KR102569164B1 - 조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성 - Google Patents

조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성 Download PDF

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KR102569164B1
KR102569164B1 KR1020197017281A KR20197017281A KR102569164B1 KR 102569164 B1 KR102569164 B1 KR 102569164B1 KR 1020197017281 A KR1020197017281 A KR 1020197017281A KR 20197017281 A KR20197017281 A KR 20197017281A KR 102569164 B1 KR102569164 B1 KR 102569164B1
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polynucleotide
library
polynucleotides
delete delete
sequence
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KR20190093585A (ko
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램지 이브라힘 자이툰
쓰위엔 천
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트위스트 바이오사이언스 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020197017281A 2016-11-18 2017-11-17 조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성 Active KR102569164B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662424302P 2016-11-18 2016-11-18
US62/424,302 2016-11-18
US201762548307P 2017-08-21 2017-08-21
US62/548,307 2017-08-21
US201762558666P 2017-09-14 2017-09-14
US62/558,666 2017-09-14
PCT/US2017/062391 WO2018094263A1 (en) 2016-11-18 2017-11-17 Polynucleotide libraries having controlled stoichiometry and synthesis thereof

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KR20190093585A KR20190093585A (ko) 2019-08-09
KR102569164B1 true KR102569164B1 (ko) 2023-08-21

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US (2) US20180142289A1 (https=)
EP (1) EP3541973B1 (https=)
JP (3) JP2020504709A (https=)
KR (1) KR102569164B1 (https=)
CN (1) CN110382752A (https=)
AU (2) AU2017363204B2 (https=)
CA (1) CA3044057A1 (https=)
GB (1) GB2572877A (https=)
IL (2) IL266681B2 (https=)
WO (1) WO2018094263A1 (https=)

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CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
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CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
US11708605B2 (en) 2019-11-06 2023-07-25 Adaptive Biotechnologies Corporation Synthetic strands for nucleic acid sequencing and related methods and systems
CN110853708B (zh) * 2019-11-13 2022-03-08 上海仁东医学检验所有限公司 用于hla分型的核酸捕获探针的设计方法
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US11655503B2 (en) 2020-05-11 2023-05-23 Personal Genome Diagnostics Inc. Selective capture of target DNA sequences
KR20230034941A (ko) * 2020-05-28 2023-03-10 일루미나, 인코포레이티드 상이한 특징을 갖는 폴리뉴클레오티드 사본 비교
US12391762B2 (en) 2020-08-26 2025-08-19 Twist Bioscience Corporation Methods and compositions relating to GLP1R variants
CA3190917A1 (en) 2020-08-28 2022-03-03 Andres Fernandez Devices and methods for synthesis
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
WO2022159620A1 (en) 2021-01-21 2022-07-28 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
BE1029144B1 (fr) * 2021-02-25 2022-09-20 Oncodna Méthode de caracterisation d'une tumeur à l'aide d'un séquençage ciblé
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WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
WO2022271884A2 (en) 2021-06-22 2022-12-29 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
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KR20190093585A (ko) 2019-08-09
EP3541973A1 (en) 2019-09-25
EP3541973B1 (en) 2024-03-27
IL266681A (en) 2019-07-31
GB2572877A (en) 2019-10-16
AU2017363204B2 (en) 2022-12-08
AU2017363204A1 (en) 2019-07-04
JP2020504709A (ja) 2020-02-13
JP2023017863A (ja) 2023-02-07
IL266681B2 (en) 2025-01-01
IL266681B1 (en) 2024-09-01
EP3541973A4 (en) 2020-07-29
WO2018094263A1 (en) 2018-05-24
US20180142289A1 (en) 2018-05-24
CN110382752A (zh) 2019-10-25
IL314890A (en) 2024-10-01
US20210348220A1 (en) 2021-11-11
GB201908719D0 (en) 2019-07-31
AU2023200850A1 (en) 2023-03-16
CA3044057A1 (en) 2018-05-24
JP2025028854A (ja) 2025-03-05

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