JP2020504709A - 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成 - Google Patents

制御された化学量論を有するポリヌクレオチドライブラリおよびその合成 Download PDF

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JP2020504709A
JP2020504709A JP2019525959A JP2019525959A JP2020504709A JP 2020504709 A JP2020504709 A JP 2020504709A JP 2019525959 A JP2019525959 A JP 2019525959A JP 2019525959 A JP2019525959 A JP 2019525959A JP 2020504709 A JP2020504709 A JP 2020504709A
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polynucleotides
polynucleotide
library
percent
sequence
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JP2020504709A5 (https=
Inventor
イブライム ゼイトン，ラムゼイ
イブライム ゼイトン，ラムゼイ
チェン，スーユアン
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ツイスト バイオサイエンス コーポレーション
ツイスト バイオサイエンス コーポレーション
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Publication of JP2020504709A publication Critical patent/JP2020504709A/ja
Publication of JP2020504709A5 publication Critical patent/JP2020504709A5/ja
Priority to JP2022173718A priority Critical patent/JP2023017863A/ja
Priority to JP2024192262A priority patent/JP2025028854A/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019525959A 2016-11-18 2017-11-17 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成 Pending JP2020504709A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022173718A JP2023017863A (ja) 2016-11-18 2022-10-28 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2024192262A JP2025028854A (ja) 2016-11-18 2024-10-31 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662424302P 2016-11-18 2016-11-18
US62/424,302 2016-11-18
US201762548307P 2017-08-21 2017-08-21
US62/548,307 2017-08-21
US201762558666P 2017-09-14 2017-09-14
US62/558,666 2017-09-14
PCT/US2017/062391 WO2018094263A1 (en) 2016-11-18 2017-11-17 Polynucleotide libraries having controlled stoichiometry and synthesis thereof

Related Child Applications (1)

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JP2022173718A Division JP2023017863A (ja) 2016-11-18 2022-10-28 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成

Publications (2)

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JP2020504709A true JP2020504709A (ja) 2020-02-13
JP2020504709A5 JP2020504709A5 (https=) 2020-12-24

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JP2019525959A Pending JP2020504709A (ja) 2016-11-18 2017-11-17 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2022173718A Pending JP2023017863A (ja) 2016-11-18 2022-10-28 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2024192262A Pending JP2025028854A (ja) 2016-11-18 2024-10-31 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成

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JP2022173718A Pending JP2023017863A (ja) 2016-11-18 2022-10-28 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2024192262A Pending JP2025028854A (ja) 2016-11-18 2024-10-31 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成

Country Status (10)

Country Link
US (2) US20180142289A1 (https=)
EP (1) EP3541973B1 (https=)
JP (3) JP2020504709A (https=)
KR (1) KR102569164B1 (https=)
CN (1) CN110382752A (https=)
AU (2) AU2017363204B2 (https=)
CA (1) CA3044057A1 (https=)
GB (1) GB2572877A (https=)
IL (2) IL266681B2 (https=)
WO (1) WO2018094263A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
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JP2023017863A (ja) * 2016-11-18 2023-02-07 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2023528003A (ja) * 2020-05-28 2023-07-03 イルミナ インコーポレイテッド 異なる特徴を有するポリヌクレオチドのコピーの比較

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KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
JP7129343B2 (ja) * 2016-05-02 2022-09-01 ベクトン・ディキンソン・アンド・カンパニー 正確な分子バーコーディング
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
EP4055159A4 (en) 2019-11-06 2024-04-17 Adaptive Biotechnologies Corporation SYNTHETIC STRANDS FOR NUCLEIC ACID SEQUENCING AND RELATED METHODS AND SYSTEMS
CN110853708B (zh) * 2019-11-13 2022-03-08 上海仁东医学检验所有限公司 用于hla分型的核酸捕获探针的设计方法
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
MX2022013499A (es) 2020-04-27 2023-01-16 Twist Bioscience Corp Bibliotecas de ácidos nucleicos variantes para coronavirus.
KR20230034950A (ko) * 2020-05-11 2023-03-10 퍼스널 게놈 다이아그노스틱스 인코포레이티드 표적 dna 서열의 선택적 포획
EP4204460A4 (en) 2020-08-26 2024-09-25 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS
CN117043171A (zh) 2020-08-28 2023-11-10 特韦斯特生物科学公司 用于合成的装置和方法
EP4229210A4 (en) 2020-10-19 2025-01-08 Twist Bioscience Corporation METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
BE1029144B1 (fr) * 2021-02-25 2022-09-20 Oncodna Méthode de caracterisation d'une tumeur à l'aide d'un séquençage ciblé
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CN116417071B (zh) * 2023-04-07 2026-04-03 祥符实验室 一种dna数据存储的选择性访问方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023017863A (ja) * 2016-11-18 2023-02-07 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
JP2023528003A (ja) * 2020-05-28 2023-07-03 イルミナ インコーポレイテッド 異なる特徴を有するポリヌクレオチドのコピーの比較

Also Published As

Publication number Publication date
EP3541973B1 (en) 2024-03-27
KR20190093585A (ko) 2019-08-09
KR102569164B1 (ko) 2023-08-21
GB201908719D0 (en) 2019-07-31
JP2025028854A (ja) 2025-03-05
EP3541973A1 (en) 2019-09-25
JP2023017863A (ja) 2023-02-07
EP3541973A4 (en) 2020-07-29
US20180142289A1 (en) 2018-05-24
AU2017363204A1 (en) 2019-07-04
IL314890A (en) 2024-10-01
AU2017363204B2 (en) 2022-12-08
US20210348220A1 (en) 2021-11-11
IL266681B1 (en) 2024-09-01
AU2023200850A1 (en) 2023-03-16
IL266681B2 (en) 2025-01-01
CA3044057A1 (en) 2018-05-24
IL266681A (en) 2019-07-31
WO2018094263A1 (en) 2018-05-24
GB2572877A (en) 2019-10-16
CN110382752A (zh) 2019-10-25

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