CN108426757B - Medical blood cell staining method - Google Patents
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- CN108426757B CN108426757B CN201810179545.1A CN201810179545A CN108426757B CN 108426757 B CN108426757 B CN 108426757B CN 201810179545 A CN201810179545 A CN 201810179545A CN 108426757 B CN108426757 B CN 108426757B
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- 210000000601 blood cell Anatomy 0.000 title claims abstract description 23
- 238000007447 staining method Methods 0.000 title claims description 7
- 238000004043 dyeing Methods 0.000 claims abstract description 64
- 238000005406 washing Methods 0.000 claims abstract description 24
- 239000011521 glass Substances 0.000 claims abstract description 21
- 238000000861 blow drying Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 238000010186 staining Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000005507 spraying Methods 0.000 claims abstract description 10
- 238000011010 flushing procedure Methods 0.000 claims abstract description 7
- 238000007664 blowing Methods 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 15
- 238000001125 extrusion Methods 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 239000011248 coating agent Substances 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 11
- 239000007921 spray Substances 0.000 claims description 8
- 239000012192 staining solution Substances 0.000 claims description 6
- 230000002146 bilateral effect Effects 0.000 claims description 5
- 230000003139 buffering effect Effects 0.000 claims description 5
- 239000002775 capsule Substances 0.000 claims description 3
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 210000003677 hemocyte Anatomy 0.000 claims description 3
- 238000001035 drying Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of medical treatment, in particular to a medical blood cell dyeing method, which adopts an automatic dyeing device, wherein the automatic dyeing device comprises a shell, a first dye bottle, a second dye bottle, a positioning module, a dyeing module, a flushing sprayer, a blow-drying module and an operation panel, the first dye bottle is filled with a Ruhrstan's dye solution, the second dye bottle is filled with a PBS dye solution, and droppers are arranged in the first dye bottle and the second dye bottle; the positioning module is used for fixing the glass slide coated with the blood cells; the staining module is used for realizing staining of blood cells; the washing nozzle is communicated with the pure water and is used for spraying the pure water to wash the glass slide; the blow-drying module is positioned on the positioning module and is matched with the positioning module to blow-dry the glass slide; the operation panel is used for inputting the amount and interval time of dye liquor dripping and the time of rinsing. The automatic smear dyeing device can automatically realize automatic smear dyeing by adopting a preset program and a parameter input mode.
Description
Technical Field
The invention belongs to the technical field of medical treatment, and particularly relates to a medical blood cell staining method.
Background
The microbiological morphology examination in medical examination is an important means for clinical pathogen diagnosis, and many specimens such as blood, urine, sputum, leucorrhea and the like need to be smeared and stained before microscopic examination. At present, the work of a laboratory is basically finished by manual operation, and the laboratory is not beneficial to biological safety of the laboratory due to no standard operation rules. Meanwhile, the existing dyeing machine has the disadvantages of complex structure, high cost and high requirement on the skill of operators, and cannot be popularized and used in a large range.
In view of this, the medical blood cell staining method automatically realizes automatic staining of the smear by adopting a preset program and a parameter input mode, and forms a uniform operation standard.
Disclosure of Invention
In order to make up the defects of the prior art, the invention provides a medical blood cell staining method which is mainly used for forming a set of standard blood cell staining operation rules and automatically realizing the staining of blood cells. According to the invention, the positioning module, the staining module, the washing nozzle and the blow-drying module are matched with each other, the staining work of blood cells is automatically completed according to the same standard operation rule by adopting a preset program and a parameter input mode, the labor intensity is low, and the consistency of sample treatment is good.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a medical blood cell dyeing method, which adopts an automatic dyeing device, wherein the automatic dyeing device comprises a shell, a first dye solution bottle, a second dye solution bottle, a positioning module, a dyeing module, a flushing nozzle, a blow-drying module and an operation panel, wherein a cavity is arranged in the shell, positioning grooves are symmetrically arranged at the left and the right of the bottom of the shell, an operation hole communicated with the outside is arranged in the middle of the bottom of the shell, and the shell is fixed on the ground through support legs; the first dye bottle and the second dye bottle are respectively placed in the positioning groove of the shell, the first dye bottle is filled with the Ruhrstan's dye, the second dye bottle is filled with the PBS dye, and droppers are respectively arranged in the first dye bottle and the second dye bottle; the positioning module is positioned below the shell and used for fixing the glass slide coated with blood cells; the staining module is positioned in the shell and used for staining blood cells; the washing nozzle is communicated with the pure water and is used for spraying the pure water to wash the glass slide; the blow-drying module is positioned on the positioning module and is matched with the positioning module to blow-dry the glass slide; the operation panel is used for inputting the number and interval time of dye liquor dripping and the time of flushing;
the method comprises the following steps:
the method comprises the following steps: parameters such as the number of dye drops, interval time, washing time and the like are input through an operation panel;
step two: placing the glass slide coated with the hemocytes in a positioning module, and clamping the glass slide by the positioning module to realize positioning;
step three: the dyeing module moves to the upper part of the first dye bottle, and a dropper in the first dye bottle is clamped up by the dyeing module;
step four: the dyeing module with the dropper moves to the middle position in the shell;
step five: the dyeing module drops the Ruhrstan's dye solution on the coating one by one according to preset parameters;
step six: the dyeing module repeats the third step to the fifth step, and the PBS dye solution in the dye solution bottle II is dripped on the coating, and stands for 30 min;
step seven: the washing nozzle slowly sprays pure water to wash the smear for 3 min;
step eight: the drying module blows natural wind to dry the smear quickly.
The part of the clamping arm (55) in the third step for clamping the dropper is a glass tube part of the dropper.
And in the fifth step, the Swiss stain is dripped into the smear and is placed for 1-2 min.
The addition amount of the Reye staining solution is that the Reye staining solution fully covers the surface of the smear.
In the sixth step, the PBS dye solution is slowly added to the coated piece one drop by one drop and is kept stand for 30 min.
In the seventh step, the smear is washed by pure water for at least 3 min.
The positioning module comprises an object stage, a clamping block, a first screw rod and a fixed motor, the object stage is horizontally arranged on the support legs and is positioned below the shell, and two sections of sliding chutes are horizontally arranged on the upper surface of the object stage in a bilateral symmetry mode; the number of the clamping blocks is two, and the clamping blocks are respectively positioned in the two sliding grooves of the objective table; the first screw rod is arranged in the sliding chute of the objective table, the screw threads of the first screw rod in the two sections of sliding chutes have opposite rotating directions, the clamping block is connected with the first screw rod through the screw threads, and the clamping block can horizontally slide on the first screw rod; the fixed motor is arranged in the objective table and connected with the first screw rod. During operation, place the smear on the objective table, fixed motor drives screw rod one and rotates, and the clamp splice moves towards the centre under the drive of screw rod one and draws close, presss from both sides the smear tight, has realized the location of smear.
The dyeing module comprises a second screw rod, a sliding block, a clamping cylinder, a first telescopic cylinder, a clamping arm, a second telescopic cylinder, an extrusion block and a dyeing motor, wherein the second screw rod is horizontally arranged at the top end of the shell, and the second screw rod is positioned in the shell; the sliding block is installed on the second screw rod through threaded connection; the clamping cylinder is horizontally arranged at the lower end of the sliding block; the number of the first telescopic cylinders is two, the first telescopic cylinders are bilaterally and symmetrically vertically arranged on the clamping cylinders, and the clamping cylinders drive the first telescopic cylinders to move in the same direction or in the opposite direction; the number of the clamping arms is two, the clamping arms are horizontally fixed at the bottom end of the first telescopic cylinder and are used for clamping the dropper; the number of the second telescopic cylinders is two, and the second telescopic cylinders are horizontally arranged at the lower ends of the first telescopic cylinders; the extrusion block is fixed at the end part of the telescopic cylinder II and is used for extruding the rubber cap part of the dropper; the dyeing motor is installed on the shell and used for driving the second screw rod to rotate. Then, the dyeing motor drives the screw rod II to rotate, the sliding block moves leftwards to be above the first dye bottle along with the movement of the screw rod II, the telescopic cylinder I drives the clamping arm to move downwards to clamp the dropper, after the dropper is moved out of the first dye bottle by the clamping arm, the dyeing motor rotates reversely, the sliding block moves to the middle position of the shell, the dropper synchronously moves to the middle position of the shell, and the telescopic cylinder moves downwards to enable the tip of the dropper to be close to the smear; and then the telescopic cylinder II moves relatively to enable the extrusion block to extrude the rubber cap of the dropper, the dyeing liquor of Ruehrlington in the dropper is dripped on the coating, and the dripped quantity and interval time are controlled by the input parameters.
The washing nozzle is arranged on the clamping block and can move along with the clamping block, the nozzle is adjusted, the washing spraying moves along with the clamping block, smears with different sizes can be adapted, and the best washing effect is guaranteed.
The blow-drying module comprises a rubber bag, a spring, a bracket, a blowing nozzle and an air storage tank, wherein the rubber bag is fixed on the left side wall and the right side wall of the shell, the rubber bag and the clamping cylinder are positioned at the same height, a cavity is arranged in the rubber bag, an air inlet and an air outlet are arranged on the rubber bag, a one-way valve is arranged at the position of the air inlet, and the air outlet is communicated with the air storage tank; the spring is positioned in the rubber bag and used for enabling the wanted capsule to recover the original state; the bracket is fixed on the clamping block; the air blowing nozzle is installed on the support, the angle of the air blowing nozzle on the support can be adjusted, the air blowing nozzle is connected with the air storage tank, and the air blowing nozzle is used for spraying air to the smear. The in-process that removes about die clamping cylinder, die clamping cylinder and rubber bag strike mutually, have played the effect of buffering to die clamping cylinder, and the rubber bag is because compressed and with inside air escape simultaneously, then the reconversion under the effect of spring has realized supplying air to the gas holder, and the inside air of gas holder is through blowing nozzle blowout, realizes drying fast of smear.
The invention has the beneficial effects that:
1. according to the medical blood cell dyeing method, the Switzerland dye liquor and the PBS dye liquor are adopted for dyeing, so that the dyeing efficiency is high, the dyeing effect is good, the dyeing effect lasts for a long time, and the subsequent observation is facilitated.
2. According to the medical blood cell dyeing method, the automatic dyeing device can automatically complete dyeing work by inputting parameters through the control panel, standard operation can be realized, and the dyeing effect is ensured; meanwhile, the labor intensity of medical staff is reduced, and the working efficiency is high.
3. According to the medical blood cell staining method, the blow-drying module collects air by utilizing the movement of the staining module, and then the air blowing nozzle sprays the air to dry the smear, so that the buffering of the staining module is realized, the energy utilization is improved, and the use cost is reduced.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of the dyeing method of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a cross-sectional view A-A of FIG. 1;
in the figure: the device comprises a shell 1, a first dye bottle 2, a second dye bottle 3, a positioning module 4, a dyeing module 5, a washing spray head 6, a blow-drying module 7, an operation panel 8, a support leg 11, an object stage 41, a clamping block 42, a first screw 43, a fixed motor 44, a second screw 51, a sliding block 52, a clamping cylinder 53, a first telescopic cylinder 54, a clamping arm 55, a second telescopic cylinder 56, an extrusion block 57, a dyeing motor 58, a rubber bag 71, a spring 72, a support 73 and a blowing nozzle 74.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 3, the medical blood cell dyeing method of the present invention adopts an automatic dyeing apparatus, the automatic dyeing apparatus includes a housing 1, a first dyeing liquid bottle 2, a second dyeing liquid bottle 3, a positioning module 4, a dyeing module 5, a washing nozzle 6, a blow-drying module 7 and an operation panel 8, a cavity is arranged in the housing 1, positioning slots are symmetrically arranged at the bottom of the housing 1, an operation hole is arranged at the middle position of the bottom of the housing 1 to communicate with the outside, and the housing 1 is fixed on the ground through a support leg 11; the first dye bottle 2 and the second dye bottle 3 are respectively placed in the positioning grooves of the shell 1, the first dye bottle 2 is filled with the Ruhrstan's dye, the second dye bottle 3 is filled with the PBS dye, and droppers are respectively arranged in the first dye bottle 2 and the second dye bottle 3; the positioning module 4 is positioned below the shell 1, and the positioning module 4 is used for fixing the glass slide coated with blood cells; the staining module 5 is positioned in the shell 1, and the staining module 5 is used for staining blood cells; the washing nozzle 6 is communicated with pure water, and the washing nozzle 6 is used for spraying the pure water to wash the glass slide; the blow-drying module 7 is positioned on the positioning module 4, and the blow-drying module 7 is matched with the positioning module 4 to realize blow-drying of the glass slide; the operation panel 8 is used for inputting the number and interval time of dye liquor dripping and the time of flushing;
the method comprises the following steps:
the method comprises the following steps: parameters such as the number of dye drops, the interval time, the rinsing time and the like are input through the operation panel 8;
step two: placing the glass slide coated with the hemocytes on a positioning module 4, and clamping the glass slide by the positioning module 4 to realize positioning;
step three: the dyeing module 5 moves above the first dye solution bottle 2, and the dropper in the first dye solution bottle 2 is clamped up by the dyeing module 5;
step four: the dyeing module 5 carrying the dropper moves to an intermediate position inside the casing 1;
step five: the dyeing module 5 is used for dripping the Ruhrstan's dye solution on the coating one drop by one drop according to preset parameters;
step six: the dyeing module 5 repeats the third step to the fifth step, and the PBS dye solution in the dye solution bottle II 3 is dripped on the coating, and stands for 30 min;
step seven: the washing nozzle 6 slowly sprays pure water to wash the smear for 3 min;
step eight: the drying module 7 blows natural wind to dry the smear quickly.
The part of the clamping arm (55) in the third step for clamping the dropper is a glass tube part of the dropper.
And in the fifth step, the Swiss stain is dripped into the smear and is placed for 1-2 min.
The addition amount of the Reye staining solution is that the Reye staining solution fully covers the surface of the smear.
In the sixth step, the PBS dye solution is slowly added to the coated piece one drop by one drop and is kept stand for 30 min.
In the seventh step, the smear is washed by pure water for at least 3 min.
The positioning module 4 comprises an object stage 41, a clamping block 42, a first screw 43 and a fixed motor 44, wherein the object stage 41 is horizontally arranged on the support leg 11, the object stage 41 is positioned below the shell 1, and two sections of sliding grooves are horizontally arranged on the upper surface of the object stage 41 in a bilateral symmetry manner; the number of the clamping blocks 42 is two, and the clamping blocks 42 are respectively positioned in the two sliding grooves of the objective table 41; the first screw 43 is arranged in the sliding chute of the objective table 41, the screw threads of the first screw 43 in the two sliding chutes are opposite in rotating direction, the clamping block 42 is in threaded connection with the first screw 43, and the clamping block 42 can horizontally slide on the first screw 43; the fixed motor 44 is arranged in the object stage 41, and the fixed motor 44 is connected with the first screw 43. During operation, place the smear on objective table 41, fixed motor 44 drives screw rod 43 and rotates, and clamp splice 42 moves towards the centre under the drive of screw rod 43 and draws close the smear tight, has realized the location of smear.
The dyeing module 5 comprises a second screw 51, a sliding block 52, a clamping cylinder 53, a first telescopic cylinder 54, a clamping arm 55, a second telescopic cylinder 56, an extrusion block 57 and a dyeing motor 58, wherein the second screw 51 is horizontally arranged at the top end of the shell 1, and the second screw 51 is positioned in the shell 1; the sliding block 52 is installed on the second screw rod 51 through threaded connection; the clamping cylinder 53 is horizontally arranged at the lower end of the sliding block 52; the number of the first telescopic cylinders 54 is two, the first telescopic cylinders 54 are vertically arranged on the clamping cylinder 53 in a bilateral symmetry mode, and the clamping cylinder 53 drives the first telescopic cylinders 54 to move in the same direction or in the opposite direction; the number of the clamping arms 55 is two, the clamping arms 55 are horizontally fixed at the bottom end of the first telescopic cylinder 54, and the clamping arms 55 are used for clamping a dropper; the number of the second telescopic cylinders 56 is two, and the second telescopic cylinders 56 are horizontally arranged at the lower ends of the first telescopic cylinders 54; the extrusion block 57 is fixed at the end part of the second telescopic cylinder 56, and the extrusion block 57 is used for extruding the rubber cap part of the dropper; the dyeing motor 58 is installed on the housing 1, and the dyeing motor 58 is used for driving the second screw 51 to rotate. Then, the dyeing motor 58 drives the screw rod II 51 to rotate, the sliding block 52 moves leftwards to the upper side of the dye liquor bottle I2 along with the movement of the screw rod II 51, the telescopic cylinder I54 drives the clamping arm 55 to move downwards to clamp the dropper, after the dropper is moved out of the dye liquor bottle I2 by the clamping arm 55, the dyeing motor 58 rotates reversely, the sliding block 52 moves to the middle position of the shell 1, the dropper synchronously moves to the middle position of the shell 1, and the telescopic cylinder moves downwards to enable the tip of the dropper to be close to the smear; and then the second telescopic cylinder 56 moves relatively to enable the extrusion block 57 to extrude the rubber cap of the dropper, the dyeing liquor in the dropper is dripped on the coating, and the dripped quantity and interval time are controlled by the input parameters.
The washing spray head 6 is arranged on the clamping block 42, the washing spray head 6 can move along with the clamping block 42, the spray head can be adjusted, the washing spray coating moves along with the clamping block 42, smears with different sizes can be adapted, and the best washing effect is guaranteed.
The blow-drying module 7 comprises a rubber bag 71, a spring 72, a bracket 73, a blowing nozzle 74 and an air storage tank, wherein the rubber bag 71 is fixed on the left and right side walls of the shell 1, the rubber bag 71 and the clamping cylinder 53 are positioned at the same height, a cavity is arranged in the rubber bag 71, the rubber bag 71 is provided with an air inlet and an air outlet, the air inlet is provided with a one-way valve, and the air outlet is communicated with the air storage tank; the spring 72 is positioned in the rubber bag 71, and the spring 72 is used for restoring the wanted capsule; the bracket 73 is fixed on the clamping block 42; the air blowing nozzle 74 is arranged on the bracket 73, the angle of the air blowing nozzle 74 on the bracket 73 can be adjusted, the air blowing nozzle 74 is connected with an air storage tank, and the air blowing nozzle 74 is used for spraying air to the smear. In the process that the clamping cylinder 53 moves left and right, the clamping cylinder 53 collides with the rubber bag 71, the effect of buffering is achieved on the clamping cylinder 53, meanwhile, the rubber bag 71 discharges the air inside due to compression, then the rubber bag is restored under the action of the spring 72, the air is supplied to the air storage tank, the air inside the air storage tank is sprayed out through the air blowing nozzle 74, and the smear is dried quickly.
The specific working process is as follows:
when the device works, a smear is placed on the object stage 41, the first screw 43 is driven by the fixed motor 44 to rotate, the clamping blocks 42 move towards the middle under the drive of the first screw 43, the smear is clamped, and the positioning of the smear is realized; the flushing and spraying can be adapted to smears with different sizes along with the movement of the clamping block 42, and the optimal flushing effect is ensured.
Then, the dyeing motor 58 drives the screw rod II 51 to rotate, the sliding block 52 moves leftwards to the upper side of the dye liquor bottle I2 along with the movement of the screw rod II 51, the telescopic cylinder I54 drives the clamping arm 55 to move downwards to clamp the dropper, after the dropper is moved out of the dye liquor bottle I2 by the clamping arm 55, the dyeing motor 58 rotates reversely, the sliding block 52 moves to the middle position of the shell 1, the dropper synchronously moves to the middle position of the shell 1, and the telescopic cylinder moves downwards to enable the tip of the dropper to be close to the smear; and then the second telescopic cylinder 56 moves relatively to enable the extrusion block 57 to extrude the rubber cap of the dropper, the dyeing liquor of Ruehr in the dropper is dripped on the coating, the dripped quantity and interval time are controlled by the input parameters, and the steps are repeated to drip the PBS dyeing liquor on the coating.
In the process that the clamping cylinder 53 moves left and right, the clamping cylinder 53 collides with the rubber bag 71, the effect of buffering is achieved on the clamping cylinder 53, meanwhile, the rubber bag 71 discharges the air inside due to compression, then the rubber bag is restored under the action of the spring 72, the air is supplied to the air storage tank, the air inside the air storage tank is sprayed out through the air blowing nozzle 74, and the smear is dried quickly.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. A medical blood cell staining method is characterized in that: the dyeing method comprises the following steps:
the method comprises the following steps: inputting parameters of the number of the dye drops, the interval time and the rinsing time on an operation panel (8);
step two: placing the glass slide coated with the hemocytes on an object stage (41) of a positioning module (4), and clamping the glass slide by a clamping block (42) to realize positioning;
step three: the clamping arm (55) of the dyeing module (5) moves to the upper part of the first dye bottle (2), and the dropper in the first dye bottle (2) is clamped up by the clamping arm (55);
step four: the dropper is clamped by the clamping arm (55) and moves to the middle position in the shell (1);
step five: according to preset parameters, an extrusion block (57) extrudes a rubber cap of a dropper momentarily, the Reye staining solution is dripped on the smear one by one, and the Reye staining solution is placed for 1-2min after being dripped on the smear;
step six: repeating the third step to the fifth step, dripping the PBS dye solution in the dye solution bottle II (3) on the coating, slowly dripping the PBS dye solution on the coating, and standing for 30 min;
step seven: the washing nozzle (6) slowly sprays pure water to wash the smear for at least 3 min;
step eight: natural wind is blown out by the blowing nozzle (74) to dry the smear quickly;
the part of the clamping arm (55) in the third step for clamping the dropper is a glass tube part of the dropper;
the number of the drops of the Swiss stain is that the Swiss stain fully covers the surface of the smear;
a cavity is formed in the shell (1), positioning grooves are symmetrically formed in the left and right of the bottom of the shell (1), an operation hole is formed in the middle of the bottom of the shell (1) and communicated with the outside, and the shell (1) is fixed on the ground through support legs (11); a first dye bottle (2) and a second dye bottle (3) are respectively placed in the positioning groove of the shell (1), the first dye bottle (2) is filled with the Ruhrstan's dye, the second dye bottle (3) is filled with the PBS dye, and droppers are respectively arranged in the first dye bottle (2) and the second dye bottle (3); the positioning module (4) is positioned below the shell (1), and the positioning module (4) is used for fixing the glass slide coated with blood cells; the staining module (5) is positioned in the shell (1), and the staining module (5) is used for staining blood cells; the washing nozzle (6) is communicated with pure water, and the washing nozzle (6) is used for spraying the pure water to wash the glass slide; the blow-drying module (7) is positioned on the positioning module (4), and the blow-drying module (7) is matched with the positioning module (4) to realize blow-drying of the glass slide; the operation panel (8) is used for inputting the number and interval time of dye liquor dripping and the time of flushing;
the positioning module (4) comprises an object stage (41), a clamping block (42), a first screw rod (43) and a fixed motor (44), the object stage (41) is horizontally arranged on the support leg (11), the object stage (41) is positioned below the shell (1), and two sections of sliding chutes are horizontally arranged on the upper surface of the object stage (41) in a bilateral symmetry manner; the number of the clamping blocks (42) is two, and the clamping blocks (42) are respectively positioned in the two sliding grooves of the objective table (41); the first screw rod (43) is arranged in a sliding groove of the objective table (41), the screw threads of the first screw rod (43) in the two sliding grooves are opposite in rotating direction, the clamping block (42) is connected with the first screw rod (43) through screw threads, and the clamping block (42) can horizontally slide on the first screw rod (43); the fixed motor (44) is arranged in the objective table (41), and the fixed motor (44) is connected with the first screw rod (43); when the device works, a smear is placed on the object stage (41), the first screw rod (43) is driven to rotate by the fixed motor (44), the clamping block (42) moves towards the middle under the drive of the first screw rod (43) to clamp the smear, and the smear is positioned;
the dyeing module (5) comprises a second screw rod (51), a sliding block (52), a clamping cylinder (53), a first telescopic cylinder (54), a clamping arm (55), a second telescopic cylinder (56), an extrusion block (57) and a dyeing motor (58), wherein the second screw rod (51) is horizontally arranged at the top end of the shell (1), and the second screw rod (51) is positioned in the shell (1); the sliding block (52) is installed on the second screw rod (51) through threaded connection; the clamping cylinder (53) is horizontally arranged at the lower end of the sliding block (52); the number of the first telescopic cylinders (54) is two, the first telescopic cylinders (54) are vertically arranged on the clamping cylinder (53) in a bilateral symmetry mode, and the clamping cylinder (53) drives the first telescopic cylinders (54) to move in the same direction or in the opposite direction; the number of the clamping arms (55) is two, the clamping arms (55) are horizontally fixed at the bottom end of the first telescopic cylinder (54), and the clamping arms (55) are used for clamping a dropper; the number of the second telescopic cylinders (56) is two, and the second telescopic cylinders (56) are horizontally arranged at the lower ends of the first telescopic cylinders (54); the extrusion block (57) is fixed at the end part of the second telescopic cylinder (56), and the extrusion block (57) is used for extruding the rubber cap part of the dropper; the dyeing motor (58) is arranged on the shell (1), and the dyeing motor (58) is used for driving the second screw rod (51) to rotate; then, a dyeing motor (58) drives a screw rod II (51) to rotate, a sliding block (52) moves leftwards to the upper side of a dye bottle I (2) along with the movement of the screw rod II (51), a telescopic cylinder I (54) drives a clamping arm (55) to move downwards to clamp a dropper, after the dropper is moved out of the dye bottle I (2) by the clamping arm (55), the dyeing motor (58) rotates reversely, the sliding block (52) moves to the middle position of a shell (1), the dropper synchronously moves to the middle position of the shell (1), and the telescopic cylinder moves downwards to enable a tip of the dropper to be close to a smear; then the telescopic cylinder II (56) moves relatively to enable the extrusion block (57) to extrude the rubber cap of the dropper, the Ruhrstan's dye liquor in the dropper is dripped on the coating, and the dripped quantity and interval time are controlled by the input parameters;
the washing nozzle (6) is arranged on the clamping block (42), the washing nozzle (6) can move along with the clamping block (42) to realize adjustment of the nozzle, the washing nozzle (6) moves along with the clamping block (42), and can adapt to smears of different sizes, so that the optimal washing effect is ensured;
the blow-drying module (7) comprises a rubber bag (71), a spring (72), a support (73), a blowing nozzle (74) and an air storage tank, wherein the rubber bag (71) is fixed on the left side wall and the right side wall of the shell (1), the rubber bag (71) and the clamping cylinder (53) are at the same height, a cavity is arranged in the rubber bag (71), an air inlet and an air outlet are formed in the rubber bag (71), a one-way valve is arranged at the position of the air inlet, and the air outlet is communicated with the air storage tank; the spring (72) is positioned in the rubber bag (71), and the spring (72) is used for restoring the wanted capsule; the bracket (73) is fixed on the clamping block (42); the air blowing nozzle (74) is installed on the bracket (73), the angle of the air blowing nozzle (74) on the bracket (73) can be adjusted, the air blowing nozzle (74) is connected with the air storage tank, and the air blowing nozzle (74) is used for spraying air to the smear; in the process that the clamping cylinder (53) moves left and right, the clamping cylinder (53) and the rubber bag (71) are impacted mutually, the effect of buffering is achieved on the clamping cylinder (53), meanwhile, the rubber bag (71) discharges the air inside due to compression, then the rubber bag is restored to the original state under the action of the spring (72), air is supplied to the air storage tank, the air inside the air storage tank is sprayed out through the air blowing nozzle (74), and the smear is dried quickly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810179545.1A CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810179545.1A CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
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| CN108426757A CN108426757A (en) | 2018-08-21 |
| CN108426757B true CN108426757B (en) | 2020-10-09 |
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| CN201810179545.1A Active CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
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| CN110631882B (en) * | 2019-10-22 | 2022-01-28 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204718U (en) * | 2011-09-22 | 2012-04-25 | 汕头大学医学院第一附属医院 | Slide specimen dyeing machine |
| CN103267668A (en) * | 2013-05-02 | 2013-08-28 | 刘洪莉 | Configurable multifunctional dyeing instrument |
| CN207964417U (en) * | 2018-01-23 | 2018-10-12 | 杭州市余杭区中医院 | A kind of device that can be dyed in batches |
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2018
- 2018-03-05 CN CN201810179545.1A patent/CN108426757B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204718U (en) * | 2011-09-22 | 2012-04-25 | 汕头大学医学院第一附属医院 | Slide specimen dyeing machine |
| CN103267668A (en) * | 2013-05-02 | 2013-08-28 | 刘洪莉 | Configurable multifunctional dyeing instrument |
| CN207964417U (en) * | 2018-01-23 | 2018-10-12 | 杭州市余杭区中医院 | A kind of device that can be dyed in batches |
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| CN108426757A (en) | 2018-08-21 |
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