CN110420128A - A kind of dark hair hair-growing and anti-hair loss composition and shampoo - Google Patents

A kind of dark hair hair-growing and anti-hair loss composition and shampoo Download PDF

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CN110420128A
CN110420128A CN201910718251.6A CN201910718251A CN110420128A CN 110420128 A CN110420128 A CN 110420128A CN 201910718251 A CN201910718251 A CN 201910718251A CN 110420128 A CN110420128 A CN 110420128A
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hair
cinnamic acid
amide
composition
shampoo
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林宜凡
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A61K8/447—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof containing sulfur
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/02—Preparations for cleaning the hair
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists

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  • Pharmacology & Pharmacy (AREA)
  • Cosmetics (AREA)

Abstract

The present invention provides a kind of dark hair hair-growing and anti-hair loss composition and shampoo, the composition includes cinnamic acid amino acid amide and/or its salt as effective component, and cinnamic acid amino acid amide is cinnamic acid methionine amide and/or cinnamic acid ala amide and/or cinnamic acid threonine amide.The present invention also provides the Hair-loss-preventingshampoo shampoos comprising the composition.Composition of the invention can effectively inhibit 5α-reductase active, inhibit testosterone to convert to protona, can slow down or treat androgenetic alopecia；Scalp blood circulation can also be improved, boosted metabolism, accelerate Hair growth rate, extend growth period and increase hair follicle number, promote the growth of hair, be effectively improved alopecia symptom；It can also promote hair follicle stem cells proliferation, differentiation by regulation Wnt/p-catenin signal path, and then realize the facilitation to hair growth, for alopecia, the withered, white hair of hair etc., hair can be enabled pitch-black beautiful, it is submissive bright and clean, reduce alopecia, white hair.

Description

A kind of dark hair hair-growing and anti-hair loss composition and shampoo
Technical field
The invention belongs to shampoo technical fields, and in particular to a kind of dark hair hair-growing and anti-hair loss composition and shampoo.
Background technique
The soil that scalp is depended on for existence as hair, the balance of ecological environment are the basic of hair healthy growth.Scalp It is most thick one of the skin of human body, blood vessel rich in；The speed of its aging is 6 times of facial skin, is body skin skin 12 times；Scalp area is about 650~700cm2, average follicle population is 200~250/cm2(sebaceous glands on scalp is close Degree is about 144~192/cm2, that on forehead is about 52~79/cm2, it is about 42~78/cm on cheek)；Head The sebum amount of epidermis face secretion is in 12h up to 288 μ g/cm2, and the sebum amount of forehead only has 144 μ g/cm2.Each hair has It is the mutually different period, de- after the growth of growth stage (anagen), catagen (catagen) and stand-down (telogen) It falls.The phenomenon that alopecia is epilation is divided into physiological and pathological.Physiologic alopecia finger hair normally falls off.One The hair of adult has 100,000 or so, and generally falling five or six ten hairs daily is normal phenomenon, and having spent 50 years old can also increase Daily 150 are added to, hair layer can be more and more thinner.Pathologic alopecia is a kind of hair follicle disease that clinic is common, according to incomplete system Meter, hair loss patient account for about the 20%~30% of adult population；Though serious disease is not belonging to, to the spirit and psychology of people Health generates strong influence.It is a danger signal that the daily hair loss of young man, which is more than 100, if such case continues two or three A month, and the hair front fallen down is sharp (nature growth cycle is not arrived in expression, belongs to abnormal fall), just very It may be that alopecia arrives.As social pressures constantly increase and the accelerating rhythm of life, the continuous deterioration of environment, with And undesirable eating habit, also growing day by day with our non-health, inferior health, hair loss patient is more and more.So effectively Anticreep development hair product be daily use chemicals circle current and future important research direction, the effect of objectively evaluating daily chemical products has Significance.
Summary of the invention
It is an object of the present invention to provide one kind comprising cinnamic acid amino acid amide and/or its salt as effective component Dark hair hair-growing and anti-hair loss composition.
It is another object of the present invention to provide a kind of dark hair hair tonic and hair losing preventings of above-mentioned dark hair hair-growing and anti-hair loss composition Send out shampoo.
The technical solution that the present invention is taken to achieve the above object are as follows: dark hair hair-growing and anti-hair loss composition includes cortex cinnamomi Sour amino acid amide and/or its salt are as effective component.
Preferably, cinnamic acid amino acid amide is cinnamic acid methionine amide and/or cinnamic acid ala amide and/or meat Cinnamic acid threonine amide.
Above-mentioned dark hair hair-growing and anti-hair loss composition is preparing the purposes in Anti-hair loss cosmetic composition.
Preferably, cosmetic composition is selected from by hair lotion, profit hair-cream, daily hair treatment, hair lotion, hair nutrition Water, shampoo, hair conditioner, treatment wax, hair-cream, nutrient hair cream, hair moisturiser, hair massage cream, pomade, hair spray, Hair film, nutrient hair mask, hair soap, shining shampoo milk, hair oil, trichoxerosis agent, hair antiseptic treating agent, hair dye, agent for permanent hair waving, hair Send out decolorising agent, hair jelly, hair glaze, hair cosmetic agent, hair lacquer, hair moisturizer, mousse and surely hair agent composition group in dosage form.
A kind of dark hair hair-growing and anti-hair loss shampoo includes above-mentioned dark hair hair-growing and anti-hair loss composition.
Preferably, shampoo includes the cinnamic acid amino acid amide of 0.1-10 μM of concentration.
Compared with prior art, the invention has the benefit that dark hair hair-growing and anti-hair loss composition of the present invention can be effective Inhibit 5 α-reductase activity, so that testosterone be inhibited to convert to protona, can slow down or treat androgenetic alopecia；It can also change Kind scalp blood circulation, boosts metabolism, and accelerates Hair growth rate, extends growth period and increase hair follicle number, to promote Into the growth of hair, it is effectively improved alopecia symptom；Furthermore it can also promote hair cream by regulation Wnt/p-catenin signal path The proliferation of capitulum, differentiation, and then realize the facilitation to hair growth；Therefore, contain dark hair hair-growing and anti-hair loss of the present invention The shampoo of composition has effects that prevent hair loss and trichogenous.Be used for a long time can also nourishing hair, root of hair and Scalp can be enabled hair pitch-black beautiful, submissive bright and clean, promote hair tonic, reduce de- for alopecia, the problems such as hair is withered, white hair Hair, white hair.
Present invention employs above-mentioned technical proposals to provide dark hair hair-growing and anti-hair loss composition and shampoo, compensates for existing skill The deficiency of art, design rationally, make and easy to use.
Detailed description of the invention
Fig. 1 is the content of phenolic substances in plant extract in test example 1 of the present invention；
Fig. 2 is the inhibiting rate to 5α-reductase of plant extract in test example 1 of the present invention；
Fig. 3 is cationic sesbania gum degree of substitution in test example 2 of the present invention；
Fig. 4 is cationic sesbania gum viscosity in test example 2 of the present invention；
Fig. 5 is cationic sesbania gum uv absorption property in test example 1 of the present invention；
Fig. 6 is shampoo cardability test result in test example 1 of the present invention；
Fig. 7 be in test example 1 of the present invention Hair-loss-preventingshampoo shampoo to hair growth effect schematic diagram.
Specific embodiment
Hereinafter, the present invention will be described in detail.
" alopecia " means that the phenomenon that hair falls off from scalp or hair are thinning less or the state that attenuates in this specification, institute Calling " preventing hair loss " means to prevent and inhibit depilation phenomenon as described above, and so-called " promoting hair growth " means not only Promote the generation of new hair, also grows previous hair health.
Present embodiment provides a kind of dark hair hair-growing and anti-hair loss composition, includes cinnamic acid amino acid amide and/or its salt As effective component.Above-mentioned salt is that salt is added in the acid formed by pharmaceutically acceptable free acid, can as above-mentioned free acid Use organic acid and inorganic acid.Above-mentioned organic acid does not limit to and this, but includes acetic acid, malic acid, citric acid, lactic acid, winestone Acid, maleic acid, fumaric acid, formic acid, propionic acid, oxalic acid, trifluoroacetic acid, benzoic acid, gluconic acid, methanesulfonic acid, glycolic, succinic acid, P-methyl benzenesulfonic acid, glutamic acid and aspartic acid.Also, above-mentioned inorganic acid is not limited to this, but includes hydrochloric acid, bromic acid, sulfuric acid And phosphoric acid.
Shown in above-mentioned cinnamic acid amino acid amide such as formula (I):
Formula (I), synthetic route are as follows:
Specific synthetic method are as follows:
Caffeic acid and organic solvent are placed in reaction vessel, condensing agent and amino acid are added under the conditions of 0 DEG C, then 0 1-3h is reacted at DEG C, 5-8h is reacted under normal temperature condition, reaction solution is concentrated under reduced pressure after reaction, and second is added in concentrate Acetoacetic ester extraction, which shakes up, is put into refrigerator overnight, is extracted with ethyl acetate after reduced pressure three times, organic phase successively uses 3-5% lemon Lemon acid solution, the washing of saturated lemon sodium water solution, organic layer anhydrous Na2SO4It is dry, be concentrated under reduced pressure product is chromatographed through column Purify to obtain cinnamic acid amino acid amide.Cinnamic acid amino acid amide has no toxic side effect, and can take orally.
Wherein, organic solvent is selected from triethylamine and/or tetrahydrofuran；Condensing agent be selected from DCC/DMAP, HATU/NMM, EDC/HOBt/NMM or CDMT/NMM.
Above-mentioned cinnamic acid amino acid amide is cinnamic acid methionine amide shown in formula (II), cinnamic acid third shown in formula (III) Cinnamic acid threonine amide shown in propylhomoserin amide, formula (IV)；
Above-mentioned dark hair hair-growing and anti-hair loss composition is preparing the purposes in Anti-hair loss cosmetic composition.Cosmetic composition For selected from by hair lotion, profit hair-cream, daily hair treatment, hair lotion, hair nutrition water, shampoo, hair conditioner, treatment wax, hair Cream, nutrient hair cream, hair moisturiser, hair massage cream, pomade, hair spray, hair film, nutrient hair mask, hair soap, hair washing Milk, hair oil, trichoxerosis agent, hair antiseptic treating agent, hair dye, agent for permanent hair waving, hair decolorising agent, hair jelly, hair glaze, hairdressing Agent, hair lacquer, hair moisturizer, mousse and surely hair agent composition group in dosage form.
Present embodiment also provides a kind of dark hair hair-growing and anti-hair loss shampoo, combines comprising above-mentioned dark hair hair-growing and anti-hair loss Object.
Above-mentioned shampoo includes the cinnamic acid amino acid amide of 0.1-10 μM of concentration.But the concentration can be according to the journey of alopecia It is suitably changed in during degree, the age of patient, weight, health status, gender, administration routes and treatment etc..
Above-mentioned shampoo does not limit to and this, also comprising natural plant extracts, conditioner, surfactant, pH adjusting agent, Metal-chelator assigns rouge agent.Wherein, natural plant extracts are selected from the linseed, fleece-flower root, olive leaf, Radix Angelicae Sinensis, pea, black One or more of mixing of beans, corn, Apple Seeds, pears seed, pine needle, cacumen biotae, Radix Astragali, radix scutellariae；Preferably the fleece-flower root, Radix Angelicae Sinensis, The mixing of semen sojae atricolor, pine needle, cacumen biotae, radix scutellariae, its ratio be 50:30:30:20:20:15；Conditioner be cationic cellulose, Cation guar gum, cationic sesbania gum or silicone emulsion；Surfactant be selected from glyceryl oleate, cocoyl glucoside, Cocoamidopropyl betaine, sodium lauroyl sarcosine, certain herbaceous plants with big flowers base glucoside, PEG-80 anhydrous sorbitol laurate, methyl coconut palm One or more of oleoyl taurate, C14-16 alkene sulfonic acid sodium, coconut oleoyl amine methyl MEA mixing；PH adjusting agent is Sodium citrate, citric acid, phosphoric acid and tartaric acid；Metal-chelator is disodium ethylene diamine tetraacetate and ethylenediamine tetra-acetic acid；Assign rouge Agent is mixed selected from one or more of almond glyceride type, PEG-7 glyceryl cocoate, PPG-3 Octyl Ether.
It is next by the following examples that the present invention is furture elucidated.However, it should be understood that the embodiment is merely illustrative Purpose, be not intended to limit scope and spirit of the present invention.
Embodiment 1:
The Inhibitory Effects of the cinnamic acid amino acid amide to 5α-reductase
The preparation method of rat 5α-reductase
Cleaning grade female sd inbred rats 3 are taken, cervical dislocation is put to death after being deprived of food but not water overnight, is taken out and is shredded on liver ice platform. 1:5 homogenate is made with the buffer of pre-cooling and glass homogenizer, is centrifuged 15min under the conditions of 4 DEG C, 10000rpm, takes endochylema portion It is centrifuged 1h under the conditions of point 100000rpm, outwells supernatant, precipitating is thick particulate extract.Certain volume buffer is added, and Homogenate suspension is carried out with homogenizer.Packing, -80 DEG C of refrigerators save.
Blank control group: 1.5mL 5α-reductase reaction system are as follows: the addition concentration of 0.5mL 5α-reductase preparation solution For 4.0mg/mL；20 μM of phosphate buffer solutions of 0.5mL pH6.5；150 μ L 0.5mg/mL testosterones；250μL 2.0mg/mL NADPH。
Test 1 group: 1.5mL 5α-reductase reaction system are as follows: the addition concentration of 0.5mL 5α-reductase preparation solution is 4.0mg/mL；20 μM of phosphate buffer solutions of 0.5mL pH6.5；150 μ L 0.5mg/mL testosterones；250μL 2.0mg/mL NADPH；3.0 μM of 0.1mL of cinnamic acid methionine amide.
Test 2 groups: 1.5mL 5α-reductase reaction system are as follows: the addition concentration of 0.5mL 5α-reductase preparation solution is 4.0mg/mL；20 μM of phosphate buffer solutions of 0.5mL pH6.5；150 μ L 0.5mg/mL testosterones；250μL 2.0mg/mL NADPH；3.0 μM of 0.1mL of cinnamic acid ala amide.
Test 3 groups: 1.5mL 5α-reductase reaction system are as follows: the addition concentration of 0.5mL 5α-reductase preparation solution is 4.0mg/mL；20 μM of phosphate buffer solutions of 0.5mL pH6.5；150 μ L 0.5mg/mL testosterones；250μL 2.0mg/mL NADPH；3.0 μM of 0.1mL of cinnamic acid threonine amide.
The measurement of blank control group: absorbance change value △ A is surveyed after 37 DEG C of constant-temperature incubation 30min0。
The measurement of experimental group and Finasteride group: 37 DEG C of incubations react 30min, respective absorbance are measured at 340nm Changing value △ An, the size of its inhibiting rate is calculated according to formula, test is repeated 3 times, and is averaged.
Calculation formula are as follows: I (%)=(△ A0-ΔAn)/△A0× 100%.
Measurement result is as shown in Figure 1, it can be seen that the present invention tests 1 group, tests 2 groups and tests 3 groups to 5α-reductase Inhibiting rate be above 85%, this illustrate cinnamic acid amino acid amide of the present invention to the inhibiting effect of 5α-reductase, so as to Enough testosterone is inhibited to convert to protona, and then slows down or treat androgenetic alopecia.
Embodiment 2:
Cinnamic acid amino acid amide is on the sanguimotor influence of scalp
The growth and regeneration of hair depend on enough blood supply of the body to hair follicle.Hair follicle is surrounded by vescular bed abundant, When microcirculatory perfusion obstacle, velocity of blood flow declines in microcirculation, influences normal supply of the body to nutrition needed for hair follicle, leads Hair follicle is caused to lack nutrient, to alopecia occur.So investigating influence of the cinnamic acid amino acid amide to mouse skin temperature, visitor It sees and reacts its influence to skin microcirculation, have great importance.
Specifically, cleaning grade mouse 40 are taken, half male and half female, weight (18 ± 2g).Back wool is cut short, is smeared at its back 6% vulcanized sodium depilatory agent, depilation area are 3cm × 3cm, after 3min with warm water cleaning (it is smooth with back of mice, without hurting nothing Subject to residual hair).Mouse is randomly divided into 4 groups by next day, and each 10,75% ethyl alcohol (is applied to each group by respectively blank control group Mouse hair removal section), 75% ethyl alcohol containing 3.0 μM of cinnamic acid amino acid amides (is applied to mouse hair removal section) by test group, each group Dosage is shown in Table 1.It smears 1 time, the skin temperature and different time points of each group mouse before measurement experiment is distinguished with infrared radiation thermometer (5,15,30,45,60,90min) skin temperature.Laboratory temperature is 19~21 DEG C.As a result as shown in Fig. 2, administration 5min and After 15min, due to containing a certain amount of ethyl alcohol in medical fluid, ethyl alcohol, which volatilizees, takes away the heat of skin, therefore groups of animals skin The equal decrease to some degree of temperature；Compared with blank group, test group mouse skin temperature decreases, but reduces amplitude Respectively less than blank group, this illustrates that cinnamic acid amino acid amide can reduce skin temperature caused by ethyl alcohol volatilizees and reduce.Administration After 30min, the skin temperature of blank group still is below level before administration；Compared with blank group, test group mouse skin temperature is equal Horizontal higher than before experiment, this shows that cinnamic acid amino acid amide can improve mouse skin temperature, to promote blood circulation.It gives After medicine 45min, the skin temperature of blank group still is below level before administration；Compared with blank group, test group mouse skin temperature It is preceding horizontal to be above experiment.After 60 min are administered, the skin temperature of blank group still is below level before administration, but becomes in rising Gesture；Compared with blank group, test group mouse skin temperature is still above level before experiment.After 90min is administered, the skin of blank group Skin temperature still is below level before administration, but in rising trend；Compared with blank group, test group mouse skin temperature is still high It is horizontal before experiment.In conclusion cinnamic acid amino acid amide, which has, increases mouse skin temperature, expansion blood vessel, kind microcirculation Effect, further relate to cinnamic acid amino acid amide can expand skin heart, improve skin microcirculation, increase papilla blood Supply improves hair follicle nutrition, to promote hair growth.
The applying amount and sample number of mouse between the different groups of table 1
Embodiment 3:
Influence of the cinnamic acid amino acid amide to hair growth
Take cleaning grade mouse 40, half male and half female, weight (18 ± 2g).Back wool is cut short, smears 6% sulphur at its back Change sodium depilatory agent, depilation area is 3cm × 3cm, and warm water cleaning is used after 3min, and (smooth with back of mice, no wound is without residual hair It is quasi-).Mouse is randomly divided into 4 groups by next day, and each 10,75% ethyl alcohol (is applied to each group mouse to take off by respectively blank control group Hair-fields), 75% ethyl alcohol containing 3.0 μM of cinnamic acid amino acid amides (is applied to mouse hair removal section, wherein test 1 by test group Group is cinnamic acid methionine amide group；2 groups are tested as cinnamic acid ala amide group, 3 groups of test are cinnamic acid threonine amide Group), each group is smeared 2 times daily, and each 0.1mL continuously smears 21d.
1. the measurement of hair length
Since back of mice grows hair, every the hair length of survey in 1 day, until off-test.Each group mouse takes 3 A epilation position, each position are pulled off the feather of 10, and pulled off the feather of hair vernier caliper measurement is denoted as with the distance that hair both ends are farthest Hair length, as a result such as Fig. 3.From figure 3, it can be seen that cinnamic acid amino acid amide has facilitation to hair growth.
2. om observation and the counting of mouse hair follicles
The 10th, 20 day cervical dislocation is put to death after mouse depilation respectively.Each each group respectively takes 5 mouse, in epilation portion Bit parallel is drawn materials in vertebra, and the hair newly grown is cut off, then skin is cut into the strip of 1cm wide, be affixed on hardboard On, 24-36h is fixed with 10% formalin, tap water washes away formalin, gradient alcohol dehydration, and paraffin embedding repairs block, cuts Piece makes slice direction consistent with the y direction of hair growth, and slice thickness is 6 μm, hematoxylin eosin staining, optical microphotograph The histological change of microscopic observation mouse skin hair follicle is counted and is analyzed, as a result such as Fig. 4.Figure 4, it is seen that meat Cinnamic acid amino acid amide can increase hair follicle number, to promote the growth of hair, be effectively improved alopecia symptom.
Embodiment 4:
Influence of the cinnamic acid amino acid amide to human hair papilla cell cultivation effect and signaling molecule
Mesoderm origin fibroblast of the hair papilla cell as the basal part for being located at hair follicle, passes through the hair base with hair follicle Interaction between cell plastid plays an important role in the growth of hair.Cinnamic acid ammonia is investigated using hair papilla cell Whether base acid amide has effects that hair growth.
1. cinnamic acid amino acid amide is to human hair papilla cell cultivation effect
Human hair papilla cell is inoculated in 50ml culture bottle by the density of 30-50 papilla of every culture bottle, at 37 DEG C Temperature, 5% CO2It is cultivated in incubator.After most of papilla is adherent, change liquid processing, while cannot be adherent or still The fibr tissue and other impurities of not adherent papilla, ball top portion remove, and are pure papilla in culture bottle.To cell After merging about 70-80%, cell culture fluid is discarded, with 0.25% trypsase by cell dissociation to suspended state, then is added rapidly Enter the DMEM culture medium containing 0% fetal calf serum to terminate digestion, 1000rpm is centrifuged 5min, abandons supernatant, is added in culture bottle new Cell is resuspended in the fresh DMEM culture medium 4mL for containing 10% fetal calf serum, piping and druming, then passes on, is inoculated in culture bottle by 1:3 37 DEG C of temperature, 5% CO2It is cultivated in incubator, it is seen that DPC moves out radial growth after papilla adherent 2 days, collects for the 3rd generation Cell.
The 3rd generation hair papilla cell of collection is made into individual cells suspension using CCK8 detection cell Proliferation, with every hole 1 ×104A cell inoculation is to 96 orifice plates, every 200 μ l of hole.(5 multiple holes of setting, edge hole are filled with sterile PBS)；Orifice plate is set It is incubated in incubator, overnight, after adherent, sucks culture solution, 200 μ of cinnamic acid amino acid amide of 3.0 μM of gradients is added in test group (1 group of test is cinnamic acid methionine amide group to L；2 groups are tested as cinnamic acid ala amide group, 3 groups of test are revived for cinnamic acid Propylhomoserin amide group), the culture solution of equal quantities is added in blank group, continues to cultivate 72h.Then, 20 μ l of CCK8 is added in every hole, continues It is placed in incubator and is incubated for 4h, then detected with microplate reader, select 450nm wavelength, measuring and recording as a result, finding out each examination The value of the mean light absorbency of sample group relatively analyzes proliferation degree, as a result as shown in Figure 5 compared with the absorbance value of blank group.From As can be seen that cinnamic acid amino acid amide can promote the proliferation of hair papilla cell in Fig. 5, there is trichogenous function Effect.
2. the influence that cinnamic acid amino acid amide expresses human hair papilla cell secrete cytokines and signaling molecule
Hair papilla cell can secrete cytokine profiles (such as stem cell factor (SCF), vascular endothelial cell growth because Son (VEGF), transforming growth factor β (TGF-β), hepatocyte growth factor (HGF), keratinocyte growth factor, pancreas islet Plain like growth factor (IGF) etc.) and signaling molecule (such as Wnt, noggin, BMP2/4) stimulation epithelial cell proliferation, differentiation, Starting hair follicle enters growth period.In order to detect whether cinnamic acid amino acid amide generates the expression of Wnt/ β-catenin signal It influences, we detect the expression of these genes using RT-PCR technology.
Single cell suspension is made by passage step in 3rd generation cell of collection, by 1 × 106It is seeded in 6 orifice plates, 37 DEG C temperature, 5% CO2Incubator is incubated for 80% cell monolayer and is paved with bottom hole.Then the operation of liquid kit is extracted by Trizol Step extracts cell total rna, then carries out reverse transcription to RNA, obtains corresponding cDNA, then carry out qRT-PCR reaction, primer Sequence such as table 2, as a result as shown in Figure 6.Wnt10b, Lef-1 and c-myc expression up-regulation as the result is shown, and to HGF, IGF-1 and The expression of TGF-β 2 is without obvious effect.These are the result shows that cinnamic acid amino acid amide has raised the secretion of Wnt10b, energy Wnt/ β-catenin signal path is enough activated, and can be expressed by hair papilla cell Lef-1, c-myc and transfer to enhance Wnt/ The activity and promotion cell differentiation of β-catenin signal path, to promote hair follicle formation and cyclical growth.
2 qRT-PCR primer sequence of table
Embodiment 5:
A kind of dark hair hair-growing and anti-hair loss shampoo includes 3 μM of cinnamic acid methionine amides, 6% extractive of flax seed, 3% Apple seed extract, 8% pears seed extract, 12% Semen sojae atricolor extract, 7% corn extract, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl glucoside, 21% Cocoamidopropyl betaine, 3% certain herbaceous plants with big flowers base glucose Glycosides, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% citric acid, 0.5% disodium ethylene diamine tetraacetate, 0.1% PEG-60 almond glycerol Esters, 3% PEG-7 glyceryl cocoate.
Embodiment 6:
A kind of dark hair hair-growing and anti-hair loss shampoo, comprising 4 μM of cinnamic acid ala amides, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl glucoside, 21% Cocoamidopropyl betaine, 3% certain herbaceous plants with big flowers base glucose Glycosides, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% citric acid, 0.5% disodium ethylene diamine tetraacetate, 0.1% PEG-60 almond glycerol Esters, 3% PEG-7 glyceryl cocoate.
Embodiment 7:
A kind of dark hair hair-growing and anti-hair loss shampoo, comprising 0.8 μM of cinnamic acid threonine amide, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl glucoside, 21% Cocoamidopropyl betaine, 3% certain herbaceous plants with big flowers base glucose Glycosides, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% citric acid, 0.5% ethylenediamine tetra-acetic acid, 0.1% PEG-60 almond glyceride type, 3% PEG-7 glyceryl cocoate.
Embodiment 8:
A kind of dark hair hair-growing and anti-hair loss shampoo includes 0.1 μM of cinnamic acid methionine amide, 0.2 μM of cinnamic acid threonine Amide, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl glucoside, 21% coconut oleoyl amine Propyl betaine, 3% certain herbaceous plants with big flowers base glucoside, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% citric acid, 0.5% ethylenediamine tetra-acetic acid, 0.1% PEG-60 almond glyceride type, 3% PEG-7 glyceryl cocoate.
Embodiment 9:
A kind of dark hair hair-growing and anti-hair loss shampoo, comprising 4 μM of cinnamic acid methionine amides, 3 μM of cinnamic acid ala amides, 3 μM of cinnamic acid threonine amides, 6% extractive of flax seed, 3% apple seed extract, 8% pears seed extract, 12% semen sojae atricolor mention Take object, 7% corn extract, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl glucoside, 21% Cocoamidopropyl betaine, 3% certain herbaceous plants with big flowers base glucoside, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% citric acid, 0.5% Disodium ethylene diamine tetraacetate, 0.1% PEG-60 almond glyceride type, 3% PEG-7 glyceryl cocoate.
Embodiment 10:
A kind of dark hair hair-growing and anti-hair loss shampoo includes 0.5 μM of cinnamic acid methionine amide, 1 μM of cinnamic acid alanine acyl Amine, 2 μM of cinnamic acid threonine amides, 10% radix-polygoni multiflori extract, 5% Olive leaf P.E, 3% Semen sojae atricolor extract, 17% sides CedarLeaves extract, 7% corn extract, 0.3% cation guar gum, 0.5% glyceryl oleate, 1.2% cocoyl Portugal Glucosides, 21% Cocoamidopropyl betaine, 3% certain herbaceous plants with big flowers base glucoside, 3% sodium methyl cocoyl taurate, 2.5% C14-16 alkene sulfonic acid sodium, 1.8% coconut oleoyl amine methyl MEA, 1.2% sodium citrate, 0.5% lemon Acid, 0.5% disodium ethylene diamine tetraacetate, 0.1% PEG-60 almond glyceride type, 3% PEG-7 glyceryl cocoate.
Test example 1:
Evaluation of the dark hair hair-growing and anti-hair loss shampoo to hair growth effect
Take cleaning grade mouse 60, half male and half female, weight (18 ± 2g).Back wool is cut short, smears 6% sulphur at its back Change sodium depilatory agent, depilation area is 3cm × 3cm, and warm water cleaning is used after 3min, and (smooth with back of mice, no wound is without residual hair It is quasi-).Mouse is randomly divided into 6 groups by next day, each 10, cleans embodiment 5-10 Hair-loss-preventingshampoo shampoo lose hair or feathers in mouse respectively Area, 2 times a day, each 1mL are continuous to smear 2 months, take the photograph photo, and compare hair growth effect, as a result respectively such as Fig. 7 and table 3 Shown (wherein * * * * * is represented very good, and * * * * is represented, and * * * is represented commonly, and it is poor that * * is represented, and * represents excessively poor).From Fig. 7 With table 3 as can be seen that embodiment 5-10 Hair-loss-preventingshampoo shampoo has extraordinary hair growth effect.
Evaluation of 3 Hair-loss-preventingshampoo shampoo of table to hair growth effect
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail It repeats.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.

Claims (6)

1. a kind of Anti-hair loss composition, it is characterised in that: comprising cinnamic acid amino acid amide and/or its salt as effective component.
2. Anti-hair loss composition according to claim 1, it is characterised in that: the cinnamic acid amino acid amide is cinnamic acid Methionine amide and/or cinnamic acid ala amide and/or cinnamic acid threonine amide.
3. Anti-hair loss composition of any of claims 1 or 2 is preparing the purposes in Anti-hair loss cosmetic composition.
4. purposes according to claim 3, it is characterised in that: the cosmetic composition is selected from by hair lotion, profit hair Cream, daily hair treatment, hair lotion, hair nutrition water, shampoo, hair conditioner, treatment wax, hair-cream, nutrient hair cream, hair moisturizing Frost, hair massage cream, pomade, hair spray, hair film, nutrient hair mask, hair soap, shining shampoo milk, hair oil, trichoxerosis agent, hair Hair antiseptic treating agent, agent for permanent hair waving, hair decolorising agent, hair jelly, hair glaze, hair cosmetic agent, hair lacquer, hair moisturizer, rubs at hair dye Dosage form in silk and the surely group of hair agent composition.
5. a kind of Hair-loss-preventingshampoo shampoo, it is characterised in that: include Anti-hair loss composition of any of claims 1 or 2.
6. a kind of Hair-loss-preventingshampoo shampoo according to claim 5, it is characterised in that: the shampoo includes 0.1-10 μM The cinnamic acid amino acid amide of concentration.
CN201910718251.6A 2019-08-05 2019-08-05 A kind of dark hair hair-growing and anti-hair loss composition and shampoo Pending CN110420128A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114712256A (en) * 2022-05-07 2022-07-08 杭州梵歌生物科技有限公司 Stable moisturizing amino acid shampoo

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114712256A (en) * 2022-05-07 2022-07-08 杭州梵歌生物科技有限公司 Stable moisturizing amino acid shampoo
CN114712256B (en) * 2022-05-07 2023-08-11 杭州梵歌生物科技有限公司 Stable moisturizing amino acid shampoo

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