CN110367066A - A kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters - Google Patents
A kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters Download PDFInfo
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Abstract
The present invention discloses a kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters, belong to agricultural production and studying technological domain, this method is established using the comprehensive drought resistance coefficient of the i.e. more gradient multiple characters of new synthesis drought resistance coefficient as the method for the evaluation paddy drought resistance of evaluation criterion, it proposes under the conditions of more gradients quantify control water, obtain the drought resistance coefficient of the multiple characters of rice, and the foundation that paddy drought resistance size compares is made by the comprehensive drought resistance coefficient of more gradient multiple characters of rice, it solves existing evaluation method and lacks the fuzzy qualitative evaluation defect quantified under the conditions of control water, and result is not comprehensive enough, it is difficult to repeat, the shortcomings that universal standard method can not be formed, more accurately paddy drought resistance can be evaluated, scientific evaluation foundation is provided for Drought Resistance in Rice Varieties evaluation and Drought-resistant Breeding.
Description
Technical field
The invention belongs to field of agricultural production technologies, more particularly to one kind based on the comprehensive drought resistance coefficient of more gradient multiple characters
Paddy drought resistance evaluation method.
Background technique
Rice is one of most important cereal crops of the mankind and the maximum crop of water consumption.Make production mould in Traditional Rice
Under formula, water production efficiency (WaterUseEffcency water use efficiency) is 0.6~1.04kg paddy/m3Water, i.e., it is raw
Producing 1kg paddy about needs 0.96~1.67m3Water.Since the reduction of agriculture arable area and population expansion, rice demand constantly increase
Add, while regional and seasonal drought takes place frequently and the problems such as agricultural water resources are gradually deficient, make rice make production be faced with it is tight
It challenges again.Therefore Study On Rice saving water, resisting drought technology, development saving water, resisting drought rice become the important way that rice makees production sustainable development
Diameter.The paddy drought resistance identification of indicator reported at present is broadly divided into the types such as yield index, morphological index and physiological and biochemical index,
Propose the concepts such as drought resistance coefficient, Sensitivity Index, antiG valves and direct comparison method, drought resistance grading method, total drought resisting
The property integrated evaluating methods such as evaluation assessment and mathematical methods.Since drought resistance is multifactor exercising result, different cultivars and difference
The drought resistance of developmental stage is not quite similar, so that certain complexity is presented in paddy drought resistance, a variety of identification methods and refers in addition
The report of mark and result, not yet forms unified generally acknowledged Identification of Drought standard, and people are still difficult to effectively hold the anti-of rice
Drought identification.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of Rice Drought Resistence based on the comprehensive drought resistance coefficient of more gradient multiple characters
Property integrated evaluating method, this method establishes with the comprehensive drought resistance coefficients of new synthesis drought resistance coefficient i.e. more gradient multiple characters as evaluation
The method of the evaluation paddy drought resistance of standard solves existing evaluation method and lacks the fuzzy qualitative evaluation quantified under the conditions of control water
Defect and result are not comprehensive enough, are difficult to repeat, can not form the shortcomings that universal standard method, can be more accurately to Rice Resistance
Drought is evaluated, and provides scientific evaluation foundation for Drought Resistance in Rice Varieties evaluation and Drought-resistant Breeding.
For this purpose, the technical solution adopted by the present invention is that:
A kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters, this method includes following step
It is rapid:
(1) a variety of rice varieties to be evaluated are determined, seedling respectively obtains each kind rice seedlings；
(2) potted plant experiment is carried out to each kind rice seedlings respectively, the potted plant experiment includes experimental group and control group, the reality
It tests group and control group refers to that the rice seedlings are carried out with gradient quantization control hydroponics and waterflooding respectively cultivates, and remaining experiment condition is equal
It is identical, respectively obtain the mature rice of experimental group and control group；
Steps are as follows for the gradient quantization control hydroponics:
A, prepare multiple identical cultivation basins, be respectively charged into the cultivating soil of identical weight, and the field for measuring the soil is held
Water and initial water content；
B, the kind rice seedlings are transplanted into basin, obtain rice potting, single basin rice seedlings strain number is identical, according to saturation moisture,
Mild drought stress, medium drought and Severe drought coerce 4 kinds of gradients, and set 4 kinds of gradient series according to 4 kinds of gradients,
Then it calculates separately to obtain 4 kinds of gradient moisture contents of the rice potting；
C, according to 4 kinds of gradient moisture contents, rice potting is divided into 4 groups, and ladder is carried out to each group respectively
Metrization water control pipe reason records the rate of water make-up of each group single basin every time, obtains each group's list basin and add up rate of water make-up, until rice at
It is ripe；
(3) species test is sampled to the mature rice of the experimental group and control group respectively, measures two groups of rice respectively
Each character species test data；Each character species test data include single plant biological yield, a single plant's output, the effective fringe of single plant
Number, single fringe bear fruit grains and mass of 1000 kernel, each character species test data refer to the average data of each character species test；
(4) according to each character species test data of two groups of rice in step (3), the experimental group and control group water are respectively obtained
The structure of output character of rice, and the water use efficiency of two groups of rice is calculated, the structure of output character includes single plant seed
Grain yield, panicle number per plant, single fringe bear fruit grains and mass of 1000 kernel, the water use efficiency include single plant biological yield moisture
Utilization efficiency and a single plant's output water use efficiency；
(5) according to the structure of output character and water use efficiency of experimental group and control group rice obtained by step (4), respectively
Calculate the structure of output character of the experimental group and control group rice and each character drought resistance coefficient of water use efficiency；
(6) the resulting each drought resistance coefficient of step (5) is made into xy axial coordinate figure with 4 kinds of gradient moisture contents respectively, point
Each more gradient unisexuality shape coordinate diagrams of the experimental group and control group rice are not obtained, and each more gradients are obtained by calculation
The area for each figure that unisexuality shape coordinate diagram and two reference axis of xy are surrounded, the area of each figure be the experimental group and
Each character T value of control group rice；
(7) according to each character T value of experimental group and control group rice obtained by step (6), the rice varieties are calculated
The comprehensive drought resistance coefficient of more gradient multiple characters, by comparing the comprehensive drought resistance coefficient size of more gradient multiple characters of each rice varieties,
With the height of each Drought Resistance in Rice Varieties of determination.
Measurement potting soil initial water content and field water holding in above-mentioned steps (2) as a further optimization of the solution,
The step of amount specifically:
A, undisturbed soil is taken in cultivation basin with cutting ring, the undisturbed soil weight is weighed using cutting ring weight method and is recorded as
W0；
B, the undisturbed soil is dried and is weighed, and be recorded as W1, then by its levigate 1mm sieve pore excessively, it is fitted into cutting ring, it will
There is the cutting ring bottom cover of aperture and attached filter paper to cover, be put into the disk being filled with water, water surface 1-2mm low compared with cutting ring upper limb, makes ring in disk
Soil sufficiently absorbs water in knife, and saturation is reached after diel, goes bottom cover to weigh and is recorded as W2；
The field capacity calculation formula is as follows:
FM=[(W2- W0)/W1] × 100%
In formula, FM indicates field capacity；
The initial water content calculation formula is as follows:
Wc=[(W0- W1)/W0] × 100%
In formula, WcIndicate initial water content.
Gradient series is ﹥ 100%, 80%, 60% and 40% in above-mentioned steps (2) as a further optimization of the solution,.
Above-mentioned rice seedlings are by every 5~10 basin of gradient, 3 cave of single basin, every cave 1-2 plants of progress as a further optimization of the solution,
Net transplantation of seedlings.
The calculation formula of above-mentioned gradient moisture content is as follows as a further optimization of the solution:
Wt=FM × gradient series
In formula, WtIndicate gradient moisture content, FM indicates field capacity.
Gradient quantization water control pipe manages specific steps in above-mentioned steps (2) as a further optimization of the solution, are as follows:
A, according to gradient moisture content, the basin soil weight of 4 group is calculated separately, calculation formula is as follows:
Gp=mono- basin dry ground weight × (1+Wt)+plant basin net weight
In formula, GpIndicate basin soil weight, WtIndicate gradient moisture content, the list basin dry ground weight is by all soil in single basin
Weight after earth drying；
B, every group's basin soil weight is calculated as G respectivelypThen i, i=1.2.3.4 are poured into each group's potting enough respectively
Water makes rice cultivate state in waterflooding, is placed in the shady place of illumination abundance, is reduced naturally to moisture in the potting to basin soil
Weight is GpWhen i, periodically weigh to the basin soil by basin by group, and moisturizing makes the basin soil keep G on demandpI constant weight, and point
Each moisturizing weight is not recorded by group, the single basin for obtaining each group adds up rate of water make-up；The basin soil weight be plant basin and
The gross weight of aqueous soil in basin.
Specific step is as follows for above-mentioned steps (3) as a further optimization of the solution:
A, it after rice is mature, gathers in and species test is sampled to the experimental group and control group respectively, respectively obtain experiment
The single plant biological yield of group and control group, a single plant's output, panicle number per plant, single fringe bear fruit grains and each species test of mass of 1000 kernel
Data；
B, the biological yield water use efficiency and grain yield moisture benefit of the experimental group and control group is calculated
With efficiency, calculation formula is as follows:
The mono- mono- basin total water consumption of basin total strain number × B/ of BW=
The mono- mono- basin total water consumption of basin total strain number × G/ of GW=
In formula, it is raw that BW, B, GW and G respectively indicate the biological yield water use efficiency of experimental group and control group, single plant
Object yield, grain yield water use efficiency and a single plant's output, single basin total water consumption=mono- basin soil antecedent water content+
Single basin adds up single basin soil water content after rate of water make-up-harvest, wherein the measurement method of single basin soil water content after harvest
It is identical as the measurement method of single basin soil antecedent water content.
Above-mentioned steps (4) each character drought resistance coefficient is indicated as a further optimization of the solution, with DC, calculation formula
It is as follows:
DCG=GIt is real/Gck× 100%
DCP=PIt is real/Pck× 100%
DCF=FIt is real/Fck× 100%
DCK=KIt is real/Kck× 100%
DCBW=BWIt is real/BWck× 100%
DCGW=GWIt is real/GWck× 100%
In formula, DCG、DCP、DCF、DCK、DCBWAnd DCGWRespectively indicate a single plant's output of the rice varieties, single plant has
Imitate spike number, single fringe bear fruit grains, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency drought resisting system
Number；GIt is real、PIt is real、FIt is real、KIt is real、BWIt is realAnd GWIt is realRespectively indicate a single plant's output, panicle number per plant, the Dan Sui of 4 groups in experimental group
Bear fruit grains, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency average；Gck、Pck、Fck、
Kck、BWckAnd GWckRespectively indicate a single plant's output, panicle number per plant, single fringe bear fruit grains, mass of 1000 kernel, biology of control group
Learn the average of yield water use efficiency and grain yield water use efficiency.
More gradient multiple characters integrate drought resistance coefficients by T in above-mentioned steps (6) as a further optimization of the solution,SDCTable
Show, calculation formula is as follows:
TSDC=logT_G(TP×TF×TK)×logT_GWTBW
In formula, T_G, TP、TF、TK, T_GW and TBWRespectively indicate a single plant's output, panicle number per plant, single fringe reality grain
Number, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency T value.
The characteristics of paddy drought resistance integrated evaluating method of the invention, is:
(1) gradient Quantitative design and control are carried out to paddy soil moisture content；
(2) it takes into account Rice Yield Structure character and water use efficiency carries out the building of paddy drought resistance comprehensive evaluation index；
(3) quantify control water using the comprehensive drought resistance coefficient of more gradient multiple characters based on single gradient unisexuality shape drought resistance coefficient as gradient
Rice under Condition Comprehensive Evaluation of Drought Resistance index.
The theoretical foundation of paddy drought resistance integrated evaluating method of the invention is:
The present invention quantifies control water test, yield and its correlated traits by gradient and measures and statistically analyze, and seeks Rice Resistance
Non-irrigated breeding medium drought resistant identifies the compound assessment indicator system that adoptable criterion and quantity controllably repeats, and comments for paddy drought resistance
Valence, saving water, resisting drought rice genetic breeding etc. provide assessment technique index and support.
The drought resistance of rice depends on the interaction of nature-nurture, in the case where consistent and repeatable in environmental condition, product
The drought resistance of inter-species can be just able to correctly relatively.For drought resistance, then mainly soil water content.But soil water content
With dynamic change and uncertainty, often results in drought resistance test result and be difficult to repeat, and test of many times result is also possible to not
Unanimously.This makes paddy drought resistance identification encounter great difficulty.The present invention uses 4 kinds of quantization drought stress, forms 4 kinds of tools
Have the gradation of moisture of extremely significant difference, and structure of output to tested variety and each character of water use efficiency produce it is extremely significant
It influences.The test process has clear and fine control to soil water content, and result is stable and repeatable.
The present invention is by a single plant's output, panicle number per plant, single fringe bear fruit grains, mass of 1000 kernel, single plant biological yield water
Point utilization efficiency and a single plant's output water use efficiency under various drought stress conditions Water Ecology gross effect, that is, T value it
The overall target that product is evaluated as Drought Resistance of Variety, is found to have good identification effect.
Using the technical program the utility model has the advantages that
1, the present invention makes to join by quantifying drought stress to the long-term gradient that rice varieties of participating in the experiment are turned green to harvest
The drought resisting sex differernce of examination Rice Yield is sufficiently showed, and using yield and water use efficiency as drought resistance
Primary evaluation foundation, it is contemplated that breeding character selects and the water-saving requirement of agricultural production, examines after harvest Correlated Yield Characters
Kind, in conjunction with moisture solution process record data, the comprehensive drought resistance coefficient of rice drought tolerance is calculated as rice varieties drought tolerance
Comprehensive evaluation index can more accurately evaluate paddy drought resistance, provide foundation for Rice Germplasm Resources screening of drought resistance.
2, the present invention exists as a kind of completely new integrated evaluating method, the present invention for rice yield and water use efficiency
Main affecting factors-soil water content in drought stress environment, as the master control among the appraisement system of paddy drought resistance
Factor has been put forward for the first time the concept and its specific method of gradient quantization drought stress, and soil water content is in actual treatment mistake
Cheng Zhongcheng frequency ventilating type regular fluctuation, each gradient peak are stablized in consistent level, and the more gradient multiple characters moisture of rice are proposed
New direction has been verified in ecological gross effect T value concept and its calculation method, the research for paddy drought resistance from now on.
3, the investigation mode of more gradient multiple characters combines the double factor of rice itself heredity and growing environment, gained
As a result more acurrate, reasonable, and will not change because of changes in environmental conditions such as year, soil types, it is versatile.
Detailed description of the invention
The present invention is described in further detail below in conjunction with the accompanying drawings.
The gradient of Fig. 1 soil water content of the present invention quantifies control model figure.
In Fig. 2 the embodiment of the present invention, 6 test variety character T values calculate schematic diagram.
Drought resistance coefficient figure of the 6 test variety a single plant's outputs of Fig. 3 in different gradient soil water contents.
Drought resistance coefficient figure of the 6 test variety panicle number per plants of Fig. 4 in different gradient soil water contents.
Drought resistance coefficient figure of the 6 test variety list fringe bear fruit grains of Fig. 5 in different gradient soil water contents.
Drought resistance coefficient figure of the 6 test variety mass of 1000 kernel of Fig. 6 in different gradient soil water contents.
Drought resistance coefficient of the 6 test variety biological yield water use efficiency of Fig. 7 in different gradient soil water contents
Figure.
Drought resistance coefficient of the 6 test variety grain yield water use efficiency of Fig. 8 in different gradient soil water contents
Figure.
The T value of the character of test variety 6 of Fig. 96 compares figure.
The comprehensive drought resistance coefficient T of the more gradient multiple characters of 6 test varieties of Figure 10SDCValue compares figure.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, right in the following with reference to the drawings and specific embodiments
The present invention is further elaborated.
Embodiment one:
A kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters, this method includes following step
It is rapid:
(1) prepare following rice varieties: bright extensive 63 (MH63), another name for Sichuan Province extensive 527 (SH527), II-32B, Bala, R17739-1
And IR64, respectively seedling obtain each kind rice seedlings；
(2) potted plant experiment is carried out to each kind rice seedlings respectively, the potted plant experiment includes experimental group and control group, the reality
It tests group and control group refers to that the rice seedlings are carried out with gradient quantization control hydroponics and waterflooding respectively cultivates, and remaining experiment condition is equal
It is identical, respectively obtain the mature rice of experimental group and control group；
Steps are as follows for the gradient quantization control hydroponics:
A, prepare multiple identical cultivation basins, be respectively charged into the cultivating soil of identical weight, and the field for measuring the soil is held
Water and initial water content；
B, the kind rice seedlings are transplanted into basin, by every 6 basin of gradient, 3 cave of single basin, 1 plant of every cave carries out net transplantation of seedlings, obtains
Rice potting, single basin rice seedlings strain number is identical, coerces according to saturation moisture, mild drought stress, medium drought and Severe drought
Compel 4 kinds of gradients, and set 4 kinds of gradient series according to 4 kinds of gradients, is i.e. then ﹥ 100%, 80%, 60% and 40% is calculated separately
Obtain 4 kinds of gradient moisture contents of the rice potting；
C, according to 4 kinds of gradient moisture contents, rice potting is divided into 4 groups, and ladder is carried out to each group respectively
Metrization water control pipe reason records the rate of water make-up of each group single basin every time, obtains each group's list basin and add up rate of water make-up, until rice at
It is ripe；
(3) species test is sampled to the mature rice of the experimental group and control group respectively, measures two groups of rice respectively
Each character species test data；Each character species test data include single plant biological yield, a single plant's output, the effective fringe of single plant
Number, single fringe bear fruit grains and mass of 1000 kernel, each character species test data refer to the average data of each character species test；
(4) according to each character species test data of two groups of rice in step (3), the experimental group and control group water are respectively obtained
The structure of output character of rice, and the water use efficiency of two groups of rice is calculated, the structure of output character includes single plant seed
Grain yield, panicle number per plant, single fringe bear fruit grains and mass of 1000 kernel, the water use efficiency include single plant biological yield moisture
Utilization efficiency and a single plant's output water use efficiency；
(5) according to the structure of output character and water use efficiency of experimental group and control group rice obtained by step (4), respectively
Calculate the structure of output character of the experimental group and control group rice and each character drought resistance coefficient of water use efficiency；
(6) the resulting each drought resistance coefficient of step (5) is made into xy axial coordinate figure with 4 kinds of gradient moisture contents respectively, point
Each more gradient unisexuality shape coordinate diagrams of the experimental group and control group rice are not obtained, and each more gradients are obtained by calculation
The area for each figure that unisexuality shape coordinate diagram and two reference axis of xy are surrounded, the area of each figure be the experimental group and
Each character T value of control group rice；
(7) according to each character T value of experimental group and control group rice obtained by step (6), the rice varieties are calculated
The comprehensive drought resistance coefficient of more gradient multiple characters, by comparing the comprehensive drought resistance coefficient size of more gradient multiple characters of each rice varieties,
With the height of each Drought Resistance in Rice Varieties of determination.
The step of potting soil initial water content and field capacity are measured in above-mentioned steps (2) specifically:
A, undisturbed soil is taken in cultivation basin with cutting ring, the undisturbed soil weight is weighed using cutting ring weight method and is recorded as
0.285kg；
B, the undisturbed soil is dried and is weighed, and be recorded as 0.241kg, then by its levigate 1mm sieve pore excessively, be packed into cutting ring
In, there will be the cutting ring bottom cover of aperture and attached filter paper to cover, is put into the disk being filled with water, water surface 1-2mm low compared with cutting ring upper limb in disk,
So that soil in cutting ring is sufficiently absorbed water, saturation is reached after diel, goes bottom cover to weigh and be recorded as 0.341kg；
Calculate water-retaining quantity among field of soil
FM=[(0.341-0.241)/0.241] × 100%=41.3%
Calculate soil antecedent water content Wc=15.345%
Calculate above-mentioned 4 kinds of gradient moisture contents:
Wt1﹥ 100%, Wt2=33.04%, Wt2=24.78%, Wt4=16.52%；
Gradient quantization water control pipe manages specific steps in above-mentioned steps (2) are as follows:
A, according to gradient moisture content, the basin soil weight G of 4 group is calculated separatelyp1=9.50kg, Gp2=7.82kg,
Gp3=7.14kg, Gp4=6.40kg；
B, enough water is poured into each group's potting respectively, so that rice is cultivated state in waterflooding, is placed in the yin of illumination abundance
Liang Chu, being reduced naturally to moisture in the potting to basin soil weight is GpWhen i, periodically weigh to the basin soil by basin by group,
And moisturizing makes the basin soil keep G on demandpI constant weight, i=1.2.3.4, and each moisturizing weight is recorded by group respectively, it obtains
Single basin total water consumption of each group, experimental group list basin total water consumption is Wt, control group is Wck；The basin soil weight is
Plant the gross weight of aqueous soil in basin and basin.
Specific step is as follows for above-mentioned steps (3):
A, it after rice is mature, gathers in and species test is sampled to the experimental group and control group respectively, respectively obtain experiment
The single plant biological yield of group and control group, a single plant's output, panicle number per plant, single fringe bear fruit grains and each species test of mass of 1000 kernel
Data see the table below 1；
1 yield of table and structural character and water use efficiency measured value
B, the biological yield water use efficiency and grain yield moisture benefit of the experimental group and control group is calculated
With efficiency, calculation formula is as follows:
BWIt is real=3 × BIt is real/ experimental group list basin total water consumption=3 × BIt is real/Wt
GWIt is real=3 × GIt is real/ experimental group list basin total water consumption=3 × GIt is real/Wt
BWIt is right=3 × Bck/ control group list basin total water consumption=3 × Bck/Wck
GWIt is right=3 × Gck/ control group list basin total water consumption=3 × Gck/Wck
Calculating above-mentioned steps (4) described each character drought resistance coefficient indicates that calculation formula is as follows with DC, calculated result such as table 2
It is shown:
DCG=GIt is real/Gck× 100%
DCP=PIt is real/Pck× 100%
DCF=FIt is real/Fck× 100%
DCK=KIt is real/Kck× 100%
DCBW=BWIt is real/BWck× 100%
DCGW=GWIt is real/GWck× 100%
Each character drought resistance coefficient of each rice varieties of 2 embodiment of table 1
| Bright extensive 63 | Another name for Sichuan Province extensive 527 | II-32B | Bala | R17739-1 | IR64 | |
| DCG | 0.634 | 0.575 | 0.648 | 0.583 | 0.729 | 0.748 |
| DCP | 0.468 | 0.531 | 0.542 | 0.61 | 0.504 | 0.493 |
| DCF | 0.662 | 0.673 | 0.692 | 0.683 | 0.662 | 0.71 |
| DCK | 0.432 | 0.406 | 0.466 | 0.453 | 0.526 | 0.493 |
| DCBW | 0.498 | 0.42 | 0.513 | 0.499 | 0.554 | 0.625 |
| DCGW | 0.838 | 0.683 | 0.879 | 0.834 | 0.809 | 0.809 |
The comprehensive drought resistance coefficient of more gradient multiple characters is by T in above-mentioned steps (6)SDCIt indicates, calculation formula is as follows:
TSDC=logT_G(TP×TF×TK)×logT_GWTBW
In formula, T_G, TP、TF、TK, T_GW and TBWRespectively indicate a single plant's output, panicle number per plant, single fringe reality grain
Number, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency T value, calculated result such as 3 institute of table
Show:
More gradient multiple characters drought resistance coefficient T of each rice varieties of 3 embodiment of table 1SDC
| Bright extensive 63 | Another name for Sichuan Province extensive 527 | Ⅱ-32B | Bala | R17739-1 | IR64 | |
| TSDC | 2.6 | 3.7 | 2.6 | 3.0 | 3.0 | 1.3 |
It can be seen from Table 3 that in terms of drought resistance, extensive (bright extensive 63, II-32B) 527 > (R17739-1, Bala) > in another name for Sichuan Province
> IR64, tallies with the actual situation.
Wherein IR64 be internationally recognized moisture-sensitive kind (Wen-Sheng Wang et al., 2011；Jan-Xang
Lu et al., 2011；PrceAH et al., 2002a；PrceAH et al, 2002b), restorer can be done；Bala is Sichuan
The germplasm resourses with drought resistance that province academy of agricultural sciences crop is filtered out also has document report (Prce AH et al., 2002a；Prce AH
Et al., 2002b) it supports；Bright extensive 63, the backbone parent that another name for Sichuan Province is extensive 527, II-32B is hybrid rice, it was reported that II-32B also has
Certain drought resistance；R17739-1 is the saving water, resisting drought rice restorer of crop institute, academy of agricultural sciences, Sichuan Province breeding.Therefore, rice varieties
More gradient multiple characters drought resistance coefficient TSDC it is bigger, drought resistance is better.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (9)
1. a kind of paddy drought resistance evaluation method based on the comprehensive drought resistance coefficient of more gradient multiple characters, which is characterized in that this method
The following steps are included:
(1) a variety of rice varieties to be evaluated are determined, seedling respectively obtains each kind rice seedlings；
(2) potted plant experiment is carried out to each kind rice seedlings respectively, the potted plant experiment includes experimental group and control group, the experimental group
Refer to control group and the rice seedlings are carried out with gradient quantization control hydroponics and waterflooding cultivation respectively, and remaining experiment condition is homogeneous
Together, the mature rice of experimental group and control group is respectively obtained；
Steps are as follows for the gradient quantization control hydroponics:
A, prepare multiple identical cultivation basins, be respectively charged into the cultivating soil of identical weight, and measure the field capacity of the soil
And initial water content；
B, the kind rice seedlings are transplanted into basin, obtain rice potting, single basin rice seedlings strain number is identical, according to saturation moisture, slightly
Drought stress, medium drought and Severe drought coerce 4 kinds of gradients, and set 4 kinds of gradient series according to 4 kinds of gradients, then
It calculates separately to obtain 4 kinds of gradient moisture contents of the rice potting；
C, according to 4 kinds of gradient moisture contents, rice potting is divided into 4 groups, and gradient amount is carried out to each group respectively
Change water control pipe reason, records the rate of water make-up of each group single basin every time, obtain each group's list basin and add up rate of water make-up, until rice is mature；
(3) species test is sampled to the mature rice of the experimental group and control group respectively, measures each property of two groups of rice respectively
Shape species test data；Each character species test data include single plant biological yield, a single plant's output, panicle number per plant, list
Fringe bear fruit grains and mass of 1000 kernel, each character species test data refer to the average data of each character species test；
(4) according to each character species test data of two groups of rice in step (3), the experimental group and control group rice are respectively obtained
Structure of output character, and the water use efficiency of two groups of rice is calculated, the structure of output character includes that single plant seed produces
Amount, panicle number per plant, single fringe bear fruit grains and mass of 1000 kernel, the water use efficiency include single plant biological yield water utilization
Efficiency and a single plant's output water use efficiency；
(5) it according to the structure of output character and water use efficiency of experimental group and control group rice obtained by step (4), calculates separately
Each character drought resistance coefficient of the structure of output character and water use efficiency of the experimental group and control group rice；
(6) the resulting each drought resistance coefficient of step (5) is made into xy axial coordinate figure with 4 kinds of gradient moisture contents respectively, respectively
To each more gradient unisexuality shape coordinate diagrams of the experimental group and control group rice, and each more gradient unisexuality are obtained by calculation
The area for each figure that shape coordinate diagram and two reference axis of xy are surrounded, the area of each figure is the experimental group and control
Each character T value of group rice；
(7) according to each character T value of experimental group and control group rice obtained by step (6), the more of the rice varieties are calculated
Gradient multiple characters integrate drought resistance coefficient, by comparing the comprehensive drought resistance coefficient size of more gradient multiple characters of each rice varieties, with true
The height of fixed each Drought Resistance in Rice Varieties.
2. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that the step of potting soil initial water content and field capacity are measured in the step (2) specifically:
A, undisturbed soil is taken in cultivation basin with cutting ring, the undisturbed soil weight is weighed using cutting ring weight method and is recorded as W0；
B, the undisturbed soil is dried and is weighed, and be recorded as W1, then by its levigate 1mm sieve pore excessively, it is fitted into cutting ring, will has small
The cutting ring bottom cover of hole and attached filter paper covers, and is put into the disk being filled with water, and water surface 1-2mm low compared with cutting ring upper limb, makes in cutting ring in disk
Soil sufficiently absorbs water, and saturation is reached after diel, goes bottom cover to weigh and is recorded as W2；
The field capacity calculation formula is as follows:
FM=[(W2- W0)/W1] × 100%
In formula, FM indicates field capacity；
The initial water content calculation formula is as follows:
Wc=[(W0- W1)/W0] × 100%
In formula, WcIndicate initial water content.
3. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that gradient series is ﹥ 100%, 80%, 60% and 40% in the step (2).
4. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that for the rice seedlings by every 5~10 basin of gradient, 3 cave of single basin, every cave 1-2 plants carry out net transplantation of seedlings.
5. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that the calculation formula of the gradient moisture content is as follows:
Wt=FM × gradient series
In formula, WtIndicate gradient moisture content, FM indicates field capacity.
6. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that gradient quantization water control pipe manages specific steps in the step (2) are as follows:
A, according to gradient moisture content, the basin soil weight of 4 group is calculated separately, calculation formula is as follows:
Gp=mono- basin dry ground weight × (1+Wt)+plant basin net weight
In formula, GpIndicate basin soil weight, WtIndicate gradient moisture content, the list basin dry ground weight is to dry soil all in single basin
Weight after dry；
B, every group's basin soil weight is calculated as G respectivelypThen i, i=1.2.3.4 pour into enough water to each group's potting respectively, make water
Rice cultivates state in waterflooding, is placed in the shady place of illumination abundance, is reduced naturally to moisture in the potting to basin soil weight and is
GpWhen i, periodically weigh to the basin soil by basin by group, and moisturizing makes the basin soil keep G on demandpI constant weight, and respectively by small
Group records each moisturizing weight, and the single basin for obtaining each group adds up rate of water make-up；The basin soil weight is that cultivation basin and basin include
The gross weight of water and soil earth.
7. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that specific step is as follows for the step (3):
A, after rice is mature, gathers in and species test simultaneously be sampled to the experimental group and control group respectively, respectively obtain experimental group and
Single plant biological yield, a single plant's output, panicle number per plant, single fringe bear fruit grains and each species test number of mass of 1000 kernel of control group
According to；
B, it calculates separately to obtain the biological yield water use efficiency and grain yield moisture benefit of the experimental group and control group
With efficiency, calculation formula is as follows:
The mono- mono- basin total water consumption of basin total strain number × B/ of BW=
The mono- mono- basin total water consumption of basin total strain number × G/ of GW=
In formula, BW, B, GW and G respectively indicate the biological yield water use efficiency of experimental group and control group, single plant biology
Yield, grain yield water use efficiency and a single plant's output, single basin total water consumption=mono- basin soil antecedent water content+mono- basin
Single basin soil water content after accumulative rate of water make-up-harvest, wherein the measurement method and list of single basin soil water content after harvest
The measurement method of basin soil antecedent water content is identical.
8. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that step (4) each character drought resistance coefficient indicates that calculation formula is as follows with DC:
DCG=GIt is real/Gck× 100%
DCP=PIt is real/Pck× 100%
DCF=FIt is real/Fck× 100%
DCK=KIt is real/Kck× 100%
DCBW=BWIt is real/BWck× 100%
DCGW=GWIt is real/GWck× 100%
In formula, DCG、DCP、DCF、DCK、DCBWAnd DCGWRespectively indicate the effective fringe of a single plant's output, single plant of the rice varieties
Number, single fringe bear fruit grains, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency drought resistance coefficient；
GIt is real、PIt is real、FIt is real、KIt is real、BWIt is realAnd GWIt is realRespectively indicate a single plant's output, panicle number per plant of 4 groups in experimental group, single fringe reality grain
Number, mass of 1000 kernel, biological yield water use efficiency and grain yield water use efficiency average；Gck、Pck、Fck、Kck、
BWckAnd GWckA single plant's output, panicle number per plant, single fringe bear fruit grains, mass of 1000 kernel, the biology for respectively indicating control group produce
Measure the average of water use efficiency and grain yield water use efficiency.
9. a kind of paddy drought resistance evaluation side based on the comprehensive drought resistance coefficient of more gradient multiple characters according to claim 1
Method, which is characterized in that the comprehensive drought resistance coefficient of more gradient multiple characters is by T in the step (6)SDCIt indicates, calculation formula is as follows:
TSDC=logT_G(TP×TF×TK)×logT_GWTBW
In formula, T_G, TP、TF、TK, T_GW and TBWRespectively indicate a single plant's output, panicle number per plant, single fringe bear fruit grains, thousand
The T value of grain weight, biological yield water use efficiency and grain yield water use efficiency.
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