CN114797215B - Sorting and filtering device and method for tumor cell mass - Google Patents
Sorting and filtering device and method for tumor cell mass Download PDFInfo
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- CN114797215B CN114797215B CN202210531384.4A CN202210531384A CN114797215B CN 114797215 B CN114797215 B CN 114797215B CN 202210531384 A CN202210531384 A CN 202210531384A CN 114797215 B CN114797215 B CN 114797215B
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- 238000001914 filtration Methods 0.000 title claims abstract description 65
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000007664 blowing Methods 0.000 claims abstract description 45
- 238000003756 stirring Methods 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 11
- 239000012535 impurity Substances 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 12
- 206010028980 Neoplasm Diseases 0.000 abstract description 4
- 210000001519 tissue Anatomy 0.000 description 9
- 230000000903 blocking effect Effects 0.000 description 4
- 238000001356 surgical procedure Methods 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000001959 radiotherapy Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 206010010904 Convulsion Diseases 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000009347 mechanical transmission Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/62—Regenerating the filter material in the filter
- B01D29/66—Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/76—Handling the filter cake in the filter for purposes other than for regenerating
- B01D29/86—Retarding cake deposition on the filter during the filtration period, e.g. using stirrers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
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- Sampling And Sample Adjustment (AREA)
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Abstract
The invention discloses a sorting and filtering device and a method for tumor cell clusters, which belong to the technical field of tumors, and comprise a filtering bin and a flat blowing nozzle, wherein a filter plate is fixedly connected to the middle part in the filtering bin; the invention can make the area of the lower side of the filter plate in the filter bin form negative pressure and make the area of the upper side of the filter plate in the filter bin form positive pressure, thereby accelerating the filtering speed of tumor cell mass in the mixed liquid of the upper side of the filter plate, and simultaneously repeatedly blowing out the sundries blocked in the filter holes of the filter plate upwards, thereby further improving the filtering speed of the tumor cell mass.
Description
Technical Field
The invention relates to the technical field of tumors, in particular to a sorting and filtering device and method for tumor cell clusters.
Background
Oncology is the secondary discipline of clinical medicine as well as internal medicine, surgery, gynaecology and obstetrics, and paediatrics. Oncology is divided into oncology, oncology radiotherapy, oncology surgery and the like; the oncology is mainly used for internal medicine treatment of various benign and malignant tumors; the oncology radiotherapy department mainly engages in the radiotherapy of tumors; tumor surgery provides a comprehensive treatment based on surgery.
In the working process, a doctor of oncology often needs to sort and filter the tumor cell mass through a sorting and filtering device, namely, fresh tissue dispersion liquid is added into the sorting and filtering device to sort and filter the tumor cell mass. Since the size of large tissues such as adipose tissue, membranous tissue, connective tissue, etc. in the tissue dispersion is hundreds of micrometers, and the size of tumor cell mass in the tissue dispersion is typically tens of micrometers, the large-sized tissues often block the filter plate in the sorting filter device, and the sorting of the tumor cell mass is affected.
Most of the existing tumor cell mass sorting and filtering devices adopt a vibration method to avoid the blocking of the filter plates, however, water flow generated by vibration only can break up the accumulated matters accumulated on the surfaces of the filter plates, sundries clamped into the upper ports of the filter holes cannot be easily washed away, and particularly sundries clamped into the filter holes cannot be washed away.
Based on the above, the invention designs a sorting and filtering device and a method for tumor cell mass to solve the above problems.
Disclosure of Invention
The invention aims to provide a sorting and filtering device and method for tumor cell clusters, which are used for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a sorting filter equipment of tumour cell group, includes filtration storehouse and platykurtic blowing nozzle, middle part fixedly connected with filter in the filtration storehouse, platykurtic blowing nozzle is located filtration storehouse, and platykurtic blowing nozzle upper port pastes with the filter lower surface mutually, platykurtic blowing nozzle fixedly communicates has the blast pipe, the one end that platykurtic blowing nozzle was kept away from to the blast pipe extends to outside the filtration storehouse and is connected with flexible hose, blast pipe and filtration storehouse lateral wall sliding connection, flexible hose's bottom has the exhaust column through shell fixedly communicate, the one end that the shell was kept away from to the exhaust column extends to inside the filtration storehouse, the shell rotation is connected with the fan, blast pipe outer wall fixedly connected with is located the outside connecting rod of filtration storehouse, the one end fixedly connected with bearing frame that the blast pipe was kept away from to the connecting rod, bearing frame and filtration storehouse sliding connection; the bearing seat is in threaded connection with a reciprocating screw rod, and the reciprocating screw rod is in rotary connection with the filtering bin;
the outside actuating mechanism that is equipped with of filtration storehouse, actuating mechanism can drive reciprocating screw and rotate and can drive the fan and rotate the convulsions, be equipped with the follow-up anti-blocking mechanism that is located the filter upside in the filtration storehouse.
As a further scheme of the invention, the follow-up anti-blocking mechanism comprises a casing and a wavy sliding groove, wherein the lower surface of the casing is attached to the upper surface of the filter plate, an air inlet groove aligned with the upper port of the flat air blowing nozzle is formed in the lower end of the casing, the inner wall of the casing is connected with an air collecting cover in a sliding manner in the up-down direction, exhaust grooves are symmetrically formed in the left side wall and the right side wall of the air collecting cover, injection channels are symmetrically formed in the left side wall and the right side wall of the casing, the upper port of the injection channels can be communicated with the exhaust grooves on the same side, the outer wall of the casing is fixedly connected with a sliding rod parallel with the air supply pipe, one end, far away from the casing, of the sliding rod extends out of the filter chamber and is fixedly connected with a connecting rod, the wavy sliding groove is formed in the inner wall of the filter chamber, the sliding rod is connected with a sliding block in a sliding manner, the sliding block is fixedly connected with the air collecting cover, and the upper side of the air collecting cover is connected with a passive stirring mechanism positioned on the upper side of the injection channels.
As a further scheme of the invention, the driving mechanism comprises a motor, the motor is fixedly connected with the outer wall of the filter bin, an output shaft of the motor is fixedly connected with the reciprocating screw rod, the output shaft of the motor is fixedly connected with a first bevel gear, the first bevel gear is meshed with a second bevel gear, the second bevel gear is rotationally connected with the outer wall of the filter bin, a rotating shaft of the second bevel gear is fixedly connected with a third bevel gear, the third bevel gear is meshed with a fourth bevel gear, the fourth bevel gear is rotationally connected with the outer wall of the filter bin, a rotating shaft of the fourth bevel gear is fixedly connected with a worm, the worm is meshed with a turbine ring, and the turbine ring is fixedly connected with a rotating shaft of the fan.
As a further scheme of the invention, the passive stirring mechanism comprises a first cross rod and a twisting rotating shaft, wherein the first cross rod and the twisting rotating shaft are parallel to the air supply pipe, the first cross rod is in sliding connection with the inner wall of the filtering bin in the up-down direction, the first cross rod is in sliding connection with a sliding sleeve, the sliding sleeve is fixedly connected with the gas collecting hood, the first cross rod is hinged with a push rod, the twisting rotating shaft is in rotating connection with the inner wall of the filtering bin, a plurality of stirring rods are fixedly connected to the circumferential wall of the twisting rotating shaft, the outer wall of the twisting part of the twisting rotating shaft is in rotating connection with a rotating sleeve, and the outer wall of the rotating sleeve is fixedly connected with the push rod.
As a further scheme of the invention, an ejection mechanism is arranged at the lower side of the flat blowing nozzle in the filter bin and comprises a second cross rod, the second cross rod is parallel to the air supply pipe and fixedly connected with the inner wall of the filter bin, the outer wall of the second cross rod is slidably connected with a moving block, the moving block is fixedly connected with a sleeve, the inner wall of the sleeve is slidably connected with a push rod, a spring is arranged between the lower end of the push rod and the inner bottom end of the sleeve, and the upper end of the push rod extends out of the sleeve and is fixedly connected with the bottom of the flat blowing nozzle.
As a further scheme of the invention, the upper end of the filter bin is provided with a sealing cover, the lower end of the filter bin is fixedly communicated with a funnel nozzle, and the lower end of the funnel nozzle is inserted with a collecting test tube.
A sorting and filtering device for tumor cell mass, which is characterized in that: the exhaust pipe stretches into the one end in the filtration storehouse and bends downwards, the loudspeaker cover has been cup jointed to the one end outer wall of exhaust pipe bending downwards.
A method of sorting and filtering a tumor cell mass, the method comprising the steps of:
step one: firstly, opening a sealing cover, pouring tissue fluid containing tumor cell clusters into the upper side of the filter plate in the filter bin, and then closing the sealing cover:
step two: then starting a driving mechanism, driving a fan to exhaust air from the lower side area of the filter plate in the filter bin by the driving mechanism, blowing away impurities blocked in the filter holes of the filter plate and at the upper port by using the pumped air from the flat air blowing nozzle, and simultaneously enabling the areas on the upper side and the lower side of the filter plate to form positive and negative pressure differences, thereby improving the filtering effect;
step three: the driving mechanism drives the follow-up anti-blocking mechanism simultaneously, so that the follow-up anti-blocking mechanism can collect and boost the gas blown by the flat blowing nozzle and jet the gas to the upper surface of the filter plate once every a period of time so as to blow away the accumulated matters which are closely attached to the upper port of the filter hole of the filter plate;
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism stirs tissue fluid on the upper side of the filter plate, and further impurities are prevented from accumulating;
step five: and finally, collecting the tumor cell mass after filtration and sorting by a collecting test tube, and taking down the collecting test tube after the filtration is finished so as to facilitate later-stage research and use.
Compared with the prior art, the invention has the beneficial effects that:
the invention can make the area of the lower side of the filter plate in the filter bin form negative pressure and make the area of the upper side of the filter plate in the filter bin form positive pressure, thereby accelerating the filtering speed of tumor cell mass in the mixed liquid of the upper side of the filter plate, and simultaneously repeatedly blowing out the sundries blocked in the filter holes of the filter plate upwards, thereby further improving the filtering speed of the tumor cell mass.
The follow-up anti-blocking mechanism can collect air blown by the flat blowing nozzle and form certain pressure, and can quickly spray accumulated air to the upper surface of the filter plate at intervals, and the air sprayed at high speed can blow away accumulated matters at the upper port of the filter hole of the filter plate, so that the filtering efficiency of the device is improved.
The follow-up anti-blocking mechanism can drive the passive stirring mechanism, so that the passive stirring mechanism can stir the mixed liquid on the upper side of the filter plate in the filter bin, and excessive impurities can be prevented from accumulating on the upper surface of the filter plate, thereby further improving the filtering efficiency of the filter.
Drawings
FIG. 1 is a schematic top view of the front side of the overall structure of the present invention;
FIG. 2 is a schematic view of the front side elevation of the overall structure of the present invention;
FIG. 3 is a schematic cross-sectional view of a filter cartridge and its internal structure;
FIG. 4 is an enlarged schematic view of a portion of FIG. 3A;
FIG. 5 is an enlarged schematic view of a portion of FIG. 3B;
FIG. 6 is an enlarged schematic view of a portion of FIG. 3 at C;
FIG. 7 is an enlarged schematic view of a portion of FIG. 3 at D;
FIG. 8 is a schematic cross-sectional view of a sleeve and its internal structure;
fig. 9 is a flow chart of the method of the present invention.
In the drawings, the list of components represented by the various numbers is as follows:
1. a filtering bin; 2. a filter plate; 3. a flat air blowing nozzle; 4. an air supply pipe; 5. a flexible hose; 6. an exhaust tube; 7. a connecting rod; 8. a bearing seat; 9. a reciprocating screw rod; 10. a casing; 11. an air inlet groove; 12. a gas collecting hood; 13. an exhaust groove; 14. a jet channel; 15. a slide bar; 16. wave-shaped sliding grooves; 17. a slide block; 18. a first cross bar; 19. a sliding sleeve; 20. a push rod; 21. twisting the rotating shaft; 22. a stirring rod; 23. a rotating sleeve; 24. a housing; 25. a vortex rim; 26. a fan; 27. a motor; 28. a first bevel gear; 29. a second bevel gear; 30. a third bevel gear; 31. a fourth bevel gear; 32. a worm; 33. a second cross bar; 34. a moving block; 35. a sleeve; 36. a push rod; 37. a spring; 38. sealing cover; 39. a hopper mouth; 40. collecting test tubes; 41. a horn cover.
Description of the embodiments
Referring to fig. 1-9, the present invention provides a technical solution: the utility model provides a sorting filter equipment of tumour cell group, includes filtration storehouse 1 and platykurtic air nozzle 3, middle part fixedly connected with filter 2 in the filtration storehouse 1, platykurtic air nozzle 3 is located filtration storehouse 1, and platykurtic air nozzle 3 upper port pastes with filter 2 lower surface, platykurtic air nozzle 3 fixed intercommunication has blast pipe 4, the one end that platykurtic air nozzle 3 was kept away from to blast pipe 4 extends to filtration storehouse 1 outside and is connected with flexible hose 5, blast pipe 4 and filtration storehouse 1 lateral wall sliding connection, flexible hose 5's bottom is through shell 24 fixed intercommunication having exhaust column 6, exhaust column 6 keeps away from the one end of shell 24 and extends to filtration storehouse 1 inside, shell 24 internal rotation is connected with fan 26, blast pipe 4 outer wall fixedly connected with is located the outside connecting rod 7 of filtration storehouse 1, the one end that blast pipe 4 was kept away from to the connecting rod 7 is fixedly connected with bearing frame 8, bearing frame 8 and filtration storehouse 1 sliding connection; the bearing seat 8 is in threaded connection with a reciprocating screw rod 9, and the reciprocating screw rod 9 is in rotary connection with the filter bin 1;
the outside of the filter bin 1 is provided with a driving mechanism, the driving mechanism can drive the reciprocating screw rod 9 to rotate and drive the fan 26 to rotate for exhausting, and the filter bin 1 is internally provided with a follow-up anti-blocking mechanism positioned on the upper side of the filter plate 2.
When the scheme is put into practical use, firstly, a proper amount of mixed liquid is poured into the upper side of the filter plate 2 in the filter bin 1, liquid with tumor cell clusters in the mixed liquid is slowly filtered downwards through the filter plate 2, then a driving mechanism is started, the driving mechanism drives a reciprocating screw rod 9 to rotate, the reciprocating screw rod 9 drives a bearing seat 8 to move left and right in a reciprocating manner, the bearing seat 8 drives a connecting rod 7 to move, the connecting rod 7 drives an air supply pipe 4 to move left and right, the air supply pipe 4 drives a flat air blowing nozzle 3 to move left and right, meanwhile, the driving mechanism drives a fan 26 to rotate, the fan 26 pumps air at the lower side of the filter plate 2 through an exhaust pipe 6, so that negative pressure is formed in the region of the lower side of the filter plate 2 in the filter bin 1, simultaneously, the pumped air is sequentially led to the telescopic hose 5, the air supply pipe 4 and the flat air blowing nozzle 3, and the blocking objects in the upper port of the filter plate 2 can be blown out after the flat upper port of the flat air blowing nozzle 3, and the blocking objects in the filter hole of the filter plate 2 can be formed, and the region at the upper side of the filter plate 2 can be positive pressure, so that the filter plate 2 in the filter bin 1 can be formed, the region of the filter clusters in the filter bin 2 can be further, and the tumor cell clusters can be formed in the region of the filter plate 2 in the filter bin 2, and the filter chamber can be repeatedly and the noise-up and the filter cell clusters can be increased.
As a further scheme of the invention, the follow-up anti-blocking mechanism comprises a casing 10 and a wavy sliding groove 16, wherein the lower surface of the casing 10 is attached to the upper surface of the filter plate 2, an air inlet groove 11 aligned with the upper port of the flat air blowing nozzle 3 is formed in the lower end of the casing 10, the inner wall of the casing 10 is connected with an air collecting cover 12 in a sliding manner in the up-down direction, air exhaust grooves 13 are symmetrically formed in the left side wall and the right side wall of the air collecting cover 12, an injection channel 14 is symmetrically formed in the left side wall and the right side wall of the casing 10, the upper port of the injection channel 14 can be communicated with the air exhaust grooves 13 on the same side, a sliding rod 15 parallel with the air blowing pipe 4 is fixedly connected to the outer wall of the casing 10, one end of the sliding rod 15, which is far away from the casing 10, extends out of the filter bin 1 and is fixedly connected with the connecting rod 7, the wavy sliding groove 16 is formed in the inner wall of the filter bin 1, a sliding block 17 is connected with the inner wall of the wavy sliding groove 16 in a sliding manner, the sliding block 17 is fixedly connected with the air collecting cover 12, and a passive stirring mechanism positioned on the upper side of the injection channel 14 is connected to the upper side of the air collecting cover 12.
When the scheme is put into practical use, when the connecting rod 7 reciprocates left and right, the connecting rod 7 drives the slide bar 15 to move simultaneously, the slide bar 15 drives the sleeve shell 10 to synchronously move with the flat blowing nozzle 3, air blown up by the flat blowing nozzle 3 passes through the filtering holes of the filter plate 2 and then can enter the gas collecting cover 12 through the gas inlet grooves 11, the gas collecting cover 12 can reciprocate left and right along with the sleeve shell 10, the gas collecting cover 12 can reciprocate up and down along the wavy sliding grooves 16 through the sliding blocks 17 while horizontally moving, when the gas collecting cover 12 ascends to the highest end area, the air outlet grooves 13 can be aligned with the upper ports of the spraying channels 14, before that, the air in the gas collecting cover 12 can be accumulated to form a certain pressure, after the air outlet grooves 13 can be aligned with the upper ports of the spraying channels 14, the air accumulated in the gas collecting cover 12 can be sprayed out to the upper surfaces of the rapid filter plates 2 through the spraying channels 14, and thus, the air blown up by the follow-up anti-up mechanism can collect the air blown up on the flat blowing nozzle 3 and form a certain pressure, the air accumulated on the upper surfaces of the rapid filter plates 2 can be sprayed out to the upper surfaces of the filter plates at certain intervals, and the filter plate can be sprayed out to the high-speed filter plate surface area after the filter plate can be closely adhered to the filter plate surface after the filter plate has high efficiency.
As a further scheme of the invention, the driving mechanism comprises a motor 27, the motor 27 is fixedly connected with the outer wall of the filter bin 1, an output shaft of the motor 27 is fixedly connected with a reciprocating screw rod 9, an output shaft of the motor 27 is fixedly connected with a first bevel gear 28, the first bevel gear 28 is meshed with a second bevel gear 29, the second bevel gear 29 is rotatably connected with the outer wall of the filter bin 1, a rotating shaft of the second bevel gear 29 is fixedly connected with a third bevel gear 30, the third bevel gear 30 is meshed with a fourth bevel gear 31, the fourth bevel gear 31 is rotatably connected with the outer wall of the filter bin 1, a rotating shaft of the fourth bevel gear 31 is fixedly connected with a worm 32, the worm 32 is meshed with a turbine ring 25, and the turbine ring 25 is fixedly connected with a rotating shaft of the fan 26; in operation, the motor 27 is started, the motor 27 drives the reciprocating screw rod 9 to rotate through the rotating shaft of the motor 27, the reciprocating screw rod 9 causes the flat blowing nozzle 3 and the casing 10 to synchronously reciprocate through a series of mechanical transmission, meanwhile, the motor 27 drives the first bevel gear 28 to rotate, the first bevel gear 28 drives the second bevel gear 29 to rotate, the second bevel gear 29 drives the third bevel gear 30 to rotate through the rotating shaft of the motor 29, the third bevel gear 30 drives the fourth bevel gear 31 to rotate, the fourth bevel gear 31 drives the worm 32 to rotate through the rotating shaft of the worm, and the worm 32 drives the vortex ring 25 to rotate, so that the fan 26 can be driven to perform exhausting work.
As a further scheme of the invention, the passive stirring mechanism comprises a first cross rod 18 and a twisting rotating shaft 21, wherein the first cross rod 18 and the twisting rotating shaft 21 are parallel to the air supply pipe 4, the first cross rod 18 is in sliding connection with the inner wall of the filter bin 1 in the up-down direction, the first cross rod 18 is in sliding connection with a sliding sleeve 19, the sliding sleeve 19 is fixedly connected with the gas collecting hood 12, the first cross rod 18 is hinged with a push rod 20, the twisting rotating shaft 21 is in rotating connection with the inner wall of the filter bin 1, a plurality of stirring rods 22 are fixedly connected to the circumferential wall of the twisting rotating shaft 21, a rotating sleeve 23 is rotatably connected to the outer wall of the twisting part of the twisting rotating shaft 21, and the outer wall of the rotating sleeve 23 is fixedly connected with the push rod 20.
When the scheme is put into practical use, when the gas collecting hood 12 reciprocates up and down, the gas collecting hood 12 can drive the first cross rod 18 to reciprocate up and down through the sliding sleeve 19, the first cross rod 18 drives the twisting rotating shaft 21 to rotate through the cooperation of the push rod 20 and the rotating sleeve 23, and the twisting rotating shaft 21 drives the stirring rods 22 to rotate, so that the follow-up anti-blocking mechanism can drive the passive stirring mechanism, the passive stirring mechanism can stir the mixed liquid on the upper side of the filter plate 2 in the filter bin 1, and excessive impurities can be prevented from being accumulated on the upper surface of the filter plate 2, and the filtering efficiency of the filter box is further improved.
As a further scheme of the invention, an ejection mechanism is arranged at the lower side of the flat blowing nozzle 3 in the filter bin 1, the ejection mechanism comprises a second cross rod 33, the second cross rod 33 is parallel to the air supply pipe 4 and is fixedly connected with the inner wall of the filter bin 1, a moving block 34 is slidably connected with the outer wall of the second cross rod 33, a sleeve 35 is fixedly connected with the moving block 34, an ejector rod 36 is slidably connected with the inner wall of the sleeve 35, a spring 37 is arranged between the lower end of the ejector rod 36 and the inner bottom end of the sleeve 35, and the upper end of the ejector rod 36 extends out of the sleeve 35 and is fixedly connected with the bottom of the flat blowing nozzle 3; in operation, by arranging the second cross bar 33 and the moving block 34, the spring 37 and the ejector rod 36 can reciprocate left and right along with the flat air nozzle 3; through the arrangement of the spring 37, the ejector rod 36 can always upwards prop against the flat air blowing nozzle 3, the upper port of the flat air blowing nozzle 3 can always cling to the lower surface of the filter plate 2, and leakage of air from a gap between the flat air blowing nozzle 3 and the filter plate 2 can be avoided.
The upper end of the filter bin 1 is provided with a sealing cover 38, the lower end of the filter bin 1 is fixedly communicated with a funnel mouth 39, and the lower end of the funnel mouth 39 is inserted with a collecting test tube 40; in operation, by means of the sealing cover 38, the mixed liquid can be conveniently poured into the filter house 1, and by means of the collecting test tube 40, the filtered tumor cell mass can be conveniently collected.
As a further scheme of the invention, one end of the exhaust pipe 6 extending into the filter bin 1 is bent downwards, and the outer wall of the bent end of the exhaust pipe 6 is fixedly sleeved with a horn cover 41; in operation, the horn cover 41 is provided to prevent liquid from being sucked into the suction pipe 6.
A method of sorting and filtering a tumor cell mass, the method comprising the steps of:
step one: firstly, opening a sealing cover 38, pouring tissue fluid containing tumor cell clusters into the filter cartridge 1 at the upper side of the filter plate 2, and then closing the sealing cover 38;
step two: then starting a driving mechanism, wherein the driving mechanism drives a fan 26 to exhaust air to the lower side area of the filter plate 2 in the filter bin 1, and the flat air blowing nozzle 3 blows away impurities blocked in the filter holes and at the upper port of the filter plate 2 by using the extracted air, and simultaneously, the areas on the upper side and the lower side of the filter plate 2 form positive and negative pressure differences, so that the filtering effect is improved;
step three: the driving mechanism drives the follow-up anti-blocking mechanism simultaneously, so that the follow-up anti-blocking mechanism can collect and boost the gas blown by the flat air blowing nozzle 3 and spray the gas to the upper surface of the filter plate 2 once every a period of time so as to blow away the accumulated matters which are closely attached to the upper port of the filter hole of the filter plate 2;
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism stirs tissue fluid on the upper side of the filter plate 2, and further impurities are prevented from accumulating;
step five: the tumor cell mass separated by filtration is finally collected by a collecting test tube 40, and after the filtration is completed, the collecting test tube 40 is taken down for later study.
Working principle: firstly, pouring a proper amount of mixed liquid into the upper side of the filter plate 2 in the filter bin 1, slowly filtering the mixed liquid with tumor cell clusters downwards through the filter plate 2, starting a driving mechanism, driving a reciprocating screw rod 9 to rotate, driving a bearing seat 8 to reciprocate left and right by the reciprocating screw rod 9, driving a connecting rod 7 to move by the bearing seat 8, driving an air supply pipe 4 to move by the connecting rod 7, driving a flat air blowing nozzle 3 to reciprocate left and right by the air supply pipe 4, simultaneously, driving a fan 26 to rotate, sucking air out of the lower side of the filter plate 2 by the fan 26 through an exhaust pipe 6, forming negative pressure in the region of the lower side of the filter plate 2 in the filter bin 1, simultaneously, leading the sucked air to a telescopic hose 5, the air supply pipe 4 and the flat air blowing nozzle 3 sequentially, blowing out to the filter plate 2 through the upper port of the flat air blowing nozzle 3, pressurizing the upper port of the flat air, and then blowing out the blocking objects in the filter holes of the filter plate 2 and the upper port, and forming positive pressure in the region of the upper side of the filter plate 2 in the filter bin 1, so that the region of the lower side of the filter plate 2 in the filter bin 1 forms negative pressure, and the tumor cell clusters in the filter bin 2 can be repeatedly blown out, and the speed of the tumor cell clusters in the filter bin 2 can be increased.
Claims (7)
1. A sorting and filtering device for tumor cell mass, which is characterized in that: including filter house (1) and platykurtic mouth (3) of blowing, middle part fixedly connected with filter (2) in filter house (1), platykurtic mouth (3) of blowing are located filter house (1), and platykurtic mouth (3) upper port pastes with filter (2) lower surface mutually, platykurtic mouth (3) of blowing fixedly communicate has blast pipe (4), the one end that blast pipe (4) kept away from platykurtic mouth (3) extends to outside filter house (1) and is connected with flexible hose (5), blast pipe (4) and filter house (1) lateral wall sliding connection, the bottom of flexible hose (5) is through shell (24) fixedly communicate with exhaust column (6), the one end that shell (24) was kept away from to exhaust column (6) extends to the below of filter (2), the inside rotation of shell (24) is connected with fan (26), the outer wall fixedly connected with of blast pipe (4) is located outside bearing frame (7) of filter house (1), the one end that blast pipe (7) kept away from blast pipe (8) is connected with (8) fixedly, filter house (1) and is slidingly connected with (8). The bearing seat (8) is in threaded connection with a reciprocating screw rod (9), and the reciprocating screw rod (9) is in rotary connection with the filter bin (1);
the outside of the filter bin (1) is provided with a driving mechanism which can drive the reciprocating screw rod (9) to rotate and can drive the fan (26) to rotate for exhausting air, and the filter bin (1) is internally provided with a follow-up anti-blocking mechanism positioned on the upper side of the filter plate (2);
the follow-up anti-blocking mechanism comprises a casing (10) and a wavy sliding groove (16), wherein the lower surface of the casing (10) is attached to the upper surface of a filter plate (2), an air inlet groove (11) aligned with the upper port of a flat air blowing nozzle (3) is formed in the lower end of the casing (10), an air collecting cover (12) is slidingly connected to the inner wall of the casing (10) in the up-down direction, an air exhaust groove (13) is symmetrically formed in the left side wall and the right side wall of the air collecting cover (12), an injection channel (14) is symmetrically formed in the left side wall and the right side wall of the casing (10), the upper port of the injection channel (14) can be communicated with the air exhaust groove (13) on the same side, a sliding rod (15) parallel to an air supply pipe (4) is fixedly connected to the outer wall of the casing (10), one end of the sliding rod (15) is far away from the outside of a filter bin (1) and is fixedly connected with a connecting rod (7), the wavy sliding groove (16) is formed in the inner wall of the filter bin (1), a sliding block (17) is slidingly connected to the inner wall of the air collecting cover (16), and the sliding block (17) is fixedly connected with the air collecting cover (12), and the air collecting mechanism is fixedly connected to the air collecting channel (12).
2. The sorting and filtering device for tumor cell mass according to claim 1, wherein: the driving mechanism comprises a motor (27), the motor (27) is fixedly connected with the outer wall of the filter bin (1), an output shaft of the motor (27) is fixedly connected with a reciprocating screw rod (9), the output shaft of the motor (27) is fixedly connected with a first bevel gear (28), the first bevel gear (28) is meshed with a second bevel gear (29), the second bevel gear (29) is rotationally connected with the outer wall of the filter bin (1), a rotating shaft of the second bevel gear (29) is fixedly connected with a third bevel gear (30), the third bevel gear (30) is meshed with a fourth bevel gear (31), the fourth bevel gear (31) is rotationally connected with the outer wall of the filter bin (1), a rotating shaft of the fourth bevel gear (31) is fixedly connected with a worm (32), the worm (32) is meshed with a turbine ring (25), and the turbine ring (25) is rotationally connected with a rotating shaft of the fan (26).
3. The sorting and filtering device for tumor cell mass according to claim 1, wherein: the passive stirring mechanism comprises a first cross rod (18) and a twisting rotating shaft (21), the first cross rod (18) and the twisting rotating shaft (21) are parallel to an air supply pipe (4), the first cross rod (18) is connected with the inner wall of a filtering bin (1) in a sliding mode in the up-down direction, the first cross rod (18) is connected with a sliding sleeve (19) in a sliding mode, the sliding sleeve (19) is fixedly connected with a gas collecting hood (12), the first cross rod (18) is hinged to a push rod (20), the twisting rotating shaft (21) is connected with the inner wall of the filtering bin (1) in a rotating mode, a plurality of stirring rods (22) are fixedly connected to the circumferential wall of the twisting rotating shaft (21), a rotating sleeve (23) is connected to the outer wall of the twisting portion of the twisting rotating shaft (21) in a rotating mode, and the outer wall of the rotating sleeve (23) is fixedly connected with the push rod (20).
4. The sorting and filtering device for tumor cell mass according to claim 1, wherein: the utility model discloses a filter cabinet, including filter cabinet (1), flat mouth (3) downside of blowing is equipped with ejection mechanism, ejection mechanism includes second horizontal pole (33), second horizontal pole (33) are parallel with blast pipe (4) and with filter cabinet (1) inner wall fixed connection, second horizontal pole (33) outer wall sliding connection has movable block (34), movable block (34) fixedly connected with sleeve pipe (35), sleeve pipe (35) inner wall sliding connection has ejector pin (36), be equipped with spring (37) between ejector pin (36) lower extreme and sleeve pipe (35) inner bottom, sleeve pipe (35) and with flat mouth (3) bottom fixed connection of blowing are extended to ejector pin (36) upper end.
5. The sorting and filtering device for tumor cell mass according to claim 1, wherein: the upper end of the filter bin (1) is provided with a sealing cover (38), the lower end of the filter bin (1) is fixedly communicated with a funnel mouth (39), and the lower end of the funnel mouth (39) is inserted with a collecting test tube (40).
6. The sorting and filtering device for tumor cell mass according to claim 1, wherein: one end of the exhaust pipe (6) extending into the filter bin (1) is bent downwards, and a horn cover (41) is fixedly sleeved on the outer wall of one end of the exhaust pipe (6) bent downwards.
7. A method for sorting and filtering a tumor cell mass, which is suitable for the apparatus for sorting and filtering a tumor cell mass according to claim 3, comprising the steps of:
step one: firstly, opening a sealing cover (38), pouring tissue fluid containing tumor cell clusters into the filter plate (2) at the upper side of the filter chamber (1), and then closing the sealing cover (38):
step two: then starting a driving mechanism, wherein the driving mechanism drives a fan (26) to exhaust air from the lower side area of the filter plate (2) in the filter bin (1), and the flat air blowing nozzle (3) blows away impurities blocked in the filter holes and at the upper ports of the filter plate (2) by using the pumped air, and simultaneously, the positive and negative pressure differences are formed in the areas on the upper side and the lower side of the filter plate (2), so that the filtering effect is improved;
step three: the driving mechanism drives the follow-up anti-blocking mechanism simultaneously, so that the follow-up anti-blocking mechanism can collect and boost the gas blown by the flat air blowing nozzle (3) and jet the gas to the upper surface of the filter plate (2) once every a period of time so as to blow away the accumulated matters which are closely attached to the upper port of the filter hole of the filter plate (2);
step four: the follow-up anti-blocking mechanism is linked with the passive stirring mechanism, so that the passive stirring mechanism stirs tissue fluid on the upper side of the filter plate (2) to further prevent impurities from accumulating;
step five: the tumor cell mass separated by filtration is finally collected by a collecting test tube (40), and after the filtration is completed, the collecting test tube (40) is taken down for later study.
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| CN114797215A (en) | 2022-07-29 |
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