CN110622893B - Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak - Google Patents
Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak Download PDFInfo
- Publication number
- CN110622893B CN110622893B CN201911072150.2A CN201911072150A CN110622893B CN 110622893 B CN110622893 B CN 110622893B CN 201911072150 A CN201911072150 A CN 201911072150A CN 110622893 B CN110622893 B CN 110622893B
- Authority
- CN
- China
- Prior art keywords
- eggs
- saprolegniasis
- hatching
- disinfection
- flowing water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 42
- 206010039710 Scleroderma Diseases 0.000 title description 2
- 235000013601 eggs Nutrition 0.000 claims abstract description 103
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 78
- 241000876437 Brachymystax lenok Species 0.000 claims abstract description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 94
- 238000011534 incubation Methods 0.000 claims description 49
- 238000004659 sterilization and disinfection Methods 0.000 claims description 37
- 238000005273 aeration Methods 0.000 claims description 12
- 230000004720 fertilization Effects 0.000 claims description 9
- 238000006213 oxygenation reaction Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 239000004575 stone Substances 0.000 claims description 2
- 241000233667 Saprolegnia Species 0.000 abstract description 8
- 210000002257 embryonic structure Anatomy 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000004763 spore germination Effects 0.000 abstract description 3
- 230000000638 stimulation Effects 0.000 abstract description 3
- 208000035404 Autolysis Diseases 0.000 abstract description 2
- 206010057248 Cell death Diseases 0.000 abstract description 2
- 230000028043 self proteolysis Effects 0.000 abstract description 2
- 241000251468 Actinopterygii Species 0.000 description 14
- 235000019688 fish Nutrition 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 7
- 230000003203 everyday effect Effects 0.000 description 7
- 230000001706 oxygenating effect Effects 0.000 description 7
- 230000000249 desinfective effect Effects 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 241000219146 Gossypium Species 0.000 description 2
- 241001417902 Mallotus villosus Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008143 early embryonic development Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000251556 Chordata Species 0.000 description 1
- 241000251464 Coelacanthiformes Species 0.000 description 1
- 208000007163 Dermatomycoses Diseases 0.000 description 1
- 244000299507 Gossypium hirsutum Species 0.000 description 1
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- 208000025309 Hair disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 241000277299 Salmoniformes Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 201000003929 dermatomycosis Diseases 0.000 description 1
- 208000034653 disorder of pilosebaceous unit Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008832 photodamage Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention provides a brachymystax lenok egg hatching method for preventing saprolegniasis outbreak, wherein in the early stage of brachymystax lenok egg hatching, low-concentration formalin is used for inhibiting saprolegnia spore germination and delaying saprolegniasis; after saprolegniasis occurs, the mycelium of the saprolegniasis is quickly killed by high-concentration formalin, so that the spread of the saprolegniasis is controlled; before autolysis of dead eggs and at the stage of insensitivity to external stimulation in the development of brachymystax lenok embryos, the dead eggs are manually removed in time, and the large outbreak of saprolegniasis caused by conglutination of the dead eggs and live eggs is prevented; after the dead eggs are manually removed, the recurrence of saprolegniasis is prevented by high-concentration formalin. By the method, large-area saprolegniasis outbreaks at the hatching stage of the brachymystax lenok eggs can be effectively prevented, the average hatching rate can be improved to more than 92.6%, and meanwhile, the method is simple, convenient, feasible and low in cost, is suitable for being applied to large-scale breeding production, and has high popularization and application values.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak.
Background
Lepidote fish (Brachymystax lenok) is a member of the phylum chordata, the subphylum vertebrates, the class Osteichthyes, the class Radiata, the order Salmoniformes, the family Salmonidae, the genus Lepidote fish. The capelin is one of the special fishes in alpine regions in China, only one fish belongs to the world, the capelin has rich meat flavor, high fat content (3.8-7.7 percent), rich nutrition and rare eggs, and is a rare economic fish. In recent years, due to the destruction of natural ecological environment and the interference of human factors, the resource quantity of the brachymystax lenok is reduced year by year and is listed as a national secondary protection animal. Therefore, the method is particularly important for vigorously developing the resource protection, proliferation and culture of the brachymystax lenok, and meanwhile, the abundant cold water resources in alpine regions of China are fully utilized to carry out the scale-increasing culture of the brachymystax lenok, so that the living standard of people is continuously improved, greater economic and social benefits are created, and the method has important significance for the sustainable development of the scale-increasing culture of the cold water fishery of China.
Saprolegniasis (Saprolegniasis) is one of the common fungal diseases of aquatic fishes, and is also called covering cotton disease, gossypium hirsutum disease, dermatomycosis or white hair disease because the wounded tissues and dead eggs on the body surfaces of diseased fishes are often infected by pathogens such as Saprolegniasis or cotton mold to form a gray-white flocculent covering. Almost all fishes and eggs can be infected all the year round, and the spread is rapid, which brings great economic loss to the aquaculture industry. The brachymystax lenok is a typical cold water fish, the optimum hatching temperature of eggs is 6-12 ℃, the brachymystax lenok is very suitable for growth and propagation of saprolegnia, the hatching period is long and is generally about 20 days, therefore, saprolegnia can hardly be thoroughly avoided in actual production, dead eggs are easy to be adhered with live eggs to form lumps, the live eggs are anoxic, and the development of the saprolegnia is promoted. Thus, once saprolegniasis occurs, it causes an immeasurable loss. The brachymystax lenok is sensitive to light and vibration in the early embryonic development stage, particularly the embryo hole closing stage, so that a method for manually picking out dead eggs in the early embryonic development stage is not feasible, and saprolegniasis must be prevented by other methods.
Disclosure of Invention
In order to solve the technical problem, the invention provides a method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak.
The specific technical scheme of the invention is as follows:
the invention provides a method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak, which comprises the following steps:
s1: from the 2 nd day of fertilization, low-frequency disinfection is carried out by adopting low-concentration formalin, and aeration and oxygenation are continuously carried out in the period;
s2: after saprolegniasis occurs, high-concentration formalin is used for high-frequency disinfection, and aeration is continuously carried out during the disinfection;
s3: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s4: after dead eggs are removed, low-frequency disinfection is carried out by adopting high-concentration formalin, and aeration and oxygenation are continuously carried out until hatching is finished.
The brachymystax lenok embryo is sensitive to external stimulation (light, vibration and the like) in the gastral period and the embryo hole closing period, so that formalin is only used for prevention and treatment in the early hatching period. The low-concentration formalin is used for preventing in the early stage of incubation, thus inhibiting saprolegnia spore germination, reducing the probability of saprolegnia and delaying the saprolegnia occurrence time. After saprolegniasis occurs, high-concentration and low-concentration formalin is used for treatment, hyphae of saprolegniasis can be killed, and saprolegniasis spread can be effectively controlled. When the brachymystax lenok eggs are incubated for more than 10 days, the dead eggs are gradually dissolved and the cytoplasm flows out, and the dead eggs and the live eggs are adhered to each other to form an egg mass, so that the live eggs are partially anoxic and die, the formalin is difficult to reach the inside of the egg mass, the saprolegniasis is caused to rapidly burst, and in severe cases, all fertilized eggs can die, so that the dead eggs need to be removed in time. In the process of manually removing dead eggs, the brachymystax lenok embryos are easily injured and even die, so that the dead eggs are removed when the incubation accumulated temperature reaches 100 ℃ d and the sensitivity of the brachymystax lenok embryos to external stimuli is reduced, the probability of injury of the brachymystax lenok embryos is reduced, the hatching rate is remarkably improved, meanwhile, manual egg picking is not performed after the dead eggs are manually removed once, and high-concentration formalin is used for controlling and preventing saprolegniasis outbreak.
Further, the concentration of the low-concentration formalin is 100-200 ml/m3The concentration of the high-concentration formalin is 300-400 ml/m3。
Further, the frequency of low-frequency disinfection is 1 time per day, and the frequency of high-frequency disinfection is 2-3 times per day.
Further, in the hatching process, micro flowing water is adopted for hatching, and the flowing water is stopped for 1h during disinfection.
Furthermore, the flow velocity of the micro-flow water is 8-10L/min, and the water exchange rate is 50-60%/h.
Furthermore, the aeration and oxygenation are micro aeration or microwave aeration, and the air stone avoids the hatching basket to avoid vibrating the fish eggs.
Further, during incubation, fertilized eggs are paved and aged for 2-3 layers in an incubation container.
Further, the incubation temperature is 6-12 ℃.
The invention has the following beneficial effects: the invention provides a brachymystax lenok egg hatching method for preventing saprolegniasis outbreak, wherein in the early stage of brachymystax lenok egg hatching, low-concentration formalin is used for inhibiting saprolegnia spore germination and delaying saprolegniasis; after saprolegniasis occurs, the mycelium of the saprolegniasis is quickly killed by high-concentration formalin, so that the spread of the saprolegniasis is controlled; before autolysis of dead eggs and at the stage of insensitivity to external stimulation in the development of brachymystax lenok embryos, the dead eggs are manually removed in time, and the large outbreak of saprolegniasis caused by conglutination of the dead eggs and live eggs is prevented; after the dead eggs are manually removed, the recurrence of saprolegniasis is prevented by high-concentration formalin. By the method, large-area saprolegniasis outbreaks at the hatching stage of the brachymystax lenok eggs can be effectively prevented, the average hatching rate can be improved to more than 92.6%, and meanwhile, the method is simple, convenient, feasible and low in cost, is suitable for being applied to large-scale breeding production, and has high popularization and application values.
Detailed Description
The present invention will be described in further detail with reference to the following examples.
Example 1
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 6 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: from day 2 of fertilization, 100ml/m was administered 1 time per day3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 300ml/m is used instead3Disinfecting formalin in a water body 3 times every day, pausing flowing water for 1h during disinfection, and then recovering micro flowing water;
s4: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s5: after dead eggs were removed, 300ml/m was used 1 time a day3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Example 2
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 10 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 150ml/m was administered 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 350ml/m is used instead3Disinfecting formalin in a water body 3 times every day, pausing flowing water for 1h during disinfection, and then recovering micro flowing water;
s4: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s5: after dead eggs were removed, 350ml/m was used 1 time a day3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Example 3
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 12 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 200ml/m was used 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 400ml/m is used instead3Formalin in water is disinfected for 2 times every day, flowing water is suspended for 1h during disinfection, and then micro-flowing water is recovered;
s4: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s5: after removing dead eggs, 400ml/m was used 1 time a day3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Comparative example 1
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 10 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 150ml/m was administered 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 350ml/m is used instead3Disinfecting formalin in a water body 3 times every day, pausing flowing water for 1h during disinfection, and then recovering micro flowing water;
s4: after the incubation accumulated temperature reaches 100 ℃ d, the temperature is adopted for 1 time every day at 350ml/m3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Comparative example 2
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 10 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 150ml/m was administered 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 350ml/m is used instead3Disinfecting formalin in a water body 3 times every day, pausing flowing water for 1h during disinfection, and then recovering micro flowing water;
s4: when the incubation accumulated temperature reaches 100 ℃ d, the dead eggs are manually removed, and then the incubation with microflow water is continued until the incubation is finished.
Comparative example 3
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 10 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 200ml/m was used 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: after saprolegniasis occurs, 400ml/m is used instead3Formalin in water is disinfected for 2 times every day, flowing water is suspended for 1h during disinfection, and then micro-flowing water is recovered;
s4: when the incubation accumulated temperature reaches 80 ℃ d, manually removing dead eggs;
s5: after removing dead eggs, 40 times daily for 1 time0ml/m3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Comparative example 4
A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak comprises the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, incubating the brachymystax lenok eggs by adopting incubation conditions of micro-flowing water (the flow rate is 8-10L/min, the water exchange rate is 50-60%/h) and 10 ℃, and continuously ventilating and oxygenating during the whole incubation period;
s2: 200ml/m was used 1 time a day from day 2 of fertilization3Disinfecting formalin in a water body, suspending flowing water for 1h during disinfection, and then recovering micro-flowing water;
s3: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s4: after dead eggs were removed, 350ml/m was used 1 time a day3And (3) disinfecting formalin in the water body, suspending flowing water for 1h during disinfection, and then recovering micro flowing water until hatching is finished.
Laboratory mini-incubation test
Taking 8 identical small-size fish egg hatching containers, and respectively spreading two layers of brachymystax lenok eggs from the same source at the bottoms, wherein T1-T7 are hatched by adopting the methods provided in experimental examples 1-3 and comparative examples 1-4 respectively; t8 is a blank control incubated at the same temperature, water flow rate, and aeration flow rate as the other groups, without any other treatment. After the incubation is finished, the hatchability of each group is counted respectively.
TABLE 1 comparison of incubation results by different methods
As can be seen from Table 1, the hatchability of each group T1-T3 is more than 90% and is significantly higher than that of the rest groups, and the hatchability is less than 2.5% and is significantly lower than that of the control groups 1, 3 and 4, which shows that the hatchability of the brachymystax lenok eggs can be significantly improved and the hatchability of the fry can be effectively reduced by applying the method provided by the application. T4 does not carry out the operation of manually removing dead eggs, which causes saprolegniasis outbreak at the late stage of hatching, and finally seriously influences the rate of emergence and has higher distortion rate; t5 does not carry out disinfection treatment after the dead eggs are removed manually, which causes saprolegniasis to spread to a certain extent but not to explode in a large range, and the fry is developed completely and has a light damage degree, the emergence rate is reduced to a certain extent, but the deformity rate is not influenced obviously; t6 advances the time of removing dead eggs manually, at the moment, the fry is not fully developed, so that the fish eggs are damaged to a certain extent, the emergence rate is obviously reduced, and the deformity rate is increased to a certain extent; the dosage of formalin is not increased when the T7 is subjected to saprolegniasis, so that the treatment effect is not obvious, and the emergence rate and the deformity rate are seriously influenced; the blank control was left without any action, resulting in a large outbreak of saprolegniasis, and eventually all roe died. Therefore, the method provided by the application can effectively prevent the outbreak of saprolegniasis in a small hatching environment of a laboratory, can obviously improve the hatching rate of the brachymystax lenok eggs, and effectively reduces the fry distortion rate.
Examples of the applications
Selecting a plurality of cold water fish farms such as baoding Changyuan fishery, baoding Quyang Shengshui Bay aquatic product culture limited company, Qinglongtazhan cold water fish culture science and technology demonstration garden, and Bingfeizin aquaculture limited company in 2015-2018, performing a small scale salmon egg hatching test by adopting the method provided by the application, simultaneously setting a control group applying the hatching methods provided by the comparative example 1 and the comparative example 2 at each test site respectively, setting a blank control without any measures, and comparing the hatching rates of different groups of the farms.
The results show that when the method provided by the application is used for hatching, large-area outbreak of saprolegniasis does not occur in each farm, the hatching rates of the brachymystax lenok eggs in each farm are not greatly different and are all between 85.3% and 96.7%, and the average hatching rate is 92.6%; all brachymystax lenok eggs of the blank control groups of all households die due to saprolegniasis outbreak; when the incubation accumulated temperature reaches 100 ℃ d, a control group without manually removing dead eggs loses seriously due to the fact that saprolegniasis is difficult to control in the late incubation period, the incubation rate fluctuates within the range of 22.3-43.4%, and the average incubation rate is only 31.6%; after the incubation accumulated temperature reaches 100 ℃ d, only removing the control group of dead eggs by a manual method, wherein the incubation rate of the brachymystax lenok eggs is 75.4-81.7%, and the average incubation rate is 77.9%; the above results are also consistent with the rules of laboratory mini-hatching experiments. By comparison, the effect of treating the oosaprolegniasis of the brachymystax lenok by adopting the scheme is best for improving the hatching rate of the brachymystax lenok. Therefore, the method provided by the application can be widely applied to large-scale artificial breeding of the brachymystax lenok.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak is characterized by comprising the following steps:
s1: paving 2-3 layers of fertilized eggs in an incubation container, and incubating the brachymystax lenok eggs by adopting microflow water with the flow rate of 8-10L/min and the water exchange rate of 50-60%/h under the incubation condition of 6-12 ℃; from the 2 nd day of fertilization, low-frequency disinfection is carried out by adopting low-concentration formalin, aeration and oxygenation are continuously carried out in the process, flowing water is temporarily stopped for 1 hour during disinfection, and then micro-flowing water is recovered;
s2: after saprolegniasis occurs, high-concentration formalin is used for high-frequency disinfection, aeration and oxygenation are continuously carried out in the process, flowing water is temporarily stopped for 1 hour during disinfection, and then micro-flowing water is recovered;
s3: when the incubation accumulated temperature reaches 100 ℃ d, manually removing dead eggs;
s4: after dead eggs are removed, high-concentration formalin is adopted for low-frequency disinfection, air is continuously filled for oxygenation in the disinfection period, flowing water is temporarily stopped for 1 hour during disinfection, and then micro flowing water is recovered until hatching is finished;
the concentration of the low-concentration formalin is 100-200 ml/m3The concentration of the high-concentration formalin is 300-400 ml/m3；
The low-frequency disinfection frequency is 1 time per day, and the high-frequency disinfection frequency is 2-3 times per day.
2. The method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak according to claim 1, wherein the hatching process is carried out by using running water, and the running water is stopped for 1 hour during the disinfection.
3. The method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak according to claim 2, wherein the flow rate of said micro-flowing water is 8 to 10L/min, and the water exchange rate is 50 to 60%/h.
4. The method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak according to claim 1, wherein said aeration is micro-aeration or microwave aeration and air stones are kept away from the hatching basket to avoid vibrating the eggs.
5. The method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak according to claim 1, wherein in hatching, fertilized eggs are laid in 2-3 layers in a hatching container.
6. The method for hatching brachymystax lenok eggs for preventing saprolegniasis outbreak according to any one of claims 1 to 5, wherein the hatching temperature is 6 to 12 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911072150.2A CN110622893B (en) | 2019-11-05 | 2019-11-05 | Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911072150.2A CN110622893B (en) | 2019-11-05 | 2019-11-05 | Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110622893A CN110622893A (en) | 2019-12-31 |
| CN110622893B true CN110622893B (en) | 2021-11-30 |
Family
ID=68979269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911072150.2A Expired - Fee Related CN110622893B (en) | 2019-11-05 | 2019-11-05 | Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110622893B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050083219A (en) * | 2004-02-05 | 2005-08-26 | 강원도 화천군 | Smoked-salmon manufacturing method and smoked-salmon |
| CN102783442A (en) * | 2012-08-23 | 2012-11-21 | 甘肃省水产研究所 | Artificial breeding method of Gymnodiptychus pachycheilus |
| WO2016063432A1 (en) * | 2014-10-21 | 2016-04-28 | 助川化学株式会社 | Method for controlling saprolegniasis in culture water |
| CN106688983A (en) * | 2016-11-21 | 2017-05-24 | 四川省农业科学院水产研究所 | Prevention and treatment method of saprolegniasis in incubation period of hucho bleekeri fertilized eggs |
| CN107278994A (en) * | 2017-06-13 | 2017-10-24 | 中国水产科学研究院黑龙江水产研究所 | One kind prevention fish oosperm saprolegniasis method |
| CN107320581A (en) * | 2017-07-20 | 2017-11-07 | 通化师范学院 | It is a kind of to prevent Chinese herbal feed additive of brachymystax lenok bacillary enteritis and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5868998A (en) * | 1991-06-26 | 1999-02-09 | Arbor Acres Farm, Inc. | Method for the microaerosol fumigation of newly hatched poultry |
| CN105555131B (en) * | 2014-07-31 | 2017-09-01 | 大峰Ls株式会社 | Continuous-flow type aqua sterilisa method for cultivation of fish and continuous-flow type aqua sterilisa fish cultivating system |
-
2019
- 2019-11-05 CN CN201911072150.2A patent/CN110622893B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050083219A (en) * | 2004-02-05 | 2005-08-26 | 강원도 화천군 | Smoked-salmon manufacturing method and smoked-salmon |
| CN102783442A (en) * | 2012-08-23 | 2012-11-21 | 甘肃省水产研究所 | Artificial breeding method of Gymnodiptychus pachycheilus |
| WO2016063432A1 (en) * | 2014-10-21 | 2016-04-28 | 助川化学株式会社 | Method for controlling saprolegniasis in culture water |
| CN106688983A (en) * | 2016-11-21 | 2017-05-24 | 四川省农业科学院水产研究所 | Prevention and treatment method of saprolegniasis in incubation period of hucho bleekeri fertilized eggs |
| CN107278994A (en) * | 2017-06-13 | 2017-10-24 | 中国水产科学研究院黑龙江水产研究所 | One kind prevention fish oosperm saprolegniasis method |
| CN107320581A (en) * | 2017-07-20 | 2017-11-07 | 通化师范学院 | It is a kind of to prevent Chinese herbal feed additive of brachymystax lenok bacillary enteritis and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 秦岭细鳞鲑人工繁殖及鱼苗培育技术;李勤慎 等;《中国水产》;20130430;第55-56页 * |
| 秦岭细鳞鲑早期发育观察;施德亮 等;《中国水产科学》;20120731;第557-565页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110622893A (en) | 2019-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103141426B (en) | Method for efficiently breeding blue crabs | |
| TWI398219B (en) | A use of sodium alginate in promoting breeding ability of geneus penaeus | |
| CN104082253B (en) | Leech and snail, pasture and water commensalism enclosure fence high-yield cultivation method | |
| CN102487866B (en) | Large-scale natural propagation method for artificially culturing siganus guttatus parents | |
| CN107372231B (en) | A kind of disease Synthetical prevention method improving Farmed as Main Species in Ponds grass carp survival rate | |
| CN104256172A (en) | Odontobutis obscura parent fish feed and preparation method thereof | |
| CN106417122B (en) | A kind of breeding method improving high-quality freshwater shrimp seed yield | |
| CN103548736B (en) | The domestication by low temperature breeding method of a kind of Huso huso seed | |
| CN103141411B (en) | Hippocampus erectus parent sea horse pairing method | |
| CN107667936A (en) | One seed lotus set supports lobster, the ecological cultivation method of soft-shelled turtle | |
| CN105557590B (en) | A kind of Australia freshwater lobster in-vitro hatching method | |
| CN110622893B (en) | Scleroderma parvum egg hatching method for preventing saprolegniasis outbreak | |
| CN107027697A (en) | A kind of method for improving free range chicken hatching of breeding eggs rate | |
| CN105994039B (en) | A kind of intensive culture method improving long Qi Wen Minnow seed rearing survival rates | |
| CN107347742A (en) | A kind of method of efficiently culturing rice field eel | |
| CN107098483A (en) | A kind of improver of water quality | |
| CN106172098A (en) | The high-yield cultivation method of white shrimp | |
| CN103598124B (en) | The method of the popular ulcer syndrome of a kind of bionomic control fish | |
| CN104813914B (en) | The prevention and controls of pest and disease damage in the culture of algae germling | |
| CN103858800A (en) | Culture method of river prawns | |
| CN109699546A (en) | A kind of high-yield and high-efficiency cultural method of river crab | |
| CN108743930A (en) | The preparation method of Fugu rubripes Uronema marinum disease vaccine | |
| CN106614186A (en) | Indoor small water body cultivating method for South American white shrimps at temporary cultivation stage for thickening | |
| CN106396120A (en) | American frog culture wastewater treatment method | |
| CN116746521A (en) | Method for preventing and treating sea horse melon insect disease with abdomen expansion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211130 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |