CN108426757A - A kind of Medical blood cell dyeing method - Google Patents
A kind of Medical blood cell dyeing method Download PDFInfo
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- CN108426757A CN108426757A CN201810179545.1A CN201810179545A CN108426757A CN 108426757 A CN108426757 A CN 108426757A CN 201810179545 A CN201810179545 A CN 201810179545A CN 108426757 A CN108426757 A CN 108426757A
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- solution bottle
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- smear
- blood cell
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- 238000004043 dyeing Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 26
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 18
- 238000011010 flushing procedure Methods 0.000 claims abstract description 26
- 239000011521 glass Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000004040 coloring Methods 0.000 claims abstract description 7
- 238000007664 blowing Methods 0.000 claims description 16
- 238000010422 painting Methods 0.000 claims description 12
- 239000002775 capsule Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 3
- 238000010186 staining Methods 0.000 abstract description 34
- 238000001035 drying Methods 0.000 abstract description 18
- 238000005507 spraying Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 8
- 239000003381 stabilizer Substances 0.000 description 5
- 230000003139 buffering effect Effects 0.000 description 4
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 238000011022 operating instruction Methods 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 201000005630 leukorrhea Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 206010046901 vaginal discharge Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to field of medical technology, specifically a kind of Medical blood cell dyeing method, the colouring method uses automatic staining device, the automatic staining device includes shell, color solution bottle one, color solution bottle two, locating module, staining modules, Flushing nozzle, drying module and operation panel, Wright's stain is contained in color solution bottle one, PBS dye liquors are contained in color solution bottle two, and dropper is both provided in color solution bottle one and color solution bottle two；Locating module is for the fixed glass slide for being coated with haemocyte；Staining modules for realizing haemocyte dyeing；Flushing nozzle is connected with pure water, and Flushing nozzle is rinsed glass slide for spraying pure water；Drying module is located on locating module, and drying module matches the drying for realizing glass slide with locating module；Operation panel is used to input the quantity that dye liquor is added dropwise and interval time and the time of flushing.The present invention can pre-set programs, take input parameter by way of realize the automatic staining of smear automatically.
Description
Technical field
The invention belongs to field of medical technology, specifically a kind of Medical blood cell dyeing method.
Background technology
In medical test microbial morphology inspection be clinical pathogens diagnosis important means, do microexamination it
Before, many samples such as blood, urine, sputum, leukorrhea etc. are required for carrying out smear, dyeing.This work of laboratory at present is basic
It is completed by manual operation, no standard practice instructions is unfavorable for Laboratory biosafety.Meanwhile existing overflow dyeing machine it is complicated,
It is of high cost, it is high to the skill set requirements of personnel's operation, it can not promote the use of on a large scale.
In consideration of it, a kind of Medical blood cell dyeing method of the present invention, by pre-set programs, takes input parameter
Mode realizes the automatic staining of smear automatically, forms unified operation standard.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of Medical blood cell dyeing method, and the present invention is main
It is used to form standard set blood cell staining operating instruction, the automatic dyeing for realizing haemocyte.The present invention by locating module,
Staining modules, Flushing nozzle and drying module cooperate with each other, pre-set programs, take input parameter by way of, press
According to uniform operating instruction, it is automatically performed the dyeing work of haemocyte, labor intensity is low, sample process consistency is good.
The technical solution adopted by the present invention to solve the technical problems is：A kind of medical blood cell staining side of the present invention
Method, the colouring method use automatic staining device, which includes shell, color solution bottle one, color solution bottle two, positioning mould
Block, staining modules, Flushing nozzle, drying module and operation panel, the enclosure are provided with cavity, outer casing bottom or so pair
Title is provided with locating slot, and outer casing bottom centre position is provided with handle hole and leads to the external world, and shell is fixed on ground by stabilizer blade；Institute
It states color solution bottle one and color solution bottle two is respectively placed in the locating slot of shell, Wright's stain, color solution bottle two are contained in color solution bottle one
Interior splendid attire PBS dye liquors are both provided with dropper in color solution bottle one and color solution bottle two；The locating module is located at below shell, positioning
Module is for the fixed glass slide for being coated with haemocyte；The staining modules module is located in shell, staining modules for realizing
The dyeing of haemocyte；The Flushing nozzle is connected with pure water, and Flushing nozzle is rinsed glass slide for spraying pure water；Institute
It states drying module to be located on locating module, drying module matches the drying for realizing glass slide with locating module；The operating surface
Plate is used to input the quantity that dye liquor is added dropwise and interval time and the time of flushing；
This method comprises the following steps：
Step 1：The parameters such as dye number of drops, interval time and the time of flushing are inputted by operation panel；
Step 2：The glass slide for being coated with haemocyte is positioned over locating module, and locating module clamps glass slide, realizes
Positioning；
Step 3：Staining modules are moved to one top of color solution bottle, and staining modules pick up the dropper in color solution bottle one；
Step 4：The staining modules that carry dropper are moved to the centre position of enclosure；
Step 5：Wright's stain is drop by drop added drop-wise to painting on piece by staining modules according to pre-set parameter；
Step 6：Staining modules repeat step 3-five and the PBS dye liquors in color solution bottle two are added drop-wise to painting on piece, stand
30min；
Step 7：Flushing nozzle slowly sprays pure water and is rinsed to smear, washing time 3min；
Step 8：Drying module blowout natural wind makes smear rapid airing.
The position of clamp arm (55) clamping dropper in the step 3 is the glass tube section of dropper.
After Wright's stain is added drop-wise to smear in the step 5,1-2min is placed.
The quantity that the Wright's stain is added dropwise is that Wright's stain fully covers on smear surface.
PBS dye liquors are slowly drop by drop added to painting on piece in the step 6, stand 30min.
Time of the smear through pure water rinsing at least 3min in the step 7.
The locating module includes that objective table, fixture block, screw rod one and fixed motor, the objective table are horizontally set on stabilizer blade
On, objective table is located at the lower section of shell, and symmetrical be horizontally disposed in objective table upper surface has two sections of sliding slots；The fixture block quantity is
Two, fixture block is located in two sliding slot of objective table；The screw rod one is set in the sliding slot of objective table, and screw rod one is located at two sections of cunnings
For thread rotary orientation in slot on the contrary, fixture block is connected through a screw thread with screw rod one, fixture block can the horizontal sliding on screw rod one；The fixation
Electric machine built-in is connected in objective table, fixed motor with screw rod one.When work, smear is placed on objective table, fixed motor
Screw rod one is driven to rotate, fixture block is moved toward one another to centre under the drive of screw rod one and drawn close, smear is clamped, and realizes smear
Positioning.
The staining modules include screw rod two, sliding block, clamping cylinder, telescopic cylinder one, clamp arm, telescopic cylinder two, squeeze
Block and dyeing motor, the screw rod two are horizontally placed on the top of shell, and screw rod two is located in shell；The sliding block passes through screw thread
Connection is mounted on screw rod two；The clamping cylinder is horizontally arranged at the lower end of sliding block；One quantity of the telescopic cylinder is two, is stretched
Contracting cylinder one is symmetrical to be vertically installed on clamping cylinder, and clamping cylinder drives telescopic cylinder one in the same direction or counter motion；
The clamp arm quantity is two, and clamp arm is horizontally fixed on the bottom end of telescopic cylinder one, and clamp arm is for being clamped dropper；The telescopic cylinder
Two quantity are two, and telescopic cylinder two is horizontally set on the lower end of telescopic cylinder one；The crushing block is fixed on telescopic cylinder two
End, crushing block are used to squeeze the Capsule part of dropper；On the shell, dyeing motor is for driving spiral shell for the dyeing motor installation
Bar two rotates.Then, dyeing motor drives screw rod two to rotate, and sliding block is moved to the left to color solution bottle one with the movement of screw rod two
Top, telescopic cylinder one drives clamp arm to move downward and picks up dropper, and after dropper removes color solution bottle one by clamp arm, dyeing motor is anti-
To rotation, sliding block is moved to the centre position of shell, and dropper synchronizing moving is moved downward to shell centre position, telescopic cylinder,
Make the sharp mouth of dropper close to smear；Then two relative motion of telescopic cylinder makes crushing block squeeze the Capsule of dropper, will drip
Wright's stain in pipe is added drop-wise to painting on piece, the quantity of the state modulator dropwise addition of input and interval time.
The Flushing nozzle is set on fixture block, and Flushing nozzle can be moved with fixture block, realizes the adjusting to nozzle, punching
Spraying is washed as fixture block moves, can adapt to different size of smear, guarantee reaches best flushing effect.
The drying module includes rubber bag tank, spring, holder, blowing nozzle and air accumulator, and the rubber bag tank is fixed on shell
Left and right sidewall on, rubber bag tank and clamping cylinder are in sustained height, and rubber bag tank is internally provided with cavity, is provided on rubber bag tank
Air inlet and gas outlet, air inlet position have check valve, gas outlet to be communicated with air accumulator；The spring is located in rubber bag tank, bullet
Spring thinks that capsule restores to the original state for making；The holder is fixed on fixture block；The blowing nozzle is rack-mount, and blowing nozzle is propping up
Angle on frame can be adjusted, and blowing nozzle is connected with air accumulator, and blowing nozzle is used to spray air to smear.In clamping cylinder or so
In mobile process, clamping cylinder is mutually hit with rubber bag tank, plays the effect of buffering to clamping cylinder, at the same rubber bag tank because
Internal air is discharged by compression, is then restored to the original state under the action of the spring, realizes to air accumulator and supplies air, storage
Air inside gas tank is sprayed by blowing nozzle, realizes the rapid airing of smear.
The beneficial effects of the invention are as follows：
1. a kind of Medical blood cell dyeing method of the present invention, the present invention is carried out using Wright's stain and PBS dye liquors
Dyeing, staining efficiency is high, coloring is good, and coloring duration is long, is convenient for subsequent observation.
2. a kind of Medical blood cell dyeing method of the present invention, the automatic staining device are inputted by control panel
Parameter one can be automatically performed dyeing work, can realize standard operation, ensure the effect of dyeing；Meanwhile reducing medical matters
The labor intensity of staff, work efficiency is high.
3. a kind of Medical blood cell dyeing method of the present invention, the drying module is real using the movement of staining modules
The acquisition of existing air, then realization is sprayed by blowing nozzle, smear is dried, the buffering of staining modules is not only realized, but also improve
The utilization of the energy, reduces use cost.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
The flow chart of the colouring method of the present invention of Fig. 1；
Fig. 2 is the front view of the present invention；
Fig. 3 is A-A sectional views in Fig. 1；
In figure：Shell 1, color solution bottle 1, color solution bottle 23, locating module 4, staining modules 5, Flushing nozzle 6, drying module
7, operation panel 8, stabilizer blade 11, objective table 41, fixture block 42, screw rod 1, fixed motor 44, screw rod 2 51, sliding block 52, clamping gas
Cylinder 53, telescopic cylinder 1, clamp arm 55, telescopic cylinder 2 56, crushing block 57, dyeing motor 58, rubber bag tank 71, spring 72, branch
Frame 73, blowing nozzle 74.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As shown in Figure 1 to Figure 3, a kind of Medical blood cell dyeing method of the present invention, the colouring method is using automatic dye
Color device, the automatic staining device include shell 1, color solution bottle 1, color solution bottle 23, locating module 4, staining modules 5, rinse spray
First 6, it dries up module 7 and operation panel 8, the shell 1 is internally provided with cavity, 1 bottom of shell is symmetrically set with positioning
Slot, 1 bottom centre position of shell are provided with handle hole and lead to the external world, and shell 1 is fixed on ground by stabilizer blade 11；The color solution bottle
1 and color solution bottle 23 be respectively placed in the locating slot of shell 1, Wright's stain is contained in color solution bottle 1, is contained in color solution bottle 23
PBS dye liquors are filled, dropper is both provided in color solution bottle 1 and color solution bottle 23；The locating module 4 is located at 1 lower section of shell, positioning
Module 4 is for the fixed glass slide for being coated with haemocyte；5 module of the staining modules is located in shell 1, and staining modules 5 are used for
Realize the dyeing of haemocyte；The Flushing nozzle 6 is connected with pure water, and Flushing nozzle 6 carries out glass slide for spraying pure water
It rinses；The drying module 7 is located on locating module 4, and drying module 7 matches the drying for realizing glass slide with locating module 4；
The operation panel 8 is used to input the quantity that dye liquor is added dropwise and interval time and the time of flushing；
This method comprises the following steps：
Step 1：The parameters such as dye number of drops, interval time and the time of flushing are inputted by operation panel 8；
Step 2：The glass slide for being coated with haemocyte is positioned over locating module 4, and locating module 4 clamps glass slide, real
Now position；
Step 3：Staining modules 5 are moved to one 2 top of color solution bottle, and staining modules 5 pick up the dropper in color solution bottle 1；
Step 4：The staining modules 5 that carry dropper are moved to the centre position inside shell 1；
Step 5：Staining modules 5 are drop by drop added drop-wise to smear according to pre-set parameter, by Wright's stain
On；
Step 6：Staining modules 5 repeat step 3-five and the PBS dye liquors in color solution bottle 23 are added drop-wise to painting on piece, stand
30min；
Step 7：Flushing nozzle 6 slowly sprays pure water and is rinsed to smear, washing time 3min；
Step 8：Drying module 7, which blows out natural wind, makes smear rapid airing.
The position of clamp arm (55) clamping dropper in the step 3 is the glass tube section of dropper.
After Wright's stain is added drop-wise to smear in the step 5,1-2min is placed.
The quantity that the Wright's stain is added dropwise is that Wright's stain fully covers on smear surface.
PBS dye liquors are slowly drop by drop added to painting on piece in the step 6, stand 30min.
Time of the smear through pure water rinsing at least 3min in the step 7.
The locating module 4 includes objective table 41, fixture block 42, screw rod 1 and fixed motor 44,41 water of the objective table
Flat to be arranged on stabilizer blade 11, objective table 41 is located at the lower section of shell 1, and symmetrical be horizontally disposed in 41 upper surface of objective table has two sections
Sliding slot；42 quantity of the fixture block is two, and fixture block 42 is located in 41 liang of sliding slots of objective table；The screw rod 1 is set to loading
In the sliding slot of platform 41, screw rod 1 is located at the thread rotary orientation in two sections of sliding slots on the contrary, fixture block 42 is connected with screw rod 1 by screw thread
It connects, fixture block 42 can the horizontal sliding on screw rod 1；The fixed motor 44 is built in objective table 41, fixed motor 44 and screw rod
One 43 are connected.When work, smear is placed on objective table 41, fixed motor 44 drives screw rod 1 to rotate, and fixture block 42 exists
It moves toward one another under the drive of screw rod 1 and is drawn close to centre, smear is clamped, realize the positioning of smear.
The staining modules 5 include screw rod 2 51, sliding block 52, clamping cylinder 53, telescopic cylinder 1, clamp arm 55, stretch
Cylinder 2 56, crushing block 57 and dyeing motor 58, the screw rod 2 51 are horizontally placed on the top of shell 1, and screw rod 2 51 is located at
In shell 1；The sliding block 52 is connected through a screw thread on screw rod 2 51；The clamping cylinder 53 is horizontally arranged at sliding block 52
Lower end；One 54 quantity of the telescopic cylinder is two, and telescopic cylinder 1 is symmetrical to be vertically installed on clamping cylinder 53, folder
Tight cylinder 53 drives telescopic cylinder 1 in the same direction or counter motion；55 quantity of the clamp arm is two, and clamp arm 55 is horizontally fixed on
The bottom end of telescopic cylinder 1, clamp arm 55 is for being clamped dropper；2 56 quantity of the telescopic cylinder is two, 2 56 water of telescopic cylinder
It is flat to be arranged in the lower end of telescopic cylinder 1；The crushing block 57 is fixed on the end of telescopic cylinder 2 56, and crushing block 57 is used for
Squeeze the Capsule part of dropper；The dyeing motor 58 is mounted on shell 1, and dyeing motor 58 is for driving 2 51 turns of screw rod
It is dynamic.Then, dyeing motor 58 drives screw rod 2 51 to rotate, and sliding block 52 is moved to the left to color solution bottle one with the movement of screw rod 2 51
2 top, telescopic cylinder 1 drives clamp arm 55 to move downward and picks up dropper, after dropper removes color solution bottle 1 by clamp arm 55,
Dyeing motor 58 rotates backward, and sliding block 52 is moved to the centre position of shell 1, and dropper synchronizing moving is stretched to 1 centre position of shell
Contracting cylinder moves downward, and makes the sharp mouth of dropper close to smear；Then 2 56 relative motion of telescopic cylinder makes crushing block 57 to dropper
Capsule squeezed, the Wright's stain in dropper is added drop-wise to painting on piece, the quantity and interval that the state modulator of input is added dropwise
Time.
The Flushing nozzle 6 is set on fixture block 42, and Flushing nozzle 6 can be moved with fixture block 42, is realized to nozzle
It adjusts, rinses spraying as fixture block 42 moves, can adapt to different size of smear, guarantee reaches best flushing effect.
The drying module 7 includes rubber bag tank 71, spring 72, holder 73, blowing nozzle 74 and air accumulator, the rubber bag tank 71
It is fixed in the left and right sidewall of shell 1, rubber bag tank 71 is in sustained height with clamping cylinder 53, and rubber bag tank 71 is internally provided with sky
Chamber is provided with air inlet and gas outlet on rubber bag tank 71, and air inlet position has check valve, gas outlet to be communicated with air accumulator；It is described
Spring 72 is located in rubber bag tank 71, and spring 72 thinks that capsule restores to the original state for making；The holder 73 is fixed on fixture block 42；It is described
Blowing nozzle 74 is mounted on holder 73, and angle of the blowing nozzle 74 on holder 73 can be adjusted, and blowing nozzle 74 is connected with air accumulator,
Blowing nozzle 74 is used to spray air to smear.During clamping cylinder 53 moves left and right, clamping cylinder 53 and rubber bag tank 71
It mutually hits, the effect of buffering is played to clamping cylinder 53, while internal air is discharged because of by compression for rubber bag tank 71,
Then it restores to the original state under the action of spring 72, realizes to air accumulator and supply air, the air inside air accumulator passes through air blowing
Mouth 74 sprays, and realizes the rapid airing of smear.
Specific workflow is as follows：
When work, smear is placed on objective table 41, fixed motor 44 drives screw rod 1 to rotate, and fixture block 42 is in screw rod
It moves toward one another under one 43 drive and is drawn close to centre, smear is clamped, realize the positioning of smear；Spraying is rinsed with fixture block 42
Movement, can adapt to different size of smear, and guarantee reaches best flushing effect.
Then, dyeing motor 58 drives screw rod 2 51 to rotate, and sliding block 52 is moved to the left to dye with the movement of screw rod 2 51
The top of liquid bottle 1, telescopic cylinder 1 drive clamp arm 55 to move downward and pick up dropper, and dropper removes color solution bottle by clamp arm 55
After one 2, dyeing motor 58 rotates backward, and sliding block 52 is moved to the centre position of shell 1, among dropper synchronizing moving to shell 1
Position, telescopic cylinder move downward, and make the sharp mouth of dropper close to smear；Then 2 56 relative motion of telescopic cylinder makes crushing block 57
The Capsule of dropper is squeezed, the Wright's stain in dropper is added drop-wise to painting on piece, the quantity of the state modulator dropwise addition of input
And interval time, it repeats the above steps, PBS dye liquors is added drop-wise to painting on piece.
During clamping cylinder 53 moves left and right, clamping cylinder 53 is hit with 71 phase of rubber bag tank, to clamping cylinder 53
The effect of buffering is played, while rubber bag tank 71 by compression and by internal air because be discharged, then in the effect of spring 72
Lower reinstatement realizes to air accumulator and supplies air, and the air inside air accumulator is sprayed by blowing nozzle 74, realizes smear
Rapid airing.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (6)
1. a kind of Medical blood cell dyeing method, it is characterised in that：The colouring method includes the following steps：
Step 1：Dye number of drops, the time parameter of interval time and flushing are inputted in operation panel (8)；
Step 2：The glass slide for being coated with haemocyte is positioned over objective table (41), and fixture block (42) clamps glass slide, and it is fixed to realize
Position；
Step 3：Clamp arm (55) is moved to above color solution bottle one (2), and clamp arm (55) picks up the dropper in color solution bottle one (2)；
Step 4：Clamp arm (55) holds dropper and is moved to the internal centre position of shell (1)；
Step 5：According to pre-set parameter, Wright's stain one is dripped one by the of short duration Capsule for squeezing dropper of crushing block (57)
It is added drop-wise to painting on piece to drop；
Step 6：It repeats step 3 and the PBS dye liquors in color solution bottle two (3) is added drop-wise to painting on piece to step 5；
Step 7：Flushing nozzle (6) slowly sprays pure water and is rinsed to smear, washing time 3min；
Step 8：Blowing nozzle (74) blowout natural wind makes smear rapid airing.
2. a kind of Medical blood cell dyeing method according to claim 1, it is characterised in that：Clamp arm in the step 3
(55) position of clamping dropper is the glass tube section of dropper.
3. a kind of Medical blood cell dyeing method according to claim 1, it is characterised in that：By Rui Shi in the step 5
After dye liquor is added drop-wise to smear, 1-2min is placed.
4. a kind of Medical blood cell dyeing method according to claim 1, it is characterised in that：What the Wright's stain was added dropwise
Quantity is that Wright's stain fully covers on smear surface.
5. a kind of Medical blood cell dyeing method according to claim 1, it is characterised in that：By PBS in the step 6
Dye liquor is slowly drop by drop added to painting on piece, stands 30min.
6. a kind of Medical blood cell dyeing method according to claim 1, it is characterised in that：Smear passes through in the step 7
The time of pure water rinsing at least 3min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810179545.1A CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810179545.1A CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108426757A true CN108426757A (en) | 2018-08-21 |
| CN108426757B CN108426757B (en) | 2020-10-09 |
Family
ID=63157815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810179545.1A Active CN108426757B (en) | 2018-03-05 | 2018-03-05 | Medical blood cell staining method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108426757B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204718U (en) * | 2011-09-22 | 2012-04-25 | 汕头大学医学院第一附属医院 | Slide specimen dyeing machine |
| CN103267668A (en) * | 2013-05-02 | 2013-08-28 | 刘洪莉 | Configurable multifunctional dyeing instrument |
| CN207964417U (en) * | 2018-01-23 | 2018-10-12 | 杭州市余杭区中医院 | A kind of device that can be dyed in batches |
-
2018
- 2018-03-05 CN CN201810179545.1A patent/CN108426757B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204718U (en) * | 2011-09-22 | 2012-04-25 | 汕头大学医学院第一附属医院 | Slide specimen dyeing machine |
| CN103267668A (en) * | 2013-05-02 | 2013-08-28 | 刘洪莉 | Configurable multifunctional dyeing instrument |
| CN207964417U (en) * | 2018-01-23 | 2018-10-12 | 杭州市余杭区中医院 | A kind of device that can be dyed in batches |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
| CN110631882B (en) * | 2019-10-22 | 2022-01-28 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108426757B (en) | 2020-10-09 |
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