CN110583881A - Fermented traditional Chinese medicine additive for improving sow infertility and preparation method thereof - Google Patents

Fermented traditional Chinese medicine additive for improving sow infertility and preparation method thereof Download PDF

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CN110583881A
CN110583881A CN201910840574.2A CN201910840574A CN110583881A CN 110583881 A CN110583881 A CN 110583881A CN 201910840574 A CN201910840574 A CN 201910840574A CN 110583881 A CN110583881 A CN 110583881A
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李靠兴
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Yangling Tai Lai De Biotechnology Co Ltd
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Yangling Tai Lai De Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines

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Abstract

The invention relates to a fermented traditional Chinese medicine additive for improving sow infertility and a preparation method thereof, the fermented traditional Chinese medicine additive is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid, and the traditional Chinese medicine components comprise the following components in parts by weight: 20-40% of Chinese date, 4-6% of astragalus, 5-15% of eucommia bark, 5-15% of epimedium, 2-4% of medlar, 2-4% of motherwort, 5-15% of dogwood, 5-15% of rhizoma alismatis and 10-30% of oyster; the bacterial liquid comprises 0.03-0.07% of yeast liquid, 0.05-0.15% of lactobacillus acidophilus liquid and 0.02-0.08% of bacillus subtilis liquid; the preparation method of the fermented traditional Chinese medicine additive mainly comprises the following steps: pulverizing the Chinese medicinal materials, sieving, adding manganese sulfate, zinc sulfate, 1% sodium selenite and 50% VE, mixing, adding bacterial solution, fermenting under sealed condition to obtain fermented product, oven drying, and pulverizing. The fermented traditional Chinese medicine additive can treat the infertility of the sow, promote the rapid development and maturation of the ovary of the sow, stimulate the ovulation of the ovary, promote the formation of progesterone and enhance the reproductive productivity of the sow.

Description

Fermented traditional Chinese medicine additive for improving sow infertility and preparation method thereof
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a fermented traditional Chinese medicine additive for improving the infertility of sows and a preparation method thereof.
Background
In the large-scale breeding process, the livestock and poultry infertility not only affects the farrowing amount of the sows, but also directly affects the pig raising cost. Infertility of female livestock is caused by malnutrition, hormone endocrine disturbance, environmental stress, certain diseases and the like. The sterility of male animals is caused by congenital deficiency, over-breeding, kidney injury due to long-term illness or senile asthenia. Most of the livestock and poultry are manifested by lassitude, low vitality and easy fatigue; intolerance of cold; hyposexual function, retronaral shift in primiparity and postpartum anestrus. The livestock and poultry infertility directly affects the economic benefit of production and causes serious economic loss.
Aiming at the problem, in the prior art, the estrus can be induced by chemical injections such as intramuscular injection of pregnant mare serum, chlorprostenol and the like, but the estrus return rate and the nonpregnant rate are higher than 50%, the farrowing rate is low, and the phenomenon of difficult delivery caused by overlarge fetus during delivery is easy to occur, so that the dead fetus and the sow rejection rate are increased.
According to the guidance of the theory of traditional Chinese medicine, aiming at the clinical symptoms of sow infertility, postpartum anestrus, early-phase retrogradation and the like, starting from the aspects of warming and invigorating kidney yang, nourishing liver and kidney, tonifying kidney and promoting estrus, promoting blood circulation and regulating menstruation and the like, a traditional Chinese medicine compound consisting of Chinese date, astragalus, eucommia, epimedium, medlar, motherwort, dogwood, rhizoma alismatis and oyster is developed, and the fermented traditional Chinese medicine additive for treating the sow infertility is developed and developed through closed fermentation of saccharomycete liquid, lactobacillus acidophilus liquid and bacillus subtilis liquid.
Disclosure of Invention
In order to solve the problems that the effect of the sow infertility is poor and negative effects are generated by injecting chemical injection, the invention provides the fermented traditional Chinese medicine additive for improving the sow infertility and the preparation method thereof.
The technical problem to be solved by the invention is realized by the following technical scheme:
a fermented Chinese medicinal additive for improving sow infertility is prepared by fermenting Chinese medicinal components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 20-40% of Chinese date, 4-6% of astragalus, 5-15% of eucommia bark, 5-15% of epimedium, 2-4% of medlar, 2-4% of motherwort, 5-15% of dogwood, 5-15% of rhizoma alismatis and 10-30% of oyster; the bacterial liquid comprises 0.03-0.07% of yeast liquid, 0.05-0.15% of lactobacillus acidophilus liquid and 0.02-0.08% of bacillus subtilis liquid.
Preferably, the fermented traditional Chinese medicine additive is prepared by compounding and fermenting traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 25-35% of Chinese date, 5-6% of astragalus, 8-12% of eucommia bark, 8-12% of epimedium, 2-3% of medlar, 2-3% of motherwort, 8-12% of dogwood, 8-12% of rhizoma alismatis and 15-25% of oyster; the bacterial liquid comprises 0.04-0.06% of yeast liquid, 0.08-0.12% of lactobacillus acidophilus liquid and 0.04-0.06% of bacillus subtilis liquid.
Preferably, the fermented traditional Chinese medicine additive is prepared by compounding and fermenting traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 30% of Chinese date, 5% of astragalus, 10% of eucommia, 10% of epimedium, 2.5% of medlar, 2.5% of motherwort, 10% of dogwood, 10% of rhizoma alismatis and 19.8% of oyster; the bacterial liquid comprises 0.05% of saccharomycete liquid, 0.1% of acidophilic lactobacillus liquid and 0.05% of bacillus subtilis liquid.
Preferably, the viable bacteria content of the yeast bacterial liquid is 1.8-2.2 × 1010CFU/g, the viable bacteria content of the acidophilic lactobacillus bacterial liquid is 8-12 multiplied by 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 8-12 multiplied by 1010CFU/g。
Preferably, the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, the viable bacteria content of the acidophilic lactobacillus bacterial liquid is 10 multiplied by 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
The preparation method of the fermented traditional Chinese medicine additive comprises the following steps:
the method comprises the following steps: treating Chinese dates: cleaning the Chinese dates, and then drying;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 60-120 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% of sodium selenite and 50% of VE into the mixture A, and mixing to obtain a mixture B;
step four: and (3) probiotic compound fermentation: adding saccharomycete liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid into the mixture B, and sealing and fermenting to obtain a fermentation product C;
step five: and (3) low-temperature drying: drying the fermentation product C at low temperature;
step six: crushing: and (3) crushing the dried fermentation product C, and sieving by a 60-120-mesh sieve to obtain the traditional Chinese medicine fermentation additive.
Preferably, the drying temperature in the first step is 75-85 ℃, and the drying time is 20-30 h.
Preferably, in the third step, the content of manganese sulfate is 0.15-0.25%, the content of zinc sulfate is 0.15-0.25%, the content of 1% sodium selenite is 0.01-0.02%, and the content of 50% VE is 0.05-0.15%.
Preferably, the fermentation temperature in the fourth step is 30-45 ℃, and the fermentation time is 72-108 h.
Preferably, the drying temperature in the fifth step is 15-36 ℃, and the drying time is 40-60 h.
Compared with the prior art, the invention has the beneficial effects that:
1. the fermented traditional Chinese medicine additive is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid, wherein the traditional Chinese medicine components comprise Chinese date, astragalus membranaceus, eucommia ulmoides, epimedium, Chinese wolfberry, motherwort, dogwood, rhizoma alismatis and oyster, can treat infertility of sows, can promote rapid development and maturity of ovaries of sows, stimulate ovarian ovulation, promote formation of progesterone, enhance and recover reproductive productivity of the sows, are natural in origin, and have no side effect;
2. according to the invention, Chinese date, astragalus, eucommia bark, epimedium herb, medlar, motherwort herb, dogwood, alisma orientale and oyster are subjected to closed fermentation by yeast liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid, so that the active ingredients of the traditional Chinese medicines are released to the maximum extent, the efficacy of the traditional Chinese medicines is obviously improved, and the effect of improving the infertility of sows is achieved.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the embodiments of the present invention are not limited thereto.
Example 1:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 40% of Chinese date, 4% of astragalus, 5% of eucommia, 5% of epimedium, 4% of medlar, 4% of motherwort, 5% of dogwood, 5% of rhizoma alismatis and 27.8% of oyster; the bacterial liquid comprises 0.03 percent of saccharomycete liquid, 0.15 percent of acidophilic lactobacillus liquid and 0.02 percent of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 1.8 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 10 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
Example 2:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 20% of Chinese date, 6% of astragalus, 14.8% of eucommia bark, 15% of epimedium, 2% of medlar, 2% of motherwort, 15% of dogwood, 15% of rhizoma alismatis and 10% of oyster; the bacterial liquid comprises 0.07% of yeast liquid, 0.05% of lactobacillus acidophilus liquid and 0.08% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 8 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 12 multiplied by 1010CFU/g。
Example 3:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 25% of Chinese date, 5% of astragalus, 15% of eucommia bark, 5.8% of epimedium, 3% of medlar, 2.8% of motherwort, 12% of dogwood, 12% of rhizoma alismatis and 15% of oyster; the bacterial liquid comprises 0.05% of saccharomycete liquid, 0.1% of acidophilic lactobacillus liquid and 0.05% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2.2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 12 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 8 multiplied by 1010CFU/g。
Example 4:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 35% of Chinese date, 4% of astragalus, 8% of eucommia, 8% of epimedium, 3% of medlar, 2.8% of motherwort, 12% of dogwood, 12% of rhizoma alismatis and 15% of oyster; the bacterial liquid comprises 0.06% of yeast liquid, 0.1% of lactobacillus acidophilus liquid and 0.04% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 9 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
Example 5:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 24% of Chinese date, 5% of astragalus, 12% of eucommia, 12% of epimedium, 2.8% of medlar, 3% of motherwort, 8% of dogwood, 8% of rhizoma alismatis and 25% of oyster; the bacterial liquid comprises 0.05% of yeast liquid, 0.08% of lactobacillus acidophilus liquid and 0.07% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 10 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 9 multiplied by 1010CFU/g。
Example 6:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 29% of Chinese date, 5% of astragalus, 10% of eucommia, 10% of epimedium, 2.5% of medlar, 3.3% of motherwort, 10% of dogwood, 10% of rhizoma alismatis and 20% of oyster; the bacterial liquid comprises 0.04% of yeast liquid, 0.1% of lactobacillus acidophilus liquid and 0.06% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 10 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
Example 7:
the fermented traditional Chinese medicine additive for improving the infertility of the sows is prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 30% of Chinese date, 5% of astragalus, 10% of eucommia, 10% of epimedium, 2.5% of medlar, 2.5% of motherwort, 10% of dogwood, 10% of rhizoma alismatis and 19.8% of oyster; the bacterial liquid comprises 0.05% of saccharomycete liquid, 0.1% of acidophilic lactobacillus liquid and 0.05% of bacillus subtilis liquid.
Wherein the viable bacteria content of the yeast bacterial liquid is 2 × 1010CFU/g, viable bacteria content of Lactobacillus acidophilus liquid is 10 × 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
Example 8:
the fermented traditional Chinese medicine additive for improving the infertility of sows comprises the following components in parts by weight: 30% of Chinese date, 5% of astragalus, 10% of eucommia, 10% of epimedium, 2.5% of medlar, 2.5% of motherwort, 10% of dogwood, 10% of rhizoma alismatis and 20% of oyster.
Example 8 is a comparative example of example 7, in which the components and contents of the Chinese herbal medicines are the same, except that example 8 does not add yeast liquid, lactobacillus acidophilus liquid and bacillus subtilis liquid for fermentation.
Example 1-3 a method for preparing a fermented herbal supplement, comprising the steps of:
the method comprises the following steps: treating Chinese dates: cleaning Chinese dates, and then drying the Chinese dates at 75 ℃ for 30 hours;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 60 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% sodium selenite and 50% VE into the mixture A, and mixing to obtain a mixture B, wherein the content of manganese sulfate is 0.15%, the content of zinc sulfate is 0.25%, the content of 1% sodium selenite is 0.01%, and the content of 50% VE is 0.15%;
step four: and (3) probiotic compound fermentation: adding yeast liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid into the mixture B, and performing closed fermentation to obtain a fermentation product C, wherein the fermentation temperature is 30 ℃ and the fermentation time is 108 h;
step five: and (3) low-temperature drying: drying the fermentation product C at a low temperature of 36 ℃ for 40 h;
step six: crushing: and (4) crushing the dried fermentation product C, and sieving with a 60-mesh sieve to obtain the traditional Chinese medicine fermentation additive.
Example 4-6A method for preparing a fermented herbal supplement, comprising the steps of:
the method comprises the following steps: treating Chinese dates: cleaning the Chinese dates, and then drying the Chinese dates at the drying temperature of 85 ℃ for 20 hours;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 120 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% sodium selenite and 50% VE into the mixture A, and mixing to obtain a mixture B, wherein the content of manganese sulfate is 0.25%, the content of zinc sulfate is 0.15%, the content of 1% sodium selenite is 0.02%, and the content of 50% VE is 0.05%;
step four: and (3) probiotic compound fermentation: adding yeast liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid into the mixture B, and performing closed fermentation to obtain a fermentation product C, wherein the fermentation temperature is 45 ℃ and the fermentation time is 72 hours;
step five: and (3) low-temperature drying: drying the fermentation product C at a low temperature of 15 ℃ for 60 h;
step six: crushing: and (4) crushing the dried fermentation product C, and sieving with a 120-mesh sieve to obtain the traditional Chinese medicine fermentation additive.
Example 7 a method for preparing a fermented herbal supplement, comprising the steps of:
the method comprises the following steps: treating Chinese dates: cleaning the Chinese dates, and then drying the Chinese dates at the drying temperature of 85 ℃ for 26 hours;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 80 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% sodium selenite and 50% VE into the mixture A, and mixing to obtain a mixture B, wherein the content of manganese sulfate is 0.2%, the content of zinc sulfate is 0.2%, the content of 1% sodium selenite is 0.01%, and the content of 50% VE is 0.1%;
step four: and (3) probiotic compound fermentation: adding saccharomycete liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid into the mixture B, and sealing and fermenting to obtain a fermentation product C, wherein the fermentation temperature is 35 ℃ and the fermentation time is 96 hours;
step five: and (3) low-temperature drying: drying the fermentation product C at a low temperature of 35 ℃ for 48 h;
step six: crushing: and (4) crushing the dried fermentation product C, and sieving with a 80-mesh sieve to obtain the traditional Chinese medicine fermentation additive.
The preparation method of the traditional Chinese medicine additive comprises the following steps:
the method comprises the following steps: treating Chinese dates: cleaning the Chinese dates, and then drying the Chinese dates at the drying temperature of 85 ℃ for 26 hours;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 80 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% sodium selenite and 50% VE into the mixture A, and mixing to obtain a mixture B, wherein the content of manganese sulfate is 0.2%, the content of zinc sulfate is 0.2%, the content of 1% sodium selenite is 0.01%, and the content of 50% VE is 0.1%;
step four: and (3) low-temperature drying: drying the mixture B at a low temperature of 35 ℃ for 48 h;
step five: crushing: and (4) crushing the dried mixture B, and sieving the crushed mixture B with a 80-mesh sieve to obtain the traditional Chinese medicine additive.
In order to verify the performance of the fermented traditional Chinese medicine additive of the invention, the following tests were carried out:
experimental materials: (1) the fermented traditional Chinese medicine additive prepared in example 7 above and the traditional Chinese medicine additive prepared in example 8 above; (2) animals: 100 sows which do not have oestrus for a long time, have slight oestrus reaction and no mature ovum discharge due to slow ovarian development are selected, and the sows are 8-10 months old. The test site is in a certain breeding farm of the Yangtze.
Group of Number of cases/case Usage amount/times of use/day Method of administration
First group 20 300g/1 times/day Adding into feed for eating
Second group 20 200g/1 times/day Adding into feed for eating
Third group 20 100g/1 times/day Adding into feed for eating
Fourth group 20 300g/1 times/day Adding into feed for eating
Fifth group 20 0g/0 times/day /
The first, second and third groups all consumed the feed added with the fermented traditional Chinese medicine additive prepared in example 7, and the consumption of the first, second and third groups was decreased in order. The fourth group was the traditional Chinese medicine additive prepared in example 8, and the fifth group did not eat any additive.
After the medicine is taken for 12 hours, the boars with vigorous sexual desire, no diseases and wearing heat-testing cloth are used for heat testing every 12 hours, the sows approach the boars, the boars shake the tails and accept boar strides or boar strides are judged to be in heat, and the heat-producing persons are effective for heat treatment in the same period within 120 hours. And observing the ovulation on the ovary and the corpus luteum development of the estrus sow by using a laparoscope 5 days after the estrus.
And (3) curing: the sow is close to the boar, the tail is shaken, and the boar strides or the sows stride each other are judged to be oestrous, and the ovary ovulation and corpus luteum development are normal.
The method has the following advantages: sow close to boar, tail shake, ovulation on ovary and corpus luteum development.
And (4) invalidation: the sow is close to the boar, the tail is not shaken, and the boar climbing span is not accepted. Ovulation on the ovary and retarded or no development of the corpus luteum.
After 9-10 days, the sows in the fifth group approach the boars without shaking tails and do not accept the boar climbing. Ovulation on the ovary and retarded or no development of the corpus luteum. 20 sick pigs were unchanged.
After 9-10 days, 3 sows in the fourth group approach the boar and do not shake the tail, do not accept the boar to climb, ovulate in the ovary and delay or do not develop corpus luteum; the 12 sows are close to the boars, the tails are shaken, the ovaries are ovulated and the corpus luteum is developed; the 5 sows are close to the boars, and the boars who shake the tails and accept the boars or the sows climb each other are judged to be oestrous, ovulate in the ovaries and have normal corpus luteum development.
After 9-10 days, 7 sows approach the boar, the tail is shaken, the ovary ovulates and the corpus luteum develops; and 13 pigs approach the boar and shake the tail, and are subjected to boar climbing or sow mutual climbing. Ovulation on the ovary and corpus luteum development are normal.
After 9-10 days, 4 sows approach the boar, the tail is shaken, the ovary is ovulated and the corpus luteum is developed; the 16 pigs approach the boar and shake the tail, and are subjected to boar climbing or sow mutual climbing. Ovulation on the ovary and corpus luteum development are normal.
After 9-10 days of the first group of sows, 3 sows approach the boar, the tail is shaken, the ovary is ovulated and the corpus luteum is developed; and (5) shaking the tail of 17 pigs close to the boars, and receiving the boar climbing or the sow mutual climbing. Ovulation on the ovary and corpus luteum development are normal.
Conclusion the treatment test effect on the sick sows shows that the traditional Chinese medicine additive which is not added with the yeast liquid, the lactobacillus acidophilus liquid and the bacillus subtilis liquid for closed fermentation has a treatment effect on sows which do not estrus or have slight estrous reaction and have no mature ovum discharge due to slow ovarian development; the fermented traditional Chinese medicine additive has obvious treatment effect on sows which are not oestrous or have slight oestrus reaction and have no mature ovum discharge due to slow ovarian development, and has better effect than the traditional Chinese medicine additive which is not added with saccharomycete liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid and is subjected to closed fermentation. The sows fed with the fermented traditional Chinese medicine additive are continuously observed, no diseases appear in the sows after 1 month of use, and the growth indexes are normal. The fermented traditional Chinese medicine additive is safe and has no adverse effect.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.

Claims (10)

1. A fermented traditional Chinese medicine additive for improving sow infertility is characterized by being prepared by compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 20-40% of Chinese date, 4-6% of astragalus, 5-15% of eucommia bark, 5-15% of epimedium, 2-4% of medlar, 2-4% of motherwort, 5-15% of dogwood, 5-15% of rhizoma alismatis and 10-30% of oyster; the bacterial liquid comprises 0.03-0.07% of yeast liquid, 0.05-0.15% of lactobacillus acidophilus liquid and 0.02-0.08% of bacillus subtilis liquid.
2. The additive of fermented traditional Chinese medicine according to claim 1, which is prepared by the compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 25-35% of Chinese date, 5-6% of astragalus, 8-12% of eucommia bark, 8-12% of epimedium, 2-3% of medlar, 2-3% of motherwort, 8-12% of dogwood, 8-12% of rhizoma alismatis and 15-25% of oyster; the bacterial liquid comprises 0.04-0.06% of yeast liquid, 0.08-0.12% of lactobacillus acidophilus liquid and 0.04-0.06% of bacillus subtilis liquid.
3. The additive of fermented traditional Chinese medicine according to claim 1, which is prepared by the compound fermentation of traditional Chinese medicine components and bacterial liquid; the traditional Chinese medicine comprises the following components in parts by weight: 30% of Chinese date, 5% of astragalus, 10% of eucommia, 10% of epimedium, 2.5% of medlar, 2.5% of motherwort, 10% of dogwood, 10% of rhizoma alismatis and 19.8% of oyster; the bacterial liquid comprises 0.05% of saccharomycete liquid, 0.1% of acidophilic lactobacillus liquid and 0.05% of bacillus subtilis liquid.
4. The fermented traditional Chinese medicine additive according to claim 1, wherein the viable bacteria content of the yeast bacterial liquid is 1.8-2.2 x 1010CFU/g, the viable bacteria content of the acidophilic lactobacillus bacterial liquid is 8-12 multiplied by 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 8-12 multiplied by 1010CFU/g。
5. The fermented traditional Chinese medicine additive according to claim 1, wherein the viable bacteria content of the yeast bacterial liquid is 2 x 1010CFU/g, the viable bacteria content of the acidophilic lactobacillus bacterial liquid is 10 multiplied by 1010CFU/g, the viable bacteria content of the bacillus subtilis liquid is 10 multiplied by 1010CFU/g。
6. A method for preparing the fermented traditional Chinese medicine additive according to any one of claims 1 to 5, which comprises the following steps:
the method comprises the following steps: treating Chinese dates: cleaning the Chinese dates, and then drying;
step two: crushing medicinal materials: pulverizing fructus Jujubae, radix astragali, Eucommiae cortex, herba Epimedii, fructus Lycii, herba Leonuri, Corni fructus, Alismatis rhizoma and Concha Ostreae, sieving with 60-120 mesh sieve, and mixing to obtain mixture A;
step three: adding manganese sulfate, zinc sulfate, 1% of sodium selenite and 50% of VE into the mixture A, and mixing to obtain a mixture B;
step four: and (3) probiotic compound fermentation: adding saccharomycete liquid, acidophilic lactobacillus liquid and bacillus subtilis liquid into the mixture B, and sealing and fermenting to obtain a fermentation product C;
step five: and (3) low-temperature drying: drying the fermentation product C at low temperature;
step six: crushing: and (3) crushing the dried fermentation product C, and sieving by a 60-120-mesh sieve to obtain the traditional Chinese medicine fermentation additive.
7. The preparation method according to claim 6, wherein the drying temperature in the first step is 75-85 ℃ and the drying time is 20-30 h.
8. The preparation method of claim 6, wherein the content of manganese sulfate in the third step is 0.15-0.25%, the content of zinc sulfate is 0.15-0.25%, the content of 1% sodium selenite is 0.01-0.02%, and the content of 50% VE is 0.05-0.15%.
9. The preparation method of claim 6, wherein the temperature of the fermentation in the fourth step is 30-45 ℃ and the fermentation time is 72-108 h.
10. The preparation method according to claim 6, wherein the drying temperature in the fifth step is 15-36 ℃ and the drying time is 40-60 h.
CN201910840574.2A 2019-09-06 2019-09-06 Fermented traditional Chinese medicine additive for improving sow infertility and preparation method thereof Pending CN110583881A (en)

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CN103947905A (en) * 2014-05-19 2014-07-30 湖南农业大学 Heat-stress-resistant traditional Chinese medicine microbial feed additive specially used for sows, and preparation method of feed additive
CN109247444A (en) * 2018-11-26 2019-01-22 山西大禹生物工程股份有限公司 A kind of feed addictive and preparation method thereof conducive to sow Recovery

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