CN112544372A - Method for identifying drought resistance of root system of winter wheat - Google Patents
Method for identifying drought resistance of root system of winter wheat Download PDFInfo
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- CN112544372A CN112544372A CN202110030446.9A CN202110030446A CN112544372A CN 112544372 A CN112544372 A CN 112544372A CN 202110030446 A CN202110030446 A CN 202110030446A CN 112544372 A CN112544372 A CN 112544372A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a method for identifying the root cap specific drought resistance of winter wheat, which comprises the following steps: s1, respectively arranging a water treatment group and a drought treatment group under the simulated field environment condition of the root system observation room, wherein the water treatment group fills 2 spring water and fills the joints, the spikes and the flowers respectively, and the filling amount is 603Per mu; the drought treatment group does not irrigate on the basis of full seedling emergence; s2, respectively measuring the above-ground biomass dry weight and the whole root system dry weight in the jointing stage and the heading and flowering stage, calculating the root-crown ratio, and then calculating the root-crown ratio drought resistance index RSRDI through the following formula: RSRDI = rsrs.t2·RSRS.W‑1·RSRCK.W·(RSRCK.T2) -1; and S3, screening drought-resistant varieties based on the root-cap ratio drought-resistant index RSRDI. The invention utilizes the root cap ratio drought resistance index to carry out screening, can ensure the screening accuracy, can reduce the screening time, and provides a new method for identifying the drought resistance of the winter wheat.
Description
Technical Field
The invention relates to a method for identifying drought resistance of wheat, in particular to a method for identifying drought resistance of winter wheat.
Background
Drought and rain are natural characteristics of wheat areas in Huang-Huai (northern China), and the climate drought and water resource shortage become main factors limiting wheat production, so that the water-saving drought resistance of wheat becomes a hotspot of current research. The root system is an important organ of wheat life activities, mainly plays a role in absorbing nutrients and water, participating in synthesis and transformation of substances in vivo and the like, has close relation with drought resistance and growth of plants and formation of yield, plays an important role in growth and development, physiological functions and substance metabolism processes of wheat, and is an important component part for analysis and research on crop drought resistance and water conservation.
Disclosure of Invention
The invention aims to provide a method for identifying the drought resistance of winter wheat, which realizes the rapid and accurate identification of the drought resistance of the winter wheat based on the root cap ratio drought resistance index.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for identifying the root cap specific drought resistance of winter wheat comprises the following steps:
s1, respectively arranging a water treatment group and a drought treatment group under the simulated field environment condition of the root system observation room, wherein the water treatment group fills 2 spring water and fills the joints, the spikes and the flowers respectively, and the filling amount is 603Per mu; the drought treatment group does not irrigate on the basis of full seedling emergence;
s2, respectively measuring the above-ground biomass dry weight and the whole root system dry weight in the jointing stage and the heading and flowering stage, calculating the root-crown ratio, and calculating the root-crown ratio drought resistance index RSRDI through the formula (1):
RSRDI=RSRS.T2·RSRS.W-1·RSRCK.W·(RSRCK.T2)-1 （1）
wherein, RSRDI is a root-crown ratio drought resistance index, RSRS.T is a stress treatment root-crown ratio of the material to be detected, RSRS.W is a comparison treatment root-crown ratio of the material to be detected, RSRCK.W is a comparison variety comparison treatment root-crown ratio, and RSRCK.T is a comparison variety stress treatment root-crown ratio;
s3, screening drought-resistant varieties based on the root-crown ratio drought-resistant index RSRDI and the following standards:
the root crown ratio drought resistance index is more than or equal to 1.3, namely a new variety with extremely strong drought resistance;
the root crown ratio drought resistance index is 1.1-1.3, namely a new variety with strong drought resistance;
the root crown ratio drought resistance index is 0.9-1.1, namely a new variety with medium drought resistance;
the root cap ratio drought resistance index is 0.7-0.9, namely the new variety with weak drought resistance;
the root cap ratio drought resistance index is less than or equal to 0.7, namely the new variety with poor drought resistance.
The invention utilizes the root cap ratio drought resistance index to carry out screening, can ensure the screening accuracy, can reduce the screening time, and provides a new method for identifying the drought resistance of the winter wheat.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Examples
In order to realize a quick and accurate identification and screening method, the applicant finally determines to use the root-crown ratio drought resistance index for screening through repeated experiments by testing different water treatment and different repeated parameters of 5 varieties (respectively, the weighbridge 9966, the weighbridge 11-6021, the weighbridge S29, the Jimai 22, the weighbridge 4399 and the weighbridge 4399 are controls), so that the screening accuracy can be ensured, and the screening time can be shortened.
The experimental method comprises the following steps:
(1) preparing a root observation room for planting wheat; 60 PVC pipes with the length of 2.4m and the diameter of 0.11 m; washing a water pool of a wheat root system, taking plough layer soil from a field, and sieving to remove grass roots and impurities for later use;
(2) calculating the soil weight in the pipe according to the volume weight of the soil, calculating the length of the pipe of the injected water according to the field water capacity and the soil water content, and calculating the length of the pipe of the injected water according to the volume weight of the soil (1.3 g/cm)3) And calculating the weight of soil in the pipe:
3.14 × 5.252 × 2.36 × 1.3=26.6 kg
Calculating the water amount to be poured according to the field water capacity (28%) and the soil water content at that time: 26.6 x (28% -20.8%) =1.9152 kg
And (2) pouring the soil screened in the step (1) into the pipe, wherein the soil is poured while water is poured, a small amount of water is poured for many times to facilitate water permeation, and the bottom of the pipe is supported by a flowerpot to prevent water and soil loss.
(3) The test adopts sequential arrangement, 3 plants are planted in each tube, the weight of soil in the tube is calculated according to the volume weight of the soil, the water injection amount is calculated according to the field water capacity and the soil water content, compaction seeding is carried out, the wheat variety balance 4399 is set as a reference variety, and under the condition of simulating the field environment in a root observation room, water treatment (2 water for spring irrigation, water for jointing and heading, and the water injection amount is 60 percent)3Per mu) and drought treatment (no irrigation on the basis of full seedling).
(4) Respectively measuring the above-ground biomass dry weight and the dry weight of the wheat root system by sectional sampling in the jointing stage and the heading and flowering stage, summing to obtain the dry weight of the whole root system, calculating the root-crown ratio, and calculating the drought resistance index of the root-crown ratio by the formula (1):
RSRDI=RSRS.T2·RSRS.W-1·RSRCK.W·(RSRCK.T2)-1 （1）
wherein, RSRDI is a root-cap ratio drought resistance index, RSRS.T is a stress treatment root-cap ratio of the material to be detected, RSRS.W is a comparison treatment root-cap ratio of the material to be detected, RSRC.W is a comparison variety comparison treatment root-cap ratio, and RSRC.T is a comparison variety stress treatment root-cap ratio.
(5) Screening of drought-resistant varieties is realized based on root-crown ratio drought-resistant index RSRDI and the grading standard of Table 1, and the results are shown in tables 2, 3, 4 and 5.
TABLE 1 wheat root-crown ratio drought resistance index RSRDI grading Standard
TABLE 2 root-crown ratio character table of wheat in jointing stage
TABLE 3 drought resistance results during wheat jointing stage
TABLE 4 root-crown ratio of wheat at heading stage
TABLE 5 wheat heading stage drought resistance results
The experimental results are as follows: the drought resistance results of the scale 9966, the scale H11-6021, the scale S29 and the Jimai 22 in the jointing stage are respectively medium, strong, medium and strong; the drought resistance results in heading period are strong, strong and medium respectively, and completely accord with the drought resistance obtained by earlier research.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (1)
1. A method for identifying the root cap ratio drought resistance of winter wheat is characterized by comprising the following steps: the method comprises the following steps:
s1, respectively arranging a water treatment group and a drought treatment group under the simulated field environment condition of the root system observation room, wherein the water treatment group fills 2 spring water and fills the joints, the spikes and the flowers respectively, and the filling amount is 603Per mu; the drought treatment group does not irrigate on the basis of full seedling emergence;
s2, respectively measuring the above-ground biomass dry weight and the whole root system dry weight in the jointing stage and the heading and flowering stage, calculating the root-crown ratio, and calculating the root-crown ratio drought resistance index RSRDI through the formula (1):
RSRDI=RSRS.T2·RSRS.W-1·RSRCK.W·(RSRCK.T2)-1 （1）
wherein, RSRDI is a root-crown ratio drought resistance index, RSRS.T is a stress treatment root-crown ratio of the material to be detected, RSRS.W is a comparison treatment root-crown ratio of the material to be detected, RSRCK.W is a comparison variety comparison treatment root-crown ratio, and RSRCK.T is a comparison variety stress treatment root-crown ratio;
s3, screening drought-resistant varieties based on the root-crown ratio drought-resistant index RSRDI and the following standards:
the root crown ratio drought resistance index is more than or equal to 1.3, namely a new variety with extremely strong drought resistance;
the root crown ratio drought resistance index is 1.1-1.3, namely a new variety with strong drought resistance;
the root crown ratio drought resistance index is 0.9-1.1, namely a new variety with medium drought resistance;
the root cap ratio drought resistance index is 0.7-0.9, namely the new variety with weak drought resistance;
the root cap ratio drought resistance index is less than or equal to 0.7, namely the new variety with poor drought resistance.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170669A (en) * | 2021-05-10 | 2021-07-27 | 河北省农林科学院旱作农业研究所 | Pre-identification and selection method of wheat material with high water-saving index |
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| UA48032U (en) * | 2009-02-26 | 2010-03-10 | Институт Физиологии Растений И Генетики Нан Украины | Method for assessing drought resistance of winter wheat |
| CN104838905A (en) * | 2015-04-13 | 2015-08-19 | 西北农林科技大学 | Method of evaluating watermelon seedling-stage drought resistance |
| CN109729777A (en) * | 2018-12-18 | 2019-05-10 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | It is a kind of high throughput Maize Seedling drought tolerance identification and evaluation method |
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- 2021-01-11 CN CN202110030446.9A patent/CN112544372A/en active Pending
Patent Citations (4)
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| UA48032U (en) * | 2009-02-26 | 2010-03-10 | Институт Физиологии Растений И Генетики Нан Украины | Method for assessing drought resistance of winter wheat |
| CN104838905A (en) * | 2015-04-13 | 2015-08-19 | 西北农林科技大学 | Method of evaluating watermelon seedling-stage drought resistance |
| CN109729777A (en) * | 2018-12-18 | 2019-05-10 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | It is a kind of high throughput Maize Seedling drought tolerance identification and evaluation method |
| CN111480476A (en) * | 2020-03-23 | 2020-08-04 | 安徽徽农生态食品有限公司 | Comprehensive evaluation method for identifying drought tolerance of coix seed germplasm resources |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170669A (en) * | 2021-05-10 | 2021-07-27 | 河北省农林科学院旱作农业研究所 | Pre-identification and selection method of wheat material with high water-saving index |
| CN113170669B (en) * | 2021-05-10 | 2022-12-16 | 河北省农林科学院旱作农业研究所 | Pre-identification and selection method of wheat material with high water-saving index |
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