CN110463823A - A kind of micro-ecological additive and preparation method thereof improving animal intestinal tract health - Google Patents
A kind of micro-ecological additive and preparation method thereof improving animal intestinal tract health Download PDFInfo
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- CN110463823A CN110463823A CN201811539069.6A CN201811539069A CN110463823A CN 110463823 A CN110463823 A CN 110463823A CN 201811539069 A CN201811539069 A CN 201811539069A CN 110463823 A CN110463823 A CN 110463823A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
Abstract
The invention discloses a kind of micro-ecological additives and preparation method thereof for improving animal intestinal tract health, which includes the component of following mass fraction: 2~5 parts of lactobacillus acidophilus, 2~5 parts of lactobacillus plantarum, 2~5 parts of enterococcus faecalis, 2~5 parts of Lactobacillus casei, 2~3 parts of Pediococcus acidilactici, 3 parts of bacillus subtilis, 2 parts of yeast culture, 2 parts of xylo-oligosaccharide, 3 parts of glucose.The present invention carries out scientific compatibility by screening efficient lactic acid bacterium, successfully develops the micro-ecological additive for improving animal intestinal tract health, can improve animal intestinal tract health, improve digestibility, promotes growth and breeding, improve economic benefit of aquaculture.
Description
Technical field
The present invention relates to a kind of micro-ecological additives and preparation method thereof for improving animal intestinal tract health.
Background technique
Feed cost accounts for absolute specific gravity in the breeding process, so relatively pay attention to the quality of feed in industry, and it is right
The understanding of nutrition also only resides in the level of feed；Disease to cultivation output have a significant impact, because in the industry to disease
Prevention and control are also paid much attention to, but more or are stopped in the treatment to control and prevention of disease, are prevented overweighting on slogan controlling, be controlled in the action big
Yu Fang.The decades of cultivation industry fast development, entire industry chain are also shooting up, and feed, vaccine and antibiotic etc. newly produce
Product emerge one after another, and technology is constantly updated, and the monitoring method and treatment means for coping with nutrition and disease are increasingly advanced, increasingly
Intelligence.
However, the domestic unavoidable reality of cultivation industry is that input and output is not directly proportional, epidemic situation becomes increasingly complex,
The disease the next refractory, and disease inherently will affect output, feeding management is not good at, abuse of antibiotics, treatment excessively etc. all can shadow
Growth and breeding is rung, is as a result exactly that output is reduced.High investment, the root of poor benefit on earth where answer is just in enteron aisle.
The quality that enteron aisle is digested and assimilated decides that body obtains the height of nutrition.Body nutrition decide immune system,
The health of the big physiological system of reproductive system, endocrine system, blood circulation system, nervous system etc. eight, and eight big physiological systems
Health just decide growth and breeding, finally determine the economic benefit of cultivation.Intestinal health decides body health, and
Gastrointestinal health is determined by the flora of gastrointestinal tract, if feeding management is not good at, environmental stress, disease, abuse of antibiotics etc.
Cause gastrointestinal bacterial flora unbalance, the probiotics of ratio dominance is substituted by harmful bacteria, harmful bacteria can generate a large amount of exotoxins,
Endotoxin and active chemical analysis first damage gastrointestinal tract mucosa layer, then destroy gastrointestinal tract epithelial cell, cause
" intestines leakage ", then cause intestine immunity reaction (intestinal inflammatory), malnutritive and disease is eventually led to, the warp of aquaculture is reduced
Ji benefit.
Summary of the invention
In order to overcome drawbacks described above, the present invention provides it is a kind of improve animal intestinal tract health micro-ecological additive, to
Improve animal intestinal tract health, improve digestibility, promote growth and breeding, increases economic efficiency.
The beneficial effects of the present invention are: the present invention carries out scientific compatibility by screening efficient functional probiotics strain,
Successfully develop improve animal intestinal tract health micro-ecological additive, can improve animal intestinal tract health, raising digestibility,
Promote growth and breeding, improves economic benefit of aquaculture.
Specific embodiment
With reference to embodiments, it elaborates to the present invention.But protection scope of the present invention is not limited to following implementations
Example all still belongs to this that is, in every case with simple equivalent changes and modifications made by scope of the present invention patent and description
Within patent of invention covering scope.
A kind of micro-ecological additive improving animal intestinal tract health, the component including following mass fraction: lactobacillus acidophilus
2~5 parts, it is 2~5 parts of lactobacillus plantarum, 2~5 parts of enterococcus faecalis, 2~5 parts of Lactobacillus casei, 2~3 parts of Pediococcus acidilactici, withered
3 parts of careless bacillus, 2 parts of yeast culture, 2 parts of xylo-oligosaccharide, 3 parts of glucose.
The preparation method of the micro-ecological additive of above-mentioned improvement animal intestinal tract health, comprising the following steps:
Step 1, matrix manufacturing is cultivated:
The production of A culture medium: firstly, by 2.5 parts of peptone, 4 parts of glucose, 0.5 part of yeast extract, 0.5 part of sodium acetate,
0.25 part of precipitated calcium carbonate, 0.4 part of Triammonium citrate, 0.2 part of tween, 0.015 part of dipotassium hydrogen phosphate, 0.03 part of magnesium sulfate,
0.01 part of manganese sulfate, 0.01 part of sodium chloride, 65~75 parts of distilled water are heated to 68~72 DEG C, and stirring makes each dissolution of raw material；So
Afterwards, with 20% sodium hydroxide (NaOH) solution tune pH value to 7.0~7.2, ingredient is completed；It is sterilized 20 points with 120~125 DEG C again
Zhong Hou, temperature are cooled to 37 DEG C, obtain the A culture medium of sterilizing；
The production of B culture medium: firstly, by 1.5 parts of peptone, 4 parts of glucose, 0.5 part of yeast extract, dipotassium hydrogen phosphate
0.02 part, 0.25 part of precipitated calcium carbonate, 0.01 part of sodium chloride, 65~75 parts of distilled water are heated to 70 DEG C or so, and stirring makes each
Dissolution of raw material；Then, with 20% sodium hydroxide (NaOH) solution tune pH value to 6.9~7.1, ingredient is completed；Again with 120~125
DEG C sterilizing 20 minutes, temperature is cooled to 37 DEG C, obtains the B culture medium of sterilizing；
Step 2, the activation and expansion culture of strain:
The A culture medium that the lactobacillus acidophilus of refrigeration is inoculated in the above-mentioned sterilizing of 100ml is once activated, temperature 36
It is detected after~38 DEG C, 30 hours, the A culture medium that 100ml bacterium solution is all accessed to the above-mentioned sterilizing of 500ml after detection is up to standard carries out
Re-activation, temperature be 36~38 DEG C, 25 hours after detect it is up to standard after, expanded culture in the A culture medium of above-mentioned sterilizing
72 hours；
The activation and expansion culture of lactobacillus plantarum are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of enterococcus faecalis are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of Lactobacillus casei are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of Pediococcus acidilactici are the same as above-mentioned lactobacillus acidophilus；
The B culture medium that the bacillus subtilis of refrigeration is inoculated in the above-mentioned sterilizing of 100ml is once activated, temperature is
Detected after 35~37 DEG C, 30 hours, detect it is up to standard after by 100ml bacterium solution all the access above-mentioned sterilizings of 500ml B culture mediums into
Row re-activation, temperature be 35~37 DEG C, 25 hours after detect it is up to standard after, carry out expansion training in the B culture medium of above-mentioned sterilizing
It supports 72 hours；
Step 3, the collection of bacterium mud:
The lactobacillus acidophilus liquid after fermentation is centrifuged with centrifuge, and collects bacterium mud；
The bacterium mud collection method of lactobacillus plantarum is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud collection method of enterococcus faecalis is the same as above-mentioned lactobacillus acidophilus；
The above-mentioned same lactobacillus acidophilus of the bacterium mud collection method of Lactobacillus casei；
The bacterium mud collection method of Pediococcus acidilactici is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud collection method of bacillus subtilis is the same as above-mentioned lactobacillus acidophilus；
Step 4, the freeze-drying of bacterium mud:
Freeze-drying preparation is carried out to be coated with bacterium powder treatment process for lactobacillus acidophilus mud collected by step 3, it is specific to walk
Suddenly are as follows: a. bacterium mud be added 10% ratio sucrose, the glucan of 10% ratio and the ethylene glycol of 2% ratio, mix, b. contain into
It freezes plate to freeze 50 minutes at -30 DEG C, c. is freezed 60 minutes at -80 DEG C, and d. is freeze-dried on frozen vacuum dryer
13 hours, in e. freeze-dried powder -4~-6 DEG C of refrigerators of closed preservation；
The bacterium mud freeze-drying method of lactobacillus plantarum is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of enterococcus faecalis is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of Lactobacillus casei is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of Pediococcus acidilactici is the same as above-mentioned lactobacillus acidophilus；
Bacillus subtilis bacterium mud collected by step 3 is subjected to spray drying preparation, specific steps are as follows: a. bacterium mud is added
15% beta-cyclodextrin mixes, and b. sets spray dryer parameter are as follows: and 90 DEG C of outlet temperature, 150 DEG C of inlet temperature, pan feeding speed
700ml/h is spent, carries out spray drying powder processed, in c. bacterium powder 4~6 DEG C of environment of closed preservation；
Step 5, prepared by finished product:
Take 2~5 parts of the lactobacillus acidophilus prepared in step 4,2~5 parts of lactobacillus plantarum, 2~5 parts of enterococcus faecalis, cheese
2~5 parts of lactobacillus, 2~3 parts of Pediococcus acidilactici, 3 parts of bacillus subtilis, 2 parts of yeast culture, 2 parts of xylo-oligosaccharide, Portugal
3 parts of grape sugar, packs after mixing under aseptic conditions, obtains a kind of micro-ecological additive for improving animal intestinal tract health.
Action principle of the invention are as follows: 1, probiotics stomach juice-resistant, the resistance to choline of micro-ecological additive of the present invention smoothly arrive
Up to after animal intestinal tract, probiotics can generate the digestive ferments such as exoprotein enzyme, amylase, lipase, help digest decomposition food
In nutrition, the various vitamins of synthesis, the absorption for promoting minerals and microelement, guarantee animal body abundance and balanced
Nutrition；2, the probiotics energy metabolism of micro-ecological additive of the present invention generates short chain fatty acids, reduces pH value, inhibits pathogen, thorn
Swash the differentiation of enteron aisle Crypt Stem Cells, repair gut epithelium, promote gut epithelium to secrete mucus, eliminate intestinal inflammatory, improves dynamic
Object immunity；3, the metabolism of the probiotics of micro-ecological additive of the present invention generate short chain fatty acids be the blind rectum epithelial cell of knot and
The energy source of intestine immunity cell can safeguard knot caecum normal stool, absorption and excretory function, improve intestine immunity water
It is flat；4, the prebiotics of micro-ecological additive of the present invention can promote the growth and breeding of intestinal beneficial bacterium.
1~3 the present invention will be described by the following examples.
Examples 1 to 3 is respectively according to composition of raw materials and technological parameter shown in table 1.
1. Examples 1 to 3 of table
Above-described embodiment 1~3 is subjected to field experiment, field experiment is respectively provided with experimental group, and (addition Tiny ecosystem of the present invention adds
Add agent) and control group (being not added with micro-ecological additive of the present invention).
1, experiment one: effect of the micro-ecological additive of the present invention to weanling pig
1) test site: Henan Zhumadian large―scale hoggery.
2) experimental design: 160 21 age in days weanling pigs of selection are randomly divided into tetra- groups of A, B, C, D, one group 40；A,B,
C, the D group original body mass that is averaged is respectively 6.82kg, 6.79kg, 6.88kg, 6.96kg；A group basal diet is to be added to 0.1%
Micro-ecological additive embodiment 1 of the present invention, B group basal diet be added to 0.1% micro-ecological additive of the present invention implement
Example 2, C group basal diet are the micro-ecological additive embodiment 3 of the present invention for being added to 0.1%, and D group is basic daily ration；A,B, C
Group is experimental group, and D group is control group.After feeding in 40 days, compare tetra- groups of daily gains of A, B, C, D, feedstuff-meat ratio and diarrhea rate.
3) experimental result is shown in Table 2 (data statistics of experiment one).By experimental data it is found that daily gain aspect A, B, component C
Not Wei 0.484kg, 0.479kg, 0.499 kg, much higher than the 0.409kg of D group；In terms of feedstuff-meat ratio A, B, component C be not 1.50,
1.54,1.48, lower than the 1.67 of D group；A, B, component C are not 3.0%, 4.0%, 3.0% in terms of diarrhea rate, far below D group
30.1%.
The data statistics of the experiment of table 2. one
2, experiment two: effect of the micro-ecological additive of the present invention to growing and fattening pigs
1) place: Henan Dengfeng large―scale hoggery is tested
2) experimental design: 160 70 age in days growing and fattening pigs of selection are randomly divided into tetra- groups of A, B, C, D, one group 40；A,B,C,
The D group original body mass that is averaged is respectively 24.72kg, 25.61kg, 25.36kg, 25.76kg；A group basal diet is to be added to
0.1% micro-ecological additive embodiment 1 of the present invention, B group basal diet are the Tiny ecosystem of the present invention addition for being added to 0.1%
Agent embodiment 2, C group basal diet are the micro-ecological additive embodiment 3 of the present invention for being added to 0.1%, and D group is basic daily ration；
A, B, C group are experimental group, and D group is control group.Feeding compares tetra- groups of daily gains of A, B, C, D and feedstuff-meat ratio to delivering for sale.
3) experimental result is shown in Table 3 (data statistics of experiment two).By experimental data it is found that daily gain aspect A, B, component C
Not Wei 0.796kg, 0.776kg, 0.805 kg, much higher than the 0.679kg of D group；In terms of feedstuff-meat ratio A, B, component C be not 2.14,
2.22,2.12, lower than the 2.52 of D group.
The data statistics of the experiment of table 3. two
3, experiment three: effect of the micro-ecological additive of the present invention to laying hen
1) place: Hubei Wuhan large size laying hen is tested
2) experimental design: random selection enters four of laying period with age in days, and number is A, B, C and D respectively
, laying hen is respectively 4950,4900,4970,4970；A group basal diet is the present invention for being added to 0.1%
Micro-ecological additive embodiment 1, B group basal diet are the micro-ecological additive embodiment 2 of the present invention for being added to 0.1%, C group
Basal diet is the micro-ecological additive embodiment 3 of the present invention for being added to 0.1%, and D group is basic daily ration；A, B, C group are real
Group is tested, D group is control group.Feeding 90 days is compared tetra- average egg weights of A, B, C, D, feed intake, feedstuff-egg ratio, laying rate, egg and is closed
Lattice rate and death and culling rate.
3) experimental result is shown in Table 4 (data statistics of experiment three).By experimental data it is found that average egg weight aspect A, B
, C be respectively 54.32g, 54.86g, 54.27g, be much higher than D 50.18g；A, B, C in terms of feedstuff-egg ratio
Respectively 2.35,2.33,2.35, hence it is evident that 2.54 lower than D；A in terms of laying rate, B, C be respectively 91.4%,
92.1%, 91.7%, hence it is evident that 86.2% higher than D；A in terms of egg qualification rate, B, C be respectively 99.7%,
99.8%, 99.7%, hence it is evident that 96.3% higher than D；A in terms of death and culling rate, B, C be respectively 0.08%, 0.07%,
0.08%, hence it is evident that 2.33% lower than D.
The data statistics of the experiment of table 4. three
4, experiment four: effect of the micro-ecological additive of the present invention to broiler chicken
1) place: Kaifeng large size broiler chicken field is tested
2) experimental design: selection is randomly divided into 4 groups, every group 10000, numbers respectively with 40000 broiler chicken seedlings of age in days
For A group, B group, C group and D group；A group basal diet is the micro-ecological additive embodiment 1 of the present invention for being added to 0.1%, B group
Basal diet is the micro-ecological additive embodiment 2 of the present invention for being added to 0.1%, and C group basal diet is to be added to 0.1%
Micro-ecological additive embodiment 3 of the present invention, D group are basic daily ration；A, B, C group are experimental group, and D group is control group.Feeding is extremely
It delivers for sale, compares A, B, C, D tetra- groups of survival rates, feedstuff-meat ratios, delivers counterpoise, expenses for medicine service condition for sale.
3) experimental result is shown in Table 5 (data statistics of experiment four).By experimental data it is found that delivering quantitative aspects A group, B for sale
Group, component C are not 9752,9802,9789, and D group is 9460；A group, B group, component C are not in terms of survival rate
97.5%, 98%, 97.9%, D group is 94.7%；Deliver for sale counterpoise A group, B group, C group be respectively 2.30kg, 2.31kg,
2.33kg, D group are 2.03kg；In terms of feedstuff-meat ratio A group, B group, component C be not 1.94,1.93,1.93, D group be 2.12；Expenses for medicine
Use aspect A group, B group, component C are not 5040 yuan, 5220 yuan, 5400 yuan, and D group is 11240 yuan.
The data statistics of the experiment of table 5. four
5, experiment conclusion
By four experimental datas of embodiment 1, embodiment 2 and embodiment 3 it can be seen that the growth and breeding of experimental group
And situations such as economic benefit, is significantly better than control group.
In conclusion micro-ecological additive of the present invention is improving animal intestinal tract health, is improving digestibility, promotion life
Long breeding, aspect of increasing economic efficiency significant effect.
Claims (2)
1. a kind of micro-ecological additive for improving animal intestinal tract health, which is characterized in that the component including following mass fraction: thermophilic
2~5 parts of Lactobacillus lactis, 2~5 parts of lactobacillus plantarum, 2~5 parts of enterococcus faecalis, 2~5 parts of Lactobacillus casei, Pediococcus acidilactici 2~
3 parts, 3 parts of bacillus subtilis, 2 parts of yeast culture, 2 parts of xylo-oligosaccharide, 3 parts of glucose.
2. a kind of preparation method of the micro-ecological additive as described in claim 1 for improving animal intestinal tract health, feature exist
In, comprising the following steps:
Step 1, matrix manufacturing is cultivated:
The production of A culture medium: firstly, by 2.5 parts of peptone, 4 parts of glucose, 0.5 part of yeast extract, 0.5 part of sodium acetate, lightweight carbon
0.25 part of sour calcium, 0.4 part of Triammonium citrate, 0.2 part of tween, 0.015 part of dipotassium hydrogen phosphate, 0.03 part of magnesium sulfate, manganese sulfate
0.01 part, 0.01 part of sodium chloride, 65~75 parts of distilled water are heated to 68~72 DEG C, and stirring makes each dissolution of raw material；Then, with
20% sodium hydroxide (NaOH) solution tune pH value completes ingredient to 7.0~7.2；Again after twenty minutes with 120~125 DEG C of sterilizings, temperature
Degree is cooled to 37 DEG C, obtains the A culture medium of sterilizing；
The production of B culture medium: firstly, by 1.5 parts of peptone, 4 parts of glucose, 0.5 part of yeast extract, 0.02 part of dipotassium hydrogen phosphate,
0.25 part of precipitated calcium carbonate, 0.01 part of sodium chloride, 65~75 parts of distilled water are heated to 70 DEG C or so, and stirring makes each dissolution of raw material；
Then, with 20% sodium hydroxide (NaOH) solution tune pH value to 6.9~7.1, ingredient is completed；It is sterilized 20 points with 120~125 DEG C again
Clock, temperature are cooled to 37 DEG C, obtain the B culture medium of sterilizing；
Step 2, the activation and expansion culture of strain:
The A culture medium that the lactobacillus acidophilus of refrigeration is inoculated in the above-mentioned sterilizing of 100ml is once activated, temperature is 36~38
DEG C, it is detected after 30 hours, the A culture medium that 100ml bacterium solution is all accessed to the above-mentioned sterilizing of 500ml after detection is up to standard carries out secondary work
Change, temperature be 36~38 DEG C, 25 hours after detect it is up to standard after, expanded culture 72 hours in the A culture medium of above-mentioned sterilizing；
The activation and expansion culture of lactobacillus plantarum are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of enterococcus faecalis are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of Lactobacillus casei are the same as above-mentioned lactobacillus acidophilus；
The activation and expansion culture of Pediococcus acidilactici are the same as above-mentioned lactobacillus acidophilus；
The B culture medium that the bacillus subtilis of refrigeration is inoculated in the above-mentioned sterilizing of 100ml is once activated, temperature is 35~37
DEG C, it is detected after 30 hours, the B culture medium that 100ml bacterium solution is all accessed to the above-mentioned sterilizing of 500ml after detection is up to standard carries out secondary work
Change, temperature be 35~37 DEG C, 25 hours after detect it is up to standard after, expanded culture 72 hours in the B culture medium of above-mentioned sterilizing；
Step 3, the collection of bacterium mud:
The lactobacillus acidophilus liquid after fermentation is centrifuged with centrifuge, and collects bacterium mud；
The bacterium mud collection method of lactobacillus plantarum is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud collection method of enterococcus faecalis is the same as above-mentioned lactobacillus acidophilus；
The above-mentioned same lactobacillus acidophilus of the bacterium mud collection method of Lactobacillus casei；
The bacterium mud collection method of Pediococcus acidilactici is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud collection method of bacillus subtilis is the same as above-mentioned lactobacillus acidophilus；
Step 4, the freeze-drying of bacterium mud:
Freeze-drying preparation, specific steps are carried out to be coated with bacterium powder treatment process for lactobacillus acidophilus mud collected by step 3 are as follows:
A. sucrose, the glucan of 10% ratio and the ethylene glycol of 2% ratio of 10% ratio is added in bacterium mud, mixes, and b. contains flat into freezing
Ware freezes 50 minutes at -30 DEG C, and c. is freezed 60 minutes at -80 DEG C, and d. is freeze-dried 13 hours on frozen vacuum dryer, e.
In freeze-dried powder -4~-6 DEG C of refrigerators of closed preservation；
The bacterium mud freeze-drying method of lactobacillus plantarum is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of enterococcus faecalis is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of Lactobacillus casei is the same as above-mentioned lactobacillus acidophilus；
The bacterium mud freeze-drying method of Pediococcus acidilactici is the same as above-mentioned lactobacillus acidophilus；
Bacillus subtilis bacterium mud collected by step 3 is subjected to spray drying preparation, specific steps are as follows: a. bacterium mud is added 15%
Beta-cyclodextrin mixes, and b. sets spray dryer parameter are as follows: and 90 DEG C of outlet temperature, 150 DEG C of inlet temperature, pan feeding speed
700ml/h, carries out spray drying powder processed, in c. bacterium powder 4~6 DEG C of environment of closed preservation；
Step 5, prepared by finished product:
Take 2~5 parts of the lactobacillus acidophilus prepared in step 4,2~5 parts of lactobacillus plantarum, 2~5 parts of enterococcus faecalis, cheese cream bar
2~5 parts of bacterium, 2~3 parts of Pediococcus acidilactici, 3 parts of bacillus subtilis, 2 parts of yeast culture, 2 parts of xylo-oligosaccharide, glucose 3
Part, it is packed after mixing under aseptic conditions, obtains a kind of micro-ecological additive for improving animal intestinal tract health.
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| CN112493366A (en) * | 2020-12-07 | 2021-03-16 | 北京市农林科学院 | Compound microbial inoculum for solid-state fermentation weaned piglet feed, preparation method and application |
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