CN206441693U - Mass spectrometry system - Google Patents
Mass spectrometry system Download PDFInfo
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- CN206441693U CN206441693U CN201621486814.1U CN201621486814U CN206441693U CN 206441693 U CN206441693 U CN 206441693U CN 201621486814 U CN201621486814 U CN 201621486814U CN 206441693 U CN206441693 U CN 206441693U
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- mass spectrometry
- bearing part
- spectrometry system
- guide rail
- ion gun
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- 238000004949 mass spectrometry Methods 0.000 title claims abstract description 24
- 238000012546 transfer Methods 0.000 claims abstract description 16
- 238000005070 sampling Methods 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 10
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 6
- 238000012360 testing method Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012188 paraffin wax Substances 0.000 claims description 4
- 230000035515 penetration Effects 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 description 12
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000001514 detection method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 102000015427 Angiotensins Human genes 0.000 description 3
- 108010064733 Angiotensins Proteins 0.000 description 3
- YAJCHEVQCOHZDC-QMMNLEPNSA-N actrapid Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3N=CNC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@H](C)CC)[C@H](C)CC)[C@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C(N)=O)C1=CNC=N1 YAJCHEVQCOHZDC-QMMNLEPNSA-N 0.000 description 3
- 239000002416 angiotensin derivative Substances 0.000 description 3
- LEMUFSYUPGXXCM-JNEQYSBXSA-N caninsulin Chemical compound [Zn].C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC3N=CN=C3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1C=NC=N1 LEMUFSYUPGXXCM-JNEQYSBXSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000011261 inert gas Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000001601 dielectric barrier discharge ionisation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000005220 pharmaceutical analysis Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
Abstract
The utility model provides a kind of mass spectrometry system, and mass spectrometry system includes ion gun, sampling device and mass spectrograph；The sampling device includes：Guide rail；Support, the support is suitable to move on the guide rail；Bearing part, the two ends of the bearing part are fixed on the bracket, and the bearing part uses hydrophobic material；At least two transfer units, the transfer unit uses water wetted material, including Part I, the connection Part I and the Part II extending into container being arranged on the bearing part；Adjacent transfer unit is not contacted；Container, the container is set on the bracket, inner containment testing sample；Drive module, the drive module drives the support to be moved on guide rail so that the central axis of the exit portal of the ion gun passes through the Part I.Utility model has the advantages that accuracy is good, sensitivity is high.
Description
Technical field
The utility model is related to mass spectral analysis, more particularly to mass spectrometry system.
Background technology
Open type mass ion source is the new mass ion source that can be used under atmospheric pressure open environment, without complexity
Sample pre-treatments, can realize the in situ, real-time of sample, fast ionic in very short time, with miniature portable, inspection
Survey cost it is low, it is applied widely the advantages of, in crowds such as food inspection, Pharmaceutical Analysis, environmental monitoring, public safety and clinical diagnosises
It is multi-field to have broad application prospects.
At present, when carrying out fluid sample analysis using open type mass ion source (DBDI and LTP etc.), typically with capillary
Or glass bar realizes sample feeding.Due to there are a large amount of high energy particles in the plasma that ejects, using it is such enter sample prescription
Background noise is higher during formula, and is difficult to the analysis of large biological molecule sample, such as polypeptide, protein.Using paper substrate sample introduction
Mode, can eliminate part high energy particle in plasma, weaken ionization energy, reduce ambient noise, improve analysis sensitive
Degree.This mode has many deficiencies, such as：
1. hand sampling can only be used, there is larger interference from human factor, and sample automation, high flux can not be realized
Analysis；
3. keeping the moistening in paper substrate hydrophilic area (sample dropwise addition area) to be more conducive to the ionization of sample in experimentation, obtain more
High sensitivity for analysis.But the fluid sample in paper substrate hydrophilic area is added dropwise and only has 3-10 μ L, highly volatile, it is impossible in array analysis
It is middle to keep the moistening of each hydrophilic area, namely sensitivity for analysis and repeatability can not be ensured, it is impossible to realize high throughput analysis.
Utility model content
To solve the deficiency in above-mentioned prior art, the utility model provides a kind of automatic sampling, sensitivity
High, reproducible mass spectrometry system.
The purpose of this utility model is achieved through the following technical solutions：
A kind of mass spectrometry system, the mass spectrometry system includes ion gun, sampling device and mass spectrograph；The sample introduction
Device includes：
Guide rail；
Support, the support is suitable to move on the guide rail；
Bearing part, the two ends of the bearing part are fixed on the bracket, and the bearing part uses hydrophobic material；
At least two transfer units, the transfer unit uses water wetted material, including be arranged on the bearing part
A part, the connection Part I and the Part II extending into container；Adjacent transfer unit is not contacted；
Container, the container is set on the bracket, inner containment testing sample；
Drive module, the drive module drives the support to be moved on guide rail so that the exit portal of the ion gun
Central axis pass through the Part I.
Compared with prior art, the utility model have the advantage that for：
1. by drive module it is automatic, accurately control the movement of Part I on support so that on each Part I
Sample is ionized respectively, eliminates the interference that human factor is caused, and is improved precision of analysis, is realized automation and the height of sample
Flux distribution；
2. Part I can be ensured for a long time during the analysis of sample on (Part I of array arrangement)
Moistening, is conducive to the ionization of testing sample, ensures sensitivity and the repeatability of analysis；
3. device is simple, easy to operate, detection efficiency can be lifted.
Brief description of the drawings
Referring to the drawings, disclosure of the present utility model will be easier to understand.Skilled addressee readily understands that
It is：These accompanying drawings are used only for illustrating the technical solution of the utility model, and are not intended to protection of the present utility model
Scope is construed as limiting.In figure：
Fig. 1 is the structure diagram of the mass spectrometry system according to the utility model embodiment 1；
Fig. 2 is the structure diagram of the sampling device according to the utility model embodiment 1；
Fig. 3 is the corresponding mass spectrogram of actrapid monotard according to the utility model embodiment 4；
Fig. 4 is the corresponding mass spectrogram of angiotensins according to the utility model embodiment 5.
Embodiment
Fig. 1-4 and following description describe optional embodiment of the present utility model how to instruct those skilled in the art
Implement and reproduce the utility model.In order to instruct technical solutions of the utility model, simplify or eliminate some conventional aspects.This
Art personnel should be appreciated that modification or replacement from these embodiments will be in the range of the utility model.This area
Technical staff should be appreciated that following characteristics can combine to form multiple modifications of the present utility model in a variety of ways.Thus, originally
Utility model is not limited to following optional embodiments, and is only limited by claim and their equivalent.
Embodiment 1：
Fig. 1 schematically illustrates the structure diagram of the mass spectrometry system of the utility model embodiment, as shown in Fig. 1,
The mass spectrometry system includes：
Ion gun, mass spectrograph, the ion gun, mass spectrograph are separately positioned on the both sides of sampling device；
Fig. 2 schematically illustrates the structure diagram of the sampling device of the utility model embodiment, as shown in Fig. 2 described
Sampling device includes：
Guide rail 11；The guide rail is horizontally disposed with
Support 21, the support is suitable to move horizontally on the guide rail；
Bearing part 31, the bearing part is vertically arranged, and left and right ends are fixed on the bracket, and the bearing part is used
Hydrophobic material；
At least two transfer units, the transfer unit uses water wetted material, including be arranged on the bearing part
A part 32, the connection Part I and the Part II 33 extending into container；Adjacent transfer unit is not contacted；It is each
Individual transfer unit is vertically arranged, and multiple transfer units are arranged on the bearing part from left to right；It is provided with the load bearing member
The part of Part I has through hole；
Container 41, the container is set on the bracket, inner containment testing sample；
Drive module (not shown), such as stepper motor, the drive module drive the support to be moved on guide rail so that
The central axis of the exit portal of the ion gun passes through the Part I.
The method of work according to above-mentioned mass spectrometry system of the utility model embodiment, the method for work include with
Lower step：
(A1) capillarity is utilized, the sample accommodated in the container of different testing samples is respectively delivered to each Part I,
The various samples of Part I correspondence；
(A2) driving arm is moved on guide rail so that the plasma that the ion gun is projected is mapped to described first
Point, sample is ionized；
(A3) spectrometer analysis ion, into step (A2), until having surveyed all samples one by one.
Embodiment 2：
The mass spectrometry system of the utility model embodiment, as different from Example 1：
1. through hole is no longer set on the load bearing member.
2. described in guide rail be vertically arranged, the upper and lower ends of bearing part are fixed on support, and Part I is set from top to down
Put on the load bearing member.By moving up and down bearing part so that different Part I are respectively between ion gun and mass spectrograph.
Embodiment 3：
The mass spectrometry system of the utility model embodiment, as different from Example 1：
Rectangle filter paper is cut out, and there is a plurality of banded structure filter being connected with rectangle filter paper in the sidepiece of rectangle filter paper
Paper；Paraffin is printed on rectangle filter paper, but reserves on rectangle filter paper multiple border circular areas, border circular areas and banded structure filter paper
Connecting portion reserve, it is not print paraffin to reserve region at these；The border circular areas prints paraffin as Part I
Region is used as the bearing part for carrying Part I.
Embodiment 4：
Application examples of the mass spectrometry system in actrapid monotard detects described in the utility model embodiment 3.
In the application examples, a diameter of 4mm of Part I, Part II a width of 1.5mm, a length of 8mm；Shuttle is
1mL polyethylene centrifuge tubes；Ion gun uses open type ion gun, and nozzle is 1.5cm with mass spectrograph injection port distance；Sampling device
It is 0.2mm, ion gun nozzle, first apart from mass spectrograph injection port distance between ion gun nozzle and mass spectrograph injection port
The partial center of circle and mass spectrograph injection port are located in same horizontal line.Mass Spectrometer Method m/z scopes are set to 1,000 2000, ion gun
Heating-up temperature is set to 100 degree, and inert gas flow velocity is set to 3L/min, and detection sample is 10ppm actrapid monotard's standard sample (solvents
For methanol：Water=1:1 mixed solution, containing 0.1% acetic acid), corresponding mass spectrogram is as shown in Figure 3.Therefrom it can be observed
Two echo signal peaks of 1453.18 (four electric charges of band) and 1937.00 (three electric charges of band), intensity be respectively 3.81e2 and
5.01e2, signal to noise ratio is 16.0 and 34.2.Four measured value relative standard deviations are 7.23% obtained by mobile motor.
Embodiment 5：
Application examples of the mass spectrometry system in angiotensins detection described in the utility model embodiment 3.
In the application examples, a diameter of 3mm of Part I, Part II a width of 1.5mm, a length of 10mm；Shuttle
For 1mL polyethylene centrifuge tubes；Ion gun nozzle is 1.5cm with mass spectrograph injection port distance；Sampling device is located at ion gun nozzle
It is 0.2mm, ion gun nozzle, the center of circle of Part I and matter apart from mass spectrograph injection port distance between mass spectrograph injection port
Spectrometer injection port is located in same horizontal line.Mass Spectrometer Method m/z scopes are set to 400 1100, and ion source heating temperature is set to 100
DEG C, inert gas flow velocity is set to 3L/min, and detection sample is that (solvent is methanol to 10ppm angiotensins standard sample：Water=1:
1 mixed solution, containing 0.1% acetic acid), corresponding mass spectrogram is as shown in Figure 4.524.09 (two electric charges of band) therefrom can be observed
With 1046.82 (one electric charge of band) two echo signal peaks, intensity is respectively 2.92e2 and 2.81e2, and signal to noise ratio is 5.8 Hes
12.6.Four measured value relative standard deviations are 9.65% obtained by mobile motor.
Claims (7)
1. a kind of mass spectrometry system, the mass spectrometry system includes ion gun, sampling device and mass spectrograph；Its feature exists
In：The sampling device includes：
Guide rail；
Support, the support is suitable to move on the guide rail；
Bearing part, the two ends of the bearing part are fixed on the bracket, and the bearing part uses hydrophobic material；
At least two transfer units, the transfer unit uses water wetted material, including first be arranged on the bearing part
Divide, connect the Part I and the Part II extending into container；Adjacent transfer unit is not contacted；
Container, the container is set on the bracket, inner containment testing sample；
Drive module, the drive module drives the support to be moved on guide rail so that in the exit portal of the ion gun
Heart axis passes through the Part I.
2. mass spectrometry system according to claim 1, it is characterised in that：The bearing part and Part I are filter paper
Different piece, the partial penetration hydrophobic material of non-Part I on filter paper.
3. mass spectrometry system according to claim 1, it is characterised in that：The hydrophobic material is paraffin.
4. mass spectrometry system according to claim 1, it is characterised in that：The guide rail is horizontally disposed with, the bearing part,
Transfer unit is vertically arranged, horizontal direction arrangement of the transfer unit along bearing part.
5. mass spectrometry system according to claim 1, it is characterised in that：The guide rail, bearing part are vertically arranged, described
Part I is arranged on the bearing part from top to down.
6. mass spectrometry system according to claim 1, it is characterised in that：The Part I is rounded, a diameter of 3-
5mm circle, Part II is in a width of 1-1.5mm, a length of 5-10mm strip.
7. mass spectrometry system according to claim 1, it is characterised in that：The ion gun is open type ion gun.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621486814.1U CN206441693U (en) | 2016-12-31 | 2016-12-31 | Mass spectrometry system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621486814.1U CN206441693U (en) | 2016-12-31 | 2016-12-31 | Mass spectrometry system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206441693U true CN206441693U (en) | 2017-08-25 |
Family
ID=59642191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621486814.1U Active CN206441693U (en) | 2016-12-31 | 2016-12-31 | Mass spectrometry system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206441693U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106960777A (en) * | 2016-12-31 | 2017-07-18 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system and its method of work |
| CN111670485A (en) * | 2018-02-02 | 2020-09-15 | 托夫沃克股份公司 | Automatic sampler |
-
2016
- 2016-12-31 CN CN201621486814.1U patent/CN206441693U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106960777A (en) * | 2016-12-31 | 2017-07-18 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system and its method of work |
| WO2018121205A1 (en) * | 2016-12-31 | 2018-07-05 | 宁波大学 | Mass spectrometry analysis system and working method and application thereof, and sampling device |
| CN106960777B (en) * | 2016-12-31 | 2019-08-20 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system and its working method |
| US11011365B2 (en) | 2016-12-31 | 2021-05-18 | Ningbo University | Mass spectrometry system and working method and application thereof, and sampling device used therein |
| CN111670485A (en) * | 2018-02-02 | 2020-09-15 | 托夫沃克股份公司 | Automatic sampler |
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Address after: West side of 1st floor, 1st floor, Building A, No. 288 Jingu Middle Road (East), Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region after: China Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee before: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region before: China |
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