the like occur, and the fertility of the soil is seriously insufficient. Especially for the soil environment of the facility, the soil environment in the facility is worse because the evaporation capacity of the facility is large and no effective precipitation exists, and the fertilizer usage amount is larger for planting horticultural crops with higher economic value in the facility. Therefore, research on soil conditioning technology and reasonable conditioning of facility soil are problems to be solved urgently.
The microorganism has the advantages of improving soil, releasing plant active factors, promoting the growth of plant roots, enhancing the disease resistance of crops, improving the yield and quality of crops, not polluting the environment and the like, is widely applied to the agricultural field in recent years, can accelerate the ion exchange of a soil granular structure through the microbial life activity of the microorganism, further increases the contact rate of the crops and soil organic substances, improves the soil structure, and can open roots after loosening the soil. Secondly, the microorganisms can form metabolites through metabolic activities, so that harmful substances contained in the soil can be effectively absorbed, and a new microecosystem is formed; therefore, the soil hardening can be improved, the soil germs can be reduced, the soil activity can be activated, the soil is loose and elastic, the soil granular structure can be formed, the soil pH value can be adjusted, and the like, which are special effects of microorganisms.
The soil can be hardened after being planted with crops, the pH value of the soil can be changed, the growth of subsequent crops is influenced, and the invention designs the microbial agent for improving the soil aiming at the problem of soil deterioration.
Disclosure of Invention
Solves the technical problem
Aiming at the defects of the prior art, the invention provides a compound formula of a microbial agent for effectively improving soil and a preparation method thereof, and solves the problem that soil is hardened after being used for planting crops, so that the subsequent growth of the crops is influenced.
Technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a compound formula of a microbial agent for effectively improving soil comprises the following raw materials in parts by weight: 10-15 parts of bacillus subtilis, 6-13 parts of microbial flora, 20-30 parts of volcanic ash, 2-7 parts of gamma-polyglutamic acid water gel, 20-45 parts of straw powder, 20-40 parts of sawdust, 2-5 parts of activated carbon, 7-15 parts of snail shell powder, 10-15 parts of ammonium chloride, 3-13 parts of urea, 10-20 parts of plant ash, 20-40 parts of animal manure, 10-20 parts of bioactive sludge, 2-5 parts of a water-retaining agent, 15-25 parts of enzymolysis biological residue dry powder, 5-8 parts of organic bentonite and 5-13 parts of a decomposition agent.
Preferably, the bacillus subtilis comprises bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060, and the mass ratio of the bacillus subtilis KX-1 to the bacillus subtilis KX-2 to the bacillus subtilis KX-4 is 1:0.4-0.85: 0.5-0.85.
Preferably, the water-retaining agent is one of polyacrylamide, sodium polyacrylate and potassium polyacrylate.
Preferably, the microbial flora is one or more of lactobacillus, bacillus natto, photosynthetic bacteria, saccharomycetes and bacillus licheniformis.
Preferably, the mass ratio of the bacillus subtilis KX-1 to the microbial flora is 1: 0.1-0.8.
Preferably, the biological activated sludge is obtained by mixing and biologically treating yeast biological fermentation wastewater and wastewater obtained after glucan is extracted by a yeast cell wall alkaline method, and then filtering, squeezing, drying and crushing the mixture.
Preferably, the enzymatic hydrolysis biological residue dry powder is obtained by homogenizing, biologically hydrolyzing and drying fruit peel residues.
Preferably, the decomposing agent is one of a pig manure fermenting agent and an organic fertilizer fermenting bacterium.
The invention aims to solve another technical problem of providing a preparation method of a composite formula for effectively improving soil by using a microbial agent, which comprises the following steps:
s1, respectively carrying out solid activation culture on bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060 to prepare three bacterial suspensions with the concentrations of 1-3 multiplied by 108 CFU/ml;
s2, inoculating the three bacterial suspensions S1 to a primary seed culture medium respectively according to the inoculation amount with the volume percentage of 3-7%, and culturing to obtain three seed bacterial suspensions with the concentrations of 1-4 multiplied by 1010 CFU/ml;
s3, transferring the three seed bacterial suspensions in the S2 to a fermentation tank for seed culture, adding a water-retaining agent, culturing for 18h at the temperature of 30 ℃, the rotating speed of 120r/min and the ventilation pressure of 0.05MP, and stopping fermentation after the seeds reach the logarithmic phase to obtain a first microbial agent;
s4, mixing the microbial flora and the water-retaining agent in proportion, stirring for 1-2 hours until the mixture is sufficiently and uniformly mixed, and standing for 24 hours at the temperature of 30-35 ℃ to obtain a mixed solution;
s5, weighing volcanic ash, gamma-polyglutamic acid water gel, straw powder, sawdust, activated carbon, snail shell powder, ammonium chloride, urea, plant ash, animal manure, bioactive sludge, enzymatic hydrolysis biological residue dry powder and organic bentonite according to a proportion, stirring for 1-2 hours to be sufficiently and uniformly, adding a decomposition agent during stirring to obtain turbid liquid, and standing for more than 24 hours at 30-35 ℃;
s6, drying the turbid liquid obtained after standing in the step S5 at the temperature of 200 ℃ and 300 ℃ for 0.2-0.3 h, cooling the turbid liquid by a cooling cylinder, and screening the turbid liquid by a screening cylinder to obtain granular materials with the grain size of 1.5-5mm, namely the second microbial agent;
s7, stirring and mixing the second microbial agent in the S6, the first microbial agent in the S3 and the mixed solution in the S4 at the stirring speed of 300-400r/min to finally obtain the composite microbial agent.
Advantageous effects
Compared with the prior art, the invention provides a compound formula of a microbial agent for effectively improving soil and a preparation method thereof, and the compound formula has the following beneficial effects:
the first microbial agent, the second microbial agent, the microbial flora and the water-retaining agent form the microbial agent, the microbial agent for improving the soil has a better effect by cooperation of the first microbial agent, the second microbial agent, the microbial flora and the water-retaining agent, soil hardening is effectively improved, soil activity is activated, the soil is loosened and elastic, a soil granular structure is formed, soil pH value is adjusted, volcanic ash is combined with gamma-polyglutamic acid water gel, the composite formula has very good water retention and water storage effects, the formation of the soil granular structure can be promoted, the microbial environment of plant roots is effectively improved, soil hardening is improved, plant growth is promoted, the quality of crops and forest fruits is improved, the salinization environment of soil in a greenhouse can be improved by straw powder, sawdust, activated carbon, calcium carbonate and spiral shell powder, ammonium chloride and the salinization environment of the soil in the greenhouse can be improved by, The urea, the plant ash and the animal manure can balance nutrients of soil, the organic bentonite promotes the formation of a soil granular structure, improves the internal pore space of the soil, coordinates the proportion of solid, liquid and gas in the soil, promotes the release of quick-acting nutrients, is beneficial to the growth of root systems, and improves the stress resistance of crops.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a compound formula of a microbial agent for effectively improving soil comprises the following raw materials in parts by weight: 13 parts of bacillus subtilis and 3 parts of a water-retaining agent.
Specifically, the bacillus subtilis comprises bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060, and the mass ratio of the bacillus subtilis KX-1 to the bacillus subtilis KX-2 to the bacillus subtilis KX-4 is 1:0.4-0.85: 0.5-0.85.
Specifically, the water-retaining agent is one of polyacrylamide, sodium polyacrylate and potassium polyacrylate.
A preparation method of a compound formula of a microbial agent for effectively improving soil comprises the following steps:
s1, respectively carrying out solid activation culture on bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060 to prepare three bacterial suspensions with the concentrations of 1-3 multiplied by 108 CFU/ml;
s2, inoculating the three bacterial suspensions S1 to a primary seed culture medium respectively in an inoculation amount of 3-7% in volume percentage, and culturing to obtain three seed bacterial suspensions with the concentrations of 1-4 multiplied by 1010 CFU/ml;
s3, transferring the three seed bacterial suspensions in the S2 to a fermentation tank for seed culture, adding 3 parts of water-retaining agent, culturing at the temperature of 30 ℃, the rotating speed of 120r/min and the ventilation pressure of 0.05MP for 18h, and stopping fermentation after the seeds reach the logarithmic phase to obtain the first microbial agent.
Example two:
a compound formula of a microbial agent for effectively improving soil comprises the following raw materials in parts by weight: 25 parts of volcanic ash, 4 parts of gamma-polyglutamic acid hydrogel, 30 parts of straw powder, 30 parts of sawdust, 4 parts of activated carbon, 10 parts of snail shell powder, 13 parts of ammonium chloride, 7 parts of urea, 15 parts of plant ash, 30 parts of animal waste, 15 parts of biological activated sludge, 4 parts of water-retaining agent, 20 parts of enzymolysis biological residue dry powder, 6 parts of organic bentonite and 7 parts of decomposition agent.
Specifically, the biological activated sludge is obtained by mixing and biologically treating yeast biological fermentation wastewater and wastewater obtained after glucan is extracted by a yeast cell wall alkaline method, and then filtering, squeezing, drying and crushing the mixture.
Specifically, the enzymatic hydrolysis biological residue dry powder is obtained by homogenizing, biologically hydrolyzing and drying fruit peel residues.
Specifically, the decomposing agent is one of a pig manure fermenting agent and an organic fertilizer fermenting bacterium.
A preparation method of a compound formula of a microbial agent for effectively improving soil comprises the following steps:
s1, weighing 25 parts of volcanic ash, 4 parts of gamma-polyglutamic acid water gel, 30 parts of straw powder, 30 parts of sawdust, 4 parts of activated carbon, 10 parts of snail shell powder, 13 parts of ammonium chloride, 7 parts of urea, 15 parts of plant ash, 30 parts of animal manure, 15 parts of biological activated sludge, 4 parts of water-retaining agent, 20 parts of enzymolysis biological residue dry powder and 6 parts of organic bentonite according to a proportion, stirring for 1-2 hours to be sufficiently and uniformly, adding 7 parts of decomposition agent during stirring to obtain turbid liquid, and standing for more than 24 hours at 30-35 ℃;
s2, drying the turbid liquid obtained after standing in the S1 at the temperature of 200 ℃ and 300 ℃ for 0.2-0.3 h, cooling the turbid liquid by a cooling cylinder, and screening the turbid liquid by a screening cylinder to obtain granular materials with the grain size of 1.5-5mm, namely the second microbial agent.
Example three:
a compound formula of a microbial agent for effectively improving soil comprises the following raw materials in parts by weight: 15 parts of bacillus subtilis, 13 parts of microbial flora, 30 parts of volcanic ash, 7 parts of gamma-polyglutamic acid water gel, 45 parts of straw powder, 40 parts of sawdust, 5 parts of active carbon, 15 parts of snail shell powder, 15 parts of ammonium chloride, 13 parts of urea, 20 parts of plant ash, 40 parts of animal manure, 20 parts of bioactive sludge, 5 parts of water-retaining agent, 25 parts of enzymolysis biological residue dry powder, 8 parts of organic bentonite and 13 parts of decomposition agent.
Specifically, the bacillus subtilis comprises bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060, and the mass ratio of the bacillus subtilis KX-1 to the bacillus subtilis KX-2 to the bacillus subtilis KX-4 is 1:0.4-0.85: 0.5-0.85.
Specifically, the water-retaining agent is one of polyacrylamide, sodium polyacrylate and potassium polyacrylate.
Specifically, the microorganism flora comprises one or more of lactobacillus, bacillus natto, photosynthetic bacteria, yeast and bacillus licheniformis.
Specifically, the mass ratio of the bacillus subtilis KX-1 to the microbial flora is 1: 0.1-0.8.
Specifically, the biological activated sludge is obtained by mixing and biologically treating yeast biological fermentation wastewater and wastewater obtained after glucan is extracted by a yeast cell wall alkaline method, and then filtering, squeezing, drying and crushing the mixture.
Specifically, the enzymatic hydrolysis biological residue dry powder is obtained by homogenizing, biologically hydrolyzing and drying fruit peel residues.
Specifically, the decomposing agent is one of a pig manure fermenting agent and an organic fertilizer fermenting bacterium.
A preparation method of a compound formula of a microbial agent for effectively improving soil comprises the following steps:
s1, respectively carrying out solid activation culture on bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060 to prepare three bacterial suspensions with the concentrations of 1-3 multiplied by 108 CFU/ml;
s2, inoculating the three bacterial suspensions S1 to a primary seed culture medium respectively in an inoculation amount of 3-7% in volume percentage, and culturing to obtain three seed bacterial suspensions with the concentrations of 1-4 multiplied by 1010 CFU/ml;
s3, transferring the three seed bacterial suspensions in the S2 to a fermentation tank for seed culture, adding 2 parts of water-retaining agent, culturing at the temperature of 30 ℃, the rotating speed of 120r/min and the ventilation pressure of 0.05MP for 18h, and stopping fermentation after the seeds reach the logarithmic phase to obtain a first microbial agent;
s4, mixing the microbial flora 13 with 3 parts of water-retaining agent in proportion, stirring for 1-2 hours until the mixture is sufficiently and uniformly mixed, and standing for 24 hours at 30-35 ℃ to obtain a mixed solution;
s5, weighing 30 parts of volcanic ash, 7 parts of gamma-polyglutamic acid water gel, 45 parts of straw powder, 40 parts of sawdust, 5 parts of activated carbon, 15 parts of snail shell powder, 15 parts of ammonium chloride, 13 parts of urea, 20 parts of plant ash, 40 parts of animal manure, 20 parts of biological activated sludge, 5 parts of water-retaining agent, 25 parts of enzymolysis biological residue dry powder and 8 parts of organic bentonite according to a proportion, stirring for 1-2 hours to be sufficiently and uniformly, adding 13 parts of decomposition agent during stirring to obtain turbid liquid, and standing for more than 24 hours at 30-35 ℃;
s6, drying the turbid liquid obtained after standing in the step S5 at the temperature of 200 ℃ and 300 ℃ for 0.2-0.3 h, cooling the turbid liquid by a cooling cylinder, and screening the turbid liquid by a screening cylinder to obtain granular materials with the grain size of 1.5-5mm, namely the second microbial agent;
s7, stirring and mixing the second microbial agent in the S6, the first microbial agent in the S3 and the mixed solution in the S4 at the stirring speed of 300-400r/min to finally obtain the composite microbial agent.
The following comparative table was obtained by experiment:
| pH value of soil | Degree of soil hardening | Organic matter content of soil | |
| Example one | 8.5 | Height of | 0.2 |
| Example two | 8.3 | Height of | 0.3 |
| EXAMPLE III | 7.2 | Is low in | 0.5 |
The comparison table shows that the effect of the first microbial agent in the first embodiment and the effect of the second microbial agent in the second embodiment are not obvious when the first microbial agent and the second microbial agent are used independently, but the effect achieved by adding the water-retaining agent and the microbial flora is obvious when the first microbial agent and the second microbial agent are mixed, the pH value and the hardness of soil can be improved, the content of organic matters in the soil can be increased, and a good living environment is provided for the growth of crops.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The compound formula of the microbial agent for effectively improving soil is characterized by comprising the following raw materials in parts by weight: 10-15 parts of bacillus subtilis, 6-13 parts of microbial flora, 20-30 parts of volcanic ash, 2-7 parts of gamma-polyglutamic acid water gel, 20-45 parts of straw powder, 20-40 parts of sawdust, 2-5 parts of activated carbon, 7-15 parts of snail shell powder, 10-15 parts of ammonium chloride, 3-13 parts of urea, 10-20 parts of plant ash, 20-40 parts of animal manure, 10-20 parts of bioactive sludge, 2-5 parts of a water-retaining agent, 15-25 parts of enzymolysis biological residue dry powder, 5-8 parts of organic bentonite and 5-13 parts of a decomposition agent.
2. The compound formula of a microbial inoculant for effectively improving soil according to claim 1, wherein the bacillus subtilis comprises bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060, and the mass ratio of the bacillus subtilis KX-1 to the bacillus subtilis KX-2 to the bacillus subtilis KX-4 is 1:0.4-0.85: 0.5-0.85.
3. The compound formulation of claim 1, wherein the water retaining agent is one of polyacrylamide, sodium polyacrylate and potassium polyacrylate.
4. The compound formulation of claim 1, wherein the microbial population is one or more of lactobacillus, bacillus natto, photosynthetic bacteria, yeast, and bacillus licheniformis.
5. The compound formula of a microbial inoculant for effectively improving soil according to claims 1 and 2, wherein the ratio of the bacillus subtilis KX-1 to the microbial flora is 1: 0.1-0.8.
6. The compound formula of a microbial inoculant for effectively improving soil according to claim 1, wherein the bioactive sludge is obtained by mixing and biologically treating yeast biological fermentation wastewater and wastewater obtained by extracting glucan from yeast cell walls by an alkaline method, and then filtering, squeezing, drying and crushing the mixture.
7. The compound formula of a microbial inoculant for effectively improving soil according to claim 1, wherein the enzymatic hydrolysis biological residue dry powder is obtained by homogenizing, biologically hydrolyzing and drying fruit peel residues.
8. The compound formula of a microbial inoculant for effectively improving soil according to claim 1, wherein the decomposing inoculant is one of a pig manure leavening agent and an organic fertilizer zymocyte.
9. A preparation method of a compound formula for effectively improving soil by using a microbial agent is characterized by comprising the following steps:
s1, respectively carrying out solid activation culture on bacillus subtilis KX-1 with a preservation number of CCTCC NoM208057, bacillus subtilis KX-2 with a preservation number of CCTCC NoM208058 and bacillus subtilis KX-4 with a preservation number of CCTCC NoM208060 to prepare three bacterial suspensions with the concentrations of 1-3 multiplied by 108 CFU/ml;
s2, inoculating the three bacterial suspensions S1 to a primary seed culture medium respectively according to the inoculation amount with the volume percentage of 3-7%, and culturing to obtain three seed bacterial suspensions with the concentrations of 1-4 multiplied by 1010 CFU/ml;
s3, transferring the three seed bacterial suspensions in the S2 to a fermentation tank for seed culture, adding a water-retaining agent, culturing for 18h at the temperature of 30 ℃, the rotating speed of 120r/min and the ventilation pressure of 0.05MP, and stopping fermentation after the seeds reach the logarithmic phase to obtain a first microbial agent;
s4, mixing the microbial flora and the water-retaining agent in proportion, stirring for 1-2 hours until the mixture is sufficiently and uniformly mixed, and standing for 24 hours at the temperature of 30-35 ℃ to obtain a mixed solution;
s5, weighing volcanic ash, gamma-polyglutamic acid water gel, straw powder, sawdust, activated carbon, snail shell powder, ammonium chloride, urea, plant ash, animal manure, bioactive sludge, enzymatic hydrolysis biological residue dry powder and organic bentonite according to a proportion, stirring for 1-2 hours to be sufficiently and uniformly, adding a decomposition agent during stirring to obtain turbid liquid, and standing for more than 24 hours at 30-35 ℃;
s6, drying the turbid liquid obtained after standing in the step S5 at the temperature of 200 ℃ and 300 ℃ for 0.2-0.3 h, cooling the turbid liquid by a cooling cylinder, and screening the turbid liquid by a screening cylinder to obtain granular materials with the grain size of 1.5-5mm, namely the second microbial agent;
s7, stirring and mixing the second microbial agent in the S6, the first microbial agent in the S3 and the mixed solution in the S4 at the stirring speed of 300-400r/min to finally obtain the composite microbial agent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363559A (en) * | 2020-04-23 | 2020-07-03 | 罗时举 | Soil environment-friendly restoration agent and preparation method thereof |
| CN113846032A (en) * | 2021-10-13 | 2021-12-28 | 热纳亚环保科技(上海)有限公司 | Preparation method of petroleum hydrocarbon degradation bioflocculant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106701095A (en) * | 2016-12-14 | 2017-05-24 | 郭大磊 | Soil conditioner and application thereof in desertification control |
| CN107879834A (en) * | 2017-10-23 | 2018-04-06 | 柯江波 | A kind of agriculture microbial bacterial agent and its preparation method and application |
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- 2019-03-13 CN CN201910186434.8A patent/CN110627577A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701095A (en) * | 2016-12-14 | 2017-05-24 | 郭大磊 | Soil conditioner and application thereof in desertification control |
| CN107879834A (en) * | 2017-10-23 | 2018-04-06 | 柯江波 | A kind of agriculture microbial bacterial agent and its preparation method and application |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363559A (en) * | 2020-04-23 | 2020-07-03 | 罗时举 | Soil environment-friendly restoration agent and preparation method thereof |
| CN113846032A (en) * | 2021-10-13 | 2021-12-28 | 热纳亚环保科技(上海)有限公司 | Preparation method of petroleum hydrocarbon degradation bioflocculant |
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