CN106275862B - Portable fruit tree germplasm resources collection bag - Google Patents
Portable fruit tree germplasm resources collection bag Download PDFInfo
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- CN106275862B CN106275862B CN201610892117.4A CN201610892117A CN106275862B CN 106275862 B CN106275862 B CN 106275862B CN 201610892117 A CN201610892117 A CN 201610892117A CN 106275862 B CN106275862 B CN 106275862B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D33/00—Details of, or accessories for, sacks or bags
- B65D33/16—End- or aperture-closing arrangements or devices
- B65D33/28—Strings or strip-like closures, i.e. draw closures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
- B65D81/26—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators
- B65D81/264—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators for absorbing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3888—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation wrappers or flexible containers, e.g. pouches, bags
- B65D81/3897—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation wrappers or flexible containers, e.g. pouches, bags formed of different materials, e.g. laminated or foam filling between walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
- B65D85/52—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage for living plants; for growing bulbs
Abstract
The present invention relates to a kind of portable Fruit Tree Germplasm Resources collection bag, the upper and lower of its Tarpauling base wad is trapezoidal spademan, and side is connected with heat-insulation and heat-preservation pad, and two spademans are equipped with packing hole, provided with transparent bag, pouch and a pair from patch band, the back side is provided with a pair from patch band in front.The front of collection of resources pad is provided with a pair from patch band, hides lid along to be flexible thereon, lower edge is provided with flexible water storage bag, and reverse side is provided with a pair from patch band, should mutually pasted from patch band Corresponding matching with the back side of Tarpauling base wad from patch band.Branch unit bag includes bag body, and the side of bag body is pasted by a pair from patch band and collection of resources pad are positive from patch band；Arch support is provided with each bag body, bottom is furnished with absorbent cloth band.Branch unit bag is pasted on collection of resources pad, and its absorbent cloth band is placed in flexible water storage bag, is furnished with temperature-humidity monitor in transparent bag, and the probe of temperature-humidity monitor is placed in a sampling branch unit bag.
Description
Technical field
The present invention relates to a kind of spectacle case of Fruit Tree Germplasm Resources collection branch, especially a kind of portable fruit tree germplasm money
Source collection bag, a kind of germ plasm resource for belonging to cultivation of fruit tree thremmatology field collect specific purpose tool.
Background technology
China has abundant Fruit Tree Germplasm Resources.[river is with literary is perennial and asexually propagated crop kind for Ju incomplete statistics
Matter resource-sharing research [M] Beijing:Chinese agriculture publishing house, 2006:77-86], 45 kinds of whole world main fruit trees crop species
Contain 3893 botany kinds, originate from China for 725 kinds, account for the world 18.62%；Apple, pears, grape, peach, Lee, apricot,
The wild species quantity of large fruit tree origin China such as jujube, walnut, citrus, loquat, longan, lichee accounts for the 56.13% of the world；45 kinds
Main fruit trees crop species cultigen has 15 kinds of origins to originate from China or part in China, and accounting for 33%, [Yu De dredges deciduous fruit trees
Taxology [M] Shanghai:Science and Technology of Shanghai publishing house, 1984].Jiangsu Province Agriculture Science Institute Horticultural Research Institute is mainly engaged in peach, grass
Collection, preservation, characterization and evaluation and the utilization of the Fruit Tree Germplasm Resources such as the certain kind of berries, grape, pears, wild mountain a surname walnut and breeding of new variety are ground
Study carefully, now built up national peach, strawberry germplasm garden and Jiangsu Province's Fruit Tree Germplasm Resources gene pool, peach is have collected from both at home and abroad
More than 800 parts, more than 600 parts of strawberry, more than 260 parts of pears, more than 100 parts of grape, the germplasm such as more than 60 parts of wild mountain a surname walnut.
The Fruit Tree Germplasm Resources such as peach, grape, pears, wild mountain a surname walnut, which are introduced a fine variety, all uses asexual reproduction method, is gathered from strange land
Bud, which is remarried, to be connected on the high stock of compatibility, and this method can not only keep the hereditary capacity of female parent, also keeps intrinsic excellent of kind
Benign shape, secondly for the fruit tree of vegetative propagation without juvenile phase, beginning fruiting period is early, and the seedling of vegetative propagation does not have a segregation phenomenon, and nursery stock is neat
Unanimously.
Fruit Tree Germplasm Resources acquisition time be able to will typically be carried out throughout the year.Kind is collected in traditional fruit tree field at present
Matter, all using the parcel scion such as wet newspaper, coil paper, wet towel, then in outer one polybag of surface cover, winter is in this way
It is feasible, but can only see bare branch, other important fruit properties, leaf characters and disease resistances during winter field collection germplasm
It all can't see, what germplasm do not gathered it is difficult to debate, therefore, optimal acquisition period is Growth status developmental stage, has leaf on tree, has
Fruit, moreover it is possible to which the resistance of observation of plant, i.e., the 7-9 months are optimal, and this stage Lingao temperature, and collection scion skill bar is mostly wooden in half
Change, be not easy to store, moisture content has lacked branch can withered, and the more buds of moisture content easily rot, and in addition temperature height, branch easily brown stain, loses work
Power, it is extremely low to gather the branch graft survival rate returned.In view of the above-mentioned problems, this area is highly desirable to obtain a kind of new fruit tree open country
Outer germ plasm resource collects bag.
The content of the invention
It is an object of the present invention to for the adverse effect that collection environment comes to graft strip at present, current usual strick precaution
Measure operation is more complicated, and effect is also not fully up to expectations, there is provided a kind of brand-new portable Fruit Tree Resources collection bag.
The object of the present invention is achieved like this：A kind of portable Fruit Tree Germplasm Resources collection bag, including Tarpauling base
Pad, it is characterised in that：
A) form trapezoidal spademan after the upper following extension of the Tarpauling base wad, the side of Tarpauling base wad with it is wide
Heat-insulation and heat-preservation pad be connected as a single entity, is provided with symmetrical packing hole in the drift angle of upper and lower spademan, and be furnished with tie string；Tarpauling base
Transparent bag, pouch and a pair of parallel from patch band are provided with the front of pad, is extended from patch band by Tarpauling base wad
Outer end edge is padded to heat-insulation and heat-preservation；The back side of Tarpauling base wad be provided with a pair of parallel insertion Tarpauling base wad and every
Hot heat keeper from patch band, should mutually be pasted with Corresponding matching from patch from patch band and Tarpauling base wad are positive；
B) collection of resources pad is cotton pad, and the front of collection of resources pad is provided with a pair of parallel from patch band, collection of resources
The lower edge of pad is provided with flexible water storage bag；The reverse side of collection of resources pad be provided with it is a pair of parallel from patch band, reverse side from patch
Mutually pasted with insertion collection of resources pad and with the back side of Tarpauling base wad from patch band Corresponding matching；
C) branch unit bag includes bag body, and the side of bag body is provided with a pair from patch band, bag body from patch band and resource
Collection pad is positive mutually to be pasted from patch band Corresponding matching；Also at least provided with three arch supports in each bag body, they divide
Cloth the top of bag body, in, bottom, the bottom of each bag body is furnished with absorbent cloth band；
D) at least paste has a branch unit bag, the absorbent cloth bottom of each branch unit bag on the collection of resources pad described in
The absorbent cloth band in portion is placed in the flexible water storage bag of collection of resources rim strip；The transparent bag of the Tarpauling base wad is equipped with temperature
Moisture monotor, the probe of temperature-humidity monitor are placed in one through the Tarpauling base wad in transparent bag and randomly selected adopted
In sample branch unit bag.
In the present invention：Described heat-insulation and heat-preservation pad is the complex of cotton lining and double-sided aluminum foil bubble protection against the tide heat keeper；
The bag body of the branch unit bag is that double-sided aluminum foil bubble protection against the tide heat keeper is lined with cotton liner, the suction of cotton liner and bottom
Water cloth band connection, the coverage mouth of air-vent is additionally provided with the top of branch unit bag；Described flexible water storage bag is double-sided aluminum foil gas
" U " aqua storage tank that moistureproof heat keeper makes is steeped, pasting certainly by matching between the wall outside of " U " aqua storage tank and collection of resources pad
Cloth band connection.
In the present invention：It is furnished with water-absorbing sponge in the flexible water storage bag of collection of resources rim strip；Equipped with storage on heat-insulation and heat-preservation pad
Ice bag is hidden, the one side for storing ice bag is provided with from patch band, stores being extended to from patch band and Tarpauling base wad front for ice bag
On heat-insulation and heat-preservation pad stickup is mutually matched from patch band.
In the present invention：The height of each branch unit bag is 30-38cm, the span 3-5cm of arch support；Described money
Source collection pad is upper can to paste the individual parallel branch unit bags of 5-15, interval 1cm during paste between adjacent branch unit bag.
The advantage of the invention is that：Gathered and wrapped using portable Fruit Tree Resources, be adapted to go field acquisition resource throughout the year,
And every part of germ plasm resource all independently dispenses, and ensure that and introduces a fine variety accuracy；Scion, portable Fruit Tree Resources are gathered in summer and autumn high temperature
Bag scion bud vigor is gathered, grafting Cheng Li is remarkably improved, ensure that successful introduction rate.
Experiment shows that branch thick and panel length have notable difference between pears, apple, peach, wild mountain a surname walnut, same
There is also difference, central annual young sprout rugosity it is carefully most ' rain liquid distilled from honeysuckle flowers or lotus leaves ' peach between fruit trees different cultivars, is 0.32cm, most slightly
Be ' Shi Lai ' wild mountain a surname walnut, be 1.24cm, between differ 0.82cm；It is ' Nagafu No.2 ' apple that panel length is most short, is
2.11cm, most long is ' dragon's fountain shortcake pears ', is 4.82cm, between differ 2.71cm.Therefore collect 10 with every part of resource
Bud, 2 branches are cut, collect small bag length at least 30cm, width at least 3cm.Determine therefrom that the height of each branch unit bag 10
Spend for 30-38cm, the span 3-5cm of arch support, 5-15 parallel branch units can be pasted on each collection of resources pad
Bag 10.
It is also an advantage of the present invention that using the portable fruit tree germplasm collection bag of the present invention in the outdoor highest temperature 38.8
DEG C, temperature is only 12.9 DEG C in bag, from 8:00-18:Between 00, temperature is maintained at 2.3-17.9 DEG C in bag.Scion electrical conductivity, third
The significantly high experimental group of dialdehyde content control group, illustrate experimental group scion cell leakage and be not affected by destruction.By this technology
Link makes field grafting survival power improve 62% than control group, illustrates that the technology not only ensures to introduce a fine variety purity, success rate, also saved
A large amount of human and material resources and quality time are saved, more preferably sound assurance is provided for resource collection work.
Brief description of the drawings
Fig. 1：It is the positive structure schematic of the embodiment of the present invention；
Fig. 2：It is the reverse structure schematic of the embodiment of the present invention；
Fig. 3：It is branch unit bag arrangement mode schematic diagram on collection of resources pad in the embodiment of the present invention.
In figure：1st, heat-insulation and heat-preservation pad, 2, Tarpauling base wad, 3, from viscose cloth tape with the public piece hooked, 4, transparent bag, 5,
Temperature-humidity monitor, 6, kit bag, 7, pen bag, 8, storage ice bag, 9, the master slice with suede from viscose cloth tape, 10, collection of resources pad,
11st, flexible water storage bag, 12, lead, 13, tie string, 14, it is flexible hide lid, 15, branch unit bag, 16, arch support, 17, inhale
Water cloth band.
Embodiment
The nonrestrictive concrete structure for disclosing the embodiment of the present invention of accompanying drawing, makees to the present invention into one below in conjunction with the accompanying drawings
The description of step ground.
Embodiment 1
From Fig. 1 and Fig. 2, the present invention includes Tarpauling base wad 2, the upper following extension of the Tarpauling base wad 2
After form trapezoidal spademan, the side of Tarpauling base wad 2 is connected as a single entity with wide heat-insulation and heat-preservation pad 1, the drift angle of upper and lower spademan
It is interior to be provided with symmetrical packing hole, and it is furnished with tie string 13.
In Fig. 1, transparent bag 4, pouch and a pair of parallel from patch are provided with the front of Tarpauling base wad 2
Band, its end edge is extended to through heat-insulation and heat-preservation pad 1 by Tarpauling base wad 2 from patch band, wherein, pouch includes kit bag
6 and pen bag 7, it is that band hooks public piece 3 (can also select band suede master slice 9) from patch band from patch band.It is furnished with heat-insulation and heat-preservation pad 1
Store ice bag 8, the one side of storage ice bag 8 is provided with from patch band, when Tarpauling base wad 2 it is positive from patch band be from patch
When band hooks public piece 3 in band, storage ice bag 8 can only select matched band suede master slice 9 from patch band.
In fig. 2, the back side of Tarpauling base wad 2 is provided with a pair of parallel insertion Tarpauling base wad 2 and heat-insulation and heat-preservation
Pad 1 from patch band, from the band suede master slice 9 of the selection of patch band and the pairing of the front of Tarpauling base wad 2 (when Tarpauling base wad 2
Positive is when selecting band suede master slice 9, and its band matched can also be selected to hook public piece 3), can be with waterproof from patch band in use, being somebody's turn to do
Canvas base wad 2 is positive mutually to be pasted from the Corresponding matching of patch band 3, when so may insure that heat-insulation and heat-preservation pad 1 is rolled, the back side with
It is positive mutually to be pasted from patch band.
In fig. 2, collection of resources pad 10 is cotton pad, and the front of collection of resources pad 10 is provided with a pair of parallel from patch band
3, it is that band hooks public piece 3 (can also select band suede master slice 9) from patch band from patch band, the upper edge of collection of resources pad 10 is flexibility
Lid 14 is hidden, lower edge is provided with flexible water storage bag 11, and the reverse side of collection of resources pad 10 is provided with a pair of parallel from patch band, waterproof sail
Cloth base wad 2 is band suede master slice 9 from patch band, and the net of collection of resources pad reverse side can only select matched band to hook from patch band
Public piece 3.
Fig. 2 combinations Fig. 3 is visible, and branch unit bag 15 includes bag body, and the one side of bag body is provided with a pair from patch band, bag body
Mutually pasted from patch band Corresponding matching from patch band and collection of resources pad 10 are positive；In each branch unit bag 15 also at least
Provided with three arch supports 16, they be distributed in bag body top, in, bottom, the bottom of each bag body is furnished with absorbent cloth band 17.Often
The interval 1cm between the adjacent branch unit bag 15 of 5-15 branch unit bag 15 can be pasted on individual collection of resources pad 10, often
The absorbent cloth band 17 of the individual bottom of branch unit bag 15 is placed in the flexible water storage bag 11 of collection of resources rim strip 10；The waterproof sail
The transparent bag of cloth base wad 2 is furnished with temperature-humidity monitor 5, the waterproof sail of the probe of temperature-humidity monitor 5 through in transparent bag 4
Cloth base wad 2 is placed in a randomly selected sampling branch unit bag 15 by lead 12.
In the present embodiment, described heat-insulation and heat-preservation pad 1 be cotton lining and double-sided aluminum foil bubble protection against the tide heat keeper it is compound
Body.The bag body of the branch unit bag 15 is that double-sided aluminum foil bubble protection against the tide heat keeper is lined with cotton liner, cotton liner and bottom
The absorbent cloth band 17 in portion connects.Described flexible water storage bag 11 is " U " aqua storage tank that double-sided aluminum foil bubble protection against the tide heat keeper makes,
Matched somebody with somebody on the outside of one wall of " U " aqua storage tank between collection of resources pad 10 by matching from patch band connection, flexible water storage bag 11
There is water-absorbing sponge.
In the present embodiment, in order to improve the effect of cooling moisture-retaining, the front of collection of resources rim strip 10 can also configure storage
Ice bag 8.
Embodiment 2：
The size of branch unit bag 15 determines
Experiment is in Jiangsu Province agricultural Ke Yuan theatre Germplasm Resources, national peach Germplasm Resources, academy of agricultural sciences six directions animals and plants
Proving ground, Xuzhou Dasha River orchard are acquired investigation.Resource selects the peripheral long shoot of the tree vigo(u)r golden mean of the Confucian school, robust growth, by length
Divided by joint number calculates panel length, rugosity oil ga(u)ge kind of calliper, per 3 repetitions of kind, repeats 10 branches every time.
As shown in Table 1, between different fruit trees there are notable difference, same fruit trees difference product in branch thick and panel length
There is also difference between kind；Pears, apple, peach, annual young sprout rugosity is carefully most ' rain liquid distilled from honeysuckle flowers or lotus leaves ' peach among wild mountain a surname walnut, is
0.32cm, most thick is ' Shi Lai ' wild mountain a surname walnut, is 1.24cm, between differ 0.82cm；Panel length is most short for ' long richness 2
Number ' apple, be 2.11cm, most long is ' dragon's fountain shortcake pears ', is 4.82cm, between differ 2.71cm.
The pears of table 1, apple, peach, wild 1 year branch rugosity of mountain a surname walnut and panel length investigation
Therefore 10 buds are collected with every part of resource, 2 branches are cut, collect small bag length at least 30cm, width is extremely
Few 3cm.The height for determining therefrom that each branch unit bag 10 is 30-38cm, the span 3-5cm of arch support, and each resource is adopted
5-15 parallel branch unit bags 15 can be pasted on collection pad 10.
Embodiment 3
Test and carried out in August in 2015 in Jiangsu Province agricultural Ke Yuan theatre Germplasm Resources within 29th, collection variety selection ' Soviet Union
It is emerald green No. 1 ', the long 30cm of branch is often handled, every 6 buds, 10 altogether, is often handled in triplicate.
Experimental group：The portable Fruit Tree Resources provided using embodiment 1 gather bag.
Control group：Wrapped up using wet cloth, in one polybag of overcoat.
Master sample：Master sample is tree without any treated branch.
Detecting instrument：
To be checked and examined using HTC-1 digital display Hygrothermographs, specification is -50-70 DEG C of temperature measurement range, and precision is 0.1 DEG C,
Moisture measurement 10-99 (RH), precision are 1 (RH), humiture change in real-time measurement experiment group and control group bag.
Experimentation：
After experimental group and control group branch sampling packaging, it is exposed to the sun 8 hours (8 outside holding chamber:00-18:00), then put again
Put indoor shady place 24 hours, finally 3-5 DEG C of placement refrigerator refrigerator 24 hours.Master sample is the upper branch on-the-spot test of tree, often
Processing is in triplicate.
Heat insulating ability determines
Temperature in bag is carried out using HTC-1 digital displays Hygrothermograph to monitor in real time, 2 is the results are shown in Table, using the portable of the present invention
Inherent outdoor 38.8 DEG C of the highest temperature of formula fruit tree germplasm collection bag, temperature is 12.9 DEG C in bag, and temperature is than extraneous in control group bag
Temperature is high, from 8:00-18:Between 00, temperature is maintained at 2.3-17.9 DEG C in experimental group bag.
Group and control group temperature diurnal variation (unit DEG C) in outdoor bag are tested in the Room of table 2.
| 8：00 | 10：00 | 12：00 | 14：00 | 16：00 | 18：00 | |
| Experimental group | 2.3 | 7.8 | 9.0 | 12.9 | 15.8 | 17.9 |
| Control group | 31.9 | 34.9 | 38.6 | 39.1 | 36.6 | 34.2 |
| Master sample | 33.4 | 35.2 | 37.2 | 38.8 | 35.6 | 31.7 |
Resistance is evaluated
After plant injury, increase, electrolyte in various degree, which occur, for cell leakage largely to exosmose, and intercellular substance concentration increases
Greatly, conductivity value is made to become big, extent of injury is light, membrane permeability increase mild degree, and permeability change is reversible, easily recovers normal, instead
It, it is impossible to recover normal.
Electrolyte Leakage Rate (REC) determines.With reference to electrical conductivity method [Shen De thread fruit breedings experimental technique [M] Beijing:In
Agricultural publishing house of state, 1992:66-69], the branch after cryogenic freezing is handled is cut into 2mm segment, then weighs the throwing of 2g samples
Enter triangular flask, and add 50mL distilled water, after soaking 24h, you can determine the electrical conductivity of leachate.Test material need to set repetition 3
Each sample that is secondary, then will being determined after low-temperature treatment, is placed in water-bath and boils 1h, kills tissue, then adds distilled water supplement
To quantifying for original solution, its conductivity is determined after static cooling, such as formula (1).
Y=(S1/S2) × 100% (1)
In formula (1), Y represents relative electrolyte leaching rate, and S1 represents just electric conductivity value, and S2 represents whole electric conductivity value.
MDA (MDA) determines.With reference to thiobarbituricacidα- (TBA) method [Liu family Yao the study course of plant physiology experiments [M]
Beijing:Higher Education Publishing House, 2010:72-75.], the examination material 0.1g shredded is weighed, adds 2mL 10%TCA and a small amount of quartz
Sand, homogenate is ground to, then adds appropriate TCA further to grind, homogenate is transferred in 5mL centrifuge tubes, is centrifuged in 4000r/min
10min, supernatant are sample extracting solution.The supernatant 2mL of centrifugation is drawn, adds 2ml 0.6%TBA solution, is mixed, in test tube
On jump a queue, in reacting 15min on boiling water bath, rapid cooling, centrifugation.Take the light under supernatant measure 532,600,450nm wavelength
Density.Calculate such as formula (2).
MDA [μm ol/g]=(CV/W) × 103 (2)
In formula (2), C is MDA concentration (μm ol/L) in extract solution；V is extracting liquid volume (mL)；W is plant tissue fresh weight
(g)。
Influence of the different disposal of table 3. to electrical conductivity and mda content
| Processing | Electrical conductivity (%) | Mda content/(μm ol/g) |
| Experimental group | 27.05bA | 0.01bB |
| Control group | 51.67aA | 0.05aA |
| Master sample | 21.74cB | 0.01bB |
Note：Notable P≤0.05 of same column difference lowercase letter indication difference；Same column difference capitalization represents that difference is extremely notable
Property P≤0.01.
Electrical conductivity and mda content measure are carried out to experimental group, control group and master sample, as a result can from table 3
Go out, control group and experimental group branch electrical conductivity are significantly higher than master sample, and control group branch electrical conductivity conspicuousness highest；Together
Sample, the dominant highest of control group branch mda content difference, and experimental group and standard sample branch are without significant difference.By a bit may be used
See, control group branch cell leakage to a certain extent by destruction, and experimental group branch cell leakage not by
To destruction.Illustrate that experimental group is more preferable than control group store method branch vigor, be advantageous to improve scion grafting success rate.
Grafting survival is tested
Branch progress crop field grafting after being stored to experimental group and control group, master sample collection in worksite in this experiment,
It is synchronous to carry out crop field Grafting Experiment.Respectively handle and 10 buds are connect using plate budding method grafting, master sample then directly adopts fringe from field
Grafting, often handle in triplicate, experimental result is documented in table 4.
Influence of the different disposal of table 4. to graft survival rate
| Experimental group | Control group | Master sample | |
| Survival rate (%) | 86.7aA | 53.3bB | 90.0aA |
Note：Go together different notable P≤0.05 of lowercase letter indication difference；Different capitalizations of going together represent that difference is extremely notable
Property P≤0.01.
From table 4, by standard sample grafting, artificial grafting error be present, but under equal grafting error, experimental group
Scion narrow bars grafting survival is significantly higher than control group, i.e. experimental group grafting improves 62% than control composition motility rate.
Claims (4)
1. a kind of portable Fruit Tree Germplasm Resources collection bag, including Tarpauling base wad, it is characterised in that：
A) form trapezoidal spademan after the upper following extension of the Tarpauling base wad, the side of Tarpauling base wad with it is wide every
Hot heat keeper is connected as a single entity, and is provided with symmetrical packing hole in the drift angle of upper and lower spademan, and be furnished with tie string；Tarpauling base wad
Transparent bag, pouch and a pair of parallel from patch band are provided with front, from patch band by Tarpauling base wad extend to every
Hot heat keeper outer end edge；The back side of Tarpauling base wad is provided with a pair of parallel insertion Tarpauling base wad and heat-insulated guarantor
Temperature pad from patch band, should mutually be pasted with Corresponding matching from patch from patch band and Tarpauling base wad are positive；
B) collection of resources pad is cotton pad, the front of collection of resources pad be provided with it is a pair of parallel from patch band, collection of resources pad
Upper edge hides lid to be flexible, and lower edge is provided with flexible water storage bag；The reverse side of collection of resources pad be provided with it is a pair of parallel from patch band, instead
Face penetrates collection of resources pad from patch band and mutually pasted from patch band Corresponding matching with the back side of Tarpauling base wad；
C) branch unit bag includes bag body, and the side of bag body is provided with a pair from patch band, bag body from patch band and collection of resources
Pad it is positive from patch band Corresponding matching mutually paste；Also at least provided with three arch supports in each bag body, they are distributed in
Bag body top, in, bottom, the bottom of each bag body is furnished with absorbent cloth band；
D) at least paste has a branch unit bag, the absorbent cloth band of each branch unit bag bottom on the collection of resources pad described in
It is placed in the flexible water storage bag of collection of resources rim strip；The transparent bag of the Tarpauling base wad is furnished with temperature-humidity monitor,
The probe of temperature-humidity monitor is placed in a randomly selected sampling branch unit through the Tarpauling base wad in transparent bag
In bag.
2. portable Fruit Tree Germplasm Resources collection bag according to claim 1, it is characterised in that：Described heat-insulation and heat-preservation pad
It is the complex of cotton lining and double-sided aluminum foil bubble protection against the tide heat keeper；The bag body of the branch unit bag is that double-sided aluminum foil bubble is prevented
Damp heat keeper is lined with the absorbent cloth band connection of cotton liner, cotton liner and bottom, is additionally provided with the top of branch unit bag
The coverage mouth of stomata；" U " aqua storage tank that described flexible water storage bag makes for double-sided aluminum foil bubble protection against the tide heat keeper, " U " water storage
On the outside of one wall of groove between collection of resources pad by matching from patch band connection.
3. portable Fruit Tree Germplasm Resources collection bag according to claim 1, it is characterised in that：Collection of resources rim strip it is soft
It is furnished with water-absorbing sponge in property water storage bag；Equipped with storage ice bag on heat-insulation and heat-preservation pad, the one side for storing ice bag is provided with from patch band, storage
Hide being extended to from patch band and Tarpauling base wad front on heat-insulation and heat-preservation pad for ice bag and be mutually matched stickup from patch band.
4. the portable Fruit Tree Germplasm Resources collection bag according to one of claim 1-3, it is characterised in that：Each branch list
The height of first bag is 30-38cm, the span 3-5cm of arch support；It is individual parallel that 5-15 can be pasted on described collection of resources pad
Branch unit bag, interval 1cm during paste between adjacent branch unit bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610892117.4A CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610892117.4A CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
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| CN106275862A CN106275862A (en) | 2017-01-04 |
| CN106275862B true CN106275862B (en) | 2018-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610892117.4A Active CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110926848A (en) * | 2019-12-04 | 2020-03-27 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for collecting rice leaf samples by using adhesive tape |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202004002013U1 (en) * | 2004-02-11 | 2004-04-15 | Stein, Axel | Transport cover for the disposal of Christmas trees |
| CN202160842U (en) * | 2011-06-24 | 2012-03-14 | 余建桥 | Novel multifunctional pencil bag |
| US8342750B2 (en) * | 2009-06-16 | 2013-01-01 | Heinlen Christina Frances | Reusable refoldable bag |
| CN204528170U (en) * | 2015-01-29 | 2015-08-05 | 绍兴文理学院元培学院 | Succulent maintenance is set with |
| CN206087882U (en) * | 2016-10-12 | 2017-04-12 | 江苏省农业科学院 | Portable fruit tree germplasm resources gathers package |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA989614A (en) * | 1973-08-07 | 1976-05-25 | Henry A. Spencer | Container for seedlings |
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- 2016-10-12 CN CN201610892117.4A patent/CN106275862B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202004002013U1 (en) * | 2004-02-11 | 2004-04-15 | Stein, Axel | Transport cover for the disposal of Christmas trees |
| US8342750B2 (en) * | 2009-06-16 | 2013-01-01 | Heinlen Christina Frances | Reusable refoldable bag |
| CN202160842U (en) * | 2011-06-24 | 2012-03-14 | 余建桥 | Novel multifunctional pencil bag |
| CN204528170U (en) * | 2015-01-29 | 2015-08-05 | 绍兴文理学院元培学院 | Succulent maintenance is set with |
| CN206087882U (en) * | 2016-10-12 | 2017-04-12 | 江苏省农业科学院 | Portable fruit tree germplasm resources gathers package |
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| CN106275862A (en) | 2017-01-04 |
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