CN114797215A - Sorting and filtering device and method for tumor cell clusters - Google Patents
Sorting and filtering device and method for tumor cell clusters Download PDFInfo
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- CN114797215A CN114797215A CN202210531384.4A CN202210531384A CN114797215A CN 114797215 A CN114797215 A CN 114797215A CN 202210531384 A CN202210531384 A CN 202210531384A CN 114797215 A CN114797215 A CN 114797215A
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- 238000001914 filtration Methods 0.000 title claims abstract description 109
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000007664 blowing Methods 0.000 claims abstract description 55
- 239000012535 impurity Substances 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims description 22
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims 2
- 239000007788 liquid Substances 0.000 abstract description 16
- 206010028980 Neoplasm Diseases 0.000 abstract description 5
- 210000001519 tissue Anatomy 0.000 description 9
- 108010066057 cabin-1 Proteins 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 3
- 238000001959 radiotherapy Methods 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000009347 mechanical transmission Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/62—Regenerating the filter material in the filter
- B01D29/66—Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/76—Handling the filter cake in the filter for purposes other than for regenerating
- B01D29/86—Retarding cake deposition on the filter during the filtration period, e.g. using stirrers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
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- Sampling And Sample Adjustment (AREA)
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Abstract
The invention discloses a device and a method for sorting and filtering tumor cell clusters in the technical field of tumors, and the device comprises a filtering bin and a flat blowing nozzle, wherein a filtering plate is fixedly connected to the middle part in the filtering bin, the flat blowing nozzle is positioned in the filtering bin, the upper end port of the flat blowing nozzle is attached to the lower surface of the filtering plate, the flat blowing nozzle is fixedly communicated with an air supply pipe, one end of the air supply pipe, far away from the flat blowing nozzle, extends out of the filtering bin and is connected with a flexible hose, and the air supply pipe is in sliding connection with the side wall of the filtering bin; the invention can form negative pressure in the area below the filter plate in the filter bin and positive pressure in the area above the filter plate in the filter bin, thereby accelerating the filtering speed of tumor cell clusters in the mixed liquid above the filter plate, and simultaneously repeatedly blowing out the impurities blocked in the filter holes of the filter plate upwards, thereby further improving the filtering speed of the tumor cell clusters.
Description
Technical Field
The invention relates to the technical field of tumors, in particular to a device and a method for sorting and filtering tumor cell clusters.
Background
Oncology, as well as internal medicine, surgery, gynecology and pediatrics, is a secondary discipline of clinical medicine. The oncology department is divided into the oncology department, the oncology radiotherapy department, the oncology surgery department and the like; the medical oncology is mainly used for medical treatment of various benign and malignant tumors; the tumor radiotherapy department is mainly engaged in the radiotherapy of tumors; the tumor surgery provides comprehensive treatment mainly based on operation.
In the working process, doctors in oncology department often need to sort and filter tumor cell clusters through a sorting and filtering device, namely, fresh tissue dispersion liquid is added into the sorting and filtering device to sort and filter the tumor cell clusters. Since the size of the larger tissues such as adipose tissue, membrane tissue, connective tissue, etc. in the tissue dispersion liquid is several hundred microns, and the size of the tumor cell mass in the tissue dispersion liquid is generally several tens of microns, the tissue with larger size often blocks the filter plate in the sorting filter device, and the sorting of the tumor cell mass is affected.
Most of the existing tumor cell mass sorting and filtering devices adopt a vibration method to avoid the blockage of the filter plate, however, water flow generated by vibration can only disperse deposits accumulated on the surface of the filter plate, and can not easily wash away sundries stuck in the upper end opening of the filter hole, especially the sundries stuck in the filter hole.
Based on the above, the invention designs a device and a method for sorting and filtering tumor cell masses, so as to solve the above problems.
Disclosure of Invention
The present invention aims to provide a device and a method for sorting and filtering tumor cell masses, so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a sorting and filtering device for tumor cell clusters comprises a filtering bin and a flat air blowing nozzle, wherein a filtering plate is fixedly connected to the middle part in the filtering bin, the flat air blowing nozzle is positioned in the filtering bin, the upper end of the flat blowing nozzle is attached to the lower surface of the filter plate, the flat blowing nozzle is fixedly communicated with an air supply pipe, one end of the blast pipe, which is far away from the flat blowing nozzle, extends to the outside of the filtering bin and is connected with a flexible hose, the air supply pipe is connected with the side wall of the filter bin in a sliding way, the bottom end of the flexible hose is fixedly communicated with an exhaust pipe through a shell, one end of the exhaust pipe far away from the shell extends into the filtering bin, a fan is rotationally connected in the shell, the outer wall of the air supply pipe is fixedly connected with a connecting rod positioned outside the filtering bin, one end of the connecting rod, far away from the air supply pipe, is fixedly connected with a bearing seat, and the bearing seat is connected with the filtering bin in a sliding manner; the bearing seat is in threaded connection with a reciprocating screw rod, and the reciprocating screw rod is rotationally connected with the filtering bin;
the filter comprises a filter cabin, a fan and a reciprocating screw rod, wherein the filter cabin is arranged in the filter cabin, the reciprocating screw rod is arranged in the filter cabin, the fan is arranged in the filter cabin, the reciprocating screw rod is arranged in the filter cabin, the fan is arranged in the filter cabin, and the reciprocating screw rod is connected with the reciprocating screw rod.
As a further proposal of the invention, the follow-up anti-blocking mechanism comprises a shell and a wave-shaped chute, the lower surface of the sleeve is attached to the upper surface of the filter plate, the lower end of the sleeve is provided with an air inlet groove aligned with the upper port of the flat air blowing nozzle, the inner wall of the casing is connected with a gas-collecting hood in a sliding way in the up-down direction, the left and right side walls of the gas-collecting hood are symmetrically provided with exhaust grooves, the left side wall and the right side wall of the casing are symmetrically provided with injection channels, the upper ports of the injection channels can be communicated with the exhaust grooves at the same side, the outer wall of the casing is fixedly connected with a slide bar parallel to the blast pipe, one end of the slide bar, which is far away from the casing, extends to the outside of the filtering bin and is fixedly connected with a connecting rod, the wavy chute is arranged on the inner wall of the filtering bin, and the inner wall of the wavy chute is connected with a sliding block in a sliding manner, the sliding block is fixedly connected with a gas collecting hood, and the upper side of the gas collecting hood is connected with a passive stirring mechanism positioned on the upper side of the injection channel.
As a further scheme of the present invention, the driving mechanism includes a motor, the motor is fixedly connected to an outer wall of the filter bin, an output shaft of the motor is fixedly connected to the reciprocating screw rod, an output shaft of the motor is fixedly connected to a first bevel gear, the first bevel gear is engaged with a second bevel gear, the second bevel gear is rotatably connected to an outer wall of the filter bin, a rotating shaft of the second bevel gear is fixedly connected to a third bevel gear, the third bevel gear is engaged with a fourth bevel gear, the fourth bevel gear is rotatably connected to an outer wall of the filter bin, a rotating shaft of the fourth bevel gear is fixedly connected to a worm, the worm is engaged with a scroll rim, and the scroll rim is fixedly connected to a rotating shaft of the fan.
As a further scheme of the invention, the passive stirring mechanism comprises a first cross rod and a twisted rotating shaft, the first cross rod and the twisted rotating shaft are both parallel to the air supply pipe, the first cross rod is connected with the inner wall of the filtering bin in a sliding manner in the vertical direction, the first cross rod is connected with a sliding sleeve in a sliding manner, the sliding sleeve is fixedly connected with the gas collecting hood, the first cross rod is hinged with a push rod, the twisted rotating shaft is connected with the inner wall of the filtering bin in a rotating manner, a plurality of stirring rods are fixedly connected to the circumferential wall of the twisted rotating shaft, the outer wall of the twisted part of the twisted rotating shaft is connected with a rotating sleeve in a rotating manner, and the outer wall of the rotating sleeve is fixedly connected with the push rod.
As a further scheme of the invention, an upward pushing mechanism is arranged at the lower side of the flat blowing nozzle in the filtering bin, the upward pushing mechanism comprises a second cross rod, the second cross rod is parallel to the air supply pipe and is fixedly connected with the inner wall of the filtering bin, the outer wall of the second cross rod is connected with a moving block in a sliding manner, the moving block is fixedly connected with a sleeve, the inner wall of the sleeve is connected with an ejector rod in a sliding manner, a spring is arranged between the lower end of the ejector rod and the inner bottom end of the sleeve, and the upper end of the ejector rod extends out of the sleeve and is fixedly connected with the bottom of the flat blowing nozzle.
As a further scheme of the invention, the upper end of the filter bin is provided with a sealing cover, the lower end of the filter bin is fixedly communicated with a funnel nozzle, and the lower end of the funnel nozzle is inserted with a collection test tube.
A sorting and filtering device for tumor cell clusters is characterized in that: one end of the exhaust pipe extending into the filter bin is bent downwards, and a horn cover is fixedly sleeved on the outer wall of the end, bent downwards, of the exhaust pipe.
A method of sorting and filtering tumor cell mass, the method comprising the steps of:
the method comprises the following steps: firstly opening the sealing cover, pouring tissue fluid containing tumor cell clusters on the upper side of the filter plate into the filter bin, and then closing the sealing cover:
step two: then a driving mechanism is started, the driving mechanism drives a fan to exhaust air from the lower side area of the filter plate in the filter bin, the flat blowing nozzle uses the exhausted air to suck away impurities blocked in the filter hole of the filter plate and the upper port, and meanwhile, positive and negative pressure difference is formed in the areas on the upper side and the lower side of the filter plate, so that the filtering effect is improved;
step three: the driving mechanism drives the follow-up anti-blocking mechanism at the same time, so that the follow-up anti-blocking mechanism can collect and pressurize the gas blown by the flat blowing nozzle and jet the gas to the upper surface of the filter plate once at intervals so as to blow away deposits clinging to the upper port of the filter hole of the filter plate;
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism can stir the tissue fluid on the upper side of the filter plate, and further impurities are prevented from being accumulated;
step five: the tumor cell mass separated by filtration is collected by a collecting test tube at last, and the collecting test tube is taken down after the filtration is finished, so that the tumor cell mass can be conveniently researched and used in the later period.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can form negative pressure in the area below the filter plate in the filter bin and positive pressure in the area above the filter plate in the filter bin, thereby accelerating the filtering speed of tumor cell clusters in the mixed liquid above the filter plate, and simultaneously repeatedly blowing out the impurities blocked in the filter holes of the filter plate upwards, thereby further improving the filtering speed of the tumor cell clusters.
2. The follow-up anti-blocking mechanism can collect air blown by the flat blowing nozzle and form certain pressure, accumulated air can be rapidly sprayed to the upper surface of the filter plate at intervals, and deposits attached to the upper end openings of the filter holes of the filter plate can be blown away by the high-speed sprayed air, so that the filtering efficiency of the device is improved.
3. The follow-up anti-blocking mechanism can drive the passive stirring mechanism to stir the mixed liquid on the upper side of the filter plate in the filter bin, so that excessive impurities can be prevented from being accumulated on the upper surface of the filter plate, and the filtering efficiency of the filter bin is further improved.
Drawings
FIG. 1 is a front elevational view of the overall construction of the present invention;
FIG. 2 is a front bottom view of the overall structure of the present invention;
FIG. 3 is a schematic cross-sectional view of a filtration cartridge and its internal structure;
FIG. 4 is an enlarged view of a portion of FIG. 3A;
FIG. 5 is an enlarged view of a portion of FIG. 3 at B;
FIG. 6 is an enlarged view of a portion of FIG. 3 at C;
FIG. 7 is an enlarged view of a portion of FIG. 3 at D;
FIG. 8 is a cross-sectional view of the cannula and its internal structure;
FIG. 9 is a flow chart of the method of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a filtering bin; 2. a filter plate; 3. a flat air blowing nozzle; 4. an air supply pipe; 5. a flexible hose; 6. an exhaust pipe; 7. a connecting rod; 8. a bearing seat; 9. a reciprocating screw rod; 10. a housing; 11. an air inlet groove; 12. a gas-collecting hood; 13. an exhaust groove; 14. a jet path; 15. a slide bar; 16. a wavy chute; 17. a slider; 18. a first cross bar; 19. a sliding sleeve; 20. a push rod; 21. twisting the rotating shaft; 22. a stirring rod; 23. rotating the sleeve; 24. a housing; 25. a scroll ring; 26. a fan; 27. a motor; 28. a first bevel gear; 29. a second bevel gear; 30. a third bevel gear; 31. a fourth bevel gear; 32. a worm; 33. a second cross bar; 34. a moving block; 35. a sleeve; 36. a top rod; 37. a spring; 38. a sealing cover; 39. a funnel mouth; 40. collecting the test tubes; 41. a horn cover.
Detailed Description
Referring to fig. 1-9, the present invention provides a technical solution: a sorting and filtering device for tumor cell clusters comprises a filtering bin 1 and a flat blowing nozzle 3, wherein a filtering plate 2 is fixedly connected to the middle part in the filtering bin 1, the flat blowing nozzle 3 is positioned in the filtering bin 1, the upper end of the flat blowing nozzle 3 is attached to the lower surface of the filtering plate 2, the flat blowing nozzle 3 is fixedly communicated with an air supply pipe 4, one end, far away from the flat blowing nozzle 3, of the air supply pipe 4 extends out of the filtering bin 1 and is connected with a flexible hose 5, the air supply pipe 4 is in sliding connection with the side wall of the filtering bin 1, the bottom end of the flexible hose 5 is fixedly communicated with an air exhaust pipe 6 through a shell 24, one end, far away from the shell 24, of the air exhaust pipe 6 extends into the filtering bin 1, a fan 26 is connected in the shell 24 in a rotating manner, the outer wall of the air supply pipe 4 is fixedly connected with a connecting rod 7 positioned outside the filtering bin 1, one end, far away from the air supply pipe 4, of the connecting rod 7 is fixedly connected with a bearing seat 8, the bearing seat 8 is connected with the filtering bin 1 in a sliding way; the bearing seat 8 is in threaded connection with a reciprocating screw rod 9, and the reciprocating screw rod 9 is rotationally connected with the filtering bin 1;
the filter comprises a filter cabin 1 and is characterized in that a driving mechanism is arranged outside the filter cabin 1, the driving mechanism can drive a reciprocating screw rod 9 to rotate and can drive a fan 26 to rotate to exhaust air, and a follow-up anti-blocking mechanism located on the upper side of a filter plate 2 is arranged in the filter cabin 1.
When the scheme is put into practical use, firstly, a proper amount of mixed liquid is poured into the upper side of the filter plate 2 in the filter bin 1, the liquid with tumor cell clusters in the mixed liquid slowly passes through the filter plate 2 to be filtered downwards, then the driving mechanism is started, the driving mechanism drives the reciprocating screw rod 9 to rotate, the reciprocating screw rod 9 drives the bearing seat 8 to reciprocate left and right, the bearing seat 8 drives the connecting rod 7 to move, the connecting rod 7 drives the air supply pipe 4 to move, the air supply pipe 4 drives the flat blowing nozzle 3 to reciprocate left and right, meanwhile, the driving mechanism drives the fan 26 to rotate, the fan 26 pumps out air on the lower side of the filter plate 2 through the exhaust pipe 6, so that the area on the lower side of the filter plate 2 in the filter bin 1 forms negative pressure, and simultaneously, the pumped air sequentially passes through the telescopic hose 5, the air supply pipe 4 and the flat blowing nozzle 3 and is blown out to the filter plate 2 through the upper port of the flat blowing nozzle 3, the air can blow out the blockages inside the filtering holes of the filtering plate 2 and at the upper end after being pressurized by the flat upper end of the flat blowing nozzle 3, and can form positive pressure in the area above the filtering plate 2 in the filtering chamber 1, thus, the invention can form negative pressure in the area below the filtering plate 2 in the filtering chamber 1 and form positive pressure in the area above the filtering plate 2 in the filtering chamber 1, thereby accelerating the filtering speed of tumor cell clusters in the mixed liquid at the upper side of the filtering plate 2, and simultaneously can repeatedly blow out the sundries blocked inside the filtering holes of the filtering plate 2 upwards, thereby further improving the filtering speed of the tumor cell clusters.
As a further scheme of the invention, the follow-up anti-blocking mechanism comprises a sleeve shell 10 and a wave-shaped sliding chute 16, the lower surface of the sleeve shell 10 is attached to the upper surface of the filter plate 2, the lower end of the sleeve shell 10 is provided with an air inlet groove 11 aligned with the upper port of the flat blowing nozzle 3, the inner wall of the sleeve shell 10 is connected with an air collecting hood 12 in a vertical direction in a sliding way, the left side wall and the right side wall of the air collecting hood 12 are symmetrically provided with exhaust grooves 13, the left side wall and the right side wall of the sleeve shell 10 are internally and symmetrically provided with injection channels 14, the upper port of the injection channel 14 can be communicated with the exhaust grooves 13 at the same side, the outer wall of the sleeve shell 10 is fixedly connected with a sliding rod 15 parallel to the air supply pipe 4, one end of the sliding rod 15 far away from the sleeve shell 10 extends to the outside of the filter bin 1 and is fixedly connected with a connecting rod 7, the wave-shaped sliding chute 16 is arranged on the inner wall of the filter bin 1, and the inner wall of the wave-shaped sliding chute 16 is connected with a sliding block 17 in a sliding way, the sliding block 17 is fixedly connected with the gas-collecting hood 12, and the upper side of the gas-collecting hood 12 is connected with a passive stirring mechanism positioned on the upper side of the injection channel 14.
When the scheme is put into practical use, when the connecting rod 7 moves left and right in a reciprocating manner, the connecting rod 7 simultaneously drives the sliding rod 15 to move, the sliding rod 15 drives the sleeve shell 10 to move synchronously with the flat blowing nozzle 3, air blown by the flat blowing nozzle 3 can enter the gas collecting hood 12 through the air inlet slot 11 after passing through the filtering holes of the filter plate 2, the gas collecting hood 12 can move left and right in a reciprocating manner along the sleeve shell 10, the gas collecting hood 12 can slide up and down in a reciprocating manner along the wavy sliding slot 16 through the sliding block 17 while moving horizontally, when the gas collecting hood 12 rises to the highest end area, the exhaust slot 13 can be aligned with the upper end of the injection channel 14, before that, the air in the gas collecting hood 12 can be accumulated to form a certain pressure, and after the exhaust slot 13 can be aligned with the upper end of the injection channel 14, the air accumulated in the gas collecting hood 12 can be sprayed out to the upper surface of the quick filter plate 2 through the injection channel 14, like this, follow-up anti-blocking mechanism can collect the air that the platykurtic blowing nozzle 3 blown up and form certain pressure, can be at intervals with the air of overstocking to 2 upper surfaces blowout of filter fast, high-speed spun air can blow away and hug closely filter 2 and cross the deposit of port department on the filtration pore to the device filtration efficiency has been improved.
As a further scheme of the present invention, the driving mechanism includes a motor 27, the motor 27 is fixedly connected with the outer wall of the filter bin 1, the output shaft of the motor 27 is fixedly connected with the reciprocating screw rod 9, the output shaft of the motor 27 is fixedly connected with a first bevel gear 28, the first bevel gear 28 is engaged with a second bevel gear 29, the second bevel gear 29 is rotatably connected with the outer wall of the filter bin 1, the rotating shaft of the second bevel gear 29 is fixedly connected with a third bevel gear 30, the third bevel gear 30 is engaged with a fourth bevel gear 31, the fourth bevel gear 31 is rotatably connected with the outer wall of the filter bin 1, the rotating shaft of the fourth bevel gear 31 is fixedly connected with a worm 32, the worm 32 is engaged with a worm wheel 25, and the worm wheel 25 is fixedly connected with the rotating shaft of the fan 26; in operation, the motor 27 is started, the motor 27 drives the reciprocating screw rod 9 to rotate through a rotating shaft of the motor 27, the reciprocating screw rod 9 causes the flat air blowing nozzle 3 and the casing 10 to synchronously reciprocate through a series of mechanical transmissions, meanwhile, the motor 27 drives the first bevel gear 28 to rotate, the first bevel gear 28 drives the second bevel gear 29 to rotate, the second bevel gear 29 drives the third bevel gear 30 to rotate through a rotating shaft of the second bevel gear 29, the third bevel gear 30 drives the fourth bevel gear 31 to rotate, the fourth bevel gear 31 drives the worm 32 to rotate through a rotating shaft of the fourth bevel gear 31, and the worm 32 drives the vortex ring 25 to rotate, so that the air draft operation of the fan 26 can be realized.
As a further scheme of the invention, the passive stirring mechanism comprises a first cross bar 18 and a twisted rotating shaft 21, the first cross bar 18 and the twisted rotating shaft 21 are both parallel to the air supply pipe 4, the first cross bar 18 is connected with the inner wall of the filtering bin 1 in a sliding manner in the vertical direction, the first cross bar 18 is connected with a sliding sleeve 19 in a sliding manner, the sliding sleeve 19 is fixedly connected with the air collecting hood 12, the first cross bar 18 is hinged with a push rod 20, the twisted rotating shaft 21 is connected with the inner wall of the filtering bin 1 in a rotating manner, a plurality of stirring rods 22 are fixedly connected to the circumferential wall of the twisted rotating shaft 21, the outer wall of the twisted part of the twisted rotating shaft 21 is connected with a rotating sleeve 23 in a rotating manner, and the outer wall of the rotating sleeve 23 is fixedly connected with the push rod 20.
When the scheme is put into practical use, when the gas collecting hood 12 moves up and down in a reciprocating manner, the gas collecting hood 12 can drive the first cross rod 18 to move up and down in a reciprocating manner through the sliding sleeve 19, the first cross rod 18 drives the twisting rotating shaft 21 to rotate through the matching of the push rod 20 and the rotating sleeve 23, and the twisting rotating shaft 21 drives the stirring rods 22 to rotate, so that the follow-up anti-blocking mechanism can drive the driven stirring mechanism to stir mixed liquid on the upper side of the filter plate 2 in the filter bin 1, excessive impurities can be prevented from being accumulated on the upper surface of the filter plate 2, and the filtering efficiency of the invention is further improved.
As a further scheme of the invention, an upward pushing mechanism is arranged at the lower side of the flat blowing nozzle 3 in the filtering bin 1, the upward pushing mechanism comprises a second cross rod 33, the second cross rod 33 is parallel to the air supply pipe 4 and is fixedly connected with the inner wall of the filtering bin 1, the outer wall of the second cross rod 33 is connected with a moving block 34 in a sliding manner, the moving block 34 is fixedly connected with a sleeve 35, the inner wall of the sleeve 35 is connected with an ejector rod 36 in a sliding manner, a spring 37 is arranged between the lower end of the ejector rod 36 and the inner bottom end of the sleeve 35, and the upper end of the ejector rod 36 extends out of the sleeve 35 and is fixedly connected with the bottom of the flat blowing nozzle 3; during operation, the spring 37 and the push rod 36 can reciprocate left and right along with the flat air blowing nozzle 3 by the arrangement of the second cross bar 33 and the moving block 34; through the setting of spring 37, can make ejector pin 36 upwards push up flat blow nozzle 3 all the time, can make the last port of flat blow nozzle 3 hug closely the filter 2 lower surface all the time, can avoid the air to leak from the gap between flat blow nozzle 3 and the filter 2.
The upper end of the filter bin 1 is provided with a sealing cover 38, the lower end of the filter bin 1 is fixedly communicated with a funnel nozzle 39, and the lower end of the funnel nozzle 39 is inserted with a collection test tube 40; in the work, through the setting of sealed lid 38, can be convenient for pour mixed liquid into filter storehouse 1 in, through the setting of collecting test tube 40, can be convenient for collect the tumor cell group after filtering.
As a further scheme of the invention, one end of the exhaust pipe 6, which extends into the filter bin 1, is bent downwards, and the outer wall of the downward bent end of the exhaust pipe 6 is fixedly sleeved with a horn cover 41; in operation, the installation of the bell jar 41 can prevent liquid from being sucked into the exhaust pipe 6.
A method of sorting and filtering tumor cell mass, the method comprising the steps of:
the method comprises the following steps: firstly opening the sealing cover 38, pouring tissue fluid containing tumor cell clusters into the filtering bin 1 at the upper side of the filtering plate 2, and then closing the sealing cover 38;
step two: then, a driving mechanism is started, the driving mechanism drives a fan 26 to exhaust air from the lower side area of the filter plate 2 in the filter bin 1, the flat blowing nozzle 3 uses the exhausted air to suck impurities blocked in the filter holes and the upper end port of the filter plate 2, and meanwhile, positive and negative pressure difference is formed in the areas on the upper side and the lower side of the filter plate 2, so that the filtering effect is improved;
step three: the driving mechanism drives the follow-up anti-blocking mechanism at the same time, so that the follow-up anti-blocking mechanism can collect and pressurize the gas blown by the flat blowing nozzle 3 and jet the gas to the upper surface of the filter plate 2 once at intervals so as to blow away deposits clinging to the upper end openings of the filter holes of the filter plate 2;
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism can stir the tissue fluid on the upper side of the filter plate 2, and further impurities are prevented from being accumulated;
step five: the tumor cell mass separated by filtration is finally collected by the collecting test tube 40, and after the filtration is finished, the collecting test tube 40 is taken down so as to be convenient for later-stage research and use.
The working principle is as follows: pouring a proper amount of mixed liquid to the upper side of a filter plate 2 in a filter cabin 1, slowly filtering liquid with tumor cell clusters through the filter plate 2, starting a driving mechanism, driving a reciprocating screw rod 9 to rotate by the driving mechanism, driving a bearing seat 8 to reciprocate left and right by the reciprocating screw rod 9, driving a connecting rod 7 to move by the bearing seat 8, driving an air supply pipe 4 to move by the connecting rod 7, driving a flat blowing nozzle 3 by the air supply pipe 4 to reciprocate left and right, driving a fan 26 to rotate by the driving mechanism, pumping air at the lower side of the filter plate 2 out by the fan 26 through an exhaust pipe 6, forming negative pressure in the area at the lower side of the filter plate 2 in the filter cabin 1, simultaneously leading the pumped air to a telescopic hose 5, the air supply pipe 4 and the flat blowing nozzle 3 in sequence, blowing out to the filter plate 2 through an upper port of the flat blowing nozzle 3, and blowing out the blockage inside the filter hole and the upper port of the filter plate 2 after the air is pressurized through the flat upper port of the flat blowing nozzle 3 And the area on the upper side of the filter plate 2 in the filter bin 1 can form positive pressure, so that the invention can form negative pressure in the area on the lower side of the filter plate 2 in the filter bin 1 and form positive pressure in the area on the upper side of the filter plate 2 in the filter bin 1, thereby accelerating the filtering speed of tumor cell clusters in mixed liquid on the upper side of the filter plate 2, and simultaneously repeatedly blowing out impurities blocked in the filter holes of the filter plate 2 upwards, thereby further improving the filtering speed of the tumor cell clusters.
Claims (8)
1. A sorting and filtering device for tumor cell clusters is characterized in that: the filter comprises a filter bin (1) and a flat blowing nozzle (3), wherein a filter plate (2) is fixedly connected to the middle in the filter bin (1), the flat blowing nozzle (3) is located in the filter bin (1), the upper end of the flat blowing nozzle (3) is attached to the lower surface of the filter plate (2), the flat blowing nozzle (3) is fixedly communicated with an air supply pipe (4), one end, far away from the flat blowing nozzle (3), of the air supply pipe (4) extends out of the filter bin (1) and is connected with a flexible hose (5), the air supply pipe (4) is in sliding connection with the side wall of the filter bin (1), the bottom end of the flexible hose (5) is fixedly communicated with an air extraction pipe (6) through a shell (24), one end, far away from the shell (24), of the air extraction pipe (6) extends into the filter bin (1), and a fan (26) is rotationally connected to the shell (24), the outer wall of the air supply pipe (4) is fixedly connected with a connecting rod (7) positioned outside the filtering bin (1), one end, far away from the air supply pipe (4), of the connecting rod (7) is fixedly connected with a bearing seat (8), and the bearing seat (8) is connected with the filtering bin (1) in a sliding mode; the bearing seat (8) is in threaded connection with a reciprocating screw rod (9), and the reciprocating screw rod (9) is rotationally connected with the filtering bin (1);
the filter is characterized in that a driving mechanism is arranged outside the filter bin (1), the driving mechanism can drive the reciprocating screw rod (9) to rotate and can drive the fan (26) to rotate for air draft, and a follow-up anti-blocking mechanism located on the upper side of the filter plate (2) is arranged in the filter bin (1).
2. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: the follow-up anti-blocking mechanism comprises a casing (10) and a wave-shaped sliding groove (16), the lower surface of the casing (10) is attached to the upper surface of the filter plate (2), an air inlet groove (11) aligned with the upper end opening of the flat air blowing nozzle (3) is formed in the lower end of the casing (10), the inner wall of the casing (10) is connected with an air collecting hood (12) in a vertical sliding mode, air exhaust grooves (13) are symmetrically formed in the left side wall and the right side wall of the air collecting hood (12), injection channels (14) are symmetrically formed in the left side wall and the right side wall of the casing (10), the upper end opening of each injection channel (14) can be communicated with the air exhaust grooves (13) on the same side, a sliding rod (15) parallel to the air supply pipe (4) is fixedly connected to the outer wall of the casing (10), one end, far away from the casing (10), of the sliding rod (15) extends to the outside the filter bin (1) and is fixedly connected with a connecting rod (7), the wave-shaped sliding groove (16) is formed in the inner wall of the filter bin (1), and the inner wall of the wavy sliding groove (16) is connected with a sliding block (17) in a sliding manner, the sliding block (17) is fixedly connected with the gas collecting hood (12), and the upper side of the gas collecting hood (12) is connected with a passive stirring mechanism positioned on the upper side of the injection channel (14).
3. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: the driving mechanism comprises a motor (27), the motor (27) is fixedly connected with the outer wall of the filtering bin (1), an output shaft of the motor (27) is fixedly connected with the reciprocating screw rod (9), an output shaft of the motor (27) is fixedly connected with a first bevel gear (28), the first bevel gear (28) is engaged with a second bevel gear (29), the second bevel gear (29) is rotationally connected with the outer wall of the filter bin (1), a rotating shaft of the second bevel gear (29) is fixedly connected with a third bevel gear (30), the third bevel gear (30) is engaged with a fourth bevel gear (31), the fourth bevel gear (31) is rotationally connected with the outer wall of the filtering bin (1), a rotating shaft of the fourth bevel gear (31) is fixedly connected with a worm (32), the worm (32) is meshed with a scroll rim (25), and the scroll rim (25) is fixedly connected with a rotating shaft of the fan (26).
4. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: passive rabbling mechanism includes first horizontal pole (18) and distortion pivot (21), first horizontal pole (18) and distortion pivot (21) all are parallel with blast pipe (4), first horizontal pole (18) are in the upper and lower direction with filter storehouse (1) inner wall sliding connection, first horizontal pole (18) sliding connection has sliding sleeve (19), sliding sleeve (19) and gas collecting hood (12) fixed connection, first horizontal pole (18) articulate has push rod (20), distortion pivot (21) are connected with filter storehouse (1) inner wall rotation, fixedly connected with a plurality of puddler (22) on distortion pivot (21) circumference wall, the outer wall rotation at distortion pivot (21) distortion position is connected with changes cover (23), change cover (23) outer wall and push rod (20) fixed connection.
5. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: the filter bin is characterized in that an upper jacking mechanism is arranged on the lower side of the flat blowing nozzle (3) in the filter bin (1), the upper jacking mechanism comprises a second cross rod (33), the second cross rod (33) is parallel to the air supply pipe (4) and is fixedly connected with the inner wall of the filter bin (1), the outer wall of the second cross rod (33) is slidably connected with a moving block (34), the moving block (34) is fixedly connected with a sleeve (35), the inner wall of the sleeve (35) is slidably connected with an ejector rod (36), a spring (37) is arranged between the lower end of the ejector rod (36) and the inner bottom end of the sleeve (35), and the upper end of the ejector rod (36) extends out of the sleeve (35) and is fixedly connected with the bottom of the flat blowing nozzle (3).
6. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: the device is characterized in that a sealing cover (38) is arranged at the upper end of the filtering bin (1), a funnel nozzle (39) is fixedly communicated with the lower end of the filtering bin (1), and a collecting test tube (40) is inserted into the lower end of the funnel nozzle (39).
7. The apparatus for sorting and filtering tumor cell mass according to claim 1, wherein: one end of the exhaust pipe (6) extending into the filter bin (1) is bent downwards, and the outer wall of the bent end of the exhaust pipe (6) is fixedly sleeved with a horn cover (41).
8. A method for sorting and filtering tumor cell mass, which is suitable for the device for sorting and filtering tumor cell mass as claimed in claims 1-7, and comprises the following steps:
the method comprises the following steps: firstly opening the sealing cover (38), pouring tissue fluid containing tumor cell clusters into the filtering bin (1) from the upper side of the filtering plate (2), and then closing the sealing cover (38):
step two: then a driving mechanism is started, the driving mechanism drives a fan (26) to exhaust air from a lower side area of a filter plate (2) in the filter bin (1), the flat blowing nozzle (3) uses the exhausted air to suck impurities blocked in a filter hole and an upper port of the filter plate (2), and meanwhile, positive and negative pressure difference is formed in areas on the upper side and the lower side of the filter plate (2), so that the filtering effect is improved;
step three: the driving mechanism drives the follow-up anti-blocking mechanism at the same time, so that the follow-up anti-blocking mechanism can collect and pressurize the gas blown by the flat blowing nozzle (3) and jet the gas to the upper surface of the filter plate (2) once at intervals so as to blow away deposits attached to the upper ports of the filter holes of the filter plate (2);
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism can stir the tissue fluid on the upper side of the filter plate (2), and further impurities are prevented from being accumulated;
step five: the tumor cell mass separated by filtration is finally collected by a collecting test tube (40), and after the filtration is finished, the collecting test tube (40) is taken down so as to be convenient for later research and use.
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