CN208898866U - A kind of Promega plasmid extraction reagent extraction mechanism - Google Patents
A kind of Promega plasmid extraction reagent extraction mechanism Download PDFInfo
- Publication number
- CN208898866U CN208898866U CN201821396193.7U CN201821396193U CN208898866U CN 208898866 U CN208898866 U CN 208898866U CN 201821396193 U CN201821396193 U CN 201821396193U CN 208898866 U CN208898866 U CN 208898866U
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- CN
- China
- Prior art keywords
- culture dish
- air
- dish holder
- heater
- holder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 29
- 239000013612 plasmid Substances 0.000 title claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 13
- 230000007246 mechanism Effects 0.000 title claims abstract description 12
- 239000002699 waste material Substances 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 238000002386 leaching Methods 0.000 claims 1
- 238000001035 drying Methods 0.000 abstract description 9
- 239000002274 desiccant Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 description 9
- 238000010586 diagram Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of Promega plasmid extraction reagent extraction mechanisms, including outer cabinet, cabinet door is provided on the outer cabinet, and cabinet door is rotatablely connected by hinge and outer cabinet, the outer cabinet is internally provided with air-heater, the side of the air-heater is provided with culture dish holder, the side of the culture dish holder is provided with air inlet pipe, the other side of the culture dish holder is provided with escape pipe, valve is mounted in the escape pipe and air inlet pipe, side door is installed on the culture dish holder, the utility model is provided with culture dish holder and air-heater, the air-heater of setting will blow hot wind into culture dish holder by air inlet pipe after operation, to which the moisture drying on moist culture dish surface later will be cleaned, after completing drying, culture dish is stored by the desiccant placed in the hollow cylinder of setting Environment continued drying out, thus be conducive to culture dish storage during keep drying.
Description
Technical field
The utility model belongs to plasmid extraction technical field, and in particular to a kind of Promega plasmid extraction reagent extractor
Structure.
Background technique
Plasmid extraction method removes RNA, and plasmid and bacterial genomes DNA are separated, isolating protein and other impurity are removed,
To obtain relatively pure plasmid, there are many kinds of the methods for extracting Plasmid DNA, point can be divided into from extraction yield Trace bio-element,
Middle amount is extracted, is largely extracted, and is extracted from using to divide to be divided into generally extracting on instrument with kit method, from concrete operation method
Alkaline lysis, boiling method, toothpick method etc. can be divided by dividing, and a variety of different methods respectively have its advantage and disadvantage, according to different experiments
Purpose can use suitable extracting method, and alkaline lysis is suitable for the extraction of mini-scale plasmid DNA.
But plasmid extraction reagent extraction mechanism using effect currently on the market is bad, it is inconvenient to take culture dish, and
The culture dish moisture-sensitive of storage and interfere the later period to use, when carrying out ice bath immersion, centrifuge tube easily floats, and is unfavorable for the complete of solution
Complete immersion
Utility model content
The purpose of this utility model is to provide a kind of Promega plasmid extraction reagent extraction mechanisms, to solve above-mentioned back
The culture dish moisture-sensitive of storage is proposed in scape technology and the later period is interfered to use, and when carrying out ice bath dip operation, centrifuge tube is easily floating
It rises, is unfavorable for the problem of solution is completely soaked.
To achieve the above object, the utility model provides the following technical solutions: a kind of Promega plasmid extraction reagent extraction
Mechanism, including outer cabinet are provided with cabinet door on the outer cabinet, and cabinet door is rotatablely connected by hinge and outer cabinet, the outer cabinet it is interior
Portion is provided with air-heater, and the side of the air-heater is provided with culture dish holder, the side setting of the culture dish holder
There is air inlet pipe, the other side of the culture dish holder is provided with escape pipe, is mounted on valve in the escape pipe and air inlet pipe
, side door is installed, the side door is rotatablely connected by hinge and culture dish holder, the training on the culture dish holder
That supports ware holder is internally provided with plate, hollow cylinder is equipped with below the plate, the top of the hollow cylinder is provided with
To connect cylinder, the hollow cylinder screws connect cylinder with plate by screw thread to be connect screw thread, and outer sleeve, institute are equipped on the hollow cylinder
It states and is provided with culture dish rack below outer sleeve, be provided with round bar below the culture dish holder, on the round bar
Sunpender is installed, sliding shoe is arranged on the sunpender, bolt, the side setting of the sliding shoe are provided on the sliding shoe
There is circle frame, the circle frame is fixedly connected by bracket with sliding shoe, and pressure ram, the sunpender are equipped on the circle frame
Lower section be provided with fermentation vat, waste collection pond, the row of being equipped in the waste collection pond are provided with below the fermentation vat
Water pipe, the fermentation vat are connected with waste collection pond by drainpipe, and the side of the waste collection pond is provided with small-sized ice
Cabinet, the small-sized refrigerator-freezer and air-heater are electrically connected with external power supply.
Preferably, hole is opened up on the outer sleeve.
Preferably, hole is opened up on the hollow cylinder.
Preferably, threaded hole is offered on the sunpender.
Preferably, the threaded hole to match with pressure ram is provided on the circle frame.
The utility model compared with prior art, the utility model has the beneficial effects that
(1) the utility model is provided with culture dish holder and air-heater, and the air-heater of setting will pass through after operation
Air inlet pipe blows hot wind into culture dish holder, so that the moisture of cleaning later moist culture dish surface be dried, completes to dry
It after dry, is continued drying out by the environment that the desiccant placed in the hollow cylinder of setting stores culture dish, to be conducive to training
Drying is kept during supporting ware storage.
(2) the utility model is provided with fermentation vat and circle frame, and centrifuge tube is placed on the inside of circle frame and passes through extruding
Bar carries out extruding fixation, so that when carrying out ice bath operation centrifuge tube be avoided easily to float asking of causing centrifuge tube not to be completely soaked
Topic, meanwhile, it is not necessarily to manual Continued depression centrifuge tube, it is more convenient.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model;
Fig. 2 is the outside drawing of the outer cabinet of the utility model;
Fig. 3 is the structural schematic diagram of the utility model culture dish holder;
Fig. 4 is the outside drawing of the utility model hollow cylinder;
Fig. 5 is the partial sectional view of the utility model hollow cylinder;
Fig. 6 is the structural schematic diagram of the utility model circle frame;
Fig. 7 is the enlarged drawing at the utility model A;
In figure: 1- outer cabinet, 2- cabinet door, 3- air-heater, 4- air inlet pipe, 5- side door, 6- culture dish holder, 7- valve, 8-
Escape pipe, 9- round bar, 10- fermentation vat, the small-sized refrigerator-freezer of 11-, 12- waste collection pond, 13- drainpipe, 14- plate, 15- are hollow
Cylinder, 16- outer sleeve, 17- culture dish rack, 18- screw thread hang connect cylinder, 19- circle frame, 20- pressure ram, 21- bolt, 22-
Bar, 23- sliding shoe.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
It please refers to shown in Fig. 1-Fig. 7, the utility model provides a kind of technical solution: a kind of Promega plasmid extraction reagent
Extraction mechanism, including outer cabinet 1 are provided with cabinet door 2 on outer cabinet 1, and cabinet door 2 is rotatablely connected by hinge and outer cabinet 1, outer cabinet 1
It is internally provided with air-heater 3, air-heater 3 will generate hot wind after being powered, to easily culture dish be dried, air-heater 3
Side be provided with culture dish holder 6, the side of culture dish holder 6 is provided with air inlet pipe 4, culture dish holder 6 it is another
Side is provided with escape pipe 8, and valve 7 is mounted on escape pipe 8 and air inlet pipe 4, side door 5 is equipped on culture dish holder 6,
Side door 5 is rotatablely connected by hinge and culture dish holder 6, and culture dish holder 6 is internally provided with plate 14, plate 14
Lower section is equipped with hollow cylinder 15, and hollow cylinder 15 is internally provided with desiccant, can be in air by the desiccant of setting
Moisture is dried, and the top of hollow cylinder 15 is provided with screw thread to connect cylinder 18, and hollow cylinder 15 is by screw thread to connect cylinder 18 and plate
14 screw connection, and outer sleeve 16 is equipped on hollow cylinder 15, and the lower section of outer sleeve 16 is provided with culture dish rack 17, culture dish
The lower section of holder 6 is provided with round bar 9, and sunpender 22 is equipped on round bar 9, and sliding shoe 23, sliding shoe 23 are arranged on sunpender 22
On be provided with bolt 21, the side of sliding shoe 23 is provided with circle frame 19, and circle frame 19 connects by the way that bracket and sliding shoe 23 are fixed
It connects, pressure ram 20 is installed on circle frame 19, the lower section of sunpender 22 is provided with fermentation vat 10, and the lower section of fermentation vat 10 is provided with useless
Liquid collecting pit 12 is equipped with drainpipe 13 in waste collection pond 12, there is the sealing valve that can be retained, fermentation vat 10 on drainpipe 13
It is connected with waste collection pond 12 by drainpipe 13, the side of waste collection pond 12 is provided with small-sized refrigerator-freezer 11, small-sized refrigerator-freezer
Ice cube is placed in 11, small-sized refrigerator-freezer 11 and air-heater 3 are electrically connected with external power supply.
Enter for the ease of external air, in the present embodiment, it is preferred that open up hole on outer sleeve 16.
The air entered for the ease of outside is contacted with desiccant, in the present embodiment, it is preferred that offer on hollow cylinder 15
Hole.
Fixed position on sunpender 22 is adjusted for the ease of sliding shoe 23, in the present embodiment, it is preferred that open on sunpender 22
Equipped with threaded hole.
It is rotated for the ease of pressure ram 20, in the present embodiment, it is preferred that be provided on circle frame 19 and 20 phase of pressure ram
The threaded hole matched.
The working principle and process for using of the utility model: in use, needing to use culture dish to prepare needed for experiment
Culture medium, the culture dish used are placed in culture dish holder 6 after cleaning, can conveniently take culture dish and
It is in culture dish in the storage environment of drying, after powering on, air-heater 3 will be blown into hot wind into culture dish holder 6,
To which the moist culture dish just cleaned on culture dish rack 17 drying will be placed on, outer sleeve 16 is rotated after the completion of drying,
The hole aligning for making the hole opened up on outer sleeve 16 and being opened up on hollow cylinder 15, to enable external air and hollow cylinder
The desiccant placed in 15 is contacted, so that the culture dish placed in culture dish holder 6 be made to be continuously in dry environment
In, needing to carry out ice bath to the centrifuge tube for filling solution during extracting experimental implementation will be small when carrying out ice bath operation
Ice cube taking-up in type refrigerator-freezer 11 is put into fermentation vat 10, is added clear water into small-sized refrigerator-freezer 11, will be needed to carry out ice bath
Centrifuge tube is placed on 19 inside of circle frame, and is squeezed by rotating pressure ram 20 centrifuge tube, and centrifuge tube is consolidated in completion
After fixed, the height fixed on sunpender 22 of sliding shoe 23 is adjusted according to the height of water, so that fixed centrifuge tube be made to be at
Ice bath is carried out in ice water.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of Promega plasmid extraction reagent extraction mechanism, including outer cabinet (1), it is characterised in that: set on the outer cabinet (1)
It is equipped with cabinet door (2), and cabinet door (2) is rotatablely connected by hinge and outer cabinet (1), the outer cabinet (1) is internally provided with air-heater
(3), the side of the air-heater (3) is provided with culture dish holder (6), and the side of the culture dish holder (6) is provided with
The other side of air inlet pipe (4), the culture dish holder (6) is provided with escape pipe (8), the escape pipe (8) and air inlet pipe (4)
On be mounted on valve (7), be equipped with side door (5) on the culture dish holder (6), the side door (5) by hinge and training
Ware holder (6) rotation connection is supported, the culture dish holder (6) is internally provided with plate (14), the plate (14)
Lower section is equipped with hollow cylinder (15), and the top of the hollow cylinder (15) is provided with screw thread to connect cylinder (18), the hollow cylinder (15)
Connect cylinder (18) is screwed with plate (14) by screw thread and is connect, is equipped with outer sleeve (16) on the hollow cylinder (15), it is described outer
It is provided with below sleeve (16) culture dish rack (17), is provided with round bar (9) below the culture dish holder (6),
It is equipped with sunpender (22) on the round bar (9), is arranged with sliding shoe (23) on the sunpender (22), set on the sliding shoe (23)
It is equipped with bolt (21), the side of the sliding shoe (23) is provided with circle frame (19), and the circle frame (19) passes through bracket and cunning
Motion block (23) is fixedly connected, and is equipped with pressure ram (20) on the circle frame (19), is provided with leaching below the sunpender (22)
It steeps pond (10), is provided with waste collection pond (12) below the fermentation vat (10), is equipped on the waste collection pond (12)
Drainpipe (13), the fermentation vat (10) are connected with waste collection pond (12) by drainpipe (13), the waste collection pond
(12) side is provided with small-sized refrigerator-freezer (11), and the small-sized refrigerator-freezer (11) and air-heater (3) are electrically connected with external power supply.
2. a kind of Promega plasmid extraction reagent extraction mechanism according to claim 1, it is characterised in that: the housing
Hole is opened up on cylinder (16).
3. a kind of Promega plasmid extraction reagent extraction mechanism according to claim 1, it is characterised in that: described hollow
Hole is opened up on cylinder (15).
4. a kind of Promega plasmid extraction reagent extraction mechanism according to claim 1, it is characterised in that: the sunpender
(22) threaded hole is offered on.
5. a kind of Promega plasmid extraction reagent extraction mechanism according to claim 1, it is characterised in that: the circle
The threaded hole to match with pressure ram (20) is provided on frame (19).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821396193.7U CN208898866U (en) | 2018-08-28 | 2018-08-28 | A kind of Promega plasmid extraction reagent extraction mechanism |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821396193.7U CN208898866U (en) | 2018-08-28 | 2018-08-28 | A kind of Promega plasmid extraction reagent extraction mechanism |
Publications (1)
Publication Number | Publication Date |
---|---|
CN208898866U true CN208898866U (en) | 2019-05-24 |
Family
ID=66570602
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201821396193.7U Expired - Fee Related CN208898866U (en) | 2018-08-28 | 2018-08-28 | A kind of Promega plasmid extraction reagent extraction mechanism |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN208898866U (en) |
-
2018
- 2018-08-28 CN CN201821396193.7U patent/CN208898866U/en not_active Expired - Fee Related
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190524 |