CN110612355A - 用于定量pcr扩增的组合物及其应用 - Google Patents

用于定量pcr扩增的组合物及其应用 Download PDF

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CN110612355A
CN110612355A CN201780090491.5A CN201780090491A CN110612355A CN 110612355 A CN110612355 A CN 110612355A CN 201780090491 A CN201780090491 A CN 201780090491A CN 110612355 A CN110612355 A CN 110612355A
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primer
sequence
specific
amplification
composition
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CN110612355B (zh
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杨林
张薇婷
高雅
张艳艳
陈芳
夏军
蒋慧
徐讯
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MGI Tech Co Ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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Abstract

提供了用于定量PCR扩增的组合物，其包括：一对PCR引物对和一条特异性探针，所述PCR引物对包括第一引物和第二引物，其中，所述第一引物包含第一特异性序列和第一随机序列，所述第一特异性序列位于所述第一引物的3’端，所述第一随机序列位于所述第一引物的5’端，所述第二引物包含第二特异性序列和第二随机序列，所述第二特异性序列位于所述第二引物的3’端，所述第二随机序列位于所述第二引物的5’端，并且，所述第一特异性序列和所述第二特异性序列分别为针对靶序列的上游引物和下游引物，所述第一随机序列和所述第二随机序列反向互补，所述特异性探针的序列与所述靶序列的上游引物和下游引物之间的序列互补配对。

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PCT国内申请，说明书已公开。

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  1. PCT国内申请，权利要求书已公开。
CN201780090491.5A 2017-06-20 2017-06-20 用于定量pcr扩增的组合物及其应用 Active CN110612355B (zh)

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CA3140403A1 (en) * 2019-06-26 2020-12-30 Mgi Tech Co., Ltd. Method for preparing nested multiplex pcr high-throughput sequencing library and kit

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AU1207899A (en) * 1997-10-28 1999-05-17 Regents Of The University Of California, The Dna polymorphism identity determination using flow cytometry
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WO2016161054A1 (en) * 2015-04-01 2016-10-06 Pharmacyclics Llc Massive parallel primer dimer-mediated multiplexed single cell-based amplification for concurrent evaluation of multiple target sequences in complex cell mixtures
CN105624309A (zh) * 2016-02-23 2016-06-01 深圳华大基因研究院 检测EGFR和/或K-ras基因突变的引物、探针及试剂盒

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