CN110604225A - Pig intestinal health regulator and preparation method thereof - Google Patents

Pig intestinal health regulator and preparation method thereof Download PDF

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CN110604225A
CN110604225A CN201810619106.8A CN201810619106A CN110604225A CN 110604225 A CN110604225 A CN 110604225A CN 201810619106 A CN201810619106 A CN 201810619106A CN 110604225 A CN110604225 A CN 110604225A
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parts
vitamin
intestinal health
extract
acid
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孙育荣
王燕
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NANJING AOMAI SCI & TECH Co
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to the technical field of pig feed, and particularly relates to a pig intestinal health regulator and a preparation method thereof, wherein the regulator comprises the following components in parts by weight: 50-80 parts of acidifier, 20-50 parts of plant extract, 1-3 parts of probiotics, 1-5 parts of saccharomyces cerevisiae extract, 1-3 parts of composite Chinese herbal medicine, 0.5-1.5 parts of glutamine and 15-35 parts of coated composite vitamin. The regulator prepared by the invention can replace antibiotics to be used in pig feed, prevent pig diarrhea and promote pig growth.

Description

Pig intestinal health regulator and preparation method thereof
Technical Field
The invention belongs to the technical field of pig feed, and particularly relates to a pig intestinal health regulator and a preparation method thereof.
Background
Feed antibiotics include antibiotic growth promoters and therapeutic antibiotics. The former is an antibiotic which is widely used in the feeding of edible animals and can improve the production benefit of the animals; the latter is mainly used for therapy, i.e. the feed used by the animal in the diseased state, to which some antibiotics can be added in the case of veterinary prescriptions.
Antibiotics were added as growth promoters to feed, beginning in the 50's of the 20 th century, with the aim of promoting growth, improving feed utilization, reducing mortality and improving reproductive performance. In 1950, antibiotics are allowed to be added into feed, and are beginning to be widely used as growth promoters in livestock and poultry breeding.
For a long time, the antibiotic growth promoter has good effect when applied to the animal husbandry production, is regarded as the largest biotechnology of the 21 st century livestock breeding, and greatly promotes the development of the livestock breeding industry and the feed industry. Approximately 50% of the total antibiotic production worldwide was used in animal husbandry in 1996, but due to the continuous low-level feeding of antibiotics, the hazards of bacterial drug resistance, drug residues in animal products, anaphylactic toxic reactions, and "triple effects" (mutagenicity, carcinogenesis and teratogenesis) are becoming more and more significant. The occurrence of human drug resistance events directly threatens human health and safety, causes consumer panic, and forces livestock and poultry breeding to change production modes.
In 22.6.2017, the ministry of agriculture formally establishes a national plan for suppressing animal-derived bacteria drug resistance activities (2020 2017), wherein 6 important tasks are specified, the first task is the exit of growth-promoting antibacterial drugs, which is divided into three categories: the first type is a common antibacterial drug for promoting growth of human and animals, such as a chlortetracycline premix, which is required to be cleaned; the second type is a special antibacterial drug for growth-promoting animals, such as a flavomycin premix, and policy suggestions for reservation or withdrawal need to be formed; the third type is quinoxaline antibacterial drugs, such as mequindox, olaquindox, quinocetone and the like, and a gradual exit scheme is formed.
Nowadays, olaquindox, organic arsenic and other drug feed additives are forbidden, and in the future, more and more 168 bulletins will be provided to stipulate that the currently temporarily allowed "drug-added" products will be forbidden, so that the health and immunity of animals need to be improved from the aspects of feeding management, nutrition level, environmental control and the like, and the use of growth-promoting drug feed additives is gradually reduced.
The main effects of antibiotics on animal body are bacteriostasis and growth promotion. At present, a plurality of products with antibacterial and growth promoting effects are available at home and abroad, and comprise microecologics, antibacterial peptides, organic acids, polysaccharide/oligosaccharide, plant extracts, plant essential oil, Chinese herbal medicines, novel enzyme preparations and the like. Although there are many products available for user selection, there are few and few that can truly provide customers with a comprehensive antibiotic alternative.
The feeding acidifier is a novel additive capable of reducing the pH value of the feed in the digestive tract and providing the most suitable digestive tract environment for animals, and has a single type and a compound type. The acid component includes inorganic acid and organic acid. But the antibacterial agent has a better effect only on piglets in the nursery stage, and has the problems of large addition amount, corrosion on production equipment and the like although the antibacterial effect is obvious. The enzyme is a catalyst for various chemical reactions in the metabolism process in the organism, and has specificity and high efficiency. The enzyme preparation added into the feed is a product with biological activity produced by fermentation of various microorganisms. The enzyme preparation mainly comprises digestive enzymes such as protease, amylase and cellulose powder enzyme; and enzymes for improving feed quality, such as xylanase, pentosanase, pectinase and phytase. The enzyme preparation can improve the feed conversion rate, but has poor bacteriostatic effect. The plant extract is a research hotspot in recent years, and the types of the components of the plant extract are very many, and mainly comprise various types such as flavonoids, alkaloids, glycosides, quinones, phenylpropanoids, tannins, lipids, volatile oils and the like. The effects in animal bodies are mainly antibacterial, bactericidal, antioxidant and the like. But simultaneously, a plurality of plant extracts have the defects of easy volatilization, easy oxidation, undefined active ingredients and difficult processing and production.
From the results of current experimental studies, it is difficult to completely replace antibiotics with a single product to achieve the anti-diarrhea and growth-promoting effects, and the problem must be solved through comprehensive scheme proportioning.
Disclosure of Invention
The invention mainly provides a pig intestinal health regulator and a preparation method thereof, and the regulator can be used in pig feed instead of antibiotics to prevent pig diarrhea and promote pig growth. The technical scheme is as follows:
an intestinal health regulator for pigs comprises the following components in parts by weight: 50-80 parts of acidifier, 20-50 parts of plant extract, 1-3 parts of probiotics, 1-5 parts of saccharomyces cerevisiae extract, 1-3 parts of composite Chinese herbal medicine, 0.5-1.5 parts of glutamine and 15-35 parts of coated composite vitamin.
Preferably, the acidifying agent is selected from one or more of formic acid, acetic acid, butyric acid, citric acid, fumaric acid, sodium diacetate and potassium sorbate, and is mixed with a silicon dioxide carrier.
Preferably, the plant extract is one or more of sanguinarine extracted from macleaya cordata, chlorogenic acid extracted from green coffee, liquiritigenin, ginkgetin, cinnamaldehyde, carvacrol and eugenol.
Preferably, the probiotics are at least two of clostridium butyricum, lactobacillus and bacillus subtilis.
Preferably, the compound Chinese herbal medicine is a mixture of campanumoea root, hawthorn, Chinese date and dried orange peel.
Preferably, the core material coated with the compound vitamin comprises vitamin A and vitamin D3Vitamin E, vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12The wall material coated with the compound vitamin comprises one or more of vegetable wax, animal wax and gelatin.
A preparation method of the pig intestinal health regulator comprises the following steps: mixing 70-80% of the formula amount of coated multivitamin with the formula amount of acidifier, probiotics, plant extract, Saccharomyces cerevisiae extract, compound Chinese herbal medicine and glutamine, mixing well, adding the rest coated multivitamin, and mixing well to obtain the compound feed additive.
By adopting the scheme, the invention has the following advantages:
the product is designed aiming at the characteristics of the gastrointestinal tract of pigs, particularly weaned piglets, and consists of an acidifier and a plant extract, wherein the acidifier has the effects of improving the palatability of piglet feed, enhancing the digestion function of the weaned piglets, preventing and controlling animal diarrhea and promoting the production performance of the piglets. The plant extract has antibacterial effect and can increase feed intake.
Detailed Description
In recent years, a great deal of research proves that acidifiers and plant extract products have diarrhea-resisting and growth-promoting effects similar to antibiotics and can replace antibiotics. The acidifier and plant extract compound product can be used as a novel green feed additive, is expected to become an antibiotic substitute for feed enterprises in the course of non-antibiotic feed reformation, and has good market space and prospect.
(1) According to the action mechanism and the effect of the acidifier, the stomach digestive acid and the intestinal acid are reasonably selected to achieve the effects of improving the palatability of the piglet feed, enhancing the digestive function of the weaned piglets, preventing and controlling animal diarrhea and promoting the production performance of the piglets.
(2) At present, the plant extract feed additive is mostly added in a mode of adding crushed materials into feed, and the processing technology comprises coarse powder, fine powder, superfine powder, low-temperature condensation superfine powder and the like. The addition technology is laggard behind, the dosage is large, the palatability is poor, the loss of active ingredients is large, the ingredients are complex, the action effect is unstable, the effective ingredients in the plant extract are not uniform, the balanced pharmacological action is difficult to play, the blindness is large, the product quality standard is difficult to formulate, the large-scale production, the popularization and the application are not facilitated, and the resource waste is serious. According to the method, the optimal production process is optimized by researching the active ingredients of the plant extracts, researching the aggregation parts of the plant active ingredients, adopting the processes of distillation extraction and the like and using single or combined process treatment, so that the content of the active ingredients is concentrated, the active substances are more stable, and substances for improving palatability are added to enhance the feed intake of piglets. The in-vitro antibacterial effect of the active ingredients of the plant extract is researched, the antibacterial spectrum and the antibacterial effect of the acidifier product are synthesized according to the antibacterial spectrum and the antibacterial ability, and the proportion of the plant extract is optimized.
The acidifier is an antibacterial rehabilitation feed additive accepted in the industry at present, short-chain fatty acids such as formic acid and acetic acid have good acidification effect, and meanwhile, the acidifier has good inhibition and killing effects on escherichia coli, salmonella and clostridium perfringens, and citric acid, fumaric acid, butyric acid and the like have good promotion effect on beneficial bacteria at the tail end of intestinal tracts. Many components of the acidifier can be directly absorbed and utilized by animals as nutritional ingredients of the animals. The acidifier has a very clear bacteriostatic mechanism, is not easy to generate tolerance for bacteria, and can be stably used for a long time.
The sodium diacetate has better acidification and corrosion prevention effects than sodium sorbate and has price advantage, so the sodium diacetate is selected as the first choice of the acidulant. Chlorogenic acid is a popular substitute for antibiotics in recent years, but chlorogenic acid from lonicera confusa and eucommia ulmoides is high in price, and chlorogenic acid content of the green coffee is high and low in price, so that the chlorogenic acid can be used as a substitute of an eucommia ulmoides extract. The macleaya cordata is also high in price, the price of 5% sanguinarine content reaches 650 yuan/kg, the macleaya cordata with the concentration of 1% is selected, the price is 70 yuan/kg, and the addition amount reaches 10%, so that the sanguinarine concentration can reach the level of 0.1%. The content of the contained active sanguinarine in the raw materials for treating the melania is about 0.137%, and the addition amount of the active sanguinarine is about 400g, so that the effect of replacing antibiotics can be achieved.
The main components of plant extracts are various, such as flavonoids, alkaloids, glycosides, quinones, phenylpropanoids, tannins, lipids, volatile oils, etc. The flavonoid products comprise ginkgetin, liquiritigenin and the like, while alkaloids such as sanguinarine extracted from macleaya cordata and the Meiyouzhu which takes the sanguinarine as a main active ingredient are the new stars of anti-diarrhea growth-promoting products in the industry at present. The volatile oil substances including cinnamaldehyde, carvacrol, eugenol and the like are used as main raw materials of essential oil products, have good antibacterial effect and fragrant smell, and can remarkably promote the increase of the feed intake of animals.
The potassium sorbate is a common intestinal acidifier, has the effects of sterilization and bacteriostasis, and can be used as one of the raw materials with lower cost. However, the amount should not be too high, since it tends to form lumps.
The small intestine is the main part of glutathione metabolism, Gln can synthesize purine nucleotide, pyrimidine nucleotide and important antioxidant glutathione, and is also the precondition of proline and arginine synthesis and the important component of body protein. Glutamine not only provides energy for the metabolism of intestinal mucosa epithelial cells, but also provides substrates for gluconeogenesis and urea synthesis of the liver. Research on intestinal nutrition of human and monogastric animals shows that Gln is an important energy source substance of intestinal mucosal cells and can promote growth, repair and integrity maintenance of mucosa. The use of a small amount of glutamine can help the recovery of intestinal mucosa of weaned pigs.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental reagents and materials involved are conventional biochemical reagents and materials unless otherwise specified.
Example 1
1. An intestinal health regulator for pigs comprises the following components in parts by weight: 60 parts of acidifier, 50 parts of plant extract, 3 parts of probiotics, 3 parts of saccharomyces cerevisiae extract, 2 parts of composite Chinese herbal medicine, 1 part of glutamine and 20 parts of coating composite vitamin.
Wherein the acidifying agent is a mixture of 10 parts of formic acid, 10 parts of citric acid, 10 parts of fumaric acid and 30 parts of a silica carrier.
The plant extract is a mixture of 10 parts of macleaya cordata extract sanguinarine, 10 parts of green coffee extract chlorogenic acid, 10 parts of liquiritigenin, 10 parts of cinnamaldehyde and 10 parts of eugenol.
The probiotics are a mixture of 5 hundred million U/g clostridium butyricum and 100 hundred million U/g lactobacillus.
The compound Chinese herbal medicine is a mixture of 1 part of campanumoea root and 1 part of hawthorn.
The core material coated with the compound vitamin comprises vitamin A and vitamin D3Vitamin E, vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Niacin, pantothenic acid, folic acid and biotin, wherein the wall material coated with the multivitamins comprises vegetable wax.
2. The preparation method of the intestinal health regulator for the pigs comprises the following steps: mixing 70% of the coated multivitamin, acidifying agent, probiotics, plant extract, saccharomyces cerevisiae extract, composite Chinese herbal medicine and glutamine, uniformly mixing, adding the rest coated multivitamin, and fully mixing to obtain the composite feed additive.
Example 2
1. An intestinal health regulator for pigs comprises the following components in parts by weight: 80 parts of acidifier, 40 parts of plant extract, 2 parts of probiotics, 5 parts of saccharomyces cerevisiae extract, 1 part of composite Chinese herbal medicine, 0.5 part of glutamine and 15 parts of coating composite vitamin.
Wherein the acidifying agent is a mixture of 10 parts of acetic acid, 10 parts of butyric acid, 20 parts of citric acid, 5 parts of sodium diacetate, 5 parts of potassium sorbate and 30 parts of silica carrier.
The plant extract is a mixture of 10 parts of macleaya cordata extract sanguinarine, 10 parts of ginkgetin and 20 parts of carvacrol.
The probiotics is a mixture of clostridium butyricum, lactobacillus and bacillus subtilis.
The compound Chinese herbal medicine is prepared by mixing 0.3 part of campanumoea root, 0.3 part of hawthorn and 0.4 part of dried orange peel.
The core material coated with the compound vitamin comprises vitamin A and vitamin D3Vitamin E, vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Nicotinic acid, pantothenic acid, folic acid and biotin, wherein the wall material for coating the multivitamins comprises vegetable waxOne or more of animal wax and gelatin.
2. The preparation method of the intestinal health regulator for the pigs comprises the following steps: mixing 80% of the coated multivitamin, acidifying agent, probiotics, plant extract, Saccharomyces cerevisiae extract, compound Chinese herbal medicine and glutamine, mixing uniformly, adding the rest coated multivitamin, and mixing fully to obtain the compound feed additive.
Example 3
1. An intestinal health regulator for pigs comprises the following components in parts by weight: 50 parts of acidifier, 20 parts of plant extract, 1 part of probiotics, 1 part of saccharomyces cerevisiae extract, 3 parts of composite Chinese herbal medicine, 1.5 parts of glutamine and 15 parts of coating composite vitamin.
Wherein the acidifying agent is a mixture of 10 parts of butyric acid, 10 parts of sodium diacetate, 10 parts of potassium sorbate and 20 parts of silica carrier.
The plant extract is mixture of sanguinarine extracted from Macleaya cordata, chlorogenic acid extracted from green coffee, liquiritigenin, ginkgetin, cinnamaldehyde and carvacrol.
The probiotics is a mixture of clostridium butyricum, lactobacillus and bacillus subtilis.
The compound Chinese herbal medicine is a mixture of 1 part of campanumoea root, 0.5 part of hawthorn, 1 part of Chinese date and 0.5 part of dried orange peel.
The core material coated with the compound vitamin comprises vitamin A and vitamin D3Vitamin E, vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12The wall material coated with the compound vitamin comprises one or more of vegetable wax, animal wax and gelatin.
2. The preparation method of the intestinal health regulator for the pigs comprises the following steps: mixing 75% of the coated multivitamin, acidifying agent, probiotics, plant extract, Saccharomyces cerevisiae extract, compound Chinese herbal medicine and glutamine, mixing uniformly, adding the rest coated multivitamin, and mixing fully to obtain the compound feed additive.
Study of the effects
1 materials and methods
1.1 test time and location
Test time: acclimatization is carried out for 1 week in 24 months in 2017 and 11 months in 2017, and formal test is carried out for 6 weeks; test site: jiangpu test pig farm of Nanjing university of agriculture.
1.2 test animals
90 healthy ordinary grade 'Du X big X long' ternary hybrid weaned piglets are purchased from Jiangpu experimental pig farm of Nanjing agriculture university. After adapting to the environment, the samples were randomly divided into 5 groups of 18 samples. The basal diet (blank control group), basal diet +1g/kg intestinal health regulator (0.1% group), basal diet +3g/kg intestinal health regulator (0.3% group), basal diet +5g/kg intestinal health regulator (0.5% group) and basal diet +3g/kg bacillus microecologics (microecologics group) were fed, respectively. The test period was 42 days.
1.3 test daily ration and additives
The test ration was a basal ration, as shown in table 1, to which 0.1% intestinal health regulator, 0.3% intestinal health regulator, 0.5% intestinal health regulator, and 0.3% bacillus microecological preparation were added, respectively. The intestinal health regulator is provided by Ohmei Biotechnology Inc. of Jiangsu, and the microecological preparation mainly comprises Bacillus and is commercially available.
TABLE 1 basal ration proportion and nutritional level for experimental piglets
1.4 growth Performance test
At 1d and last 1d morning 8: 00 empty stomach, and calculating average daily gain according to initial weight and final weight. Average daily gain-average net head gain/day of experiment. And recording the feed intake of the piglets, calculating the daily average feed intake, and calculating the feed-weight ratio after the experiment is finished. The feces of the piglets were observed regularly every day, the number of diarrhea heads was recorded, and the diarrhea rate (%) was calculated as the number of total diarrhea/(total number of heads × number of test days) × 100. The diarrhea index is the sum of stool scores/total number of test pigs (see table 2 for scoring criteria), and a higher index indicates more severe diarrhea.
TABLE 2 diarrhea score criteria
Table1 The standard scores of diarrhea in weaned piglets
1.5 mechanism test for anti-diarrhea
5mL of blood is collected from the early morning fasting vena cava of the piglet in the last 1d of the experiment, and the piglet is centrifuged at 3000 r/min for 10min to obtain serum. The contents of midgut trefoil factor (TFF3), Nitric Oxide (NO) and superoxide dismutase (SOD) in serum are detected by adopting an ELISA method according to the requirements of a kit specification.
Pig TFF3, NO and SOD detection kit, purchased from Assay Biotechnology Co., Ltd, USA. The main instruments include an enzyme-labeled detector (ELx800, BioTek), a centrifuge (5810R, Eppendorf), and the like.
1.8 statistical analysis of data
The results of all detection indexes are expressed in a form of Mean plus or minus standard deviation (Mean plus or minus SD), and ANOVA (one way analysis of variance) method in statistical software SPSS 17.0 is applied to carry out variance analysis, so that the significance of difference between each dosage group and microecological group of the intestinal health regulator and a blank control group is compared.
2 results and analysis
2.1 Effect of intestinal health regulators on weaned pig Productivity
The effect of the intestinal health modulator on the productivity of weaned piglets is shown in table 3. Wherein the daily gains of the 0.5 percent group and the 0.3 percent group of the intestinal health regulator are respectively extremely obvious (P <0.01) and obvious (P <0.05) higher than that of the blank control group, and the daily food intake of the 0.5 percent group and the 0.3 percent group of the intestinal health regulator are both extremely obvious (P <0.01) higher than that of the blank control group. The diarrhea rate and the diarrhea index of the intestinal health regulator of 0.5 percent and 0.3 percent are both very obvious (P <0.01) and are lower than those of a blank control group.
TABLE 3 Effect of intestinal health Modulator on weaned piglet Productivity
Table2 The growth performance of weaned piglets in every group
Note: p <0.05, P <0.01 compared to the blank control group. Note:p <0.05,. P <0.01, vs.
2.2 changes in the Effect factors for diarrhea
The content of the diarrhea influencing factors in each group is shown in Table 4. The content of TFF3 in the 0.5% intestinal health regulator group is 19.03 +/-2.07 ng/mL, compared with a blank control group, the difference is very obvious (P is less than 0.01), and the content of TFF3 in the 0.3% intestinal health regulator group is obviously higher (P is less than 0.05) than that in the blank control group. The content of NO in the 0.5% group of the intestinal health regulator is 36.91 +/-3.61 mu mol/L, compared with the blank control group, the difference is very obvious (P is less than 0.01), and the content of NO in the 0.3% group of the intestinal health regulator is obviously lower (P is less than 0.05) than that in the blank control group. The content of SOD in the 0.5% intestinal health regulator group is 11.35 +/-1.44 ng/mL, and compared with a blank control group, the difference is obvious (P is less than 0.05).
TABLE 4 diarrhea-affecting factor content in the animals of each group
Table3 The contents of diarrhea factor in every group
3 conclusion
3.1 the intestinal health regulator can effectively increase the daily gain of piglets, and compared with a blank control group, the 0.5 percent group and the 0.3 percent group have obvious difference and present a dose-effect relationship. The food intake of the intestinal health regulator in the 0.3% group was increased by 124.99 g/day compared with the control group, and the food intake in the 0.5% group was increased by 152.38 g/day.
3.20.5% of the group had a 67.11% reduction in the diarrhea rate of the intestinal health regulator compared to the control group, whereas the diarrhea rate in the 0.3% group was 55.35% reduction compared to the control group. The diarrhea rate and the diarrhea index of the intestinal health regulator of 0.5 percent and 0.3 percent are both very obvious (P <0.01) and are lower than those of a blank control group.
3.3 intestinal health regulator can induce the superoxide dismutase of free radical to express in large quantity, and at the same time, it can up-regulate small intestine trefoil factor to repair the intestinal mucosa damaged by nitric oxide, so as to reduce edema reaction and diarrhea syndrome of organism.
Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present invention.

Claims (7)

1. An intestinal health regulator for pigs comprises the following components in parts by weight: 50-80 parts of acidifier, 20-50 parts of plant extract, 1-3 parts of probiotics, 1-5 parts of saccharomyces cerevisiae extract, 1-3 parts of composite Chinese herbal medicine, 0.5-1.5 parts of glutamine and 15-35 parts of coated composite vitamin.
2. The porcine intestinal health modulator according to claim 1, wherein: the acidifying agent is selected from one or more of formic acid, acetic acid, butyric acid, citric acid, fumaric acid, sodium diacetate and potassium sorbate, and is mixed with a silicon dioxide carrier.
3. The porcine intestinal health modulator according to claim 1, wherein: the plant extract is one or more of macleaya cordata extract sanguinarine, green coffee extract chlorogenic acid, liquiritigenin, ginkgetin, cinnamaldehyde, carvacrol and eugenol.
4. The porcine intestinal health modulator according to claim 1, wherein: the probiotics are at least two of clostridium butyricum, lactobacillus and bacillus subtilis.
5. The porcine intestinal health modulator according to claim 1, wherein: the compound Chinese herbal medicine is a mixture of campanumoea root, hawthorn, Chinese date and dried orange peel.
6. The porcine intestinal health modulator according to claim 1, wherein: the core material coated with the compound vitamin comprises vitamin A and vitamin D3Vitamin E, vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12The wall material coated with the compound vitamin comprises one or more of vegetable wax, animal wax and gelatin.
7. A method for preparing the pig intestinal health regulator of claim 1, wherein the method comprises the steps of: the method comprises the following steps: mixing 70-80% of the formula amount of coated multivitamin with the formula amount of acidifier, probiotics, plant extract, Saccharomyces cerevisiae extract, compound Chinese herbal medicine and glutamine, mixing well, adding the rest coated multivitamin, and mixing well to obtain the compound feed additive.
CN201810619106.8A 2018-06-15 2018-06-15 Pig intestinal health regulator and preparation method thereof Pending CN110604225A (en)

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