CN110404059A - A kind of functional thrombolysis enzyme preparation and preparation method thereof improving sleep - Google Patents
A kind of functional thrombolysis enzyme preparation and preparation method thereof improving sleep Download PDFInfo
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Abstract
The invention discloses a kind of functional thrombolysis enzyme preparations for improving sleep, ingredient containing following parts by weight: 1-15 parts of thrombolysis enzyme powder, 5-10 parts of bacillus subtilis powder, 20-35 parts of wild jujube powder, 15-20 parts of cassia seed powder, 15-25 parts of Rhizoma Gastrodiae powder, 15-25 parts of the fleece-flower root, 15-25 parts of chrysanthemum powder, 3-5 parts of licorice powder.Wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae have the function of certain promotion melatonin secretion, by being combined with the thrombolysis enzyme powder with thrombus effect, human body pineal body secretion melatonin can be effectively facilitated, so as to improve sleep quality of human body.The invention also discloses a kind of preparation methods of functional thrombolysis enzyme preparation for improving sleep.
Description
Technical field
The invention belongs to medicines and health protection technical fields, and in particular to it is a kind of improve sleep functional thrombolysis enzyme preparation and its
Preparation method.
Background technique
Pineal body is located at the top of diencephalon, is rich in blood vessel, and blood flow is only second to kidney.Pineal body can secrete take off it is black swash
Element can influence " biological clock " of human body, and then adjust sleep.Melatonin can improve sleep, and shortening is slept the preceding awakening time and entered
It sleeps the time, improves sleep quality, reduce number of awakening in sleep, shorten and shallowly sleep phases-time, extend the deep sleep stages time.So
And with advancing age, gradually atrophy ultimately causes the decrease of biological clock rhythmicity or even disappears pineal body up to calcification
It loses.After human body 35 years old, with pineal atrophy, the epiphysin of itself secretion is decreased obviously in vivo, is reduced within average every 10 years
10-15% leads to sleep disordered and a series of functional disturbance, and it is mankind's cerebral senility that melatonin levels, which reduce, sleep is reduced
Old one of the important signs that.
Currently, to improve sleep, the common method for maintaining melatonin level in middle-aged and the old's body is that direct supplement takes off
Black hormone, but there is apparent side effects for the melatonin because external source is used for a long time, and such as cause hypothermia, the excessive lactation of release
Hormone leads to infertile, the also side effect of reduction male's sexual desire；Or all kinds of Insomnia therapy drug such as hypnotics are taken, but because of medicine
Object abuse, can cause a series of side effects, such as drowsy, dizzy, bitter taste, nausea and amnesia to patient.Therefore, if can activate
Pineal body restores pineal body to the secreting function of melatonin, the intracorporal melatonin levels of machine can be made to maintain younger state,
Adjustment and recovery circadian rhythm, can not only so deepen to sleep, improve sleep quality, it is often more important that improve the machine of entire body
Energy state, improves the quality of living, the process of anti-aging, while avoiding the possible side effect of supplemented with exogenous melatonin.
Summary of the invention
It is an object of the invention to develop it is a kind of can effective human activin aging atrophy pineal body, it is living to restore pineal body
Power promotes the thrombolysis enzyme preparation and preparation method thereof of the improvement sleep of pineal body secretion melatonin.
In order to solve the above-mentioned technical problem, the technical scheme is that
A kind of functional thrombolysis enzyme preparation improving sleep, the ingredient containing following parts by weight: 1-15 parts of thrombolysis enzyme powder, withered
5-10 parts of careless bacillus bacterium powder, 20-35 parts of wild jujube powder, 15-20 parts of cassia seed powder, 15-25 parts of Rhizoma Gastrodiae powder, fleece-flower root 15-25
Part, 15-25 parts of chrysanthemum powder, 3-5 parts of licorice powder.
Preferably, a kind of functional thrombolysis enzyme preparation improving sleep, the ingredient containing following parts by weight: thrombolysis enzyme powder 5-
15 parts, 5-10 parts of bacillus subtilis powder, 25-35 parts of wild jujube powder, 15-20 parts of cassia seed powder, 15-25 parts of Rhizoma Gastrodiae powder, the fleece-flower root
20-25 parts of powder, 15-25 parts of chrysanthemum powder, 3-5 parts of licorice powder.
Preferably, a kind of functional thrombolysis enzyme preparation improving sleep, the ingredient containing following parts by weight: thrombolysis enzyme powder 15
Part, 5 parts of bacillus subtilis powder, 30 parts of wild jujube powder, 20 parts of cassia seed powder, 15 parts of Rhizoma Gastrodiae powder, 25 parts of polygonum multiflorum powder, chrysanthemum powder 15
Part, 3 parts of licorice powder.
Preferably, streptokinase powder purity is at least 90% in the functional thrombolysis enzyme preparation of preparation.
Preferably, the functional thrombolysis enzyme preparation of preparation is in powder, tablet, granule, decoction, oral solution and paste
Any one.
The present invention also provides a kind of preparation method of functional thrombolysis enzyme preparation for improving sleep, the thrombolysis enzyme preparations
For tablet, comprising the following steps:
Step 1, raw material crushes: wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is standby
With；
Step 2, it is sieved: the powder raw material in step 1 being crossed into 80-100 mesh screen, for two wheat-middlings of intransitable progress
It is broken to sieve for subsequent use up to all；
Step 3, thrombolysis enzyme powder is prepared: strain Subtilisin-QK ferments 12 at 25~35 DEG C~for 24 hours, take fermentation liquid
Supernatant purifying, freeze-drying, it is spare；
Step 4, it prepares bacillus subtilis powder: the sediment of fermentation liquid in step 3 being taken to wash, be lyophilized, it is spare；
Step 5, mixing, tabletting: wild jujube powder, cassia seed powder, Rhizoma Gastrodiae powder, He Shou prepared by step 2, step 3, step 4
Wu Fen, chrysanthemum powder, licorice powder, thrombolysis enzyme powder and bacillus subtilis powder ratio by weight mix, and tabletting improves sleep to obtain the final product
Functional streptokinase tablet.
Preferably, in the step 3 strain Subtilisin-QK further include before fermentation pancreas egg peptone culture medium preparation,
Strain Subtilisin-QK is placed in the pancreas egg peptone culture medium and is fermented.
Preferably, the pancreas egg peptone culture medium the preparation method comprises the following steps: take 10~20g of tryptone, phytone 2~
8g, 3~7g of sodium chloride add water to 1000ml, and it is spare that PH is adjusted to 7.0~7.5,121 DEG C of 10~20min of sterilizing.
Preferably, the supernatant of streptokinase fermentation liquid is at least 4mol/L ammonium sulfate with concentration in the step 3
It saltouts purifying.
Functionality thrombolysis enzyme preparation prepared by the present invention can be applied to improve the preparation of the drug or health care product of sleep.
Compared with prior art, the technology of the present invention effect is mainly reflected in:
(1) streptokinase has thrombus effect by what bacillus subtilis Subtilisin-QK fermentation generated to be a kind of
Protease, which can absorb by oral administration, and then improve microcirculation in human body.For oneself through the pineal body of atrophy occurs, lead to
Thrombus is crossed, the capillary of dredge blockage can make its recovery blood supply, improve atrophing state, improve or even restore to take off black sharp
The secretion capacity of element.Through experimental results demonstrate streptokinase is safe and reliable, can take for a long time, no obvious adverse reaction.
(2) thrombolysis enzyme powder, bacillus subtilis powder and wild jujube powder, cassia seed powder, Rhizoma Gastrodiae powder, polygonum multiflorum powder, inulin, Radix Glycyrrhizae
Powder is pressed than mixing, does not generate bad compatibility.
(3) wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root have the function of certain promotion melatonin secretion, inulin and Radix Glycyrrhizae
Powder has the function of clearing heat and removing internal heat, removing toxic substances, by being combined with the thrombolysis enzyme powder with thrombus effect, can be effectively improved people
The sleep quality of body.
The made functional thrombolysis enzyme preparation of this law has a better effect regular insomnia, has adjusting nerve with common
Excitatoty Insomnia therapy drug is compared, and this law products obtained therefrom is to improve pineal body atrophy and calcium by restoring pineal body blood supply
Change state promotes pineal body to improve or restore the secretion capacity to melatonin, and then adjusts human body rhythm and sleep state, changes
Kind wakefulness state.With conventional tranquillizer class treatment insomnia hypnotic compared with, this law products made thereby will not make human body generate it is drowsy,
The side effects such as giddy, bitter taste, nausea and amnesia；It takes for a long time simultaneously, also has and delay senescence, dark hair black hair and other effects.
Detailed description of the invention
Fig. 1 is the functional streptokinase tablet for the different doses of verifying that the embodiment of the present invention 8 provides to mice sleep shape
State impact effect figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
The strain Subtilisin-QK that the present invention uses oneself be stored in national Type Tissue Collection, bacterium numbering
For CCTCCNO:M203078.
Embodiment 1
The embodiment of the present invention 1 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 12g, phytone 2.5g are taken, sodium chloride 4g adds water to 1000ml, with sodium hydroxide and
PH is adjusted to 7.2 by hydrochloric acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is standby by 10~12min
With；Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferment 15h at 25 DEG C, and fermentation liquid is taken to exist
It is centrifuged 5min under 10000r/min and obtains the supernatant containing streptokinase, it will be molten in supernatant with the ammonium sulfate of 4mol/L
Bolt enzyme is gradually saltoutd purifying, and the thrombolysis enzyme powder for being lyophilized to obtain purity 92% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 250g that prepared by step 2, step 3, step 4, cassia seed powder 180g, Rhizoma Gastrodiae powder 210g, what
Radix polygoni multiflori powder 150g, chrysanthemum powder 250g, licorice powder 30g, thrombolysis enzyme powder 80g and bacillus subtilis powder 70g ratio by weight mix,
Tabletting is up to functional streptokinase tablet.
Embodiment 2
The embodiment of the present invention 2 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 15g, phytone 4g are taken, sodium chloride 5g adds water to 1000ml, with sodium hydroxide and salt
PH is adjusted to 7.3 by acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is spare by 12~15min；
Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferments at 25 DEG C for 24 hours, takes fermentation liquid in 10000r/
It is centrifuged 5min under min and obtains the supernatant containing streptokinase, with the ammonium sulfate of 4.5mol/L by the streptokinase in supernatant
It gradually saltouts purifying, the thrombolysis enzyme powder for being lyophilized to obtain purity 93% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 350g that prepared by step 2, step 3, step 4, cassia seed powder 150g, Rhizoma Gastrodiae powder 250g, what
Radix polygoni multiflori powder 250g, chrysanthemum powder 250g, licorice powder 50g, thrombolysis enzyme powder 100g and bacillus subtilis powder 50g ratio by weight are mixed
Even, tabletting is up to functional streptokinase tablet.
Embodiment 3
The embodiment of the present invention 3 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 18g, phytone 7g are taken, sodium chloride 6g adds water to 1000ml, with sodium hydroxide and salt
PH is adjusted to 7.3 by acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is spare by 15~20min；
Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferment 20h at 30 DEG C, takes fermentation liquid in 10000r/
It is centrifuged 5min under min and obtains the supernatant containing streptokinase, with the ammonium sulfate of 4.5mol/L by the streptokinase in supernatant
It gradually saltouts purifying, the thrombolysis enzyme powder for being lyophilized to obtain purity 92% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 300g that prepared by step 2, step 3, step 4, cassia seed powder 200g, Rhizoma Gastrodiae powder 150g, what
Radix polygoni multiflori powder 250g, chrysanthemum powder 150g, licorice powder 30g, thrombolysis enzyme powder 150g and 5 0g of bacillus subtilis powder ratio by weight are mixed
Even, tabletting is up to functional streptokinase tablet.
Embodiment 4
The embodiment of the present invention 4 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 15g, phytone 6g are taken, sodium chloride 5g adds water to 1000ml, with sodium hydroxide and salt
PH is adjusted to 7.4 by acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is spare by 15~20min；
Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferment 18h at 35 DEG C, takes fermentation liquid in 10000r/
Be centrifuged 5min under min and obtain the supernatant containing streptokinase, with the ammonium sulfate of 5mol/L by the streptokinase in supernatant by
Purifying of saltouing is walked, the thrombolysis enzyme powder for being lyophilized to obtain purity 91% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 200g that prepared by step 2, step 3, step 4, cassia seed powder 150g, Rhizoma Gastrodiae powder 200g, what
Radix polygoni multiflori powder 200g, chrysanthemum powder 200g, licorice powder 30g, thrombolysis enzyme powder 10g and bacillus subtilis powder 80g ratio by weight mix,
Tabletting is up to functional streptokinase tablet.
Embodiment 5
The embodiment of the present invention 5 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 20g, phytone 8g are taken, sodium chloride 7g adds water to 1000ml, with sodium hydroxide and salt
PH is adjusted to 7.3 by acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is spare by 15~20min；
Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferments at 25 DEG C for 24 hours, takes fermentation liquid in 10000r/
Be centrifuged 5min under min and obtain the supernatant containing streptokinase, with the ammonium sulfate of 4mol/L by the streptokinase in supernatant by
Purifying of saltouing is walked, the thrombolysis enzyme powder for being lyophilized to obtain purity 93% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 200g that prepared by step 2, step 3, step 4, cassia seed powder 200g, Rhizoma Gastrodiae powder 200g, what
Radix polygoni multiflori powder 200g, chrysanthemum powder 200g, licorice powder 30g, thrombolysis enzyme powder 30g and bacillus subtilis powder 80g ratio by weight mix,
Tabletting is up to functional streptokinase tablet.
Embodiment 6
The embodiment of the present invention 6 provides a kind of functional streptokinase tablet and preparation method thereof for improving sleep, including following
Step:
Step 1, dry wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, tryptone 15g, phytone 6g are taken, sodium chloride 5g adds water to 1000ml, with sodium hydroxide and salt
PH is adjusted to 7.2 by acid solution, is put into 121 DEG C of high-pressure sterilizing pots later and is sterilized that pancreas egg peptone culture medium is made is spare by 15~20min；
Strain Subtilisin-QK is put in above-mentioned pancreas egg peptone culture medium, ferments at 30 DEG C for 24 hours, takes fermentation liquid in 10000r/
It is centrifuged 5min under min and obtains the supernatant containing streptokinase, with the ammonium sulfate of 4.5mol/L by the streptokinase in supernatant
It gradually saltouts purifying, the thrombolysis enzyme powder for being lyophilized to obtain purity 92% for streptokinase after purification is spare；
Step 4, it takes the sediment of fermentation liquid in step 3 to be dissolved with distilled water to be centrifuged, as follows cleans and be lyophilized afterwards three times repeatedly
It is spare；
Step 5, the wild jujube powder 300g that prepared by step 2, step 3, step 4, cassia seed powder 150g, Rhizoma Gastrodiae powder 150g, what
Radix polygoni multiflori powder 150g, chrysanthemum powder 150g, licorice powder 50g, thrombolysis enzyme powder 50g and bacillus subtilis powder 100g ratio by weight are mixed
Even, tabletting is up to functional streptokinase tablet.
Embodiment 7
The embodiment of the present invention 7 provides functional thrombolysis enzyme prepared by 1~embodiment of a kind of verifying embodiment of the present invention 6
Agent is improving the method in sleep effect.
70 sleep disordered model mices are divided into 7 groups, number consecutively 1,2,3,4,5,6,7,1~6 group of number
The functional streptokinase tablet 4g prepared in 1~embodiment of above-described embodiment 6 is taken in stomach-filling to mouse respectively, and the 7th group of mouse then fills
Stomach takes 4g clear water as reference examples 7, and daily stomach-filling is taken once, and continuous gavage one week.When 7 groups of mouse are at sleep state
When, with 40 decibels of sound interferences sleep mouse 5s, the quantity for mouse of waking up is observed and recorded, the results are shown in Table 1:
Each ingredient number represents the number that each ingredient mixes by weight in each embodiment in table；
Mouse is hiding by day and coming out at night property animal, rest on general daytime, evening events, generally in the morning 8-9 point or so start into
It sleeps, afternoon, 5-6 point came into play.When position movement is not occurring for mouse, simple eye or eyes closure is regarded as falling asleep, and works as eyes
Position movement is opened or occurred, that is, regards as waking up.
Table 1
Data can see from table 1, functional streptokinase tablet prepared by 1~embodiment of the embodiment of the present invention 6
To be effectively improved the sleep disordered situation of mouse, mouse can be effectively facilitated and preferably slept.
Embodiment 8
The embodiment of the present invention 8 provides functional streptokinase tablet difference clothes prepared by a kind of verifying embodiment of the present invention 3
Dosage is improving the method in sleep effect.
60 sleep disordered model mices are divided into 3 groups, number consecutively 1,2,3, the mouse that 1,2 group of number fills respectively
Stomach takes functional streptokinase tablet 2g, the 4g prepared in above-described embodiment 3, and then 4g clear water is taken in stomach-filling to the 3rd group of mouse, daily
Stomach-filling is taken once, and continuous gavage after a week, observes and records mouse daytime sleepiness state, as a result as shown in Figure 1.
As shown in Figure 1, compared to control group mice, the sleep of the 1st, 2 group of mouse of einnehmen function streptokinase tablet and
Wake-up time obviously shifts to an earlier date, and activity mouse quantity of waking up halfway significantly reduces, and shows functional thrombolysis prepared by the present invention
Enzyme agent can be effectively improved mice sleep state；And the 2nd group of mouse teaches the sleep of the 1st group of mouse and wake-up time to shift to an earlier date, table
How many pairs of mice sleep situations of bright functionality streptokinase tablet usage amount have a significant impact.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with scope of protection of the claims
Subject to.
Claims (10)
1. a kind of functional thrombolysis enzyme preparation for improving sleep, which is characterized in that the ingredient containing following parts by weight: thrombolysis enzyme powder
1-15 parts, 5-10 parts of bacillus subtilis powder, 20-35 parts of wild jujube powder, 15-20 parts of cassia seed powder, 15-25 parts of Rhizoma Gastrodiae powder, He Shou
Black powder 15-25 parts, 15-25 parts of chrysanthemum powder, 3-5 parts of licorice powder.
2. a kind of functional thrombolysis enzyme preparation for improving sleep as described in claim 1, which is characterized in that contain following weight
The ingredient of part: 5-15 parts of thrombolysis enzyme powder, 5-10 parts of bacillus subtilis powder, 25-35 parts of wild jujube powder, 15-20 parts of cassia seed powder, day
Numb powder 15-25 parts, 20-25 parts of polygonum multiflorum powder, 15-25 parts of chrysanthemum powder, 3-5 parts of licorice powder.
3. a kind of functional thrombolysis enzyme preparation for improving sleep as claimed in claim 2, which is characterized in that contain following weight
The ingredient of part: 15 parts of thrombolysis enzyme powder, 5 parts of bacillus subtilis powder, 30 parts of wild jujube powder, 20 parts of cassia seed powder, 15 parts of Rhizoma Gastrodiae powder, what
25 parts of radix polygoni multiflori powder, 15 parts of chrysanthemum powder, 3 parts of licorice powder.
4. a kind of functional thrombolysis enzyme preparation for improving sleep as described in any one of claims 1-3, which is characterized in that described
Streptokinase powder purity is at least 90%.
5. a kind of functional thrombolysis enzyme preparation for improving sleep as described in any one of claims 1-3, which is characterized in that described
Thrombolysis enzyme preparation is any one in powder, tablet, granule, decoction, oral solution and paste.
6. a kind of preparation method for the functional thrombolysis enzyme preparation for improving sleep a kind of as described in claim any one of 1-5, institute
Stating thrombolysis enzyme preparation is tablet, which comprises the following steps:
Step 1, raw material crushes: wild jujube, cassia seed, Rhizoma Gastrodiae, the fleece-flower root, chrysanthemum, Radix Glycyrrhizae are respectively crushed into powdery, it is spare；
Step 2, it is sieved: the powder raw material in step 1 is crossed into 80-100 mesh screen, it is straight for intransitable progress separating twice
It sieves for subsequent use to whole；
Step 3, thrombolysis enzyme powder is prepared: strain Subtilisin-QK ferments 12 at 25~35 DEG C~for 24 hours, take the upper of fermentation liquid
Clear liquid purifying, freeze-drying, it is spare；
Step 4, it prepares bacillus subtilis powder: the sediment of fermentation liquid in step 3 being taken to wash, be lyophilized, it is spare；
Step 5, mixing, tabletting: wild jujube powder prepared by step 2, step 3, step 4, cassia seed powder, Rhizoma Gastrodiae powder, polygonum multiflorum powder,
Chrysanthemum powder, licorice powder, thrombolysis enzyme powder and bacillus subtilis powder ratio by weight mix, and tabletting is up to the functionality for improving sleep
Streptokinase tablet.
7. a kind of preparation method of functional thrombolysis enzyme preparation for improving sleep as claimed in claim 6, which is characterized in that institute
The preparation that strain Subtilisin-QK in step 3 further includes pancreas egg peptone culture medium before fermentation is stated, by strain Subtilisin-
QK, which is placed in the pancreas egg peptone culture medium, to ferment.
8. a kind of preparation method of functional thrombolysis enzyme preparation for improving sleep as claimed in claim 7, which is characterized in that institute
State pancreas egg peptone culture medium the preparation method comprises the following steps: taking 10~20g of tryptone, 2~8g of phytone, 3~7g of sodium chloride add water
It is spare that 7.0~7.5,121 DEG C of 10~20min of sterilizing are adjusted to 1000ml, PH.
9. a kind of preparation method of functional thrombolysis enzyme preparation for improving sleep as claimed in claim 6, which is characterized in that institute
It states and the supernatant of streptokinase fermentation liquid is at least 4mol/L ammonium sulfate with concentration in step 3 saltouts purifying.
10. a kind of functional streptokinase product application for improving sleep as described in claim any one of 1-6 is in improvement sleep medicine
The preparation of product or health care product.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840525A (en) * | 2020-08-01 | 2020-10-30 | 武汉真福医药股份有限公司 | Composition for treating thromboangiitis and preparation method thereof |
| CN113519837A (en) * | 2021-07-24 | 2021-10-22 | 湖北真福医药有限公司 | Thrombolytic enzyme product capable of improving alcoholism and preparation method and application thereof |
| CN113558238A (en) * | 2021-07-28 | 2021-10-29 | 湖北真福医药有限公司 | Natto freeze-dried powder spina date seed solid beverage and preparation method thereof |
| CN113559130A (en) * | 2021-07-30 | 2021-10-29 | 湖北真福医药有限公司 | Spina date seed and bacillus subtilis co-fermentation product and preparation method and application thereof |
| WO2024062470A1 (en) * | 2022-09-21 | 2024-03-28 | Moraz Medical Herbs (1989) Ltd. | Dermal and cosmetic compositions |
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2019
- 2019-07-04 CN CN201910607778.1A patent/CN110404059A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840525A (en) * | 2020-08-01 | 2020-10-30 | 武汉真福医药股份有限公司 | Composition for treating thromboangiitis and preparation method thereof |
| CN111840525B (en) * | 2020-08-01 | 2023-10-31 | 武汉真福医药股份有限公司 | Composition for treating thromboangiitis and preparation method thereof |
| CN113519837A (en) * | 2021-07-24 | 2021-10-22 | 湖北真福医药有限公司 | Thrombolytic enzyme product capable of improving alcoholism and preparation method and application thereof |
| CN113558238A (en) * | 2021-07-28 | 2021-10-29 | 湖北真福医药有限公司 | Natto freeze-dried powder spina date seed solid beverage and preparation method thereof |
| CN113559130A (en) * | 2021-07-30 | 2021-10-29 | 湖北真福医药有限公司 | Spina date seed and bacillus subtilis co-fermentation product and preparation method and application thereof |
| WO2024062470A1 (en) * | 2022-09-21 | 2024-03-28 | Moraz Medical Herbs (1989) Ltd. | Dermal and cosmetic compositions |
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