CN207036761U - A kind of full-automatic point sample instrument - Google Patents
A kind of full-automatic point sample instrument Download PDFInfo
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- CN207036761U CN207036761U CN201720866447.6U CN201720866447U CN207036761U CN 207036761 U CN207036761 U CN 207036761U CN 201720866447 U CN201720866447 U CN 201720866447U CN 207036761 U CN207036761 U CN 207036761U
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Abstract
It the utility model is related to a kind of full-automatic point sample instrument, it is provided with the microsyringe and sample motion platform, target plate motion platform of three-dimensional cooperative motion, the full-automatic accurate point sample to target plate can not only be completed, and target plate after point sample is dried, and the repeatability of point sample can be improved, greatly improve operating efficiency.The utility model by programme-control automatic sampling, take matrix liquid, point sample, point matrix liquid, cleaning injector, dry the operation such as target plate, the accurate point sample of sample and reagent on target plate can not only be realized, syringe needle can be all cleaned after printing operation each time is completed, it ensure that the quality of last target plate sample spot, but also with dry function, full-automatic accurate point sample is really realized, MALDI-TOF-MS instrument can be coordinated to carry out the analysis and identification of sample.
Description
Technical field
Spot sample device is the utility model is related to, more specifically to a kind of full-automatic point sample instrument.
Background technology
The auto sample applicator of prior art, function is single, can only typically realize automatic point sample action, and for follow-up behaviour
Make, and the cleaning etc. of injector, it can not realize.
Mass spectrograph is that ion is separated according to mass-to-charge ratio difference using electromagnetic principles, and then the quality of measurement of species
With the scientific instrument of content.MALDI-TOF-MS instrument (MALDI-TOF) is mass spectrometric one
Branch, fast and accurately several samples can be analyzed and identified simultaneously, usual sample is first to be fixed on target plate
Target spot is re-fed into MALDI-TOF-MS instrument (MALDI-TOF) and is analyzed and identified.
But the operation that sample is fixed on target plate at present is usually to be completed by artificial, this working method is not only imitated
Rate is relatively low, and the not high result that can even influence last spectrometer analysis of the precision of point sample.
Moreover, the auto sample applicator of prior art can not coordinate MALDI-TOF-MS instrument to enter
The analysis and identification of row sample.
Utility model content
The purpose of this utility model is overcome the deficiencies in the prior art, there is provided one kind coordinates Matrix Assisted Laser Desorption electricity
From the full-automatic point sample instrument for the analysis and identification that time of-flight mass spectrometer (MALDI-TOF) carries out sample.
The technical solution of the utility model is as follows:
A kind of full-automatic point sample instrument, control microsyringe are laterally moved, carried out in vertical direction in the horizontal direction
Move up and down, control the sample motion platform with array sample to carry out lengthwise movement in the horizontal direction below microsyringe,
The target plate motion platform that control top surface is provided with target plate carries out lengthwise movement in the horizontal direction below microsyringe;It is micro enter
Sample device, sample motion platform, target plate motion platform carry out cooperative motion according to control instruction, and microsyringe is automatically performed clear
Washing lotion groove cleaned, be sampled in target sample point, is carried out taking matrix liquid in matrix liquid bath, is carried out point sample in target
With a matrix liquid.
Preferably, being provided with drier on the moving line of target plate motion platform, control target plate motion platform enters
After in the range of row drier, target plate is dried.
Preferably, setting workbench, portal frame is set on workbench, and microsyringe passes through sample introduction driving machine
Structure is connected with portal frame, and the transverse movement of horizontal direction and the up and down motion of vertical direction are carried out on portal frame;Sample motion
Platform is arranged on workbench by sample drive mechanism, below portal frame, carries out the lengthwise movement of horizontal direction;Target
Horizontal direction of the plate motion platform along workbench is located at the side of sample motion platform, and work is arranged on by target plate drive mechanism
Make on platform, carry out the lengthwise movement of horizontal direction.
Preferably, cleaning liquid bath, matrix liquid bath are arranged on workbench, control microsyringe moves to cleaning fluid
Groove, cleaned automatically;Microsyringe moves to matrix liquid bath, and point takes matrix liquid.
Preferably, sample introduction drive mechanism include be arranged on portal frame horizontal slide rail, be slidably arranged in horizontal slide rail
On transverse slider, drive the cross motor of transverse slider, and be arranged on the upright driving mechanism of transverse slider side.
Preferably, upright driving mechanism includes being connected to the first order upright slide rail of transverse slider side, slides setting
First order upright slide block, the vertical motor of the first order of driving first order upright slide block on first order upright slide rail, Yi Jishe
Put the second level upright slide rail on first order upright slide block, the second level being slidably arranged on the upright slide rail of the second level is slided vertically
Block, the vertical motor in the second level for driving second level upright slide block;Microsyringe is installed on the upright slide block of the second level.
Preferably, sample drive mechanism includes first longitudinal direction slide rail, driving slide rail is arranged on first longitudinal direction slide rail
The first longitudinal direction motor of sample motion platform;Target plate drive mechanism includes second longitudinal direction slide rail, driving slide rail is arranged on second and indulged
To the second longitudinal direction motor of the target plate motion platform on slide rail.
Preferably, respectively in transverse slider, first order upright slide block, second level upright slide block, sample motion platform, target
The default end point of travel of plate motion platform is respectively arranged with limit switch, when transverse slider, first order upright slide block, the second level
When upright slide block, sample motion platform, target plate motion platform move to the position of limit switch restriction, stop motion.
Preferably, drier includes drying seal cover board, vavuum pump, magnetic valve, when target plate moves to dry sealing
Below cover plate, dry seal cover board and surround sealing space with target plate motion platform, target plate is located in sealing space, and magnetic valve is beaten
Open, vacuum pump work, target plate is dried in vacuo.
Preferably, limit switch is mechanical limit switch or photo-electric limit switch.
The beneficial effects of the utility model are as follows:
The utility model proposes full automatic point sampling device, the microsyringe and sample motion of three-dimensional cooperative motion are provided with
Platform, target plate motion platform, the full-automatic accurate point sample to target plate can not only be completed, and target plate after point sample is done
It is dry, and the repeatability of point sample can be improved, greatly improve operating efficiency.
The utility model by programme-control automatic sampling, take matrix liquid, point sample, point matrix liquid, cleaning injector, drying
Target plate etc. operates, and can not only realize the accurate point sample of sample and reagent on target plate, after printing operation each time is completed
Will cleaning syringe needle, ensure that the quality of last target plate sample spot, but also with dry function, really realize it is complete from
The accurate point sample of dynamicization, MALDI-TOF-MS instrument can be coordinated to carry out the analysis and identification of sample.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is Fig. 1 rearview;
Fig. 3 is Fig. 1 left view;
Fig. 4 is Fig. 1 right view;
Fig. 5 is workflow diagram of the present utility model;
In figure:1 is microsyringe, and 2 be cross motor, and 3 be second longitudinal direction motor, and 4 be first longitudinal direction motor, and 5 be
The vertical motor of one-level, 6 be the vertical motor in the second level, and 7 be horizontal slide rail, and 8 be second longitudinal direction slide rail, and 9 be first longitudinal direction slide rail, 10
It is first order upright slide rail, 11 be second level upright slide rail, and 12 be cleaning liquid bath, and 13 be matrix liquid bath, and 14 be that sample motion is put down
Platform, 15 be target plate motion platform, and 16 be dry seal cover board, and 17 be target plate, and 18 be vavuum pump, and 19 be that cross spacing switchs, 20
It is target plate limit switch, 21 be sample limit switch, and 22 be the vertical limit switch of the first order, and 23, which be that the second level is spacing vertically, opens
Close, 24 be drive coordination band, and 25 be magnetic valve, and 26 be power supply, and 27 be control main frame, and 28 be transverse slider, and 29 be that the first order is erected
Straight sliding block, 30 be second level upright slide block, and 100 be workbench.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples.
Function existing for prior art is single, Matrix Assisted Laser Desorption can not be coordinated to ionize in order to solve for the utility model
Time of-flight mass spectrometer carries out the deficiency of the analysis and identification of sample, there is provided a kind of full-automatic point sample instrument, can not only realize
The full-automatic accurate point sample of sample and reagent on target plate, and target plate after point sample can be dried, greatly improve a little
The repeatability and point sample precision of sample, while greatly improve operating efficiency.
Full-automatic point sample instrument as shown in Figure 1, Figure 2, Figure 3, Figure 4, be provided with the microsyringe 1 of three-dimensional cooperative motion with
Sample motion platform 14, target plate motion platform 15, that is, microsyringe 1 is controlled to be laterally moved in the horizontal direction, vertical
Direction is moved up and down, control band array sample (for different types of sample liquid, up to hundreds of sample liquids can be set)
Sample motion platform 14 carries out lengthwise movement in the horizontal direction in the lower section of microsyringe 1, and control top surface is provided with target plate 17
Target plate motion platform 15 carries out lengthwise movement in the horizontal direction in the lower section of microsyringe 1;Microsyringe 1, sample motion are put down
Platform 14, target plate motion platform 15 carry out cooperative motion according to control instruction, and microsyringe 1 is automatically performed to be entered in cleaning liquid bath 12
Row cleaning, it is sampled in target sample point, take matrix liquid in matrix liquid bath 13, with contact or non-connect in target
The mode of touch carries out point sample with putting matrix liquid.Matrix liquid and sample liquid mix according to a certain percentage through drying with
Afterwards, matrix liquid Assisted Laser Desorption ionization time-of-flight mass spectrometer (MALDI-TOF) is placed into be analyzed and identified.
In the utility model, array sample is provided with sample motion platform 14, because microsyringe 1 can be in level
Direction is laterally moved, and sample motion platform 14 can carry out lengthwise movement in the horizontal direction, then microsyringe 1 and sample
The cooperative motion of motion platform 14, you can microsyringe 1 is aligned to each target sample of display sample exactly, then controlled
Microsyringe 1 is moved downward in vertical direction, you can is sampled.Similarly, can also be accurately enterprising in target plate 17
Row point sample, cleaned in cleaning liquid bath 12, carry out taking matrix liquid in matrix liquid bath 13, a matrix liquid is carried out on target plate 17.
In order to further increase function of the present utility model, and ensure the quality of the sample spot of target plate 17, moved in target plate
Drier is provided with the moving line of platform 15, it is right after control target plate motion platform 15 is dried in the range of mechanism
Target plate 17 is dried.
Workbench 100 is set in the utility model, portal frame is set on workbench 100, and microsyringe 1 passes through
Sample introduction drive mechanism is connected with portal frame, and the transverse movement of horizontal direction and the fortune up and down of vertical direction are carried out on portal frame
It is dynamic;Sample motion platform 14 is arranged on workbench 100 by sample drive mechanism, below portal frame, is carried out horizontal
The lengthwise movement in direction;Horizontal direction of the target plate motion platform 15 along workbench 100 is located at the side of sample motion platform 14,
It is arranged on by the drive mechanism of target plate 17 on workbench 100, carries out the lengthwise movement of horizontal direction.I.e. with workbench 100
XYZ coordinate system is established, with the place horizontal plane of workbench 100 laterally for X-axis, longitudinal direction is Y-axis, vertical with workbench 100
Vertical plane be Z axis, then microsyringe 1 can carry out back and forth movement, sample motion on portal frame in X-axis and Z-direction
Platform 14 can carry out back and forth movement in the Y-axis direction with target plate motion platform 15, and sample motion platform 14 and target plate motion are flat
The moving line of platform 15 is parallel.
Wherein, sample introduction drive mechanism include be arranged on portal frame horizontal slide rail 7, be slidably arranged in horizontal slide rail 7
Transverse slider 28, drive the cross motor 2 of transverse slider 28, and be arranged on the vertical driving machine of the side of transverse slider 28
Structure.Cross motor 2 drives microsyringe 1 to be moved in horizontal slide rail 7 by drive coordination band 24.In the present embodiment, vertically
Drive mechanism is arranged to two-part driving, then when can reduce the height of vertical direction, while move, can shorten run duration.It is perpendicular
Straight drive mechanism includes being connected to the first order upright slide rail 10 of the side of transverse slider 28, is slidably arranged in first order upright slide rail
First order upright slide block 29, the vertical motor 5 of the first order of driving first order upright slide block 29 on 10, and it is arranged on the first order
Second level upright slide rail 11 on upright slide block 29, the second level upright slide block being slidably arranged on second level upright slide rail 11
30th, the vertical motor 6 in the second level of second level upright slide block 30 is driven;Microsyringe 1 is installed on second level upright slide block 30.
Sample drive mechanism includes first longitudinal direction slide rail 9, drives slide rail to be arranged on the sample motion on first longitudinal direction slide rail 9
The first longitudinal direction motor 4 of platform 14;The drive mechanism of target plate 17 includes second longitudinal direction slide rail 8, driving slide rail is arranged on second longitudinal direction
The second longitudinal direction motor 3 of target plate motion platform 15 on slide rail 8.Wherein, first longitudinal direction slide rail 9 is parallel with second longitudinal direction slide rail 8,
It is vertical with projection of the horizontal slide rail 7 on workbench 100.
The motor that the utility model is related to can be selected linear stepping motor and directly carry out linear drives, can also use stepping
Motor carries out linear drives by shaft coupling and screw mandrel;The slide rail being related to can select single slide rail, double sided slider bar or linear axis backing
Formula.
In order to prevent transverse slider 28, first order upright slide block 29, second level upright slide block 30, sample motion platform 14,
Overexercise occurs for target plate motion platform 15, beyond the movement travel of permission, is then slided vertically in transverse slider 28, the first order respectively
Block 29, second level upright slide block 30, sample motion platform 14, the default end point of travel of target plate motion platform 15 are respectively arranged with
Limit switch, respectively cross spacing switch 19, the vertical limit switch 22 of the first order, the vertical limit switch 23 in the second level, sample
Limit switch 21, target plate limit switch 20.In the present embodiment, it is vertical that limit switch is separately positioned on transverse slider 28, the first order
The presetting origin of sliding block 29, second level upright slide block 30, sample motion platform 14, target plate motion platform 15 be (reset state
Position).When transverse slider 28, first order upright slide block 29, second level upright slide block 30, sample motion platform 14, target plate motion
When platform 15 moves to the position of limit switch restriction, stop motion.When it is implemented, spacing open can be selected according to real needs
The type and model of pass, limit switch can be mechanical limit switch or photo-electric limit switch.
Drier includes drying seal cover board 16, vavuum pump 18, magnetic valve 25, when target plate 17 moves to dry closure
The lower section of plate 16, dry seal cover board 16 and surround sealing space with target plate motion platform 15, target plate 17 is located in sealing space, electromagnetism
Valve 25 is opened, and vavuum pump 18 works, and target plate 17 is dried in vacuo.Vavuum pump 18 is connected with silica gel hose passes through pneumatic element
Target plate 17 is dried with drying seal cover board 16 for fast twist-on head, and magnetic valve 25 is mainly used in keeping the vacuum in drying process
Degree, improves dry efficiency.
The Automated condtrol being related in the utility model, by control main frame 27 by default procedure auto-control, and
It is powered by power supply 26.In particular cases, it is necessary to during the step of limited with default program difference, such as the choosing of sample delivery point
Select, the selection of target spot etc., can be operated by external computer by control main frame 27.Control main frame 27 is receiving computer
Order after, to order parse, according to analysis result complete Automated condtrol.In the utility model, it is related to automation control
The software approach of system can be achieved using prior art, and the content of Automated condtrol program is not as the requires of the utility model
The scope of protection.
In order to regard near operation, also reduce the setting of other accessories, in the utility model, cleaning liquid bath 12, matrix liquid bath 13
It is arranged on workbench 100, control microsyringe 1 moves to cleaning liquid bath 12, is cleaned automatically;Microsyringe 1
Matrix liquid bath 13 is moved to, point takes matrix liquid.
As shown in figure 5, the course of work of the present utility model is as follows:
On full-automatic point sample instrument described in the utility model after electricity, once reset operation (in horizontal slide rail 7, first
Level upright slide rail 10, second level upright slide rail 11, first longitudinal direction slide rail 9, second longitudinal direction slide rail 8 direction on find it is presetting
Origin, clean the syringe needle of a microsyringe 1).
After resetting operation, work order to be received is waited.
After starting work according to work order, the point on sample motion platform 14 of microsyringe 1 takes what is chosen in order
Sample spot, and corresponding target spot carries out printing operation on target plate 17.A selected sample spot is taken at every to take, it is necessary to put
Before next selected sample spot, the syringe needle of microsyringe 1 must clean once in cleaning liquid bath 12.
After all selected sample spots have been put, on the direction of horizontal slide rail 7, microsyringe 1 returns to default
Fixed origin position, and vavuum pump 18 and magnetic valve 25 are opened, target plate 17 is sent into dry seal cover board by target plate motion platform 15
Under 16, target plate 17 is dried once.
After having dried, microsyringe 1 takes matrix liquid at 13 points in matrix liquid bath, is carried out a little for each selected target spot
Matrix liquid operates.After the target spot selected for one carries out fixed point matrix liquid operation, next selected target spot is carried out
Before point matrix liquid operation, the syringe needle of microsyringe 1 must clean once in cleaning liquid bath 12.
After fixed point matrix liquid operations are carried out for all selected target spots, target plate motion platform 15 by target plate 17 again
It is sent into and dries under seal cover board 16, dries target plate 17 once.
Target plate 17 has been carried out second after drying, and the utility model is once resetted operation again.
So far, once complete workflow is completed.
Above-described embodiment is intended merely to illustrate the utility model, and is not used as to restriction of the present utility model.As long as
According to the technical essence of the utility model, above-described embodiment is changed, modification etc. will all fall in right of the present utility model
In the range of it is required that.
Claims (10)
1. a kind of full-automatic point sample instrument, it is characterised in that control microsyringe is laterally moved, vertical in the horizontal direction
Direction is moved up and down, and controls the sample motion platform with array sample to be indulged in the horizontal direction below microsyringe
To motion, the target plate motion platform that control top surface is provided with target plate carries out longitudinal fortune in the horizontal direction below microsyringe
It is dynamic;Microsyringe, sample motion platform, target plate motion platform carry out cooperative motion according to control instruction, and microsyringe is certainly
It is dynamic to complete to be cleaned in rinse bath, be sampled in target sample point, carrying out taking matrix liquid, in target in matrix liquid bath
Carry out point sample and point matrix liquid.
2. full-automatic point sample instrument according to claim 1, it is characterised in that set on the moving line of target plate motion platform
Drier is equipped with, after control target plate motion platform is dried in the range of mechanism, target plate is dried.
3. full-automatic point sample instrument according to claim 1 or 2, it is characterised in that workbench is set, on workbench
Portal frame is set, and microsyringe is connected by sample introduction drive mechanism with portal frame, and the horizontal stroke of horizontal direction is carried out on portal frame
To motion and the up and down motion of vertical direction;Sample motion platform is arranged on workbench by sample drive mechanism, is located at
Below portal frame, the lengthwise movement of horizontal direction is carried out;Horizontal direction of the target plate motion platform along workbench is located at sample fortune
The side of moving platform, it is arranged on by target plate drive mechanism on workbench, carries out the lengthwise movement of horizontal direction.
4. full-automatic point sample instrument according to claim 3, it is characterised in that cleaning liquid bath, matrix liquid bath are arranged on work
On platform, control microsyringe moves to cleaning liquid bath, is cleaned automatically;Microsyringe moves to matrix liquid bath, point
Take matrix liquid.
5. full-automatic point sample instrument according to claim 3, it is characterised in that sample introduction drive mechanism includes being arranged on portal frame
On horizontal slide rail, be slidably arranged in horizontal slide rail transverse slider, drive the cross motor of transverse slider, and be arranged on
The upright driving mechanism of transverse slider side.
6. full-automatic point sample instrument according to claim 5, it is characterised in that upright driving mechanism includes being connected to horizontal cunning
The first order upright slide rail of block side, the first order upright slide block being slidably arranged on first order upright slide rail, the driving first order
The vertical motor of the first order of upright slide block, and be arranged on first order upright slide block second level upright slide rail, slide set
Second level upright slide block, the vertical motor in the second level of driving second level upright slide block on the upright slide rail of the second level;It is micro enter
Sample device is installed on the upright slide block of the second level.
7. full-automatic point sample instrument according to claim 3, it is characterised in that sample drive mechanism is slided including first longitudinal direction
Rail, driving slide rail are arranged on the first longitudinal direction motor of the sample motion platform on first longitudinal direction slide rail;Target plate drive mechanism includes
Second longitudinal direction slide rail, driving slide rail are arranged on the second longitudinal direction motor of the target plate motion platform on second longitudinal direction slide rail.
8. the full-automatic point sample instrument according to any one of claim 5 to 7, it is characterised in that respectively in transverse slider, first
Level upright slide block, second level upright slide block, sample motion platform, the default end point of travel of target plate motion platform are respectively arranged with
Limit switch, when transverse slider, first order upright slide block, second level upright slide block, sample motion platform, target plate motion platform fortune
When moving to the position of limit switch restriction, stop motion.
9. full-automatic point sample instrument according to claim 3, it is characterised in that drier includes drying seal cover board, true
Empty pump, magnetic valve, when target plate is moved to below dry seal cover board, dry seal cover board surrounds sealing sky with target plate motion platform
Between, target plate is located in sealing space, and magnetic valve is opened, and vacuum pump work, target plate is dried in vacuo.
10. full-automatic point sample instrument according to claim 9, it is characterised in that limit switch be mechanical limit switch or
Photo-electric limit switch.
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Cited By (9)
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CN109444449A (en) * | 2018-12-14 | 2019-03-08 | 贵州大学 | A kind of high throughput droplet array microfluid spot sample device |
CN109856417A (en) * | 2019-04-04 | 2019-06-07 | 安图实验仪器(郑州)有限公司 | New type auto micro sample-adding device |
CN110940720A (en) * | 2019-11-25 | 2020-03-31 | 徐州医科大学 | Automatic cellulose acetate film point appearance ware of pressing |
CN112946310A (en) * | 2021-03-11 | 2021-06-11 | 中国汽车技术研究中心有限公司 | Sample applicator |
CN113559947A (en) * | 2021-07-26 | 2021-10-29 | 杭州都林生物科技有限公司 | Automatic sample application instrument |
CN113567598A (en) * | 2021-07-23 | 2021-10-29 | 镇江和瑞环境技术有限公司 | Sample injector with automatic positioning, collecting and cleaning functions and use method thereof |
CN113567536A (en) * | 2021-07-12 | 2021-10-29 | 宁波海关技术中心 | Sample application device for detecting strontium and lead in thermal ionization isotope mass spectrum |
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Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109444449A (en) * | 2018-12-14 | 2019-03-08 | 贵州大学 | A kind of high throughput droplet array microfluid spot sample device |
CN109856417A (en) * | 2019-04-04 | 2019-06-07 | 安图实验仪器(郑州)有限公司 | New type auto micro sample-adding device |
CN110940720A (en) * | 2019-11-25 | 2020-03-31 | 徐州医科大学 | Automatic cellulose acetate film point appearance ware of pressing |
CN112946310A (en) * | 2021-03-11 | 2021-06-11 | 中国汽车技术研究中心有限公司 | Sample applicator |
CN113567536A (en) * | 2021-07-12 | 2021-10-29 | 宁波海关技术中心 | Sample application device for detecting strontium and lead in thermal ionization isotope mass spectrum |
CN113567598A (en) * | 2021-07-23 | 2021-10-29 | 镇江和瑞环境技术有限公司 | Sample injector with automatic positioning, collecting and cleaning functions and use method thereof |
CN113567598B (en) * | 2021-07-23 | 2023-11-28 | 镇江和瑞环境技术有限公司 | Sampler with automatic positioning, collecting and cleaning functions and use method thereof |
CN113559947A (en) * | 2021-07-26 | 2021-10-29 | 杭州都林生物科技有限公司 | Automatic sample application instrument |
CN113559947B (en) * | 2021-07-26 | 2022-05-06 | 杭州都林生物科技有限公司 | Automatic sample application instrument |
WO2023221211A1 (en) * | 2022-05-19 | 2023-11-23 | 厦门元谱生物科技有限公司 | Direct coating device and microrganism direct coating microarrayer applying same |
CN116879483A (en) * | 2023-05-06 | 2023-10-13 | 合肥机数量子科技有限公司 | Automatic quantitative sample application device and automatic sample application method for thin layer chromatography |
CN116879483B (en) * | 2023-05-06 | 2024-01-26 | 合肥机数量子科技有限公司 | Automatic quantitative sample application device and automatic sample application method for thin layer chromatography |
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