CN110623992B

CN110623992B - Preparation and application of folium artemisiae argyi carbon quantum dots (nanoparticles/nanoparticles)

Info

Publication number: CN110623992B
Application number: CN201810658499.3A
Authority: CN
Inventors: 屈会化; 赵琰; 王庆国; 孔慧; 朱雅凡; 张越; 成金俊; 鲁放; 孙紫薇; 张美龄; 闫昕; 曹鹏; 宋兴兴; 罗娟; 熊威; 王苏娜; 王晓克; 侯婷婷
Original assignee: Beijing University of Chinese Medicine
Current assignee: Beijing University of Chinese Medicine
Priority date: 2018-06-22
Filing date: 2018-06-22
Publication date: 2022-03-18
Anticipated expiration: 2038-06-22
Also published as: CN110623992A

Abstract

The invention relates to a method for extracting, separating and purifying quantum dots (nano particles/nano particles) from folium artemisiae argyi charcoal, which comprises the following steps: the folium artemisiae argyi charcoal is extracted or extracted by a solvent, and dialyzed by a dialysis bag with the molecular weight of more than 1000Da or ultrafiltered to obtain folium artemisiae argyi charcoal quantum dots, wherein the quantum dots are blue fluorescence under an ultraviolet lamp of 365nm, have the particle size of less than 100nm measured by a transmission electron microscope and mainly comprise C, H, O, N and other elements; the invention also relates to the application of the quantum dot in the field of medicine, which is used for resisting inflammation, improving the tolerance degree of the body to cold and the like after administration by injection, oral administration or external application and the like.

Description

Preparation and application of folium artemisiae argyi carbon quantum dots (nanoparticles/nanoparticles)

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to preparation of folium artemisiae argyi carbon quantum dots and application of the folium artemisiae argyi carbon quantum dots in the field of medicines.

Background

Since ancient times, methods of treating diseases with charcoal are widely described in medical books of the past dynasties. The pharmacopoeia of the people's republic of China records that it has the functions of warming meridians, stopping bleeding, dispelling cold and relieving pain. The preparation method of the folium artemisiae argyi charcoal mainly comprises the following four methods:

1. direct firing method: cleaning folium Artemisiae Argyi, drying, burning on alcohol burner until dense smoke is formed, burning completely to form cellular loose carbon block, and filtering with Gong or gauze.

2. Pan-frying: cleaning folium Artemisiae Argyi, air drying, parching with slow fire for half an hour until concentrated into mass and dense smoke becomes gradually light into charcoal.

3. A closed-burning method: and (4) collecting folium artemisiae argyi, rinsing with clear water and drying. A small pot or a pot with holes is covered on the pot or the porcelain container. Sealing the joint of the two pots with mud, pressing a weight on the pot, and calcining with slow and strong fire. Generally, the rice or white paper covered on the pot is burnt to be yellow.

4. Baking: placing the folium artemisiae argyi in a drying oven at 275-280 ℃, drying until the thermometer smokes and takes out the folium artemisiae argyi when the folium artemisiae argyi has slight fishy smell.

The folium artemisiae argyi charcoal mainly comprises inorganic elements such as carbon, nitrogen and calcium.

Disclosure of Invention

The quantum dots (nano particles/nano particles) are extracted and separated from the folium artemisiae argyi carbon for the first time, and the folium artemisiae argyi carbon quantum dots are found to have strong biological effects of improving the tolerance degree of animals to cold, resisting inflammation and the like for the first time. The technical scheme is as follows: the invention provides a method for extracting and separating carbon quantum dots from folium artemisiae argyi carbon. The invention also provides the biological activity of the folium artemisiae argyi carbon quantum dots and the application of the folium artemisiae argyi carbon quantum dots in the field of medicines.

The preparation method specifically comprises the following steps: baking to prepare folium Artemisiae Argyi charcoal, extracting quantum dots from folium Artemisiae Argyi charcoal with solvent, and further separating and purifying by dialysis, ultrafiltration, chromatography, etc. to obtain high purity carbon quantum dots.

Drawings

FIG. 1 is a high performance liquid chromatogram of folium Artemisiae Argyi FIG. 1(a) and folium Artemisiae Argyi carbon quantum dot diagram 1(b)

FIG. 2 shows a morphology chart of folium Artemisiae Argyi carbon quantum dot under a transmission electron microscope 2(a), a high resolution transmission electron microscope 2(b), an X-ray diffraction chart 2(c), an ultraviolet spectrum chart 2(d), a fluorescence spectrum chart 2(e), and an infrared spectrum chart 2(f)

FIG. 3 shows the X-ray photoelectron spectrum 3(a), the carbon spectrum 3(b), the oxygen spectrum 3(c), and the nitrogen spectrum 3(d) of the quantum dot of folium Artemisiae Argyi carbon

FIG. 4 shows the effect of different doses of folium Artemisiae Argyi charcoal quantum dots on the concentration of interleukin 1-beta in mouse serum 4(a), the effect on the concentration of tumor necrosis factor in mouse serum 4(b), and the anti-mouse-freezing effect 4 (c).

FIG. 5 is a photograph and a slice of folium artemisiae argyi carbon quantum dots with different dosages for preventing and treating frostbite of mouse ear margins

Detailed Description

Example 1: preparation of folium artemisiae argyi carbon quantum dots

Weighing clean folium Artemisiae Argyi, placing in a crucible, covering, placing in a muffle furnace, heating to 285 deg.C, maintaining constant temperature for 1h, naturally cooling to room temperature, opening the muffle furnace, and taking out to obtain folium Artemisiae Argyi charcoal.

Extracting folium Artemisiae Argyi charcoal with pure water for 3 times, mixing the water solutions, concentrating, and dialyzing with 1KD dialysis bag, wherein the part in the dialysis bag is high-purity folium Artemisiae Argyi charcoal carbon quantum dot solution.

Example 2: preparation of folium artemisiae argyi carbon quantum dots

Weighing clean folium Artemisiae Argyi, placing in a crucible, covering, placing in a muffle furnace, heating to 350 deg.C, maintaining constant temperature for 1h, naturally cooling to room temperature, opening the muffle furnace, and taking out to obtain folium Artemisiae Argyi charcoal.

Extracting folium Artemisiae Argyi charcoal with pure water for 3 times, mixing the water solutions, concentrating, and ultrafiltering with 10KD ultrafilter tube to obtain high purity folium Artemisiae Argyi charcoal carbon quantum dot solution.

Example 3 folium Artemisiae Argyi carbon quantum dots improve the tolerance of the body to cold

Anti-freezing experiment of mice:

1. the night before the experiment was fasted for 12 hours and fed normally. On the day of the experiment, the mice are weighed and divided into physiological saline groups; a group of positive drugs; a high dose group; a medium dose group; low dose group.

2. The labeled mice are separately packaged for later use. The drugs were administered two hours prior to the experiment, respectively, to allow for adequate absorption of the drug. After the holding power of each mouse is measured, the mouse is placed in ice water with the temperature of 0 ℃, is fished out after swimming for 5 minutes, and the holding power is measured again in time, and the relative change value of the holding power is calculated, so that the reaction stiffness degree is obtained.

3. The results are shown in fig. 4, and the three doses, high, medium and low, all have significant anti-freeze effects compared to the normal group (blank group shows that there is still a certain relative change in the mouse's grip without ice-water bath.

The anti-freezing effect of the mouse is as follows:

1. the night before the experiment was fasted for 12 hours and fed normally. The mice were weighed and labeled the day of the experiment.

2. Two hours before the experiment, the mice are subjected to modeling for one week in the ice-water bath method, the mice are killed 12 hours after the last modeling, the serum of the mice is taken to measure inflammatory factors, and the frozen ear margins of the mice are taken to be subjected to HE staining and slicing.

3. The results are shown in fig. 4 and fig. 5, and the high, medium and low doses all have anti-freezing effect compared with the normal group, so that the injury of cold stimulation to mouse tissues can be effectively reduced, and the level of inflammatory factors can be reduced.

Claims

1. The application of the folium artemisiae argyi carbon quantum dots in preparing the medicine for treating frostbite comprises the following steps: placing folium Artemisiae Argyi in a crucible, covering, placing in a muffle furnace, heating to 285 deg.C or 350 deg.C, maintaining constant temperature for 1 hr, naturally cooling to room temperature, and taking out to obtain folium Artemisiae Argyi charcoal; extracting folium Artemisiae Argyi charcoal with pure water, dialyzing with dialysis bag with molecular weight of 1-10 KDa, collecting the fraction retained in the dialysis bag, or ultrafiltering with ultrafiltration tube of 10KD, and collecting the filtrate fraction to obtain folium Artemisiae Argyi charcoal quantum dot; the characteristics of the obtained carbon quantum dots: blue fluorescence under an ultraviolet lamp of 365nm, the particle size measured by a transmission electron microscope is 1-20nm, and the elements of the quantum dots mainly comprise C, O and N elements.

2. The use of claim 1, in the form of injection, oral liquid, hard capsule, soft capsule, tablet, suppository, watered pill, honeyed pill, concentrated pill, paste, powder, emulsion, paste, or liniment.