CN110585357A - Pharmaceutical composition for preventing African swine fever and injection and application thereof - Google Patents

Abstract

The invention discloses a pharmaceutical composition for preventing African swine fever, and an injection and an application thereof, wherein the pharmaceutical composition comprises the following components in parts by weight: 10-1000 parts of gypsum, 5-200 parts of rhizoma anemarrhenae, 5-200 parts of lophatherum gracile, 5-200 parts of radix rehmanniae recen, 5-200 parts of radix scrophulariae, 5-200 parts of coptis chinensis, 5-200 parts of scutellaria baicalensis and 5-200 parts of gardenia jasminoides ellis. Extracting the above materials with water or ethanol, mixing to obtain medicinal extract, dissolving in water for injection, adding benzyl alcohol, tween-80 and injection active carbon, and filtering to obtain injection. The pharmaceutical composition has a prevention effect on various viral infections such as African swine fever virus, has no resistance and drug residues, has a remarkable effect on preventing African swine fever, and has an effective rate of about 80%.

Description

Pharmaceutical composition for preventing African swine fever and injection and application thereof

Technical Field

The invention belongs to the technical field of livestock breeding, and particularly relates to a pharmaceutical composition for preventing African swine fever, and an injection and application thereof.

Background

African swine fever, originally originated from African region, double-stranded DNA enveloped virus, is an acute, virulent, highly contact and lethal infectious disease of pigs and wild pigs caused by African swine fever virus family, African swine fever virus genus, African Swine Fever Virus (ASFV), and the pigs and wild pigs are the only natural hosts of the virus. The disease is not seasonal, and the transmission ways are contact transmission and entomophily transmission. It was first confirmed in kenya in 1921, introduced into europe in 1957, introduced into america in 1971, introduced into russia in 2007, introduced into ukraine in 2012, and introduced into white russia in 2013. Poland, Litaoza, Lasevian and Esania were introduced in 2014, Motorva was introduced in 2016, and Czech and Romania were introduced in 2017. It was introduced into Liaoning of China in 8 months in 2018. It is now endemic mainly in some regions of africa, europe, latin america. The world animal health Organization (OIE) classifies the animal epidemic disease as an essential report, and China classifies the animal epidemic disease as a type of animal epidemic disease.

Chinese plants are abundant in resources, and among these green plants, about 3000 kinds of medicinal plants closely related to human health are included, and 2598 kinds are included in the 2015 edition pharmacopeia. The plant berberine, naphthoquinone pigment, pinocyanin, chlorogenic acid and iridoid glycoside are widely present in plant organism, and at present, various plant berberine, naphthoquinone pigment, pinocyanin, chlorogenic acid and iridoid glycoside are separated and extracted, and have various biological effects, including antibacterial, antiviral, immunoregulation, anti-inflammatory, anti-tumor, blood sugar reducing, blood fat reducing, anti-radiation, anti-aging and other functions. Not only inhibits or kills the non-pestivirus, but also improves the resistance of the pig to the non-pestivirus, thereby achieving the purpose of effectively preventing the non-pestivirus.

At present, no effective medicine for preventing the African swine fever exists, an effective medicine for preventing the African swine fever is urgently needed, and the major problem in the live pig breeding is solved.

Disclosure of Invention

The invention aims to provide a pharmaceutical composition for preventing African swine fever, and an extract, an injection and application thereof.

The invention is realized by the following technical scheme:

a pharmaceutical composition for preventing African swine fever comprises the following components in parts by weight: 10-1000 parts of gypsum, 5-200 parts of rhizoma anemarrhenae, 5-200 parts of lophatherum gracile, 5-200 parts of radix rehmanniae recen, 5-200 parts of radix scrophulariae, 5-200 parts of coptis chinensis, 5-200 parts of scutellaria baicalensis and 5-200 parts of gardenia jasminoides ellis.

Preferably, the pharmaceutical composition for preventing African swine fever comprises the following components in parts by weight: 20-800 parts of gypsum, 10-150 parts of rhizoma anemarrhenae, 10-150 parts of lophatherum gracile, 10-100 parts of radix rehmanniae recen, 10-100 parts of radix scrophulariae, 10-100 parts of coptis chinensis, 10-100 parts of scutellaria baicalensis and 10-100 parts of gardenia jasminoides ellis.

More preferably, the pharmaceutical composition for preventing African swine fever comprises the following components in parts by weight: 400 parts of gypsum, 100 parts of rhizoma anemarrhenae, 100 parts of lophatherum gracile, 60 parts of radix rehmanniae, 60 parts of radix scrophulariae, 50 parts of coptis chinensis, 50 parts of scutellaria baicalensis and 50 parts of cape jasmine.

In another aspect of the present invention, there is provided a pharmaceutical extract for preventing african swine fever, comprising the above pharmaceutical composition.

The preparation method of the drug extract for preventing African swine fever comprises the following steps:

1) weighing gypsum, rhizoma anemarrhenae, lophatherum gracile, radix rehmanniae recen, radix scrophulariae, coptis chinensis, scutellaria baicalensis and gardenia according to the weight parts, and extracting with water or alcohol to obtain extracts of the components respectively;

2) mixing the extracts of the above components to obtain medicinal extract for preventing African swine fever.

In another aspect of the invention, a pharmaceutical injection for preventing African swine fever is provided, which comprises the above pharmaceutical composition.

The preparation method of the drug injection for preventing African swine fever comprises the following steps:

1) adding water for injection into the medicinal extract, and dissolving completely;

2) adding 0.8-1.2 ml of benzyl alcohol and 0.8-1.2 ml of Tween-80 into each 150ml of the mixture, and adjusting the pH value to 6.8-7.2;

3) adding injection active carbon with the mass fraction of 0.4-0.6%, fully stirring and dissolving, filtering to obtain filtrate, cooling, filtering, filling into an ampoule, and performing high-pressure moist heat sterilization at 121 ℃ for 10-20 min to obtain the injection.

Each 1ml of the medicine injection contains 2-4 g of the medicine composition.

In another aspect of the invention, the pharmaceutical composition is used for preparing a pharmaceutical preparation for preventing African swine fever.

In another aspect of the invention, the pharmaceutical extract is used for preparing a pharmaceutical preparation for preventing African swine fever.

The invention introduces the property, function or components of each medicinal material:

gypsum: the product is gypsum of anhydrite family of sulfate minerals, mainly containing hydrous calcium sulfate (CaSO)₄·2H₂O), sweet and pungent taste, cold nature, clear heat and purge fire, promote the production of body fluid to quench thirst, cool and disperse, clear internal excess heat. It is used to treat excessive stomach fire, headache, toothache, eczema, cough and asthma due to lung heat.

Rhizoma anemarrhenae: the product is a perennial herb of Anemarrhena of Liliaceae, and has bitter and sweet taste, cold nature, and effects of clearing heat and purging fire, promoting fluid production and moistening dryness. Can be used for treating exogenous febrile disease, hyperpyrexia with polydipsia, lung heat with cough, hectic fever due to yin deficiency, and constipation due to intestinal dryness.

Herba lophatheri: the product is dried aerial parts of Lophatherum gracile belonging to Lophatherum of Gramineae. Sweet and light taste, cold nature, clearing heat and relieving restlessness, promoting the production of body fluid and promoting urination. Can be used for treating fever polydipsia, infantile convulsion, cough with regurgitation, epistaxis, short urination, aphtha, and tongue sore.

Radix rehmanniae: it is a fresh or dry root tuber of rehmannia glutinosa Libosch of Scrophulariaceae, sweet, cold and moist in nature, bitter in flavor and capable of clearing heat and purging fire, cooling blood and nourishing yin. It is the essential herb for fever due to yin deficiency, recklessly blood flow due to blood heat and constipation due to dry body fluid. The radix rehmanniae extract has blood coagulation promoting effect. For weak heart, it has obvious cardiotonic action, mainly acting on cardiac muscle.

Figwort root: the product is the dried root of figwort root, which is a plant of Scrophulariaceae, and has the actions of nourishing yin to reduce fire, cooling blood to remove toxicity, relieving sore throat and relaxing bowel, and is a good herb for treating yin deficiency and internal heat, sore throat, and constipation due to intestinal dryness.

Coptis chinensis: the product is dried root of Coptidis rhizoma of perennial herb of Coptidis rhizoma of Ranunculaceae. Bitter taste and cold nature, clearing heat and drying dampness, purging fire and removing toxicity. Can be used for treating damp-heat distention, fullness, emesis, acid regurgitation, dysentery, jaundice, hyperpyrexia, coma, excessive heart-fire, vexation, insomnia, hematemesis, epistaxis, conjunctival congestion, toothache, diabetes, carbuncle, furuncle, etc.

Scutellaria baicalensis: the product is dried root of Scutellaria baicalensis Georgi of Scutellaria of Labiatae. Bitter taste and cold nature, clearing heat and drying dampness, purging fire and removing toxicity, stopping bleeding and preventing miscarriage. Can be used for treating epidemic febrile disease, upper respiratory infection, cough due to lung heat, yellow gallbladder due to damp-heat, pneumonia, dysentery, hemoptysis, conjunctival congestion, threatened abortion, hypertension, carbuncle, furuncle, and sore.

Gardenia: the product is dry fruit of Gardenia jasminoides Ellis of Gardenia of Rubiaceae, and has bitter and cold properties, and has effects of clearing heat, purging pathogenic fire, and cooling blood. Can be used for treating fever with vexation, insomnia, jaundice, gonorrhea, diabetes, conjunctival congestion, pharyngalgia, hematemesis, epistaxis, dysentery with blood, hematuria, pyocutaneous disease due to toxic heat, sprain, and swelling and pain.

The invention has the beneficial effects that:

1) aiming at that African swine fever is originally found in tropical regions and belongs to high fever, the selected raw materials of the medicinal materials belong to antibacterial and antiviral medicaments, the main components of the medicaments comprise berberine, naphthoquinone pigment, pinocyanin, chlorogenic acid and iridoid glycoside, the non-pestivirus entering the organism can be directly inhibited or killed, the functions of macrophages and T cells are enhanced, the number of E-ring formed cells is increased, cytokines are induced, interleukin (IL-2) is promoted to be generated, endogenous IFN is generated in the organism, and the two act together, so that the aim of effectively controlling the non-pestivirus invading the animal body is fulfilled.

2) The pharmaceutical composition has prevention effect on various viral infections such as African swine fever virus and the like, has no resistance and no drug residue, and simultaneously enhances the oxidation resistance level of organisms and inhibits the fat oxidation of muscles, thereby improving the quality of ketone bodies.

3) The components of the pharmaceutical composition provided by the invention act coordinately, inhibit or kill African swine fever virus together, and have a remarkable effect on preventing African swine fever, and the effective rate is about 80%.

4) The pharmaceutical composition can effectively prevent various viral diseases such as African swine fever, is low in cost and widely applicable, and can solve the major problems of the pork industry.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The test methods used in the examples of the present invention are all conventional methods unless otherwise specified.

The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.

Example 1

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 400 parts of gypsum, 100 parts of rhizoma anemarrhenae, 100 parts of lophatherum gracile, 60 parts of radix rehmanniae, 60 parts of radix scrophulariae, 50 parts of coptis chinensis, 50 parts of scutellaria baicalensis and 50 parts of cape jasmine. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing crude drug 3.0g per 1 ml.

Example 2

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 10 parts of gypsum, 5 parts of rhizoma anemarrhenae, 5 parts of lophatherum gracile, 5 parts of radix rehmanniae recen, 5 parts of radix scrophulariae, 5 parts of coptis chinensis, 5 parts of scutellaria baicalensis and 5 parts of cape jasmine. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing crude drug 2g per 1 ml.

Example 3

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 1000 parts of gypsum, 200 parts of rhizoma anemarrhenae, 200 parts of lophatherum gracile, 200 parts of radix rehmanniae recen, 200 parts of radix scrophulariae, 200 parts of coptis chinensis, 200 parts of scutellaria baicalensis and 200 parts of gardenia jasminoides. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing crude drug 4g per 1 ml.

Example 4

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 20 parts of gypsum, 10 parts of rhizoma anemarrhenae, 10 parts of lophatherum gracile, 10 parts of radix rehmanniae recen, 10 parts of radix scrophulariae, 10 parts of coptis chinensis, 10 parts of scutellaria baicalensis and 10 parts of cape jasmine. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing 2.5g crude drug per 1ml injection.

Example 5

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 800 parts of gypsum, 150 parts of rhizoma anemarrhenae, 150 parts of lophatherum gracile, 100 parts of radix rehmanniae recen, 100 parts of radix scrophulariae, 100 parts of coptis chinensis, 100 parts of scutellaria baicalensis and 100 parts of gardenia. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing crude drug 3.5g per 1 ml.

Example 6

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 1000 parts of gypsum, 100 parts of rhizoma anemarrhenae, 150 parts of lophatherum gracile, 150 parts of radix rehmanniae recen, 150 parts of radix scrophulariae, 150 parts of coptis chinensis, 150 parts of scutellaria baicalensis and 150 parts of cape jasmine. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing crude drug 3.8g per 1 ml.

Example 7

A pharmaceutical composition for preventing African swine fever comprises the following raw materials in parts by weight: 50 parts of gypsum, 70 parts of rhizoma anemarrhenae, 70 parts of lophatherum gracile, 55 parts of radix rehmanniae recen, 55 parts of radix scrophulariae, 55 parts of coptis chinensis, 55 parts of scutellaria baicalensis and 55 parts of gardenia jasminoides. Extracting the above materials with water or ethanol to obtain extract, and making into injection containing 2.9g crude drug per 1ml injection.

EXAMPLE 8 clinical trial

1000 pigs are fattened with good impurities in a farm, the pigs are 80-100 jin in weight, the pigs suddenly burst to generate high fever, 80% of the pigs have clinical symptoms of high fever, dyspnea, cyanosis of skin, depression, no food, no desire to drink water, constipation and bloody appearance, the pigs are treated by various antibiotics and antipyretics, and the pigs begin to die on day 2. The test paper detects that the African swine fever virus is confirmed to be infected.

Under the guidance of professional veterinarians, 350 infected pigs in the batch are randomly selected as an administration group, the group is divided into 7 groups, 50 pigs in each group are respectively and correspondingly used by the injection prepared in the embodiment 1-7 of the invention, and the experiment is carried out, wherein intramuscular injection (ear root back neck side) and drinking water are synchronously carried out. Injecting into muscle 10 ml/time (the dosage is 20 ml/time above 100kg body weight), continuously using for 3 days, 2 times per day, and 10-12 hr apart each time; 200ml of drinking water is mixed with 50kg of water, and the drinking water is intensively drunk in the afternoon for 10 days continuously. The batch was randomly selected to infect 50 pigs and fed normally as a control group. The post-dose prophylaxis results are reported in table 1.

TABLE 1 post-dose prophylaxis results recording

The statistical results are shown in table 2.

TABLE 2 statistical results

	Total number (head)	Cure (head)	Death (head)	Cure rate (%)	Mortality (%)
						Example 1	50	39	11	78.0	22.0
Example 2	50	39	11	78.0	22.0
						Example 3	50	38	12	76.0	24.0
Example 4	50	39	11	78.0	22.0
						Example 5	50	40	10	80.0	20.0
Example 6	50	40	10	80.0	20.0
						Example 7	50	40	10	80.0	20.0
Control group	50	0	50	0.0	100.0

The clinical tests show that the pharmaceutical composition has a remarkable effect on preventing African swine fever, and the cure rate is about 80%. The pharmaceutical composition can effectively control the disease deterioration, recover drinking water, recover food intake and recover normal growth. Therefore, the pharmaceutical composition can be applied to the preparation of the drugs for preventing African swine fever viruses.

In addition, under the supervision of the relevant competent departments, 100 healthy pigs were randomly selected, and the injection prepared in example 1 of the present invention was used for the experiment, and intramuscular injection (on the back of the ear root and the neck) of pigs was performed in synchronization with drinking water. Intramuscular injection for 10 ml/time (the dosage is 20 ml/time above 100kg body weight), only for 1 day, and for 2 times in 1 day; 200ml of drinking water is mixed with 50kg of water, and the drinking water is intensively drunk in the afternoon for 3 days continuously. After that, the healthy pigs were placed in an African swine fever virus environment, 3 pigs died 30 days later, and 97 pigs kept healthy growth. Clinical tests show that when the injection (intramuscular injection and drinking with water) prepared in the embodiment 1 of the invention is used by healthy pigs, the African swine fever virus can be prevented from being infected, and the pharmaceutical composition can also be applied to preparation of drugs for preventing the African swine fever virus from being infected.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The pharmaceutical composition for preventing African swine fever is characterized by comprising the following components in parts by weight: 10-1000 parts of gypsum, 5-200 parts of rhizoma anemarrhenae, 5-200 parts of lophatherum gracile, 5-200 parts of radix rehmanniae recen, 5-200 parts of radix scrophulariae, 5-200 parts of coptis chinensis, 5-200 parts of scutellaria baicalensis and 5-200 parts of gardenia jasminoides ellis.

2. The pharmaceutical composition for preventing African swine fever according to claim 1, which comprises the following components in parts by weight: 20-800 parts of gypsum, 10-150 parts of rhizoma anemarrhenae, 10-150 parts of lophatherum gracile, 10-100 parts of radix rehmanniae recen, 10-100 parts of radix scrophulariae, 10-100 parts of coptis chinensis, 10-100 parts of scutellaria baicalensis and 10-100 parts of gardenia jasminoides ellis.

3. The pharmaceutical composition for preventing African swine fever according to claim 2, which comprises the following components in parts by weight: 400 parts of gypsum, 100 parts of rhizoma anemarrhenae, 100 parts of lophatherum gracile, 60 parts of radix rehmanniae, 60 parts of radix scrophulariae, 50 parts of coptis chinensis, 50 parts of scutellaria baicalensis and 50 parts of cape jasmine.

4. A pharmaceutical extract for preventing african swine fever, comprising the pharmaceutical composition of claim 1.

5. The drug extract for preventing African swine fever according to claim 4, wherein the preparation method of the drug extract comprises the following steps:

1) weighing gypsum, rhizoma anemarrhenae, lophatherum gracile, radix rehmanniae recen, radix scrophulariae, coptis chinensis, scutellaria baicalensis and gardenia according to the weight parts, and extracting with water or alcohol to obtain extracts of the components respectively;

2) mixing the extracts of the above components to obtain medicinal extract for preventing African swine fever.

6. A pharmaceutical injection for preventing african swine fever, which comprises the pharmaceutical composition of claim 1.

7. The pharmaceutical injection for preventing African swine fever according to claim 6, wherein the preparation method comprises the following steps:

1) adding water for injection into the medicinal extract, and dissolving completely;

2) adding 0.8-1.2 ml of benzyl alcohol and 0.8-1.2 ml of Tween-80 into each 150ml of the mixture, and adjusting the pH value to 6.8-7.2;

3) adding injection active carbon with the mass fraction of 0.4-0.6%, fully stirring and dissolving, filtering to obtain filtrate, cooling, filtering, filling into an ampoule, and performing high-pressure moist heat sterilization at 121 ℃ for 10-20 min to obtain the injection.

8. Use of the pharmaceutical composition of any one of claims 1-3 for the preparation of a pharmaceutical preparation for preventing African swine fever.

9. Use of the pharmaceutical composition according to any one of claims 4 to 5 for the preparation of a pharmaceutical preparation for preventing African swine fever.