CN108459133A - Thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting method - Google Patents
Thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting method Download PDFInfo
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Abstract
The invention discloses thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting methods, including chromatographic sheet box, expansion and colour developing liquid phase groove box, the rotating driving device of mobile chromatographic sheet, infusion pump solvent injection device, infusion pump samples sample spot sample device, dry and image capture apparatus and automatic testing method.The present invention can automatically control sampling in thin-layer chromatographic analysis, point sample, expansion, colour developing, scanning, and real-time online carries out the qualitative or quantitative analysis of sample.Energy real-time tracking sample composition of the invention changes with time and chemical reaction course, saves manpower, improves efficiency, reduces human error.
Description
Technical field
The present invention relates to the chemical-biological sample separate analytical technique of inorganic, organic compound or large biological molecule etc. necks
Domain, and in particular to thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting method.
Background technology
At the beginning of red, orange, green, blue, yellow (ROGBY) (Chromatography) originates in eighties of last century, dosage is generally several to several hectogammas, is
A kind of more practical, effective microseparation analysis method.Most common liquid chromatography has thin-layered chromatography and column chromatography.It is former
Reason is the difference to the adsorption capacity of various sample compositions according to adsorbent of the same race, so that mobile phase (solvent) is flowed through solid phase and (inhales
Attached dose) during, detach the experimental technique of various composition.Common adsorbent has silica gel, aluminium oxide etc..Mobile phase solvent one
As have the various organic solvents such as water, methanol, ethyl alcohol, dichloromethane, ethyl acetate or many of solvent by a certain percentage
Mixed solution.Column chromatography is to detach sample with solvent washing in the pillar for insert absorbent particles glass or other materials
Method.Can with the efficient liquid phase of high pressure washing chromatographic column (High Performance Liquid Chromatography,
HPLC) chromatographic to develop the resolution ratio for improving sample composition, so that column layer method is become most common chemical or biological sample
Method for separating and analyzing.
Thin-layered chromatography is that adsorbent is applied on chromatographic sheet, then adds sample on the sorbent, and solvent is logical
Cross that capillary phenomenon is unfolded on chromatographic sheet and sample composition is detached on chromatographic sheet.High performance thin layer chromatography method
The development of (High Performance Thin Layer Chromatography, HPTLC) can be used as in chemical analysis
Play increasingly consequence.The combination of thin-layered chromatography and other spectroscopic methodologies, the spectrodensitometry of digital camera, biology are anti-
It should shine, the exploitation of nano adsorption layer becomes better and better to the separating effect and accuracy of detection of sample.In recent years to being applied in developing tank
Add external add-in power such as electromagnetic field, high pressure etc. and generate electrophoresis thin-layer chromatography (Pressurized Plannar
Electrochromatography PPEC), high pressure thin-layer chromatography (Overpressured Layer Chromatography
The methods of) OPLC the detection resolution of sample is also improved.(with reference to Colin F.Poole, Instrumental Thin-
Layer Chromatography.Elsevier 2015)
Although in resolution ratio, thin-layered chromatography not as good as high performance liquid chromatography, thin-layered chromatography can be thin at one piece
The multiple samples of parallel determination on layer chromatography plate, analyze speed is fast, easy to operate simple, and can intuitively use image recording point
Analysis.In medicine, the fields such as pharmacy, biochemistry, health, environment, food, chemical industry are widely used.Thin-layer chromatography is frequently used for medium-height grass
Quality management and the native compound of medicine production and identification, discriminating, determination of foreign matter or the assay of chemicals.In drug
In research and development, thin layer chromatography is used to measure quantitative structure activity relationship (the Quantitative Structure of drug development
Activity Relationship, QSAR) the physical-chemical parameters (logP).In addition, thin-layer chromatography bioautography method
(TLC-Bioautography) it is applied to antibacterium, antimycotic drug screening.(with reference to K.Ciura et al, Journal
of Chromatography A,1520(2017)9-22.)
In chemosynthesis reaction detection, initial compounds or generation of the thin-layered chromatography to chemical reaction are typically utilized
Compound is detected with the changes of contents in reaction time, to learn the process of chemosynthesis reaction.
The specific step of thin layer chromatography be sampling, point sample, expansion, drying volatilize point sample or developing solvent, colour developing
The qualitative analysis or progress UV scanning observed under agent colour developing, ultraviolet lamp calculate the quantitative analysis of sample size.
Point sample：Most classical method is sampled with glass capillary, by sample solution point to the chromatographic sheet prepared
On one end, until sample is fixed on the silica gel particle on chromatographic sheet.Solvent is volatilized totally with cool breeze and hot wind, energy
Preferably control arraying quality.
Expansion：Chromatographic sheet one end with sample is put into the solvent of developing tank, sample point will be in solvent circle
On face, solvent can move expansion on chromatographic sheet, due to the adsorption capacity of each ingredient of sample it is different and in thin-layer chromatography
The Rf value (Rf) being unfolded on plate is different, achievees the purpose that separation.Rf value (Retardation factor, Rf) refers to opening up
In open procedure, the ratio between displacement distance and mobile phase solvent displacement distance of sample composition can be expressed with following formula：
Rf=Zx/ (Zf-Z0)
Wherein：
Zx, solvent front is at a distance from origin (at point sample)
Zf, solvent front is at a distance from mobile phase junction
Z0, origin is at a distance from chromatographic sheet and mobile phase junction
When with common chromatographic sheet, Rf values are related with its polarity size.The small Rf values of polarity are larger, and polarity is big
Rf values are smaller.
Colour developing：After chromatographic sheet expansion, chromatographic sheet is put into color developing agent or with color developing agent to chromatographic sheet
Spray, the sample being adsorbed on chromatographic sheet made to react with color developing agent, under room temperature or heating condition sample at
Visible light can be absorbed at expansion and color is presented by dividing.It, can be in chromatographic sheet adsorbent to there is the sample of UV absorption
In fluorescence indicator, such as silica gel plate GF254 is added, generate uniform fluorescence background under the excitation of ultraviolet light, and sample spot
Point is then shown with dead color, and can be photographed to record.
Qualitative analysis：In the case of sample is coloured, the expansion separating resulting of sample can be directly observed with eyes.If
Sample is colourless but has UV absorption, can observe and record separating resulting in the UV lamp using fluorescent thin-layer chromatography plate.In addition may be used
Judged with using color developing agent that the chromatographic sheet after expansion develops the color.
Quantitative analysis：Usually the sample composition after chromatographic sheet expansion is quantified with thin layer chromatography scanning.Thin layer scanning is
With the sample point on visible light or ultraviolet light chromatographic sheet, absorbance (A) travelling with the exhibits on an exhibition tour of thin-layer chromatography spot is measured
The variation for opening distance obtains tlc scanning curve.Quantifying for thin layer chromatography scanning is to be based on Gu Charles Bell Ka-Man Ke
(kubelka-Munk) law.Ancient Charles Bell Ka-Man Ke laws illustrate the diffusion parameter of stationary phase to sample component in spot
The influence of concentration and absorbance relationship.
Ancient Charles Bell Ka-Man Ke (kubelka-Munk) law theoretically can express (reference with following mathematical formulae
Spangenberg B.J.Planner Chromatography 2006；19；332)：
Wherein：
R ∞, the absolute reflectance of infinite thickness adsorption layer
ε, molar absorption coefficient
S, the coefficient of dispersion
C, the spot concentration of thin-layer chromatography
N, mole sample size
A, speck area
D, adsorbent layer thickness
A, absorption coefficient
Sample amounts after being detached to expansion usually require more accurate spectrographic technique.On chromatographic sheet most often at present
Assay method is multiband and diode matrix scanning and the measurement with digital camera to optical density.Quantitative approach, which has, returns
One method, external standard method, additional method and regression curve sizing technique.More commonly used is regression curve method, by standard solution various concentration
With sample solution point on same chromatographic sheet, thin layer scanning is carried out after expansion, obtains the regression curve of standard solution, and
Afterwards sample size is calculated with regression curve.Meanwhile thin-layer chromatography can also be combined with other spectroscopic methodologies such as mass spectrum, infrared and nuclear-magnetism
Resonance carries out more accurate identification and quantification to analysis sample.
In practical operation, thin-layer chromatographic analysis method detection sample is required for through point sample, and solvent expansion separation has
Chemical colour reaction step is needed, it is visual finally by human body eye, or scanned using spectrometer qualitative or quantitative to chemistry and raw
Object sample composition is analyzed.Each step will laboratory technician according to experiment flow step, step by step take action on one's own to operate.
First, on chromatographic sheet point sample, i.e., by sample solution with capillary glass tube drop on chromatographic sheet, wait point samples complete
Afterwards, chromatographic sheet is placed into developing tank and carries out solvent expansion.In this course, sample composition can be because of ingredient to chromatography
The adsorption capacity difference of chromatographic sheet adsorbent is detached.To after expansion as a result, if sample is colourless, need to utilize
Chemochromic reagent develops the color, i.e., taking-up after sample chromatographic sheet being put into developing solution, heating colour developing on hot plate.If
Sample composition has UV absorption, can save chemical colour reaction step, and irradiates lower observation with ultraviolet light, is remembered with hand or instrument
Record separating resulting.
What thin-layer chromatographic analysis operation at present substantially carried out in open environment.Outer bound pair by separation sample effects very
, the influence of solvent vapour, the influence and exhibition of temperature away from influence etc..Thin-layer chromatographic analysis
Overall process be offline many more manipulations, operating process and skill can influence the quality of chromatography.Thin-layer chromatographic analysis usually requires reality
The person of testing waits under lab, goes to complete these steps according to the experiment progress time, can consume laboratory technician's a large amount of time.Together
Sample, when detecting chemical reaction course, full laboratory technician manual operations thin-layer chromatographic analysis, and need with chemical reaction into
Journey is in real time detected chemical reactant and product, and the thin of multiple chemical reactions cannot be carried out at the same time by limiting laboratory technician
Layer chromatography detection operation, in addition, the thin-layer chromatography detection of Qualitative thin-layer's chromatography especially chemosynthesis reaction, only with master
Record tlc analysis is seen as a result, without original objective record, quality management and the standardization of data cannot be preferably carried out and answer
With.
Although each step of thin-layered chromatography is by instrumentation, such as point sample instrument, developing instrument, multiple developing instrument, atomization colour developing
Instrument, UV scanning and integrator etc. have certain improvement to nonstandard operation and efficiency, still, in actual analysis operating process, this
A little instruments are typically the accurate quantitative analysis for handling and carrying out for the chromatographic sheet of a step, and each step will be from
Line operation is completed.Point sample on chromatographic sheet, solvent expansion, dry, chemical colour reaction or UV scanning will be manually by thin layers
Chromatosheet is put into the instrument of each step, and operations is waited for complete, and whole process will be accompanied manually, need to use up operator
The extensive work time.Moreover, each step instrument is all typically used for handling larger lamellae (such as 10x20 centimetres or 20x20 lis
Rice) chromatography or more expensive or bulky and be all off-line operation, limit these instruments for thin-layer chromatography
Analyze the purposes for the simple qualitative comparison being most good at.
It is most efficient method by the on-line analysis detection of each operating procedure full-automation.Due to efficient liquid phase (HPLC)
The chromatographic column of chromatography can be repeatedly for sample analysis and without replacing, the sampling in operation, divides in sample introduction to chromatographic column
It is easier full-automation from detection, the in-line analyzer of efficient liquid phase (HPLC) is begun to early in the eighties in last century
Device.Compared with the efficient liquid phase (HPLC) of column layer chromatography, the lamellae of thin-layered chromatography can typically be only used to one after point sample
The sample of secondary sample analysis, opposed polarity needs different developing solvents, therefore thin-layer chromatography detection and analysis need to be replaced frequently
Chromatographic sheet and developing solvent, the automation of whole analysis operations is opposite to be difficult.With the on-line analysis of efficient liquid phase (HPLC)
It has been that the situation of normality is compared, thin-layered chromatography (TLC) lacks effective real-time on-line detecting method.
Moreover, it is noted that as thin-layered chromatography, many analysis methods are also that a stratification is coated on thin plate
Learn substance and detach purpose in order to which with this layer of chemical substance physically or chemically effect arrival occurs for sample, then to after separation at
Divide and carries out qualitative spectrometric or quantitative analysis.It is main to have the electrophoresis analytical method etc. for being usually used in analyzing protein and DNA.These points
Analysis method operating process having the same, i.e. sampling, point sample, dyeing, image scanning, there is also off-line operation etc. is identical
The problem of.
Invention content
To be solved by this invention is the inefficient of existing thin layer chromatography, and nonstandard artificial off-line operation is more, weight
Existing property difference and quality problem difficult to control, and it is an object of the present invention to provide thin-layer chromatographic analysis automatic intelligent detecting instrument and its exist in real time
Line detecting method, by sampling, point sample, expansion, colour developing, the drying in current chromatographic sheet analytic approach, scanning or image capture
Multiple steps or more instruments are recorded and quantitatively calculated, completed with the automatic detection instrument of the present invention and realize reality
When online thin-layer chromatography determination method.
The automation thin-layer chromatography detection device that the real-time on-line detecting method of the present invention is utilized includes rack, and storage is thin
The chromatographic sheet box of layer chromatography plate, the liquid phase groove box of sample expansion support the sample application platform of chromatographic sheet, mobile thin layer
The rotating driving device of chromatosheet, to the sample spot sample device that point sample position and point sample amount are accurately controlled, and to thin layer
Chromatosheet carries out the equipment and automatic control system of image capture and analysis.
What the solvent expansion slot device of the thin-layer chromatography detection intelligence instrument of the present invention usually carried out is longitudinally perpendicular thin layer
Development of chromatogram mode, the liquid phase groove as expansion are that the rectangular channel being open by a side is constituted, multiple liquid phase groove longitudinal stack structures
At liquid phase groove box.The bottom of liquid phase groove is connect with infusion pump, and infusion pump provides the injection and extraction of developing solvent.Infusion pump includes
But it is not limited to vacuum pump, peristaltic pump, reciprocating pump etc..
According to the specific analytical method of thin-layer chromatography, liquid phase groove, which can be installed, applies electric field, electromagnetism, high-pressure installation to carry out institute
The chromatography needed required device such as in electrophoresis tlc analysis (PPEC), the methods of high pressure tlc analysis (OPLC).
The liquid in-out mouth of infusion pump can be assembled in the appropriate location of liquid phase cell wall, accurately control liquid inlet volume, carry out gradient, expand horizontally
And 2D expansion (2dimensional TLC development).
The sample spot sample device of the present invention can be fixed in closed rack, by infusion pump, horizontal slide unit, longitudinal slide unit
It is constituted with sampling head.Horizontal slide unit and longitudinal slide unit are used for positioning the position of sampling head, and sampling head is connect with infusion pump can be more
It accurately controls point sample amount and improves point sample precision.In addition, the liquid feeding end of infusion pump connects sample solution, it can be molten with timing extraction sample
Liquid carries out point sample.Sampling infusion pump can be combined with crossover valve, while be carried out to the sample solution in multiple containers parallel real-time
On-line period is analyzed.For example, the infusion pump of sampler can be connect with multiple chemical reaction instruments and real-time online detection it is more
The content of a chemical reactant or product and principal reaction process.
There is the image capture analytical equipment of the present invention chromatographic sheet after sample is unfolded to carry out setting for Image Acquisition
Standby including but not limited to ultraviolet, infrared scanner or industrial camera, ultraviolet lamp, fluorescent lamp, heating or blowing-dryer.Image is taken the photograph
Device is taken to be typically mounted on the top of chromatographic sheet sample surface position, after solvent expansion, by UV scanning, ultraviolet irradiation
The operations such as take pictures or take pictures after developing the color carry out image capture and record.
The present invention provides one simply and effectively automation rotating driving device by chromatographic sheet from chromatographic sheet
Sample application platform is skidded off in box and carries out point sample, is then slided into, is skidded off liquid phase groove, can realize and be taken out from chromatographic sheet box one by one
One or more chromatographic sheets carry out chromatography operation.Both ends in this rotating driving device possess chromatographic sheet
Box and liquid phase groove box, rotating driving device center have support and mobile chromatographic sheet sample application platform channel.Adjust thin layer color
The rotation direction for composing the position and rotating driving device of plate box and liquid phase groove box and sample application platform, can move chromatographic sheet
Point sample is carried out above sample application platform and is moved, developing tank is moved into while being injected developping solution progress solvent expansion and then by thin-layer chromatography
Plate is moved out to the operation of multiple steps such as the top progress image capture of plate box, liquid phase groove box or sample application platform.It is all these thin
Layer chromatography detection and analysis operation is controlled by automation control process makes it possible on-line analysis.
The automatic control system of the present invention may include control software and/or the off line PLC control system of PC computer sums to hold
Row control machinery movement instruction and sampling point sample operational order.PC computers have Image Acquisition and quantitative analysis software simultaneously.It is fixed
Regression curve quantitative approach can be utilized by measuring analysis software, acquired the regression curve of standard sample and calculated regression curve formula
Carry out the content of Real_time quantitative detection unknown sample.
The present invention can combine multiple chromatographic sheet boxes, liquid phase groove box and the rotating driving device invented, and can realize
Analysis hundreds of sample within the One step development period realizes such as repeatedly expansion of high-throughput diversified expansion mode, high
Press-stretched is opened, electromagnetism expansion etc. and improve chromatography quality and efficiency.
The present invention can be used for the high throughput and reality in the fields such as medicine, pharmacy, biochemistry, health, environment, food, chemical industry
When online thin-layer chromatographic analysis.For example, detecting instrument of the present invention can be used for identifying the real-time of sample in Chinese herbal medicine production process
Online component content detects to carry out quality management, can be led to the progress of the multiple chemical reactions of the automatic tracing detection of real-time online
The quantitative detection of object is crossed to reactant or produced, the reaction terminating time is identified, to automatically control chemical synthetic reaction.The present invention
Detecting instrument, which saves most of manual operation, can be used for dangerous work environment, such as have severe toxicity, strength irritant gas, reflection
Property substance etc. the detection of real-time online sample composition.
The present invention can be used for preparation of lamina chromatography.Since every piece of chromatographic sheet is all from the recycling of rotating driving device
Channel is recycled, and by recycling adsorbent on chromatographic sheet after acquiring expansion, separation sample composition is obtained with solvent extraction.
In addition to being used for thin-layer chromatographic analysis method, real-time detection method of the invention can be used for using thin plate as sample
The analysis method of product carrier, such as electrophoresis analytical method and cell dyeing analysis.Utilize plate box, liquid phase groove box and the rotation of the present invention
These analysis methods can be become the automation inspection of real-time online by rotary driving device and corresponding image capture analytical equipment
Survey analysis method.
The present invention is realized by following technical operation schemes：
Thin-layer chromatographic analysis automatic intelligent detecting instrument real-time on-line detecting method, the detecting instrument used have storage
The chromatographic sheet box of polylith chromatographic sheet is deposited, the development system containing liquid phase groove box supports the point sample of chromatographic sheet flat
Chromatographic sheet is moved out to sample application platform from chromatographic sheet plate box and chromatographic sheet is moved into, removes liquid phase by platform
The rotating driving device of slot box, sample spot sample device and image capture and analytical equipment can be taken automatically by above-mentioned apparatus combination
Sample, the detection method for carrying out the operating procedure of thin-layer chromatographic analysis and realizing real-time online, the detection method include：
Step 1, equipped with chromatographic sheet chromatographic sheet box and after liquid phase groove box is put into rotating driving device, adjust
The relative altitude of chromatographic sheet box and liquid phase groove box and sample application platform makes chromatographic sheet incline by rotating rotating driving device
Tiltedly, chromatographic sheet is slided out on sample application platform from chromatographic sheet box and is carried out by sample spot sample device under the effect of gravity
Then point sample utilizes same principle to slide into chromatographic sheet in the liquid phase groove of liquid phase groove box；It is thin in the rotating driving device
Layer chromatography plate box and liquid phase groove box are located at sample application platform both ends, and opening corresponds to, can be by thin layer by rotating rotating driving device
Chromatosheet is moved back and forth by sample application platform between chromatographic sheet box and liquid phase groove box；
Step 2 injects the thin layer color that developing solvent carries out various functions into one or more liquid phase grooves of liquid phase groove box
Spectral expansion after expansion is disposed, extracts the developing solvent in liquid phase groove out；The bottom of the liquid phase groove connect with infusion pump and into
The colour developing that color developing agent carries out chromatographic sheet can be injected or be sprayed to the injection or extraction of row developping solution, the liquid phase groove；
Step 3 makes chromatographic sheet skid off liquid phase groove using rotating driving device, into sample application platform, chromatographic sheet
Any place of box top platform, liquid phase groove box top platform carries out image capture analysis, and described image capturing apparatus includes but unlimited
In ultraviolet, infrared scanner, industrial camera, ultraviolet irradiation, drying equipment；
Step 4 is passed through the chromatographic sheet after detection and analysis in chromatographic sheet box using rotating driving device
Recovery approach skids off the recovery bin to lower section and preparation is similarly detected operation by automatic process to next piece of new chromatographic sheet；
The thin-layer chromatography detection point of real-time online is realized by the operational order of automatic control system execution step 1 to 4
Analysis.
Preferred embodiment, the method for carrying out point sample to the chromatographic sheet above sample application platform in the step 1 are：Start sample
The horizontal slide unit of product spot sample device is moved horizontally to longitudinal slide unit the top for the chromatographic sheet for waiting for point sample, and longitudinal slide unit is point
Sample head is moved down into the setpoint distance for the chromatographic sheet for waiting for point sample, and then the liquid outlet of sampling head treats the thin layer of point sample
Chromatosheet carries out point sample, and after the completion of point sample, sample spot sample device resets to home position.
The inlet of preferred embodiment, the sampling head of the sample spot sample device is connect with infusion pump, the sampling of infusion pump into
Liquid mouth is connect with chemistry or biochemical reaction container, automatic time sampling, by sample solution by sampling head in thin-layer chromatography
Point sample is carried out on plate.
The thin-layer chromatographic analysis automatic intelligent detecting instrument of the present invention, including chromatographic sheet box and liquid phase groove box are thin
Sample application platform is equipped between layer chromatography plate box and liquid phase groove box, the opening of chromatographic sheet box and liquid phase groove box is flat towards point sample
Platform, the chromatographic sheet box and liquid phase groove box are connected with the lifting sliding table for adjusting itself and sample application platform relative altitude, thin layer color
Plate box, liquid phase groove box and sample application platform are composed by the synchronouss driving rotation of rotating driving device, in conjunction with gravity realization thin layer color
Transmission of the plate between chromatographic sheet box, liquid phase groove box and sample application platform is composed, the liquid phase groove box is also associated with infusion pump.
Preferred embodiment, the chromatographic sheet box include the holding mechanism that multiple chromatographic sheets can be housed, the appearance
Receiving the top of mechanism is equipped with the recovery approach of chromatographic sheet, and the top of the chromatographic sheet box, which is equipped with, places or transfer thin layer
The chromatographic sheet box top platform of chromatosheet.
The holding mechanism of preferred embodiment, the chromatographic sheet box is that multiple first U-boards and multiple first partitions are alternate
It is formed by stacking, the opening of the multiple first U-board stores chromatographic sheet towards identical in the gap of U-shaped board.
Preferred embodiment, the liquid phase groove box is by multiple second U-shaped boards and multiple second partitions are alternate is formed by stacking, described
The opening of multiple second U-shaped boards is towards identical, and the liquid phase groove box is used to that the solvent of chromatographic sheet to be unfolded, the liquid phase groove
The liquid phase groove box top platform placed or shift chromatographic sheet is equipped at the top of box, the liquid phase trench bottom in the liquid phase groove box is set
There are the manhole appendix of circulation developing solvent, the liquid phase groove of the liquid phase groove box to can be used for developing tank or colour developing slot.
Preferred embodiment, the rotating driving device include frame, and the frame is for fixing and supporting sample application platform, thin layer
Chromatosheet box, liquid phase groove box and lifting sliding table, the left and right ends of the frame, which are fixed, is coated with bearing rod, one end of bearing rod with
The output axis connection of motor, it includes that the first lifting is slided that motor drives the forward and reverse rotation of frame, the lifting sliding table by bearing rod
Platform and the second lifting sliding table, the first lifting sliding table drive the movement of chromatographic sheet box, the second lifting sliding table that liquid phase groove box is driven to move
It is dynamic.
Preferred embodiment, the detecting instrument further include the sample spot sample device being located above the rotating driving device, institute
It states sample spot sample device and point sample operation is carried out to chromatographic sheet, the sample spot sample device includes horizontal slide unit and is located at laterally
Longitudinal slide unit on slide unit, the longitudinal slide unit are equipped with sampling head, and the liquid outlet of the sampling head is located at described in point sample
The surface of sample application platform.
Preferred embodiment, the detecting instrument further include rack, and rotating driving device and sample spot sample device are mounted on and can seal
In the rack closed, the top of the rotating driving device or the front and back Image Acquisition being equipped with to chromatographic sheet progress image capture
Equipment is additionally provided with the ultraviolet lamp being irradiated to chromatographic sheet above rack；It is additionally provided with to thin layer in the bottom of rack
The recovery bin that chromatosheet is collected.
Preferred embodiment, is additionally provided with the first infusion pump for providing liquid phase groove developing solvent or color developing agent in rack, and described the
The inlet of one infusion pump is connect by pipeline with the container of developing solvent or developer, and liquid outlet passes through pipeline and liquid phase groove
Manhole appendix connects；It is additionally provided with the second infusion pump of sampling in rack, the inlet of the second infusion pump passes through pipeline and sample
Container connects, and liquid outlet is connect by pipeline with sampling head.
Preferred embodiment, is additionally provided with air-heater in the pedestal, and the air outlet of the air-heater is to sample application platform, thin-layer chromatography
Plate box top platform and the air-supply of liquid phase groove box top platform, sample application platform, chromatographic sheet box top platform and liquid phase groove box top
It is also equipped with heating plate on the platform of face, heating colour developing is carried out to chromatographic sheet.
Preferred embodiment, the detecting instrument further include automatic control system, and the automatic control system can network intercommunication,
User is controlled or is monitored to detecting instrument by PC computers, work station, intelligent mobile terminal.
Compared with prior art, the present invention having the following advantages and advantages：
1, the present invention is automatically performed the taking during chromatography to chromatographic sheet with a series of automation procedure
Sample, point sample, solvent expansion, colour developing, UV scanning image capture, the multinomial step of data analysis improve work efficiency.
2, the present invention provides a simple and effective rotating driving device and method by chromatographic sheet from thin-layer chromatography
Sample application platform is moved in plate box, the thin layer color that then chromatographic sheet is moved into, liquid phase groove box is removed and realizes real-time online
Spectral analysis method can allow laboratory technician to be detached from the off-line operation of specific tlc analysis, save the working time.
3, the present invention automatic set point sample amount and can be accurately positioned point sample position, reduce artificial error, reduce detection people
The skill set requirements of member.
4, instrument of the invention can control temperature, humidity etc. and automation procedure by sample analysis in the environment of closing
Operation experiments standardize, and increase reproducibility, reduce due to artificial caused error.
5, the combination of chromatographic sheet box of the invention, liquid phase groove box and rotating driving device can realize high-throughput expansion
Mode can detect a large amount of samples within the same expansion period, at the same can also carry out diversified expansion mode such as repeatedly expansion,
High pressure expansion, electromagnetism expansion etc. and improve chromatography quality.
6, the present invention controls developing solvent injection liquid phase groove by infusion pump, can carry out gradient solvent injection, in addition can also
It accurately controls developing solvent and carries out horizontal thin layer expansion and 2D expansion, improve expansion resolution ratio.
7, the present invention detects the reaction process of multiple synthesis chemical reactions, chromatographic sheet box by sample infusion pump simultaneously
Design with developing tank box can carry out real-time online tracing detection to multiple chemical reaction courses simultaneously.
8, instrument of the present invention can be connect with internet and Internet of Things, real-time Transmission image and data information, pass through PC electricity
Brain or mobile terminal realize the real time monitoring to sample detection.It can realize such as severe toxicity, strong stimulation gas under hazardous environment or put
The sample detection of ray substance.
Description of the drawings
Attached drawing described herein is used for providing further understanding the embodiment of the present invention, constitutes one of the application
Point, do not constitute the restriction to the embodiment of the present invention.In the accompanying drawings：
Fig. 1 is the structural perspective of the detecting instrument of the present invention；
Fig. 2 is the structural front view of the detecting instrument of the present invention；
Fig. 3 is the structure top view of the rotating driving device of the present invention；
Fig. 4 is sectional views of the Fig. 3 along the directions A-A；
Fig. 5 is the structural perspective of the rotating driving device of the present invention；
Fig. 6 is the structural perspective of the sample spot sample device of the present invention；
Fig. 7 is the structural perspective of the chromatographic sheet box of the present invention；
Fig. 8 is the structural perspective of the liquid phase groove box of the present invention；
Fig. 9 is the operation process chart of the detecting instrument of the present invention.
Label and corresponding parts title in attached drawing：
1- rotating driving devices, 2- sample spot sample devices, 3- motors, 4- bearing blocks, 5- racks, 6- recovery bins, 7- are ultraviolet
Lamp, 8- air-heaters, the first infusion pumps of 9a-, the second infusion pumps of 9b-, 10- chromatographic sheets, 11- frames, 12- sample application platforms, 13-
Chromatographic sheet box, 14- liquid phase groove boxes, the first lifting sliding tables of 15-, the second lifting sliding tables of 16-, 17- bearing rods, 21- are laterally sliding
Platform, 22- longitudinal slide units, 23- sampling heads, 131- first partitions, the first U-boards of 132-, 133- chromatographic sheet box top platforms,
134- recovery approaches, 141- second partitions, the second U-boards of 142-, 145- liquid phase groove box top platforms, 146- liquid phase grooves.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiment and attached drawing, to this
Invention is described in further detail, and exemplary embodiment of the invention and its explanation are only used for explaining the present invention, do not make
For limitation of the invention.
Embodiment
Fig. 9 shows the work flow flow chart of the detecting instrument of the present invention, the detection of thin-layer chromatographic analysis automatic intelligent
Instrument real-time on-line detecting method includes the following steps：
It is put into chromatographic sheet 10 in step 1, each U-board 132 in chromatographic sheet box 13, then by thin layer color
Spectrum plate box 13 is fixed in the first lifting sliding table 15 in rotating driving device 1.
Step 2, original state are the chromatographic sheet box top platform 133 and liquid phase groove box 14 of chromatographic sheet box 13
Liquid phase groove box top platform 145 is flushed with the top of sample application platform 12.Start automated procedures operation, 15 band of the first lifting sliding table
Dynamic chromatographic sheet box 13 moves up, until first containing chromatographic sheet 10 U-board in chromatographic sheet box 13
132 open end is completely exposed, so that the chromatographic sheet 10 in U-shaped board 132 can skid off, the second lifting sliding table 16 drives liquid phase
Slot box 14 moves up, until the second partition 141 in liquid phase groove 14 exposes.
Step 3 drives rotating driving device 1 to be rotated towards 14 direction of liquid phase groove box by bearing rod 17, chromatographic sheet 10
It is skidded off from chromatographic sheet box 13 to above sample application platform 12, chromatographic sheet 10 is by the second partition 141 of liquid phase groove box 14
It is blocked on sample application platform 12,1 negative direction of rotating driving device is then rotated to horizontal position.
The sliding block of step 4, mobile example spot sample device horizontal slide unit 21, by the sampling head 23 in longitudinal slide unit 22 from first
Beginning position is moved to the setting position of the top of rotating driving device 1, and the sampling head 23 declined in longitudinal slide unit 22 arrives thin layer color
Compose the setting position of plate 10.Start the second infusion pump 9b samplings, sample is injected in thin layer color by contact or cordless
It composes on plate 10.Sampling head 23 is moved back to initial position after point sample is complete, cleans sampling head 23, repeats aforesaid operations by the 2nd sample
Point on chromatographic sheet 10, until detection in need sample spot it is complete until.
The sample solvent that step 5, volatilize naturally point sample solvent or unlatching air-heater 8 volatilize on chromatographic sheet 10；Second
Lifting sliding table 16, which moves up liquid phase groove box 14, allows the notch of liquid phase groove box 14 to expose.Then it is rotated towards 14 direction of liquid phase groove box
Rotary driving device 1 allows chromatographic sheet 10 to slide into as in the liquid phase groove 146 of developing tank, rotating driving device 1 is rotated to perpendicular
The state of straight and liquid phase groove box 14 notch upward, the second lifting sliding table 16 move down liquid phase groove box 14 so that sample application platform
12 side wall covers the notch of liquid phase groove box 14.
Step 6 starts the first infusion pump 9a being connect with expansion liquid container, and quantitative developing solvent is inputted liquid phase groove
The solvent expansion of sample composition is carried out in 146.After being unfolded, developing solvent is taken out from liquid phase groove 146 with the first infusion pump 9a
Go out.First lifting sliding table 15 moves down chromatographic sheet box 13, the chromatographic sheet box top platform of chromatographic sheet box 13
133 flush with sample application platform 12, and the second lifting sliding table 16 moves up liquid phase groove box 14, the slot of the developing tank of liquid phase groove box 14
Mouth exposes, and reversely rotates rotating driving device 1, the chromatographic sheet 10 in developing tank is allowed to skid off thin layer color by sample application platform
It composes on plate box top platform 133, rotating driving device 1 is then rotated to horizontality, to the chromatographic sheet 10 after expansion
Carry out solvent volatilization.Second lifting sliding table 16 moves down liquid phase groove box 14, the liquid phase groove box top platform of liquid phase groove box 14
145 flush with sample application platform 12, then rotate rotating driving device 1 towards 14 direction of liquid phase groove box, and chromatographic sheet 10 is allowed to slide into
On liquid phase groove box top platform 145, rotating driving device 1 is then rotated to horizontality, be then turned on ultraviolet lamp 7 irradiation and
Start image capture device and carries out UV scanning and image capture.It, will expansion if to carry out color development treatment to colourless Sample
Chromatographic sheet 10 afterwards slides into the liquid phase groove 146 as colour developing, injection or spraying color developing agent according to same process, has handled
After drain color developing agent, chromatographic sheet 10 is slided into chromatographic sheet box top platform 133 or liquid phase groove box top with same process
Heating colour developing is carried out on face platform 145, then or is carried out at the same time the image capture of 10 sample of chromatographic sheet.
Step 7, the first lifting sliding table move up chromatographic sheet box 13 so that the recovery approach of chromatographic sheet box 13
134 is parallel with point sample operation post, rotates rotating driving device 1 towards 13 direction of chromatographic sheet box, by chromatographic sheet 10 from liquid
It is slid into recovery bin 6 by recovery approach 134 on phase slot box top platform 145.
Step 8 repeats step 2 to 7 according to automation procedure, skids off the 2nd piece of chromatographic sheet 10 with identical or different
Liquid phase groove 146 carries out the point sample of identical or different sample, and solvent expansion, solvent volatilization, colour developing and the automation of Image Acquisition are grasped
Make and realizes real-time online and detect.
Preferred embodiment scheme, all or part of apparatus are suitable for other sides for utilizing same thin plate sample analysis
Method includes but not limited to electrophoretic analysis, and cell dyeing method etc. can use this all or part of apparatus structure thus by these
Determination method is implemented as the analysis method of real-time online.
As depicted in figs. 1 and 2, the present invention provides thin-layer chromatographic analysis automatic intelligent detecting instruments, including to thin layer
Chromatosheet 10 carries out mobile rotating driving device 1, please refers to shown in Fig. 3, Fig. 4, Fig. 5, and rotating driving device 1 includes：Frame
11, frame 11 is preferably but not limited to be preferably but not limited to as " Jiong " shape for " Qian " shape, sample application platform 12,11 left and right ends of frame
Medium position fixation is coated with bearing rod 17, and the left and right ends of bearing rod 17 have worn bearing block 4 respectively, and bearing block 4 is fixed on
In rack 5.One end of bearing rod 17 is connect by shaft coupling with the rotor of output shaft axle of motor 3, and motor 3 is fixed in rack 5, electricity
Machine 3 drives 1 forward and reverse rotation of rotating driving device by bearing rod 17.Sample application platform 12 is fixedly mounted in frame 11, point sample is flat
Platform 12 is 11 equipartition of frame at the first operating area, that is, 13 storage area of chromatographic sheet box and the second operating area, that is, liquid
14 storage area of phase slot box, the first operating area and the second operating area are located at the left and right sides of frame 11.The first of frame 11
It is equipped with the first lifting sliding table 15 and chromatographic sheet box 13 in operating area, the second lifting sliding table 16 is equipped in the second operating area
With liquid phase groove box 14, for sample application platform 12, the first lifting sliding table 15 in the first operating area drives thin-layer chromatography
Plate box 13 moves up and down, and chromatographic sheet box 13 is removably connected on the sliding shoe of the first lifting sliding table 15, thin
13 exhausting with chromatographic sheet 10 of layer chromatography plate box, can before detection after or detection in replace, with ensure to sample into
The prolonged lasting detection of row.For sample application platform 12, the sliding of the second lifting sliding table 16 in the second operating area
Block drives liquid phase groove box 14 to move up and down, and is carried out sample with realizing different chromatographic sheets 10 being sent into liquid phase groove box 14
The step of solvent expansion, colour developing.The open end of chromatographic sheet box 13 is with the open end of liquid phase groove box 14 both facing to sample application platform
12, in order to which chromatographic sheet 10 in chromatographic sheet box 13 from skidding off into any liquid phase groove box 14, sample application platform 12
The side wall at both ends is adjacent with the open end of the open end of chromatographic sheet box 13 and liquid phase groove box 14 respectively, for closing thin layer color
The open end for composing the open end and liquid phase groove box 14 of plate box 13, to open 13 open end of chromatographic sheet box and liquid phase groove box 14
Open end only need to be moved upwards up to setting position by the first lifting sliding table 15 and the second lifting sliding table 16.
It please refers to shown in Fig. 7, chromatographic sheet box 13 is that multiple first U-boards 132 and multiple first partitions 131 are alternate folded
Add, particular number can be increased or decreased according to specific works amount, and the opening direction of multiple U-boards 132 is identical, thin layer
The top of chromatosheet box 13 is equipped with the chromatographic sheet box top platform 133 placed or shift chromatographic sheet 10, thin-layer chromatography
The left and right sides of plate box top platform 133 and the opposite side of open end are equipped with coaming plate, prevent chromatographic sheet 10 in thin-layer chromatography
Deviate from plate box top platform 133.Air-heater 8 is installed to chromatographic sheet in the top of chromatographic sheet box top platform 133
10 are dried, and the most upper interlayer of chromatographic sheet box 13 is additionally provided with the recovery approach 134 of chromatographic sheet 10, recovery approach
134 left and right ends are equipped with the coaming plate thicker than 10 thickness of chromatographic sheet, and rear and front end communicates, convenient for the thin layer color after using
Spectrum plate 10 skids off rotating driving device 1 by recovery approach 134, drops to centralized collection in the recovery bin of lower section.
Please refer to shown in Fig. 8, liquid phase groove box 14 be by multiple with the first U-shaped board 132 and 131 alternate be superimposed of first partition and
At, or second U-shaped board 142 thicker than the first U-shaped board 132 and first partition 131 and second partition 141 is alternate is formed by stacking,
The opening direction of liquid phase groove box 14 is identical, sample application platform 12 of the notch towards centre.14 top of liquid phase groove box, which is equipped with, to be placed or turns
The liquid phase groove box top platform 145 of chromatographic sheet 10 is moved, chromatographic sheet 10 can be on liquid phase groove box top platform 145
It taken pictures, recorded a video or is scanned by the image capture device that top is installed.The bottom of liquid phase groove box 14 is equipped with liquid manhole appendix, can
Developing solvent or color developing agent are injected liquid phase groove box 14 or developing solvent or developer from liquid phase by the first infusion pump 9a
It is extracted out in slot box 14.Liquid phase groove 146 as colour developing slot can also be equipped with spray channel on the side wall of liquid phase groove 146.Liquid phase groove
Also it can be equipped with the positive and negative electrode of connection DC power supply in 146, DC electric field can be applied and carry out the thin layer solvent exhibition under electric field
It opens, and accelerates sample composition of the separation on chromatographic sheet 10.
Preferred embodiment scheme, as shown in Fig. 1 Fig. 2 and Fig. 6, thin-layer chromatographic analysis automatic intelligent detecting instrument also wraps
The sample spot sample device 2 positioned at 1 top of rotating driving device is included, sample spot sample device 2 carries out cooperateing with work with rotating driving device 1
Industry, the effect of sample spot sample device 2 are that, to the progress point sample operation of chromatographic sheet 10, sample spot sample device 2 includes horizontal slide unit
21 and the longitudinal slide unit 22 that is fixed in horizontal slide unit 21, sampling head 23 is fixed in longitudinal slide unit 22, and sampling head 23 goes out liquid
Mouth is in point sample positioned at the surface of sample application platform 12.When chromatographic sheet 10 needs to carry out point sample, horizontal slide unit 21 first
Longitudinal slide unit 22 is moved to the surface of sample application platform 12, the upper surface of sample application platform 12 placed the chromatographic sheet for waiting for point sample
10, next, longitudinal slide unit 22 drives sampling head 23 to move down, until liquid outlet and the chromatographic sheet 10 of sampling head 23 connect
Stop when close, can ensure sample on chromatographic sheet 10 when sampling head 23 carries out point sample to chromatographic sheet 10 in this way
Repetition is accurately positioned.Longitudinal slide unit 22 on sample spot sample device 2 in order not to interfere a series of rotations of rotating driving device 1,
In point sample just by horizontal slide unit 21 from waiting for being moved at point sample above rotating driving device 1, according to the position pair set
Chromatographic sheet 10 carries out point sample.
Preferred embodiment scheme, as depicted in figs. 1 and 2, rotating driving device 1 and sample spot sample device 2 are mounted on rack 5
Interior, the top of rotating driving device 1, which is equipped with, is scanned chromatographic sheet 10/shooting/image capture device recorded a video, pedestal
5 top is additionally provided with the ultraviolet lamp 7 being irradiated to 1 top of rotating driving device, and the bottom of rack 5 is additionally provided with to thin-layer chromatography
The recovery bin 6 that plate 10 is collected is additionally provided with the first infusion for providing liquid phase groove box 14 developing solvent or color developing agent in rack 5
9a is pumped, the first infusion pump 9a is preferably but not limited to as peristaltic pump, reciprocating pump, vacuum pump etc..The feed liquor of first infusion pump 9a
Mouth is connect by pipeline with developing solvent or reagent container, and liquid outlet passes through pipeline and the liquid manhole appendix of liquid phase groove 146 connects
It connects, is additionally provided with air-heater 8 in pedestal 5, the air outlet of air-heater 8 passes through thin above to ventilation shaft to rotating driving device 1
Layer chromatography plate box top platform 133 or liquid phase groove box top platform 145 are blown, and the drying to chromatographic sheet 10 is accelerated.
Preferred embodiment scheme, the inlet of the second infusion pump 9b of thin-layer chromatographic analysis automatic intelligent detecting instrument with
Sample container is connected by pipeline, can carry out automatic sampling, printing operation by automated procedures.In synthesis chemical reaction inspection
In survey, multiple reaction vessels can be connected simultaneously, the content for detecting reactant or product change with time and principal reaction into
Journey.
Preferred embodiment scheme, thin-layer chromatographic analysis automatic intelligent detecting instrument have automatic control system, the control
System processed includes but not limited to PC computers, PLC control panels, motion control card, motor, motor driving plate and image analysis and storage
And sample software for calculation；The automatic control system is connect with internet and Internet of Things, user by PC computers, work station,
Intelligent mobile terminal is controlled or is monitored to sample detection.
Above-described specific implementation mode has carried out further the purpose of the present invention, technical solution and advantageous effect
It is described in detail, it should be understood that the foregoing is merely the specific implementation mode of the present invention, is not intended to limit the present invention
Protection domain, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should all include
Within protection scope of the present invention.
Claims (13)
1. thin-layer chromatographic analysis automatic intelligent detecting instrument real-time on-line detecting method, which is characterized in that its detection used
There is instrument the chromatographic sheet box of storage polylith chromatographic sheet, the development system containing liquid phase groove box to support thin-layer chromatography
Chromatographic sheet is moved out to sample application platform from chromatographic sheet plate box and chromatographic sheet is moved by the sample application platform of plate
Enter, remove the rotating driving device of liquid phase groove box, sample spot sample device and image capture and analytical equipment, passes through above-mentioned apparatus group
The detection method for closing energy automatic sampling, carrying out the operating procedure of thin-layer chromatographic analysis and realizing real-time online, the detection method
Including：
Step 1, equipped with chromatographic sheet chromatographic sheet box and after liquid phase groove box is put into rotating driving device, adjust thin layer
The relative altitude of chromatosheet box and liquid phase groove box and sample application platform makes chromatographic sheet tilt by rotating rotating driving device,
Chromatographic sheet is slided out on sample application platform from chromatographic sheet box and is carried out a little by sample spot sample device under the effect of gravity
Then sample utilizes same principle to slide into chromatographic sheet in the liquid phase groove of liquid phase groove box；In the rotating driving device, thin layer
Chromatosheet box and liquid phase groove box are located at sample application platform both ends, and opening corresponds to, can be by thin layer color by rotating rotating driving device
Spectrum plate is moved back and forth by sample application platform between chromatographic sheet box and liquid phase groove box；
Step 2 injects the thin-layer chromatography exhibition that developing solvent carries out various functions into one or more liquid phase grooves of liquid phase groove box
It opens, after expansion is disposed, extracts the developing solvent in liquid phase groove out；The bottom of the liquid phase groove connect with infusion pump and is opened up
The injection or extraction of liquid are opened, the colour developing that color developing agent carries out chromatographic sheet can be injected or be sprayed to the liquid phase groove；
Step 3 makes chromatographic sheet skid off liquid phase groove using rotating driving device, into sample application platform, chromatographic sheet box top
Any place of face platform, liquid phase groove box top platform carries out image capture analysis, and described image capturing apparatus includes but not limited to purple
Outside, infrared scanner, industrial camera, ultraviolet irradiation, drying equipment；
Chromatographic sheet after detection and analysis is passed through the recycling in chromatographic sheet box by step 4 using rotating driving device
Channel skids off the recovery bin to lower section and preparation is similarly detected operation by automatic process to next piece of new chromatographic sheet；
The thin-layer chromatography detection and analysis of real-time online are realized by the operational order of automatic control system execution step 1 to 4.
2. thin-layer chromatographic analysis automatic intelligent detecting instrument real-time on-line detecting method according to claim 1, special
Sign is that the method for carrying out point sample to the chromatographic sheet above sample application platform in the step 1 is：Start sample spot sample device
Horizontal slide unit longitudinal slide unit is moved horizontally to the top of the chromatographic sheet for waiting for point sample, longitudinal slide unit is sampling head to moving down
In the setpoint distance for moving the chromatographic sheet for waiting for point sample, then the liquid outlet of sampling head treats the chromatographic sheet progress of point sample
Point sample, after the completion of point sample, sample spot sample device resets to home position.
3. thin-layer chromatographic analysis automatic intelligent detecting instrument real-time on-line detecting method according to claim 2, special
Sign is that the inlet of the sampling head of the sample spot sample device is connect with infusion pump, sampling inlet and the chemistry of infusion pump
Or the connection of biochemical reaction container, automatic time sampling carry out sample solution a little by sampling head on chromatographic sheet
Sample.
4. thin-layer chromatographic analysis automatic intelligent detecting instrument, which is characterized in that thin including chromatographic sheet box and liquid phase groove box
Sample application platform is equipped between layer chromatography plate box and liquid phase groove box, the opening of chromatographic sheet box and liquid phase groove box is flat towards point sample
Platform, the chromatographic sheet box and liquid phase groove box are connected with the lifting sliding table for adjusting itself and sample application platform relative altitude, thin layer color
Plate box, liquid phase groove box and sample application platform are composed by the synchronouss driving rotation of rotating driving device, in conjunction with gravity realization thin layer color
Transmission of the plate between chromatographic sheet box, liquid phase groove box and sample application platform is composed, the liquid phase groove box is also associated with infusion pump.
5. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 4, which is characterized in that the thin layer color
Spectrum plate box includes the holding mechanism that multiple chromatographic sheets can be housed, and the top of the holding mechanism is equipped with chromatographic sheet
The top of recovery approach, the chromatographic sheet box is equipped with placement or the chromatographic sheet box top of transfer chromatographic sheet is flat
Platform.
6. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 5, which is characterized in that the thin layer color
The holding mechanism of spectrum plate box is multiple first U-boards and multiple first partitions are alternate is formed by stacking, the multiple first U-board
Opening stores chromatographic sheet towards identical in the gap of U-shaped board.
7. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 4, which is characterized in that the liquid phase groove
Box is by multiple second U-shaped boards and multiple second partitions are alternate is formed by stacking, and the opening of the multiple second U-shaped board is towards phase
Together, the liquid phase groove box is used to that the solvent of chromatographic sheet to be unfolded, and placement or transfer thin layer are equipped at the top of the liquid phase groove box
The liquid phase groove box top platform of chromatosheet, the liquid phase trench bottom in the liquid phase groove box are equipped with the manhole appendix of circulation developing solvent,
The liquid phase groove of the liquid phase groove box can be used for developing tank or colour developing slot.
8. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 4, which is characterized in that the rotation is driven
Dynamic device includes frame, and the frame is slided for fixing and supporting sample application platform, chromatographic sheet box, liquid phase groove box and lifting
Platform, the left and right ends of the frame, which are fixed, is coated with bearing rod, and one end of bearing rod and the output axis connection of motor, motor pass through
It includes the first lifting sliding table and the second lifting sliding table, the first lifting that bearing rod, which drives the forward and reverse rotation of frame, the lifting sliding table,
Slide unit drives the movement of chromatographic sheet box, the second lifting sliding table to drive the movement of liquid phase groove box.
9. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 4, which is characterized in that the detector
Device further include be located at the rotating driving device above sample spot sample device, the sample spot sample device to chromatographic sheet into
Row point sample operation, the sample spot sample device include horizontal slide unit and the longitudinal slide unit in horizontal slide unit, and the longitudinal direction is sliding
Platform is equipped with sampling head, and the liquid outlet of the sampling head is in point sample positioned at the surface of the sample application platform.
10. according to claim 4-9 any one of them thin-layer chromatographic analysis automatic intelligent detecting instruments, which is characterized in that
The detecting instrument further includes rack, and rotating driving device and sample spot sample device are mounted in closed rack, the rotation
The top of rotary driving device or the front and back image capture device being equipped with to chromatographic sheet progress image capture, in the top of rack
It is additionally provided with the ultraviolet lamp being irradiated to chromatographic sheet；It is additionally provided in the bottom of rack and is returned to what chromatographic sheet was collected
Receive storehouse.
11. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 10, which is characterized in that in rack also
Equipped with the first infusion pump for providing liquid phase groove developing solvent or color developing agent, the inlet of first infusion pump by pipeline with
The container of developing solvent or developer connects, and liquid outlet is connected by pipeline and the manhole appendix of liquid phase groove；It is additionally provided with and takes in rack
Second infusion pump of sample, the inlet of the second infusion pump are connected by the container of pipeline and sample, liquid outlet by pipeline with
Sampling head connects.
12. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 10, which is characterized in that the pedestal
Inside it is additionally provided with air-heater, the air outlet of the air-heater is to sample application platform, chromatographic sheet box top platform and liquid phase groove box top
Face platform is blown, and heating plate pair is also equipped on sample application platform, chromatographic sheet box top platform and liquid phase groove box top platform
Chromatographic sheet carries out heating colour developing.
13. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 10, which is characterized in that the detection
Instrument further includes automatic control system, and the automatic control system can network intercommunication, and user passes through PC computers, work station, intelligence
Energy mobile terminal is controlled or is monitored to detecting instrument.
Priority Applications (2)
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| CN201810326395.2A CN108459133B (en) | 2018-04-12 | 2018-04-12 | Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof |
| PCT/CN2019/082255 WO2019196902A1 (en) | 2018-04-12 | 2019-04-11 | Automated intelligent detection instrument for thin-layer chromatography and real-time online detection method thereof |
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| CN201810326395.2A CN108459133B (en) | 2018-04-12 | 2018-04-12 | Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof |
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| CN108459133A true CN108459133A (en) | 2018-08-28 |
| CN108459133B CN108459133B (en) | 2024-04-23 |
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| CN108459133B (en) | 2024-04-23 |
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