CN110382752A - 具有受控化学计量的多核苷酸文库及其合成 - Google Patents

具有受控化学计量的多核苷酸文库及其合成 Download PDF

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Publication number
CN110382752A
CN110382752A CN201780084031.1A CN201780084031A CN110382752A CN 110382752 A CN110382752 A CN 110382752A CN 201780084031 A CN201780084031 A CN 201780084031A CN 110382752 A CN110382752 A CN 110382752A
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polynucleotides
polynucleotide
library
polynucleotide library
sequence
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English (en)
Chinese (zh)
Inventor
拉姆齐·易卜拉欣·泽图恩
陈思远
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Special Biological Science Co Ltd Of Tevez
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Special Biological Science Co Ltd Of Tevez
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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780084031.1A 2016-11-18 2017-11-17 具有受控化学计量的多核苷酸文库及其合成 Pending CN110382752A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662424302P 2016-11-18 2016-11-18
US62/424,302 2016-11-18
US201762548307P 2017-08-21 2017-08-21
US62/548,307 2017-08-21
US201762558666P 2017-09-14 2017-09-14
US62/558,666 2017-09-14
PCT/US2017/062391 WO2018094263A1 (en) 2016-11-18 2017-11-17 Polynucleotide libraries having controlled stoichiometry and synthesis thereof

Publications (1)

Publication Number Publication Date
CN110382752A true CN110382752A (zh) 2019-10-25

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CN201780084031.1A Pending CN110382752A (zh) 2016-11-18 2017-11-17 具有受控化学计量的多核苷酸文库及其合成

Country Status (10)

Country Link
US (2) US20180142289A1 (https=)
EP (1) EP3541973B1 (https=)
JP (3) JP2020504709A (https=)
KR (1) KR102569164B1 (https=)
CN (1) CN110382752A (https=)
AU (2) AU2017363204B2 (https=)
CA (1) CA3044057A1 (https=)
GB (1) GB2572877A (https=)
IL (2) IL266681B2 (https=)
WO (1) WO2018094263A1 (https=)

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CN116417071A (zh) * 2023-04-07 2023-07-11 祥符实验室 一种dna数据存储的选择性访问方法

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WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
JP2020504709A (ja) * 2016-11-18 2020-02-13 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
US11708605B2 (en) 2019-11-06 2023-07-25 Adaptive Biotechnologies Corporation Synthetic strands for nucleic acid sequencing and related methods and systems
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variable nucleic acid libraries for adenosine receptors
MX2022013499A (es) 2020-04-27 2023-01-16 Twist Bioscience Corp Bibliotecas de ácidos nucleicos variantes para coronavirus.
US11655503B2 (en) 2020-05-11 2023-05-23 Personal Genome Diagnostics Inc. Selective capture of target DNA sequences
KR20230034941A (ko) * 2020-05-28 2023-03-10 일루미나, 인코포레이티드 상이한 특징을 갖는 폴리뉴클레오티드 사본 비교
US12391762B2 (en) 2020-08-26 2025-08-19 Twist Bioscience Corporation Methods and compositions relating to GLP1R variants
CA3190917A1 (en) 2020-08-28 2022-03-03 Andres Fernandez Devices and methods for synthesis
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
WO2022159620A1 (en) 2021-01-21 2022-07-28 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
BE1029144B1 (fr) * 2021-02-25 2022-09-20 Oncodna Méthode de caracterisation d'une tumeur à l'aide d'un séquençage ciblé
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
WO2022271884A2 (en) 2021-06-22 2022-12-29 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
EP4388118A4 (en) 2021-08-19 2025-05-21 Twist Bioscience Corporation METHODS AND COMPOSITIONS IN COMPOSITION WITH COVALENTLY CLOSED NUCLEIC ACIDS
IL312714A (en) 2021-11-18 2024-07-01 Twist Bioscience Corp DICKKOPF-1 variant antibodies and methods of use
US12325739B2 (en) 2022-01-03 2025-06-10 Twist Bioscience Corporation Bispecific SARS-CoV-2 antibodies and methods of use

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN110853708A (zh) * 2019-11-13 2020-02-28 上海仁东医学检验所有限公司 用于hla分型的核酸捕获探针的设计方法
CN110853708B (zh) * 2019-11-13 2022-03-08 上海仁东医学检验所有限公司 用于hla分型的核酸捕获探针的设计方法
CN116417071A (zh) * 2023-04-07 2023-07-11 祥符实验室 一种dna数据存储的选择性访问方法
CN116417071B (zh) * 2023-04-07 2026-04-03 祥符实验室 一种dna数据存储的选择性访问方法

Also Published As

Publication number Publication date
KR20190093585A (ko) 2019-08-09
EP3541973A1 (en) 2019-09-25
EP3541973B1 (en) 2024-03-27
IL266681A (en) 2019-07-31
GB2572877A (en) 2019-10-16
AU2017363204B2 (en) 2022-12-08
AU2017363204A1 (en) 2019-07-04
JP2020504709A (ja) 2020-02-13
JP2023017863A (ja) 2023-02-07
IL266681B2 (en) 2025-01-01
IL266681B1 (en) 2024-09-01
EP3541973A4 (en) 2020-07-29
WO2018094263A1 (en) 2018-05-24
US20180142289A1 (en) 2018-05-24
KR102569164B1 (ko) 2023-08-21
IL314890A (en) 2024-10-01
US20210348220A1 (en) 2021-11-11
GB201908719D0 (en) 2019-07-31
AU2023200850A1 (en) 2023-03-16
CA3044057A1 (en) 2018-05-24
JP2025028854A (ja) 2025-03-05

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