CN103004324A - Method for quickly identifying and choosing drought-resistant peanuts indoors - Google Patents
Method for quickly identifying and choosing drought-resistant peanuts indoors Download PDFInfo
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- CN103004324A CN103004324A CN2012105720902A CN201210572090A CN103004324A CN 103004324 A CN103004324 A CN 103004324A CN 2012105720902 A CN2012105720902 A CN 2012105720902A CN 201210572090 A CN201210572090 A CN 201210572090A CN 103004324 A CN103004324 A CN 103004324A
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Abstract
The invention provides a method for quickly identifying and choosing drought-resistant peanuts indoors. The method comprises the following steps of: selecting peanut seeds to be tested, and soaking the seeds; respectively cultivating and sprouting the soaked seeds in a culture dish with PEG (Polyethylene Glycol)-6000 solution with concentration of 17.5% (W/V) and 0%(W/V) for seven days in dark at 28+/-1 DEG C, wherein corresponding solution is supplemented in a quantitative manner during the cultivating and sprouting process; calculating the ratio of a germination rate, a rooting rate, a value of root length, and a value of dry weight of roots of the peanut seeds to be tested in the PEG-6000 solution with concentration of 17.5%(W/V) to those of the peanut seeds to be tested in the PEG-6000 solution with concentration of 0% (W/V); and comprehensively assessing the drought-resistant capacity of each variety of peanuts according to the number of indexes with the ratios not lower than 0.50 and the values of the ratios. The method has the advantages that the selection on the large-scale drought-resistant varieties of the peanuts can be conveniently, quickly and effectively completed; test conditions are easy to control; the method is simple and easy to implement; the period is short; the cost is low; the identifying efficiency is high; and the operability and the repeatability are high.
Description
[technical field]
The present invention relates to the screening of peanut varieties, specifically, is the method that a kind of indoor quick mirror selects the Drought-resistant peanut varieties.
[background technology]
The cycle that the arid that the pernicious variation of global climate causes occurs is shorter and shorter, and degree is more and more heavier, and production has consisted of serious threat to grain and oil crop, and the whole world accounts for 25% of global gross output because the peanut underproduction that drought causes reaches 6,000,000 t every year.China's arid and semi-arid arable area accounts for the over half of the gross area, and the peanut underproduction rate that causes because of arid is on average more than 20%.Simultaneously, arid also makes aflatoxin pollution of peanuts increase the weight of except causing the underproduction, increases the generation of damage by disease and insect.The Drought Resistance in Peanut breeding is to improve the peanut varieties drought resistance, reduce effectively approach of the loss economy brought because of arid.Peanut production is lasting, efficient, security developments is significant and the choosing of the mirror of drought resistance peanut varieties is to keeping.
Method, index, mirror choosing foundation to the choosing of peanut varieties drought resistance mirror are varied.
The most frequently used method comprises field Direct Identification method, arid canopy (or phytotron) method, potted plant (husky training, water planting, earth culture) method etc. in the method for Drought Resistance in Peanut mirror choosing, but there is respectively some shortcoming and defect in these methods: field Direct Identification method needs the field to produce and cultivation management obtains Development of Peanut information, need certain growth cycle, be subjected to the restriction of the environmental conditions such as soil, weather and affect, qualification cycle is long, labour intensity is large, land occupation and human resources; Arid canopy (or phytotron) method and potted plant (husky training, water planting, earth culture) method had both needed cultivation management, need again special moisture controlled condition and environmental facility, fund, technology, labour and resource input require huge, and its evaluation program complexity, qualification result are subjected to the restriction of Water Stress Conditions and affect; And when peanut varieties in enormous quantities or material, this several method all is subject to the restriction of the resources supplIes such as place, facility.
The index of Drought Resistance in Peanut mirror choosing is also comparatively various.Adopt the methods such as bioanalysis, Subordinate Function to carry out the Drought Resistance in Peanut characterization and evaluation, filtered out many physiological and biochemical indexs relevant with drought resistance such as blade retention ability, blade electrical conductivity, proline, malonaldehyde (MDA), peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) etc., morphological index such as stem are sturdy, internode is short, leaf narrower, thicker wax layer, powerful root system, larger root/hat compare etc. all can be used to identify the drought resistance of peanut.But lacking some can be than accurate response peanut varieties drought resistance, be suitable for giving birth to the simple index of early stage evaluation and screening.
In sum, need that a kind of program is simple, the cycle is short, efficient is high, cost is low and be suitable for giving birth to early stage evaluation and screening badly, simultaneously the drought resisting peanut varieties mirror choosing method of reliable, the good reproducibility of qualification result.But yet there are no report about this class mirror choosing method at present.
[summary of the invention]
The objective of the invention is for deficiency of the prior art, provide a kind of indoor quick mirror to select the method for Drought-resistant peanut varieties.
For achieving the above object, the technical scheme taked of the present invention is:
A kind of indoor quick mirror selects the method for Drought-resistant peanut varieties, may further comprise the steps:
1) selecting peanut variety seeds to be tried, is 17.5%(W/V respectively at the PEG-6000 solution concentration after the seed soaking) and 0%(W/V) in the culture dish, 28 ± 1 ℃ of dark cultivations were germinateed 7 days, during in time quantitatively replenish corresponding solution;
2) statistics germination rate, rooting rate, root length, root dry weight;
3) calculating described peanut variety seeds to be tried is 17.5%(W/V in the PEG-6000 solution concentration) time the long and root dry weight value of germination rate, rooting rate, root be 0%(W/V in the PEG-6000 solution concentration) time corresponding each desired value ratio, index quantity and ratio height according to its ratio 〉=0.50, the drought-resistant ability of each kind of Comprehensive Assessment, index quantity wherein 〉=0.50 the more, ratio is higher, and then peanut varieties drought-resistant ability to be tried is stronger.
Culture dish diameter described in the step 1) is 120mm, and described PEG-6000 solution is 50ml.
The basis of the germination rate step 2) is: plumule is dashed forward and is decided to be bud when breaking in the seed coat greater than 2mm and too late seed length.
Step 2) the long mensuration foundation of the root described in is: it is long that Embryo stem extension is decided to be root above seed length.
Be provided with the contrast of known Drought-resistant peanut varieties in the step 1), as the Reference cultivars of estimating peanut varieties drought-resistant ability to be tried; Preferably, described known Drought-resistant peanut varieties is educated No. 25, Ji for flower and is spent No. 2, flower to educate No. 22, Tang Ke No. 8.
The invention has the advantages that:
Indoor quick mirror of the present invention selects the method for Drought-resistant peanut varieties, can finish convenient, fast, efficiently kind in enormous quantities or carry out the indoor initial option of drought resistance, experimental condition is easy to control, method is simple, the cycle is short, cost is low, determination rates is high; The method of Identification of Drought within the vegetative period, method of the present invention can reduce labor and to the input of the facilities such as environmental resource, have stronger operability and repeatability.
[description of drawings]
Figure 1A, 1B, 1C are that each index of drought resistance kind is with the variation of coercing concentration.
Fig. 2 A, 2B, 2C are that non-each index of drought resistance kind is with the variation of coercing concentration.
[embodiment]
Below in conjunction with accompanying drawing embodiment provided by the invention is elaborated.
1 materials and methods
1.1 material
37 parts of peanut varieties, provide by Shandong Peanut Inst., be respectively: 8130, white sand 1016, G2, spend No. 14 the Shandong, rich spending No. 1, spend No. 2 the Ji, spend No. 4 the Ji, rich spending No. 6, flower is educated No. 22, flower is educated No. 28, TE-2, Datang oil, mound is spent No. 11, flower is educated No. 24, mound is spent No. 10, No. 4, Tang's oil, flower is educated No. 20, flower is educated No. 23, flower is educated No. 25, flower is educated No. 21, spend No. 8 in the Weihe River, spend No. 11 the Shandong, flower is educated No. 16, Huayu 19, No. 8, Tang Ke, mound is spent No. 13, flower is educated No. 17, flower is educated No. 27, Weihua No.6, M5,16-8, M7, mound is spent No. 12, rich spending No. 2, rich spending No. 3, mound is spent No. 9, Weihua 7.
The PEG(macrogol) molecular weight is 6000, is the very strong macromolecule organic compound of a kind of hydrophily, can produce powerful osmotic pressure after water-soluble, and its solution concentration is pressed mass volume ratio configuration (W/V).
1.2 Seed germination
Every kind is selected full, 300 in seed of the same size, putting into respectively clean disk soaks seed with distilled water, make the seed imbibition, take out the imbibition seed behind the 24h, blotting behind the surface of the seed moisture pendulum, to advance diameter be 120mm, the bottom is lined with in the culture dish of 2 metafiltration paper, 20 in every ware, the PEG-6000 solution 50ml that adds respectively each concentration, placing 28 ± 1 ℃ of dark incubators to cultivate germinates, observe every day, in time replenishes corresponding concentration solution, 7 days " Invest, Then Investigate " seed sproutings and the situation of taking root, calculate germination rate and rooting rate, the mensuration young shoot is long, plumular axis is long, root is long, simultaneously respectively with root, bud, axle, cotyledon places the measuring cup of having weighed, and 0.5h completes under 105 ℃, dry to constant weight for 80 ℃, take by weighing respectively each sample dry weight.
PEG-6000 solution concentration 0,7.5%, 12.5%, 17.5%, 22.5%5 concentration level are set, 3 repetitions.
1.3 the evaluation and screening of investigation and Drought Resistance Indexes
Plumule is prominent to break in the seed coat greater than 2mm and is decided to be bud during not as good as seed length, and measuring its length, to count bud long, as the foundation of calculating germination rate; It is long that Embryo stem extension is decided to be root above seed length, measures its axial length and root long.Following each index of statistics: bud length, root length, axial length, shoot dry matter, the rate of sprouting, root dry weight, rooting rate, calculate each index correlation coefficient and comprehensive evaluation value.
2 results and analysis
2.1 the different PEG of seed bud phase character pair coerce the reaction of concentration
Different in moisture is coerced under the concentration, it is different that each character pair of different peanut varieties seed bud phase is coerced the adaptation performance of concentration, drought resistance in the budding period between different cultivars has significant difference, and this heterogeneity of peanut varieties provides prerequisite (Fig. 1,2) for the genetic improvement of peanut varieties drought resistance.
Bud is long, axial length is different to the reaction performance that PEG coerces concentration with shoot dry matter, the dry weight of the cotyledons and 3 weight indicators of root dry weight with long 3 length index of root, root length and bud personal attendant coerce Enrichment and reduce gradually, and the axial length reduction is not obvious, even increases (Figure 1A, Fig. 2 A) to some extent; Root dry weight and shoot dry matter reduce gradually with coercing Enrichment, and the dry weight of the cotyledons then changes not quite, even increase (Figure 1B, Fig. 2 B); Germination rate and rooting rate two indexes are consistent to the reaction performance that PEG coerces concentration, all are and reduce gradually trend (Fig. 1 C, Fig. 2 C) with coercing Enrichment.
Its root length of kind that drought resistance is stronger is stronger to the adaptability of water stress concentration, and 22.5% still has root growth when coercing concentration, and the weak kind of drought resistance is low when coercing concentration, the aobvious reduction kept burning day and night of its root; Flower is educated No. 27, Shandong and is spent No. 14,8130, Ji to spend No. 2, flower to educate No. 22, No. 8, Tang Ke and flower to educate rooting rate and the root dry weight of No. 25 kinds and all show coercing the insensitive of concentration, still keeping growth up to 22.5% the time, show that they are stronger to the tolerance of drought stress, have higher drought-enduring, drought resistance.Germination rate is comparatively directly perceived and obvious to coercing concentration-response, under low concentration, No. 4, Tang oil, flower educate No. 16, Huayu 19, mound spend No. 12, M5,16-8, M7, mound spend No. 12, flower educate No. 17, rich spend No. 2, rich spend No. 3, mound spend No. 9, Weihua 7, rich spend No. 6, mound spend 11 and germination rate, axial length, the shoot dry matter equivalence of No. 24 kinds of Hua Yu all be lower than 50%, show that the germinating capacity of these kinds is subjected to the water stress concentration affects larger; 8130, the Ji spends No. 2, flower to educate No. 22, flower to educate the then less concentration as influencing factor of being coerced of germination rate, shoot dry matter that No. 25, flower educate No. 28 kinds.
2.2 suitable PEG coerces the establishment of concentration
Different peanut varieties is different to the reaction of coercing concentration, when coercing concentration when being lower than 7.5%, most each proterties of variety seeds germination period all do not show obvious reaction, but when coercing concentration when being increased to 22.5%, most kind bud phases, each characteristic index value all obviously was suppressed.PEG-6000 solution coerce concentration 17.5% time, root dry weight, rooting rate, the kind of the ratio of germination rate 〉=0.50 has flower to educate No. 25, flower is educated No. 27,8130, spend No. 2 the Ji, spend No. 4 the Ji, flower is educated No. 22, No. 8 kinds of Tang Ke, show that its drought resistance is stronger, and Huayu 19, mound is spent No. 13, No. 4, Tang's oil, white sand 1016, G2, spend No. 14 the Shandong, rich spending No. 1, rich spending No. 6, flower is educated No. 28, TE-2, Datang oil, mound is spent No. 11, flower is educated No. 24, mound is spent No. 10, No. 4, Tang's oil, flower is educated No. 20, flower is educated No. 23, flower is educated No. 21, spend No. 8 in the Weihe River, spend No. 11 the Shandong, flower is educated No. 16, Huayu 19, mound is spent No. 13, flower is educated No. 17, Weihua No.6, M5,16-8, M7, mound spends No. 12 kinds its ratio when low concentration lower, show that its drought-resistant ability a little less than.When coercing concentration when being 17.5%, there is 77.8% kind rooting rate to be suppressed, when concentration was higher than 22.5%, nearly 90% kind can not be germinateed.Analysing Root Characters is subjected to the impact of hypertonic solution concentration and kind different, 17.5% coerce the suitable concentration that concentration is root length, rooting rate, root dry weight index screening, exist larger difference between the root length of tested variety, rooting rate, germination rate, each indicator ratio of root dry weight, this coerces concentration and can be used as the suitable concentration that peanut variety seeds germination period screening of drought resistance is identified.Caused under 17.5% Simulated Water potential condition and coerced inhibition to what the part variety seeds was sprouted, this coerces suitable concentration (Figure 1A-2C) that concentration can be used as bud phase Screening and Identification drought resistance kind.
2.3 the Screening and Identification of each kind bud phase Drought Resistance Indexes
Coerce Germinating Seed of Peanut by hypertonic solution, select suitable to coerce concentration and observation index is the prerequisite of Drought Resistance of Variety evaluation and screening.Under the water stress environment, germination rate, the germination vigor of seed all reduce, and the growth of radicle and plumule is suppressed, and the different cultivars difference is remarkable, and the suffered impact of the kind of strong drought resistance is starkly lower than the weak kind of drought resistance.Correlation coefficient between each proterties of peanut sprout phase sees Table 1.
Correlation coefficient between each proterties of table 1 peanut sprout phase
Annotate: it is 0.05 and 0.01 that " * ", " * * " represent respectively significance.
Significance of correlation coefficient shows between each index, all exist either large or small correlation between all indexs, wherein root long with the indexs such as axial length, the rate of sprouting, root dry weight, rooting rate between all exist significantly or extremely significant dependency relation, between shoot dry matter and any index all without dependency relation.Correlation coefficient between root length, root dry weight, rooting rate and comprehensive evaluation value is the highest, be respectively 0.8842,0.8161 and 0.7435, illustrate that root length, root dry weight, rooting rate, the rate of sprouting etc. all can reflect the Comprehensive drought resistance of Germinating Peanut Seeds phase preferably, germination period relative germination rate, rooting rate, root length, root dry weight all can be used as the reliability index of peanut budding period Identification of Drought screening.
2.4 drought resistance kind mirror selects establishment of standard
Based on the above results: PEG-6000 solution coerce concentration 17.5% time, each index relative value of each drought resistance kind is general 〉=0.50, and each index relative value of each non-drought resistance kind general＜0.05; Germination period relative germination rate, rooting rate, root length, root dry weight can be used as the reliability index of peanut budding period Identification of Drought screening simultaneously.Therefore, drought resistance kind mirror selects standard to be: calculating each kind (being) is 17.5%(W/V in the PEG-6000 solution concentration) time long and each index relative value of root dry weight of germination rate, rooting rate, the root ratio of corresponding each desired value of contrast (each desired value with), index quantity and ratio height according to its ratio 〉=0.50, the drought-resistant ability of each kind of Comprehensive Assessment, the index quantity of certain kind 〉=0.50 the more, ratio is higher, and the drought-resistant ability of this kind is stronger.
Repeat above-mentioned experimentation 5 times, conclusion is identical.
Use method of the present invention peanut varieties is carried out drought resistance mirror choosing, mirror is selected flower and is educated No. 25, flower and educate No. 27,8130, Ji and spend No. 2, flower to educate No. 22, No. 86 peanut varieties of Tang Ke to have stronger drought resistance.In the demonstration test of the potted plant and arid canopy cultivation of the water stress under the controlled condition of carrying out, by the investigation and analysis of plant growth and development state and yield index in vegetative period, use method of the present invention to the qualification result of Drought-resistant peanut varieties and peanut growth in the phase Identification of Drought result's identical rate reach more than 89%.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the inventive method; can also make some improvement and replenish, these improvement and replenish and also should be considered as protection scope of the present invention.
Claims (6)
1. an indoor quick mirror selects the method for Drought-resistant peanut varieties, it is characterized in that, may further comprise the steps:
1) selecting peanut variety seeds to be tried, is 17.5%(W/V respectively at the PEG-6000 solution concentration after the seed soaking) and 0%(W/V) in the culture dish, 28 ± 1 ℃ of dark cultivations were germinateed 7 days, during in time quantitatively replenish corresponding solution;
2) statistics germination rate, rooting rate, root length, root dry weight;
3) calculating described peanut variety seeds to be tried is 17.5%(W/V in the PEG-6000 solution concentration) time the long and root dry weight value of germination rate, rooting rate, root be 0%(W/V in the PEG-6000 solution concentration) time corresponding each desired value ratio, index quantity and ratio height according to its ratio 〉=0.50, the drought-resistant ability of each kind of Comprehensive Assessment, index quantity wherein 〉=0.50 the more, ratio is higher, and then peanut varieties drought-resistant ability to be tried is stronger.
2. method according to claim 1 is characterized in that, the culture dish diameter described in the step 1) is 120mm, and described PEG-6000 solution is 50ml.
3. method according to claim 1 is characterized in that step 2) described in the basis of germination rate be: plumule is prominent to break in the seed coat greater than 2mm and is decided to be bud during not as good as seed length.
4. method according to claim 1 is characterized in that step 2) described in the long mensuration of root according to being: Embryo stem extension surpasses seed length, and to be decided to be root long.
5. method according to claim 1 is characterized in that, is provided with the contrast of known Drought-resistant peanut varieties in the step 1), as the Reference cultivars of estimating peanut varieties drought-resistant ability to be tried.
6. method according to claim 5 is characterized in that, described known Drought-resistant peanut varieties is that No. 2 and Tang Ke are spent No. 8 in the Ji.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238449A (en) * | 2013-05-24 | 2013-08-14 | 山东省花生研究所 | Method for rapidly identifying and selecting salt-tolerant peanut varieties in room |
| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105875370A (en) * | 2014-12-12 | 2016-08-24 | 时洋洋 | Cultivation method of peanut sprouts |
| CN112673750A (en) * | 2020-12-30 | 2021-04-20 | 云南中烟工业有限责任公司 | Method for rapidly screening stress resistance of plants |
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| CN1582636A (en) * | 2004-06-04 | 2005-02-23 | 广东省农业科学院作物研究所 | Method for breeding drought lolerant peanut |
| WO2011119681A1 (en) * | 2010-03-23 | 2011-09-29 | Crop Microclimate Management Inc. | Methods for increasing tolerance to abiotic stress in plants |
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2012
- 2012-12-24 CN CN2012105720902A patent/CN103004324A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582636A (en) * | 2004-06-04 | 2005-02-23 | 广东省农业科学院作物研究所 | Method for breeding drought lolerant peanut |
| WO2011119681A1 (en) * | 2010-03-23 | 2011-09-29 | Crop Microclimate Management Inc. | Methods for increasing tolerance to abiotic stress in plants |
Non-Patent Citations (1)
| Title |
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| 张智猛 等: "花生品种芽期抗旱性指标筛选与综合性评价", 《中国农业科技导报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238449A (en) * | 2013-05-24 | 2013-08-14 | 山东省花生研究所 | Method for rapidly identifying and selecting salt-tolerant peanut varieties in room |
| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105875370A (en) * | 2014-12-12 | 2016-08-24 | 时洋洋 | Cultivation method of peanut sprouts |
| CN112673750A (en) * | 2020-12-30 | 2021-04-20 | 云南中烟工业有限责任公司 | Method for rapidly screening stress resistance of plants |
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Application publication date: 20130403 |