CN110547469A - Probiotic composition and preparation method thereof - Google Patents

Probiotic composition and preparation method thereof Download PDF

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Publication number
CN110547469A
CN110547469A CN201910885310.9A CN201910885310A CN110547469A CN 110547469 A CN110547469 A CN 110547469A CN 201910885310 A CN201910885310 A CN 201910885310A CN 110547469 A CN110547469 A CN 110547469A
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Prior art keywords
powder
parts
probiotic composition
vitamin
bifidobacterium
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CN201910885310.9A
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Chinese (zh)
Inventor
袁海华
黄园
王岚
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Shenzhen Catic Life Health Technology Co ltd
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Shenzhen Catic Life Health Technology Co ltd
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Priority to CN201910885310.9A priority Critical patent/CN110547469A/en
Publication of CN110547469A publication Critical patent/CN110547469A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/519—Breve
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/531—Lactis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a probiotic composition for regulating intestinal bacterial colony, which comprises the following components in parts by weight: 4 to 6 portions of bifidobacterium lactis powder, 3 to 5 portions of bifidobacterium longum powder, 4 to 6 portions of bifidobacterium breve powder freeze-dried powder, 30 to 34 portions of oligosaccharide, 3 to 5 portions of lactobacillus acidophilus powder, 15 to 20 portions of skimmed milk powder and 0.01 to 0.05 portion of vitamin. The invention also discloses a preparation method of the probiotic composition. The probiotic composition disclosed by the invention can prevent intestinal diseases of human bodies.

Description

Probiotic composition and preparation method thereof
Technical Field
The invention relates to the technical field of probiotics, in particular to a probiotic composition and a preparation method thereof.
Background
Probiotics refer to active microorganisms having an excellent influence on humans, animals, and the like. People can protect the intestinal tract of the human body by taking the probiotics, thereby maintaining the normal metabolism of the human body.
With the acceleration of the life rhythm of people, the life and the working pressure of people are increased, so that the eating rule is disturbed, the nutrient intake is disordered, and the intestinal diseases are caused.
the above is only for the purpose of assisting understanding of the technical aspects of the present invention, and does not represent an admission that the above is prior art.
disclosure of Invention
The main object of the present invention is to provide a probiotic composition and a method for preparing the same, wherein the probiotic composition comprises milk bifidobacterium powder, bifidobacterium longum powder, bifidobacterium breve powder lyophilized powder, lactobacillus acidophilus powder and other probiotics, and the content of the probiotics in the human intestinal tract is increased by taking the probiotic composition, so as to prevent intestinal diseases.
The invention provides a probiotic composition for regulating intestinal bacterial colony, which comprises the following components in parts by weight:
4 to 6 portions of bifidobacterium lactis powder, 3 to 5 portions of bifidobacterium longum powder, 4 to 6 portions of bifidobacterium breve powder freeze-dried powder, 30 to 34 portions of oligosaccharide, 3 to 5 portions of lactobacillus acidophilus powder, 15 to 20 portions of skimmed milk powder and 0.01 to 0.05 portion of vitamin.
In one embodiment, the vitamin comprises at least one of vitamin a, vitamin B, vitamin C, vitamin D, and vitamin E.
In one embodiment, the oligosaccharide comprises at least one of isomaltooligosaccharides, fructooligosaccharides, galactooligosaccharides, soy oligosaccharides, raffinose mixed milk sucrose, and xylooligosaccharides.
In one embodiment, the probiotic composition comprises the following components by weight:
5 parts of bifidobacterium lactis powder, 4 parts of bifidobacterium longum powder, 4 parts of bifidobacterium breve powder, 32 parts of oligosaccharide, 4 parts of lactobacillus acidophilus powder, 18 parts of skimmed milk powder and 0.03 part of vitamin.
The invention also provides a preparation method of the probiotic composition, which comprises the following steps:
Weighing 4-6 parts of bifidobacterium lactis powder freeze-dried powder, 3-5 parts of bifidobacterium longum powder freeze-dried powder, 4-6 parts of bifidobacterium breve powder freeze-dried powder, 3-5 parts of lactobacillus acidophilus powder freeze-dried powder, 15-20 parts of skim milk powder, 0.01-0.05 part of vitamin and 30-34 parts of oligosaccharide according to parts by weight;
Mixing the weighed raw materials to obtain a mixed material, adding water into the mixed material, and stirring at the temperature of 5-10 ℃ to obtain a mixed solution;
and (3) placing the mixed solution in a vacuum environment for standing, filtering and separating to dry and crush the separated solid material, and sealing the crushed solid material to obtain the probiotic composition.
In one embodiment, the drying temperature of the solid material is 30 ℃ to 42 ℃.
In one embodiment, the weight of the mixed material is 50 to 80 times of the weight of the water.
In one embodiment, the probiotic composition comprises viable bifidobacterium lactis powder greater than 10 10 CFU/g, viable bifidobacterium longum powder greater than 10 10 CFU/g, viable bifidobacterium breve powder greater than 10 10 CFU/g, and viable lactobacillus acidophilus powder greater than 10 11 CFU/g.
The invention provides a probiotic composition and a preparation method thereof, wherein the probiotic composition comprises the following components in parts by weight: 4 to 6 portions of bifidobacterium lactis powder, 3 to 5 portions of bifidobacterium longum powder, 4 to 6 portions of bifidobacterium breve powder freeze-dried powder, 30 to 34 portions of oligosaccharide, 3 to 5 portions of lactobacillus acidophilus powder, 15 to 20 portions of skimmed milk powder and 0.01 to 0.05 portion of vitamin; the various bifidobacteria in the probiotic composition can promote the digestion of food in the large intestine, the lactobacillus can promote the digestion of food in the small intestine, and the bifidobacteria and the lactobacillus can inhibit the growth of harmful bacteria, thereby reducing the generation of endotoxin in a human body and lightening the detoxification burden of the liver; in addition, the bifidobacteria and the lactobacillus acidophilus can promote the absorption of vitamins by human bodies, namely the probiotics can supplement the vitamins required by the human bodies; in addition, the probiotic composition contains oligosaccharide, so that the content of bifidobacterium can be increased, and the probiotic composition is suitable for being taken by diabetics.
Detailed Description
it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
first, a brief overview of the preparation of probiotic compositions provided by the examples of the present invention is provided.
The preparation method of the probiotic composition provided by the embodiment of the invention comprises the following steps:
(1) weighing 4-6 parts of bifidobacterium lactis powder freeze-dried powder, 3-5 parts of bifidobacterium longum powder freeze-dried powder, 4-6 parts of bifidobacterium breve powder freeze-dried powder, 3-5 parts of lactobacillus acidophilus powder freeze-dried powder, 15-20 parts of skim milk powder, 0.01-0.05 part of vitamin and 30-34 parts of oligosaccharide according to parts by weight, wherein the vitamin comprises at least one of vitamin A, vitamin B, vitamin C, vitamin D and vitamin E, and the oligosaccharide comprises at least one of isomaltooligosaccharide, fructooligosaccharide, galactooligosaccharide, soybean oligosaccharide, raffinose mixed lactosucrose and xylooligosaccharide;
(2) Mixing the weighed raw materials to obtain a mixed material, adding water into the mixed material, and stirring at the temperature of 5-10 ℃ to obtain a mixed solution, wherein the weight of the mixed material is 50-80 times that of the water;
(3) stirring the mixed materials mixed with water for 15-30 min, standing the mixed solution for 15-20 min, filtering and separating to obtain a solid material, drying and crushing the separated solid material to obtain particles with the particle size of less than 0.2mm, sealing the crushed solid material to obtain the probiotic composition, wherein the drying temperature of the crushed solid material is controlled at 30-42 ℃.
The probiotic composition prepared by the preparation method comprises active bifidobacterium lactis of more than 10 10 CFU/g, active bifidobacterium longum of more than 10 10 CFU/g, active bifidobacterium breve of more than 10 10 CFU/g and active lactobacillus acidophilus of more than 10 11 CFU/g.
According to the probiotic composition produced by the production method, various types of bifidobacteria in the probiotic composition can promote digestion of food in the large intestine, the lactobacillus acidophilus can promote digestion of food in the small intestine, and the bifidobacteria and the lactobacillus acidophilus can inhibit growth of harmful bacteria, so that the generation of endotoxin in a human body is reduced, and the detoxification burden of the liver is relieved; in addition, the bifidobacteria and the lactobacillus acidophilus can promote the absorption of vitamins by human bodies, namely the probiotics can supplement the vitamins required by the human bodies; in addition, the probiotic composition contains oligosaccharide, so that the content of bifidobacterium can be increased, and the probiotic composition is suitable for being taken by diabetics.
example 1
Step 1: weighing the following raw materials in parts by weight: 5 parts of bifidobacterium lactis stem dry frozen powder, 4 parts of bifidobacterium longum stem dry frozen powder, 4 parts of bifidobacterium breve stem dry frozen powder, 10 parts of isomaltooligosaccharide, 11 parts of fructo-oligosaccharide, 11 parts of galacto-oligosaccharide, 4 parts of lactobacillus acidophilus stem dry frozen powder, 18 parts of skimmed milk powder, 0.02 part of vitamin E and 0.01 part of vitamin B, wherein one part is unit weight which can be 1g, 10g, 100g, 1000g and the like, and the unit weight is not limited;
Step 2: mixing the weighed raw materials, mixing the raw materials by a two-dimensional or three-dimensional mixer to obtain a mixed material, adding purified water into the mixed material, wherein the weight ratio of the mixed material to the water is 75:1, and stirring the mixed material at the temperature of 5 ℃;
And step 3: and after stirring for 25min, stopping stirring, standing the mixed solution for 20min for filtering and separating to obtain a solid material, drying the solid material at 30 ℃ for 3h to obtain a dried solid material, crushing the solid material to obtain a particulate solid material with the particle size of less than 0.2mm, and finally sealing the particulate solid material to obtain the probiotic composition.
The probiotic composition prepared in this example had a content of viable bifidobacterium lactis of 1.1 × 10 10 CFU/g, a content of viable bifidobacterium longum of 1.3 × 10 10 CFU/g, a content of viable bifidobacterium breve of 1.2 × 10 10 CFU/g, and a content of viable lactobacillus acidophilus of 1.21 × 10 11 CFU/g.
Example 2
Step 1: weighing the following raw materials in parts by weight: 4 parts of bifidobacterium lactis stem dry frozen powder, 5 parts of bifidobacterium longum stem dry frozen powder, 6 parts of bifidobacterium breve stem dry frozen powder, 8 parts of soybean oligosaccharide, 12 parts of raffinose mixed milk cane sugar, 14 parts of xylo-oligosaccharide, 4 parts of lactobacillus acidophilus stem dry frozen powder, 18 parts of skimmed milk powder, 0.01 part of vitamin C and 0.01 part of vitamin D, wherein one part is unit weight which can be 1g, 10g, 100g, 1000g and the like, and the unit weight is not limited;
Step 2: mixing the weighed raw materials, mixing the raw materials by a two-dimensional or three-dimensional mixer to obtain a mixed material, adding purified water into the mixed material, wherein the weight ratio of the mixed material to the water is 70:1, and stirring the mixed material at the temperature of 8 ℃;
and step 3: and after stirring for 22min, stopping stirring, standing the mixed solution for 18min for filtering and separating to obtain a solid material, drying the solid material at 35 ℃ for 3.5h to obtain a dried solid material, crushing the solid material to obtain a particulate solid material with the particle size of less than 0.15mm, and finally sealing the particulate solid material to obtain the probiotic composition.
The probiotic composition prepared in this example had a content of viable bifidobacterium lactis of 1.1 × 10 10 CFU/g, a content of viable bifidobacterium longum of 1.2 × 10 10 CFU/g, a content of viable bifidobacterium breve of 1.18 × 10 10 CFU/g, and a content of viable lactobacillus acidophilus of 1.15 × 10 11 CFU/g.
Example 3
Step 1: weighing the following raw materials in parts by weight: 4 parts of bifidobacterium lactis stem dry frozen powder, 3 parts of bifidobacterium longum stem dry frozen powder, 3 parts of bifidobacterium breve stem dry frozen powder, 11 parts of isomaltooligosaccharide, 15 parts of fructo-oligosaccharide, 6 parts of xylo-oligosaccharide, 4 parts of lactobacillus acidophilus stem dry frozen powder, 18 parts of skimmed milk powder, 0.02 part of vitamin B and 0.03 part of vitamin C, wherein one part is unit weight which can be 1g, 10g, 100g, 1000g and the like, and the unit weight is not limited;
Step 2: mixing the weighed raw materials, mixing the raw materials by a two-dimensional or three-dimensional mixer to obtain a mixed material, adding purified water into the mixed material, wherein the weight ratio of the mixed material to the water is 60:1, and stirring the mixed material at 10 ℃;
and step 3: and after stirring for 18min, stopping stirring, standing the mixed solution for 16min for filtering and separating to obtain a solid material, drying the solid material at 40 ℃ for 4h to obtain a dried solid material, crushing the solid material to obtain a particulate solid material with the particle size of less than 0.18mm, and finally sealing the particulate solid material to obtain the probiotic composition.
The probiotic composition prepared in this example had a content of viable bifidobacterium lactis of 1.12 × 10 10 CFU/g, a content of viable bifidobacterium longum of 1.1 × 10 10 CFU/g, a content of viable bifidobacterium breve of 1.1 × 10 10 CFU/g, and a content of viable lactobacillus acidophilus of 1.13 × 10 11 CFU/g.
example 4
Step 1: weighing the following raw materials in parts by weight: 4 parts of bifidobacterium lactis stem dry frozen powder, 4 parts of bifidobacterium longum stem dry frozen powder, 5 parts of bifidobacterium breve stem dry frozen powder, 5 parts of isomaltooligosaccharide, 6 parts of fructo-oligosaccharide, 3 parts of galacto-oligosaccharide, 8 parts of soybean oligosaccharide, 4 parts of raffinose mixed milk sucrose, 4 parts of xylo-oligosaccharide, 5 parts of lactobacillus acidophilus stem dry frozen powder, 17 parts of skimmed milk powder, 0.02 part of vitamin A and 0.02 part of vitamin C, wherein one part is unit weight which can be 1g, 10g, 100g, 1000g and the like, and the unit weight is not limited;
Step 2: mixing the weighed raw materials, mixing the raw materials by a two-dimensional or three-dimensional mixer to obtain a mixed material, adding purified water into the mixed material, wherein the weight ratio of the mixed material to the water is 80:1, and stirring the mixed material at 6 ℃;
And step 3: and after stirring for 30min, stopping stirring, standing the mixed solution for 16min for filtering and separating to obtain a solid material, drying the solid material at 42 ℃ for 3.5h to obtain a dried solid material, crushing the solid material to obtain a particulate solid material with the particle size of less than 0.2mm, and finally sealing the particulate solid material to obtain the probiotic composition.
The probiotic composition prepared in the example has a content of active bifidobacterium lactis of 1.04 × 10 10 CFU/g, a content of active bifidobacterium longum of 1.05 × 10 10 CFU/g, a content of active bifidobacterium breve of 1.01 × 0 10 CFU/g, and a content of active lactobacillus acidophilus of 1.15 × 10 11 CFU/g.
example 5
Step 1: weighing the following raw materials in parts by weight: 6 parts of bifidobacterium lactis stem dry frozen powder, 4 parts of bifidobacterium longum stem dry frozen powder, 6 parts of bifidobacterium breve stem dry frozen powder, 15 parts of isomaltooligosaccharide, 16 parts of fructo-oligosaccharide, 4 parts of lactobacillus acidophilus stem dry frozen powder, 20 parts of skim milk powder, 0.005 part of vitamin A, 0.005 part of vitamin B, 0.08 part of vitamin C and 0.05 part of vitamin D, wherein one part is unit weight which can be 1g, 10g, 100g, 1000g and the like, and the unit weight is not limited;
Step 2: mixing the weighed raw materials, mixing the raw materials by a two-dimensional or three-dimensional mixer to obtain a mixed material, adding purified water into the mixed material, wherein the weight ratio of the mixed material to the water is 55:1, and stirring the mixed material at 7 ℃;
and step 3: and after stirring for 28min, stopping stirring, standing the mixed solution for 16min for filtering and separating to obtain a solid material, drying the solid material at 38 ℃ for 3h to obtain a dried solid material, crushing the solid material to obtain a particulate solid material with the particle size of less than 0.2mm, and finally sealing the particulate solid material to obtain the probiotic composition.
The probiotic composition prepared in this example had a content of viable bifidobacterium lactis of 1.2 × 10 10 CFU/g, a content of viable bifidobacterium longum of 1.1 × 10 10 CFU/g, a content of viable bifidobacterium breve of 1.1 × 10 10 CFU/g, and a content of viable lactobacillus acidophilus of 1.05 × 10 11 CFU/g.
it should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalents and equivalent flow changes made by using the contents of the present specification, or applied directly or indirectly to other related technical fields are also included in the scope of the present invention.

Claims (8)

1. A probiotic composition for regulating intestinal flora, comprising the following components in parts by weight:
4 to 6 portions of bifidobacterium lactis powder, 3 to 5 portions of bifidobacterium longum powder, 4 to 6 portions of bifidobacterium breve powder freeze-dried powder, 30 to 34 portions of oligosaccharide, 3 to 5 portions of lactobacillus acidophilus powder, 15 to 20 portions of skimmed milk powder and 0.01 to 0.05 portion of vitamin.
2. The probiotic composition of claim 1, wherein the vitamins include at least one of vitamin a, vitamin B, vitamin C, vitamin D, and vitamin E.
3. The probiotic composition of claim 1, wherein the oligosaccharides comprise at least one of isomaltooligosaccharides, fructooligosaccharides, galactooligosaccharides, soy oligosaccharides, raffinose mixed milk sucrose, xylooligosaccharides.
4. The probiotic composition according to claim 1, characterized in that it comprises the following components in weight:
5 parts of bifidobacterium lactis powder, 4 parts of bifidobacterium longum powder, 4 parts of bifidobacterium breve powder, 32 parts of oligosaccharide, 4 parts of lactobacillus acidophilus powder, 18 parts of skimmed milk powder and 0.03 part of vitamin.
5. a method for preparing a probiotic composition, wherein the method for preparing the probiotic composition comprises the following steps:
weighing 4-6 parts of bifidobacterium lactis powder freeze-dried powder, 3-5 parts of bifidobacterium longum powder freeze-dried powder, 4-6 parts of bifidobacterium breve powder freeze-dried powder, 3-5 parts of lactobacillus acidophilus powder freeze-dried powder, 15-20 parts of skim milk powder, 0.01-0.05 part of vitamin and 30-34 parts of oligosaccharide according to parts by weight;
Mixing the weighed raw materials to obtain a mixed material, adding water into the mixed material, and stirring at the temperature of 5-10 ℃ to obtain a mixed solution;
And standing, filtering and separating the mixed solution to dry and crush the separated solid material, and sealing the crushed solid material to obtain the probiotic composition.
6. The method of preparing the probiotic composition according to claim 5, characterized in that the drying temperature of the solid material is between 30 ℃ and 42 ℃.
7. The method of preparing the probiotic composition of claim 5, wherein the weight of the blend is 50 to 80 times the weight of the water.
8. the method of preparing the probiotic composition according to any one of claims 5 to 7, wherein the probiotic composition comprises Bifidobacterium lactis greater than 10 10 CFU/g, Bifidobacterium longum greater than 10 10 CFU/g, Bifidobacterium breve greater than 10 10 CFU/g, and Lactobacillus acidophilus greater than 10 11 CFU/g.
CN201910885310.9A 2019-09-18 2019-09-18 Probiotic composition and preparation method thereof Pending CN110547469A (en)

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Citations (7)

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Publication number Priority date Publication date Assignee Title
CN101099749A (en) * 2007-07-25 2008-01-09 上海交大昂立股份有限公司 Composition containing lactobacillus acidophilus and bidifobacterium longum and application thereof
CN105707560A (en) * 2014-12-01 2016-06-29 哈尔滨墨医生物技术有限公司 Hericium erinaceus probiotics powder and production method thereof
CN105724849A (en) * 2014-12-10 2016-07-06 黑龙江众生生物工程有限公司 Spirulina and probiotic powder and production method thereof
CN106889414A (en) * 2017-02-17 2017-06-27 汉臣氏（沈阳）儿童制品有限公司 Double bacterium solid hawthorn beverages
CN109288063A (en) * 2018-09-14 2019-02-01 安吉艾格赛思生物科技有限公司 A kind of multi-joint Cernevit-12 and preparation method thereof being proliferated beneficial bacteria of intestinal tract
CN109329419A (en) * 2018-08-28 2019-02-15 吉林标普生物科技有限公司 A kind of functional probiotics solid beverage and preparation method thereof for being able to maintain that intestinal health and improving immunity
CN109430666A (en) * 2018-10-15 2019-03-08 威海养经堂医药科技有限公司 A kind of Freeze-dry Powder of Probioctics solid beverage

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099749A (en) * 2007-07-25 2008-01-09 上海交大昂立股份有限公司 Composition containing lactobacillus acidophilus and bidifobacterium longum and application thereof
CN105707560A (en) * 2014-12-01 2016-06-29 哈尔滨墨医生物技术有限公司 Hericium erinaceus probiotics powder and production method thereof
CN105724849A (en) * 2014-12-10 2016-07-06 黑龙江众生生物工程有限公司 Spirulina and probiotic powder and production method thereof
CN106889414A (en) * 2017-02-17 2017-06-27 汉臣氏（沈阳）儿童制品有限公司 Double bacterium solid hawthorn beverages
CN109329419A (en) * 2018-08-28 2019-02-15 吉林标普生物科技有限公司 A kind of functional probiotics solid beverage and preparation method thereof for being able to maintain that intestinal health and improving immunity
CN109288063A (en) * 2018-09-14 2019-02-01 安吉艾格赛思生物科技有限公司 A kind of multi-joint Cernevit-12 and preparation method thereof being proliferated beneficial bacteria of intestinal tract
CN109430666A (en) * 2018-10-15 2019-03-08 威海养经堂医药科技有限公司 A kind of Freeze-dry Powder of Probioctics solid beverage

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Application publication date: 20191210