CN106275862A - Portable fruit tree germplasm resources collection bag - Google Patents
Portable fruit tree germplasm resources collection bag Download PDFInfo
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- CN106275862A CN106275862A CN201610892117.4A CN201610892117A CN106275862A CN 106275862 A CN106275862 A CN 106275862A CN 201610892117 A CN201610892117 A CN 201610892117A CN 106275862 A CN106275862 A CN 106275862A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D33/00—Details of, or accessories for, sacks or bags
- B65D33/16—End- or aperture-closing arrangements or devices
- B65D33/28—Strings or strip-like closures, i.e. draw closures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
- B65D81/26—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators
- B65D81/264—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators for absorbing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3888—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation wrappers or flexible containers, e.g. pouches, bags
- B65D81/3897—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation wrappers or flexible containers, e.g. pouches, bags formed of different materials, e.g. laminated or foam filling between walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
- B65D85/52—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage for living plants; for growing bulbs
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Abstract
The present invention relates to a kind of portable Fruit Tree Germplasm Resources collection bag, its Tarpauling base wad is trapezoidal spademan up and down, and side is connected with heat-insulation and heat-preservation pad, and two spademans are equipped with packing hole, front is provided with transparent bag, pouch and a pair from patch band, and the back side is provided with a pair from patch band.The front of collection of resources pad be provided with a pair from patch band, along be flexible to hide lid on it, lower edge is provided with flexible water storage bag, reverse side be provided with a pair from patch band, this is mutually pasted from patch band Corresponding matching from the back side of patch band and Tarpauling base wad.Branch unit bag includes bag, and the side of bag glues note from patch band and collection of resources pad front from patch band by a pair；Being provided with arch support in each bag, bottom is furnished with water suction strap.Branch unit bag is viscous to be posted on collection of resources pad, and its water suction strap is placed in flexible water storage bag, is furnished with temperature-humidity monitor in transparent bag, and the probe of temperature-humidity monitor is placed in a sampling branch unit bag.
Description
Technical field
The present invention relates to a kind of Fruit Tree Germplasm Resources and gather the special purpose packet of branch, a kind of portable fruit tree kind matter provides
Source gathers bag, and a kind of germ plasm resource belonging to cultivation of fruit tree thremmatology field collects specific purpose tool.
Background technology
China has abundant Fruit Tree Germplasm Resources.Ju incomplete statistics [river literary composition. perennial and asexually propagated crop kind
Matter resource-sharing research [M]. Beijing: Chinese agriculture publishing house, 2006:77-86], 45 kinds of whole world main fruit trees crop species
Contain 3893 botany kinds, origin China for 725 kinds, account for the world 18.62%；Fructus Mali pumilae, pears, Fructus Vitis viniferae, Fructus Persicae, Lee, Fructus Pruni,
The wild species quantity of large fruit tree such as Fructus Jujubae, Semen Juglandis, Citrus, Folium Eriobotryae, Arillus Longan, Fructus Litchi origin China accounts for the 56.13% of the world；45 kinds
Main fruit trees crop species cultigen has 15 kinds of origins in China or part origin in China, account for 33% [Yu Dejun. deciduous fruit tree
Taxonomy [M]. Shanghai: Science and Technology of Shanghai publishing house, 1984].Jiangsu Province Agriculture Science Institute Horticultural Research Institute is mainly engaged in Fructus Persicae, grass
The collection of Fruit Tree Germplasm Resources, preservation, the characterization and evaluations such as the certain kind of berries, Fructus Vitis viniferae, pears, wild mountain a surname Semen Juglandis grind with utilization and breeding of new variety
Study carefully, now built up national Fructus Persicae, strawberry germplasm garden and Jiangsu Province's Fruit Tree Germplasm Resources gene bank, have collected Fructus Persicae from both at home and abroad
More than 800 part, Fructus Fragariae Ananssae more than 600 part, pears more than 260 part, Fructus Vitis viniferae more than 100 part, wild mountain a surname Semen Juglandis more than 60 part etc. plant matter.
The Fruit Tree Germplasm Resources such as Fructus Persicae, Fructus Vitis viniferae, pears, wild mountain a surname Semen Juglandis are introduced a fine variety and are all used asexual reproduction method, gather from strange land
Bud is remarried and is received on affinity height stock, and this method can not only keep the inherited character of female parent, also keeps intrinsic excellent of kind
Optimum shape, secondly vegetative fruit tree is without the virgin phase, begins morning fruit phase, and vegetative Seedling does not has segregation phenomenon, and nursery stock is neat
Unanimously.
Fruit Tree Germplasm Resources acquisition time will be carried out the most throughout the year.Tradition fruit tree field is collected and is planted at present
Matter, all uses parcel scion, the most outside one plastic bag of surface cover such as wet newspaper, coil paper, wet towel, adopts in this way winter
Feasible, but winter field is collected and be can only see bare branch when planting matter, other important fruit properties, leaf characters and disease resistance
All can't see, be difficult to debate and what does not gather plant matter, therefore, optimal acquisition period is Growth status developmental stage, tree has leaf, has
Really, moreover it is possible to the resistance of observation of plant, i.e. the 7-9 month is optimal, and this stage Lingao temperature, gather scion skill bar mostly wooden in half
Changing, be difficult to storage, moisture content has lacked branch can be withered, and moisture content is many, and bud easily rots, and temperature is high in addition, and branch easily brown stain loses work
Power, gathers the branch graft survival rate returned extremely low.For the problems referred to above, a kind of new fruit tree of the highly desirable acquisition in this area is wild
Outer germ plasm resource collects bag.
Summary of the invention
It is an object of the invention to, for gathering the adverse effect that grafting is brought by environment, the most usual strick precaution at present
Measure operation is more complicated, and effect is the most not fully up to expectations, it is provided that a kind of brand-new portable Fruit Tree Resources collection bag.
The object of the present invention is achieved like this: a kind of portable Fruit Tree Germplasm Resources collection bag, including Tarpauling base
Pad, it is characterised in that:
A) forming trapezoidal spademan after the upper following extension of described Tarpauling base wad, the side of Tarpauling base wad is with wide
Heat-insulation and heat-preservation pad be connected as a single entity, be provided with the packing hole of symmetry in the drift angle of upper and lower spademan, and be furnished with tie string；Tarpauling base
The front of pad is provided with transparent bag, pouch and pair of parallel from patch band, is extended by Tarpauling base wad from patch band
To heat-insulation and heat-preservation pad outer end edge；The back side of Tarpauling base wad be provided with pair of parallel through Tarpauling base wad and every
Hot heat keeper from patch band, should mutually pasting from patch band Corresponding matching from patch band and Tarpauling base wad front；
B) collection of resources pad is cotton pad, and the front of collection of resources pad is provided with pair of parallel from patch band, collection of resources
Pad lower edge be provided with flexible water storage bag；The reverse side of collection of resources pad is provided with pair of parallel from patch band, reverse side from patch
With through collection of resources pad and with mutually the pasting from patch band Corresponding matching of the back side of Tarpauling base wad；
C) branch unit bag includes bag, the side of bag be provided with a pair from patch band, bag from patch band and resource
Gather pad front mutually glues note from patch band Corresponding matching；Also at least provided with three arch support in each bag, they divide
Cloth the top of bag, in, bottom, the bottom of each bag is furnished with water suction strap；
D) at least glue note on the collection of resources pad described in and have a branch unit bag, at the bottom of the absorbent cloth of each branch unit bag
The water suction strap in portion is placed in the flexible water storage bag of collection of resources rim strip；The transparent bag of described Tarpauling base wad is furnished with temperature
Moisture monotor, temperature-humidity monitor probe through the Tarpauling base wad in transparent bag be placed in one randomly choose adopt
In sample branch unit bag.
In the present invention: described heat-insulation and heat-preservation pad is cotton lining and the complex of double-sided aluminum foil bubble protection against the tide heat keeper；
The bag of described branch unit bag is the suction that double-sided aluminum foil bubble protection against the tide heat keeper is lined with cotton liner, cotton liner and bottom
Water strap connects, and the top of branch unit bag is additionally provided with the coverage mouth of air-vent；Described flexible water storage bag is double-sided aluminum foil gas
Steep " U " aqua storage tank that moistureproof heat keeper makes, certainly pasted by mate with between collection of resources pad outside a wall of " U " aqua storage tank
Strap connects.
In the present invention: the flexible water storage bag of collection of resources rim strip is furnished with water-absorbing sponge；It is furnished with storage on heat-insulation and heat-preservation pad
Hiding ice bag, the one side of storage ice bag is provided with from patch band, extending to Tarpauling base wad front from patch band of storage ice bag
It is mutually matched stickup from patch band on heat-insulation and heat-preservation pad.
In the present invention: the height of each branch unit bag is 30-38cm, span 3-5cm of arch support；Described money
Source gathers that pad is upper can glue note 5-15 parallel branch unit bag, interval 1cm between adjacent branch unit bag when gluing note.
It is an advantage of the current invention that: use portable Fruit Tree Resources collection bag, be suitable for going field acquisition resource throughout the year,
And every part of the most independent subpackage of germ plasm resource, it is ensured that introduce a fine variety accuracy；Scion, portable Fruit Tree Resources is gathered at autumn in summer high temperature
Gather bag scion bud vigor, be remarkably improved grafting Cheng Li, it is ensured that successful introduction rate.
Experiment shows, between pears, Fructus Mali pumilae, Fructus Persicae, wild mountain a surname Semen Juglandis, branch thick and panel length exist notable difference, same
There is also difference between fruit trees different cultivars, central annual young sprout rugosity is the most carefully ' rain liquid distilled from honeysuckle flowers or lotus leaves ' Fructus Persicae, for 0.32cm, the most slightly
Be ' Shi Lai ' wild mountain a surname Semen Juglandis, for 1.24cm, between differ 0.82cm；Panel length is the shortest is ' Nagafu No.2 ' Fructus Mali pumilae, for
2.11cm, the longest is ' dragon's fountain Suli pear ', for 4.82cm, between differ 2.71cm.Therefore, collect 10 with every part of resource
Bud, cuts and cuts 2 branches, collect little bag length at least 30cm, width at least 3cm.Determine therefrom that the height of each branch unit bag 10
Degree is 30-38cm, span 3-5cm of arch support, and each collection of resources pad can glue note 5-15 parallel branch unit
Bag 10.
It is also an advantage of the present invention that, use the portable fruit tree kind matter collection of the present invention to wrap in the outdoor highest temperature 38.8
DEG C, in bag, temperature is only 12.9 DEG C, and between 8:00-18:00, in bag, temperature is maintained at 2.3-17.9 DEG C.Scion electrical conductivity, third
The significantly high experimental group of dialdehyde content matched group, illustrates experimental group scion cell leakage and is not affected by destroying.Through this technology
Link makes field grafting survival force rate matched group improve 62%, illustrates that this technology not only ensures to introduce a fine variety purity, success rate, also save
Save a large amount of human and material resources and quality time, provide more preferably sound assurance for resource collection work.
Accompanying drawing explanation
Fig. 1: be the Facad structure schematic diagram of the embodiment of the present invention；
Fig. 2: be the inverse layer structure schematic diagram of the embodiment of the present invention；
Fig. 3: be branch unit bag arrangement mode schematic diagram on collection of resources pad in the embodiment of the present invention.
In figure: 1, heat-insulation and heat-preservation pad, 2, Tarpauling base wad, 3, the public sheet that band hooks in viscose cloth tape, 4, transparent bag, 5,
Temperature-humidity monitor, 6, kit bag, 7, pen bag, 8, storage ice bag, 9, in viscose cloth tape the master slice of band floss, 10, collection of resources pad,
11, flexible water storage bag, 12, lead-in wire, 13, tie string, 14, the flexible lid that hides, 15, branch unit bag, 16, arch support, 17, inhale
Water strap.
Detailed description of the invention
The nonrestrictive concrete structure disclosing the embodiment of the present invention of accompanying drawing, makees into one the present invention below in conjunction with the accompanying drawings
Step ground describes.
Embodiment 1
From Fig. 1 and Fig. 2, the present invention includes Tarpauling base wad 2, the upper following extension of described Tarpauling base wad 2
The trapezoidal spademan of rear formation, the side of Tarpauling base wad 2 is connected as a single entity with wide heat-insulation and heat-preservation pad 1, the drift angle of upper and lower spademan
Inside it is provided with the packing hole of symmetry, and is furnished with tie string 13.
In FIG, the front of Tarpauling base wad 2 is provided with transparent bag 4, pouch and pair of parallel from patch
Band, is extended to its end edge by Tarpauling base wad 2 through heat-insulation and heat-preservation pad 1 from patch band, and wherein, pouch includes kit bag
6 and pen bag 7, hook public sheet 3 (band floss master slice 9 can also be selected) from patch band for band in patch band.It is furnished with on heat-insulation and heat-preservation pad 1
Storage ice bag 8, the one side of storage ice bag 8 is provided with from patch band, when Tarpauling base wad 2 front from patch band be from patch
When in band, band hooks public sheet 3, that preserves ice bag 8 can only select matched band floss master slice 9 from patch band.
In fig. 2, the back side of Tarpauling base wad 2 is provided with pair of parallel through Tarpauling base wad 2 and heat-insulation and heat-preservation
Pad 1 from patch band, select the band floss master slice 9 with the pairing of Tarpauling base wad 2 front (when Tarpauling base wad 2 from patch band
When front is for selecting band floss master slice 9, it is also possible to select its band matched to hook public sheet 3), in using, being somebody's turn to do can be with waterproof from patch band
Mutually pasting from patch band 3 Corresponding matching of canvas base wad 2 front, so may insure that when heat-insulation and heat-preservation pad 1 is rolled, the back side with
Front can mutually glue note from patch band.
In fig. 2, collection of resources pad 10 is cotton pad, and the front of collection of resources pad 10 is provided with pair of parallel from patch band
3, hook public sheet 3 (band floss master slice 9 can also be selected) from patch band for band in patch band, the upper edge of collection of resources pad 10 is flexible
Hiding lid 14, lower edge is provided with flexible water storage bag 11, and the reverse side of collection of resources pad 10 is provided with pair of parallel from patch band, waterproof sail
Cloth base wad 2 is band floss master slice 9 from patch band, and the net of collection of resources pad reverse side can only select matched band to hook from patch band
Public sheet 3.
It is visible that Fig. 2 combines Fig. 3, and branch unit bag 15 includes bag, the one side of bag be provided with a pair from patch band, bag
Note is mutually glued from patch band Corresponding matching from patch band and collection of resources pad 10 front；In each branch unit bag 15 the most at least
Be provided with three arch support 16, they be distributed in bag top, in, bottom, the bottom of each bag be furnished with water suction strap 17.Often
Note can be glued on individual collection of resources pad 10 and have the interval 1cm between 5-15 branch unit bag 15 adjacent branch unit bag 15, often
Water suction strap 17 bottom individual branch unit bag 15 is placed in the flexible water storage bag 11 of collection of resources rim strip 10；Described waterproof sail
The transparent bag of cloth base wad 2 is furnished with temperature-humidity monitor 5, the waterproof sail of 4 in transparent bag of popping one's head in of temperature-humidity monitor 5
Cloth base wad 2 is placed in a sampling branch unit bag 15 randomly choosed by lead-in wire 12.
In the present embodiment, described heat-insulation and heat-preservation pad 1 is the compound of cotton lining and double-sided aluminum foil bubble protection against the tide heat keeper
Body.The bag of described branch unit bag 15 is that double-sided aluminum foil bubble protection against the tide heat keeper is lined with cotton liner, cotton liner and the end
The water suction strap 17 in portion connects.Described flexible water storage bag 11 is " U " aqua storage tank that double-sided aluminum foil bubble protection against the tide heat keeper makes,
With being connected from patch band by coupling between collection of resources pad 10 outside one wall of " U " aqua storage tank, flexible water storage bag 11 is joined
There is water-absorbing sponge.
In the present embodiment, in order to improve the effect of cooling moisture-retaining, collection of resources rim strip 10 front can also configure storage
Ice bag 8.
Embodiment 2:
The size of branch unit bag 15 determines
Test agricultural Ke Yuan theatre, Jiangsu Province Germplasm Resources, country Fructus Persicae Germplasm Resources, academy of agricultural sciences six directions animals and plants
Proving ground, Dasha River orchard, Xuzhou are acquired investigation.Resource selects the peripheral long shoot of the tree vigo(u)r golden mean of the Confucian school, robust growth, by length
Calculating panel length, rugosity oil ga(u)ge kind of calliper divided by joint number, every kind repeats for 3 times, repeats 10 branches every time.
As shown in Table 1, between different fruit trees there are notable difference, same class fruit tree difference product in branch thick and panel length
Difference is there is also between kind；In the middle of pears, Fructus Mali pumilae, Fructus Persicae, wild mountain a surname Semen Juglandis, annual young sprout rugosity is the most carefully ' rain liquid distilled from honeysuckle flowers or lotus leaves ' Fructus Persicae, for
0.32cm, the thickest is ' Shi Lai ' wild mountain a surname Semen Juglandis, for 1.24cm, between differ 0.82cm；It is ' long by rich 2 that panel length is the shortest is
Number ' Fructus Mali pumilae, for 2.11cm, the longest is ' dragon's fountain Suli pear ', for 4.82cm, between differ 2.71cm.
Table 1 pears, Fructus Mali pumilae, Fructus Persicae, wild mountain 1 year branch rugosity of a surname Semen Juglandis and panel length investigation
Therefore, to collect 10 buds with every part of resource, cut and cut 2 branches, collect little bag length at least 30cm, width is extremely
Few 3cm.The height determining therefrom that each branch unit bag 10 is 30-38cm, and span 3-5cm of arch support, each resource is adopted
Note 5-15 parallel branch unit bag 15 can be glued on collection pad 10.
Embodiment 3
Test and carry out at agricultural Ke Yuan theatre, Jiangsu Province Germplasm Resources on August 29th, 2015, gather Fruit variety ' Soviet Union
Emerald green No. 1 ', often process the long 30cm of branch, every 6 buds, 10 altogether, often process in triplicate.
Experimental group: utilize the portable Fruit Tree Resources collection bag that embodiment 1 provides.
Matched group: utilize wet cloth to wrap up, at one plastic bag of overcoat.
Master sample: master sample is that tree is without any branch processed.
Detecting instrument:
Using HTC-1 digital display Hygrothermograph to check and examine, specification is temperature measurement range-50-70 DEG C, and precision is 0.1 DEG C,
Moisture measurement 10-99 (RH), precision is 1 (RH), humiture change in real-time experiments of measuring group and matched group bag.
Experimentation:
After experimental group and matched group branch are sampled packaging, be exposed to the sun outside holding chamber 8 hours (8:00-18:00), puts the most again
Put indoor shady place 24 hours, finally place 3-5 DEG C of refrigerator refrigerator 24 hours.Master sample is the upper branch on-the-spot test of tree, often
Process in triplicate.
Heat insulating ability measures
In using HTC-1 digital display Hygrothermograph to carry out bag, temperature is monitored in real time, the results are shown in Table 2, and the employing present invention's is portable
Outdoor, the formula fruit tree kind matter collection bag inherence highest temperature 38.8 DEG C, in bag, temperature is 12.9 DEG C, and in matched group bag, temperature is than the external world
Temperature is high, and between 8:00-18:00, in experimental group bag, temperature is maintained at 2.3-17.9 DEG C.
Group and temperature diurnal variation (unit DEG C) in matched group bag in the open are tested in table 2. Room
| 8:00 | 10:00 | 12:00 | 14:00 | 16:00 | 18:00 | |
| Experimental group | 2.3 | 7.8 | 9.0 | 12.9 | 15.8 | 17.9 |
| Matched group | 31.9 | 34.9 | 38.6 | 39.1 | 36.6 | 34.2 |
| Master sample | 33.4 | 35.2 | 37.2 | 38.8 | 35.6 | 31.7 |
Resistance is evaluated
After plant is injured, cell leakage occurs to increase in various degree, and electrolyte can exosmose in a large number, and intercellular substance concentration increases
Greatly, making conductivity value become big, extent of injury is light, and membrane permeability increases mild degree, and property change reversible thoroughly, easily recovers normal, instead
It, it is impossible to recover normal.
Electrolyte Leakage Rate (REC) measures.Reference electrical conductivity method [Shen Dexu. fruit breeding experimental technique [M]. Beijing: in
Agricultural publishing house of state, 1992:66-69], the branch after freezing being processed is cut into the segment of 2mm, then weighs 2g sample and throws
Enter triangular flask, and add 50mL distilled water, after soaking 24h, the electrical conductivity of leachate can be measured.Test material need to set repetition 3
Each sample that is secondary, that then will measure after K cryogenic treatment, is placed in water-bath and boils 1h, kills tissue, then it is supplementary to add distilled water
Quantitative to original solution, measures its conductivity, such as formula (1) after non-shock chilling.
Y=(S1/S2) × 100% (1)
In formula (1), Y represents relative electrolyte leaching rate, and S1 represents just electric conductivity value, and S2 represents whole electric conductivity value.
Malonaldehyde (MDA) measures.Reference thiobarbituricacidα-(TBA) method [Liu Jiayao. plant physiology experiment study course [M].
Beijing: Higher Education Publishing House, 2010:72-75.], weigh the examination material 0.1g shredded, add 2mL 10%TCA and a small amount of quartz
Sand, is ground to homogenate, then adds appropriate TCA and grind further, and homogenate is transferred in 5mL centrifuge tube, is centrifuged at 4000r/min
10min, supernatant is sample extracting solution.Drawing centrifugal supernatant 2mL, add 2ml 0.6%TBA solution, mixing, at test tube
On jump a queue, on boiling water bath, react 15min, cool down rapidly, centrifugal.Take supernatant measure 532,600, light under 450nm wavelength
Density.Calculate such as formula (2).
MDA [μm ol/g]=(CV/W) × 103 (2)
In formula (2), C is MDA concentration (μm ol/L) in extracting solution；V is extracting liquid volume (mL)；W is plant tissue fresh weight
(g)。
Table 3. different disposal is on electrical conductivity and the impact of mda content
| Process | Electrical conductivity (%) | Mda content/(μm ol/g) |
| Experimental group | 27.05bA | 0.01bB |
| Matched group | 51.67aA | 0.05aA |
| Master sample | 21.74cB | 0.01bB |
Note: same column difference lowercase alphabet shows significant difference P≤0.05；Same column difference capitalization represents that difference is the most notable
Property P≤0.01.
Experimental group, matched group and master sample carrying out electrical conductivity and mda content measures, result can from table 3
Going out, matched group and experimental group branch electrical conductivity are significantly higher than master sample, and matched group branch electrical conductivity significance is the highest；With
Sample, matched group branch mda content difference is dominant the highest, and experimental group and standard sample branch are without significant difference.By a bit may be used
Seeing, matched group branch cell leakage is being subjected to destruction to a certain extent, and experimental group branch cell leakage is not subject to
To destroying.Illustrate that experimental group is more preferable than matched group store method branch vigor, be conducive to improving scion grafting success rate.
Grafting survival is tested
After preserving experimental group and matched group, branch carries out land for growing field crops grafting, and master sample is collection in worksite when this test,
Synchronization carries out land for growing field crops Grafting Experiment.Each process uses plate budding method grafting to connect 10 buds, and fringe then directly adopted by master sample from field
Grafting, often processes in triplicate, and experimental result is recorded in table 4.
The impact on graft survival rate of table 4. different disposal
| Experimental group | Matched group | Master sample | |
| Survival rate (%) | 86.7aA | 53.3bB | 90.0aA |
Note: colleague is different, and lowercase alphabet shows significant difference P≤0.05；Colleague is different, and capitalization represents that difference is the most notable
Property P≤0.01.
From table 4, by standard sample grafting, there is artificial grafting error, but under equal grafting error, experimental group
Scion connects bar grafting survival and is significantly higher than matched group, i.e. experimental group grafting improves 62% than comparison composition motility rate.
Claims (4)
1. a portable Fruit Tree Germplasm Resources collection bag, including Tarpauling base wad, it is characterised in that:
A) form trapezoidal spademan after the upper following extension of described Tarpauling base wad, the side of Tarpauling base wad with wide every
Hot heat keeper is connected as a single entity, and is provided with the packing hole of symmetry, and is furnished with tie string in the drift angle of upper and lower spademan；Tarpauling base wad
Front is provided with transparent bag, pouch and pair of parallel from patch band, from patch band by Tarpauling base wad extend to every
Hot heat keeper outer end edge；The back side of Tarpauling base wad is provided with pair of parallel through Tarpauling base wad and heat insulation guarantor
Temperature pad from patch band, should mutually pasting from patch band Corresponding matching from patch band and Tarpauling base wad front；
B) collection of resources pad is cotton pad, and the front of collection of resources pad is provided with pair of parallel from patch band, collection of resources pad
Upper edge is flexible screening lid, and lower edge is provided with flexible water storage bag；The reverse side of collection of resources pad is provided with pair of parallel from patch band, instead
Face from patch band through collection of resources pad and with mutually the pasting from patch band Corresponding matching of the back side of Tarpauling base wad；
C) branch unit bag includes bag, the side of bag be provided with a pair from patch band, bag from patch band and collection of resources
That pads front mutually glues note from patch band Corresponding matching；Also at least provided with three arch support in each bag, they are distributed in
The top of bag, in, bottom, the bottom of each bag is furnished with water suction strap；
D) at least glue note on the collection of resources pad described in and have a branch unit bag, the water suction strap bottom each branch unit bag
It is placed in the flexible water storage bag of collection of resources rim strip；The transparent bag of described Tarpauling base wad is furnished with temperature-humidity monitor,
The probe of temperature-humidity monitor is placed in a sampling branch unit randomly choosed through the Tarpauling base wad in transparent bag
In Dai.
Portable Fruit Tree Germplasm Resources collection bag the most according to claim 1, it is characterised in that: described heat-insulation and heat-preservation pad
It is cotton lining and the complex of double-sided aluminum foil bubble protection against the tide heat keeper；The bag of described branch unit bag is that double-sided aluminum foil bubble is prevented
Tide heat keeper is lined with cotton liner, and cotton liner is connected with the water suction strap of bottom, and the top of branch unit bag is additionally provided with
The coverage mouth of pore；Described flexible water storage bag is " U " aqua storage tank that double-sided aluminum foil bubble protection against the tide heat keeper makes, " U " water storage
With being connected from patch band by coupling between collection of resources pad outside one wall of groove.
Portable Fruit Tree Germplasm Resources collection bag the most according to claim 1, it is characterised in that: collection of resources rim strip soft
Property water storage bag is furnished with water-absorbing sponge；Being furnished with storage ice bag on heat-insulation and heat-preservation pad, the one side of storage ice bag is provided with from patch band, storage
Hide ice bag extend to from patch band and Tarpauling base wad front be mutually matched stickup from patch band on heat-insulation and heat-preservation pad.
4. according to the portable Fruit Tree Germplasm Resources collection bag one of claim 1-3 Suo Shu, it is characterised in that: each branch list
The height of unit's bag is 30-38cm, span 3-5cm of arch support；Note 5-15 can be glued individual parallel on described collection of resources pad
Branch unit bag, interval 1cm between adjacent branch unit bag during viscous note.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610892117.4A CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610892117.4A CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
Publications (2)
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| CN106275862A true CN106275862A (en) | 2017-01-04 |
| CN106275862B CN106275862B (en) | 2018-03-16 |
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| CN201610892117.4A Active CN106275862B (en) | 2016-10-12 | 2016-10-12 | Portable fruit tree germplasm resources collection bag |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110926848A (en) * | 2019-12-04 | 2020-03-27 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for collecting rice leaf samples by using adhesive tape |
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| GB1535974A (en) * | 1973-08-07 | 1978-12-13 | Spencer H | Growing of seedlings |
| DE202004002013U1 (en) * | 2004-02-11 | 2004-04-15 | Stein, Axel | Transport cover for the disposal of Christmas trees |
| CN202160842U (en) * | 2011-06-24 | 2012-03-14 | 余建桥 | Novel multifunctional pencil bag |
| US8342750B2 (en) * | 2009-06-16 | 2013-01-01 | Heinlen Christina Frances | Reusable refoldable bag |
| CN204528170U (en) * | 2015-01-29 | 2015-08-05 | 绍兴文理学院元培学院 | Succulent maintenance is set with |
| CN206087882U (en) * | 2016-10-12 | 2017-04-12 | 江苏省农业科学院 | Portable fruit tree germplasm resources gathers package |
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2016
- 2016-10-12 CN CN201610892117.4A patent/CN106275862B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1535974A (en) * | 1973-08-07 | 1978-12-13 | Spencer H | Growing of seedlings |
| DE202004002013U1 (en) * | 2004-02-11 | 2004-04-15 | Stein, Axel | Transport cover for the disposal of Christmas trees |
| US8342750B2 (en) * | 2009-06-16 | 2013-01-01 | Heinlen Christina Frances | Reusable refoldable bag |
| CN202160842U (en) * | 2011-06-24 | 2012-03-14 | 余建桥 | Novel multifunctional pencil bag |
| CN204528170U (en) * | 2015-01-29 | 2015-08-05 | 绍兴文理学院元培学院 | Succulent maintenance is set with |
| CN206087882U (en) * | 2016-10-12 | 2017-04-12 | 江苏省农业科学院 | Portable fruit tree germplasm resources gathers package |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110926848A (en) * | 2019-12-04 | 2020-03-27 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for collecting rice leaf samples by using adhesive tape |
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| Publication number | Publication date |
|---|---|
| CN106275862B (en) | 2018-03-16 |
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