CN110484446A - A kind of fluid circulation exchange method suitable for biological products large-scale production - Google Patents
A kind of fluid circulation exchange method suitable for biological products large-scale production Download PDFInfo
- Publication number
- CN110484446A CN110484446A CN201910811620.6A CN201910811620A CN110484446A CN 110484446 A CN110484446 A CN 110484446A CN 201910811620 A CN201910811620 A CN 201910811620A CN 110484446 A CN110484446 A CN 110484446A
- Authority
- CN
- China
- Prior art keywords
- container
- liquid
- intergrade
- circulation
- circulation pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B23/00—Pumping installations or systems
- F04B23/04—Combinations of two or more pumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
- F04B49/06—Control using electricity
Abstract
The present invention relates to biological product technical fields, in particular a kind of fluid circulation suitable for biological products large-scale production exchanges method, including container, the bottom centre of the container is equipped with gas inlet passages, base level feed pathway is fixed on the right side wall of the container and on gas inlet passages, the other end of the base level feed pathway is connected to the input terminal of base level liquid circulation pump, the output end of the base level liquid circulation pump is equipped with base level circulation apocenosis passage, the present invention realizes fluid motion by design, liquid continues in realization biologics production large capacity reactor, efficient circulation, realize nutriment in production process, the lasting supplement of gas, fluid motion, realize nutriment in biologics production process, dissolved gas and cell effectively exchange and pass through fluid motion, Avoid mechanical shock or damage of the tradition machinery blender to bio-carrier.
Description
Technical field
The present invention relates to biological product technical field, specially a kind of fluid suitable for biological products large-scale production is followed
Ring exchanges method.
Background technique
Biologics, such as oncolytic virus (using cell as culture produce carrier), UCART, be now in high speed research and development with
Qualify Phase, it is main that manual mode is relied on to carry out sample preparation in development phase.The drug of present clinical test, soon
The use of scale of input in the future, the biologics of every batch of production should be able to meet making for tens of patients even patients up to a hundred
With, 50 to 200 liters of the liquid measure of every batch of pharmaceutical production, the volume of Ji Geng great.In the production process of large capacity, the life of most critical
Production. art requires to be to ensure to seek in technical process with culture as the cell of direct drug product or pharmaceutical production host cell
Dissolved gas etc. in feeding substance, culture solution is effectively contacted and is exchanged.Conventional antibodies pharmaceutical production fermentor or biology are anti-
Answer device although and may be implemented the liquid agitation and mixing in large-rolume container, but its rigid leaflet chip mechanical agitator institute for using
The mechanical shear stress of generation is also easy to produce the damage and death of cell.The novel biologics large-scale production based on cell, needs
One Large Copacity production reactor efficient, without mechanical damage damage force, safety stirs and evenly mixs system, realizes new bio
The large-scale production of drug.
In conclusion the present invention proposes that a kind of fluid circulation suitable for biological products large-scale production exchanges method to solve
The problem of certainly existing.
Summary of the invention
The purpose of the present invention is to provide a kind of fluid circulations suitable for biological products large-scale production to exchange method, with
Solve the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme:
A kind of fluid circulation exchange method suitable for biological products large-scale production, including container, the bottom of the container
Portion center is equipped with gas inlet passages, is fixed with basis on the right side wall of the container and on gas inlet passages
Grade feed pathway, the other end of the base level feed pathway are connected to the input terminal of base level liquid circulation pump, the basis
The output end of grade liquid circulation pump is equipped with base level circulation apocenosis passage, and the other end of the base level circulation apocenosis passage connects
It connects on the right side wall of container, the base level recycles the lower section of apocenosis passage and is located at and is connected on the right side wall of container
Intergrade feed pathway, the other end of the intergrade feed pathway are connected between a grade liquid circulation pump, the intergrade liquid
The output end of body circulation pump is equipped with intergrade circulation apocenosis passage, and the other end of the intergrade circulation apocenosis passage is connected to
On the right side wall of container, the intergrade circulation apocenosis passage is equipped with intergrade switch valve, and the intergrade recycles drain
The lower section in channel and it is located on the right side wall of container and is fixed with intergrade loop branches apocenosis passage, the intergrade circulation
The other end connection of branch's apocenosis passage is solid on the intergrade loop branches apocenosis passage on intergrade circulation apocenosis passage
Surely intergrade branch switch valve, the lower section of the intergrade loop branches apocenosis passage and the right side wall for being located at container are equipped with
It is equipped with top input duct, the other end of the top input duct is mounted on the input terminal of top liquid circulation pump, described
The output end of top liquid circulation pump is connected with top-level loop apocenosis passage, the other end connection of the top-level loop apocenosis passage
On the right side wall of container, the bottom inside the container is fixed with culture medium and enters channel, the side wall inside the container
It is equipped with circulation flow-disturbing partition.
Preferably, the model of the base level liquid circulation pump, intergrade liquid circulation pump and top liquid circulation pump is equal
For BS300-1C peristaltic pump and be electrically connected on PLC system control cabinet by conducting wire.
Preferably, the intergrade switch valve and intergrade branch switch valve are N4v410-420-15 and lead to
Conducting wire is crossed to be electrically connected on PLC system control cabinet.
Preferably, the input terminal of the base level liquid circulation pump, intergrade liquid circulation pump and top liquid circulation pump
Connected with output end pipeline and pipeline connection structure be based on container be axisymmetrical be arranged two groups, the culture medium into
Enter channel and is based on two groups of gas inlet passages setting.
Preferably, the circulation flow-disturbing partition be based on gas inlet passages be arranged two groups and two groups of gas inlet passages it
Between in the Inner Constitution liquid downlink cycle channel of container, gas inlet passages described in two groups are constituted between container inside respectively
Liquid uplink laminar flow channel.
Preferably, the base level feed pathway, base level circulation apocenosis passage, intergrade feed pathway, intergrade are followed
Ring branch apocenosis passage and top input duct are connectivity structure with container and channel is followed successively by staggered floor from top to bottom and arranges.
Preferably, the intergrade circulation apocenosis passage sequentially passes through container, circulation flow-disturbing partition from right to left and prolongs
Extend to the left side of circulation flow-disturbing partition.
Preferably, the top-level loop apocenosis passage is through to the inside of container and extends to a left side for circulation flow-disturbing partition
Side, top-level loop apocenosis passage is located at the top of circulation flow-disturbing partition in the container inside.
Preferably, comprising the following steps:
S1, circulation flow-disturbing partition forms circular ring shape liquid and rises laminar region in shell of tank and container, operates PLC at this time
System control cabinet is operated by control base level liquid circulation pump, intergrade liquid circulation pump and top liquid circulation pump, thus
Using the channel being separately connected on base level liquid circulation pump, intergrade liquid circulation pump and top liquid circulation pump, by container
The whole laminar flow of bottom liquid to container top is promoted, and base level liquid circulation pump, intergrade liquid circulation pump and top
The adjacent two-stage apocenosis passage in the channel being separately connected on liquid circulation pump and feed pathway carry out staggered floor arrangement, bottom level-one
Apocenosis passage realizes that two-stage avoids nutrients in effective exchange of exchange area up and down on the feed pathway top of upper layer level-one
The distribution of the localized non-uniformities of matter and dissolved gas, forms steady and sustained liquid uplink laminar flow channel；
S2, bottom upper aq is exchanged to container top center, while bottom by top-level cycle liquid discharge passage in container
Layer circulating pump is from container bottom centre pumping liquid, and the lasting liquid of bottom is pumped out to be pumped into the lasting liquid of top layer, in container
Central area forms steady and sustained liquid downlink cycle channel, the circulation flow-disturbing partition in container prevent data feedback channel liquid with
The mixing of down going channel liquid；
S3, S1, in upper aq channel and S2 in descending liquid channel form stable liquid circulation loop, realize
In container the circulation within the scope of whole container inner cavity such as nutriment, dissolved gas with exchange.
Compared with prior art, the beneficial effects of the present invention are:
1, in the present invention, the liquid circulation of device without mixing is realized by design, avoid tradition machinery blender to cell and
The impact and damage of other biological carrier.
2, in the present invention, the non-uniform problem of Flow Field Distribution is brought by designing optimization conventional leaf blender significantly,
It greatly improves nutriment, the uniformity that dissolved gas is distributed inside container, realizes the more efficient effect in culture production process
Benefit and the shaft and sealing for exempting conventional leaf blender, full cavity and pipeline realize stringent sealing, and pollution is effectively reduced
Risk.
3, in the present invention, height independence can be kept by the design pump housing and liquid line, be convenient for vessel and liquid
Circulation line designs and produces into disposable consumption class product, further increases the safety of production process, intersects between reduction batch
The risk of pollution and setting by different pump stages, can different liquids volume in flexible adaptation production process, ensure different
High efficiency under volume
4, in the present invention, fluid motion is realized by design, realizes liquid in biologics production large capacity reactor
Continue, efficient circulation, realize that the lasting supplement of nutriment, gas in production process, fluid motion realize that biologics are raw
Nutriment, dissolved gas effectively exchange and pass through fluid motion with cell during production, avoid tradition machinery blender
Mechanical shock or damage to bio-carrier.
Detailed description of the invention
Fig. 1 is the whole schematic view of the front view of the present invention；
Fig. 2 is circulation canal principle schematic view of the front view of the present invention；
Fig. 3 is that circulation canal schematic diagram of the present invention overlooks figure structure schematic representation.
In figure: 1- container, 2- gas inlet passages, 3- base level feed pathway, 4- base level liquid circulation pump, the basis 5-
Grade circulation apocenosis passage, 6- intergrade feed pathway, 7- intergrade liquid circulation pump, 8- intergrade circulation apocenosis passage, in 9-
Intercaste switch valve, 10- intergrade loop branches apocenosis passage, 11- intergrade branch switch valve, the top input duct of 12-,
The top liquid circulation pump of 13-, 14- top-level loop apocenosis passage, 15- culture medium enter channel, 16- circulation flow-disturbing partition.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution:
A kind of fluid circulation exchange method suitable for biological products large-scale production, including container 1, the bottom of container 1
Center is equipped with gas inlet passages 2, is fixed with base level on the right side wall of container 1 and on gas inlet passages 2
Feed pathway 3, the other end of base level feed pathway 3 are connected to the input terminal of base level liquid circulation pump 4, and base level liquid follows
The output end of ring pump 4 is equipped with base level circulation apocenosis passage 5, and the other end of base level circulation apocenosis passage 5 is connected to container 1
Right side wall on, base level circulation apocenosis passage 5 lower section and be located at intergrade feed liquor is connected on the right side wall of container 1
Channel 6, the other end of intergrade feed pathway 6 are connected between grade liquid circulation pump 7, the output of intergrade liquid circulation pump 7
End is equipped with intergrade circulation apocenosis passage 8, and the other end of intergrade circulation apocenosis passage 8 is connected on the right side wall of container 1,
Intergrade recycles apocenosis passage 8 and is equipped with intergrade switch valve 9, and intergrade, which recycles the lower section of apocenosis passage 8 and is located at, to be held
Be fixed with intergrade loop branches apocenosis passage 10 on the right side wall of device 1, intergrade loop branches apocenosis passage 10 it is another
End connection is fixed with intergrade branch on intergrade loop branches apocenosis passage 10 and cuts on intergrade circulation apocenosis passage 8
Change valve 11, the lower section of the intergrade loop branches apocenosis passage 10 and right side wall for being located at container 1 is equipped with top inlet tube
Road 12, the other end of top input duct 12 are mounted on the input terminal of top liquid circulation pump 13, top liquid circulation pump 13
Output end is connected with top-level loop apocenosis passage 14, and the other end of top-level loop apocenosis passage 14 is connected to the right side wall of container 1
On, the bottom inside container 1 is fixed with culture medium and enters channel 15, and the side wall inside container 1 is equipped with circulation flow-disturbing partition
16。
Workflow of the present invention: in base level liquid circulation pump 4, intergrade liquid circulation pump 7, top liquid circulation pump
13, intergrade switch valve 9 and intergrade branch switch valve 11 and PLC system control is electrically connected at by conducting wire respectively
Under the action of on cabinet, energization processing is carried out to equipment, at this time on base level liquid circulation pump 4, intergrade liquid circulation pump 7 and top
It is axis pair that the structure of pipeline and pipeline connection that the input terminal of grade liquid circulation pump 13 is connected with output end, which is based on container 1,
Claim setting two groups, culture medium enter channel 15 be based on gas inlet passages 2 be arranged two groups, circulation flow-disturbing partition 16 be based on gas into
Enter channel 2 to be arranged between two groups and two groups of gas inlet passages 2 in the Inner Constitution liquid downlink cycle channel of container 1, two
Group gas inlet passages 2 constitute liquid uplink laminar flow channel, base level feed pathway 3, base level between 1 inside of container respectively
Recycle apocenosis passage 5, intergrade feed pathway 6, intergrade loop branches apocenosis passage 10 and top input duct 12 with appearance
Device 1 is connectivity structure and channel is followed successively by staggered floor arrangement from top to bottom, and intergrade circulation apocenosis passage 8 successively passes through from right to left
Container 1, circulation flow-disturbing partition 16 and the left side for extending to circulation flow-disturbing partition 16 are worn, top-level loop apocenosis passage 14 is through to
The inside of container 1 and the left side for extending to circulation flow-disturbing partition 16, top-level loop apocenosis passage 14 is located at and follows in 1 inside of container
Under the action of the top of ring flow-disturbing partition 16, at the same 1 bottom and top of container install culture medium channel 15 (or other culture
Production use liquid), gas inlet passages 2, realize nutriment, dissolved gas lasting supply.
Fluid circulation exchanges method such as Fig. 2, such as Fig. 3, and specific step is as follows:
S1, circulation flow-disturbing partition 16 forms circular ring shape liquid and rises laminar region in 1 shell of container and container 1, grasps at this time
Make PLC system control cabinet and passes through control base level liquid circulation pump 4, intergrade liquid circulation pump 7 and top liquid circulation pump 13
Running, thus using being separately connected on base level liquid circulation pump 4, intergrade liquid circulation pump 7 and top liquid circulation pump 13
Channel, the whole laminar flow at 1 bottom liquid of container to the top of container 1 is promoted, and base level liquid circulation pump 4, intergrade
The adjacent two-stage apocenosis passage in the channel being separately connected on liquid circulation pump 7 and top liquid circulation pump 13 and feed pathway carry out
Staggered floor arrangement, the apocenosis passage of bottom level-one realize that two-stage is in exchange area up and down on the feed pathway top of upper layer level-one
Effectively exchange avoids nutriment and the localized non-uniformities of dissolved gas from being distributed, forms steady and sustained liquid uplink laminar flow
Channel；
S2, bottom upper aq is exchanged to 1 top center of container by top-level cycle liquid discharge passage in container 1, simultaneously
From 1 bottom centre's pumping liquid of container, the lasting liquid of bottom is pumped out to be pumped into bottom circulating pump with the lasting liquid of top layer, is being held
1 central area of device forms steady and sustained liquid downlink cycle channel, and the circulation flow-disturbing partition 16 in container 1 prevents data feedback channel
The mixing of liquid and down going channel liquid；
S3, S1, in upper aq channel and S2 in descending liquid channel form stable liquid circulation loop, realize
In container 1 circulation within the scope of 1 inner cavity of whole container such as nutriment, dissolved gas with exchange.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of fluid circulation suitable for biological products large-scale production exchanges method, including container (1), it is characterised in that:
The bottom centre of the container (1) is equipped with gas inlet passages (2), on the right side wall of the container (1) and close to gas
It is fixed with base level feed pathway (3) on into channel (2), the other end of the base level feed pathway (3) is connected to base
The input terminal of plinth grade liquid circulation pump (4), the output end of the base level liquid circulation pump (4) are equipped with base level circulation drain
The other end in channel (5), base level circulation apocenosis passage (5) is connected on the right side wall of container (1), and the base level is followed
The lower section of ring apocenosis passage (5) and be located at be connected on the right side wall of container (1) intergrade feed pathway (6), it is described in
The other end of intercaste feed pathway (6) is connected between a grade liquid circulation pump (7), the intergrade liquid circulation pump (7) it is defeated
Outlet is equipped with intergrade circulation apocenosis passage (8), and the other end of intergrade circulation apocenosis passage (8) is connected to container
(1) on right side wall, intergrade circulation apocenosis passage (8) is equipped with intergrade switch valve (9), the intergrade circulation
The lower section of apocenosis passage (8) and be located at container (1) right side wall on be fixed with intergrade loop branches apocenosis passage (10),
The other end connection of the intergrade loop branches apocenosis passage (10) is in intergrade circulation apocenosis passage (8), the centre
Intergrade branch switch valve (11), the intergrade loop branches drain are fixed on grade loop branches apocenosis passage (10)
The lower section in channel (10) and be located at container (1) right side wall be equipped with top input duct (12), the top input duct
(12) the other end is mounted on the input terminal of top liquid circulation pump (13), and the output end of the top liquid circulation pump (13) connects
It is connected to top-level loop apocenosis passage (14), the other end of the top-level loop apocenosis passage (14) is connected to the right side of container (1)
On wall, the internal bottom of the container (1) is fixed with culture medium and enters channel (15), on the internal side wall of the container (1)
Equipped with circulation flow-disturbing partition (16).
2. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the base level liquid circulation pump (4), intergrade liquid circulation pump (7) and top liquid circulation pump (13) model
It is the peristaltic pump of BS300-1C and is electrically connected on PLC system control cabinet by conducting wire respectively.
3. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the intergrade switch valve (9) and intergrade branch switch valve (11) are N4v410-420-15 and pass through
Conducting wire is electrically connected on PLC system control cabinet.
4. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the input of the base level liquid circulation pump (4), intergrade liquid circulation pump (7) and top liquid circulation pump (13)
Holding the pipeline connected with output end and the structure of pipeline connection to be based on container (1) is that axisymmetrical is arranged two groups, the training
Feeding base enters channel (15) and is based on two groups of gas inlet passages (2) setting.
5. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the circulation flow-disturbing partition (16) is based on gas inlet passages (2) and two groups and two groups of gas inlet passages (2) is arranged
Between in the Inner Constitution liquid downlink cycle channel of container (1), gas inlet passages described in two groups (2) respectively with container (1)
Liquid uplink laminar flow channel is constituted between inside.
6. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the base level feed pathway (3), base level circulation apocenosis passage (5), intergrade feed pathway (6), intergrade are followed
Ring branch apocenosis passage (10) and top input duct (12) be with container (1) connectivity structure and channel from top to bottom successively
For staggered floor arrangement.
7. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: intergrade circulation apocenosis passage (8) sequentially pass through from right to left container (1), circulation flow-disturbing partition (16) and
Extend to the left side of circulation flow-disturbing partition (16).
8. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, special
Sign is: the top-level loop apocenosis passage (14) is through to the inside of container (1) and extends to circulation flow-disturbing partition (16)
Left side, top-level loop apocenosis passage (14) is located at the top of circulation flow-disturbing partition (16) in container (1) inside.
9. a kind of fluid circulation suitable for biological products large-scale production according to claim 1 exchanges method, including
Following steps:
S1, circulation flow-disturbing partition (16) forms circular ring shape liquid and rises laminar region in container (1) shell and container (1), at this time
Operation PLC system control cabinet is followed by control base level liquid circulation pump (4), intergrade liquid circulation pump (7) and top liquid
Ring pumps (13) running, to utilize base level liquid circulation pump (4), intergrade liquid circulation pump (7) and top liquid circulation pump
(13) channel being separately connected on promotes the whole laminar flow at the top of container (1) bottom liquid to container (1), and base level
The channel being separately connected on liquid circulation pump (4), intergrade liquid circulation pump (7) and top liquid circulation pump (13) it is adjacent two
Grade apocenosis passage and feed pathway carry out staggered floor arrangement, the apocenosis passage of bottom level-one on the feed pathway top of upper layer level-one,
Realize that two-stage avoids nutriment and the localized non-uniformities of dissolved gas from being distributed, shape in effective exchange of exchange area up and down
At steady and sustained liquid uplink laminar flow channel；
Bottom upper aq is exchanged to container (1) top center by S2, the interior top-level cycle liquid discharge passage of container (1), simultaneously
Bottom circulating pump is from container (1) bottom centre pumping liquid, and the lasting liquid of bottom is pumped out to be pumped into the lasting liquid of top layer, In
Container (1) central area forms steady and sustained liquid downlink cycle channel, and the circulation flow-disturbing partition (16) in container (1) prevents
The mixing of data feedback channel liquid and down going channel liquid；
The descending liquid channel in upper aq channel and S2 in S3, S1 forms stable liquid circulation loop, realizes container
(1) circulation within the scope of whole container (1) inner cavity such as interior nutriment, dissolved gas with exchange.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910811620.6A CN110484446A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulation exchange method suitable for biological products large-scale production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910811620.6A CN110484446A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulation exchange method suitable for biological products large-scale production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110484446A true CN110484446A (en) | 2019-11-22 |
Family
ID=68555313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910811620.6A Pending CN110484446A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulation exchange method suitable for biological products large-scale production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110484446A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB741652A (en) * | 1950-08-23 | 1955-12-07 | Ruhrchemie Ag | Method and apparatus for the treatment of gases with liquids |
| CN2561501Y (en) * | 2002-08-06 | 2003-07-23 | 深圳市清泉水系统工程设备有限公司 | Dissolving mixers |
| CN203663728U (en) * | 2013-11-15 | 2014-06-25 | 宝丰县楚城白雀陈醋厂 | Liquid materialcirculating stirring device |
| CN208485824U (en) * | 2018-06-06 | 2019-02-12 | 江苏富淼科技股份有限公司 | A kind of circulating production system of dimethyl diallyl ammonium chloride |
| CN109415935A (en) * | 2016-06-23 | 2019-03-01 | S.P.M.流量控制股份有限公司 | Hydraulic frac system, device and method |
-
2019
- 2019-08-30 CN CN201910811620.6A patent/CN110484446A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB741652A (en) * | 1950-08-23 | 1955-12-07 | Ruhrchemie Ag | Method and apparatus for the treatment of gases with liquids |
| CN2561501Y (en) * | 2002-08-06 | 2003-07-23 | 深圳市清泉水系统工程设备有限公司 | Dissolving mixers |
| CN203663728U (en) * | 2013-11-15 | 2014-06-25 | 宝丰县楚城白雀陈醋厂 | Liquid materialcirculating stirring device |
| CN109415935A (en) * | 2016-06-23 | 2019-03-01 | S.P.M.流量控制股份有限公司 | Hydraulic frac system, device and method |
| CN208485824U (en) * | 2018-06-06 | 2019-02-12 | 江苏富淼科技股份有限公司 | A kind of circulating production system of dimethyl diallyl ammonium chloride |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103589638B (en) | Pneumatic self-circulation animal cell culture bioreactor and application method thereof | |
| CN101732770B (en) | Dual-cavity liquid storage tank for biological artificial liver support system | |
| CN105219635A (en) | A kind of built-in light source air lift type inner ring stream photosynthesis physiological target | |
| CN103877631A (en) | Bioartificial liver system | |
| CN101768545B (en) | Cycling elution biomimetic peristaltic enzymolysis reactor and method thereof | |
| CN108315220A (en) | A kind of bio-pharmaceuticals Zymolysis Equipment based on electromagnetism feed flow principle | |
| CN103170021A (en) | Deflector-type micro-capsule suspension type fluidized bed bioreactor for artificial liver | |
| CN110484446A (en) | A kind of fluid circulation exchange method suitable for biological products large-scale production | |
| CN204999909U (en) | Circulation photobioreactor in built in light source gas lift formula | |
| CN108060081A (en) | A kind of multi-layer planar filling type bioreactor | |
| CN215887065U (en) | Circulation tangential flow perfusion cell culture system | |
| CN115786112A (en) | Differential pressure regulating system, continuous harvesting system and using method thereof | |
| CN108384702A (en) | A kind of bio-pharmaceuticals Zymolysis Equipment with opposite mixed function | |
| CN106834117B (en) | For releasing the enzyme reactor and intelligent enzyme reaction work station of zooblast attaching | |
| CN211284347U (en) | Efficient multi-liquid-medicine liquid injection device and chromosome harvesting and processing system thereof | |
| CN208701056U (en) | Degerming fermentor | |
| CN207786000U (en) | Basket-hanging cycle extraction unit | |
| CN201551639U (en) | Dual-cavity liquid storage tank of bioartificial liver support system | |
| CN110484445A (en) | A kind of fluid circulating device suitable for the production of scale biological products | |
| CN211689068U (en) | Organic acid circulating fermentation device using sterile air as power | |
| CN206127326U (en) | A fixed bed device for cell culture | |
| CN218666101U (en) | Differential pressure regulating system and continuous harvesting system | |
| CN212883868U (en) | A belt cleaning device for mesenchymal stem cell preparation | |
| CN201343538Y (en) | Liquid removing device for culturing microcarrier cells | |
| CN110684642A (en) | Three-dimensional perfusion type cell culture instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191122 |