CN108459133B - Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof - Google Patents
Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof Download PDFInfo
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Abstract
The invention discloses an automatic intelligent detection instrument for thin-layer chromatography analysis and a real-time online detection method thereof. The invention can automatically control the steps of sampling, sample application, spreading, color development, scanning and the like in the thin layer chromatography analysis, and can perform qualitative or quantitative analysis of the sample on line in real time. The invention can track the change of the components of the sample along with the time and the chemical reaction process in real time, saves labor, improves the efficiency and reduces human errors.
Description
Technical Field
The invention relates to the technical field of separation and analysis of chemical biological samples of inorganic and organic compounds or biological macromolecules and the like, in particular to an automatic intelligent detection instrument for thin-layer chromatography analysis and a real-time online detection method thereof.
Background
Chromatographic analysis (Chromatography) was initiated in the beginning of the last century, and is generally used in amounts of several to hundreds of micrograms, a more practical and effective method of micro-separation analysis. The most common liquid chromatography methods are thin layer chromatography and column chromatography. The principle is an experimental technique for separating various components in the process of flowing a mobile phase (solvent) through a solid phase (adsorbent) according to the adsorption capacity of the same adsorbent on various sample components. Typical adsorbents are silica gel, alumina, etc. The mobile phase solvent is generally water, methanol, ethanol, dichloromethane, ethyl acetate and other organic solvents or mixed solution of various solvents according to a certain proportion. Column chromatography is a method of separating a sample by filling adsorbent particles into a column of glass or other material and washing with a solvent. The development of high performance liquid chromatography (High Performance Liquid Chromatography, HPLC) which allows for high pressure washing of the column increases the resolution of the sample components, making column chromatography the most common method of separation analysis of chemical or biological samples.
Thin layer chromatography is to spread an adsorbent on a thin layer chromatography plate, then apply a sample to the adsorbent, spread the solvent on the thin layer chromatography plate by capillary phenomenon and separate the sample components on the thin layer chromatography plate. The development of High Performance THIN LAYER Chromatography (HPTLC) has made it increasingly important to be able to be used as a chemical analysis. The thin layer chromatography is combined with other spectroscopy, optical density measurement of a digital camera, biological reaction luminescence, and development of a nano adsorption layer has better and better separation effect and detection precision on samples. In recent years, methods such as electrophoresis thin layer chromatography (Pressurized Plannar Electrochromatography PPEC) and high-pressure thin layer chromatography (Overpressured Layer Chromatography OPLC) have also improved the detection resolution of samples by applying external additional forces such as electromagnetic fields, high pressure, and the like to the development tank. (cf. Colin F. Poole, instrumental Thin-Layer chromatography. Elsevier 2015)
Although thin layer chromatography is inferior to high performance liquid chromatography in resolution, thin layer chromatography can measure multiple samples in parallel on one thin layer chromatography plate, has high analysis speed, is convenient and simple to operate, and can be intuitively analyzed by image recording. Is widely used in the fields of medicine, pharmacy, biochemistry, sanitation, environment, food, chemical industry and the like. Thin layer chromatography is often used for quality control of herbal medicine production and identification, identification of natural compounds, chemical, impurity inspection or content determination. In drug development, thin layer analysis is used to measure quantitative structure-activity relationships (Quantitative Structure Activity Relationship, QSAR) physicochemical parameters (log p) of drug development. In addition, the thin layer chromatography bioautography method (TLC-Bioautography) is applied to antibacterial and antifungal drug screening. (cf. K. Ciura et al Journal of Chromatography A,1520 (2017) 9-22.)
In the detection of chemical synthesis reaction, the progress of chemical synthesis reaction is generally known by detecting the content change of a starting compound or a produced compound of chemical reaction with the reaction time by thin layer chromatography.
The thin layer chromatography comprises the specific steps of sampling, sample application, spreading, drying and volatilizing sample application or spreading solvent, developing color by a color developing agent, and performing qualitative analysis observed under an ultraviolet lamp or performing quantitative analysis of ultraviolet scanning to calculate the content of the sample.
Spotting: the most classical method is to sample with a capillary glass tube, spot the sample solution to one end of the prepared thin layer chromatography plate, and wait until the sample is immobilized on silica gel particles on the thin layer chromatography plate. The solvent is volatilized cleanly by cool air and hot air, and the sample application quality can be better controlled.
And (3) unfolding: one end of a thin layer chromatographic plate with a sample is placed into a solvent in a spreading groove, the sample spots are arranged on a solvent interface, the solvent can be spread on the thin layer chromatographic plate in a moving way, and the specific shift values (Rf) spread on the thin layer chromatographic plate are different due to the different adsorption forces of various components of the sample, so that the purpose of separation is achieved. The ratio shift value (Retardation factor, rf) refers to the ratio of the moving distance of the sample component to the moving distance of the mobile phase solvent during development, and can be expressed by the following formula:
Rf＝Zx/(Zf-Z0)
Wherein:
zx distance of solvent front from origin (point of application)
Zf, distance at the junction of solvent front and mobile phase
Z0, distance between origin and junction of thin layer chromatography plate and mobile phase
When using conventional thin layer chromatography plates, the Rf value is related to the magnitude of its polarity. The Rf value with small polarity is larger, and the Rf value with large polarity is smaller.
Color development: after the thin layer chromatography plate is unfolded, the thin layer chromatography plate is placed into a color developing agent or sprayed by the color developing agent, so that a sample adsorbed on the thin layer chromatography plate reacts with the color developing agent, and sample components can absorb visible light at the unfolded position to display color under normal temperature or heating conditions. For the sample with ultraviolet absorption, a fluorescent indicator such as a silica gel plate GF254 can be added into the adsorbent of the thin-layer chromatographic plate, a uniform fluorescent background is generated under the excitation of ultraviolet light, and the sample spots are displayed in dark color and can be photographed and recorded.
Qualitative analysis: in the case of a colored sample, the result of the unfolding separation of the sample can be directly observed by eyes. If the sample is colorless but has ultraviolet absorption, the separation can be recorded by observation under an ultraviolet lamp using a fluorescent thin layer chromatography plate. Alternatively, the developed thin layer chromatography plate can be developed with a color developer to determine.
Quantitative analysis: thin layer scanning is commonly used to quantify the sample components after thin layer chromatography plate development. The thin layer scanning is to irradiate the sample spot on the thin layer chromatographic plate with visible light or ultraviolet light, and measure the change of absorbance (A) of the thin layer chromatographic spot along with the development distance to obtain a thin layer chromatographic scanning curve. Quantification by thin layer scanning is based on Gu Baier Kalman-Mank (kubelka-Munk) law. Gu Baier Kalman's law describes the effect of the diffusion parameter of the stationary phase on the concentration of the sample components in the spot versus absorbance.
Gu Baier Kalman's (kubelka-Munk) law can theoretically be expressed in terms of the following mathematical formulas (cf. Spangenberg B. J. Planner Chromatography 2006;19; 332):
Wherein:
r infinity, absolute reflectance of infinitely thick adsorbed layers
Epsilon, molar absorption coefficient
S, dispersion coefficient
Spot concentration by thin layer chromatography
N, molar sample size
Spot area A
D, thickness of adsorption layer
A, absorption coefficient
Quantification of the sample after separation by unfolding generally requires more elaborate spectroscopic methods. The most common measurement methods currently used on thin layer chromatography plates are multiband and diode matrix scanning and optical density measurement with digital cameras. The quantitative method comprises normalization method, external standard method, addition method and regression curve quantitative method. The regression curve method is more commonly used, different concentrations of the standard solution and the sample solution are spotted on the same thin layer chromatographic plate, the thin layer scanning is carried out after the development, the regression curve of the standard solution is obtained, and then the content of the sample is calculated by using the regression curve. Meanwhile, the thin layer chromatography can also be combined with other spectroscopy such as mass spectrometry, infrared and nuclear magnetic resonance to more accurately identify and quantify the analysis sample.
In practical applications, thin layer chromatography methods are used to detect samples by spotting, solvent development and separation, and chemical color development steps are sometimes required, and finally chemical and biological sample components are analyzed qualitatively or quantitatively by human eye light visualization or by spectrometer scanning. Each step is performed by experimenters in a step-by-step and in-person manner according to the experimental flow. First, sample application is performed on a thin layer chromatography plate, i.e., a sample solution is dripped on the thin layer chromatography plate by using a glass capillary tube, and after the sample application is completed, the thin layer chromatography plate is put into a spreading groove again for solvent spreading. In this process, the sample components are separated due to the difference in adsorption force of the components to the chromatographic thin layer chromatography plate adsorbent. For the developed result, if the sample is colorless, the sample needs to be developed by using a chemical developing reagent, namely, the sample thin layer chromatography plate is taken out after being placed into a developing solution, and the sample thin layer chromatography plate is heated on a heating plate for developing. If the sample components have ultraviolet absorption, the chemochromic step can be omitted and the separation can be recorded by hand or by instrument, as observed under irradiation with ultraviolet light.
The current thin layer chromatography operation is essentially performed in an open environment. The external influence on the separated sample is great, such as the influence of relative humidity, the influence of solvent vapor, the influence of temperature, the influence of span and the like. The whole process of thin layer chromatography is an off-line multi-step operation, and the operation process and skill can affect the quality of the chromatography. Thin layer chromatography typically requires a laboratory technician to wait in the laboratory for the time required to complete the steps, which can consume significant amounts of time. In addition, qualitative thin-layer chromatography analysis, particularly thin-layer chromatography detection of chemical synthesis reactions, only subjectively records the thin-layer analysis result, has no original objective record, and cannot better perform quality management and data standardization and application.
Although the individual steps of thin layer chromatography are instrumented, such as spotters, spreaders, multi-spreaders, nebulizers, uv scans, and integrators, to improve non-standard operation and efficiency, these instruments are typically quantitative analyses for the precision processing of thin layer chromatography plates in one step, each step being done off-line during the actual analysis operation. The thin layer chromatography plate is spotted, solvent is spread, dried, chemical color development or ultraviolet scanning is carried out by manually putting the thin layer chromatography plate into the instrument of each step, waiting for each operation to be completed, and the whole process needs to be accompanied manually, thus needing to spend a great deal of working time of operators. Moreover, each of the step instruments is typically used for chromatographic analysis of relatively large thin layer plates (e.g., 10x20 cm or 20x20 cm), or is relatively expensive, or is bulky and off-line, limiting the use of these instruments for the most elegant simple qualitative comparison of thin layer chromatography.
On-line analytical testing, which fully automates the individual operating steps, is the most efficient method. Because the chromatographic column for High Performance Liquid Chromatography (HPLC) can be used for sample analysis for many times without replacement, sampling in operation, and separation and detection in the chromatographic column are easier to fully automatic, an online analysis instrument for High Performance Liquid Chromatography (HPLC) is started in the early 80 th century. In comparison with High Performance Liquid Chromatography (HPLC), thin layer plates of thin layer chromatography are usually used for sample analysis only once after spotting, and samples of different polarities require different developing solvents, so thin layer chromatography detection analysis requires frequent replacement of thin layer chromatography plates and developing solvents, and automation of the entire analysis operation is relatively difficult. Thin Layer Chromatography (TLC) lacks an efficient real-time online detection method compared to the state where High Performance Liquid (HPLC) online analysis is already normal.
In addition, it should be noted that, as with thin layer chromatography, many analytical methods are to apply a layer of chemicals to a thin plate so that the sample and the layer of chemicals physically or chemically react to achieve the purpose of separation, and then perform spectroscopic qualitative or quantitative analysis on the separated components. The main methods include electrophoresis analysis methods which are commonly used for analyzing proteins and DNA. The analysis methods have the same operation flow, namely the steps of sampling, sample application, dyeing, image scanning and the like, and have the same problems of offline operation and the like.
Disclosure of Invention
The invention aims to solve the problems of low efficiency, more nonstandard manual off-line operation, poor reproducibility and difficult quality control of the traditional thin layer chromatography, and provides an automatic intelligent detection instrument for thin layer chromatography and a real-time on-line detection method thereof.
The automatic thin layer chromatography detection device used by the real-time online detection method comprises a rack, a thin layer chromatography plate box for storing the thin layer chromatography plate, a liquid phase tank box for expanding samples, a sample application platform for supporting the thin layer chromatography plate, a rotary driving device for moving the thin layer chromatography plate, a sample application device for accurately controlling the sample application position and the sample application amount, equipment for image capturing and analyzing the thin layer chromatography plate and an automatic control system.
The solvent spreading slot device of the intelligent thin-layer chromatography detection instrument is usually performed in a longitudinal vertical thin-layer chromatography spreading mode, wherein a liquid phase slot for spreading is formed by rectangular slots with one opening, and a plurality of liquid phase slots are vertically overlapped to form a liquid phase slot box. The bottom of the liquid phase tank is connected with an infusion pump, and the infusion pump provides injection and extraction of the developing solvent. Infusion pumps include, but are not limited to, vacuum pumps, peristaltic pumps, reciprocating pumps, and the like.
Depending on the specific analytical method of thin layer chromatography, the liquid phase tank may be equipped with means for applying an electric field, electromagnetic, high voltage to perform the desired chromatographic analysis, such as those required in the methods of electrophoresis thin layer analysis (PPEC), high voltage thin layer analysis (OPLC), etc. The liquid inlet and outlet of the infusion pump can be assembled at a proper position of the wall of the liquid phase tank, the liquid inlet amount is accurately controlled, and gradient, horizontal expansion and 2D expansion (2 dimensional TLC development) are carried out.
The sample application device can be fixed in a closable frame and consists of an infusion pump, a transverse sliding table, a longitudinal sliding table and an application head. The horizontal slipway and the longitudinal slipway are used for positioning the position of the sample application head, and the sample application head is connected with the infusion pump, so that the sample application amount can be controlled more accurately, and the sample application precision is improved. In addition, the liquid inlet end of the infusion pump is connected with the sample solution, and the sample solution can be regularly pumped for sample application. The sampling infusion pump can be combined with the exchange valve, and simultaneously, sample solutions in a plurality of containers are subjected to parallel real-time online sampling analysis. For example, the infusion pump of the sampling device may be connected to a plurality of chemical reaction instruments to detect the contents of a plurality of chemical reactants or products on line in real time to learn the progress of the reaction.
The image capture analysis device of the present invention has a device for image acquisition of a thin layer chromatography plate after sample development including, but not limited to, an ultraviolet, infrared scanner or industrial camera, an ultraviolet lamp, a fluorescent lamp, a heating or a blow dryer. The image capturing device is usually installed above the sample surface of the thin layer chromatography plate, and after the solvent is spread, the image capturing and recording are performed through operations such as ultraviolet scanning, ultraviolet irradiation photographing or photographing after color development.
The invention provides a simple and effective automatic rotary driving device which slides a thin layer chromatography plate out of a thin layer chromatography plate box to a sample platform for sample application, then slides the thin layer chromatography plate into a slide-out phase groove, and can realize that one or more thin layer chromatography plates are taken out of the thin layer chromatography plate box one by one for chromatographic analysis operation. The thin layer chromatography plate box and the liquid phase tank box are arranged at two ends of the rotary driving device, and the center of the rotary driving device is provided with a thin layer chromatography plate sample application platform supporting and moving channel. The thin layer chromatography plate can be moved to the upper surface of the sample application platform for sample application, moved into the spreading groove for solvent spreading, and then moved out to the top of the plate box, the liquid phase tank box or the sample application platform for image capturing and other steps. All of these thin layer chromatography detection analysis operations are controlled by an automated control process to enable real-time on-line analysis.
The automated control system of the present invention may include a PC and control software and/or an off-line PLC control system to execute the control mechanical movement instructions as well as the sample application process instructions. Meanwhile, the PC computer is provided with image acquisition and quantitative analysis software. The quantitative analysis software can collect the regression curve of the standard sample by using a regression curve quantitative method and calculate a regression curve formula to quantitatively detect the content of the unknown sample in real time.
The invention can combine a plurality of thin-layer chromatographic plate boxes, liquid-phase tank boxes and the rotary driving device, can analyze hundreds or thousands of samples in one unfolding period, and can realize high-flux diversified unfolding modes such as multiple unfolding, high-pressure unfolding, electromagnetic unfolding and the like so as to improve chromatographic analysis quality and efficiency.
The invention can be used for high-flux and real-time online thin-layer chromatography analysis in the fields of medicine, pharmacy, biochemistry, sanitation, environment, food, chemical industry and the like. For example, the detection instrument can be used for identifying the real-time on-line component content detection of the samples in the production process of the Chinese herbal medicines for quality management, can automatically track and detect the progress of a plurality of chemical reactions on line in real time, and can identify the reaction termination time through quantitative detection of reactants or products so as to automatically control the chemical synthesis reaction. The detection instrument of the invention omits most manual operations and can be used for detecting components of real-time on-line samples in dangerous working environments, such as extremely toxic, powerful stimulation gas, reflective substances and the like.
The invention can be used for preparative thin layer chromatography. Since each thin layer chromatography plate is recovered from the recovery channel of the rotary drive device, the separated sample components are obtained by collecting and expanding the adsorbent on the recovered thin layer chromatography plate and extracting the adsorbent with a solvent.
In addition to the thin layer chromatography method, the real-time detection method of the present invention can also be used for an analysis method using a thin plate as a sample carrier, such as an electrophoresis analysis method, a cell staining analysis, and the like. The plate box, the liquid phase tank box, the rotary driving device and the corresponding image shooting analysis device can change the analysis methods into real-time on-line automatic detection analysis methods.
The invention is realized by the following technical operation scheme:
An automatic intelligent detection instrument for thin layer chromatography real-time on-line detection method, wherein the detection instrument comprises a thin layer chromatography plate box for storing a plurality of thin layer chromatography plates, an unfolding system for containing a liquid phase tank box, a sample application platform for supporting the thin layer chromatography plates, a rotary driving device for moving the thin layer chromatography plates out of the thin layer chromatography plate box to the sample application platform and moving the thin layer chromatography plates into and out of the liquid phase tank box, a sample application device and an image capturing and analyzing device, and the detection method can be realized in real-time on-line by combining the above devices to automatically sample and carry out the operation steps of thin layer chromatography analysis, and comprises the following steps:
Step1, after a thin layer chromatography plate box and a liquid phase tank box which are provided with the thin layer chromatography plate are placed into a rotary driving device, the relative heights of the thin layer chromatography plate box and the liquid phase tank box as well as a sample application platform are adjusted, the thin layer chromatography plate is inclined by rotating the rotary driving device, the thin layer chromatography plate slides out of the thin layer chromatography plate box to the sample application platform under the action of gravity and is applied with sample application through a sample application device, and then the thin layer chromatography plate slides into a liquid phase tank of the liquid phase tank box by utilizing the same principle; in the rotary driving device, the thin-layer chromatography plate box and the liquid-phase tank box are positioned at two ends of the sample application platform, openings correspond to each other, and the thin-layer chromatography plate can be moved back and forth between the thin-layer chromatography plate box and the liquid-phase tank box through the sample application platform by rotating the rotary driving device;
Step 2, injecting developing solvent into one or more liquid phase tanks of the liquid phase tank box to perform thin layer chromatography development of various functions, and extracting the developing solvent in the liquid phase tank after the development treatment is finished; the bottom of the liquid phase tank is connected with an infusion pump to inject or extract developing solution, and the liquid phase tank can inject or spray a developing agent to develop the thin-layer chromatographic plate;
Step 3, enabling the thin layer chromatographic plate to slide out of the liquid phase groove by using a rotary driving device, and enabling the thin layer chromatographic plate to enter any one of a sample application platform, a thin layer chromatographic plate box top surface platform and a liquid phase groove box top surface platform for image shooting analysis, wherein the image shooting device comprises, but is not limited to, an ultraviolet scanner, an infrared scanner, an industrial camera, ultraviolet irradiation and drying equipment;
Step 4, sliding the thin-layer chromatography plate after detection and analysis to a lower recovery bin through a recovery channel in the thin-layer chromatography plate box by using a rotary driving device, and preparing the same detection operation for the next new thin-layer chromatography plate by an automatic process;
and (3) executing the operation instructions in the steps 1 to 4 by an automatic control system to realize real-time online thin layer chromatography detection analysis.
Preferably, the method for spotting the thin layer chromatography plate on the spotting platform in the step 1 comprises the following steps: starting a transverse sliding table of the sample application device to horizontally move the longitudinal sliding table to the upper side of the thin layer chromatography plate to be applied with sample, downwardly moving the sample application head to the set distance of the thin layer chromatography plate to be applied with sample by the longitudinal sliding table, then applying sample to the thin layer chromatography plate to be applied with the liquid outlet of the sample application head, and resetting the sample application device to the original position after the sample application is completed.
Preferably, a liquid inlet of a sample application head of the sample application device is connected with an infusion pump, a sampling liquid inlet of the infusion pump is connected with a chemical or biochemical reaction container, sampling is automatically performed at regular time, and sample application is performed on sample solution on a thin-layer chromatographic plate through the sample application head.
The invention relates to an automatic intelligent detection instrument for thin-layer chromatography analysis, which comprises a thin-layer chromatography plate box and a liquid-phase tank box, wherein a sample application platform is arranged between the thin-layer chromatography plate box and the liquid-phase tank box, openings of the thin-layer chromatography plate box and the liquid-phase tank box face the sample application platform, the thin-layer chromatography plate box and the liquid-phase tank box are connected with a lifting sliding table for adjusting the relative height of the thin-layer chromatography plate box and the liquid-phase tank box and the sample application platform, the thin-layer chromatography plate box, the liquid-phase tank box and the sample application platform are synchronously driven to rotate through a rotary driving device, and the thin-layer chromatography plate is conveyed among the thin-layer chromatography plate box, the liquid-phase tank box and the sample application platform under the action of gravity.
Preferably, the thin layer chromatography plate box comprises a containing mechanism capable of containing a plurality of thin layer chromatography plates, a recycling channel of the thin layer chromatography plates is arranged at the top of the containing mechanism, and a thin layer chromatography plate box top surface platform for placing or transferring the thin layer chromatography plates is arranged at the top of the thin layer chromatography plate box.
Preferably, the accommodating mechanism of the thin-layer chromatography plate box is formed by alternately superposing a plurality of first U-shaped plates and a plurality of first partition plates, the openings of the plurality of first U-shaped plates face the same direction, and the thin-layer chromatography plates are stored in the gaps of the U-shaped plates.
Preferably, the liquid phase tank box is formed by alternately superposing a plurality of second U-shaped plates and a plurality of second partition plates, the openings of the second U-shaped plates face the same direction, the liquid phase tank box is used for expanding the solvent of the thin layer chromatographic plate, a liquid phase tank box top surface platform for placing or transferring the thin layer chromatographic plate is arranged at the top of the liquid phase tank box, an inlet and outlet hole for circulating expanded solvent is formed at the bottom of a liquid phase tank in the liquid phase tank box, and the liquid phase tank of the liquid phase tank box can be used for expanding grooves or developing grooves.
Preferably, the rotary driving device comprises a frame, the frame is used for fixing and supporting the sample application platform, the thin-layer chromatography plate box, the liquid-phase groove box and the lifting sliding table, a bearing rod is fixedly sleeved at the left end and the right end of the frame, one end of the bearing rod is connected with an output shaft of the motor, the motor drives the frame to rotate forward and backward through the bearing rod, the lifting sliding table comprises a first lifting sliding table and a second lifting sliding table, the first lifting sliding table drives the thin-layer chromatography plate box to move, and the second lifting sliding table drives the liquid-phase groove box to move.
Preferably, the detecting instrument further comprises a sample application device positioned above the rotary driving device, the sample application device applies an application sample to the thin-layer chromatographic plate, the sample application device comprises a transverse sliding table and a longitudinal sliding table positioned on the transverse sliding table, an application sample head is arranged on the longitudinal sliding table, and a liquid outlet of the application sample head is positioned right above the application sample platform during application sample.
Preferably, the detecting instrument further comprises a frame, the rotary driving device and the sample spotting device are arranged in the closable frame, image acquisition equipment for image capturing of the thin layer chromatography plate is arranged above or in front of and behind the rotary driving device, and an ultraviolet lamp for irradiating the thin layer chromatography plate is arranged above the frame; the bottom of the frame is also provided with a recovery bin for collecting the thin-layer chromatographic plates.
The frame is also internally provided with a first infusion pump for providing developing solvent or color developing agent for the liquid phase tank, a liquid inlet of the first infusion pump is connected with a container of the developing solvent or the color developing agent through a pipeline, and a liquid outlet of the first infusion pump is connected with an inlet and outlet hole of the liquid phase tank through a pipeline; the frame is also internally provided with a second infusion pump for sampling, a liquid inlet of the second infusion pump is connected with a container of the sample through a pipeline, and a liquid outlet of the second infusion pump is connected with the sample application head through a pipeline.
Preferably, still be equipped with the air heater in the bed frame, the air outlet of air heater is to sample application platform, thin layer chromatography board box top surface platform and liquid phase groove box top surface platform air supply, and the heating plate is still installed on sample application platform, thin layer chromatography board box top surface platform and the liquid phase groove box top surface platform and is heated the color development to the thin layer chromatography board.
Preferably, the detecting instrument further comprises an automatic control system, the automatic control system can be connected with each other in a networking mode, and a user controls or monitors the detecting instrument through a PC (personal computer), a workstation and an intelligent mobile terminal.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. The invention automatically completes a plurality of steps of sampling, sample application, solvent spreading, color development, ultraviolet scanning image shooting and data analysis of the thin-layer chromatographic plate in the chromatographic analysis process by a series of automatic working procedures, thereby improving the working efficiency.
2. The invention provides a simple and effective rotary driving device and method for moving the thin-layer chromatography plate from the thin-layer chromatography plate box to the spot sample platform, and then moving the thin-layer chromatography plate into and out of the liquid-phase tank box to realize a real-time online thin-layer chromatography analysis method, so that an experimenter can be separated from the specific off-line operation of thin-layer analysis, and the working time is saved.
3. The invention can automatically set the sample application amount and accurately position the sample application position, reduce the artificial error and reduce the skill requirement of the detection personnel.
4. The instrument can control temperature, humidity and the like and an automation procedure in a closed environment to standardize a sample analysis operation experiment, increase reproducibility and reduce errors caused by manpower.
5. The combination of the thin-layer chromatography plate box, the liquid-phase tank box and the rotary driving device can realize a high-flux unfolding mode, can detect a large number of samples in the same unfolding period, and can also carry out diversified unfolding modes such as multiple unfolding, high-pressure unfolding, electromagnetic unfolding and the like so as to improve the chromatography analysis quality.
6. According to the invention, the developing solvent is controlled by the infusion pump to be injected into the liquid phase tank, so that gradient solvent injection can be performed, in addition, the developing solvent can be accurately controlled to perform horizontal thin layer development and 2D development, and the developing resolution is improved.
7. The invention can simultaneously detect the reaction progress of a plurality of synthetic chemical reactions through the sample infusion pump, and the design of the thin-layer chromatographic plate box and the unfolding groove box can simultaneously carry out real-time online tracking detection on the plurality of chemical reaction progress.
8. The instrument can be connected with the Internet and the Internet of things, can transmit image and data information in real time, and can realize real-time monitoring of sample detection through a PC (personal computer) or a mobile terminal. Can realize the sample detection of highly toxic, strongly stimulated gas or radioactive substances in dangerous environments.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings:
FIG. 1 is a perspective view of the structure of the detecting instrument of the present invention;
FIG. 2 is a front view of the structure of the detecting instrument of the present invention;
FIG. 3 is a top plan view of the rotary drive device of the present invention;
FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is a perspective view of the rotary driving device of the present invention;
FIG. 6 is a perspective view of the structure of the sample application device of the present invention;
FIG. 7 is a perspective view of the structure of the thin layer chromatography plate cassette of the present invention;
fig. 8 is a structural perspective view of the liquid-phase tank cartridge of the present invention;
fig. 9 is a flow chart of the operation of the detecting instrument of the present invention.
In the drawings, the reference numerals and corresponding part names:
The device comprises a 1-rotation driving device, a 2-sample spotting device, a 3-motor, a 4-bearing seat, a 5-rack, a 6-recovery bin, a 7-ultraviolet lamp, an 8-hot air blower, a 9 a-first infusion pump, a 9 b-second infusion pump, a 10-thin layer chromatography plate, a 11-frame, a 12-spotting platform, a 13-thin layer chromatography plate box, a 14-liquid phase tank box, a 15-first lifting sliding table, a 16-second lifting sliding table, a 17-bearing rod, a 21-transverse sliding table, a 22-longitudinal sliding table, a 23-spotting head, a 131-first partition plate, a 132-first U-shaped plate, a 133-thin layer chromatography plate box top surface platform, a 134-recovery channel, a 141-second partition plate, a 142-second U-shaped plate, a 145-liquid phase tank box top surface platform and a 146-liquid phase tank.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
Examples
Fig. 9 shows a flow chart of the operation of the detecting instrument of the present invention, and the real-time on-line detecting method of the automated intelligent detecting instrument for thin layer chromatography comprises the following steps:
Step 1, the thin layer chromatography plate 10 is placed in each U-shaped plate 132 in the thin layer chromatography plate box 13, and then the thin layer chromatography plate box 13 is fixed on the first lifting sliding table 15 in the rotary driving device 1.
Step 2, the initial state is that the thin layer chromatography plate cartridge top surface platform 133 of the thin layer chromatography plate cartridge 13 and the liquid phase tank cartridge top surface platform 145 of the liquid phase tank cartridge 14 are flush with the top of the spotting platform 12. The automatic program operation is started, the first lifting sliding table 15 drives the thin layer chromatography plate box 13 to move upwards until the opening end of the first U-shaped plate 132 containing the thin layer chromatography plate 10 in the thin layer chromatography plate box 13 is completely exposed, so that the thin layer chromatography plate 10 in the U-shaped plate 132 can slide out, and the second lifting sliding table 16 drives the liquid phase tank box 14 to move upwards until the second partition plate 141 on the liquid phase tank 14 is exposed.
Step 3, the rotation driving device 1 is driven to rotate towards the liquid phase tank box 14 through the bearing rod 17, the thin layer chromatography plate 10 slides out of the thin layer chromatography plate box 13 onto the sample application platform 12, the thin layer chromatography plate 10 is blocked on the sample application platform 12 by the second partition plate 141 of the liquid phase tank box 14, and then the rotation driving device 1 is rotated to a horizontal position in the opposite direction.
And 4, moving the slide block of the transverse sliding table 21 of the sample spotting device, moving the spotting head 23 on the longitudinal sliding table 22 from an initial position to a set position above the rotary driving device 1, and lowering the spotting head 23 on the longitudinal sliding table 22 to the set position of the thin-layer chromatographic plate 10. The second infusion pump 9b is activated to sample and the sample is sprayed on the thin layer chromatography plate 10 by contact or non-contact means. After spotting, the print head 23 is moved back to the initial position, the print head 23 is cleaned, and the above operation is repeated to spot the 2 nd sample on the thin layer chromatography plate 10 until all samples to be detected are spotted.
Step 5, naturally volatilizing the sample application solvent or starting the air heater 8 to volatilize the sample solvent on the thin layer chromatographic plate 10; the second elevating slide 16 moves the liquid-phase tank 14 upward to expose the notch of the liquid-phase tank 14. Then the rotary driving device 1 is rotated toward the liquid-phase tank 14 to slide the thin-layer chromatography plate 10 into the liquid-phase tank 146 as a spreading slot, the rotary driving device 1 is rotated to a state in which the vertical and liquid-phase tank 14 is upward in the slot opening, and the second elevating slide table 16 moves the liquid-phase tank 14 downward so that the side wall of the spotting platform 12 covers the slot opening of the liquid-phase tank 14.
Step 6, the first infusion pump 9a connected to the developing solution container is started, and a predetermined amount of developing solvent is fed into the liquid phase tank 146 to develop the solvent of the sample component. After the expansion, the expansion solvent is pumped out of the liquid phase tank 146 by the first liquid transfer pump 9 a. The first lifting sliding table 15 moves the thin-layer chromatography plate box 13 downwards, the thin-layer chromatography plate box top surface platform 133 of the thin-layer chromatography plate box 13 is flush with the sample application platform 12, the second lifting sliding table 16 moves the liquid-phase tank box 14 upwards, the notch of the spreading slot of the liquid-phase tank box 14 is exposed, the rotary driving device 1 is rotated reversely, the thin-layer chromatography plate 10 in the spreading slot slides out onto the thin-layer chromatography plate box top surface platform 133 through the sample application platform, then the rotary driving device 1 is rotated to be in a horizontal state, and solvent volatilization is carried out on the spread thin-layer chromatography plate 10. The second lifting sliding table 16 moves the liquid-phase tank 14 downwards, flushes the liquid-phase tank top surface platform 145 of the liquid-phase tank 14 with the sample application platform 12, then rotates the rotary driving device 1 towards the direction of the liquid-phase tank 14, slides the thin-layer chromatography plate 10 onto the liquid-phase tank top surface platform 145, then rotates the rotary driving device 1 to a horizontal state, and then starts the ultraviolet lamp 7 to irradiate and starts the image acquisition equipment to perform ultraviolet scanning and image capturing. If a colorless sample is to be developed, the developed thin-layer chromatography plate 10 is slid into a liquid tank 146 for developing according to the same procedure, a developer is injected or sprayed, the developer is pumped out after the treatment, the thin-layer chromatography plate 10 is slid onto the thin-layer chromatography plate cartridge top surface platform 133 or the liquid tank cartridge top surface platform 145 for heating development by the same procedure, and then or simultaneously image capturing of the sample of the thin-layer chromatography plate 10 is performed.
Step 7, the first lifting sliding table moves the thin-layer chromatography plate box 13 upwards, so that the recovery channel 134 of the thin-layer chromatography plate box 13 is parallel to the sample application table, the rotary driving device 1 rotates towards the direction of the thin-layer chromatography plate box 13, and the thin-layer chromatography plate 10 slides into the recovery bin 6 from the liquid-phase tank box top surface platform 145 through the recovery channel 134.
And 8, repeating the steps 2 to 7 according to an automatic procedure, and sliding out the 2 nd thin layer chromatography plate 10 to sample the same or different samples by using the same or different liquid phase grooves 146, and performing automatic operations of solvent unfolding, solvent volatilization, color development and image acquisition to realize real-time online detection.
In preferred embodiments, all or part of the instrument apparatus is suitable for use in other methods of analysis using the same sheet sample, including but not limited to electrophoresis, cell staining, etc., and all or part of the present instrument structure may be used to implement these detection analysis methods as real-time on-line analysis methods.
As shown in fig. 1 and 2, the present invention provides an automated intelligent detection apparatus for thin layer chromatography, comprising a rotary driving device 1 for moving a thin layer chromatography plate 10, and referring to fig. 3,4 and 5, the rotary driving device 1 comprises: the frame 11, the frame 11 is preferably but not limited to be 凵-shaped, the sample application platform 12 is preferably but not limited to be 冂-shaped, the middle of the left and right ends of the frame 11 is fixedly sleeved with the bearing rods 17, the left and right ends of the bearing rods 17 are respectively sleeved with the bearing seats 4, and the bearing seats 4 are fixed on the frame 5. One end of a bearing rod 17 is connected with a rotor output shaft of the motor 3 through a coupler, the motor 3 is fixed on the frame 5, and the motor 3 drives the rotary driving device 1 to rotate forward and backward through the bearing rod 17. The sample application platform 12 is fixedly arranged in the frame 11, and the sample application platform 12 evenly divides the frame 11 into a first operation area, namely a thin layer chromatography plate box 13 storage area, and a second operation area, namely a liquid phase tank box 14 storage area, wherein the first operation area and the second operation area are positioned on the left side and the right side of the frame 11. The first operation area of the frame 11 is internally provided with a first lifting sliding table 15 and a thin-layer chromatography plate box 13, the second operation area is internally provided with a second lifting sliding table 16 and a liquid-phase groove box 14, and for the sample application platform 12, the first lifting sliding table 15 in the first operation area drives the thin-layer chromatography plate box 13 to move up and down, the thin-layer chromatography plate box 13 is detachably connected to a sliding block of the first lifting sliding table 15, and the thin-layer chromatography plate box 13 can be replaced before and after detection or during detection along with the exhaustion of the thin-layer chromatography plate 10 so as to ensure long-time continuous detection of samples. Compared with the sample application platform 12, the sliding block of the second lifting sliding table 16 in the second working area drives the liquid phase groove box 14 to move up and down so as to realize the steps of feeding different thin layer chromatographic plates 10 into the liquid phase groove box 14 and developing the solvent of the sample. The open end of the thin layer chromatography plate box 13 and the open end of the liquid phase tank box 14 face the sample application platform 12 so that the thin layer chromatography plate 10 slides out of the thin layer chromatography plate box 13 and enters any liquid phase tank box 14, the side walls of the two ends of the sample application platform 12 are adjacent to the open end of the thin layer chromatography plate box 13 and the open end of the liquid phase tank box 14 respectively and are used for closing the open end of the thin layer chromatography plate box 13 and the open end of the liquid phase tank box 14, and if the open end of the thin layer chromatography plate box 13 and the open end of the liquid phase tank box 14 are required to be opened, the thin layer chromatography plate 10 only needs to be moved upwards to a set position through the first lifting sliding table 15 and the second lifting sliding table 16.
Referring to fig. 7, the thin-layer chromatography plate box 13 is formed by overlapping a plurality of first U-shaped plates 132 and a plurality of first partition plates 131 alternately, the specific number can be increased or decreased according to specific workload, the opening directions of the plurality of U-shaped plates 132 are the same, the top of the thin-layer chromatography plate box 13 is provided with a thin-layer chromatography plate box top surface platform 133 for placing or transferring the thin-layer chromatography plate 10, and the left and right sides and the opposite sides of the opening ends of the thin-layer chromatography plate box top surface platform 133 are provided with coamings for preventing the thin-layer chromatography plate 10 from falling out on the thin-layer chromatography plate box top surface platform 133. The air heater 8 is arranged above the platform 133 on the top surface of the thin-layer chromatographic plate box to dry the thin-layer chromatographic plate 10, the uppermost interlayer of the thin-layer chromatographic plate box 13 is also provided with a recovery channel 134 of the thin-layer chromatographic plate 10, the left end and the right end of the recovery channel 134 are provided with coamings thicker than the thickness of the thin-layer chromatographic plate 10, the front end and the rear end are communicated, and the thin-layer chromatographic plate 10 after use can be conveniently slipped out of the rotary driving device 1 through the recovery channel 134 and falls into a recovery bin below to be collected in a concentrated manner.
Referring to fig. 8, the liquid-phase tank 14 is formed by overlapping a plurality of first U-shaped plates 132 and first partition plates 131, or overlapping second U-shaped plates 142 and second partition plates 141 thicker than the first U-shaped plates 132 and the first partition plates 131, the liquid-phase tank 14 has the same opening direction, and the notch faces the middle spotting platform 12. The top of the liquid tank 14 is provided with a liquid tank top surface platform 145 for placing or transferring the thin layer chromatography plate 10, and the thin layer chromatography plate 10 can be photographed, recorded or scanned by an image acquisition device arranged above the liquid tank top surface platform 145. The bottom of the liquid tank 14 is provided with a liquid inlet and outlet hole, and the developing solvent or the developer can be injected into the liquid tank 14 or extracted from the liquid tank 14 by the first infusion pump 9 a. The liquid-phase tank 146 serving as a color development tank may also be provided with a spray passage on a side wall of the liquid-phase tank 146. Positive and negative electrodes connected to a dc power supply may be provided in the liquid phase tank 146, and a dc electric field may be applied to develop a thin layer solvent under the electric field, thereby accelerating separation of sample components on the thin layer chromatography plate 10.
In the scheme of the preferred embodiment, as shown in fig. 1,2 and 6, the automated intelligent detection instrument for thin-layer chromatography further comprises a sample application device 2 above the rotary driving device 1, the sample application device 2 and the rotary driving device 1 perform cooperative operation, the sample application device 2 is used for applying a sample to the thin-layer chromatography plate 10, the sample application device 2 comprises a transverse sliding table 21 and a longitudinal sliding table 22 fixed on the transverse sliding table 21, a sample application head 23 is fixed on the longitudinal sliding table 22, and a liquid outlet of the sample application head 23 is positioned right above the sample application platform 12 during sample application. When the thin layer chromatography plate 10 needs to be spotted, firstly, the horizontal sliding table 21 moves the longitudinal sliding table 22 to be right above the spotting platform 12, the thin layer chromatography plate 10 to be spotted is placed on the spotting platform 12, then, the longitudinal sliding table 22 drives the spotting head 23 to move downwards until the liquid outlet of the spotting head 23 is close to the thin layer chromatography plate 10, and thus, repeated accurate positioning of a sample on the thin layer chromatography plate 10 can be ensured when the spotting head 23 spots the thin layer chromatography plate 10. The longitudinal sliding table 22 on the sample application device 2 does not interfere with a series of rotation of the rotary driving device 1, and the thin layer chromatography plate 10 is applied with sample according to a set position only when the sample is applied by moving the transverse sliding table 21 from the position to be applied to the position above the rotary driving device 1.
In the preferred embodiment, as shown in fig. 1 and 2, the rotary driving device 1 and the sample application device 2 are installed in a frame 5, an image acquisition device for scanning/shooting/recording the thin layer chromatography plate 10 is arranged above the rotary driving device 1, an ultraviolet lamp 7 for irradiating the upper part of the rotary driving device 1 is further arranged at the top of the base frame 5, a recovery bin 6 for collecting the thin layer chromatography plate 10 is further arranged at the bottom of the frame 5, a first infusion pump 9a for providing developing solvent or color-developing agent for the liquid phase tank 14 is further arranged in the frame 5, and the first infusion pump 9a is preferably but not limited to a peristaltic pump, a reciprocating pump, a vacuum pump and the like. The liquid inlet of the first infusion pump 9a is connected with the developing solvent or the developer container through a pipeline, the liquid outlet is connected with the liquid inlet and outlet hole of the liquid phase groove 146 through a pipeline, the base frame 5 is internally provided with the air heater 8, and the air outlet of the air heater 8 supplies air to the thin layer chromatography plate box top surface platform 133 or the liquid phase groove box top surface platform 145 above the rotary driving device 1 through a ventilation pipeline, so that the drying of the thin layer chromatography plate 10 is accelerated.
In the preferred embodiment, the liquid inlet of the second liquid conveying pump 9b of the thin-layer chromatography automatic intelligent detection instrument is connected with the sample container through a pipeline, and the automatic sampling and sample application processes can be performed through an automatic program. In the detection of synthetic chemical reaction, a plurality of reaction vessels can be connected at the same time, and the reaction progress can be known by detecting the change of the content of a reactant or a product with time.
In a preferred embodiment, the automated intelligent detection instrument for thin layer chromatography has an automatic control system including, but not limited to, a PC computer, a PLC control board, a motion control card, a motor drive board, and image analysis and storage and sample calculation software; the automatic control system is connected with the Internet and the Internet of things, and a user controls or monitors sample detection through a PC (personal computer), a workstation and an intelligent mobile terminal.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (11)
1. The real-time on-line detection method for the thin layer chromatography automatic intelligent detection instrument is characterized in that the detection instrument is provided with a thin layer chromatography plate box for storing a plurality of thin layer chromatography plates, an unfolding system for the liquid layer chromatography plate box, a sample application platform for supporting the thin layer chromatography plates, a rotary driving device for moving the thin layer chromatography plates out of the thin layer chromatography plate box to the sample application platform and moving the thin layer chromatography plates into and out of the liquid layer chromatography plate box, a sample application device and an image capturing and analyzing device, and the detection method can realize the real-time on-line detection method by combining the operation steps of automatic sampling and thin layer chromatography analysis through the devices, and comprises the following steps:
Step1, after a thin layer chromatography plate box and a liquid phase tank box which are provided with the thin layer chromatography plate are placed into a rotary driving device, the relative heights of the thin layer chromatography plate box and the liquid phase tank box as well as a sample application platform are adjusted, the thin layer chromatography plate is inclined by rotating the rotary driving device, the thin layer chromatography plate slides out of the thin layer chromatography plate box to the sample application platform under the action of gravity and is applied with sample application through a sample application device, and then the thin layer chromatography plate slides into a liquid phase tank of the liquid phase tank box by utilizing the same principle; in the rotary driving device, the thin-layer chromatography plate box and the liquid-phase tank box are positioned at two ends of the sample application platform, openings correspond to each other, and the thin-layer chromatography plate can be moved back and forth between the thin-layer chromatography plate box and the liquid-phase tank box through the sample application platform by rotating the rotary driving device;
Step 2, injecting developing solvent into one or more liquid phase tanks of the liquid phase tank box to perform thin layer chromatography development of various functions, and extracting the developing solvent in the liquid phase tank after the development treatment is finished; the bottom of the liquid phase tank is connected with an infusion pump to inject or extract developing solution, and the liquid phase tank can inject or spray a developing agent to develop the thin-layer chromatographic plate;
Step 3, enabling the thin layer chromatographic plate to slide out of the liquid phase groove by using a rotary driving device, and enabling the thin layer chromatographic plate to enter any one of a sample application platform, a thin layer chromatographic plate box top surface platform and a liquid phase groove box top surface platform for image shooting analysis, wherein the image shooting device comprises, but is not limited to, an ultraviolet scanner, an infrared scanner, an industrial camera, ultraviolet irradiation and drying equipment;
Step 4, sliding the thin-layer chromatography plate after detection and analysis to a lower recovery bin through a recovery channel in the thin-layer chromatography plate box by using a rotary driving device, and preparing the same detection operation for the next new thin-layer chromatography plate by an automatic process;
and (3) executing the operation instructions in the steps 1 to 4 by an automatic control system to realize real-time online thin layer chromatography detection analysis.
2. The method for real-time on-line detection by using the automated thin-layer chromatography intelligent detection instrument according to claim 1, wherein the method for spotting the thin-layer chromatography plate on the spotting platform in step 1 is as follows: starting a transverse sliding table of the sample application device to horizontally move the longitudinal sliding table to the upper side of the thin layer chromatography plate to be applied with sample, downwardly moving the sample application head to the set distance of the thin layer chromatography plate to be applied with sample by the longitudinal sliding table, then applying sample to the thin layer chromatography plate to be applied with the liquid outlet of the sample application head, and resetting the sample application device to the original position after the sample application is completed.
3. The method for real-time on-line detection of thin-layer chromatography automated intelligent detection instrument according to claim 2, wherein the liquid inlet of the sample application head of the sample application device is connected with an infusion pump, the liquid sampling inlet of the infusion pump is connected with a chemical or biochemical reaction container, sampling is automatically timed, and sample application is carried out on the thin-layer chromatography plate through the sample application head.
4. The automatic intelligent detection instrument for thin-layer chromatography analysis is characterized by comprising a thin-layer chromatography plate box and a liquid-phase tank box, wherein a sample application platform is arranged between the thin-layer chromatography plate box and the liquid-phase tank box, openings of the thin-layer chromatography plate box and the liquid-phase tank box face the sample application platform, the thin-layer chromatography plate box and the liquid-phase tank box are connected with a lifting sliding table for adjusting the relative height of the thin-layer chromatography plate box and the sample application platform, the thin-layer chromatography plate box, the liquid-phase tank box and the sample application platform are synchronously driven to rotate through a rotary driving device, and the thin-layer chromatography plate is conveyed among the thin-layer chromatography plate box, the liquid-phase tank box and the sample application platform under the action of gravity;
The thin layer chromatography plate box comprises a containing mechanism capable of containing a plurality of thin layer chromatography plates, wherein the top of the containing mechanism is provided with a recycling channel of the thin layer chromatography plates, and the top of the thin layer chromatography plate box is provided with a thin layer chromatography plate box top surface platform for placing or transferring the thin layer chromatography plates;
The liquid phase tank box is formed by alternately superposing a plurality of second U-shaped plates and a plurality of second partition plates, the opening orientations of the second U-shaped plates are the same, the liquid phase tank box is used for expanding the solvent of the thin layer chromatography plate, the top of the liquid phase tank box is provided with a liquid phase tank box top surface platform for placing or transferring the thin layer chromatography plate, the bottom of a liquid phase tank in the liquid phase tank box is provided with an inlet and outlet hole for circulating expanded solvent, and the liquid phase tank of the liquid phase tank box can be used for expanding grooves or developing grooves.
5. The automated intelligent thin-layer chromatography detection instrument according to claim 4, wherein the accommodating mechanism of the thin-layer chromatography plate box is formed by alternately superposing a plurality of first U-shaped plates and a plurality of first partition plates, and openings of the plurality of first U-shaped plates face the same direction, and the thin-layer chromatography plates are stored in gaps of the U-shaped plates.
6. The automated intelligent detection instrument for thin-layer chromatography according to claim 4, wherein the rotary driving device comprises a frame, the frame is used for fixing and supporting a sample application platform, a thin-layer chromatography plate box, a liquid-phase groove box and a lifting sliding table, bearing rods are fixedly sleeved at the left end and the right end of the frame, one end of each bearing rod is connected with an output shaft of a motor, the motor drives the frame to rotate forward and backward through the bearing rods, the lifting sliding table comprises a first lifting sliding table and a second lifting sliding table, the first lifting sliding table drives the thin-layer chromatography plate box to move, and the second lifting sliding table drives the liquid-phase groove box to move.
7. The automated intelligent detection instrument for thin-layer chromatography according to claim 4, further comprising a sample application device positioned above the rotary driving device, wherein the sample application device applies an application operation to the thin-layer chromatography plate, the sample application device comprises a transverse sliding table and a longitudinal sliding table positioned on the transverse sliding table, the longitudinal sliding table is provided with an application head, and a liquid outlet of the application head is positioned right above the application platform during application.
8. The automated thin-layer chromatography intelligent detection apparatus according to any one of claims 4 to 7, further comprising a frame, wherein the rotary driving device and the sample spotting device are installed in the closable frame, an image acquisition device for capturing images of the thin-layer chromatography plate is arranged above or in front of and behind the rotary driving device, and an ultraviolet lamp for irradiating the thin-layer chromatography plate is further arranged above the frame; the bottom of the frame is also provided with a recovery bin for collecting the thin-layer chromatographic plates.
9. The automated intelligent detection instrument for thin-layer chromatography according to claim 8, wherein a first infusion pump for providing developing solvent or color-developing agent for the liquid phase tank is further arranged in the rack, a liquid inlet of the first infusion pump is connected with a container of the developing solvent or the color-developing agent through a pipeline, and a liquid outlet of the first infusion pump is connected with an inlet and outlet hole of the liquid phase tank through a pipeline; the frame is also internally provided with a second infusion pump for sampling, a liquid inlet of the second infusion pump is connected with a container of the sample through a pipeline, and a liquid outlet of the second infusion pump is connected with the sample application head through a pipeline.
10. The automated intelligent detection instrument for thin-layer chromatography according to claim 8, wherein an air heater is further arranged in the frame, and an air outlet of the air heater supplies air to the sample application platform, the top surface platform of the thin-layer chromatography plate box and the top surface platform of the liquid-phase tank box, and heating plates are further arranged on the sample application platform, the top surface platform of the thin-layer chromatography plate box and the top surface platform of the liquid-phase tank box to heat and develop the thin-layer chromatography plate.
11. The automated thin-layer chromatography intelligent detection instrument according to claim 8, further comprising an automatic control system, wherein the automatic control system can be networked and intercommunicated, and a user controls or monitors the detection instrument through a PC computer, a workstation, and an intelligent mobile terminal.
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| CN201810326395.2A CN108459133B (en) | 2018-04-12 | 2018-04-12 | Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof |
| PCT/CN2019/082255 WO2019196902A1 (en) | 2018-04-12 | 2019-04-11 | Automated intelligent detection instrument for thin-layer chromatography and real-time online detection method thereof |
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| CN201810326395.2A CN108459133B (en) | 2018-04-12 | 2018-04-12 | Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof |
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| WO2019196902A1 (en) | 2019-10-17 |
| CN108459133A (en) | 2018-08-28 |
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