CN117405803B - Cell detection device and method based on paper-based discharge technology - Google Patents
Cell detection device and method based on paper-based discharge technology Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 56
- 238000005516 engineering process Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims description 16
- 239000007788 liquid Substances 0.000 claims abstract description 115
- 238000000605 extraction Methods 0.000 claims abstract description 44
- 238000007789 sealing Methods 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims description 7
- 150000002500 ions Chemical class 0.000 claims description 7
- 238000012544 monitoring process Methods 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 239000004020 conductor Substances 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 230000006978 adaptation Effects 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
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- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
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Abstract
The invention discloses a cell detection device and a cell detection method based on a paper-based discharge technology, which relate to the technical field of cell detection devices, and the cell detection device based on the paper-based discharge technology comprises a base bracket and further comprises: the first conveying belt is rotatably arranged on the base bracket, a plurality of clamping hoops are arranged on the circumference of the first conveying belt, and the clamping hoops are used for clamping the capillary tube; the extraction liquid storage box is arranged below the first conveying belt and is used for enabling a first port on the capillary to face the extraction liquid storage box when the first conveying belt drives the capillary to overturn to the lower side; according to the invention, the extraction liquid, the dehydrated cells and the gas are injected into the plurality of capillaries on the first conveyor belt in batches and batches by virtue of the rotation of the first conveyor belt, so that manual operation is not needed, the detection efficiency is further improved, and compared with the cell detection method in the prior art, the device can effectively improve the batch of cell detection.
Description
Technical Field
The invention belongs to the technical field of cell detection devices, and particularly relates to a cell detection device based on a paper-based discharge technology.
Background
Single cell technology has been developed rapidly in recent years, and along with the continuous development of single cell sequencing, single cell transcriptome and other technologies, human beings have thoroughly understood cell population heterogeneity from the level of DNA and RNA, and single cell analysis has important significance for researching intercellular heterogeneity; mass spectrometers are widely used in single cell analysis due to their high sensitivity, accurate structural identification, quantitative analysis, and other characteristics. However, single cells only have tiny volumes of fL-pL, if the volume of the extraction liquid is too large, the concentration of the metabolite in the cells is too low due to excessive dilution, and the detection range of mass spectrum is exceeded; if the dilution is too small, the amount of the sample available for analysis is too small, and the control difficulty of the sample is greatly increased;
the existing researchers use paper spray mass spectrometry technology for cell analysis, the method takes a triangular paper base as a carrier, a sample is added on the surface of the paper base, high voltage is applied, a solvent is driven by the voltage to dissolve and extract a target in the sample, so that the target is migrated and ionized, and electrospray is formed at the tip of the paper base to enter the mass spectrum for analysis; the method is convenient to operate and does not cause excessive dilution.
In the prior art, grant bulletin number: CN112444583a, authorized bulletin day: 2021.03.05 a cell detection device and method based on paper-based discharge technology, the cell detection device comprising a mass spectrometer; the paper has a tip, and the electrical conductor is connected to the paper and is adapted to be connected to a power source; the bearing piece is provided with a plurality of bearing positions, and the bearing positions are used for bearing cells; the end part of the fluid pipeline is matched with the bearing position, a space isolated from the outside is formed between the bearing position and the fluid pipeline, and cells of the bearing position are positioned in the space; the fluid pipeline is provided with an inlet and an outlet which are respectively communicated with the space; the gas source is used for supplying gas into the space, driving substances in the space into the fluid pipeline, and spraying substances discharged from the outlet of the fluid pipeline onto paper between the tip and the conductor. The invention has the advantages of accurate detection, automation and the like;
in the above patent, although high-quality cell detection can be achieved, the detection efficiency is reduced when a plurality of experiments are required, and therefore we propose a cell detection device based on paper-based discharge technology.
Disclosure of Invention
The technical problem underlying the present invention is to overcome the disadvantages of the prior art by providing a cell detection device based on paper-based discharge technology which overcomes or at least partially solves the above-mentioned problems.
In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that: cell detection device based on paper-based discharge technique, including the base support, still include: the first conveying belt is rotatably arranged on the base bracket, a plurality of clamping hoops are arranged on the circumference of the first conveying belt, and the clamping hoops are used for clamping the capillary tube; the extraction liquid storage box is arranged below the first conveying belt and is used for enabling extraction liquid in the extraction liquid storage box to enter the capillary through the first port when the first conveying belt drives the capillary to overturn to the lower side and the first port on the capillary faces the extraction liquid storage box; a liquid inlet zone for discharging dehydrated cells into the capillary when the capillary reaches the liquid inlet zone; and the gas source supply area is used for discharging gas into the capillary when the capillary reaches the gas source supply area, so that the extraction liquid and the cell extraction substance of the dehydrated cell product are sprayed onto the paper from the first port, and ions are ejected from the tip of the paper after ionization of the paper and enter the mass spectrometer from the sample inlet of the mass spectrometer for detection.
Preferably, a sleeve is fixedly connected to the capillary tube in a sleeved mode, a second port of the capillary tube is located in the sleeve, a connecting rod is fixedly connected to the clamping hoop, a sealing cover is fixedly connected to the connecting rod, the sleeve is inserted into the sealing cover, a connecting pipe is fixedly connected to the sleeve, one end of the connecting pipe is led into the capillary tube, and the connecting pipe is used for discharging dehydrated cells or supplying gas into the capillary tube through the connecting pipe.
Preferably, the liquid inlet area and the air source supply area respectively comprise a liquid supply pipe and an air supply pipe, the base support is respectively and fixedly connected with a connecting plate corresponding to the liquid supply pipe and the air supply pipe, and a position sensor is arranged on the connecting plate and used for monitoring the position of the capillary tube and discharging dehydrated cells or supplied air into the connecting pipe when the connecting pipe on the capillary tube corresponds to the liquid supply pipe or the air supply pipe.
Further, fixedly connected with slide bar on the connecting plate, sliding connection has the installation piece on the slide bar, the installation piece is connected through the extension spring with slide bar one end, liquid supply pipe, air supply pipe are installed respectively on corresponding installation piece, all install the magnet of mutual absorption on one end of liquid supply pipe, air supply pipe, connecting pipe.
Further, the method further comprises the following steps: the first rack is positioned on the left side of the first conveying belt; the circumference fixedly connected with and centre gripping hoop is corresponding connecting block on the first conveyer belt, rotate on the connecting block and be connected with the pivot, centre gripping hoop links to each other with the pivot is fixed, fixedly connected with gear in the pivot, the gear meshes with first rack mutually, is used for with reentrant feed liquor district when the capillary upset makes first port upwards.
Further, the method further comprises the following steps: the second rack is positioned on the right side of the liquid inlet area, and a liquid control area is arranged between the liquid inlet area and the second rack and used for preventing the extraction liquid in the first port of the capillary tube from flowing out; the second rack is used for overturning the capillary again so that the first port is downward.
Preferably, the closing cover is provided with a slot and a liquid storage tank, the sleeve corresponds to the slot, and the second port at one end of the capillary tube corresponds to the liquid storage tank.
Further, the clamping hoops are symmetrically arranged on two sides of the first conveying belt.
Further, fixedly connected with goes up the mounting bracket on the base support, it is provided with the second conveyer belt to go up the rotation of mounting bracket, fixedly connected with location tool on the outer wall of second conveyer belt, the opening has been seted up to one side of location tool, location tool is used for right the paper is fixed a position and is placed.
The application method of the cell detection device based on the paper-based discharge technology mainly comprises the following steps:
s1, installing a capillary on a clamping hoop, driving a plurality of clamping hoops on a first conveyor belt to rotate through rotation of the first conveyor belt, and driving the capillary to move towards the lower side of the first conveyor belt after the rotation of the first conveyor belt, so that a first port is downward and enters an extract liquid storage box, and an extract liquid in the extract liquid storage box enters the capillary;
s2, when the capillary tube with the extract liquid moves to a liquid inlet area, the liquid inlet area discharges dehydrated cells into the capillary tube;
s3, when the capillary tube with the extraction liquid and the dehydrated cells moves to the air source supply area, air is supplied to the capillary tube through the connecting pipe, the dehydrated cells are extracted by the extraction liquid upwards by the air entering the capillary tube, the extracted cell extraction product is sprayed out onto the paper from the first port of the capillary tube, the conductive clamp connected with the paper discharges, the cell extraction product is ionized, ions are sprayed out from the tip of the paper and enter the sample inlet of the mass spectrometer for detection, and then the cell detection is completed.
After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects: according to the invention, the extraction liquid, the dehydrated cells and the gas are injected into the plurality of capillaries on the first conveyor belt in batches and batches by virtue of the rotation of the first conveyor belt, so that manual operation is not needed, the detection efficiency is further improved, and compared with the cell detection method in the prior art, the device can effectively improve the batch of cell detection.
Drawings
In the drawings:
FIG. 1 is a schematic diagram of a cell detection device based on paper-based discharge technology according to the present invention;
FIG. 2 is a schematic diagram of the structure of the cell detection device according to FIG. 1A based on the paper-based discharge technique;
FIG. 3 is a schematic diagram showing a three-dimensional structure of a cell detection device based on a paper-based discharge technique according to the present invention;
FIG. 4 is a schematic diagram of the structure of the cell detection device according to FIG. 3B based on the paper-based discharge technique;
FIG. 5 is a schematic diagram of a capillary tube of a cell detection device based on a paper-based discharge technique according to the present invention;
FIG. 6 is a schematic diagram of the structure of a first rack and a second rack of a cell detection device based on paper-based discharge technology according to the present invention;
FIG. 7 is a schematic diagram of the structure of the upper mounting frame of the cell detection device based on the paper-based discharge technology;
FIG. 8 is a schematic diagram of the structure of a first rack and a second rack of a cell detection device based on paper-based discharge technology according to the present invention;
fig. 9 is a right side view of a cell detection device based on a paper-based discharge technique according to the present invention.
In the figure: 1. a base bracket; 11. placing a plate; 12. a first conveyor belt; 121. a connecting shaft; 13. a connecting block; 131. a rotating shaft; 132. a gear; 134. a clamping hoop; 135. a connecting rod; 14. a capillary tube; 140. a first port; 1401. a second port; 141. a sleeve; 142. a closing cap; 143. a slot; 144. a liquid storage tank; 145. a connecting pipe; 15. a connecting plate; 150. a liquid supply pipe; 151. a mounting block; 152. a locking band; 153. an air supply pipe; 154. a slide bar; 155. a tension spring; 156. a position sensor; 16. a motor; 17. a first rack; 171. a fixing frame; 18. a second rack; 2. an upper mounting frame; 21. a second conveyor belt; 22. positioning jig; 23. an opening; 3. a liquid inlet area; 4. a liquid control area; 5. an air source supply region; 6. an extract liquid storage box; 7. a discharge spray zone; 8. a mass spectrometer; 81. and a sample inlet.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
Example 1:
referring to fig. 1 to 9, a cell detection device based on paper-based discharge technology includes a base support 1, a placement plate 11 is installed on the base support 1, and can be used for placing an extract liquid storage box 6, and further includes: a first conveyor belt 12 rotatably disposed on the base frame 1, wherein a plurality of clamping hoops 134 are circumferentially disposed on the first conveyor belt 12, and the clamping hoops 134 are used for clamping the capillary 14; the extraction liquid storage box 6 is arranged below the first conveying belt 12, and is used for enabling the extraction liquid in the extraction liquid storage box 6 to enter the capillary tube 14 through the first port 140 when the first conveying belt 12 drives the capillary tube 14 to overturn downwards so that the first port 140 on the capillary tube 14 faces the extraction liquid storage box 6; a liquid inlet zone 3 for discharging dehydrated cells into the capillary 14 when the capillary 14 reaches the liquid inlet zone 3; the gas source supply area 5 is used for exhausting gas into the capillary 14 when the capillary 14 reaches the gas source supply area 5, so that the extraction liquid and the cell extraction substance of the dehydrated cell product are sprayed onto the paper from the first port 140, and ions are emitted from the tip of the paper after ionization of the paper and enter the mass spectrometer 8 from the sample inlet 81 of the mass spectrometer 8 for detection; the capillary tube 14 is sleeved with a sleeve 141 fixedly connected with a second port 1401 of the capillary tube 14, the second port 1401 of the capillary tube 14 is positioned in the sleeve 141, the clamping hoop 134 is fixedly connected with a connecting rod 135, the connecting rod 135 is fixedly connected with a sealing cover 142, the sleeve 141 is inserted into the sealing cover 142, the sleeve 141 is fixedly connected with a connecting pipe 145, one end of the connecting pipe 145 is led into the capillary tube 14, and the connecting pipe 145 is used for discharging dehydrated cells or supplying gas into the capillary tube 14 through the connecting pipe 145;
when the device is used, the capillary 14 is mounted on the clamping hoop 134, and it is to be understood that the capillary 14 is provided with a limiting block, and when the capillary 14 is positioned in the clamping hoop 134, the limiting block is positioned in an opening groove of the clamping hoop 134 so as to limit the angle of the capillary 14;
then, by starting the motor 16 arranged on the base bracket 1, the motor 16 drives the connecting shaft 121 connected with the output end of the motor 16 to rotate, the connecting shaft 121 is symmetrically and rotatably connected to the base bracket 1, the first conveyer belt 12 is connected to the connecting shaft 121, so that the motor 16 drives the first conveyer belt 12 to rotate, and when the first conveyer belt 12 rotates, the plurality of clamped capillaries 14 on the first conveyer belt 12 are synchronously driven, and the number of single-side clamping hoops 134 of the first conveyer belt 12 in the device is four;
the first port 140 of the capillary 14 on the upper side of the first conveyer belt 12 is upward, when the first conveyer belt 12 rotates, the capillary 14 is driven to move to the lower side of the first conveyer belt 12, so that the first port 140 is downward and enters the extract liquid storage box 6, the extract liquid in the extract liquid storage box 6 enters the capillary 14, and under the continuous rotation of the first conveyer belt 12, the capillary 14 with the extract liquid is brought to the liquid inlet area 3, and the liquid inlet area 3 discharges dehydrated cells into the capillary 14 through the connecting pipe 145;
continuing to rotate under the continuous rotation of the first conveyer belt 12, the capillary 14 with the extract liquid and dehydrated cells moves to the upper side of the first conveyer belt 12 and enters the air supply region 5, air is supplied to the capillary 14 through the connecting pipe 145, the air entering the capillary 14 can enable the dehydrated cells to be extracted upwards by the extract liquid, the extracted cell extract product is sprayed onto paper from the first port 140 of the capillary 14, the conductive clamp connected with the paper discharges, the cell extract product is ionized, ions are sprayed out from the tip of the paper and enter the sample inlet 81 of the mass spectrometer 8 for detection;
the device injects the extraction liquid, dehydrated cells and gas in batches and batches into the capillaries 14 on the first conveyer belt 12 through the rotation of the first conveyer belt 12, so that manual operation is not needed, and the detection efficiency is improved.
In one embodiment, the liquid inlet area 3 and the air source supply area 5 respectively comprise a liquid supply pipe 150 and an air supply pipe 153, the base bracket 1 is fixedly connected with a connecting plate 15 corresponding to the liquid supply pipe 150 and the air supply pipe 153 respectively, the connecting plate 15 is provided with a position sensor 156 for monitoring the position of the capillary tube 14, and when the connecting pipe 145 on the capillary tube 14 corresponds to the liquid supply pipe 150 or the air supply pipe 153, dehydrated cells or supplied air are discharged into the connecting pipe 145;
one end of the liquid supply pipe 150 is connected with a liquid pump, and the liquid pump is used for pumping dehydrated cells in the container;
one end of the gas supply pipe 153 is connected to a gas pump for sucking gas to be discharged from the gas supply pipe 153;
when the capillary tube 14 is moved to the position sensor 156 of the connection plate 15, dehydrated cells or gas is discharged into the connection tube 145 through the liquid supply tube 150 or the gas supply tube 153 by activating the liquid suction pump or the gas pump.
In one embodiment, a sliding rod 154 is fixedly connected to the connecting plate 15, a mounting block 151 is slidably connected to the sliding rod 154, one end of the mounting block 151 is connected to one end of the sliding rod 154 through a tension spring 155, the liquid supply pipe 150 and the air supply pipe 153 are respectively mounted on the corresponding mounting blocks 151 through locking hoops 152, and magnets which are mutually adsorbed are respectively mounted on one ends of the liquid supply pipe 150, the air supply pipe 153 and the connecting pipe 145;
when the connecting pipe 145 is opposite to the liquid supply pipe 150 or the air supply pipe 153, the magnets on the connecting pipe 145, the liquid supply pipe 150 and the air supply pipe 153 are adsorbed, so that the connecting pipe 145 is connected with the liquid supply pipe 150 and the air supply pipe 153, and the connecting pipe is connected with the tension spring 155 through the mounting block 151, when the capillary 14 moves along with the first conveying belt 12, the liquid supply pipe 150 and the air supply pipe 153 can be synchronously moved, and when the mounting block 151 reaches one end of the connecting plate 15, the blocking effect is separated from the connecting pipe 145.
In one embodiment, further comprising: a first rack 17 positioned on the left side of the first conveyor belt 12; the circumference of the first conveyer belt 12 is fixedly connected with a connecting block 13 corresponding to the clamping hoop 134, the connecting block 13 is rotationally connected with a rotating shaft 131, the clamping hoop 134 is fixedly connected with the rotating shaft 131, the rotating shaft 131 is fixedly connected with a gear 132, and the gear 132 is meshed with the first rack 17 and used for overturning the capillary 14 to enable the first port 140 to upwards enter the liquid inlet area 3; further comprises: the second rack 18 is positioned on the right side of the liquid inlet area 3, and a liquid control area 4 is arranged between the liquid inlet area 3 and the second rack 18 and is used for preventing the extraction liquid in the first port 140 of the capillary tube 14 from flowing out; the second rack 18 is used to turn the capillary 14 over again with the first port 140 down;
the first rack 17 and the second rack 18 are arranged through the fixing frame 171, the fixing frame 171 is fixedly connected with the base bracket 1, and the part of the fixing frame 171 extending towards the first conveying belt 12 is positioned above the first rack 17, so that collision between the capillary 14 and the fixing frame 171 can be avoided;
taking the perspective of fig. 6 as an example, when the capillary 14 moves to the lower left side of the first conveyor belt 12, the gear 132 engages with the first rack 17, causing the gear 132 to rotate 180 degrees such that the first port 140 of the capillary 14 is up and into the intake zone 3;
the first port 140 of the capillary tube 14 is upwards, so that the extraction liquid in the first port 140 can be prevented from flowing out;
when the liquid inlet area 3 is discharged into the capillary 14 to dewater cells, the capillary 14 is rotated again by 180 degrees through the second rack 18, so that the first port 140 is downward, on one hand, the extraction liquid in the capillary 14 can be conveniently mixed with the dewater cells, and on the other hand, when the capillary 14 moves to the upper right side of the first conveyer belt 12 through the first conveyer belt 12, the first port 140 is upward, and the cell extraction substances can be conveniently sprayed out;
it should be appreciated that the number of teeth on the first rack 17 and the second rack 18 can rotate the gear 132 180 degrees.
In one embodiment, the closing cover 142 is provided with a slot 143 and a liquid storage groove 144, the sleeve 141 corresponds to the slot 143, and the second port 1401 at one end of the capillary tube 14 corresponds to the liquid storage groove 144;
the sleeve 141 is inserted into the slot 143 to improve the sealing property to one end of the capillary tube 14, and the reservoir 144 is capable of temporarily storing dehydrated cells discharged.
In one embodiment, the clamping hoops 134 are symmetrically disposed on both sides of the first conveyor belt 12, which can increase the number of capillaries 14 on the first conveyor belt 12, further increasing the detection efficiency.
In one embodiment, an upper mounting frame 2 is fixedly connected to the base bracket 1, a second conveying belt 21 is rotatably arranged on the upper mounting frame 2, a positioning jig 22 is fixedly connected to the outer wall of the second conveying belt 21, an opening 23 is formed in one side of the positioning jig 22, the positioning jig 22 is used for positioning and placing paper, the tip of the paper faces the opening 23, and the paper is conveniently sprayed into the sample inlet 81;
the second conveyor belt 21 is controlled to rotate by a servo motor, so that one end of the capillary 14 is conveniently aligned with paper in the positioning jig 22 to be ejected, and when the paper moves into the area of the discharge ejection area 7, discharge ejection is performed.
Example 2:
referring to fig. 1 to 9, a method for using a paper-based discharge technology-based cell detection device mainly includes the following steps:
s1, mounting a capillary 14 on a clamping hoop 134, driving a plurality of clamping hoops 134 on a first conveyor belt 12 to rotate through rotation of the first conveyor belt 12, and driving the capillary 14 to move towards the lower side of the first conveyor belt 12 after the rotation of the first conveyor belt 12, so that a first port 140 is downward and enters an extract liquid storage box 6, and the extract liquid in the extract liquid storage box 6 enters the capillary 14;
s2, when the capillary 14 with the extract liquid moves to the liquid inlet area 3, the liquid inlet area 3 discharges dehydrated cells into the capillary 14;
s3, when the capillary tube 14 with the extraction liquid and the dehydrated cells moves to the air source supply area 5, air is supplied to the capillary tube 14 through the connecting pipe 145, the air entering the capillary tube 14 enables the dehydrated cells to be extracted by the extraction liquid upwards, the extracted cell extraction product is sprayed onto paper from the first port 140 of the capillary tube 14, the conductive clamp connected with the paper discharges, the cell extraction product is ionized, ions are sprayed out from the tip of the paper and enter the sample inlet 81 of the mass spectrometer 8 for detection, and then cell detection is completed.
According to the invention, the extraction liquid, the dehydrated cells and the gas are injected into the plurality of capillaries 14 on the first conveyer belt 12 in batches by the rotation of the first conveyer belt 12 without manual operation, so that the detection efficiency is improved, and compared with the cell detection method in the prior art, the device can effectively improve the batch of cell detection.
The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any and all simple modifications, equivalent variations and adaptations of the embodiments described above, which are within the scope of the invention, may be made by those skilled in the art without departing from the scope of the invention.
Claims (5)
1. The cell detection device based on the paper-based discharge technology comprises a base bracket (1), and is characterized in that,
further comprises: the first conveying belt (12) is rotatably arranged on the base bracket (1), a plurality of clamping hoops (134) are circumferentially arranged on the first conveying belt (12), and the clamping hoops (134) are used for clamping the capillary tube (14);
the extraction liquid storage box (6) is arranged below the first conveying belt (12) and is used for enabling extraction liquid in the extraction liquid storage box (6) to enter the capillary tube (14) through the first port (140) when the first conveying belt (12) drives the capillary tube (14) to overturn downwards and enable the first port (140) on the capillary tube (14) to face the extraction liquid storage box (6);
a liquid inlet zone (3) for discharging dehydrated cells into the capillary (14) when the capillary (14) reaches the liquid inlet zone (3);
a gas supply zone (5) for exhausting gas into the capillary (14) when the capillary (14) reaches the gas supply zone (5), causing the extract and cell extract of dehydrated cells to be ejected from the first port (140) onto paper and through ionization of the paper, ions to be ejected from the tip of the paper, from a sample inlet (81) of a mass spectrometer (8) into the mass spectrometer (8) for detection;
the capillary tube (14) is sleeved with a sleeve (141) fixedly connected with the capillary tube (14), a second port (1401) of the capillary tube (14) is positioned in the sleeve (141), a connecting rod (135) is fixedly connected with the clamping hoop (134), a sealing cover (142) is fixedly connected with the connecting rod (135), the sleeve (141) is inserted into the sealing cover (142), a connecting pipe (145) is fixedly connected with the sleeve (141), and one end of the connecting pipe (145) is led into the capillary tube (14) so as to discharge dehydrated cells or supply gas into the capillary tube (14) through the connecting pipe (145);
the liquid inlet area (3) and the air source supply area (5) respectively comprise a liquid supply pipe (150) and an air supply pipe (153), the base support (1) is fixedly connected with connecting plates (15) corresponding to the liquid supply pipe (150) and the air supply pipe (153) respectively, the connecting plates (15) are provided with position sensors (156) for monitoring positions of the capillaries (14) so as to discharge dehydrated cells or supply air into the connecting pipes (145) when the connecting pipes (145) on the capillaries (14) correspond to the liquid supply pipe (150) or the air supply pipe (153);
a sliding rod (154) is fixedly connected to the connecting plate (15), a mounting block (151) is connected to the sliding rod (154) in a sliding manner, one end of the mounting block (151) is connected with one end of the sliding rod (154) through a tension spring (155), the liquid supply pipe (150) and the air supply pipe (153) are respectively arranged on the corresponding mounting blocks (151), and magnets which are mutually adsorbed are arranged at one ends of the liquid supply pipe (150), the air supply pipe (153) and the connecting pipe (145);
further comprises: a first rack (17) located on the left side of the first conveyor belt (12);
the circumference of the first conveyer belt (12) is fixedly connected with a connecting block (13) corresponding to the clamping hoop (134), the connecting block (13) is rotationally connected with a rotating shaft (131), the clamping hoop (134) is fixedly connected with the rotating shaft (131), the rotating shaft (131) is fixedly connected with a gear (132), and the gear (132) is meshed with a first rack (17) so as to turn over the capillary tube (14) to enable the first port (140) to enter the liquid inlet area (3) when upwards;
further comprises: the second rack (18) is positioned on the right side of the liquid inlet area (3), and a liquid control area (4) is arranged between the liquid inlet area (3) and the second rack (18) and is used for preventing the extraction liquid in the first port (140) of the capillary tube (14) from flowing out;
the second rack (18) is used to turn the capillary (14) over again, causing the first port (140) to be downward.
2. The cell detection device based on the paper-based discharge technology according to claim 1, wherein the sealing cover (142) is provided with a slot (143) and a liquid storage groove (144), the sleeve (141) corresponds to the slot (143), and the second port (1401) at one end of the capillary tube (14) corresponds to the liquid storage groove (144).
3. The paper-based discharge technology-based cell detection device of claim 1, wherein the clamping hoops (134) are symmetrically disposed on both sides of the first conveyor belt (12).
4. The cell detection device based on the paper-based discharge technology according to claim 1, wherein an upper mounting frame (2) is fixedly connected to the base support (1), a second conveying belt (21) is rotatably arranged on the upper mounting frame (2), a positioning jig (22) is fixedly connected to the outer wall of the second conveying belt (21), an opening (23) is formed in one side of the positioning jig (22), and the positioning jig (22) is used for positioning and placing paper.
5. A method of using a paper-based discharge technology based cell detection device comprising the paper-based discharge technology based cell detection device of claim 1, comprising the steps of:
s1, installing a capillary tube (14) on a clamping hoop (134), driving a plurality of clamping hoops (134) on a first conveying belt (12) to rotate through rotation of the first conveying belt (12), and driving the capillary tube (14) to move towards the lower side of the first conveying belt (12) after the rotation of the first conveying belt (12), so that a first port (140) is downward and enters an extraction liquid storage box (6), and an extraction liquid in the extraction liquid storage box (6) enters the capillary tube (14);
s2, when the capillary tube (14) with the extract liquid moves to the liquid inlet area (3), the liquid inlet area (3) discharges dehydrated cells into the capillary tube (14);
s3, when the capillary tube (14) with the extraction liquid and the dehydrated cells moves to the air source supply area (5), air is supplied to the capillary tube (14) through the connecting pipe (145), the dehydrated cells are extracted upwards by the extraction liquid by the air entering the capillary tube (14), the extracted cell extraction product is sprayed onto paper from the first port (140) of the capillary tube (14), the conductive clamp connected with the paper discharges, the cell extraction product is ionized, ions are sprayed out from the tip of the paper and enter the sample inlet (81) of the mass spectrometer (8) for detection, and then the cell detection is completed.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111397985A (en) * | 2020-04-14 | 2020-07-10 | 清华大学 | Single cell mass spectrometry |
| CN111965093A (en) * | 2020-10-26 | 2020-11-20 | 宁波华仪宁创智能科技有限公司 | Single cell mass spectrometry device and method |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN112444583A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Cell detection device and method based on paper-based discharge technology |
| CN112444479A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Single cell mass spectrometry system and method based on parallel processing technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111397985A (en) * | 2020-04-14 | 2020-07-10 | 清华大学 | Single cell mass spectrometry |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN111965093A (en) * | 2020-10-26 | 2020-11-20 | 宁波华仪宁创智能科技有限公司 | Single cell mass spectrometry device and method |
| CN112444583A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Cell detection device and method based on paper-based discharge technology |
| CN112444479A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Single cell mass spectrometry system and method based on parallel processing technology |
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