CN110531023A - A kind of thin-layer chromatography analyzer - Google Patents
A kind of thin-layer chromatography analyzer Download PDFInfo
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- CN110531023A CN110531023A CN201910949902.2A CN201910949902A CN110531023A CN 110531023 A CN110531023 A CN 110531023A CN 201910949902 A CN201910949902 A CN 201910949902A CN 110531023 A CN110531023 A CN 110531023A
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- insulating box
- fixed frame
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- 238000004809 thin layer chromatography Methods 0.000 title claims abstract description 16
- 239000002904 solvent Substances 0.000 claims description 25
- 230000008878 coupling Effects 0.000 claims description 24
- 238000010168 coupling process Methods 0.000 claims description 24
- 238000005859 coupling reaction Methods 0.000 claims description 24
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 238000004587 chromatography analysis Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000001035 drying Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 13
- 238000010586 diagram Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 230000005611 electricity Effects 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012916 chromogenic reagent Substances 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
The invention discloses a kind of thin-layer chromatography analyzer, the functions such as highly integrated point sample, drying, expansion, colour developing are in one；It mainly include insulating box, fixed frame, rack for test tube, pillar and diaphragm pump, ultraviolet lamp and incandescent lamp are fixedly installed at the top of the insulating box, bottom is fixedly connected with fixed frame, air-heater is fixed by blower bracket in fixed frame bottom, the rack for test tube is fixedly mounted on slide unit 1, capillary and test tube are placed on rack for test tube, the pillar is fixed in insulating box, slide unit group is installed on pillar, electronic clamping jaw is installed for clamping capillary on slide unit 3, the diaphragm pump is fixedly mounted on constant temperature chamber interior wall, one end is tightly connected suction hose and draws color developing agent from color developing agent cup, the other end is tightly connected outlet pipe, color developing agent is uniformly sprayed from outlet pipe with Sprayable.The present invention has many advantages, such as small in size, easy to operate, high-efficient, high safety.
Description
Technical field
The present invention relates to medical Instrument technology field, specially a kind of thin-layer chromatography analyzer.
Background technique
Thin-layered chromatography is a kind of common micro and quick chromatography, it have it is time-consuming less, high sensitivity, operation side
Just the advantages that, thus in chemical industry, medicine and other fields are used widely.Its main operational principle is (glass plate, modeling on support plate
Flitch etc. has some strength and chemically inert surface plate) adsorbent is uniformly painted into a thin layer, then drawn and tried by capillary
Sample solution and by its accurate place on the straight line apart from 1-2 centimetres of support plate one end.Support plate is put into after the solvent is volatilized
In pre-saturated expansion cylinder, due to capillarity, sample solution is advanced slowly on lamellae, after advancing to certain distance,
Support plate is taken out, sample component is separated from each other the spot to form different distance because of movement speed difference.Finally use ultraviolet lamp
Irradiation or color developing agent detect spot, are determined according to Rf value (Retardation factor, Rf) the ingredient of sample solution
Amount analysis.
The result of thin-layer chromatographic analysis is mainly influenced by factors such as temperature, humidity, the accuracys of point sample.Temperature influences each
Steam rates in the evaporation degree and expansion cylinder of organic solvent, and then the solubility of aqueous solvent two-phase is influenced, change solvent
Ratio；Humidity mainly influences the adsorption capacity of lamellae, can achieve different adsorption equilibriums under different relative humidity；
Point sample amount, which crosses senior general, reduces separative efficiency, and the printing operation of mistake can cause to damage to thin layer surface, greatly influence color
The quality of spectrum.
It is had the following problems in existing thin-layer chromatographic analysis operating method:
1. manual operation is limited to the experience and precision of operator, error will be inevitably resulted in, and entirely grasp
Make process to be in open space, the humidity and temperature of environment all will affect thin-layer chromatographic analysis result.
2. need to be with solvent close contact when manual operation, the volatilization of the poisonous and harmful solvent in part can be to the health of operator
It causes damages.
3. existing thin-layer chromatography analyzer device (such as: auto sample applicator, Automatic-expanding instrument, spraying system) is mostly will be thin
The a certain step of layer chromatography process realizes automation, be still required operator support plate is taken out from instrument be placed in again it is another
In one instrument, this process, which is susceptible to environment temperature and the influence of humidity, causes the chromatography obtained second-rate, and needs to grasp
Author accompanies in the whole journey, cumbersome, low efficiency.
To solve the above problems, a kind of thin-layer chromatography analyzer is devised, highly integrated point sample, drying, expansion, colour developing etc.
Function realizes that thin-layer chromatographic analysis operation is full-automatic, has small in size, easy to operate, high-efficient, high safety etc. in one
Advantage.
Summary of the invention
The object of the present invention is to provide a kind of thin-layer chromatography analyzers, to solve the problems, such as to propose in above-mentioned background.
To achieve the above object, the invention provides the following technical scheme: a kind of thin-layer chromatography analyzer, including insulating box,
Fixed frame, pillar and diaphragm pump, it is characterised in that: the insulating box right lateral surface is fixed with hold-down support, the hold-down support
It is hinged with offside door, it is hinged with hermatic door on the left of the insulating box, is fixedly installed with ultraviolet lamp and white heat at the top of the insulating box
Lamp, the insulating box bottom are fixedly connected with fixed frame, and air-heater is fixed by blower bracket in the fixed frame bottom, described
Fixed frame top is fixedly connected with sliding rail 4, is equipped with motor 5 on the right side of the sliding rail 4,5 output shaft of motor by shaft coupling 4 with
Screw rod 4 connects, and the screw rod 4 is equipped with slide unit 4, and the slide unit 4 is fixedly connected with bottom plate, is fixedly mounted on the bottom plate with teeth
Reversing box and motor 4, the bottom plate and swivel plate rotation connection are taken turns, is fixedly connected with the one of electrohydraulic cylinder on the swivel plate
End, the electrohydraulic cylinder other end are fixedly connected with support plate folder, and the pillar is fixed on insulating box bottom, the post top portion
Sliding rail 2 is fixedly mounted, motor 2 is installed on the left of the sliding rail 2,2 output shaft of motor is connect by shaft coupling 2 with screw rod 2,
The screw rod 2 is equipped with slide unit 2, and the slide unit 2 is fixedly connected with sliding rail 3, motor 3, the motor are equipped at the top of the sliding rail 3
3 output shafts are connect by shaft coupling 3 with screw rod 2, and the screw rod 3 is equipped with slide unit 3, and the slide unit 3 is fixedly connected with electrical clip
Pawl has by the fixed frame sliding rail 1 to be fixed on insulating box bottom, and described 1 one end of sliding rail is equipped with motor 1, and the motor 1 exports
Axis is connect by shaft coupling 1 with screw rod 1, and the screw rod 1 is equipped with slide unit 1, and the slide unit 1 is fixedly connected with rack for test tube, described
Diaphragm pump is fixedly mounted on constant temperature chamber interior wall.
Preferably, it is equipped with partition in the insulating box, humidifier, the insulating box top are fixed with inside the insulating box
It is fixed with surveillance mirror, the insulating box surface is fixed with temperature humidity display, and one jiao of affiliated insulating box has a rectangular channel, puts in slot
It is equipped with color developing agent cup.
Preferably, it being equipped with sliding slot among the fixed frame top layer, there are vacancy to be placed with expansion cylinder for the fixed frame left end,
The fixed frame left end is equipped with spring, and the expansion cylinder cover back-end is equipped with two holes, and there is the slot with support plate folder same widths in front end,
The expansion cylinder cap is tightly pressed against on fixed frame by spring, forms closed space with expansion cylinder.
Preferably, the air-heater and air hose are tightly connected, and the air hose is stretched out along the hole of fixed frame side.
Preferably, the support plate folder is equipped with scale, and support plate folder side is equipped with telescopic rod.
Preferably, the diaphragm pump left end is sealedly connected with outlet pipe, and the outlet pipe is fixed on partition by support of pipelines
On, tail end is vertically downward and the micropore of regular distribution, the right end of the diaphragm pump are sealedly connected with suction hose, the suction hose
It is pierced by from the hole of insulating box, end is protruded into color developing agent cup.
Preferably, the hold-down support, pillar, support of pipelines, spring, blower bracket it is each there are two.
Compared with prior art, the beneficial effects of the present invention are:
1, by point sample, expansion, drying and colour developing during thin-layer chromatographic analysis and etc. realize full-automatic operation, and it is high
Degree is integrated in insulating box, and the triviality of manual operation or simple function instrument step-by-step operation is effectively reduced, and has small in size, operation
Easy advantage.
2, whole operation process is all in closed insulating box, effectively avoids under varying environment temperature and humidity to analysis
Influence.Device is cooperated using high-precision slide unit and electronic clamping jaw, so that point sample spacing is accurate, constant area, point sample amount are accurate,
And the physical damnification caused by support plate is reduced, the accuracy of thin-layer chromatographic analysis is further improved, operates the chromatography obtained
Quality is high.
3, device is pressed from both sides using telescopic support plate, is suitable for plurality of specifications support plate.Capillary point sample is taken using electrical clip claw clip
Mode can separate simultaneously multi-solvents, and accomplish that a capillary draws a kind of solvent, avoid solvent from mixing and right
Analysis result has an impact.Internal air-heater avoids poisonous and harmful solvent volatilization and causes to human body while capable of accelerating air-dried
Injury has the advantages of high-efficient, high safety.
Detailed description of the invention
Fig. 1 is insulating box schematic diagram in the present invention；
Fig. 2 is internal structure leftschematic diagram in the present invention；
Fig. 3 is slide unit group schematic diagram in the present invention；
Fig. 4 is internal structure right side schematic view in the present invention；
Fig. 5 is fixed frame schematic diagram in the present invention；
Fig. 6 is fixed frame schematic cross-sectional view in the present invention；
Fig. 7 is that support plate presss from both sides schematic diagram in the present invention；
Fig. 8 is that cylinder cap schematic diagram is unfolded in the present invention；
Fig. 9 is swivel plate schematic diagram in the present invention；
Figure 10 is expansion cylinder schematic diagram in the present invention；
In figure: 7-13-hold-down support of telescopic rod 10-insulating box, 11-surveillance mirror, 12-offside door, 14-colour developings
Agent cup 15-temperature humidity display, 21-hermatic door, 22-partition, 23-ultraviolet lamp, 24-humidifier, 25-incandescent lamp
30-motor 1 31-screw rod, 1 32-bearing block, 1 33-slide unit, 1 34-sliding rail, 1 35-capillary, 36-rack for test tubes
37-2 313-slide units of test tube 38-motor, 2 39-shaft coupling, 2 310-screw rod, 2 311-bearing block, 2 312-sliding rail 2
314-3 318-slide units of motor 3 315-shaft coupling, 3 316-screw rod, 3 317-sliding rail 3 319-electronic clamping jaw 320-
50-spring of pillar 321-shaft coupling, 1 40-support of pipelines, 41-suction hose, 42-diaphragm pump, 43-outlet pipe 51-
52-gear wheel reversing of cylinder cap case, 53-motor, 4 54-electrohydraulic cylinder, 55-support plate, 56-support plate folder, 57-rotation is unfolded
Plate 58-bottom plate, 59-fixed frame, 60-expansion cylinder, 61-screw rod, 4 62-sliding rail, 4 63-air hose, 64-blower bracket
65-4 68-motors of slide unit 466-air-heater, 67-shaft coupling 5.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, to the technical solution in the present invention carry out it is clear, completely retouch
It states, it is clear that described embodiments are only a part of the embodiments of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, all embodiments obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Please refer to Fig. 1 to Figure 10, a kind of technical solution provided by the invention: including insulating box, fixed frame, pillar and diaphragm
Pump, it is characterised in that: the insulating box right lateral surface is fixed with hold-down support, and the hold-down support and offside door are hinged, described
It is hinged with hermatic door on the left of insulating box, partition is equipped in the insulating box, is fixedly installed with humidifier inside the insulating box, institute
It states and is fixed with surveillance mirror at the top of insulating box, the insulating box surface is fixed with temperature humidity display, and one jiao of affiliated insulating box has one
Rectangular channel is placed with color developing agent cup in slot, is fixedly installed with ultraviolet lamp and incandescent lamp, the insulating box bottom at the top of the insulating box
Portion is fixedly connected with fixed frame, and air-heater is fixed by blower bracket in the fixed frame bottom, and the air-heater and air hose are close
Envelope connection, the air hose are stretched out along the hole of fixed frame side, and the fixed frame top is fixedly connected with sliding rail 4,4 right side of sliding rail
Motor 5 is installed, 5 output shaft of motor is connect by shaft coupling 4 with screw rod 4, and the screw rod 4 is equipped with slide unit 4, the cunning
Platform 4 is fixedly connected with bottom plate, and gear wheel reversing case and motor 4, the bottom plate and swivel plate rotation connection are equipped on the bottom plate,
One end of electrohydraulic cylinder is fixedly connected on the swivel plate, the electrohydraulic cylinder other end is fixedly connected with support plate folder, institute
Support plate folder is stated, scale, the support plate folder are equipped with, side is equipped with telescopic rod, and the pillar is fixed on insulating box bottom, the branch
Sliding rail 2 is fixedly mounted in column top, and motor 2 is equipped on the left of the sliding rail 2, and 2 output shaft of motor passes through shaft coupling 2 and screw rod
2 connections, the screw rod 2 are equipped with slide unit 2, and the slide unit 2 is fixedly connected with sliding rail 3, motor 3, institute are equipped at the top of the sliding rail 3
It states 3 output shaft of motor to connect by shaft coupling 3 with screw rod 2, the screw rod 3 is equipped with slide unit 3, and the slide unit 3 is fixedly connected with
Electronic clamping jaw has sliding rail 1 to be fixed on insulating box bottom by the fixed frame, and described 1 one end of sliding rail is equipped with motor 1, the electricity
1 output shaft of machine is connect by shaft coupling 1 with screw rod 1, and the screw rod 1 is equipped with slide unit 1, and the slide unit 1 is fixedly connected with test tube
Frame, the diaphragm pump are fixedly mounted on constant temperature chamber interior wall, and the diaphragm pump left end is sealedly connected with outlet pipe, the outlet pipe by
Support of pipelines is fixed on partition, and tail end is vertically downward and the micropore of regular distribution, the right end of the diaphragm pump are tightly connected
There is suction hose, the suction hose is pierced by from the hole of insulating box, and end is protruded into color developing agent cup.
Working principle: it when the thin-layer chromatography analyzer works, is first turned on hermatic door (21) and takes out expansion cylinder (60),
Bottom is shifted on the left of fixed frame (59) after entering appropriate solvent, is closed hermatic door (21).Under original state, on fixed frame (59)
Expansion cylinder cap (51) is tightly bonded under the action of spring (50) with fixed frame (59), and closed with expansion cylinder (60) formation one
Space is saturated solvent sufficiently in expansion cylinder (60).It opens offside door (12), swivel plate (57) is in horizontal position at this time
It sets, support plate (55) is put to support plate folder (56), top alignment is tightly blocked support plate (55) by telescopic rod (7), is closed right
Humidifier (24) starts side door (12) afterwards, and when the interior humidity of insulating box (10) reaches specified numerical value, humidifier (24) is closed.Then
Motor 5 (68) starting drives screw rod 4 (61) rotation by shaft coupling 4 (67), to keep slide unit 4 (65) straight along sliding rail 4 (62)
Line is mobile.Slide unit 4 (65) is mobile with the bottom plate (58) of connection fixed thereto, while the swivel plate (57) on bottom plate (58), load
Plate presss from both sides (56) and support plate (55) synchronizing moving.After being moved to designated position, motor 5 (68) is closed, and motor 1 (30) starting passes through
Shaft coupling 1 (321) drives screw rod 1 (31) rotation, and slide unit 1 (33) is made to move linearly along sliding rail 1 (34).Slide unit 1 (33) drives
Motor 1 (30) is closed after the rack for test tube (36) of connection fixed thereto is moved to designated position.Motor 2 (38) starting, passes through shaft coupling
Device 2 (39) drives screw rod 2 (310) rotation, so that slide unit 2 (313) is moved linearly along sliding rail 2 (312), when electronic clamping jaw (319)
Motor 2 (38) is closed behind designated position on rack for test tube (36).Motor 3 (314) starting drives silk by shaft coupling 3 (315)
Bar 3 (316) rotation, makes slide unit 3 (318) move linearly along sliding rail 3 (317).Slide unit 3 (318) drives under electronic clamping jaw (319)
It after being down to designated position, clamps capillary (35), subsequent slide unit 3 (318) drives electronic clamping jaw (319) to rise certain distance, electricity
Machine 3 (314) is closed.Then motor 1 (30) and motor 2 (38) are mobile with slide unit 1 (33) and slide unit 2 (313) respectively, work as target
Test tube (37) is in motor 1 (30) and motor 2 (38) when immediately below electronic clamping jaw (319) and closes.Motor 3 (314) starting, it is sliding
Platform 3 (318) toward moving down, protrudes into capillary (35) and draws solvent in target test tube (37), drawn with electronic clamping jaw (319)
Cheng Hou, motor 3 (314) is closed after slide unit 3 (318) drives electronic clamping jaw (319) to rise to designated position.Motor 2 (38) starting,
Make slide unit 2 (313) with electronic clamping jaw (319) horizontal shifting to support plate (55) surface.Then, motor 2 (38) and motor 3
(314) cooperate, control electronic clamping jaw (319) and move up and down, make capillary (35) support plate (55) as desired on
Carry out accurately point sample.After the completion of point sample, motor 1 (30), motor 2 (38) and motor 3 (314) difference Mobile Slide 1 (33) are slided
It is in situ that capillary (35) is put back to rack for test tube (36) by platform 2 (313) and slide unit 3 (318).To carry out multi-solvents in support plate (55)
Aforesaid operations are repeated several times in point sample.Air-heater (66) starting, hot wind, which is blowed to support plate (55), by air hose (63) makes solvent
Accelerate moisture evaporation, is then turned off air-heater (66).Motor 5 (68) starting drives bottom plate (58) to be moved to by slide unit 4 (65)
The left end of fixed frame (59) sliding slot, bottom plate (58) are moved to the left while can push to the left expansion cylinder cap (51), be unfolded at this time
Cylinder cap (51) forms a rectangular recess at the top of expansion cylinder (60).It closes motor 5 (68) and starts motor 4 (53) afterwards, pass through tooth
After swivel plate (57) is rotated by 90 ° by wheel reversing box (52), close motor 4 (53).Then electrohydraulic cylinder (54) push down on
Support plate folder (56) and support plate (55) protrude into expansion cylinder (60), and support plate (55) bottom is contacted with expansion cylinder (60) internal solvent at this time,
Support plate folder (56) top is just overlapped with rectangular channel, to form closed space with expansion cylinder (60), carries out the expansion of solvent.
After the completion of expansion, electrohydraulic cylinder (54) drives support plate folder (56) and support plate (55) to retract, and motor 4 (53) starting passes through gear
Reversing box (52) closes motor 4 (53) after rotating swivel plate (57) to horizontal position.Motor 5 (68) starting, passes through slide unit 4
(65) mobile with bottom plate (58), until support plate (55) is in air hose (63) outlet port, closing motor 5 (68) is unfolded at this time
Cylinder cap (51) automatically returns to and fixed frame (59) bonding position in the case where spring (50) act on.Air-heater (66) starting, passes through air hose
(63) support plate (55) is dried in blowout hot wind.Air-heater (66) are closed after the completion of dry, starts motor 5 (68), passes through cunning
Platform 4 (65) moves linearly to the right with bottom plate (58), if sample solvent needs chromogenic reagent, is moved to out in support plate (55)
When below water pipe (43) tail end, diaphragm pump (42) draws color developing agent by suction hose (41) from color developing agent cup (14), then from out
Water pipe (43) tail end is sprayed in spraying form, and slide unit 4 (65) remains a constant speed linear movement, so color developing agent can be uniformly sprayed on
On support plate (55), after support plate (55) passes through below outlet pipe (43) completely, close diaphragm pump (42).Slide unit 4 (65) is moved to
Motor 5 (68) are closed when fixed frame (59) sliding slot right end.It opens ultraviolet lamp (23), observes support plate (55) by surveillance mirror (11)
Result is unfolded in upper solvent.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And deformation, the scope of the present invention are limited by the appended claims and its equivalent.
Claims (8)
1. a kind of thin-layer chromatography analyzer, including insulating box (10), fixed frame (59), pillar (320) and diaphragm pump (42), special
Sign is: insulating box (10) right lateral surface is fixed with hold-down support (13), the hold-down support (13) and offside door (12)
Hingedly, it is hinged with hermatic door (21) on the left of the insulating box (10), is fixedly installed with ultraviolet lamp at the top of the insulating box (10)
(23) it is fixedly connected with fixed frame (59) with incandescent lamp (25), insulating box (10) bottom, fixed frame (59) bottom is logical
Cross blower bracket (64) air-heater (66) are fixed, fixed frame (59) top is fixedly connected with sliding rail 4 (62), the sliding rail 4
(62) right side is equipped with motor 5 (68), and motor 5 (68) output shaft is connect by shaft coupling 4 (67) with screw rod 4 (61), institute
It states screw rod 4 (61) and slide unit 4 (65) is installed, the slide unit 4 (65) is fixedly connected with bottom plate (58), fixed on the bottom plate (58)
Gear wheel reversing case (52) and motor 4 (53), the bottom plate (58) and swivel plate (57) rotation connection, the swivel plate are installed
(57) one end of electrohydraulic cylinder (54), electrohydraulic cylinder (54) other end and the fixed company of support plate folder (56) are fixedly connected on
It connects, the pillar (320) is fixed on insulating box (10) bottom, is fixedly installed with sliding rail 2 (312), institute at the top of the pillar (320)
It states and motor 2 (38) is installed on the left of sliding rail 2 (312), motor 2 (38) output shaft passes through shaft coupling 2 (39) and screw rod 2
(310) it connecting, slide unit 2 (313) is installed on the screw rod 2 (310), the slide unit 2 (313) is fixedly connected with sliding rail 3 (317),
Motor 3 (314) is installed, motor 3 (314) output shaft passes through shaft coupling 3 (315) and screw rod at the top of the sliding rail 3 (317)
2 (310) connection, the screw rod 3 (316) are equipped with slide unit 3 (318), and the slide unit 3 (318) is fixedly connected with electronic clamping jaw
(319), sliding rail 1 (34) is fixed on insulating box (10) bottom by the fixed frame (59), described sliding rail 1 (34) one end is equipped with
Motor 1 (30), motor 1 (30) output shaft is connect by shaft coupling 1 (321) with screw rod 1 (31), on the screw rod 1 (31)
Slide unit 1 (33) is installed, the slide unit 1 (33) is fixedly connected with rack for test tube (36), and the diaphragm pump (42) is fixedly mounted on perseverance
Incubator (10) inner wall.
2. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: be equipped in the insulating box (10)
Partition (22), the insulating box (10) is internal to be fixedly installed with humidifier (24), is fixed with observation at the top of the insulating box (10)
Mirror (11), insulating box (10) surface are fixed with temperature humidity display (15), and (10) one jiaos of affiliated insulating box has a rectangular channel,
Color developing agent cup (14) are placed in slot.
3. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: in fixed frame (59) top layer
Between be equipped with sliding slot, fixed frame (59) left end there are vacancy place expansion cylinder (60), fixed frame (59) left end is equipped with
Spring (50), the expansion cylinder cap (51) rear end are equipped with two holes, and there are the slot with support plate folder (56) same widths, the expansion in front end
Cylinder cap (51) is tightly pressed against on fixed frame (59) by spring (50), forms closed space with expansion cylinder (60).
4. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: the air-heater (66) and air hose
(63) it is tightly connected, the air hose (63) is stretched out along the hole of fixed frame (59) side.
5. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: the support plate folder (56) is equipped with
Scale, the support plate folder (56) side are equipped with telescopic rod (7).
6. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: diaphragm pump (42) left end is close
Envelope is connected with outlet pipe (43), and the outlet pipe (43) is fixed on partition (22) by support of pipelines (40), and tail end is vertically downward
And the micropore of regular distribution, the right end of the diaphragm pump (42) are sealedly connected with suction hose (41), the suction hose (41) from
The hole of insulating box (10) is pierced by, and end is protruded into color developing agent cup (14).
7. a kind of thin-layer chromatography analyzer according to claim 1, it is characterised in that: the hold-down support (13), branch
There are two column (320), support of pipelines (40), spring (50), blower bracket (64) are each.
8. a kind of method of full-automatic thin-layer chromatographic analysis, operating process feature the following steps are included:
S1: first taking out expansion cylinder (60), pours into and shifts bottom after appropriate solvent on the left of fixed frame (59), then by support plate
(55) it puts to support plate folder (56), support plate (55) is tightly blocked by telescopic rod (7), is then turned off hermatic door by top alignment
(21) and offside door (12), start humidifier (24), humidifier (24) closes when the interior humidity of insulating box (10) reaches specified numerical value
It closes；
S2: starting motor 5 (68) drives screw rod 4 (61) rotation by shaft coupling 4 (67), to make slide unit 4 (65) along sliding rail
4 (62) linear movement, while slide unit 4 (65) is with the swivel plate on the bottom plate (58) and bottom plate (58) of connection fixed thereto
(57), support plate folder (56) and support plate (55) synchronizing moving are then turned off motor 5 (68) to designated position；
S3: starting motor 1 (30) drives screw rod 1 (31) rotation by shaft coupling 1 (321), makes slide unit 1 (33) along sliding rail 1
(34) it moves linearly, while the rack for test tube (36) of connection fixed thereto being driven to be moved to designated position, be then turned off motor 1
(30)；
S4: starting motor 2 (38) drives screw rod 2 (310) rotation by shaft coupling 2 (39), makes slide unit 2 (313) along sliding rail 2
(312) it moves linearly, closes motor 2 (38) after electronic clamping jaw (319) is in designated position on rack for test tube (36).Start motor
3 (314) drive screw rod 3 (316) rotation by shaft coupling 3 (315), slide unit 3 (318) are made to move linearly along sliding rail 3 (317).
It after slide unit 3 (318) drives electronic clamping jaw (319) to drop to designated position, clamps capillary (35), subsequent slide unit 3 (318) drives
Electronic clamping jaw (319) rises certain distance, and motor 3 (314) is closed.Then motor 1 (30) and motor 2 (38) are respectively with slide unit
1 (33) and slide unit 2 (313) are mobile, close motor 1 (30) when target test tube (37) is in immediately below electronic clamping jaw (319)
With motor 2 (38).Start motor 3 (314), slide unit 3 (318) toward moving down, protrudes into capillary (35) with electronic clamping jaw (319)
Solvent is drawn in target test tube (37).After the completion of absorption, slide unit 3 (318) drives electronic clamping jaw (319) to rise to designated position,
It is then turned off motor 3 (314)；
S5: starting motor 2 (38) makes slide unit 2 (313) with electronic clamping jaw (319) horizontal shifting to support plate (55) surface.With
Afterwards, motor 2 (38) and motor 3 (314) cooperate, and control electronic clamping jaw (319) and move up and down, make capillary (35) In
Support plate (55) is upper as desired to carry out accurately point sample.After the completion of point sample, motor 1 (30), motor 2 (38) and motor 3 (314) point
Capillary (35) is put back to rack for test tube (36) original position by other Mobile Slide 1 (33), slide unit 2 (313) and slide unit 3 (318).To carry
Plate (55) carries out multi-solvents point sample, and aforesaid operations are repeated several times；
S6: starting air-heater (66), hot wind, which is blowed to support plate (55), by air hose (63) makes solvent accelerate moisture evaporation, then closes
Close air-heater (66)；
S7: starting motor 5 (68) is moved to the left end of fixed frame (59) sliding slot, bottom by slide unit 4 (65) drive bottom plate (58)
Plate (58) is moved to the left while can push to the left expansion cylinder cap (51), and cylinder cap (51) shape at the top of expansion cylinder (60) is unfolded at this time
At a rectangular recess.It closes motor 5 (68) and starts motor 4 (53) afterwards, revolved swivel plate (57) by gear wheel reversing case (52)
After turning 90 degrees, close motor 4 (53).Then electrohydraulic cylinder (54), which is pushed down on, protrudes into exhibition with support plate folder (56) and support plate (55)
Open in cylinder (60), support plate (55) bottom is contacted with expansion cylinder (60) internal solvent at this time, support plate folder (56) top just with rectangular channel
It is overlapped, to form closed space with expansion cylinder (60), carries out the expansion of solvent；
S8: electrohydraulic cylinder (54) drives support plate folder (56) and support plate (55) to retract, and motor 4 (53) starting passes through gear wheel reversing
Case (52) closes motor 4 (53) after rotating swivel plate (57) to horizontal position.Motor 5 (68) starting, passes through slide unit 4 (62) band
Bottom plate (58) it is mobile, until support plate (55) be in air hose (63) outlet port, cylinder cap is unfolded in closing motor 5 (68) at this time
(51) it is automatically returned to and fixed frame (59) bonding position under spring (50) effect；
S9: air-heater (66) starting blows out hot wind by air hose (63) and support plate (55) is dried.Heat is closed after the completion of dry
Blower (66)；
S10: starting motor 5 (68) is moved linearly to the right by slide unit (4) with bottom plate (58), if sample solvent needs to develop the color
Agent colour developing, then when support plate (55) is moved to below outlet pipe (43) tail end, diaphragm pump (42) passes through suction hose (41) from colour developing
Color developing agent is drawn in agent cup (14), then is sprayed from outlet pipe (43) tail end in spraying form, and slide unit 4 (65) remains a constant speed straight line
It is mobile, so color developing agent can be uniformly sprayed on support plate (55), after support plate (55) passes through below outlet pipe (43) completely, close
Diaphragm pump (42)；
S11: slide unit 4 (65) closes motor 5 (68) when being moved to fixed frame (59) sliding slot right end, opens ultraviolet lamp (23), leads to
Cross solvent expansion result in surveillance mirror (11) observation support plate (55).
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