CN211689068U

CN211689068U - Organic acid circulating fermentation device using sterile air as power

Info

Publication number: CN211689068U
Application number: CN201922269907.9U
Authority: CN
Inventors: 徐庆阳; 李国华; 李燕军; 张成林; 马倩; 谢希贤; 范晓光; 陈宁
Original assignee: Tianjin University of Science and Technology
Current assignee: Tianjin University of Science and Technology
Priority date: 2019-12-17
Filing date: 2019-12-17
Publication date: 2020-10-16
Anticipated expiration: 2029-12-17

Abstract

The utility model provides an organic acid circulation fermentation device taking sterile air as power, which comprises a fermentation tank No. 1, an ejector, a cyclone separator and a fermentation tank No. 2, wherein the bottom outlet of the fermentation tank No. 1 is communicated with the feed liquid inlet of the ejector, the feed liquid outlet of the ejector is communicated with the feed liquid inlet of the cyclone separator, the light phase outlet of the cyclone separator is communicated with the fermentation tank No. 2, the heavy phase outlet of the cyclone separator is used for discharging solid materials, and all devices are connected through pipelines; the device simple structure, convenient operation can realize organic acid circulation fermentation and preliminary separation and extraction, is favorable to practicing thrift manufacturing cost, improves production efficiency.

Description

Organic acid circulating fermentation device using sterile air as power

Technical Field

The utility model relates to an organic acid circulation fermentation technical field, especially an use organic acid circulation fermenting installation of aseptic air as power.

Background

The cyclic fermentation is a novel fermentation technology, fermentation equipment and fermentation process, and research is underway. Utility model has been reported in the patent database: a tyrosine fermentation and extraction device (application number: CN201821494178.6) uses a centrifugal pump to provide a flowing driving force of fermentation liquor, a disc centrifuge separates crystals and bacterial liquid, the crystals are extracted, and the bacterial liquid enters another fermentation tank for fermentation after being concentrated, thereby realizing circular fermentation. However, the above device has the following problems: because some crystals (taking tyrosine as an example) are very fine and broken, the discs in the narrow space of the disc-type centrifuge are very easy to block, so that the continuous operation is difficult; the centrifugal pump and the disc type centrifugal machine have complicated internal structures and have dead corners, high-temperature steam is difficult to thoroughly sterilize, and fermentation liquor is easy to contaminate bacteria.

The ejector utilizes a compressed air working medium. The liquid is sucked by the low pressure generated at the outlet of the nozzle by the high-speed air flow, and then the high-speed air drives the liquid to advance at high speed. The cyclone separator is a centrifugal settling separation device for feed liquid rotating under the action of pressure, utilizes the difference of density of solid phase and liquid phase in centrifugal force in centrifugal field, the centrifugal force applied to solid phase is large, overcomes water resistance, moves along the wall of the separator, moves downwards under the action of gravity field, and is discharged by a discharge valve at the bottom of the separator; the liquid phase is subjected to small centrifugal force, moves upwards along the gravity center of the cyclone separator under the compression movement of the outer solid phase, and is discharged through a liquid phase outlet; and the inner parts of the ejector and the cyclone separator are a large cavity, so that the structure is simple, and thorough sterilization is easy.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the utility model is to provide an organic acid circulating fermentation device using sterile air as power.

In order to solve the technical problem, the technical scheme of the utility model is that:

the utility model provides an use organic acid circulation fermenting installation of aseptic air as power, includes fermentation cylinder No. 1, sprayer, cyclone and fermentation cylinder No. 2, the bottom export of fermentation cylinder No. 1 communicates the feed liquid import of sprayer, the feed liquid export of sprayer communicates cyclone's feed liquid import, cyclone's light phase export communicates fermentation cylinder No. 2, cyclone's heavy phase export is used for discharging solid-state material, passes through the pipe connection between each equipment.

Preferably, the pipeline of the organic acid circulation fermentation device taking sterile air as power is provided with a pressure gauge, a flow meter and a valve for detecting and adjusting pressure and flow rate.

According to the organic acid circulating fermentation device taking sterile air as power, the whole set of device can be used for high-pressure steam sterilization operation, and equipment is kept at positive pressure during working, so that a sterile environment is kept.

Preferably, in the above organic acid circulation fermentation device using sterile air as power, a discharge valve or an air-lock valve is arranged at the heavy phase outlet of the cyclone separator for automatic discharge, so as to meet the requirement that the separation factor Fr is 100-. The cyclone separator separates the bacteria liquid and the crystal in the fermentation liquid, and part of the crystal in the separated bacteria liquid is not separated and is directly connected to the fermentation tank No. 2.

According to the ejector of the organic acid circulating fermentation device taking the sterile air as power, the sterile air inlet is communicated with high-pressure sterile air, the sterile air forms low pressure in the vacuum chamber of the ejector pump, the fermentation liquor at the feed liquid inlet is sucked, and the high-speed sterile air atomizes the fermentation liquor and enables the fermentation liquor to advance at high speed.

The power source of the fermentation liquor in the organic acid circulating fermentation device taking sterile air as power mainly has two aspects: the fermentation tank No. 1 is a mechanical stirring ventilation fermentation tank, a large amount of sterile air is introduced in the fermentation process, the tank pressure is generally 0.1-0.4MPa, and the liquid level height of the fermentation liquid also forms static pressure; secondly, in the sprayer, the high-speed sterile air can atomize the fermentation liquor and move at high speed. The fermentation liquor moving at high speed tangentially enters the cyclone separator at higher speed and performs centrifugal motion, and because the density of crystals is greater than the density of bacteria liquid and greater than the density of gas, the fermentation liquor in the cyclone separator can generate a layering phenomenon under the action of centrifugal force, and the crystals and the air of the bacteria liquid are sequentially arranged from the inner wall to the light phase outlet of the cyclone separator; the crystals can be deposited at the bottom of the cyclone separator under the dual actions of centrifugal force and gravity and are continuously discharged through a discharge valve; the bacteria liquid is driven by sterile air to be discharged out of the cyclone separator through a light phase outlet of the cyclone separator.

Has the advantages that:

the organic acid circulation fermentation device taking sterile air as power has the advantages of simple structure, convenience in operation, capability of realizing organic acid circulation fermentation and preliminary separation extraction, contribution to saving production cost and improvement of production efficiency.

Drawings

FIG. 1 is a schematic structural diagram of an organic acid circulating fermentation device powered by sterile air according to the present invention;

FIG. 2 is a schematic diagram of the injector configuration;

FIG. 3 is a schematic diagram of a cyclone separator configuration;

FIG. 4 is a schematic cross-sectional view of the cyclonic separator taken along the line of FIG. 3A-A.

In the figure: fermentation tank No. 1.1; 2. an ejector; 3. a cyclone separator; no. 4.2 fermenter; 2-1, an ejector sterile air inlet; 2-2, a feed liquid outlet of the ejector; 2-3, injector feed liquid inlet; 3-1, a feed liquid inlet of the cyclone separator; 3-2, a light phase outlet of the cyclone separator; 3-3, a discharge valve of the cyclone separator; and 3-4, a heavy phase outlet of the cyclone separator.

Detailed Description

Example 1

As shown in figures 1-4, the organic acid circulating fermentation device using sterile air as power comprises a fermentation tank 1 No. 1, an ejector 2, a cyclone separator 3 and a fermentation tank 4 No. 2, wherein the bottom outlet of the fermentation tank 1 is communicated with a feed liquid inlet 2-3 of the ejector, the sterile air inlet 2-1 of the ejector is communicated with high-pressure sterile air, the sterile air forms low pressure in a vacuum chamber of an injection pump, the fermentation liquid at the feed liquid inlet is sucked, the high-speed sterile air atomizes the fermentation liquid and enables the fermentation liquid to advance at high speed, the feed liquid outlet 2-2 of the ejector is communicated with the feed liquid inlet 3-1 of the cyclone separator, the light phase outlet 3-2 of the cyclone separator is communicated with the fermentation tank 2 No. 3, the heavy phase outlet 3-4 of the cyclone separator is used for discharging solid materials, a discharge valve 3-3 (or an air-closing device) is arranged at the heavy phase outlet of the, the device is used for automatic discharge and meets the requirement that the separation factor Fr is 100-. The cyclone separator separates the bacteria liquid and the crystal in the fermentation liquid, and part of the crystal in the separated bacteria liquid is not separated and is directly connected to the fermentation tank No. 2. The devices are connected through pipelines, pressure gauges, flow meters and valves can be arranged on the pipelines and used for detecting and adjusting pressure and flow rate, the whole device can perform high-pressure steam sterilization operation, and the devices are kept at positive pressure during working to keep an aseptic environment.

Example 2

Taking the tyrosine circulating fermentation process as an example, the organic acid circulating fermentation device taking sterile air as power is further described in detail, and the following tyrosine producing bacteria are escherichia coli:

when the production rate of tyrosine in the fermentation tank No. 1 is reduced, a part of tyrosine fermentation liquor flows into the ejector from the bottom under the action of pressure, high-speed sterile air drives the fermentation liquor in the ejector vacuum chamber to quickly (15-30m/s) tangentially enter the cyclone separator, crystals and bacteria liquid are separated in the cyclone separator, the crystals are gathered at the bottom of the cyclone separator and are discharged through a discharge valve, meanwhile, the tyrosine crystals are collected, the bacteria liquid enters the fermentation tank No. 2 from a light phase outlet of the cyclone separator for fermentation, and the sterile air in the bacteria liquid is discharged through a tail gas pipeline of the fermentation tank No. 2.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. An organic acid circulation fermentation device taking sterile air as power is characterized in that: including fermentation cylinder 1, sprayer, cyclone and fermentation cylinder 2, the bottom export intercommunication sprayer of fermentation cylinder 1 feed liquid import, the feed liquid export intercommunication cyclone's of sprayer feed liquid import, cyclone's light phase export intercommunication fermentation cylinder 2, cyclone's heavy phase export is used for discharging solid material, passes through the pipe connection between each equipment.

2. The organic acid circulation fermentation device using sterile air as power according to claim 1, wherein: the pipeline is provided with a pressure gauge, a flowmeter and a valve.

3. The organic acid circulation fermentation device using sterile air as power according to claim 1, wherein: and a discharge valve or an air-lock valve is arranged at a heavy phase outlet of the cyclone separator.